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Early delivery of human umbilical cord mesenchymal stem cells improves healing in a rat model of Achilles tendinopathy. 早期输送人脐带间充质干细胞可改善跟腱病大鼠模型的愈合。
IF 2.4 4区 医学 Q4 CELL & TISSUE ENGINEERING Pub Date : 2024-02-01 Epub Date: 2024-02-28 DOI: 10.2217/rme-2023-0222
Ze Yuan, Ding Yu, Yanxue Wang, Lijiaqi Liu, Junchao Wang, Chao Ma, Shaoling Wu

Objective: This study aimed to explore the efficacy and optimal delivery time of human umbilical cord mesenchymal stem cells (hUC-MSCs) in treating collagenase-induced Achilles tendinopathy. Methods: Achilles tendinopathy in rats at early or advanced stages was induced by injecting collagenase I into bilateral Achilles tendons. A total of 28 injured rats were injected with a hUC-MSC solution or normal saline into bilateral tendons twice and sampled after 4 weeks for histological staining, gene expression analysis, transmission electron microscope assay and biomechanical testing analysis. Results: The results revealed better histological performance and a larger collagen fiber diameter in the MSC group. mRNA expression of TNF-α, IL-1β and MMP-3 was lower after MSC transplantation. Early MSC delivery promoted collagen I and TIMP-3 synthesis, and strengthened tendon toughness. Conclusion: hUC-MSCs demonstrated a therapeutic effect in treating collagenase-induced Achilles tendinopathy, particularly in the early stage of tendinopathy.

研究目的本研究旨在探讨人脐带间充质干细胞(hUC-MSCs)治疗胶原酶诱导的跟腱病的疗效和最佳给药时间。研究方法通过向双侧跟腱注射胶原酶I,诱导早期或晚期大鼠跟腱病变。对 28 只受伤大鼠的双侧肌腱注射两次 hUC 间充质干细胞溶液或生理盐水,4 周后取样进行组织学染色、基因表达分析、透射电子显微镜检测和生物力学测试分析。结果显示结果显示,间充质干细胞组的组织学表现更好,胶原纤维直径更大;间充质干细胞移植后 TNF-α、IL-1β 和 MMP-3 的 mRNA 表达量更低。早期间充质干细胞的输送促进了胶原蛋白I和TIMP-3的合成,增强了肌腱的韧性。结论:hUC-间充质干细胞对治疗胶原酶诱导的跟腱病具有疗效,尤其是在跟腱病的早期阶段。
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引用次数: 0
The Glossary for Advanced Therapies. 高级疗法词汇》。
IF 2.7 4区 医学 Q1 Medicine Pub Date : 2024-01-16 DOI: 10.2217/rme-2023-1812s
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引用次数: 0
Industry updates from the field of stem cell research and regenerative medicine in March 2024. 2024 年 3 月干细胞研究和再生医学领域的行业动态。
IF 2.4 4区 医学 Q4 CELL & TISSUE ENGINEERING Pub Date : 2024-01-01 Epub Date: 2024-05-28 DOI: 10.1080/17460751.2024.2353496
Dusko Ilic, Mirjana Liovic

Latest developments in the field of stem cell research and regenerative medicine compiled from publicly available information and press releases from non-academic institutions in March 2024.

2024 年 3 月干细胞研究和再生医学领域的最新进展,汇编自公开信息和非学术机构的新闻稿。
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引用次数: 0
Industry updates from the field of stem cell research and regenerative medicine in April 2024. 2024 年 4 月干细胞研究和再生医学领域的行业动态。
IF 2.4 4区 医学 Q4 CELL & TISSUE ENGINEERING Pub Date : 2024-01-01 Epub Date: 2024-06-28 DOI: 10.1080/17460751.2024.2365027
Dusko Ilic, Mirjana Liovic

Latest developments in the field of stem cell research and regenerative medicine compiled from publicly available information and press releases from non-academic institutions in April 2024.

2024 年 4 月干细胞研究和再生医学领域的最新进展,汇编自公开信息和非学术机构的新闻稿。
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引用次数: 0
CGRP promotes osteogenic differentiation by regulating macrophage M2 polarization through HDAC6/AKAP12 signaling pathway. CGRP 通过 HDAC6/AKAP12 信号通路调节巨噬细胞 M2 极化,从而促进成骨分化。
IF 2.4 4区 医学 Q4 CELL & TISSUE ENGINEERING Pub Date : 2024-01-01 Epub Date: 2024-07-29 DOI: 10.1080/17460751.2024.2370697
Wenjing Chen, Lin Ma, Wencai Sun, Wenlong Xiao, Hao Guo, Jiang Xiu, Xin Jiang

Aim: To determine the mechanism of Calcitonin gene-related peptide (CGRP) in bone healing.Materials & methods: Alkaline phosphatase (ALP) activity and inflammatory-factor levels were detected using ELISA. Osteogenic differentiation was assessed using Alizarin red staining technique. The interaction between histone deacetylase 6 (HDAC6) and A-kinase anchoring protein 12 (AKAP12) was investigated through Co- immunoprecipitation.Results: CGRP treatment promoted rat bone marrow-derived macrophages (BMDMs) M2 polarization. CGRP facilitated osteogenic differentiation by enhancing M2 polarization of BMDMs. Mechanistically, CGRP promoted AKAP12 acetylation to activate the extracellular regulated protein kinases pathway by HDAC6 inhibition.Conclusion: CGRP promoted M2 polarization of rat BMDMs and facilitated osteogenic differentiation through the HDAC6/AKAP12/extracellular regulated protein kinases signaling pathway, thereby promoting bone healing.

目的:确定降钙素基因相关肽(CGRP)在骨愈合中的作用机制。材料与方法:用酶联免疫吸附法检测碱性磷酸酶(ALP)活性和炎症因子水平。使用茜素红染色技术评估成骨分化。通过共免疫沉淀法研究组蛋白去乙酰化酶6(HDAC6)和A-激酶锚定蛋白12(AKAP12)之间的相互作用。结果CGRP 可促进大鼠骨髓源性巨噬细胞(BMDMs)的 M2 极化。CGRP 通过增强 BMDMs 的 M2 极化促进成骨分化。从机制上讲,CGRP通过抑制HDAC6促进AKAP12乙酰化以激活细胞外调节蛋白激酶通路。结论CGRP能促进大鼠BMDMs的M2极化,并通过HDAC6/AKAP12/细胞外调节蛋白激酶信号通路促进成骨分化,从而促进骨愈合。
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引用次数: 0
Forging the way: pioneering regeneration in volume 19 of Regenerative Medicine. 锻造的方式:开拓再生医学19卷再生。
IF 2.4 4区 医学 Q4 CELL & TISSUE ENGINEERING Pub Date : 2024-01-01 Epub Date: 2023-11-29 DOI: 10.2217/rme-2023-0213
Jasmine Hagan, Megan Giboney

We are delighted to welcome you to the 19th volume of Regenerative Medicine. In this foreword, we reflect on the content highlights from 2023 and discuss what we can look forward to in the year ahead.

我们很高兴欢迎您来到《再生医学》第19卷。在这篇前言中,我们回顾了2023年的内容亮点,并讨论了我们对未来一年的期待。
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引用次数: 0
Patient, parent and professional expert perspectives on personalized regenerative implants: a qualitative focus group study. 患者、家长和专业专家对个性化再生植入物的看法:焦点小组定性研究。
IF 2.4 4区 医学 Q4 CELL & TISSUE ENGINEERING Pub Date : 2024-01-01 Epub Date: 2024-09-02 DOI: 10.1080/17460751.2024.2386214
Manon van Daal, Anne-Floor J de Kanter, Roel Jh Custers, Elena Martínez-Sanz, Annelien L Bredenoord, Nienke de Graeff

Background: Perspectives of patients, parents and professional experts on personalized regenerative implants for regenerative medicine purposes are largely unknown.Method: To better understand these perspectives, we conducted four focus groups with professional experts of mixed European nationality (n = 8), Dutch patients with regular implants (n = 8), Dutch and Belgian (n = 5) and Spanish (n = 8) parents of children with cleft palate.Results: Two overarching themes were identified: 'patient-centered research and care' and 'ambivalent attitudes toward personalized regenerative implants'.Discussion: The results reveal that stakeholders should adopt a participatory rather than an impairment discourse and address the ambivalence among professional experts, patients and parents.Conclusion: Considering stakeholder perspectives facilitates ethical and responsible development and use of personalized regenerative implants.

背景:患者、家长和专业专家对用于再生医学目的的个性化再生植入物的看法大多不为人知:为了更好地了解这些观点,我们与欧洲混合国籍的专业专家(n = 8)、接受常规植入物的荷兰患者(n = 8)、荷兰和比利时(n = 5)以及西班牙(n = 8)腭裂患儿的父母进行了四次焦点小组讨论:结果:确定了两大主题:结果:确定了两个总体主题:"以患者为中心的研究和护理 "和 "对个性化再生植入体的矛盾态度":讨论:研究结果表明,利益相关者应采取参与性而非损害性的讨论方式,并解决专业专家、患者和家长的矛盾态度:结论:考虑利益相关者的观点有助于开发和使用个性化再生植入体,并使其符合道德规范和责任要求。
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引用次数: 0
Safety of bone marrow derived mesenchymal stem cell extracellular vesicle injection for lumbar facet joint pain. 骨髓间充质干细胞细胞外囊注射治疗腰椎面关节疼痛的安全性。
IF 2.4 4区 医学 Q4 CELL & TISSUE ENGINEERING Pub Date : 2024-01-01 Epub Date: 2024-02-08 DOI: 10.2217/rme-2023-0110
James E Wilson, Bobbie A Today, Maria Salazar, Jonathann Kuo, John T Ransom, Amy L Lightner, Grace Chen, Anita Wong

Aim: A 3-month pilot study to evaluate the safety of injecting a bone marrow-derived mesenchymal stem cell extracellular vesicle advanced investigational product (IP) into the lumbar facet joint space as a treatment for chronic low back pain. Methods: 20 healthy adults were treated with IP injections (0.5 ml/joint) and evaluated by three functional assessments 1, 3, 7, 14, 30, 60 and 90 days later. Results: No adverse effects or complications occurred across the 3-month follow-up. There were no reports of worsening pain. After 3 months group average scores improved significantly (p < 0.0001) in the Severity Index (65.04%), Interference Index (72.09%) and Oswestry Disability Index (58.43%) assessments. Conclusion: IP injections were safe and associated with significant functional improvements.

目的:一项为期3个月的试验性研究,评估在腰椎面关节间隙注射骨髓间充质干细胞胞外囊高级研究产品(IP)治疗慢性腰痛的安全性。方法:对20名健康成年人进行IP注射(0.5毫升/关节)治疗,并在1、3、7、14、30、60和90天后进行三次功能评估。结果:3 个月的随访期间未出现不良反应或并发症。没有疼痛恶化的报告。3 个月后,治疗组的平均得分明显提高(p 结论:IP 注射是安全的,并与疼痛相关:IP 注射是安全的,并能显著改善功能。
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引用次数: 0
Reduction of Activin A gives rise to comparable expression of key definitive endoderm and mature beta cell markers. 减少 Activin A 会导致关键的最终内胚层和成熟β细胞标志物的表达不相上下。
IF 2.4 4区 医学 Q4 CELL & TISSUE ENGINEERING Pub Date : 2024-01-01 Epub Date: 2024-01-19 DOI: 10.2217/rme-2023-0187
Aldyn Wildey, Stephen Harrington, Lisa Stehno-Bittel, Francis Karanu

Aim: Cell therapies for diabetes rely on differentiation of stem cells into insulin-producing cells, which is complex and expensive. Our goal was to evaluate production costs and test ways to reduce it. Methods: Cost of Goods (COGs) analysis for differentiation was completed and the effects of replacement or reduction of the most expensive item was tested using qRT-PCR, immunohistochemistry, flow cytometry along with glucose-stimulated insulin release. Results: Activin A (AA) was responsible for significant cost. Replacement with small molecules failed to form definitive endoderm (DE). Reducing AA by 50% did not negatively affect expression of beta cell markers. Conclusion: Reduction of AA concentration is feasible without adversely affecting DE and islet-like cell differentiation, leading to significant cost savings in manufacturing.

目的:治疗糖尿病的细胞疗法依赖于将干细胞分化为胰岛素分泌细胞,这既复杂又昂贵。我们的目标是评估生产成本,并测试降低成本的方法。方法:使用 qRT-PCR、免疫组织化学、流式细胞术和葡萄糖刺激胰岛素释放法测试替代或减少最昂贵项目的效果。结果显示活化素 A(AA)的成本很高。用小分子替代无法形成确定性内胚层(DE)。将 AA 减少 50%不会对β细胞标记物的表达产生负面影响。结论:降低 AA 浓度是可行的,不会对 DE 和类岛细胞分化产生不利影响,从而大大节约了生产成本。
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引用次数: 0
Unconventional strategies for liver tissue engineering: plant, paper, silk and nanomaterial-based scaffolds. 肝脏组织工程的非常规策略:基于植物、纸张、丝绸和纳米材料的支架。
IF 2.4 4区 医学 Q4 CELL & TISSUE ENGINEERING Pub Date : 2024-01-01 Epub Date: 2024-08-05 DOI: 10.1080/17460751.2024.2378615
Sanyam Jain, Jai Gopal Sharma

The paper highlights how significant characteristics of liver can be modeled in tissue-engineered constructs using unconventional scaffolds. Hepatic lobular organization and metabolic zonation can be mimicked with decellularized plant structures with vasculature resembling a native-hepatic lobule vascular arrangement or silk blend scaffolds meticulously designed for guided cellular arrangement as hepatic patches or metabolic activities. The functionality of hepatocytes can be enhanced and maintained for long periods in naturally fibrous structures paving way for bioartificial liver development. The phase I enzymatic activity in hepatic models can be raised exploiting the microfibrillar structure of paper to allow cellular stacking creating hypoxic conditions to induce in vivo-like xenobiotic metabolism. Lastly, the paper introduces amalgamation of carbon-based nanomaterials into existing scaffolds in liver tissue engineering.

论文重点介绍了如何利用非常规支架在组织工程构建物中模拟肝脏的重要特征。肝小叶组织和新陈代谢分区可通过脱细胞植物结构进行模拟,该结构的脉管与原生肝小叶血管排列相似,或通过丝绸混合支架进行精心设计,以引导细胞排列为肝补片或新陈代谢活动。肝细胞的功能可在天然纤维结构中得到增强和长期保持,为生物人工肝的开发铺平了道路。利用纸张的微纤维结构,可以提高肝脏模型的 I 期酶活性,允许细胞堆叠,创造缺氧条件,诱导类似活体的异生物代谢。最后,论文介绍了在肝组织工程中将碳基纳米材料与现有支架的结合。
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引用次数: 0
期刊
Regenerative medicine
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