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Different Response Mechanisms of Rhizosphere Microbial Communities in Two Species of Amorphophallus to Pectobacterium carotovorum subsp. carotovorum Infection. 两种魔芋根际微生物群落对胡萝卜乳杆菌的不同响应机制carotovorum感染。
IF 2.3 3区 农林科学 Q2 PLANT SCIENCES Pub Date : 2023-04-01 DOI: 10.5423/PPJ.OA.12.2022.0157
Min Yang, Ying Qi, Jiani Liu, Penghua Gao, Feiyan Huang, Lei Yu, Hairu Chen

Soft rot is a widespread, catastrophic disease caused by Pectobacterium carotovorum subsp. carotovorum (Pcc) that severely damages the production of Amorphophallus spp. This study evaluated the rhizosphere bacterial and fungal communities in Pcc-infected and uninfected plants of two species of Amorphophallus, A. muelleri and A. konjac. Principal component analysis showed that the samples formed different clusters according to the Pcc infection status, indicating that Pcc infection can cause a large number of changes in the bacterial and fungal communities in the Amorphophallus spp. rhizosphere soil. However, the response mechanisms of A. muelleri and A. konjac are different. There was little difference in the overall microbial species composition among the four treatments, but the relative abundances of core microbiome members were significantly different. The relative abundances of Actinobacteria, Chloroflexi, Acidobacteria, Firmicutes, Bacillus, and Lysobacter were lower in infected A. konjac plants than in healthy plants; in contrast, those of infected A. muelleri plants were higher than those in healthy plants. For fungi, the relative abundances of Ascomycota and Fusarium in the rhizosphere of infected A. konjac plants were significantly higher than those of healthy plants, but those of infected A. muelleri plants were lower than those of healthy plants. The relative abundance of beneficial Penicillium fungi was lower in infected A. konjac plants than in healthy plants, and that of infected A. muelleri plants was higher than that of healthy plants. These findings can provide theoretical references for further functional research and utilization of Amorphophallus spp. rhizosphere microbial communities in the future.

软腐病是由胡萝卜乳杆菌亚种引起的一种广泛的灾难性疾病。本研究以魔芋属植物muelleri和魔芋属植物为研究对象,对其侵染和未侵染的根际细菌和真菌群落进行了研究。主成分分析表明,不同Pcc感染程度的样品形成不同的聚类,说明Pcc感染会引起魔芋根际土壤细菌和真菌群落的大量变化。然而,木芋和魔芋的反应机制不同。4个处理间总体微生物种类组成差异不大,但核心微生物组成员相对丰度差异显著。放线菌门、绿杆菌门、酸杆菌门、厚壁菌门、芽孢杆菌门和溶菌门的相对丰度在感染魔芋植株中低于健康植株;与此相反,染病的木耳植株的叶绿素含量高于健康植株。真菌方面,侵染魔芋根际子囊菌群和镰刀菌群的相对丰度显著高于健康植株,而侵染魔芋根际子囊菌群和镰刀菌群的相对丰度低于健康植株。染病魔芋植株中有益青霉菌的相对丰度低于健康植株,染病魔芋植株中有益青霉菌的相对丰度高于健康植株。这些发现可为今后魔芋根际微生物群落功能的进一步研究和利用提供理论参考。
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引用次数: 0
Regulation of Salicylic Acid and N-Hydroxy-Pipecolic Acid in Systemic Acquired Resistance. 水杨酸和n -羟基胡椒酸在全身获得性耐药中的调控作用。
IF 2.3 3区 农林科学 Q2 PLANT SCIENCES Pub Date : 2023-02-01 DOI: 10.5423/PPJ.RW.10.2022.0145
Gah-Hyun Lim

In plants, salicylic acid (SA) is a central immune signal that is involved in both local and systemic acquired resistance (SAR). In addition to SA, several other chemical signals are also involved in SAR and these include N-hydroxy-pipecolic acid (NHP), a newly discovered plant metabolite that plays a crucial role in SAR. Recent discoveries have led to a better understanding of the biosynthesis of SA and NHP and their signaling during plant defense responses. Here, I review the recent progress in role of SA and NHP in SAR. In addition, I discuss how these signals cooperate with other SAR-inducing chemicals to regulate SAR.

在植物中,水杨酸(SA)是一种中枢免疫信号,参与了局部和全身获得性抗性(SAR)。除了SA外,其他几种化学信号也参与SAR,其中包括n -羟基-黑果酸(NHP),这是一种新发现的植物代谢物,在SAR中起着至关重要的作用。最近的发现使人们更好地了解SA和NHP的生物合成及其在植物防御反应中的信号传导。在此,我综述了SA和NHP在SAR中的作用的最新进展,并讨论了这些信号如何与其他诱导SAR的化学物质协同调节SAR。
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引用次数: 1
Disruption of the metC Gene Affects Methionine Biosynthesis in Pectobacterium carotovorum subsp. carotovorum Pcc21 and Reduces Soft-Rot Disease. mec基因破坏对胡萝卜乳杆菌甲硫氨酸合成的影响。胡萝卜素Pcc21和减少软腐病。
IF 2.3 3区 农林科学 Q2 PLANT SCIENCES Pub Date : 2023-02-01 DOI: 10.5423/PPJ.OA.09.2022.0135
Seonmi Yu, Jihee Kang, Eui-Hwan Chung, Yunho Lee

Plant pathogenic Pectobacterium species cause severe soft rot/blackleg diseases in many economically important crops worldwide. Pectobacterium utilizes plant cell wall degrading enzymes (PCWDEs) as the main virulence determinants for its pathogenicity. In this study, we screened a random mutant, M29 is a transposon insertion mutation in the metC gene encoding cystathionine β-lyase that catalyzes cystathionine to homocysteine at the penultimate step in methionine biosynthesis. M29 became a methionine auxotroph and resulted in growth defects in methionine-limited conditions. Impaired growth was restored with exogenous methionine or homocysteine rather than cystathionine. The mutant exhibited reduced soft rot symptoms in Chinese cabbages and potato tubers, maintaining activities of PCWDEs and swimming motility. The mutant was unable to proliferate in both Chinese cabbages and potato tubers. The reduced virulence was partially restored by a complemented strain or 100 µM of methionine, whereas it was fully restored by the extremely high concentration (1 mM). Our transcriptomic analysis showed that genes involved in methionine biosynthesis or transporter were downregulated in the mutant. Our results demonstrate that MetC is important for methionine biosynthesis and transporter and influences its virulence through Pcc21 multiplication in plant hosts.

植物致病性胸腺杆菌在世界上许多重要的经济作物中引起严重的软腐病/黑腿病。乳杆菌利用植物细胞壁降解酶(PCWDEs)作为其致病性的主要毒力决定因素。在这项研究中,我们筛选了一个随机突变,M29是编码半胱氨酸β-裂解酶的metC基因的转座子插入突变,该酶在蛋氨酸生物合成的倒数第二步催化半胱氨酸转化为同型半胱氨酸。M29成为蛋氨酸营养不良者,在蛋氨酸限制条件下导致生长缺陷。用外源蛋氨酸或同型半胱氨酸而不是半胱硫氨酸恢复受损的生长。该突变体在白菜和马铃薯块茎中表现出较弱的软腐病症状,保持了PCWDEs的活性和游泳运动。该突变体不能在白菜和马铃薯块茎中增殖。补充菌株或100µM蛋氨酸可部分恢复原毒力,而极高浓度(1 mM)可完全恢复原毒力。我们的转录组学分析显示,参与蛋氨酸生物合成或转运的基因在突变体中下调。我们的研究结果表明,MetC是蛋氨酸生物合成和转运的重要载体,并通过Pcc21在植物宿主中的增殖影响其毒力。
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引用次数: 1
Past and Future Epidemiological Perspectives and Integrated Management of Rice Bakanae in Korea. 韩国水稻白斑病的流行病学研究与综合治理。
IF 2.3 3区 农林科学 Q2 PLANT SCIENCES Pub Date : 2023-02-01 DOI: 10.5423/PPJ.RW.08.2022.0123
Soobin Shin, Hyunjoo Ryu, Jin-Yong Jung, Yoon-Ju Yoon, Gudam Kwon, Nahyun Lee, Na Hee Kim, Rowoon Lee, Jiseon Oh, Minju Baek, Yoon Soo Choi, Jungho Lee, Kwang-Hyung Kim

In the past, rice bakanae was considered an endemic disease that did not cause significant losses in Korea; however, the disease has recently become a serious threat due to climate change, changes in farming practices, and the emergence of fungicide-resistant strains. Since the bakanae outbreak in 2006, its incidence has gradually decreased due to the application of effective control measures such as hot water immersion methods and seed disinfectants. However, in 2013, a marked increase in bakanae incidence was observed, causing problems for rice farmers. Therefore, in this review, we present the potential risks from climate change based on an epidemiological understanding of the pathogen, host plant, and environment, which are the key elements influencing the incidence of bakanae. In addition, disease management options to reduce the disease pressure of bakanae below the economic threshold level are investigated, with a specific focus on resistant varieties, as well as chemical, biological, cultural, and physical control methods. Lastly, as more effective countermeasures to bakanae, we propose an integrated disease management option that combines different control methods, including advanced imaging technologies such as remote sensing. In this review, we revisit and examine bakanae, a traditional seed-borne fungal disease that has not gained considerable attention in the agricultural history of Korea. Based on the understanding of the present significance and anticipated risks of the disease, the findings of this study are expected to provide useful information for the establishment of an effective response strategy to bakanae in the era of climate change.

过去,水稻白斑病被认为是一种地方病,在韩国不会造成重大损失;然而,由于气候变化、耕作方式的改变以及抗杀菌剂菌株的出现,这种疾病最近已成为一种严重威胁。自2006年巴卡纳暴发以来,由于采用了热水浸泡法和种子消毒剂等有效控制措施,其发病率已逐渐下降。然而,在2013年,观察到bakanae发病率显著增加,给水稻农民带来了问题。因此,本文在对影响bakanae发病的病原、寄主植物和环境的流行病学认识的基础上,提出了气候变化的潜在风险。此外,还研究了将bakanae的疾病压力降低到经济阈值以下的疾病管理方案,特别侧重于抗性品种,以及化学、生物、文化和物理控制方法。最后,作为更有效的对策,我们提出了一种综合疾病管理方案,结合不同的控制方法,包括先进的成像技术,如遥感。在这篇综述中,我们重新审视和研究bakanae,一种传统的种子传播的真菌疾病,在韩国的农业历史上没有得到相当大的关注。基于对该疾病的当前意义和预期风险的理解,本研究的结果有望为气候变化时代建立有效的应对策略提供有用的信息。
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引用次数: 0
The Crucial Role of Chloroplast-Related Proteins in Viral Genome Replication and Host Defense against Positive-Sense Single-Stranded RNA Viruses. 叶绿体相关蛋白在病毒基因组复制和宿主防御正义单链RNA病毒中的关键作用。
IF 2.3 3区 农林科学 Q2 PLANT SCIENCES Pub Date : 2023-02-01 DOI: 10.5423/PPJ.RW.10.2022.0139
John Bwalya, Kook-Hyung Kim

Plant viruses are responsible for worldwide production losses of numerous economically important crops. The most common plant RNA viruses are positivesense single-stranded RNA viruses [(+)ss RNA viruses]. These viruses have small genomes that encode a limited number of proteins. The viruses depend on their host's machinery for the replication of their RNA genome, assembly, movement, and attraction to the vectors for dispersal. Recently researchers have reported that chloroplast proteins are crucial for replicating (+)ss plant RNA viruses. Some chloroplast proteins, including translation initiation factor [eIF(iso)4E] and 75 DEAD-box RNA helicase RH8, help viruses fulfill their infection cycle in plants. In contrast, other chloroplast proteins such as PAP2.1, PSaC, and ATPsyn-α play active roles in plant defense against viruses. This is also consistent with the idea that reactive oxygen species, salicylic acid, jasmonic acid, and abscisic acid are produced in chloroplast. However, knowledge of molecular mechanisms and functions underlying these chloroplast host factors during the virus infection is still scarce and remains largely unknown. Our review briefly summarizes the latest knowledge regarding the possible role of chloroplast in plant virus replication, emphasizing chloroplast-related proteins. We have highlighted current advances regarding chloroplast-related proteins' role in replicating plant (+)ss RNA viruses.

植物病毒对世界范围内许多重要经济作物的生产损失负有责任。最常见的植物RNA病毒是正义单链RNA病毒[(+)ss RNA病毒]。这些病毒的基因组很小,只能编码有限数量的蛋白质。病毒依靠宿主的机制进行RNA基因组的复制、组装、移动和吸引载体进行传播。最近研究人员报道了叶绿体蛋白在复制(+)ss植物RNA病毒中起着至关重要的作用。一些叶绿体蛋白,包括翻译起始因子[eIF(iso)4E]和75 DEAD-box RNA解旋酶RH8,帮助病毒在植物中完成感染周期。相比之下,其他叶绿体蛋白如PAP2.1、PSaC和ATPsyn-α在植物防御病毒中发挥积极作用。这也与活性氧、水杨酸、茉莉酸和脱落酸是在叶绿体中产生的观点一致。然而,关于这些叶绿体宿主因子在病毒感染过程中的分子机制和功能的知识仍然很少,而且在很大程度上仍然是未知的。本文简要总结了叶绿体在植物病毒复制中可能发挥的作用,重点介绍了叶绿体相关蛋白的研究进展。我们重点介绍了叶绿体相关蛋白在复制植物(+)ss RNA病毒中的作用的最新进展。
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引用次数: 1
Colony Age of Trichoderma azevedoi Alters the Profile of Volatile Organic Compounds and Ability to Suppress Sclerotinia sclerotiorum in Bean Plants. 毛霉菌的菌落年龄会改变挥发性有机化合物的分布以及抑制豆类植物中硬皮病菌的能力。
IF 1.8 3区 农林科学 Q2 PLANT SCIENCES Pub Date : 2023-02-01 DOI: 10.5423/PPJ.OA.08.2022.0106
Lincon Rafael da Silva, Leonardo Luís de Barros Rodrigues, Amanda Silva Botelho, Bruna Sartório de Castro, Paulo Henrique Pereira Costa Muniz, Maria Carolina Blassioli Moraes, Sueli Corrêa Marques de Mello

Common bean (Phaseolus vulgaris L.) is one of the most important crops in human food production. The occurrence of diseases, such as white mold, caused by Sclerotinia sclerotiorum can limit the production of this legume. The use of Trichoderma has become an important strategy in the suppression of this disease. The aim of the present study was to evaluate the effect of volatile organic compounds (VOCs) emitted by Trichoderma azevedoi CEN1241 in five different growth periods on the severity of white mold in common bean. The in vitro assays were carried out in double-plate and split-plate, and the in vivo assays, through the exposure of the mycelia of S. sclerotiorum to the VOCs of T. azevedoi CEN1241 and subsequent inoculation in bean plants. Chemical analysis by gas chromatography coupled to mass spectrometry detected 37 VOCs produced by T. azevedoi CEN1241, covering six major chemical classes. The profile of VOCs produced by T. azevedoi CEN1241 varied according to colony age and was shown to be related to the ability of the biocontrol agent to suppress S. sclerotiorum. T. azevedoi CEN1241 VOCs reduced the size of S. sclerotiorum lesions on bean fragments in vitro and reduced disease severity in a greenhouse. This study demonstrated in a more applied way that the mechanism of antibiosis through the production of volatile compounds exerted by Trichoderma can complement other mechanisms, such as parasitism and competition, thus contributing to a better efficiency in the control of white mold in bean plants.

蚕豆(Phaseolus vulgaris L.)是人类粮食生产中最重要的作物之一。由硬粒菌(Sclerotinia sclerotiorum)引起的白霉等病害的发生会限制这种豆科植物的产量。使用毛霉菌已成为抑制这种病害的重要策略。本研究旨在评估 Trichoderma azevedoi CEN1241 在五个不同生长期释放的挥发性有机化合物(VOCs)对蚕豆白霉严重程度的影响。体外试验采用双板和分板法进行,体内试验则是将硬毛菌的菌丝体暴露于 T. azevedoi CEN1241 的挥发性有机化合物中,然后接种到蚕豆植株中。通过气相色谱-质谱联用技术进行的化学分析检测出 T. azevedoi CEN1241 产生的 37 种挥发性有机化合物,涵盖六大类化学物质。T. azevedoi CEN1241 产生的挥发性有机化合物随菌落年龄的不同而变化,并被证明与生物控制剂抑制硬核菌的能力有关。T. azevedoi CEN1241 的挥发性有机化合物能在体外缩小豆角硬皮病病斑的大小,并在温室中减轻病害的严重程度。这项研究以一种更实用的方式证明,通过毛霉产生的挥发性化合物进行抗菌的机制可以补充其他机制,如寄生和竞争机制,从而有助于更有效地控制豆科植物的白霉病。
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引用次数: 0
Application of Rapid and Reliable Detection of Cymbidium Mosaic Virus by Reverse Transcription Recombinase Polymerase Amplification Combined with Lateral Flow Immunoassay. 逆转录重组酶聚合酶扩增联合横向流动免疫法快速可靠检测蕙兰花叶病毒的应用
IF 2.3 3区 农林科学 Q2 PLANT SCIENCES Pub Date : 2023-02-01 DOI: 10.5423/PPJ.ER.10.2022.0147
Do-Hyun Kim, Rae-Dong Jeong, Sena Choi, Ho-Jong Ju, Ju-Yeon Yoon
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引用次数: 0
Development of the Droplet Digital PCR Method for the Detection and Quantification of Erwinia pyrifoliae. 液滴数字PCR检测与定量方法的建立。
IF 2.3 3区 农林科学 Q2 PLANT SCIENCES Pub Date : 2023-02-01 DOI: 10.5423/PPJ.NT.08.2022.0117
Lin He, Seong Hwan Kim, Jun Myoung Yu

Black shoot blight disease caused by Erwinia pyrifoliae has serious impacts on quality and yield in pear production in Korea; therefore, rapid and accurate methods for its detection are needed. However, traditional detection methods require a great deal of time and fail to achieve absolute quantification. In the present study, we developed a droplet digital polymerase chain reaction (ddPCR) method for the detection and absolute quantification of E. pyrifoliae using a pair of species-specific primers. The detection range was 103 - 107 copies/ml (DNA templates) and cfu/ml (cell culture templates). This new method exhibited good linearity and repeatability and was validated by absolute quantification of E. pyrifoliae DNA copies from samples of artificially inoculated immature pear fruits. Here, we present the first study of ddPCR assay for the detection and quantification of E. pyrifoliae. This method has potential applications in epidemiology and for the early prediction of black shoot blight outbreaks.

梨黑梢疫病严重影响韩国梨的品质和产量。因此,需要快速准确的检测方法。然而,传统的检测方法需要耗费大量的时间,且无法做到绝对定量。在本研究中,我们建立了一种液滴数字聚合酶链反应(ddPCR)方法,利用一对物种特异性引物检测和绝对定量pyrifoliae。检测范围为103 ~ 107拷贝/ml (DNA模板)和cfu/ml(细胞培养模板)。该方法具有良好的线性和重复性,并通过对人工接种的梨幼果样品进行绝对定量验证。在这里,我们提出了第一个ddPCR检测和定量的研究。该方法在流行病学和疫病的早期预测中具有潜在的应用价值。
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引用次数: 0
Anti-Oomycete Activity and Pepper Root Colonization of Pseudomonas plecoglossicida YJR13 and Pseudomonas putida YJR92 against Phytophthora capsici. 紫舌假单胞菌YJR13和腐臭假单胞菌YJR92抗辣椒疫霉菌活性及辣椒根定殖
IF 2.3 3区 农林科学 Q2 PLANT SCIENCES Pub Date : 2023-02-01 DOI: 10.5423/PPJ.OA.01.2023.0001
Elena Volynchikova, Ki Deok Kim

Previously, Pseudomonas plecoglossicida YJR13 and Pseudomonas putida YJR92 from a sequential screening procedure were proven to effectively control Phytophthora blight caused by Phytophthora capsici. In this study, we further investigated the anti-oomycete activities of these strains against mycelial growth, zoospore germination, and germ tube elongation of P. capsici. We also investigated root colonization ability of the bacterial strains in square dishes, including cell motility (swimming and swarming motilities) and biofilm formation. Both strains significantly inhibited mycelial growth in liquid and solid V8 juice media and M9 minimal media, zoospore germination, and germ tube elongation compared with Bacillus vallismortis EXTN-1 (positive biocontrol strain), Sphingomonas aquatilis KU408 (negative biocontrol strain), and MgSO4 solution (untreated control). In diluted (nutrient-deficient) V8 juice broth, the tested strain populations were maintained at >108 cells/ml, simultaneously providing mycelial inhibitory activity. Additionally, these strains colonized pepper roots at a 106 cells/ml concentration for 7 days. The root colonization of the strains was supported by strong swimming and swarming activities, biofilm formation, and chemotactic activity towards exudate components (amino acids, organic acids, and sugars) of pepper roots. Collectively, these results suggest that strains YJR13 and YJR92 can effectively suppress Phytophthora blight of pepper through direct anti-oomycete activities against mycelial growth, zoospore germination and germ tube elongation. Bacterial colonization of pepper roots may be mediated by cell motility and biofilm formation together with chemotaxis to root exudates.

此前,通过序筛选,plecogssidida YJR13和Pseudomonas putida YJR92被证明能有效控制辣椒疫霉引起的疫病。在本研究中,我们进一步研究了这些菌株对辣椒粉菌丝生长、游动孢子萌发和胚管伸长的抑制活性。我们还研究了菌株在方形培养皿中的根定植能力,包括细胞运动(游泳和群体运动)和生物膜形成。两株菌株在液体和固体V8果汁培养基和M9培养基中,与芽孢杆菌(Bacillus vallismortis) EXTN-1(阳性生防菌株)、水鞘单胞菌(Sphingomonas aquatilis KU408(阴性生防菌株)和MgSO4溶液(未处理对照)相比,均显著抑制菌丝生长、虫孢子萌发和芽管伸长。在稀释的(营养缺乏的)V8果汁肉汤中,所测试的菌株数量保持在>108个细胞/ml,同时提供菌丝抑制活性。此外,这些菌株以106个细胞/ml的浓度在辣椒根部定植7天。菌株的根定植受到强大的游动和群体活动、生物膜形成以及对辣椒根系渗出成分(氨基酸、有机酸和糖)的趋化活性的支持。综上所述,菌株YJR13和YJR92可以通过直接抑制卵菌对辣椒菌丝生长、虫孢子萌发和胚管伸长的抑制作用,有效抑制辣椒疫霉病。细菌在辣椒根中的定植可能通过细胞运动、生物膜的形成以及对根分泌物的趋化性来介导。
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引用次数: 1
Molecular Detection of Phytoplasmas of the 16SrⅠ and 16SrXXXⅡ Groups in Elaeocarpus sylvestris Trees with Decline Disease in Jeju Island, South Korea. 济州岛Elaeocarpus sylvestris病树16SrⅠ和16SrXXXⅡ群植物原体的分子检测
IF 2.3 3区 农林科学 Q2 PLANT SCIENCES Pub Date : 2023-02-01 DOI: 10.5423/PPJ.OA.07.2022.0091
Geon-Woo Lee, Sang-Sub Han

Phytoplasmas were discovered in diseased Elaeocarpus sylvestris trees growing on Jeju Island that showed symptoms of yellowing and darkening in the leaves. Leaf samples from 14 symptomatic plants in Jeju-si and Seogwipo-si were collected and phytoplasma 16S rRNA was successfully amplified by nested polymerase chain reaction using universal primers. The sequence analysis detected two phytoplasmas, which showed 99.5% identity to 'Candidatus Phytoplasma asteris' and 'Ca. P. malaysianum' affiliated to 16SrI and 16SrXXXII groups, respectively. Through polymerase chain reaction-restriction fragment length polymorphism (RFLP) analyses using the AfaI (RsaI) restriction enzyme, the presence of two phytoplasmas strains as well as cases of mixed infection of these strains was detected. In a virtual RFLP analysis with 17 restriction enzymes, the 16S rRNA sequence of the 'Ca. P. asteris' strain was found to match the pattern of the 16SrI-B subgroup. In addition, the phytoplasmas in the mixed-infection cases could be distinguished using specific primer sets. In conclusion, this study confirmed mixed infection of two phytoplasmas in one E. sylvestris plant, and also the presence of two phytoplasmas (of the 16SrⅠ and 16SrXXXⅡ groups) in Jeju Island (Republic of Korea).

在济州岛生长的病树中发现了植物原体,叶子变黄变黑。收集济州和西归浦地区14株病状植物的叶片样本,利用通用引物巢式聚合酶链反应成功扩增出植物原体16S rRNA。序列分析结果显示,2个植物原体分别属于16SrI和16SrXXXII类群的‘Candidatus Phytoplasma asteris’和‘Ca. P. malaysium’,同源性达99.5%。采用AfaI (RsaI)限制性内切酶进行聚合酶链反应-限制性片段长度多态性(RFLP)分析,检测到两株植物原体菌株的存在以及两株植物原体混合感染的病例。通过17种限制性内切酶的虚拟RFLP分析,发现‘Ca. P. asteris’菌株的16S rRNA序列与16SrI-B亚群的模式相匹配。此外,混合感染病例中的植物原体可以通过特异性引物进行区分。结果表明,在韩国济州岛,一株西尔维斯特植物中存在两种植物原体的混合侵染,并且存在两种植物原体(16SrⅠ和16SrXXXⅡ群)。
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引用次数: 0
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