Pub Date : 2021-12-23DOI: 10.46991/pysu:b/2021.55.3.232
J. Manoyan, L. Gabrielyan
Green algae are promising objects of biotechnology due to their high growth rates and the ability to produce biologically active compounds. Moreover, green algae are able of generating biohydrogen (H2), which is of interest in biofuels technology. In this study an application of distillers grains with solubles (DGS), the main waste generated during ethanol production, as a feedstock for enhancing the yield of H2 in green alga Chlorella vulgaris IBCE C-19 was investigated. The results obtained showed the possibility of using of this waste as a valuable source of natural biological compounds in H2 production.
{"title":"THE ETHANOL INDUSTRY WASTE AS A VALUABLE FEEDSTOCK FOR HYDROGEN PHOTOPRODUCTION BY GREEN ALGAE $CHLORELLA~VULGARIS$","authors":"J. Manoyan, L. Gabrielyan","doi":"10.46991/pysu:b/2021.55.3.232","DOIUrl":"https://doi.org/10.46991/pysu:b/2021.55.3.232","url":null,"abstract":"Green algae are promising objects of biotechnology due to their high growth rates and the ability to produce biologically active compounds. Moreover, green algae are able of generating biohydrogen (H2), which is of interest in biofuels technology. In this study an application of distillers grains with solubles (DGS), the main waste generated during ethanol production, as a feedstock for enhancing the yield of H2 in green alga Chlorella vulgaris IBCE C-19 was investigated. The results obtained showed the possibility of using of this waste as a valuable source of natural biological compounds in H2 production.","PeriodicalId":20692,"journal":{"name":"Proceedings of the YSU B: Chemical and Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74669326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-12-23DOI: 10.46991/pysu:b/2021.55.3.276
S. Mkrtchyan, R. Dunamalyan, M. Mardiyan, L. Ghukasyan, G. Mirijanyan
The aim of this research was to reveal an impact of anemia on the quality of life (QL) score, as well as on different subscales of children's QL. The subjects of the research were 3 months–3 years old 100 children selected from two pediatric polyclinics of Yerevan. QL were measured by QUALIN questionnaire. A significant decrease in the total score and other domains of the QUALIN questionnaire scores were indicative for an impact of anemia on QL of the early aged children. Most affected subscale in case of anemia was «Ability to remain alone».
{"title":"THE PROBLEM OF ANEMIA IN ARMENIA AMONG YOUNG CHILDREN","authors":"S. Mkrtchyan, R. Dunamalyan, M. Mardiyan, L. Ghukasyan, G. Mirijanyan","doi":"10.46991/pysu:b/2021.55.3.276","DOIUrl":"https://doi.org/10.46991/pysu:b/2021.55.3.276","url":null,"abstract":"The aim of this research was to reveal an impact of anemia on the quality of life (QL) score, as well as on different subscales of children's QL. The subjects of the research were 3 months–3 years old 100 children selected from two pediatric polyclinics of Yerevan. QL were measured by QUALIN questionnaire. A significant decrease in the total score and other domains of the QUALIN questionnaire scores were indicative for an impact of anemia on QL of the early aged children. Most affected subscale in case of anemia was «Ability to remain alone».","PeriodicalId":20692,"journal":{"name":"Proceedings of the YSU B: Chemical and Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84354235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-12-23DOI: 10.46991/pysu:b/2021.55.3.217
Anahit V. Nerkararyan, M. Mikaelyan, S. Grigoryan
In this work the effect of EMI EHF on change of the surface tension coefficient of human blood plasma under the effect of acetylsalicylic acid (ASA) as well as dependence of this parameter change on ASA effect duration and electromagnetic irradiation duration have been studied. It was shown that the plasma irradiation results in changing of the surface tension coefficient. At irradiation during 60 min the value of the given parameter increases, though, the further repeating irradiation does not induce an enhancement; some decrease is observed, but it remains higher, than the original value. It was also shown that ASA prevents development of relevant changes of blood plasma properties, particularly the value of the surface tension coefficient of plasma under the effect of EMI in millimeter range changes.
{"title":"EFFECT OF ELECTROMAGNETIC IRRADIATION AND ASPIRIN ON SURFACE TENSION OF HUMAN BLOOD PLASMA $IN~VITRO$","authors":"Anahit V. Nerkararyan, M. Mikaelyan, S. Grigoryan","doi":"10.46991/pysu:b/2021.55.3.217","DOIUrl":"https://doi.org/10.46991/pysu:b/2021.55.3.217","url":null,"abstract":"In this work the effect of EMI EHF on change of the surface tension coefficient of human blood plasma under the effect of acetylsalicylic acid (ASA) as well as dependence of this parameter change on ASA effect duration and electromagnetic irradiation duration have been studied. It was shown that the plasma irradiation results in changing of the surface tension coefficient. At irradiation during 60 min the value of the given parameter increases, though, the further repeating irradiation does not induce an enhancement; some decrease is observed, but it remains higher, than the original value. It was also shown that ASA prevents development of relevant changes of blood plasma properties, particularly the value of the surface tension coefficient of plasma under the effect of EMI in millimeter range changes.","PeriodicalId":20692,"journal":{"name":"Proceedings of the YSU B: Chemical and Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81791255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-12-23DOI: 10.46991/pysu:b/2021.55.3.296
V. Kirakosyan, S. M. Vardapetyan, Lusine H. Varderesyan, A. Tsaturyan
In this study, chromatographic conditions were developed for the separation and quantification of N-nitrosodimethylamine (NDMA) by HPLC. Our method would be useful for the rapid screening and quantification of NDMA impurity in Metformin hydrochloride, Losartan potassium, Valsartan and Ranitidine drugs substance and its products. The chromatographic separation was achieved on a Phenomenex C18 column (250 mm, 5 µm, 4.6 mm) by multistep gradient elution, using a water-acetonitrile mobile phase containing 0.1% formic acid, which provided better c hromatographic separation. Column temperature was maintained at 30℃; mobile phase flow rate: 1 mL/min; wavelength: 254 nm; and injection volume of 20 μL.
{"title":"DEVELOPMENT OF A RAPID AND EFFICIENT METHOD FOR QUANTITATIVE DETERMINATION OF N-NITROSODIMETHYLAMINE IMPURITY BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY IN METFORMIN HYDROCHLORIDE, LOSARTAN POTASSIUM, VALSARTAN AND RANITIDINE MEDICINAL RAW MATERIALS AND ITS PRODUCTS","authors":"V. Kirakosyan, S. M. Vardapetyan, Lusine H. Varderesyan, A. Tsaturyan","doi":"10.46991/pysu:b/2021.55.3.296","DOIUrl":"https://doi.org/10.46991/pysu:b/2021.55.3.296","url":null,"abstract":"In this study, chromatographic conditions were developed for the separation and quantification of N-nitrosodimethylamine (NDMA) by HPLC. Our method would be useful for the rapid screening and quantification of NDMA impurity in Metformin hydrochloride, Losartan potassium, Valsartan and Ranitidine drugs substance and its products. The chromatographic separation was achieved on a Phenomenex C18 column (250 mm, 5 µm, 4.6 mm) by multistep gradient elution, using a water-acetonitrile mobile phase containing 0.1% formic acid, which provided better c hromatographic separation. Column temperature was maintained at 30℃; mobile phase flow rate: 1 mL/min; wavelength: 254 nm; and injection volume of 20 μL.","PeriodicalId":20692,"journal":{"name":"Proceedings of the YSU B: Chemical and Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90388738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-12-23DOI: 10.46991/pysu:b/2021.55.3.290
Gajane H. Semerjyan, Inessa H. Semerjyan
Bryophytes are rich in a variety of secondary biological active compounds, thus provide a great potential for biotechnological and biopharmaceutical applications. Bryophytes contain potentially useful natural products, including polysaccharides terpenoids, lipids, amino acids and phenylpropanoids. Bryophytes isolated compounds and extracts have cytotoxic, antimicrobial activities. The result of our research indicate the presence in the extracts of bryophytes collected in Armenia, the entire spectrum of amino acids, especially essential ones. Also revealed in some species a certain amount of nitrogen – 840 mg/g, which indicates a more intensive synthesis of nonessential amino acids. The results obtained can be used in medicine, pharmacology.
{"title":"INVESTIGATION OF AMINO ACID COMPOSITION OF SOME MOSSES GATHERED FROM ARMENIA","authors":"Gajane H. Semerjyan, Inessa H. Semerjyan","doi":"10.46991/pysu:b/2021.55.3.290","DOIUrl":"https://doi.org/10.46991/pysu:b/2021.55.3.290","url":null,"abstract":"Bryophytes are rich in a variety of secondary biological active compounds, thus provide a great potential for biotechnological and biopharmaceutical applications. Bryophytes contain potentially useful natural products, including polysaccharides terpenoids, lipids, amino acids and phenylpropanoids. Bryophytes isolated compounds and extracts have cytotoxic, antimicrobial activities. The result of our research indicate the presence in the extracts of bryophytes collected in Armenia, the entire spectrum of amino acids, especially essential ones. Also revealed in some species a certain amount of nitrogen – 840 mg/g, which indicates a more intensive synthesis of nonessential amino acids. The results obtained can be used in medicine, pharmacology.","PeriodicalId":20692,"journal":{"name":"Proceedings of the YSU B: Chemical and Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80355858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-12-23DOI: 10.46991/pysu:b/2021.55.3.248
Meri Iskandaryan, E. Barseghyan
There are some aspects of the proteins «self-organization» that are not well studied, understanding the relationship between conformational folding linked with the formation of a disulfide bond is important and challenging both from a biophysical and a biochemical perspectives. Studies have attempted to elucidate the role of thiol groups in the maintenance of native conformation and activity of the arginase I and II with a different organ origin. It was identified that depending on the stage of enzyme reactivation and the stability of the conformational state of the formed oligomers, the para-chloromercuribenzoate affects the «self-organised» oligomers in different ways.
{"title":"THE ROLE OF THIOL GROUPS IN THE EXPRESSION OF THE ACTIVITY OF ARGINASE I AND II ISOENZYMES","authors":"Meri Iskandaryan, E. Barseghyan","doi":"10.46991/pysu:b/2021.55.3.248","DOIUrl":"https://doi.org/10.46991/pysu:b/2021.55.3.248","url":null,"abstract":"There are some aspects of the proteins «self-organization» that are not well studied, understanding the relationship between conformational folding linked with the formation of a disulfide bond is important and challenging both from a biophysical and a biochemical perspectives. Studies have attempted to elucidate the role of thiol groups in the maintenance of native conformation and activity of the arginase I and II with a different organ origin. It was identified that depending on the stage of enzyme reactivation and the stability of the conformational state of the formed oligomers, the para-chloromercuribenzoate affects the «self-organised» oligomers in different ways.","PeriodicalId":20692,"journal":{"name":"Proceedings of the YSU B: Chemical and Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76416897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-12-23DOI: 10.46991/pysu:b/2021.55.3.224
S. Blbulyan, Satenik N. Mirzoyan, K. Trchounian, A. Poladyan
Physicochemical pretreatment of lignocellulosic corn fruit wastes (husk, shank, stalk) has been done. E. coli K12 growth, ORP kinetics, and H2 production were followed upon utilization of corn waste hydrolysates (CWH) separately and their mixture. During the bacterial log growth phase with the decrease of medium pH the readings of Pt electrode from positive to low negative values (~ –450±10 mV) were observed, with the yield ~ 0.75 mmol H2 /L and 0.29 bacterial cell dry weight g/L biomass in CWH based medium. Meat (chicken wastes) broth supplementation ~2 fold enhanced both E. coli biomass formation and H2 production on CWH mixture.
{"title":"BIOMASS AND BIOHYDROGEN PRODUCTION BY $ESCHERICHIA~COLI$ UPON CONSUMPTION OF MEAT INDUSTRY AND LIGNOCELLULOSIC CORN WASTES MIXTURE","authors":"S. Blbulyan, Satenik N. Mirzoyan, K. Trchounian, A. Poladyan","doi":"10.46991/pysu:b/2021.55.3.224","DOIUrl":"https://doi.org/10.46991/pysu:b/2021.55.3.224","url":null,"abstract":"Physicochemical pretreatment of lignocellulosic corn fruit wastes (husk, shank, stalk) has been done. E. coli K12 growth, ORP kinetics, and H2 production were followed upon utilization of corn waste hydrolysates (CWH) separately and their mixture. During the bacterial log growth phase with the decrease of medium pH the readings of Pt electrode from positive to low negative values (~ –450±10 mV) were observed, with the yield ~ 0.75 mmol H2 /L and 0.29 bacterial cell dry weight g/L biomass in CWH based medium. Meat (chicken wastes) broth supplementation ~2 fold enhanced both E. coli biomass formation and H2 production on CWH mixture.","PeriodicalId":20692,"journal":{"name":"Proceedings of the YSU B: Chemical and Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75256434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-12-23DOI: 10.46991/pysu:b/2021.55.3.204
S. G. Petrosyan, L. Sahakyan, V. Kirakosyan, Ella V. Minasyan, Siranush Ye. Sardaryan
The optimal conditions of the method, applicable during forensic chemical examinations, were elaborated. Particularly, the investigation of the conditions for the isolation of Nifedipine from liver and skeletal muscle tissues, the identification of the isolated Nifedipine by TLC method and checking the accuracy of the method using HPLC was carried out. As a result, a new alternative express method for the isolation and further identification of Nifedipine from different biomaterials was elaborated. The method is quite fast, accurate and much cheaper.
{"title":"ISOLATION, IDENTIFICATION AND QUANTITATIVE DETERMINATION OF NIFEDIPINE FROM BIOLOGICAL MATERIAL","authors":"S. G. Petrosyan, L. Sahakyan, V. Kirakosyan, Ella V. Minasyan, Siranush Ye. Sardaryan","doi":"10.46991/pysu:b/2021.55.3.204","DOIUrl":"https://doi.org/10.46991/pysu:b/2021.55.3.204","url":null,"abstract":"The optimal conditions of the method, applicable during forensic chemical examinations, were elaborated. Particularly, the investigation of the conditions for the isolation of Nifedipine from liver and skeletal muscle tissues, the identification of the isolated Nifedipine by TLC method and checking the accuracy of the method using HPLC was carried out. As a result, a new alternative express method for the isolation and further identification of Nifedipine from different biomaterials was elaborated. The method is quite fast, accurate and much cheaper.","PeriodicalId":20692,"journal":{"name":"Proceedings of the YSU B: Chemical and Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85746278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-10-15DOI: 10.46991/pysu:b/2021.55.3.199
M. L. Yeritsyan, Аrmen M. Аrustamyan, V. Mavisakalyan, Inessa M. Sirekanyan, S. Tsarukyan
The interaction of N-monomethylol urea with maleic anhydride is studied. The product of this reaction forms an oligomeric monomer by condensation with chitosan. This oligomeric monomer has a capacity of copolymerization in aqueous media with other soluble monomers such as acrylic acid forming crosslinked water-swelling copolymer. The products obtained in mentioned above reactions were investigated by IR-spectroscopy and elemental analysis.
{"title":"MODIFICATION OF CHITOSAN BY GRAFT COPOLYMERIZATION","authors":"M. L. Yeritsyan, Аrmen M. Аrustamyan, V. Mavisakalyan, Inessa M. Sirekanyan, S. Tsarukyan","doi":"10.46991/pysu:b/2021.55.3.199","DOIUrl":"https://doi.org/10.46991/pysu:b/2021.55.3.199","url":null,"abstract":"The interaction of N-monomethylol urea with maleic anhydride is studied. The product of this reaction forms an oligomeric monomer by condensation with chitosan. This oligomeric monomer has a capacity of copolymerization in aqueous media with other soluble monomers such as acrylic acid forming crosslinked water-swelling copolymer. The products obtained in mentioned above reactions were investigated by IR-spectroscopy and elemental analysis.","PeriodicalId":20692,"journal":{"name":"Proceedings of the YSU B: Chemical and Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79501916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-08-30DOI: 10.46991/pysu:b/2021.55.2.125
S. Hayrapetyan, M. S. Hayrapetyan, A. I. Martiryan, H. H. Darbinyan, H. H. Khachatryan
The sorption properties of organomineral composite sorbents have been investigated by ICP-MS method. The diatomite of the Jradzor deposit and bentonite of the Sarigyuh deposit (Armenia) were used as inorganic constituents. Diatomite serves as a structure-forming component and provides mass transfer due to its high porosity (V = 2.0 cm3/g) and large pore sizes (150–200 nm). Bentonite is responsible for the functionality of the sorbents. The organic component is also responsible for the functionality of the obtained sorbents. Paper pretreated with acids (nitric and phosphoric) was used as an organic component. A synthetic solution containing cations of heavy metals was used as an object of study. The experiments were carried out in static mode on a Jar-Test device.
{"title":"COMPOSITE SORBENTS BASED ON DIATOMITE-BENTONITE AND PAPER-PULP FOR THE REMOVAL OF HEAVY METALS","authors":"S. Hayrapetyan, M. S. Hayrapetyan, A. I. Martiryan, H. H. Darbinyan, H. H. Khachatryan","doi":"10.46991/pysu:b/2021.55.2.125","DOIUrl":"https://doi.org/10.46991/pysu:b/2021.55.2.125","url":null,"abstract":"The sorption properties of organomineral composite sorbents have been investigated by ICP-MS method. The diatomite of the Jradzor deposit and bentonite of the Sarigyuh deposit (Armenia) were used as inorganic constituents. Diatomite serves as a structure-forming component and provides mass transfer due to its high porosity (V = 2.0 cm3/g) and large pore sizes (150–200 nm). Bentonite is responsible for the functionality of the sorbents. The organic component is also responsible for the functionality of the obtained sorbents. Paper pretreated with acids (nitric and phosphoric) was used as an organic component. A synthetic solution containing cations of heavy metals was used as an object of study. The experiments were carried out in static mode on a Jar-Test device.","PeriodicalId":20692,"journal":{"name":"Proceedings of the YSU B: Chemical and Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89081927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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