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Effects of Forest Gap Disturbance on Plant Species Diversity and Stability in Regeneration Layers of Castanopsis kawakamii Natural Forests 林窗干扰对川槠天然林更新层植物物种多样性和稳定性的影响
Pub Date : 2012-01-01 DOI: 10.3724/SP.J.1142.2012.20133
He Zhongsheng, Jin-fu Liu, Shi-qun Zheng, W. Hong, Zeyan Wu, Dao-wei Xu, Cheng-zhen Wu
The effect of forest gap disturbance on the diversity and stability of species in the regeneration layers of natural Castanopsis kawakamii forest were studied by applying biodiversity indices and improved M.Godron's stability methods in C.kawakamii natural reserve.The results showed that: forest gap disturbance improved its micro-environment,as reflected in the differences in importance values and biodiversity in the tree and shrub layers of forest gap and understory.The biodiversity indices of richness index R,domination index D,Shannon-Wiener diversity index H,and evenness-dominance-abundance index Z in the forest gap tree layer were higher than those of the forest understory,while evenness index Js and community dominance C were lower than those of the forest understory.The biodiversity indices of R,D,H,J s,and Z in the forest gap shrub layer were higher than those of the forest understory,while C were lower than those of forest understory.Biodiversity in the forest gap was richer with less evident ecological dominant species,which indicated that the population in the forest gap was developing in the direction of complexity and homogeneity.The similarity coefficients of C j,C s,and C N were relatively low,while C mH was relatively high in the tree layer of the forest gap and understory,which demonstrated that the forest gap promoted the regeneration of trees.The similarity coefficients of C j,C s,C N,and C mH were relatively high without exception and the species composition in the forest gap were basically identical with those of the understory.According to the theory of M.Godron and the simulated the optimum stability model,the logarithm model and quadratic objective function model were the best stability models in tree layer of the forest gap and understory,respectively,and the optimum stability model of the shrub layer in both the forest gap and understory was the logarithm model.The plants in the tree layer of the forest gap were stable,but were unstable in the understory.The plants in the shrub layer of both the forest gap and understory were stable.The importance value of Litsea subcoriacea was the highest in the tree layer of the forest gap and understory,which showed that it played an important role in forest regeneration and would likely have a greater impact on the regeneration of C.kawakamii population.The importance value of C.kawakamii was relatively low in the tree layer of the forest understory,which impacted its regeneration.However,forest gap disturbance improved the upward growth and importance value of C.kawakamii,which could achieve the regeneration and restoration of the C.kawakamii population effectively.
采用生物多样性指数和改良m.g adron稳定性法,研究了林窗干扰对川川栲自然保护区再生层物种多样性和稳定性的影响。结果表明:林隙扰动改善了林隙与林下乔灌木层的微环境,体现在林隙与林下乔灌木层的重要值和生物多样性差异上;林隙乔木层的丰富度指数R、优势度指数D、Shannon-Wiener多样性指数H和均匀度-优势度-丰度指数Z均高于林下,而均匀度指数Js和群落优势度C均低于林下。林隙灌丛层的R、D、H、J、s、Z的生物多样性指数高于林下,而C的生物多样性指数低于林下。林隙内生物多样性丰富,生态优势种不明显,表明林隙内种群正朝着复杂性和均匀性方向发展。林隙和林下乔木层的C j、C s和C N相似系数相对较低,而C mH相似系数相对较高,说明林隙促进了树木的更新。cj、cs、cn、cmh的相似系数均较高,林隙内的物种组成与林下基本一致。根据M.Godron理论和模拟的最优稳定性模型,对数模型和二次目标函数模型分别为林隙乔木层和林下层的最佳稳定性模型,林隙灌丛层和林下层的最优稳定性模型均为对数模型。林隙乔木层植物稳定,林下植物不稳定。林隙灌丛层和林下植被均保持稳定。在林窗和林下乔木层中,山柴的重要值最高,说明山柴在森林更新中具有重要作用,可能对川川木种群的更新产生较大影响。川川木在林下乔木层中的重要值较低,影响了其更新。林隙干扰提高了川川冷杉的向上生长和重要值,可以有效地实现川川冷杉种群的更新和恢复。
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引用次数: 2
Tracking Analysis of Forest Spatial Structure Change after Different Selective Cutting Intensities in a Natural Forest 天然林不同选择性采伐强度后森林空间结构变化的跟踪分析
Pub Date : 2012-01-01 DOI: 10.3724/SP.J.1142.2012.30230
Hui-Rong Chen, Xin-nian Zhou, Ruichu Cai, Xi-sheng Hu, Zhi-long Wu, Cheng-jun Zhou
Based on long-term tracking retest data and three structure parameters(mingling intensity,neighborhood comparison and uniform angle index),we investigated the effects of four different selective cutting intensities(low intensity of 13.0% in volume,medium intensity of 29.1%,high intensity of 45.8%,and over-high intensity of 67.1%) on forest spatial structure change in a natural forest.After low and medium selective cutting forest,plot average mingling and neighborhood comparison increased while high and over-high selective cutting decreased.Selective cutting did not change the forest spatial distribution pattern.However,the variety of forest plot showed aggregated distribution,and medium selective cutting in random distribution state forest number increased considerably.After low and medium selective cutting,forest spatial structure was optimized in general.
基于长期跟踪复测数据和3个结构参数(混交强度、邻域对比和均匀角度指数),研究了4种不同的选择性采伐强度(低采伐强度为13.0%、中采伐强度为29.1%、高采伐强度为45.8%、过高采伐强度为67.1%)对天然林森林空间结构变化的影响。低、中选择性采伐后样地平均混交率和邻域比较增加,高、过高选择性采伐降低。选择性采伐没有改变森林的空间分布格局。而样地种类呈聚集分布,随机分布状态的中选择性采伐林分数量明显增加。低、中选择性采伐后,森林空间结构总体上得到优化。
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引用次数: 2
Production and Identification of Interspecific Hybrids between Chinese Cabbage and Purple Cabbage 大白菜与紫甘蓝种间杂交种的生产与鉴定
Pub Date : 2012-01-01 DOI: 10.3724/SP.J.1142.2012.40407
H. Qiao, Fei Li, Shujiang Zhang, Shifan Zhang, Hui Zhang, R. Sun
Interspecific hybridizations between different varieties of Brassica rapa L.ssp.peki-nensis(Lour.) Olsson and Brassica oleracea var.capitata L.were carried out.The hybrids were obtained by successive bud pollination and ovule culture and tested by means of identification by cytology.Results showed that 57 plants were obtained by interspecific hybridization between B.rapa L.ssp.pekinensis and B.oleracea var.capitata L.Cytological identification and pollen characteristics survey revealed that 47 of the hybrids had the anticipated chromosomes(2n=19) and pollen fertility was aborted;while 6 hybrids had 38chromosomes and were fertile.The BC1 plants were obtained through back cross between fertile hybrids F1 and B.rapa L.ssp.pekinensis.Field investigation showed that all F1 hybrids had many characteristics from both parents,the BC1 plants had obvious heading and were similar to B.rapa L.ssp.pekinensis.
北京芸苔不同品种种间杂交研究奥尔森(Olsson)和变种甘蓝(芸苔)进行了试验。杂种是通过连续的芽授粉和胚珠培养获得的,并通过细胞学鉴定进行了检验。结果表明,北京刺花甘蓝与花椒甘蓝种间杂交得到57株,细胞学鉴定和花粉性状调查表明,47株杂种具有预期的染色体(2n=19),花粉不育;6株杂种具有38条染色体,可育。利用可育杂种F1与北京拉帕回交获得BC1植株,田间调查结果表明,所有F1杂种均具有亲本的许多性状,BC1植株抽头明显,与北京拉帕相似。
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引用次数: 2
Interspecific Correlations among Bryophytes and among Bryophytes and Vascular Plants in Two Scales in Jinchuan Peatland 金川泥炭地两尺度苔藓植物种间关系及苔藓植物与维管植物种间关系
Pub Date : 2012-01-01 DOI: 10.3724/SP.J.1142.2012.50459
Jing Zeng, Z. Bu, Jinze Ma, Gao-Lin Zhao, C. Long, Wei Li, Binggang Zhang, Hua-fang Qu, Lu Feng
Two quadrat scales,1 m×1 m and 25 cm ×25 cm,were established to investigate the vegetation of the Jinchuan Peatland.Interspecific correlations among 9 bryophytes and 30 main vascular plants were analyzed by X 2-test,Pearson correlation,and Spearman rank correlation quantitatively.There were significantly negative associations among three main peatmosses: Sphagnum subsecundum,S.palustre,and S.capillifolium,each of which occupied different habitats.Accordingly,four ecological species groups were classified.Spearman rank correlation analysis showed that positive association amounted to 49.53% of the 741 species pairs in the large quadrats,and more than 41.14% of the 666 species pairs in the small quadrats.This study suggests that quadrat size played a crucial role in analyzing interspecific associations in peatlands,with great disparity in body size of different plant groups.It appears more suitable to analyze interspecific associations among bryophytes and among bryophytes and-vascular plants with small and large quadrats,respectively.
建立了1 m×1 m和25 cm ×25 cm两个样方尺度对金川泥炭地植被进行调查。采用x2检验、Pearson相关和Spearman秩相关定量分析了9种苔藓植物与30种主要维管植物的种间相关性。三种主要的泥炭属植物:次次生泥炭属(Sphagnum subsecundum);它们分别占据不同的生境。据此,划分出4个生态类群。Spearman秩相关分析结果显示,大样方741种对中有49.53%为正相关,小样方666种对中有41.14%以上为正相关。本研究表明,样方大小在泥炭地不同植物类群的种间关联分析中起着至关重要的作用。用小样方和大样方分别分析苔藓植物间和苔藓植物与维管束植物间的种间关联更合适。
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引用次数: 3
Functional Roles of Heat Shock Proteins 90-3(Hsp90-3) in Senecio scandens Buch.-Ham.ex D.Don Based on Its Bioinformatics 热休克蛋白90-3(Hsp90-3)在火腿中的功能作用基于它的生物信息学
Pub Date : 2012-01-01 DOI: 10.3724/SP.J.1142.2012.40385
Junjiao Ping, Zhen Zhang, Zhenwu Cai, Xiangying Tang, G. Qian
Heat shock proteins(Hsp),representing an important molecular chaperone in eukaryotic cells,is a common response to development,stress resistance,signal transduction and evolution of plants.The relationship between the structure and functional roles was elucidated in Hsp90 based on the generation of full-length cDNAs from Senecio scandens Buch.-Ham.ex D.Don.Sequence analysis of Hsp90-3 gene indicated that it shared 93.71% identity with Arabidopsis thaliana(GenBank accession: NP_200412.1),encoding a protein composed of 699 amino acid residues with the predicted molecular weight of 79.78 kD and theoretical isoelectric point of 5.08.Moreover,the distribution of Hsp90-3 was involved in the endomembrane system such as nuclei,peroxisomes,chloroplast thylakoid membranes,and chloroplast matrices in the present study.Three-dimensional measurement revealed that the Hsp90-3 protein was composed of three structural domains and one link region.These results suggested that Hsp90-3 played a critical role in molecular chaperone,signal transduction,transcriptional regulation and stress-response in higher plants.
热休克蛋白(Heat shock proteins, Hsp)是真核细胞中一种重要的分子伴侣蛋白,是植物在发育、抗逆性、信号转导和进化过程中的共同反应。基于Senecio scandens Buch.-Ham全长cdna的生成,我们在Hsp90中阐明了结构与功能作用之间的关系。Hsp90-3基因序列分析表明,该基因与拟南芥(GenBank登录:NP_200412.1)同源性为93.71%,编码一个由699个氨基酸残基组成的蛋白,预测分子量为79.78 kD,理论等电点为5.08。此外,在本研究中,Hsp90-3的分布涉及细胞核、过氧化物酶体、叶绿体类囊体膜和叶绿体基质等膜系统。三维测量表明,Hsp90-3蛋白由3个结构域和1个连接区组成。这些结果表明,Hsp90-3在高等植物的分子伴侣、信号转导、转录调控和胁迫应答中发挥着重要作用。
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引用次数: 0
Differential Expression and Gene Cloning of Ferritin from Nostoc flagelliformeSubjected to Desiccation 干燥条件下赤毛念珠铁蛋白的差异表达及基因克隆
Pub Date : 2012-01-01 DOI: 10.3724/SP.J.1142.2012.10072
W. Liang, Guangling Jiao, Youwen Zhou, Yaping Zhang, Wei Chen
Nostoc flagelliforme is a terrestrial nitrogen-fixing cyanobacterium with strong drought adaptability.We performed 2-DE analysis,gel image analysis and MALDI-TOF-TOF/MS to compare and identify differential proteins from N.flagelliforme subjected to desiccation.The results indicated that Ferritin,Dps family protein expression quantum gradually declined under desiccation stress.The Ferritin gene was cloned by designed degeneracy primer based on identified amino acid sequences.A full length of 540 bp DNA was obtained(GenBank access number: HM854287).Homology analysis showed that the N.flagelliforme Ferritin gene had high consensus regions.The secondary structures of Ferritin were made up of α helix and random coil.The RT-PCR showed that mRNA of Ferritin gradually declined under drought stress.The Ferritin gene was expressed in Escherichia coli,and a 22.4 kD heterologous protein was observed.The results laid a foundation for understanding the drought-resistant molecular mechanisms and protection mechanisms of N.flagelliforme in extreme arid environments.
鞭毛藻是一种陆地固氮蓝藻,具有较强的干旱适应性。我们采用了2-DE分析、凝胶图像分析和MALDI-TOF-TOF/MS来比较和鉴定干燥条件下的N.flagelliforme的差异蛋白。结果表明,在干燥胁迫下,铁蛋白、Dps家族蛋白的表达量逐渐下降。利用设计的退化引物,根据鉴定的氨基酸序列克隆铁蛋白基因。获得全长540 bp的DNA (GenBank访问号:HM854287)。同源性分析表明,N.flagelliforme铁蛋白基因具有较高的一致性区域。铁蛋白二级结构由α螺旋和无规螺旋组成。RT-PCR结果显示,干旱胁迫下,铁蛋白mRNA逐渐下降。铁蛋白基因在大肠杆菌中表达,得到22.4 kD的异源蛋白。研究结果为进一步了解极端干旱环境下N.flagelliforme的抗旱分子机制和保护机制奠定了基础。
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引用次数: 0
Analysis of Genetic Diversity of Jute(Corchorus L.) Germplasm Revealed by SRAP 黄麻遗传多样性分析SRAP揭示的种质资源
Pub Date : 2012-01-01 DOI: 10.3724/SP.J.1142.2012.20178
A. Tao, J. Qi, Jian-guang Su, Pingping Fang, Lihui Lin, Jiantang Xu, Wu Jianmei, P. Lin
{"title":"Analysis of Genetic Diversity of Jute(Corchorus L.) Germplasm Revealed by SRAP","authors":"A. Tao, J. Qi, Jian-guang Su, Pingping Fang, Lihui Lin, Jiantang Xu, Wu Jianmei, P. Lin","doi":"10.3724/SP.J.1142.2012.20178","DOIUrl":"https://doi.org/10.3724/SP.J.1142.2012.20178","url":null,"abstract":"","PeriodicalId":20134,"journal":{"name":"Plant Science Journal","volume":"30 1","pages":"178"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69786220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of High Temperature Stress on Electrical Impedance Spectroscopy Parameters of Chrysanthemum 高温胁迫对菊花电阻抗谱参数的影响
Pub Date : 2012-01-01 DOI: 10.3724/SP.J.1142.2012.20198
Yi-chao Liu, Jianfu Xiao, Ji-shuang Yang
To study the heat tolerance of chrysanthemum(Chrysanthemum morifolium),the electrical impedance spectroscopy(EIS) parameters of chrysanthemum'Jinba'stems and leaves under high temperature stress(25℃ to 50℃) were measured,and the relationship between electrolyte leakage(relative conductivity) and EIS parameters was analyzed.The results showed that extracellular resistance,intracellular resistance,and relaxation time of stems and leaves first increased and then decreased significantly with increasing temperature.However,the trends of distribution coefficient of relaxation time of stems and leaves showed irregular decrease under high temperature stress.The relative conductivity of chrysanthmum increased,then decreased,and finally increased significantly.Heat tolerance of chrysanthemum estimated by EIS parameters was close to that assessed by the EL method.Significant correlation between relative conductivity and extracellular resistance(stem: p0.01,leaf p0.05) and relaxation time(p0.05) of stem and leaf were found.In conclusion,extracellular resistance and relaxation time could be suitable parameters for determining heat tolerance of chrysanthemum.
为研究菊花(chrysanthemum morifolium)的耐热性,测定了高温胁迫(25℃~ 50℃)下菊花金巴茎叶的电阻抗谱(EIS)参数,并分析了电解质泄漏(相对电导率)与EIS参数的关系。结果表明:随着温度的升高,茎叶的胞外阻力、胞内阻力和松弛时间均呈先升高后降低的趋势;而茎叶松弛时间分配系数在高温胁迫下呈不规则下降趋势。菊花的相对电导率先升高后降低,最后显著升高。用EIS法测定的菊花耐热性与用EL法测定的结果接近。茎、叶的相对电导率与细胞外电阻(茎:p0.01,叶:p0.05)和松弛时间(茎、叶:p0.05)呈极显著相关。综上所述,细胞外抗性和松弛时间可作为测定菊花耐热性的适宜参数。
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引用次数: 1
Understanding Population Dynamics of Euptelea pleiospermum from Spatial Distribution Characteristics 从空间分布特征看多胚欧洲菜种群动态
Pub Date : 2012-01-01 DOI: 10.3724/SP.J.1142.2012.30213
Dong He, M. Jiang
Euptelea pleiospermum,a Tertiary relic and East-Asian endemic species,is a rare and threatened species in China,which requires protection.Based on stem mapping data,the spatial characteristics of E.pleiospermum in the Shennongjia Mountains were examined by nearest neighbor distance statistics.Following the pattern differences and changes within and between each life-history stage(represented by size class,seedling ≤2.5 cm,2.5 cmsapling≤7.5 cm,adult tree7.5 cm in DBH),the pattern-forming processes such as seedling establishment,recruitment and intra-specific competition were inferred and assessed.Euptelea pleiospermum was overall aggregated at the neighborhood scale.Sizes of small individuals(seedlings or saplings) showed no correlation with distances to nearest larger individuals(saplings or adult trees).Within a given radius of focal large-size individuals(saplings or adult trees),the probability of occurrence of size-identical and smaller individuals(seedlings or saplings) were approximately equal.Mean nearest neighbor distance from large-size individuals to nearest individuals in consecutive lower class(sapling vs.nearest seedling,or adult tree vs.nearest sapling) did not differ significantly from that measure within large size classes(within sapling or adult tree classes).Both summed sizes of nearest neighbor pairs and sizes of focal individuals were significantly correlated with nearest neighbor distances.Mean nearest neighbor distance within sapling class did not differ from the expected one within random-mortality-introduced seedling class,and likewise mean nearest neighbor distance within adult class did not differ from the expected one within random-mortality-introduced juvenile class(seedlings+saplings).These results suggest that(1) the aggregated distribution of E.pleiospermum may be due to the effects of seed dispersal and/or habitat heterogeneity on seedling establishment,(2) within their neighborhood established large individuals of E.pleiospermum did not appear to limit the locations and growth of smaller con-specific individuals,but neighbor-interactions may have an effect on long-term recruitment,(3) intra-specific competition existed in the E.pleiospermum population but it was not strong enough to produce distance-dependent mortality.
pleuptelea pleiospermum是中国第三纪遗存和东亚特有种,是一种珍稀濒危物种,需要保护。基于茎图资料,采用最近邻距离统计方法研究了神农架地区多乳草的空间分布特征。根据各生活史阶段(以大小类别、幼苗≤2.5 cm、2.5 cm树苗≤7.5 cm、成树胸径7.5 cm为代表)内模式的差异和变化,推断和评价了树苗建立、招募和种内竞争等模式形成过程。以邻域尺度总体聚集。小个体(幼苗或树苗)的大小与最近的大个体(树苗或成年树)的距离没有相关性。在一定的焦点大尺寸个体(树苗或成树)半径内,大小相同的个体和较小的个体(树苗或树苗)出现的概率大致相等。在连续的低等级(幼树与最近的幼苗,或成树与最近的幼树)中,大个体到最近个体的平均最近距离与在大等级(幼树或成树等级)中测量的结果没有显著差异。最近邻居对的总大小和焦点个体的大小都与最近邻居距离显著相关。树苗类内的平均近邻距离与随机死亡率引种苗类内的期望距离无显著差异,成虫类内的平均近邻距离与随机死亡率引种苗类(幼苗+树苗)内的期望距离无显著差异。结果表明:(1)多胚的聚集分布可能是由于种子传播和/或生境异质性对幼苗建立的影响;(2)多胚在其邻近区域内建立的大个体似乎不会限制小的同种个体的位置和生长,但邻居相互作用可能对长期招募有影响;(3)多胚种群中存在种内竞争,但不存在种内竞争强到足以产生与距离相关的死亡率。
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引用次数: 0
Preparation and Structural Characterization of wPNP-a1 fromPholiota nameko 凤仙花wPNP-a1的制备及结构表征
Pub Date : 2012-01-01 DOI: 10.3724/SP.J.1142.2012.40423
Guo Guo, Run-guang Sun, Q. Guo, Xiao-fei Zhang, Pei-Juan Dou
{"title":"Preparation and Structural Characterization of wPNP-a1 fromPholiota nameko","authors":"Guo Guo, Run-guang Sun, Q. Guo, Xiao-fei Zhang, Pei-Juan Dou","doi":"10.3724/SP.J.1142.2012.40423","DOIUrl":"https://doi.org/10.3724/SP.J.1142.2012.40423","url":null,"abstract":"","PeriodicalId":20134,"journal":{"name":"Plant Science Journal","volume":"30 1","pages":"423"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69786785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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Plant Science Journal
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