Pub Date : 2021-01-01DOI: 10.46991/pysu:b/2021.55.2.158
N. Petrosyan
The study on the interaction of DNA-specific low-molecular compounds – groove binding material Hoechst 33258 and intercalating ligand methylene blue (MB) with serum albumin has been carried out. The absorption and differential absorption spectra of complexes of the mentioned ligands with protein were obtained. Changes of the absorption and differential absorption spectra indicate the binding of two ligands with albumin. The obtained results indicate that at the interaction with both ligands, the conformational state of the protein alters, though these changes are not similar, since in the case of MB a compactization of the protein folding occurs, while in the case of Hoechst 33258, most apparently, an unfolding of the compact structure takes place as a result of partial loss of helicity of $alpha$-structures.
{"title":"STUDY OF ABSORPTION SPECTRA OF THE COMPLEXES OF BOVINE SERUM ALBUMIN WITH HOECHST 33258 AND METHYLENE BLUE","authors":"N. Petrosyan","doi":"10.46991/pysu:b/2021.55.2.158","DOIUrl":"https://doi.org/10.46991/pysu:b/2021.55.2.158","url":null,"abstract":"The study on the interaction of DNA-specific low-molecular compounds – groove binding material Hoechst 33258 and intercalating ligand methylene blue (MB) with serum albumin has been carried out. The absorption and differential absorption spectra of complexes of the mentioned ligands with protein were obtained. Changes of the absorption and differential absorption spectra indicate the binding of two ligands with albumin. The obtained results indicate that at the interaction with both ligands, the conformational state of the protein alters, though these changes are not similar, since in the case of MB a compactization of the protein folding occurs, while in the case of Hoechst 33258, most apparently, an unfolding of the compact structure takes place as a result of partial loss of helicity of $alpha$-structures.","PeriodicalId":20692,"journal":{"name":"Proceedings of the YSU B: Chemical and Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75078647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.46991/pysu:b/2021.55.2.165
L. Minasbekyan, I. A. Avagyan
The study of the effect of different doses of $gamma$-irradiation on the growth and development of seedlings of soft wheat seeds, resistance, and germination of seeds to irradiation has been carried out. The data show that the seeds of common wheat are resistant to ionizing radiation and up to 80% of the seeds remained viable. Under the influence of ionizing radiation, the functional and mitotic activity of the cell nucleus is disrupted, which has a significant effect on the growth parameters of the seedlings of irradiated seeds.
{"title":"RADIOSENSITIVITY OF SOFT WHEAT SEEDS TO GAMMA RADIATION","authors":"L. Minasbekyan, I. A. Avagyan","doi":"10.46991/pysu:b/2021.55.2.165","DOIUrl":"https://doi.org/10.46991/pysu:b/2021.55.2.165","url":null,"abstract":"The study of the effect of different doses of $gamma$-irradiation on the growth and development of seedlings of soft wheat seeds, resistance, and germination of seeds to irradiation has been carried out. The data show that the seeds of common wheat are resistant to ionizing radiation and up to 80% of the seeds remained viable. Under the influence of ionizing radiation, the functional and mitotic activity of the cell nucleus is disrupted, which has a significant effect on the growth parameters of the seedlings of irradiated seeds.","PeriodicalId":20692,"journal":{"name":"Proceedings of the YSU B: Chemical and Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76044558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.46991/pysu:b/2021.55.1.118
G. S. Grigoryan
The formation of Ni2+:Tryptophan (Trp) 1:1 complex, which acts as a model catalyst for decomposition of cumene hydroperoxide (ROOH) in Ni2++Trp+ROOH+H2O system, has been confirmed via kinetic study in aqueous solution at pH>7. The kinetic expression of a single catalytic decomposition reaction of ROOH under the influence of [NiTrp]+ complex was brought out. The temperature dependence of the effective rate constant of ROOH decay (Keff=Kcat[Ni2+]0[Trp]0=const) in the temperature range from 323 to 343 K can be expressed by Arrhenius equation (Eeff is in kJ/mol): Keff=(1.87±0.02)·106exp[–(49.8±0.3)/RT], min –1.
在pH>7的水溶液中，通过动力学研究证实了Ni2++Trp+ROOH+H2O体系中形成Ni2+:色氨酸(Trp) 1:1配合物，作为异丙苯过氧化氢(ROOH)分解的模型催化剂。给出了[NiTrp]+配合物作用下ROOH单催化分解反应的动力学表达式。在323 ~ 343 K范围内，ROOH衰变的有效速率常数(Keff=Kcat[Ni2+]0[Trp]0=const)与温度的关系可以用Arrhenius方程表示(Eeff单位为kJ/mol): Keff=(1.87±0.02)·106exp[-(49.8±0.3)/RT]， min - 1。
{"title":"COMPLEX OF NICKEL (II) ION WITH TRYPTOPHAN AS A HOMOGENEOUS CATALYST IN THE DECOMPOSITION REACTION OF CUMENE HYDROPEROXIDE IN AQUEOUS SOLUTION","authors":"G. S. Grigoryan","doi":"10.46991/pysu:b/2021.55.1.118","DOIUrl":"https://doi.org/10.46991/pysu:b/2021.55.1.118","url":null,"abstract":"The formation of Ni2+:Tryptophan (Trp) 1:1 complex, which acts as a model catalyst for decomposition of cumene hydroperoxide (ROOH) in Ni2++Trp+ROOH+H2O system, has been confirmed via kinetic study in aqueous solution at pH>7. The kinetic expression of a single catalytic decomposition reaction of ROOH under the influence of [NiTrp]+ complex was brought out. The temperature dependence of the effective rate constant of ROOH decay (Keff=Kcat[Ni2+]0[Trp]0=const) in the temperature range from 323 to 343 K can be expressed by Arrhenius equation (Eeff is in kJ/mol): Keff=(1.87±0.02)·106exp[–(49.8±0.3)/RT], min –1.","PeriodicalId":20692,"journal":{"name":"Proceedings of the YSU B: Chemical and Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90942636","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-12-28DOI: 10.46991/pysu:b/2020.54.3.188
A. I. Martiryan, A. Galstyan, L. Tadevosyan, I. A. Petrosyan
On the basis of cyclic esters а method for producing of γ-hydroxybutyric acids hydrazides has been elaborated. The antioxidant properties of hydrazides were researched by the method of competitive reactions and it was established that they have antioxidant activity.
{"title":"SYNTHESIS OF γ-HYDROXY ACID HYDRAZIDES OF A NEW STRUCTURE AND STUDY OF THEIR ANTIOXIDANT PROPERTIES","authors":"A. I. Martiryan, A. Galstyan, L. Tadevosyan, I. A. Petrosyan","doi":"10.46991/pysu:b/2020.54.3.188","DOIUrl":"https://doi.org/10.46991/pysu:b/2020.54.3.188","url":null,"abstract":"On the basis of cyclic esters а method for producing of γ-hydroxybutyric acids hydrazides has been elaborated. The antioxidant properties of hydrazides were researched by the method of competitive reactions and it was established that they have antioxidant activity.","PeriodicalId":20692,"journal":{"name":"Proceedings of the YSU B: Chemical and Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84647367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-12-15DOI: 10.46991/pysu:b/2020.54.3.216
N. Sahakyan, M. Petrosyan, A. Trchounian
Cell redox homeostasis is formed as a result of the balance between the accumulation of reactive oxygen species and functioning of the antioxidant enzymes or non-enzymatic antioxidants. The alcohol extracts from the leaves of Ribes nigrum L. were prepared to investigate the antioxidant activity and protective effects against both BV-2 microglial wild type (WT) cells and acyl-CoA oxidase type 1 (ACOX1) deficient cell (Acox1–/–) lines․ Our investigations showed that R. nigrum extracts possess remarkable antiradical activity in 1-diphenyl-2-picrylhydrazyl (DPPH) with IC50 value of 32.7±0.8 µg mL–1. 3-(4,5-dimethyltrazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay showed that the sub-cytotoxic concentration of R. nigrum extract in both cases was 1 mg mL–1 The further treatment of microglial cells with the mentioned concentration of extract brought the remarkable increasing (up to 350%) of activity of the total superoxide dismutase (SOD) – the core antioxidant enzymes, under the treatment during the 48–72 h. So, relaying on the obtained data it is possible to insist that the lack of ACOX1 can be compensated by the activation of cell enzymatic antioxidant defense mechanisms.
{"title":"INCREASING OF THE SUPEROXIDE DISMUTASE TOTAL ACTIVITY IN MICROGLIAL CELLS UNDER THE TREATMENT BY $RIBES$ $NIGRUM$ L. ALCOHOL EXTRACT","authors":"N. Sahakyan, M. Petrosyan, A. Trchounian","doi":"10.46991/pysu:b/2020.54.3.216","DOIUrl":"https://doi.org/10.46991/pysu:b/2020.54.3.216","url":null,"abstract":"Cell redox homeostasis is formed as a result of the balance between the accumulation of reactive oxygen species and functioning of the antioxidant enzymes or non-enzymatic antioxidants. The alcohol extracts from the leaves of Ribes nigrum L. were prepared to investigate the antioxidant activity and protective effects against both BV-2 microglial wild type (WT) cells and acyl-CoA oxidase type 1 (ACOX1) deficient cell (Acox1–/–) lines․ Our investigations showed that R. nigrum extracts possess remarkable antiradical activity in 1-diphenyl-2-picrylhydrazyl (DPPH) with IC50 value of 32.7±0.8 µg mL–1. 3-(4,5-dimethyltrazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay showed that the sub-cytotoxic concentration of R. nigrum extract in both cases was 1 mg mL–1 The further treatment of microglial cells with the mentioned concentration of extract brought the remarkable increasing (up to 350%) of activity of the total superoxide dismutase (SOD) – the core antioxidant enzymes, under the treatment during the 48–72 h. So, relaying on the obtained data it is possible to insist that the lack of ACOX1 can be compensated by the activation of cell enzymatic antioxidant defense mechanisms.","PeriodicalId":20692,"journal":{"name":"Proceedings of the YSU B: Chemical and Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79475793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-12-15DOI: 10.46991/pysu:b/2020.54.3.246
L. Hambaryan
An assessment of the ecological state of the Voghji River and its catchment basin according to chemical and biological indicators in the period 2018–2019. New taxa were discovered in phytoplankton, when contaminated with heavy metals and organic substances. A decrease in community stability and an increase in the role of Сyanoprokaryotes and green algae in plankton were observed, which can be used for phytoremediation of the degraded ecosystem of the Voghji River.
{"title":"PHYTOPLANKTON FORMATION UNDER CONDITIONS OF DIFFERENT TYPES OF POLLUTION AND CHANGES IN THE WATER QUALITY OF THE VOGHJI RIVER","authors":"L. Hambaryan","doi":"10.46991/pysu:b/2020.54.3.246","DOIUrl":"https://doi.org/10.46991/pysu:b/2020.54.3.246","url":null,"abstract":"An assessment of the ecological state of the Voghji River and its catchment basin according to chemical and biological indicators in the period 2018–2019. New taxa were discovered in phytoplankton, when contaminated with heavy metals and organic substances. A decrease in community stability and an increase in the role of Сyanoprokaryotes and green algae in plankton were observed, which can be used for phytoremediation of the degraded ecosystem of the Voghji River.","PeriodicalId":20692,"journal":{"name":"Proceedings of the YSU B: Chemical and Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82338950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-12-15DOI: 10.46991/PYSU:B/2020.54.3.204
P. O. Vardevanyan, M. Mikaelyan, N. Petrosyan
The interaction of Hoechst 33258 (H33258) and methylene blue (MB) compounds with bovine serum albumin (BSA) has been studied using the method of thermal denaturation. The obtained data showed that both ligands form complexes with BSA, moreover, MB binds to BSA stronger than H33258. Furthermore, H33258 destabilizes, while MB stabilizes the native structure of protein, leading to the decrease and increase of the denaturation temperature of BSA respectively.
{"title":"BOVINE SERUM ALBUMIN DENATURATION IN THE PRESENCE OF HOECHST 33258 AND METHYLENE BLUE","authors":"P. O. Vardevanyan, M. Mikaelyan, N. Petrosyan","doi":"10.46991/PYSU:B/2020.54.3.204","DOIUrl":"https://doi.org/10.46991/PYSU:B/2020.54.3.204","url":null,"abstract":"The interaction of Hoechst 33258 (H33258) and methylene blue (MB) compounds with bovine serum albumin (BSA) has been studied using the method of thermal denaturation. The obtained data showed that both ligands form complexes with BSA, moreover, MB binds to BSA stronger than H33258. Furthermore, H33258 destabilizes, while MB stabilizes the native structure of protein, leading to the decrease and increase of the denaturation temperature of BSA respectively.","PeriodicalId":20692,"journal":{"name":"Proceedings of the YSU B: Chemical and Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75737664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-12-15DOI: 10.46991/pysu:b/2020.54.3.223
M. Karapetyan, S. Sahakyan, N. Adamyan
Using the “Bioscope” hardware complex, it was shown that an oxygen deficiency leads to certain changes in the statistical parameters of the integrative state of animals both during and after hypoxia exposure. However, after 10 days of using horseradish roots (Armoracia rusticana) as an antihypoxic food supplement, in contrast to the control, significant changes in the recorded signal are observed both during and after hypoxic exposure. These changes indicate the facilitating effect of horseradish roots in conditions of oxygen deficiency.
{"title":"THE IMPACT OF HORSERADISH ROOTS ($ARMORACIA$ $RUSTICANA$) ON THE FUNCTIONAL STATE OF RAT BODY IN ACUTE HYPOXIA","authors":"M. Karapetyan, S. Sahakyan, N. Adamyan","doi":"10.46991/pysu:b/2020.54.3.223","DOIUrl":"https://doi.org/10.46991/pysu:b/2020.54.3.223","url":null,"abstract":"Using the “Bioscope” hardware complex, it was shown that an oxygen deficiency leads to certain changes in the statistical parameters of the integrative state of animals both during and after hypoxia exposure. However, after 10 days of using horseradish roots (Armoracia rusticana) as an antihypoxic food supplement, in contrast to the control, significant changes in the recorded signal are observed both during and after hypoxic exposure. These changes indicate the facilitating effect of horseradish roots in conditions of oxygen deficiency.","PeriodicalId":20692,"journal":{"name":"Proceedings of the YSU B: Chemical and Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75966886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-12-15DOI: 10.46991/PYSU:B/2020.54.3.235
K. Ghazaryan, H. Movsesyan
The aim of this study was to define a relationship between heavy metal (Cu, Mo) pollution of soil and various extracellular enzyme activities. Six enzymatic activities involved in cycles of carbon, nitrogen, phosphorus and sulfur (β-glucosidase, chitinase, leucine-aminopeptidase, acid phosphomonoesterase, alkaline phosphomonoesterase, and arylsulphatase) as well as microbial biomass were determined in soil samples collected in the surroundings of Zangezur Copper and Molybdenum Combine. The investigations showed that pollution of soil with copper and molybdenum led to a decrease in microbial biomass and soil enzymatic activity, which in turn had a negative impact on cycles of chemical elements, in particular C, P, N and S. This gives reason to conclude that the changes in soil microbial biomass and enzymatic activity may act as indicators of soil biological activity and quality.
{"title":"SOIL MICROBIAL BIOMASS AND ENZYME ACTIVITIES RESPONSE TO COPPER AND MOLYBDENUM POLLUTION IN THE VICINITY OF ZANGEZUR COPPER AND MOLYBDENUM COMBINE, ARMENIA","authors":"K. Ghazaryan, H. Movsesyan","doi":"10.46991/PYSU:B/2020.54.3.235","DOIUrl":"https://doi.org/10.46991/PYSU:B/2020.54.3.235","url":null,"abstract":"The aim of this study was to define a relationship between heavy metal (Cu, Mo) pollution of soil and various extracellular enzyme activities. Six enzymatic activities involved in cycles of carbon, nitrogen, phosphorus and sulfur (β-glucosidase, chitinase, leucine-aminopeptidase, acid phosphomonoesterase, alkaline phosphomonoesterase, and arylsulphatase) as well as microbial biomass were determined in soil samples collected in the surroundings of Zangezur Copper and Molybdenum Combine. The investigations showed that pollution of soil with copper and molybdenum led to a decrease in microbial biomass and soil enzymatic activity, which in turn had a negative impact on cycles of chemical elements, in particular C, P, N and S. This gives reason to conclude that the changes in soil microbial biomass and enzymatic activity may act as indicators of soil biological activity and quality.","PeriodicalId":20692,"journal":{"name":"Proceedings of the YSU B: Chemical and Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86572876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-12-15DOI: 10.46991/pysu:b/2020.54.3.209
M. Shahinyan, M. Mikaelyan, A. Nerkararyan
The effect of millimeter range electromagnetic waves (MM EMW) on kinetics of acidic hemolysis of rat blood erythrocytes has been studied. It was shown that MM EMW affect the duration of erythrocyte hemolysis and this change depends on MM EMW exposure and frequency. At the irradiation by frequencies 41.8 and 42.2 GHz the acidic hemolysis occurs quicker at all durations of the irradiation compared to the control. At the irradiation by 50.3 and 51.8 GHz the hemolysis takes place quicker as well, as compared to the control at short durations of the irradiation. At the exposure 60 min the hemolysis start is delayed.
{"title":"INFLUENCE OF MILLIMETER RANGE ELECTROMAGNETIC WAVES ON HEMOLYSIS KINETICS OF RAT BLOOD ERYTHROCYTES","authors":"M. Shahinyan, M. Mikaelyan, A. Nerkararyan","doi":"10.46991/pysu:b/2020.54.3.209","DOIUrl":"https://doi.org/10.46991/pysu:b/2020.54.3.209","url":null,"abstract":"The effect of millimeter range electromagnetic waves (MM EMW) on kinetics of acidic hemolysis of rat blood erythrocytes has been studied. It was shown that MM EMW affect the duration of erythrocyte hemolysis and this change depends on MM EMW exposure and frequency. At the irradiation by frequencies 41.8 and 42.2 GHz the acidic hemolysis occurs quicker at all durations of the irradiation compared to the control. At the irradiation by 50.3 and 51.8 GHz the hemolysis takes place quicker as well, as compared to the control at short durations of the irradiation. At the exposure 60 min the hemolysis start is delayed.","PeriodicalId":20692,"journal":{"name":"Proceedings of the YSU B: Chemical and Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72663294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：