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FLUORESCENCE STUDIES ON THE BLOOD PLASMA ALBUMIN INTERACTION WITH 4-HYDROXY-2-METHYLQUINOLINE 血浆白蛋白与4-羟基-2-甲基喹啉相互作用的荧光研究
Pub Date : 2022-06-30 DOI: 10.46991/pysu:b/2022.56.2.100
K. Grigoryan, H. Shilajyan, I. Aleksanyan, L. Hambardzumyan, Hasmik H. Hovhannisyan
Blood plasma albumin (bovine serum albumin, BSA) interaction with a biologically active reagent 4-hydroxy-2-methylquinoline (4H2MQ) has been studied using fluorescence spectroscopy methods (steady state, synchronous, excitation/emission matrix, 3D spectra). With the addition of 4H2MQ, no changes in the spectral characteristics of BSA (fluorescence emission intensity and λmax position) are observed; only the symmetry of the BSA emission spectrum is changed and the full width at half maximum is decreased. The analysis of synchronous and 3D fluorescence spectra showed the existence of three emitting species in this system, which could be a result of changes in the fluorescence and photochemical properties of 4H2MQ.
利用荧光光谱方法(稳态、同步、激发/发射矩阵、三维光谱)研究了血浆白蛋白(牛血清白蛋白,BSA)与生物活性试剂4-羟基-2-甲基喹啉(4H2MQ)的相互作用。添加4H2MQ后,BSA的光谱特性(荧光发射强度和λmax位置)没有变化;仅改变了BSA发射光谱的对称性,减小了半最大值时的全宽度。同步和三维荧光光谱分析表明,该体系中存在三种发光物质,这可能是由于4H2MQ的荧光和光化学性质发生了变化。
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引用次数: 0
NEW TYPES OF FLAME RETARDANTS BASED ON PHOSPHATE DERIVATIVES 基于磷酸盐衍生物的新型阻燃剂
Pub Date : 2022-06-30 DOI: 10.46991/pysu:b/2022.56.2.131
M. L. Yeritsyan, Armen M. Arustamyan, I. N. Sirekanyan, Lena G. Mkoyan, Lilit Stepanyan
One of the main problems of nature conservation and protection of the environ-ment is the reducing of flammability of natural and synthetic materials as well as products based on them. In order to obtain non-combustible materials such as flame retardants, monosodium salt of orthophosphoric acid, urea, thiourea and their mono-methylol derivatives, as well as N-monomethylol acrylamide were used in this work. The reaction for the synthesis of the mentioned compounds was carried out in the presence of a small amount of water. During the reaction between urea and monosodium salt of orthophosphoric acid, ammonia and СО2 were released. In the case of the reaction of thiocarbamide with the monosodium salt of orthophosphoric acid, in addition to ammonia and CO2, hydrogen sulfide is partially released, the formation mechanism of which is presented in the work.
降低天然材料和合成材料及其制品的可燃性是自然保护和环境保护的主要问题之一。为了获得阻燃剂等不燃材料,本工作中使用了正磷酸钠盐、尿素、硫脲及其单甲基衍生物以及n-单甲基丙烯酰胺。上述化合物的合成反应是在少量水的存在下进行的。尿素与正磷酸单钠盐反应时,释放出氨和СО2。硫代氨基脲与正磷酸单钠盐反应时,除氨和CO2外,还部分释放出硫化氢,本文介绍了硫化氢的形成机理。
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引用次数: 0
THE INFLUENCE OF THE ACIDIC TREATMENT OF PEAT ON ITS SORPTION PROPERTIES 泥炭酸处理对其吸附性能的影响
Pub Date : 2022-06-30 DOI: 10.46991/pysu:b/2022.56.2.120
S. Hayrapetyan, L. S. Banyan, L. A. Margaryan, H. H. Khachatryan, A. I. Martiryan
The influence of the acidic treatment of peat on its sorption properties has been studied by means of optical emission spectrometry with inductively coupled plasma (ICP-OES) and pH-metry methods. Untreated peat of the Vardenis deposit, Armenia, and acid-activated peat were used as sorption materials. As an activation process, we consider preferably the elimination of metal ions captured on the peat surface. During this active sorption groups (particularly acetic) are released from the surface and such acidic groups together with other functional groups begin to act according to the cation exchange mechanism.
采用电感耦合等离子体发射光谱法(ICP-OES)和ph法研究了泥炭的酸性处理对其吸附性能的影响。采用亚美尼亚Vardenis矿床的未处理泥炭和酸活化泥炭作为吸附材料。作为一种活化过程,我们最好考虑去除泥炭表面捕获的金属离子。在这种活性吸附过程中,基团(尤其是乙酸)从表面释放出来,这些酸性基团与其他官能团一起开始根据阳离子交换机制起作用。
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引用次数: 0
STUDY OF STRUCTURAL TRANSITIONS OF COMPLEXES OF DIFFERENT LIGANDS WITH DNA AND BSA 不同配体与DNA和牛血清白蛋白配合物结构转变的研究
Pub Date : 2022-05-03 DOI: 10.46991/pysu:b/2022.56.1.049
P. Vardevanyan, Nara R. Petrosyan
Structural transitions of DNA and bovine serum albumin (BSA) and their complexes with methylene blue (MB) and Hoechst 33258 (H33258) by UV-denaturation method have been studied. The obtained results revel that MB stabilizes both protein dimensional structure and DNA double-stranded structure, while H33258 stabilizes double-stranded structure of DNA and destabilizes protein structure. It was also revealed that the stabilizing effect of MB on native structure of DNA is more pronounced at relatively low ligand concentrations, than on protein.
用紫外变性法研究了DNA与牛血清白蛋白(BSA)及其与亚甲基蓝(MB)和赫斯特33258 (H33258)配合物的结构转变。结果表明,MB具有稳定蛋白质维结构和DNA双链结构的作用,而H33258具有稳定DNA双链结构和破坏蛋白质结构的作用。研究还表明,相对较低的配体浓度下,MB对DNA天然结构的稳定作用比对蛋白质的稳定作用更明显。
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引用次数: 0
THE EFFECT OF BREASTFEEDING ON THE HEALTH INDICATORS OF YOUNG CHILDREN 母乳喂养对幼儿健康指标的影响
Pub Date : 2022-05-03 DOI: 10.46991/pysu:b/2022.56.1.084
S. Mkrtchyan, R. Dunamalyan, M. Mardiyan, L. Ghukasyan, G. Mirijanyan
Promotion and coverage of breastfeeding are considered to be the cost-effective public health measures in terms of Healthcare System. The aim of the research is to assess an influence of breastfeeding on infants’ health and quality of life (QL) parameters. This observational prospective study included 1790 newborns selected from Yerevan State Medical University’s Polyclinics (“Mouratsan”, sample=746, and “Heratsi”, sample=1044). Data collection took a period between January 2016 and December 2017. Evaluation of children’s quality of life was performed with the help of the validated and adapted QUALIN questionnaire. The data related to child’s health condition were collected from the “Child Development History” cards available at the polyclinics of the investigated area. Among 1770 one year old children 1681 were ever breastfed, 1224 (69.1%) were exclusively breastfed up to 3 months, the median duration of breastfeeding was 6–7 months, 45% were breastfed for more than 6 months, and 15% were breastfed for more than 1 year. There is a significant difference between the QL scores of the ever breastfed and the never breastfed subgroups. Duration of the breastfeeding also have an influence on QL scores. The given study’s results confirm that breastfeeding among the early aged Armenian children is of great importance in their health, influencing on quality of life and morbidity rates.
就卫生保健系统而言,促进和覆盖母乳喂养被认为是具有成本效益的公共卫生措施。研究的目的是评估母乳喂养对婴儿健康和生活质量(QL)参数的影响。这项观察性前瞻性研究包括从埃里温州立医科大学综合诊所挑选的1790名新生儿(“Mouratsan”,样本=746,“Heratsi”,样本=1044)。数据收集时间为2016年1月至2017年12月。儿童生活质量评价采用经验证和改编的QUALIN问卷进行。与儿童健康状况有关的数据是从调查地区综合诊所提供的"儿童发展史"卡片上收集的。1770名1岁儿童中,1681名曾经母乳喂养,1224名(69.1%)纯母乳喂养至3个月,母乳喂养中位时间为6 - 7个月,45%母乳喂养超过6个月,15%母乳喂养超过1年。曾经母乳喂养和从未母乳喂养亚组的QL评分有显著差异。母乳喂养的持续时间对QL评分也有影响。上述研究结果证实,亚美尼亚幼儿的母乳喂养对他们的健康、生活质量和发病率具有重要意义。
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引用次数: 0
AGARICOMYCETES MUSHROOMS DISTRIBUTED IN ARMENIA – SOURCE OF COSMETIC BIOINGRЕDIENTS 分布在亚美尼亚的菌类蘑菇-化妆品的来源bioingrЕdients
Pub Date : 2022-05-03 DOI: 10.46991/pysu:b/2022.56.1.074
S. Badalyan, Anush L. Barkhudaryan, N. Gharibyan
Currently, the cosmetic industry is in a constant search for valuable bioingredients with anti-aging (antioxidant, anti-inflammatory, anti-collagenase, anti-elastase, anti-hyaluronidase and anti-tyrosinase) properties. Edible and medicinal Agaricomycetes mushrooms (macrofungi) as unlimited source of bioactive compounds and enzymes have mainly been studied for their nutritional value and medicinal properties. They have also been included in cosmetic formulations for topical and oral administration however there is slow progress in their usage as cosmeceuticals.We described 19 medicinal, including 12 edible mushrooms belonging to 18 genera of Agaricomycetes fungi distributed in Armenia – as potential cosmeceuticals. Further evaluation of mushrooms resources in Armenia will revealed new species and promote their biotechnological exploitation not only as mushroom-derived nutraceuticals and pharmaceuticals, but also cosmeceuticals. Advances in biology and biotechnology of Agaricomycetes will further assist development of myco-cosmetology in Armenia.
目前,化妆品行业一直在寻找具有抗衰老(抗氧化、抗炎、抗胶原酶、抗弹性酶、抗透明质酸酶和抗酪氨酸酶)特性的有价值的生物成分。食用菌蘑菇(大型真菌)作为生物活性化合物和酶的无限来源,其营养价值和药用价值一直是人们研究的重点。它们也被包括在化妆品配方中,用于局部和口服给药,但它们作为药妆品的使用进展缓慢。我们描述了19种药用蘑菇,包括12种食用菌,隶属于分布在亚美尼亚的18属真菌,作为潜在的药妆品。对亚美尼亚蘑菇资源的进一步评估将发现新的菌种,并促进其生物技术开发,不仅可以作为蘑菇衍生的营养保健品和药品,还可以作为药妆品。菌丝体生物学和生物技术的进步将进一步促进亚美尼亚菌丝体美容的发展。
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引用次数: 0
THE ROLE OF MUSIC IN THE CORRECTION OF THE FUNCTIONAL STATE OF STUDENTS 音乐对学生功能状态的矫正作用
Pub Date : 2022-05-03 DOI: 10.46991/pysu:b/2022.56.1.094
E. Gevorkyan, L. Ghukasyan, K. V. Baghdasaryan
With the method of variational pulsometry by R.M.Baevsky, the influence of classical music on the integral and spectral characteristics of the activity of the regulatory mechanisms of the heart rhythm of students, observed during a one-day study load, was studied by the method of computer registration and analysis of the ECG. ECG registration and analysis were carried out on students of Yerevan State University in three experimental situations: before the first lesson (physiological norm); after the end of the 6th lesson; after listening to classical music for 20 min. Two types of response of the functional indicators of students to the study load were revealed: sympathetic and parasympathetic. It has been established that after a study load, a session of music therapy contributes to a return of the levels of all studied heart rate indicators towards the physiological norm (initial values before the class).
采用baevsky变分脉搏法,采用计算机对心电记录和分析的方法,研究了古典音乐对学生心律调节机制活动的积分特征和频谱特征的影响。对埃里温国立大学学生在三种实验情境下进行心电记录与分析:第一节课前(生理规范);第六节课结束后;实验结果显示,学生对学习负荷的功能指标有交感神经和副交感神经两种反应。已经确定,在学习负荷之后,一段时间的音乐治疗有助于将所有研究的心率指标水平恢复到生理标准(上课前的初始值)。
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引用次数: 0
THE FIRST RECORD OF THE GENUS $ARGIOPE$ OF THE FAMILY ARANEIDAE: $ARGIOPE$ $BRUENNICHI$ (SCOPOLI, 1772) AND $ARGIOPE$ $LOBATA$ (PALLAS, 1772) IN ARMENIA 亚美尼亚蜘蛛科$ argiope $ bruennichi $ (scopoli, 1772年)和$ argiope $ lobata $ (pallas, 1772年)的首次记录
Pub Date : 2022-05-03 DOI: 10.46991/pysu:b/2022.56.1.063
Armine Kosyan
The article presents the first record of the genus Argiope, orb-weaver spiders from the Araneidae family in Armenia. The two female specimens of Argiope bruennichi (Scopoli, 1772) were found in the Lori Region (northern part), and two specimens of the female spider of Argiope lobata (Pallas, 1772) were recorded in the Tavush Region (north-eastern part) and Armavir Region (south-western part) of Armenia in 2020–2022.
本文介绍了亚美尼亚Argiope属的第一个记录,Argiope是Araneidae家族的圆织蜘蛛。Argiope bruennichi (Scopoli, 1772)雌性蜘蛛2只在北部Lori地区发现,Argiope lobata (Pallas, 1772)雌性蜘蛛2只于2020-2022年在亚美尼亚东北部Tavush地区和西南部Armavir地区发现。
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引用次数: 0
PHENOLIC COMPOUNDS IN SOME WIDELY DISTRIBUTED MEDICINAL MUSHROOM SPECIES IN ARMENIA 亚美尼亚一些广泛分布的药用蘑菇种类中的酚类化合物
Pub Date : 2022-05-03 DOI: 10.46991/pysu:b/2022.56.1.068
Vahagn S. Gevorgyan
Polyphenols constitute one of the most numerous and ubiquitous groups of fungal metabolites and are an integral part of the human diet. These molecules can be met as simple phenolic compounds or highly polymerized compounds. Recent interest in phenolic compounds has increased, owing to their antioxidant capacity. Besides, these compounds can have possible beneficial implications in human health, such as in the treatment and prevention of cancer, cardiovascular diseases, and other pathologies. Since there is little research done in Armenia, the data is scattered. This review aims to combine domestic and abroad papers on this matter.
多酚是真菌代谢产物中数量最多、最普遍的一类,是人类饮食中不可或缺的一部分。这些分子可以是简单的酚类化合物或高度聚合的化合物。由于酚类化合物具有抗氧化能力,近年来人们对它们的兴趣有所增加。此外,这些化合物可能对人类健康有益,例如治疗和预防癌症、心血管疾病和其他病理。由于在亚美尼亚进行的研究很少,数据很分散。本文旨在结合国内外关于这一问题的论文进行综述。
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引用次数: 0
STUDY OF ASPIRIN EFFECT ON STABILITY OF MEMBRANES OF RAT BLOOD ERYTHROCYTES BY ACIDIC HEMOLYSIS METHOD 酸性溶血法研究阿司匹林对大鼠红细胞膜稳定性的影响
Pub Date : 2022-05-03 DOI: 10.46991/pysu:b/2022.56.1.056
M. Mikaelyan, M. Shahinyan, G. Poghosyan, Evelina G. Sargsyan, Anahit V. Nerkararyan
In this work the effect of aspirin on stability of rat blood erythrocyte membranes was studied, using acidic hemolysis method. Hemolysis rate of erythrocytes was shown to depend on preservation time of rat erythrocyte suspension. With increasing of preservation time the original value of optic density of rat erythrocyte suspension decreases and hemolysis time reduces. It was also shown that in the presence of aspirin the membrane stability of low-stable erythrocytes increases, moreover, hemolysis duration enhances, while aspirin leads to decreasing of membrane stability of both high-stable and enhanced-stable erythrocytes of rats.
本文采用酸性溶血法研究阿司匹林对大鼠红细胞膜稳定性的影响。红细胞溶血率与红细胞悬液保存时间有关。随着保存时间的延长,大鼠红细胞悬液光密度原始值降低,溶血时间缩短。结果表明,阿司匹林可提高大鼠低稳定型红细胞的膜稳定性,延长溶血时间,而阿司匹林可降低大鼠高稳定型和增强稳定型红细胞的膜稳定性。
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引用次数: 1
期刊
Proceedings of the YSU B: Chemical and Biological Sciences
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