Pub Date : 2023-07-07DOI: 10.46991/pysu:b/2023.57.2.133
Agapi G. Hovhannisyan, N. R. Hakobyan, Zh. V. Yavroyan, E. Gevorgyan
The antitumor agent cisplatin and progesterone separate and joint action on content of total phospholipids and their individual fractions in nuclei from rat thymus cells were investigated. When used separately, cisplatin and progesterone exhibit its own distinctive properties. Cisplatin reduces, and progesterone, on the contrary, increases the content of total phospholipids. When used together, the effects of these drugs are summed up. Thus total phospholipid content of rat thymus cell nuclei increases by 17% after the joint injection of cisplatin and progesterone. These changes have different effects on quantities of individual phospholipid fractions. It is assumed that the effects of cisplatin and progesterone, both in their separate and joint action, can be mediated by quantitative changes in internuclear lipids that in turn can regulate the main functions of the cell nuclei.
{"title":"CISPLATIN AND PROGESTERONE SEPARATE AND JOINT ACTION ON RAT THYMUS NUCLEAR PHOSPHOLIPIDS COMPOSITION AND CONTENT","authors":"Agapi G. Hovhannisyan, N. R. Hakobyan, Zh. V. Yavroyan, E. Gevorgyan","doi":"10.46991/pysu:b/2023.57.2.133","DOIUrl":"https://doi.org/10.46991/pysu:b/2023.57.2.133","url":null,"abstract":"The antitumor agent cisplatin and progesterone separate and joint action on content of total phospholipids and their individual fractions in nuclei from rat thymus cells were investigated. When used separately, cisplatin and progesterone exhibit its own distinctive properties. Cisplatin reduces, and progesterone, on the contrary, increases the content of total phospholipids. When used together, the effects of these drugs are summed up. Thus total phospholipid content of rat thymus cell nuclei increases by 17% after the joint injection of cisplatin and progesterone. These changes have different effects on quantities of individual phospholipid fractions. It is assumed that the effects of cisplatin and progesterone, both in their separate and joint action, can be mediated by quantitative changes in internuclear lipids that in turn can regulate the main functions of the cell nuclei.","PeriodicalId":20692,"journal":{"name":"Proceedings of the YSU B: Chemical and Biological Sciences","volume":"11 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139361894","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-07DOI: 10.46991/pysu:b/2023.57.2.141
Anahit H. Shirvanyan
Bioethanol production is in high demand due to its potential to replace transportation fuel and its ability to be renewable and long-lasting. Therefore, the bioconversion of fermentable sugars to ethanol is of central importance. The work aimed to explore yeast properties changes during fermentation at different pHs and temperature conditions. Biomass production, specific growth rate (SGR), fermentation products, and metabolite composition and quantity changes were studied at 25℃, 30℃, and 37℃ as well as pH 5 and pH 6.5 conditions. SGR data shows that at 25℃ and 30℃ pH 5 is optimal for yeast biomass production. At pH 5.0–6.5, the biomass production of the ATCC 9804 strain almost 2 times exceeds the same parameter for another strain. The highest biomass production was detected at 30℃ and pH 5. Ethanol production by yeast increases as the growth temperature decreases at pH 5 in contrast to pH 6.5, where the temperature changes within 30–37℃ range have no significant effect on it in both strains. At 30℃ ethanol concentration in the extracellular medium reached ~117 mM in the case of ATCC 13007 strain, whereas for ATCC 9804 the same parameter was 1.4-fold lower. Both strains had the same substrate assimilation rate. Glycerol production reduces with increasing growth temperature and pH; the highest glycerol concentration (6.1 mM) was observed within 32 h growth of ATCC 9804 strain at 25℃, pH 5 and reaches 5.5 mM within 24 h growth of ATCC 13007 strain under the same conditions. At pH 5 and 6.5, the free energy for glycerol production was 2.3-fold and 4-fold higher compared to that of ethanol and acetate production processes in both strains. Gibbs free energy of ethanol production reaches the lowest value compared to the same parameter of acetate and glycerol production at pH 5, suggesting a preference for the alcoholic fermentation metabolic pathway under these conditions. The lowest acetate production was observed after 24 hours of growth of the ATCC 13007 strain at 37°C and pH 5, with ΔG = 173.9 kJ/mol. Obtained data highlights temperature stress mechanisms regulation of yeast and can be used for improving ethanol production processes and yeast genetic modification tools.
{"title":"EVALUATION OF ETHANOL AND BIOMASS PRODUCTION RATE BY DIFFERENT $SACCHAROMYCES~CEREVISIAE$ STRAINS DEPENDING ON EXTERNAL pH AND TEMPERATURE","authors":"Anahit H. Shirvanyan","doi":"10.46991/pysu:b/2023.57.2.141","DOIUrl":"https://doi.org/10.46991/pysu:b/2023.57.2.141","url":null,"abstract":"Bioethanol production is in high demand due to its potential to replace transportation fuel and its ability to be renewable and long-lasting. Therefore, the bioconversion of fermentable sugars to ethanol is of central importance. The work aimed to explore yeast properties changes during fermentation at different pHs and temperature conditions. Biomass production, specific growth rate (SGR), fermentation products, and metabolite composition and quantity changes were studied at 25℃, 30℃, and 37℃ as well as pH 5 and pH 6.5 conditions. SGR data shows that at 25℃ and 30℃ pH 5 is optimal for yeast biomass production. At pH 5.0–6.5, the biomass production of the ATCC 9804 strain almost 2 times exceeds the same parameter for another strain. The highest biomass production was detected at 30℃ and pH 5. Ethanol production by yeast increases as the growth temperature decreases at pH 5 in contrast to pH 6.5, where the temperature changes within 30–37℃ range have no significant effect on it in both strains. At 30℃ ethanol concentration in the extracellular medium reached ~117 mM in the case of ATCC 13007 strain, whereas for ATCC 9804 the same parameter was 1.4-fold lower. Both strains had the same substrate assimilation rate. Glycerol production reduces with increasing growth temperature and pH; the highest glycerol concentration (6.1 mM) was observed within 32 h growth of ATCC 9804 strain at 25℃, pH 5 and reaches 5.5 mM within 24 h growth of ATCC 13007 strain under the same conditions. At pH 5 and 6.5, the free energy for glycerol production was 2.3-fold and 4-fold higher compared to that of ethanol and acetate production processes in both strains. Gibbs free energy of ethanol production reaches the lowest value compared to the same parameter of acetate and glycerol production at pH 5, suggesting a preference for the alcoholic fermentation metabolic pathway under these conditions. The lowest acetate production was observed after 24 hours of growth of the ATCC 13007 strain at 37°C and pH 5, with ΔG = 173.9 kJ/mol. Obtained data highlights temperature stress mechanisms regulation of yeast and can be used for improving ethanol production processes and yeast genetic modification tools.","PeriodicalId":20692,"journal":{"name":"Proceedings of the YSU B: Chemical and Biological Sciences","volume":"45 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139361619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-07DOI: 10.46991/pysu:b/2023.57.2.126
Zvart H. Movsisyan
The study of the interaction of intercalators ethidium bromide (EtBr), methylene blue (MB) and groove binding compound Hoechst 33258 (H33258) with single-stranded synthetic polyribonucleotides poly(rA) and poly(rU) has been carried out by the method of UV-melting, at ionic strengths of the solution 0.04 M and 0.1 M. Stirring of poly(rA) and poly(rU) by equal-molar concentrations was shown to result in hybridization with formation of double-stranded (ds-) structure poly(rA)-poly(rU). It was revealed that EtBr, with higher degree in comparison with MB, stimulates hybridization and stabilizes the formed ds-structure poly(rA)-poly(rU). It was also found out that the hybridization process and affinity of EtBr and MB depend on the ionic strength of the solution and these processes occur much more effectively at the ionic strength 0.04 M. On the other hand, it was shown that the groove binding ligand H33258 practically does not affect the stabilization of the formed ds-structure poly(rA)-poly(rU).
通过紫外熔融法研究了插入剂溴化乙锭（EtBr）、亚甲基蓝（MB）和沟槽结合化合物 Hoechst 33258（H33258）与单链合成多核苷酸聚（rA）和聚（rU）的相互作用，溶液离子强度分别为 0.04 M 和 0.1 M。等摩尔浓度的聚(rA)和聚(rU)在搅拌后会发生杂交，形成双链（ds-）结构的聚(rA)-聚(rU)。结果表明，与 MB 相比，EtBr 的浓度更高，它能刺激杂交并稳定所形成的 ds 结构聚(rA)-聚(rU)。研究还发现，EtBr 和 MB 的杂化过程和亲和力取决于溶液的离子强度，在离子强度为 0.04 M 时，杂化过程会更有效。
{"title":"STUDY OF HYBRIDIZATION OF COMPLEMENTARY SINGLE-STRANDED POLYNUCLEOTIDES POLY(rA) AND POLY(rU)","authors":"Zvart H. Movsisyan","doi":"10.46991/pysu:b/2023.57.2.126","DOIUrl":"https://doi.org/10.46991/pysu:b/2023.57.2.126","url":null,"abstract":"The study of the interaction of intercalators ethidium bromide (EtBr), methylene blue (MB) and groove binding compound Hoechst 33258 (H33258) with single-stranded synthetic polyribonucleotides poly(rA) and poly(rU) has been carried out by the method of UV-melting, at ionic strengths of the solution 0.04 M and 0.1 M. Stirring of poly(rA) and poly(rU) by equal-molar concentrations was shown to result in hybridization with formation of double-stranded (ds-) structure poly(rA)-poly(rU). It was revealed that EtBr, with higher degree in comparison with MB, stimulates hybridization and stabilizes the formed ds-structure poly(rA)-poly(rU). It was also found out that the hybridization process and affinity of EtBr and MB depend on the ionic strength of the solution and these processes occur much more effectively at the ionic strength 0.04 M. On the other hand, it was shown that the groove binding ligand H33258 practically does not affect the stabilization of the formed ds-structure poly(rA)-poly(rU).","PeriodicalId":20692,"journal":{"name":"Proceedings of the YSU B: Chemical and Biological Sciences","volume":"50 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139361782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-06DOI: 10.46991/pysu:b/2023.57.2.090
S. Hayrapetyan, M. S. Hayrapetyan, H. H. Khachatryan
Inductively coupled plasma mass spectrometry (ISP-MS) and scanning electron microscope-X-ray fluorescence (SEM-XRF) methods were used for the investigation of olivine sorption properties. It has been shown, that olivine may be applied as a cheap and available material for the sorption of metals, including heavy metals, from wastewater. The comparatively low sorption capacity of olivine may pay off by its accessibility and cheapness. The mechanism of adsorption on the olivine surface may be described as the substitution of magnesium ions by cations of various metals. One trend can be fixed – when ionic radius of the metal cations increases, their sorption increases as well. However, it is hardly possible to tie everything with the ionic radius of metal ion. The specificity of each element is also important.
{"title":"OLIVINE-BASED SORBENTS FOR REMOVAL OF HEAVY METALS","authors":"S. Hayrapetyan, M. S. Hayrapetyan, H. H. Khachatryan","doi":"10.46991/pysu:b/2023.57.2.090","DOIUrl":"https://doi.org/10.46991/pysu:b/2023.57.2.090","url":null,"abstract":"Inductively coupled plasma mass spectrometry (ISP-MS) and scanning electron microscope-X-ray fluorescence (SEM-XRF) methods were used for the investigation of olivine sorption properties. It has been shown, that olivine may be applied as a cheap and available material for the sorption of metals, including heavy metals, from wastewater. The comparatively low sorption capacity of olivine may pay off by its accessibility and cheapness. The mechanism of adsorption on the olivine surface may be described as the substitution of magnesium ions by cations of various metals. One trend can be fixed – when ionic radius of the metal cations increases, their sorption increases as well. However, it is hardly possible to tie everything with the ionic radius of metal ion. The specificity of each element is also important.","PeriodicalId":20692,"journal":{"name":"Proceedings of the YSU B: Chemical and Biological Sciences","volume":"73 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139362211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-27DOI: 10.46991/pysu:b/2023.57.1.063
Marietta A. Karapetyan, Nonna Yu. Adamyan, Siranush R. Aghajanyan
The normal course of biochemical and physiological processes that ensure the vital activity of the organism, among other systems, also depends on the normal functioning of the respiratory system. A number of external and internal factors can affect the indicators of respiratory movements: frequency, depth, respiratory volume: atmospheric pressure, the amount of oxygen in the atmosphere, the ecological state of the environment, various pathologies of the organism, etc., which become the cause of diseases. In the risk group of these diseases are the inhabitants of the areas near the ecologically unfavorable environment, especially the open mines, particularly the school children of that region. It is possible to reduce these risks and prevent diseases of the mentioned systems by the method of special breathing exercises. Residents of ecological regions, in particular adolescents, are at risk. This risk can be reduced by using the breathing exercise method. The purpose of this study was testing the effectiveness of these exercises on the indicators of the respiratory system of teenagers in the Vardenis Region.
{"title":"CORRECTIVE EFFECT OF BREATHING EXERCISES ON THE HEALTH OF ECOLOGICALLY UNFAVORABLE REGIONAL SCHOOLS CHILDREN","authors":"Marietta A. Karapetyan, Nonna Yu. Adamyan, Siranush R. Aghajanyan","doi":"10.46991/pysu:b/2023.57.1.063","DOIUrl":"https://doi.org/10.46991/pysu:b/2023.57.1.063","url":null,"abstract":"The normal course of biochemical and physiological processes that ensure the vital activity of the organism, among other systems, also depends on the normal functioning of the respiratory system. A number of external and internal factors can affect the indicators of respiratory movements: frequency, depth, respiratory volume: atmospheric pressure, the amount of oxygen in the atmosphere, the ecological state of the environment, various pathologies of the organism, etc., which become the cause of diseases. In the risk group of these diseases are the inhabitants of the areas near the ecologically unfavorable environment, especially the open mines, particularly the school children of that region. It is possible to reduce these risks and prevent diseases of the mentioned systems by the method of special breathing exercises. Residents of ecological regions, in particular adolescents, are at risk. This risk can be reduced by using the breathing exercise method.\u0000The purpose of this study was testing the effectiveness of these exercises on the indicators of the respiratory system of teenagers in the Vardenis Region.","PeriodicalId":20692,"journal":{"name":"Proceedings of the YSU B: Chemical and Biological Sciences","volume":"10 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89650026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-27DOI: 10.46991/pysu:b/2023.57.1.041
N. Karapetyan, G. Ananyan, S. Haroutiunian
The silver-containing porphyrin Ag-meso-tetra(4-N-hydroxyethylpyridyl)porphyrin (AgTOEPyP4) was investigated as a potential anticancer drug using spectrophotometric, calorimetric and electrophoretic measurements. The studies were conducted on healthy, cancer-induced, and treated by AgTOEPyP4 white mice. DNA was isolated from the liver and tumor tissues of mice. Melting curves were studied in the presence of various stoichiometric concentrations of Mn2+ ions. The character of the DNA–Mn2+ interaction in the tumor differs from that in the norm due to defects in the secondary structure of tumor DNA, which is expressed in the DNA melting characteristics of DNA. It was revealed that the melting parameters, enthalpy, and electrophoretic mobility of DNA isolated from the tumor-bearing mice tissues differed from healthy DNA. It was shown that all studied parameters of the DNA isolated from the liver and tumor treated by AgTOEPyP4 mice approached the norm. The obtained results revealed that AgTOEPyP4 porphyrin has a pronounced therapeutic effect against sarcoma S180 and requires further investigation as an anticancer drug.
采用分光光度法、量热法和电泳法研究了含银卟啉ag -中四(4- n -羟乙基吡啶基)卟啉(AgTOEPyP4)作为一种潜在的抗癌药物。这些研究是在健康、癌症诱导和AgTOEPyP4治疗的小白鼠身上进行的。从小鼠的肝脏和肿瘤组织中分离DNA。研究了不同化学计量浓度的Mn2+离子存在下的熔化曲线。肿瘤中DNA - mn2 +相互作用的特点不同于正常情况，这是由于肿瘤DNA的二级结构存在缺陷，表现为DNA的DNA熔化特性。结果表明，从荷瘤小鼠组织中分离的DNA的熔化参数、焓和电泳迁移率与健康DNA不同。结果表明，经AgTOEPyP4处理的小鼠肝脏和肿瘤中分离的DNA的所有研究参数均接近正常值。结果表明，AgTOEPyP4卟啉对S180肉瘤具有明显的治疗作用，作为一种抗癌药物有待进一步研究。
{"title":"ANTICANCER EFFECT OF AgTOEPyP4 PORPHYRIN","authors":"N. Karapetyan, G. Ananyan, S. Haroutiunian","doi":"10.46991/pysu:b/2023.57.1.041","DOIUrl":"https://doi.org/10.46991/pysu:b/2023.57.1.041","url":null,"abstract":"The silver-containing porphyrin Ag-meso-tetra(4-N-hydroxyethylpyridyl)porphyrin (AgTOEPyP4) was investigated as a potential anticancer drug using spectrophotometric, calorimetric and electrophoretic measurements. The studies were conducted on healthy, cancer-induced, and treated by AgTOEPyP4 white mice. DNA was isolated from the liver and tumor tissues of mice. Melting curves were studied in the presence of various stoichiometric concentrations of Mn2+ ions. The character of the DNA–Mn2+ interaction in the tumor differs from that in the norm due to defects in the secondary structure of tumor DNA, which is expressed in the DNA melting characteristics of DNA. It was revealed that the melting parameters, enthalpy, and electrophoretic mobility of DNA isolated from the tumor-bearing mice tissues differed from healthy DNA. It was shown that all studied parameters of the DNA isolated from the liver and tumor treated by AgTOEPyP4 mice approached the norm. The obtained results revealed that AgTOEPyP4 porphyrin has a pronounced therapeutic effect against sarcoma S180 and requires further investigation as an anticancer drug.","PeriodicalId":20692,"journal":{"name":"Proceedings of the YSU B: Chemical and Biological Sciences","volume":"19 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78071141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-27DOI: 10.46991/pysu:b/2023.57.1.054
Anush M. Babayan, N. Sahakyan
Antimicrobial activity of Ribes nigrum L. and Ribes rubrum L. leaf extracts was determined by disk-diffusion method as well as specific growth rate and generation succeeding factor determination. Different Gram-positive (Bacillus subtilis WT-A, Staphylococcus aureus MDC 5233, Enterococcus hirae ATCC 9790) and Gram-negative (Escherichia coli ATCC 25922, Salmonella typhimurium MDC1754, ampicillin resistant E. coli dhpα-pUC18 and kanamycin resistant E. coli pARG25) bacteria and yeasts (Saccharomyces cerevisiae ATCC 9804 and S. cerevisiae ATCC 13007) were used as test-microorganisms. 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay was applied to measure the radical scavenging capacity of extracts obtained from Ribes spp. R. rubrum and R. nigrum extracts antiradical activity expressed with IC50 value of 91.2±1.69 µg‧mL–1 and 66.01±1.65 µg‧mL–1, respectively. The total flavonoid content in plant extracts was determined employing AlCl3 colorimetric assay, and the values were 34.71±0.63 and 49.99±0.86 μg QE‧mg–1 for R. rubrum and R. nigrum extracts, respectively. Total phenolic content of studied extracts was investigated by Folin–Ciocalteu assay. The contents for R. rubrum and R. nigrum extracts were 133.12±6.65 and 167.15±7.29 μg GAE‧mg–1, respectively. Thus, these plants can be considered as potential sources of biologically active substances.
{"title":"ANTIMICROBIAL ACTIVITY OF ETHANOL EXTRACTS OF $RIBES~NIGRUM$ AND $RIBES~RUBRUM$ LEAVES","authors":"Anush M. Babayan, N. Sahakyan","doi":"10.46991/pysu:b/2023.57.1.054","DOIUrl":"https://doi.org/10.46991/pysu:b/2023.57.1.054","url":null,"abstract":"Antimicrobial activity of Ribes nigrum L. and Ribes rubrum L. leaf extracts was determined by disk-diffusion method as well as specific growth rate and generation succeeding factor determination. Different Gram-positive (Bacillus subtilis WT-A, Staphylococcus aureus MDC 5233, Enterococcus hirae ATCC 9790) and Gram-negative (Escherichia coli ATCC 25922, Salmonella typhimurium MDC1754, ampicillin resistant E. coli dhpα-pUC18 and kanamycin resistant E. coli pARG25) bacteria and yeasts (Saccharomyces cerevisiae ATCC 9804 and S. cerevisiae ATCC 13007) were used as test-microorganisms. 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay was applied to measure the radical scavenging capacity of extracts obtained from Ribes spp. R. rubrum and R. nigrum extracts antiradical activity expressed with IC50 value of 91.2±1.69 µg‧mL–1 and 66.01±1.65 µg‧mL–1, respectively. The total flavonoid content in plant extracts was determined employing AlCl3 colorimetric assay, and the values were 34.71±0.63 and 49.99±0.86 μg QE‧mg–1 for R. rubrum and R. nigrum extracts, respectively. Total phenolic content of studied extracts was investigated by Folin–Ciocalteu assay. The contents for R. rubrum and R. nigrum extracts were 133.12±6.65 and 167.15±7.29 μg GAE‧mg–1, respectively. Thus, these plants can be considered as potential sources of biologically active substances.","PeriodicalId":20692,"journal":{"name":"Proceedings of the YSU B: Chemical and Biological Sciences","volume":"6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85649805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-27DOI: 10.46991/pysu:b/2023.57.1.032
T. Harutyunyan
Doxorubicin (DOX) is an antineoplastic drug with potent cardiotoxic activity due to selective accumulation and disruption of mitochondrial functioning in cardiomyocytes. Accumulating evidence indicates that DOX can also induce genotoxic side effects in other cells. The mutagenic effects of DOX in human peripheral blood leukocytes and THP-1 leukemia cells were analyzed using PCR. It was revealed that DOX could induce deletions of different sizes and loci of mitogenomes of normal human leukocytes and THP-1 leukemic cells.
{"title":"ANALYSIS OF DOXORUBICIN-INDUCED MITOCHONDRIAL DNA DELETIONS IN HUMAN BLOOD LEUKOCYTES AND THP-1 CELL LINE BY PCR","authors":"T. Harutyunyan","doi":"10.46991/pysu:b/2023.57.1.032","DOIUrl":"https://doi.org/10.46991/pysu:b/2023.57.1.032","url":null,"abstract":"Doxorubicin (DOX) is an antineoplastic drug with potent cardiotoxic activity due to selective accumulation and disruption of mitochondrial functioning in cardiomyocytes. Accumulating evidence indicates that DOX can also induce genotoxic side effects in other cells. The mutagenic effects of DOX in human peripheral blood leukocytes and THP-1 leukemia cells were analyzed using PCR. It was revealed that DOX could induce deletions of different sizes and loci of mitogenomes of normal human leukocytes and THP-1 leukemic cells.","PeriodicalId":20692,"journal":{"name":"Proceedings of the YSU B: Chemical and Biological Sciences","volume":"16 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78007979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-27DOI: 10.46991/pysu:b/2023.57.1.019
G. Poghosyan, M. Mikaelyan, P. Vardevanyan
In this work, the effect of low-intensity (non-thermal, flux capacity 0.06 mW/cm2) electromagnetic irradiation (EMI) of extremely high frequency (EHF) (42.2 GHz and 50.3 GHz) as pre-sowing treatment on germination, early growth and α-amylase activity of wheat seeds (Triticum aestivum L.) has been studied. The measurements conducted during seed germination were the germination energy and germination power, germination index, shoot and root length, as well as fresh weight. The laboratory experiment followed a completely randomized design, with four treatments (control, 5 min – T5, 10 min – T10, and 15 min – T15) and three replications. It was shown that EHF EMI pre-sowing treatment at both used frequencies favored the germination and growth of wheat seeds, and results were more expressed at a frequency of 50.3 GHz – the resonant frequency of water. We found that EMI with 50.3 GHz for 10 min significantly improved germination energy (by 6.5%) and germination power (by 7.6%). At the same time, shoot high (by 11%), root length (by 12%) and fresh weight (15%) were enhanced significantly at seedling stage compared with the control. Furthermore, the measured activity of α-amylase reviled that EMI-treatment increased the enzyme activity more than 2-fold, thereby probably promoting wheat germination.
本文研究了超低频(42.2 GHz和50.3 GHz)低强度(非热，通量0.06 mW/cm2)电磁辐射(EMI)播前处理对小麦种子萌发、早期生长和α-淀粉酶活性的影响。在种子萌发过程中测定萌发能和萌发力、萌发指数、茎长和根长以及鲜重。实验室试验采用完全随机设计，设4个处理(对照、5 min - T5、10 min - T10和15 min - T15)， 3个重复。结果表明，两种频率的EHF EMI播前处理均有利于小麦种子的萌发和生长，且在水的共振频率50.3 GHz处表现得更为明显。结果表明，50.3 GHz干扰10 min显著提高了种子萌发能(6.5%)和发芽力(7.6%)。苗期茎高(11%)、根长(12%)和鲜重(15%)均较对照显著提高。此外，α-淀粉酶活性的测定表明，emi处理使α-淀粉酶活性提高了2倍以上，从而可能促进了小麦的萌发。
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Pub Date : 2023-02-15DOI: 10.46991/pysu:b/2023.57.1.008
T. Ghochikyan, M. A. Samvelyan
On the basis of 3,5-disubstituted dihydrofuran-2(3H)-ones, hydrazides of substituted γ-hydroxybutyric acids were synthesized, which were further converted into the corresponding thiosemicarbazides and 1,2,4-triazoles. The synthesized compounds have not been previously described in the literature, and they cannot be obtained by any other way.
{"title":"SYNTHESIS OF NEW DERIVATIVES OF 1,2,4-TRIAZOLES","authors":"T. Ghochikyan, M. A. Samvelyan","doi":"10.46991/pysu:b/2023.57.1.008","DOIUrl":"https://doi.org/10.46991/pysu:b/2023.57.1.008","url":null,"abstract":"On the basis of 3,5-disubstituted dihydrofuran-2(3H)-ones, hydrazides of substituted γ-hydroxybutyric acids were synthesized, which were further converted into the corresponding thiosemicarbazides and 1,2,4-triazoles. The synthesized compounds have not been previously described in the literature, and they cannot be obtained by any other way.","PeriodicalId":20692,"journal":{"name":"Proceedings of the YSU B: Chemical and Biological Sciences","volume":"15 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89631976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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