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Assessing the reporting of harms in systematic reviews focused on platelet-rich plasma therapy: a cross-sectional analysis. 评估富血小板血浆治疗系统评价中危害报告:一项横断面分析
IF 2.7 4区 医学 Q1 Medicine Pub Date : 2023-07-01 DOI: 10.2217/rme-2023-0058
Shaelyn Ward, Kade Ezell, Audrey Wise, Morgan Garrett, Brayden Rucker, Drew Lestersmith, Mohammed Emam, Matt Vassar

Aim: Our objectives are: to evaluate the completeness of harms reporting in systematic reviews (SRs) on platelet-rich plasma therapy; to assess the overall methodological quality of the SR using AMSTAR-2 tool; to assess harms reporting overlap in primary studies between SRs. Materials & methods: The authors searched five database systems for relevant literature on platelet rich plasma therapy. The authors screened and extracted in masked, duplicate fashion. Results: All SRs reported less than 50% completeness in harms reporting. The most frequently reported item was harms being stated in the abstract or title (26/103, 25.2%). AMSTAR-2 assessed 96 SRs as 'critically low', 6 SRs as 'low' and 1 'moderate'. Conclusion: Our study highlights that reporting of harms should become more standardized and transparent.

目的:我们的目标是:评估富血小板血浆治疗系统评价(SRs)中危害报告的完整性;使用AMSTAR-2工具评估SR的整体方法质量;评估初级研究中危害报告的重叠。材料与方法:在5个数据库系统中检索富血小板血浆治疗的相关文献。作者筛选和提取蒙面,重复的方式。结果:所有SRs报告的危害报告完整性均低于50%。报告次数最多的项目是摘要或标题中所述的危害(26/103,25.2%)。AMSTAR-2将96个sr评估为“极低”,6个为“低”,1个为“中等”。结论:我们的研究强调了危害报告应该变得更加标准化和透明。
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引用次数: 0
Comprehensive analysis of culture conditions governing differentiation of MSCs into articular chondrocytes. 骨髓间充质干细胞向关节软骨细胞分化的培养条件综合分析。
IF 2.7 4区 医学 Q1 Medicine Pub Date : 2023-07-01 DOI: 10.2217/rme-2023-0017
Harsh Vikram Singh, Lakshmana Das, Rhuthuparna Malayil, Tashvinder Singh, Sandeep Singh, Tarun Goyal, Anjana Munshi

Treatment of osteoarthritic patients requires the development of morphologically and mechanically complex hyaline cartilage at the injury site. A tissue engineering approach toward differentiating mesenchymal stem cells into articular chondrocytes has been developed to overcome the drawbacks of conventional therapeutic and surgical procedures. To imitate the native micro and macro environment of articular chondrocytes, cell culture parameters such as oxygen concentration, mechanical stress, scaffold design, and growth factor signalling cascade regulation must be addressed. This review aims to illuminate the path toward developing tissue engineering approaches, accommodating these various parameters and the role these parameters play in regulating chondrogenesis for better articular cartilage development to treat osteoarthritis effectively.

骨关节炎患者的治疗需要在损伤部位形成形态和机械复杂的透明软骨。一种将间充质干细胞分化为关节软骨细胞的组织工程方法已经被开发出来,以克服传统治疗和外科手术的缺点。为了模拟关节软骨细胞的原生微观和宏观环境,必须解决细胞培养参数,如氧浓度、机械应力、支架设计和生长因子信号级联调节。本文旨在阐明组织工程方法的发展路径,适应这些不同的参数以及这些参数在调节软骨形成中的作用,以更好地发育关节软骨,有效地治疗骨关节炎。
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引用次数: 0
Astragaloside-IV induces the differentiation of bone marrow mesenchymal stem cells into osteoblasts through NMUR2-mediated Wnt/β-catenin pathway. 黄芪甲苷通过nmur2介导的Wnt/β-catenin通路诱导骨髓间充质干细胞向成骨细胞分化。
IF 2.7 4区 医学 Q1 Medicine Pub Date : 2023-06-01 DOI: 10.2217/rme-2022-0184
Yujing Cao, Qiuxia Lv, Zhihui Huang, Yang Li

Aim: Given that astragaloside-IV (As-IV) has the ability to promote osteogenic differentiation, its mechanism is worthy of exploration. Methods: The effect of As-IV on rat tibial defects was examined by histopathological staining and MiR-CT scan. The alkaline phosphatase (ALP) content, osteogenic differentiation-related gene expressions, and mineralized nodule formation in bone marrow mesenchymal stem cells (BMSCs) were detected. Results: As-IV repaired tibial defects of rats. As-IV or neuromedin receptor 2 (NMUR2) overexpression elevated ALP content, mineralized nodules, osteogenic differentiation-related genes, β-catenin and NMUR2 levels, the effects of which were reversed by NMUR2 silencing or Wnt/β-catenin pathway inhibitors. Conclusion: As-IV regulates the Wnt/β-catenin pathway through NMUR2 to promote the repair of tibial defects in rats and the differentiation of BMSCs into osteoblasts.

目的:黄芪甲苷(astragaloside-IV, As-IV)具有促进成骨分化的能力,其机制值得探讨。方法:采用组织病理学染色和MiR-CT扫描检测As-IV对大鼠胫骨缺损的影响。检测骨髓间充质干细胞(BMSCs)碱性磷酸酶(ALP)含量、成骨分化相关基因表达及矿化结节形成。结果:As-IV修复大鼠胫骨缺损。As-IV或神经medin受体2 (NMUR2)过表达升高ALP含量、矿化结节、成骨分化相关基因、β-catenin和NMUR2水平,这些作用可通过NMUR2沉默或Wnt/β-catenin途径抑制剂逆转。结论:As-IV通过NMUR2调控Wnt/β-catenin通路,促进大鼠胫骨缺损修复及BMSCs向成骨细胞分化。
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引用次数: 0
Pseudoseptic reaction to an intra-articular platelet-rich plasma injection into the knee: a case report. 膝关节内关节内富血小板血浆注射的假性化脓性反应:1例报告。
IF 2.7 4区 医学 Q1 Medicine Pub Date : 2023-06-01 DOI: 10.2217/rme-2022-0216
Kyungje Sung, Kevin Zheng, Chris Williams, David Cunningham, Walter I Sussman

A variety of intra-articular injections are used for the management of osteoarthritis. A rare complication of intra-articular injections is acute pseudoseptic arthritis, which mimics true septic arthritis. To the authors knowledge, pseudosepsis has not been reported as a complication after platelet-rich plasma (PRP) injections. We present a case report of pseudoseptic arthritis resulting in acute postinjection pain and a joint effusion after an intra-articular PRP injection into the knee. Clinically, pseudosepsis can occur after PRP treatment with significantly elevated white blood cell counts in the synovial fluid, and should be a consideration in cases of post-PRP injection flares.

各种关节内注射用于骨关节炎的治疗。关节内注射的一个罕见的并发症是急性假性脓毒性关节炎,它模仿真正的脓毒性关节炎。据作者所知,假性败血症尚未报道为富血小板血浆(PRP)注射后的并发症。我们提出一个病例报告的假性化脓性关节炎导致急性注射后疼痛和关节积液后,关节内PRP注射到膝盖。临床上,假性脓毒症可发生在PRP治疗后,滑液中白细胞计数明显升高,在PRP注射后出现耀斑的情况下应予以考虑。
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引用次数: 0
Industry updates from the field of stem cell research and regenerative medicine in February 2023. 2023年2月来自干细胞研究和再生医学领域的行业更新。
IF 2.7 4区 医学 Q1 Medicine Pub Date : 2023-06-01 DOI: 10.2217/rme-2023-0053
Dusko Ilic, Mirjana Liovic
Latest developments in the field of stem cell research and regenerative medicine compiled from publicly available information and press releases from non-academic institutions in February 2023.
根据公开信息和非学术机构的新闻稿汇编的干细胞研究和再生医学领域的最新进展。
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引用次数: 0
Derivation of a clinical decision rule for a bone marrow aspirate concentrate injection in knee osteoarthritis. 骨髓浓缩液注射治疗膝骨关节炎的临床决策规则的推导。
IF 2.7 4区 医学 Q1 Medicine Pub Date : 2023-06-01 DOI: 10.2217/rme-2023-0014
Ashley D Smith, Dasan Sydora, Robert Burnham

Aim: To develop a simple clinical decision rule (CDR) to identify people with knee osteoarthritis who are likely or unlikely to benefit from bone marrow aspirate concentrate (BMAC) injection. Materials & methods: A total of 92 people with clinical and radiographic evidence of refractory knee osteoarthritis received a single intra-articular (IA) BMAC injection. Multiple logistic regression analysis was used to determine which combination of risk factors predicted BMAC responsiveness. A responder was defined as a person whose knee pain improved more than 15% from baseline 6 months post procedure. Results: The CDR demonstrated that those with lower pain levels, or high pain levels with previous surgery, could be predicted to benefit from a single IA BMAC injection. Conclusion: A simple CDR containing three variables predicted responsiveness to a single IA knee BMAC injection with high accuracy. Further validation of the CDR is required prior to routine use in clinical practice.

目的:建立一个简单的临床决策规则(CDR),以确定谁可能或不可能受益于骨髓浓缩物(BMAC)注射的膝骨关节炎患者。材料与方法:共有92例具有临床和影像学证据的难治性膝骨性关节炎患者接受单次关节内(IA) BMAC注射。采用多元logistic回归分析确定哪些危险因素组合可预测BMAC反应性。应答者定义为术后6个月膝关节疼痛较基线改善15%以上的患者。结果:CDR显示,疼痛程度较低或既往手术疼痛程度较高的患者可以从单次IA BMAC注射中获益。结论:一个包含三个变量的简单CDR预测对单次IA膝关节BMAC注射的反应性具有很高的准确性。在临床常规使用之前,需要对CDR进行进一步的验证。
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引用次数: 0
Efficacy of platelet-rich plasma for sacroiliac joint dysfunction: a qualitative systematic review with pooled analysis. 富血小板血浆治疗骶髂关节功能障碍的疗效:一项综合分析的定性系统评价。
IF 2.7 4区 医学 Q1 Medicine Pub Date : 2023-06-01 DOI: 10.2217/rme-2022-0211
Brandon Goodwin, Nicholas Averell, Usmaan Al-Shehab, Akhmad Ernazarov, Lisa Price, Akhil Choudhary, Richard Jermyn

Aim: We aimed to expound upon previous research examining the effect of platelet rich plasma in the treatment of sacroiliac joint (SIJ) dysfunction and pain. Materials & methods: A systematic review was employed in conjunction with a pooled analysis of the efficacy of platelet-rich plasma (PRP) in SIJ dysfunction and pain. Results: A total of 259 articles were retrieved following database systematic review. As a result, four clinical trials and two case studies were subjected to full text appraisal. The dates of publication ranged from 2015 to 2022. Conclusion: Although a unique modality, there is not enough evidence to support the employment of PRP injections over current steroid standard of care. Further double-blinded, randomized control trials are required to elucidate PRP role in SIJ dysfunction.

目的:对富血小板血浆治疗骶髂关节(SIJ)功能障碍和疼痛的疗效进行综述。材料与方法:系统回顾并汇总分析富血小板血浆(PRP)治疗SIJ功能障碍和疼痛的疗效。结果:经数据库系统审查,共检索到259篇文献。因此,对四项临床试验和两项案例研究进行了全文评估。出版日期从2015年到2022年不等。结论:虽然是一种独特的治疗方式,但没有足够的证据支持PRP注射优于目前的类固醇治疗标准。需要进一步的双盲、随机对照试验来阐明PRP在SIJ功能障碍中的作用。
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引用次数: 1
Critical elements in tissue engineering of craniofacial malformations. 颅面畸形组织工程的关键因素。
IF 2.7 4区 医学 Q1 Medicine Pub Date : 2023-06-01 DOI: 10.2217/rme-2022-0128
Ali Gholamalizadeh, Sajed Nazifkerdar, Nasim Safdarian, Aida Eyvaz Ziaee, Hamid Mobedi, Reza Rahbarghazi, Mehdi Khanmohammadi, Sepideh Saghati

Abnormal craniofacial bone fusion can lead to the generation of several congenital malformations such as cleft palate, craniosynostosis and craniofacial skeletal hypoplasia, which physically and mentally affect patients. Conventional approaches for the treatment of craniofacial malformations such as the transplantation of autologous bone grafts are not completely efficient and usually, patients suffer from various complications. In line with these statements, the advent of novel therapeutic approaches in human medicine is mandatory. Regarding the extent, size and severity of the bone malformation, supplementation and release of oxygen molecules into the affected sites are critical issues for successful osteogenesis. Here, tissue engineering modalities associated with oxygen supplementation and novel approaches associated with hydrogel synthesis were highlighted in terms of craniofacial malformations.

颅面骨融合异常可导致腭裂、颅缝闭锁、颅面骨发育不全等几种先天性畸形的产生,对患者身心造成影响。传统的颅面畸形治疗方法如自体骨移植并不完全有效,而且患者通常会出现各种并发症。与这些声明一致,人类医学中出现新的治疗方法是强制性的。考虑到骨畸形的程度、大小和严重程度,氧分子的补充和释放是成功成骨的关键问题。在颅面畸形方面,强调了与氧气补充相关的组织工程模式和与水凝胶合成相关的新方法。
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引用次数: 0
Association of mesenchymal stem cells derived from bone marrow and adipose tissue enhances bone repair in rat calvarial defects. 骨髓间充质干细胞与脂肪组织的结合促进了大鼠颅骨缺损的骨修复。
IF 2.7 4区 医学 Q1 Medicine Pub Date : 2023-05-01 DOI: 10.2217/rme-2022-0219
Gabriela Guaraldo Campos Totoli, Rayana Longo Bighetti-Trevisan, Gileade Pereira Freitas, Leticia Faustino Adolpho, Adriana Luisa Golçalves Almeida, Ana Carolina Loyola Barbosa, Jaqueline Isadora Reis Ramos, Marcio Mateus Beloti, Adalberto Luiz Rosa

Aim: We evaluated the bone repair induced by MSCs from adipose tissue (AT-MSCs) and bone marrow (BM-MSCs) injected into rat calvarial defects at two time points. Methods & results: Both cell populations expressed MSC surface markers and differentiated into adipocytes and osteoblasts. μCT showed that the combination of cells from distinct sources exhibited synergistic effects to increase bone repair with an advantage when BM-MSCs were injected prior to AT-MSCs. The higher osteogenic potential of these MSC combinations was demonstrated using an in vitro coculture system where BM-MSCs and AT-MSCs association induced higher ALP activity in MC3T3-E1 cells. Conclusion: Our findings may drive new approaches to treat bone defects and shed light on the complexity of the mechanisms involved in bone regeneration.

目的:观察脂肪组织间充质干细胞(at -MSCs)和骨髓间充质干细胞(BM-MSCs)在两个时间点对大鼠颅骨缺损的骨修复作用。方法与结果:两组细胞均表达MSC表面标记,并分化为脂肪细胞和成骨细胞。μCT结果显示,不同来源的细胞组合在促进骨修复方面表现出协同效应,且在AT-MSCs之前注射BM-MSCs具有优势。在体外共培养系统中,BM-MSCs和AT-MSCs联合诱导MC3T3-E1细胞中更高的ALP活性,证明了这些MSC组合具有更高的成骨潜能。结论:我们的发现可能会推动治疗骨缺损的新方法,并揭示骨再生机制的复杂性。
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引用次数: 2
Lateral epicondylitis: comparing ultrasound-guided tenotomy with or without amniotic membrane allograft injection. 外侧上髁炎:超声引导下的肌腱切开术与不注射同种异体羊膜的比较。
IF 2.7 4区 医学 Q1 Medicine Pub Date : 2023-05-01 DOI: 10.2217/rme-2023-0027
Hirotaka Nakagawa, Kyungje Sung, Ken Mautner, Tabitha May, Walter I Sussman

Aim: To evaluate the clinical response to augmenting ultrasound-guided tenotomy (USGT) with an amniotic membrane (AM) allograft injection. Design: Retrospective study. Materials & methods: Subjects underwent either a USGT (N = 16) or a combined USGT plus AM injection (N = 14). Results: Both groups demonstrated a significant reduction in pain from baseline starting after 2 weeks in the USGT plus AM group (p = 0.036) and after 8 weeks in the USGT group (p = 0.021). The reduction in pain was sustained for the entire duration of the study (52 weeks). There was no significant difference in pain levels at 26- or 52-week follow-up or patient satisfaction between the two groups. Conclusion: USGT with or without AM allograft injections results in a significant reduction in pain with high patient satisfaction.

目的:评价超声引导下羊膜(AM)增强肌腱切开术(USGT)的临床疗效。设计:回顾性研究。材料与方法:受试者接受了USGT (N = 16)或USGT加AM注射(N = 14)。结果:两组疼痛均在USGT + AM组2周后(p = 0.036)和USGT组8周后(p = 0.021)从基线开始显著减轻。疼痛的减轻持续了整个研究期间(52周)。在26周或52周的随访中,两组患者的疼痛程度和患者满意度没有显著差异。结论:采用同种异体AM移植注射或不采用同种异体AM移植注射可显著减轻疼痛,患者满意度高。
{"title":"Lateral epicondylitis: comparing ultrasound-guided tenotomy with or without amniotic membrane allograft injection.","authors":"Hirotaka Nakagawa,&nbsp;Kyungje Sung,&nbsp;Ken Mautner,&nbsp;Tabitha May,&nbsp;Walter I Sussman","doi":"10.2217/rme-2023-0027","DOIUrl":"https://doi.org/10.2217/rme-2023-0027","url":null,"abstract":"<p><p><b>Aim:</b> To evaluate the clinical response to augmenting ultrasound-guided tenotomy (USGT) with an amniotic membrane (AM) allograft injection. <b>Design:</b> Retrospective study. <b>Materials & methods:</b> Subjects underwent either a USGT (N = 16) or a combined USGT plus AM injection (N = 14). <b>Results:</b> Both groups demonstrated a significant reduction in pain from baseline starting after 2 weeks in the USGT plus AM group (p = 0.036) and after 8 weeks in the USGT group (p = 0.021). The reduction in pain was sustained for the entire duration of the study (52 weeks). There was no significant difference in pain levels at 26- or 52-week follow-up or patient satisfaction between the two groups. <b>Conclusion:</b> USGT with or without AM allograft injections results in a significant reduction in pain with high patient satisfaction.</p>","PeriodicalId":21043,"journal":{"name":"Regenerative medicine","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9595728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
Regenerative medicine
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