Pub Date : 2023-02-02DOI: 10.46991/pysu:b/2023.57.1.001
K. Grigoryan, H. Shilajyan, Roza G. Harutyunyan
Molecular interactions of L-tyrosine in dimethyl sulfoxide (DMSO) aqueous solutions have been studied using electronic absorption and fluorescence spectro¬scopy methods. It has been shown that at low concentrations of DMSO in aqueous media hetero-associates such as 1DMSO:1H2O or 1DMSO:2H2O are formed, while at high concentrations, associates of a more complex structure (mixed clusters) are formed that interact with L-tyrosine to form complex compounds.
{"title":"MOLECULAR INTERACTIONS OF L-TYROSINE IN DIMETHYL SULFOXIDE AQUEOUS SOLUTIONS. PART 1: ELECTRONIC ABSORPTION AND FLUORESCENCE SPECTROSCOPY STUDIES","authors":"K. Grigoryan, H. Shilajyan, Roza G. Harutyunyan","doi":"10.46991/pysu:b/2023.57.1.001","DOIUrl":"https://doi.org/10.46991/pysu:b/2023.57.1.001","url":null,"abstract":"Molecular interactions of L-tyrosine in dimethyl sulfoxide (DMSO) aqueous solutions have been studied using electronic absorption and fluorescence spectro¬scopy methods. It has been shown that at low concentrations of DMSO in aqueous media hetero-associates such as 1DMSO:1H2O or 1DMSO:2H2O are formed, while at high concentrations, associates of a more complex structure (mixed clusters) are formed that interact with L-tyrosine to form complex compounds.","PeriodicalId":20692,"journal":{"name":"Proceedings of the YSU B: Chemical and Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79495070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-28DOI: 10.46991/pysu:b/2022.56.3.253
Ruzanna E. Matevosyan
In the current research, we investigated soil micromycetes-decomposers around Kajaran City. As a result of the studies, 30 micromycetes were identified as belonging to 16 genera of which 1 belongs to Basidiomycota, 1 – to Mortierellomycota, 3 – to Mucoromycota, and 11 – to Ascomycota. Changes in the species composition and some morphological features of soil fungi are observed depending on the industrial pollution.
{"title":"MICROMYCETES OF CONTAMINATED SOILS AROUND KAJARAN CITY (ARMENIA)","authors":"Ruzanna E. Matevosyan","doi":"10.46991/pysu:b/2022.56.3.253","DOIUrl":"https://doi.org/10.46991/pysu:b/2022.56.3.253","url":null,"abstract":"In the current research, we investigated soil micromycetes-decomposers around Kajaran City. As a result of the studies, 30 micromycetes were identified as belonging to 16 genera of which 1 belongs to Basidiomycota, 1 – to Mortierellomycota, 3 – to Mucoromycota, and 11 – to Ascomycota. Changes in the species composition and some morphological features of soil fungi are observed depending on the industrial pollution.","PeriodicalId":20692,"journal":{"name":"Proceedings of the YSU B: Chemical and Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72578321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-28DOI: 10.46991/pysu:b/2022.56.3.259
Narine A. Zakaryan, Vahagn S. Gevorgyan, Nune Kartashyan, S. Nanagulyan
Holoparasitic plants of the family Orobanchaceae are represented by 36 species belonging to 4 genera in Armenia. Despite the fact that the secondary metabolites and their pharmacological properties are not well studied, the limited data on this topic is already a promising source for different bioactive compounds. In this paper, we are representing the antioxidant properties and phenolic content of four species belonging to Orobanchaceae family. The highest antioxidant activities were recorded for Orobanche kurdica alcoholic extract. Water soluble phenols may explain the great antioxidant capacity of the aqueous extract of Diphelypaea tournefortii.
{"title":"ASSESSMENT OF ANTIOXIDANT ACTIVITY OF SOME OROBANCHACEAE SPECIES FROM ARMENIA","authors":"Narine A. Zakaryan, Vahagn S. Gevorgyan, Nune Kartashyan, S. Nanagulyan","doi":"10.46991/pysu:b/2022.56.3.259","DOIUrl":"https://doi.org/10.46991/pysu:b/2022.56.3.259","url":null,"abstract":"Holoparasitic plants of the family Orobanchaceae are represented by 36 species belonging to 4 genera in Armenia. Despite the fact that the secondary metabolites and their pharmacological properties are not well studied, the limited data on this topic is already a promising source for different bioactive compounds. In this paper, we are representing the antioxidant properties and phenolic content of four species belonging to Orobanchaceae family. The highest antioxidant activities were recorded for Orobanche kurdica alcoholic extract. Water soluble phenols may explain the great antioxidant capacity of the aqueous extract of Diphelypaea tournefortii.","PeriodicalId":20692,"journal":{"name":"Proceedings of the YSU B: Chemical and Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80966420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-28DOI: 10.46991/pysu:b/2022.56.3.231
Zvart H. Movsisyan
The study of the interaction of phenothiazine dye methylene blue (MB) and phenantridine dye ethidium bromide (EtBr) with synthetic single-stranded (ss)-poly(rA) has been carried out at the ionic strength of the solution 0.1 mol, in wide interval of ligand/phosphate ratio change – P/D, by the method of absorption spectroscopy. The binding curves of MB and EtBr with ss-poly(rA) were obtained. MB was shown to interact with this polynucleotide cooperatively, while for EtBr the cooperativity was not revealed. The binding parameters of MB and EtBr with ss-poly(rA) were determined – association constant K and base number n per binding site. It was shown that both ligands bind to ss-poly(rA) at least by two modes, though the affinity of MB to the polynucleotide is higher by an order, than that of EtBr.
{"title":"STUDY OF THE INTERACTION OF SINGLE-STRANDED POLYRIBOADENYLIC ACID WITH ETHIDIUM BROMIDE AND METHYLENE BLUE","authors":"Zvart H. Movsisyan","doi":"10.46991/pysu:b/2022.56.3.231","DOIUrl":"https://doi.org/10.46991/pysu:b/2022.56.3.231","url":null,"abstract":"The study of the interaction of phenothiazine dye methylene blue (MB) and phenantridine dye ethidium bromide (EtBr) with synthetic single-stranded (ss)-poly(rA) has been carried out at the ionic strength of the solution 0.1 mol, in wide interval of ligand/phosphate ratio change – P/D, by the method of absorption spectroscopy. The binding curves of MB and EtBr with ss-poly(rA) were obtained. MB was shown to interact with this polynucleotide cooperatively, while for EtBr the cooperativity was not revealed. The binding parameters of MB and EtBr with ss-poly(rA) were determined – association constant K and base number n per binding site. It was shown that both ligands bind to ss-poly(rA) at least by two modes, though the affinity of MB to the polynucleotide is higher by an order, than that of EtBr.","PeriodicalId":20692,"journal":{"name":"Proceedings of the YSU B: Chemical and Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80913424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-28DOI: 10.46991/pysu:b/2022.56.3.225
L. Minasbekyan, M. Parsadanyan
The effect of various doses of γ-irradiation on the biochemical composition of the nuclear fractions of wheat seedlings has been investigated. The research results indicate a direct dependence of changes in the biochemical composition of nuclear membranes and soluble nuclear fraction on the magnitude of the radiation dose. A change in the protein content in the fraction of the nuclear membrane and in the soluble nuclear fraction of the cell nuclei of the seedlings of irradiated seeds was observed. The data obtained indicate that ionizing radiation as a result of chain reactions changes the content of nuclear fractions, which in turn leads to a change and disruption of the functional ability of the nucleus.
{"title":"WHEAT PROTEIN INCREASING THROUGH RADIATION TECHNOLOGIES","authors":"L. Minasbekyan, M. Parsadanyan","doi":"10.46991/pysu:b/2022.56.3.225","DOIUrl":"https://doi.org/10.46991/pysu:b/2022.56.3.225","url":null,"abstract":"The effect of various doses of γ-irradiation on the biochemical composition of the nuclear fractions of wheat seedlings has been investigated. The research results indicate a direct dependence of changes in the biochemical composition of nuclear membranes and soluble nuclear fraction on the magnitude of the radiation dose. A change in the protein content in the fraction of the nuclear membrane and in the soluble nuclear fraction of the cell nuclei of the seedlings of irradiated seeds was observed. The data obtained indicate that ionizing radiation as a result of chain reactions changes the content of nuclear fractions, which in turn leads to a change and disruption of the functional ability of the nucleus.","PeriodicalId":20692,"journal":{"name":"Proceedings of the YSU B: Chemical and Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82626060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-28DOI: 10.46991/pysu:b/2022.56.3.218
M. Mikaelyan, M. Shahinyan, S. Grigoryan, M. Parsadanyan
In the present work the effect of resonant electromagnetic irradiation (EMI) and acetylsalicylic acid (ASA) on value change of the surface tension coefficient of human blood plasma has been studied. It was shown that both the plasma irradiation and the ASA insertion into plasma result in changing of the surface tension coefficient of human blood plasma, as well as affect the surface buffering. These changes are due to the fact that the mentioned factors invoke conformational alterations in the structures of plasma proteins, though we assume that ASA contributes in stabilization of structural changes of proteins.
{"title":"EFFECT OF RESONANT ELECTROMAGNETIC IRRADIATION AND ASPIRIN ON SURFACE TENSION OF HUMAN BLOOD PLASMA $IN~VITRO$","authors":"M. Mikaelyan, M. Shahinyan, S. Grigoryan, M. Parsadanyan","doi":"10.46991/pysu:b/2022.56.3.218","DOIUrl":"https://doi.org/10.46991/pysu:b/2022.56.3.218","url":null,"abstract":"In the present work the effect of resonant electromagnetic irradiation (EMI) and acetylsalicylic acid (ASA) on value change of the surface tension coefficient of human blood plasma has been studied. It was shown that both the plasma irradiation and the ASA insertion into plasma result in changing of the surface tension coefficient of human blood plasma, as well as affect the surface buffering. These changes are due to the fact that the mentioned factors invoke conformational alterations in the structures of plasma proteins, though we assume that ASA contributes in stabilization of structural changes of proteins.","PeriodicalId":20692,"journal":{"name":"Proceedings of the YSU B: Chemical and Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76384760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-28DOI: 10.46991/pysu:b/2022.56.3.275
E. Gevorkyan, L. Ghukasyan, K. V. Baghdasaryan, Anahit H. Byglaryan
The change in the functional state of the body of first-year students under the influence of physical activity was studied. As the analysis of the obtained data showed, running at a distance of 500 m, both for girls and boys, leads to unidirectional shifts in all the studied indicators of varying severity. The functional systems of the organism of first-year students of the university react to physical activity by the tension of the sympathetic circuit of the regulation of adaptive mechanisms.
{"title":"PHYSIOLOGICAL INDICATORS OF STUDENTS AFTER RUNNING A DISTANCE OF 500 METERS","authors":"E. Gevorkyan, L. Ghukasyan, K. V. Baghdasaryan, Anahit H. Byglaryan","doi":"10.46991/pysu:b/2022.56.3.275","DOIUrl":"https://doi.org/10.46991/pysu:b/2022.56.3.275","url":null,"abstract":"The change in the functional state of the body of first-year students under the influence of physical activity was studied. As the analysis of the obtained data showed, running at a distance of 500 m, both for girls and boys, leads to unidirectional shifts in all the studied indicators of varying severity. The functional systems of the organism of first-year students of the university react to physical activity by the tension of the sympathetic circuit of the regulation of adaptive mechanisms.","PeriodicalId":20692,"journal":{"name":"Proceedings of the YSU B: Chemical and Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80743555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-28DOI: 10.46991/pysu:b/2022.56.3.239
Irina A. Badalyan, A. Poladyan
The processes of lipid peroxidation (LPO) were studied in rat brain and liver tissues under normal conditions, as well as under conditions of hypobaric hypoxia, since it is known that hypoxia causes the development of LPO in the cells of organism tissues. It has been also studied the influense of dry flowers of Trifolium pratense L. (red clover) and Crataegus laevigata (midland hawthorn) on lipid peroxidation, which triggers by hypobaric hypoxia. It has been shown, that the end product of lipid peroxidation malondialdehyde in rat brain and liver tissues was significantly decreased with the addition of plants flowers to the diet of animals, especially in the case of C. laevigata.
{"title":"THE EFFECT OF ANTIOXIDANT ACTIVITY OF PLANTS $TRIFOLIUM~PRATENSE$ L. AND $CRATAEGUS~LAEVIGATA$ ON LIPID PEROXIDATION IN RAT'S TISSUES EXPOSED TO HYPOBARIC HYPOXIA","authors":"Irina A. Badalyan, A. Poladyan","doi":"10.46991/pysu:b/2022.56.3.239","DOIUrl":"https://doi.org/10.46991/pysu:b/2022.56.3.239","url":null,"abstract":"The processes of lipid peroxidation (LPO) were studied in rat brain and liver tissues under normal conditions, as well as under conditions of hypobaric hypoxia, since it is known that hypoxia causes the development of LPO in the cells of organism tissues. It has been also studied the influense of dry flowers of Trifolium pratense L. (red clover) and Crataegus laevigata (midland hawthorn) on lipid peroxidation, which triggers by hypobaric hypoxia. It has been shown, that the end product of lipid peroxidation malondialdehyde in rat brain and liver tissues was significantly decreased with the addition of plants flowers to the diet of animals, especially in the case of C. laevigata.","PeriodicalId":20692,"journal":{"name":"Proceedings of the YSU B: Chemical and Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89413754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-28DOI: 10.46991/pysu:b/2022.56.3.266
R. A. Shushanyan, Anna V. Grigoryan, A. Karapetyan
Hypobaric hypoxia causes degenerative changes in different parts of the brain, which leads to the formation of cerebral edema. This study aimed to investigate the morphohistological changes in the brain of rats exposed to hypobaric hypoxia. In particular, the hippocampus, cerebral cortex, and striatum were studied. Animals were exposed to hypobaric hypoxia for 10 days. Histomorphological examination showed degenerative changes in the hippocampus, dentate gyrus, and striatum, accompanied by pyknosis of neuronal nuclei, cytoplasmic vacuolization, and neuronal shrinkage, while the cortex of the large hemispheres remained almost intact. Histological studies showed that different parts of the brain respond to hypoxic conditions with different manifestations.
{"title":"HYPOBARIC HYPOXIA CAUSES HISTOMORPHOLOGICAL ALTERATIONS IN THE DIFFERENT PARTS OF THE RAT'S BRAIN","authors":"R. A. Shushanyan, Anna V. Grigoryan, A. Karapetyan","doi":"10.46991/pysu:b/2022.56.3.266","DOIUrl":"https://doi.org/10.46991/pysu:b/2022.56.3.266","url":null,"abstract":"Hypobaric hypoxia causes degenerative changes in different parts of the brain, which leads to the formation of cerebral edema. This study aimed to investigate the morphohistological changes in the brain of rats exposed to hypobaric hypoxia. In particular, the hippocampus, cerebral cortex, and striatum were studied. Animals were exposed to hypobaric hypoxia for 10 days. Histomorphological examination showed degenerative changes in the hippocampus, dentate gyrus, and striatum, accompanied by pyknosis of neuronal nuclei, cytoplasmic vacuolization, and neuronal shrinkage, while the cortex of the large hemispheres remained almost intact. Histological studies showed that different parts of the brain respond to hypoxic conditions with different manifestations.","PeriodicalId":20692,"journal":{"name":"Proceedings of the YSU B: Chemical and Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75398412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-28DOI: 10.46991/pysu:b/2022.56.3.245
Narine K. Hayrapetyan
Modeling of methemoglobinemia (MetHb) led to a violation of antioxidant protection, which was manifested by a decrease in the activity of enzymes of the primary link of the antioxidant system – superoxide dismutase and catalase in blood plasma and brain homogenates of experimental animals. The state of MetHb also led to an increase in the concentration of malondialdehyde, an indicator of lipid peroxidation, as well as an indicator of oxidative modification of proteins. It has been established that the antihypoxic activity in the application of succinic acid significantly suppresses the biochemical changes caused by the hypoxic state.
{"title":"STUDY OF THE ANTIHYPOXIC EFFICIENCY OF SUCCINATE UNDER THE CONDITIONS OF METHEMOGLOBINEMIA","authors":"Narine K. Hayrapetyan","doi":"10.46991/pysu:b/2022.56.3.245","DOIUrl":"https://doi.org/10.46991/pysu:b/2022.56.3.245","url":null,"abstract":"Modeling of methemoglobinemia (MetHb) led to a violation of antioxidant protection, which was manifested by a decrease in the activity of enzymes of the primary link of the antioxidant system – superoxide dismutase and catalase in blood plasma and brain homogenates of experimental animals. The state of MetHb also led to an increase in the concentration of malondialdehyde, an indicator of lipid peroxidation, as well as an indicator of oxidative modification of proteins. It has been established that the antihypoxic activity in the application of succinic acid significantly suppresses the biochemical changes caused by the hypoxic state.","PeriodicalId":20692,"journal":{"name":"Proceedings of the YSU B: Chemical and Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82037329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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