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Evaluation of bi-layer silk fibroin grafts for onlay urethroplasty in a rabbit model of urethral stricture disease. 在兔尿道狭窄疾病模型中评估双层丝纤维蛋白移植物用于尿道嵌顿成形术的效果。
IF 2.4 4区 医学 Q4 CELL & TISSUE ENGINEERING Pub Date : 2024-08-30 DOI: 10.1080/17460751.2024.2389753
Gokhan Gundogdu, Jay Budrewicz, Jodie Giordano, Raffaele Melidone, Chris Searcy, Vikas Agarwal, Carlos R Estrada, Joshua R Mauney

Background: Autologous tissues such as buccal mucosa (BM) are widely used for reconstruction of urethral strictures; however, limitations such as donor site morbidity and scarce tissue supply require the development of alternative biomaterials for urethral repair. The goals of this study were to determine the safety and efficacy of bi-layer silk fibroin (BLSF) matrices for urethral stricture repair and compare histological and functional outcomes to the standard approach, BM urethroplasty under good laboratory practices.Material and methods: A total of 13 rabbits exhibiting urethral stricture formation following electrocoagulation injury were treated with onlay urethroplasty with either acellular BLSF (N = 7) or autologous BM (N = 6) grafts for 3 months. Uninjured control rabbits were maintained in parallel (N = 4).Results and conclusion: Animals receiving BLSF implants were demonstrated to be functionally equivalent to BM grafts in their ability to restored strictured calibers, support micturition and promote tissue regeneration with minimal inflammation.

背景:颊粘膜(BM)等自体组织被广泛用于尿道狭窄的重建;然而,由于供体部位发病率和组织供应稀缺等限制,需要开发用于尿道修复的替代生物材料。本研究的目的是确定双层蚕丝纤维蛋白(BLSF)基质用于尿道狭窄修复的安全性和有效性,并将组织学和功能结果与标准方法--良好实验室规范下的 BM 尿道成形术--进行比较:共对 13 只电凝损伤后尿道狭窄的兔子进行了为期 3 个月的无细胞 BLSF(7 只)或自体 BM(6 只)移植物尿道成形术。同时饲养未受伤的对照组兔子(4 只):结果和结论:接受 BLSF 植入物的动物在功能上与 BM 移植物相当,它们能够恢复狭窄的管径、支持排尿并促进组织再生,同时炎症极少。
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引用次数: 0
Industry updates from the field of stem cell research and regenerative medicine in May 2024. 2024 年 5 月干细胞研究和再生医学领域的行业动态。
IF 2.4 4区 医学 Q4 CELL & TISSUE ENGINEERING Pub Date : 2024-08-05 DOI: 10.1080/17460751.2024.2378634
Dusko Ilic, Mirjana Liovic

Latest developments in the field of stem cell research and regenerative medicine compiled from publicly available information and press releases from non-academic institutions in May 2024.

2024 年 5 月干细胞研究和再生医学领域的最新进展,汇编自公开信息和非学术机构的新闻稿。
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引用次数: 0
A case series utilizing bone marrow aspirate concentrate, cancellous bone autograft, platelet-rich plasma and autologous fibrin for the treatment of femur nonunions 利用骨髓抽吸物浓缩物、松质骨自体移植、富血小板血浆和自体纤维蛋白治疗股骨非畸形的病例系列研究
IF 2.7 4区 医学 Q1 Medicine Pub Date : 2024-06-10 DOI: 10.1080/17460751.2024.2352250
O. Dulić, D. Abazovic, Gordan Gavrilovic, P. Rasovic, Nataša Janjić, M. Obradović, S. Ninkovic, Milan Tošić, Ivica Lalić, Branko Baljak, Milan Milinkov, Dusan Maric
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引用次数: 0
A value hierarchy for inclusive design of heart valve implants in regenerative medicine 再生医学中心脏瓣膜植入物包容性设计的价值层次结构
IF 2.7 4区 医学 Q1 Medicine Pub Date : 2024-06-10 DOI: 10.1080/17460751.2024.2357500
A. D. De Kanter, Manon Van Daal, Callum J. Gunn, A. Bredenoord, N. D. Graeff, K. Jongsma
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引用次数: 0
A sustainable approach to derive sheep corneal scaffolds from stored slaughterhouse waste 从屠宰场废弃物中提取羊角膜支架的可持续方法
IF 2.7 4区 医学 Q1 Medicine Pub Date : 2024-06-10 DOI: 10.1080/17460751.2024.2357499
Zehara M Ali, Xinyu Wang, Meklit G Shibru, Maha Alhosani, Nouf Alfadhli, Aysha Alnuaimi, Fiza F Murtaza, Aisha Zaid, Rodaina Khaled, Ahmed E. Salih, Hema Vurivi, Sayel Daoud, Haider Butt, Vincent Chan, Igor V Pantic, Jovana Paunovic, Peter R. Corridon
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引用次数: 0
The potential of induced pluripotent stem cell-derived retinal organoids in regenerative medicine 诱导多能干细胞衍生视网膜器官组织在再生医学中的潜力
IF 2.7 4区 医学 Q1 Medicine Pub Date : 2024-06-04 DOI: 10.1080/17460751.2024.2352257
Y. Hirami, M. Mandai
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引用次数: 0
Advances in regenerative rehabilitation in the rehabilitation of musculoskeletal injuries. 再生康复在肌肉骨骼损伤康复方面的进展。
IF 2.4 4区 医学 Q4 CELL & TISSUE ENGINEERING Pub Date : 2024-06-02 Epub Date: 2024-06-10 DOI: 10.1080/17460751.2024.2357956
Zirui Zhang, Pengfei Yao, Shuai Fan

In the rapidly advancing field of regenerative medicine, relying solely on cell transplantation alone may be insufficient for achieving functional recovery, and rehabilitation before and after transplantation is crucial. Regenerative rehabilitation functions by synergizing the therapeutic effects of regeneration and rehabilitation to maximize tissue regeneration and patient function. We used the keywords "regenerative rehabilitation" to search across the database for published works; this review discusses the development of regenerative rehabilitation for the treatment of musculoskeletal injuries. Rehabilitation has become a crucial component of regenerative medicine because it can enhance patients' functional activity and facilitate their early return to society. Experimental data increasingly demonstrates that rehabilitation interventions support the regeneration of transplanted tissues.

在快速发展的再生医学领域,仅仅依靠细胞移植可能不足以实现功能恢复,移植前后的康复治疗至关重要。再生康复的作用是将再生和康复的治疗效果协同起来,最大限度地提高组织再生和患者功能。我们使用关键词 "再生康复 "在数据库中搜索已发表的作品;本综述讨论了再生康复在治疗肌肉骨骼损伤方面的发展。康复已成为再生医学的重要组成部分,因为它能增强患者的功能活动,促进他们早日重返社会。越来越多的实验数据表明,康复干预有助于移植组织的再生。
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引用次数: 0
Facilitating the ethical sourcing of donor hematopoietic stem cells for cell and gene therapy research and development. 为细胞和基因疗法的研究与开发提供符合道德标准的造血干细胞捐献来源。
IF 2.4 4区 医学 Q4 CELL & TISSUE ENGINEERING Pub Date : 2024-06-02 Epub Date: 2024-06-18 DOI: 10.1080/17460751.2024.2357930
Lina Hamad, Choon Key Chekar, Chloe Anthias, Laura Machin

Aim: Unrelated stem cell donor registries (DRs) are increasingly engaging in the field of cell and gene therapy (CGT). This study aims to explore the values, concerns, needs and expectations of donors and members of the public on donating hematopoietic stem cells (HSCs) for CGT.Methods: Seven focus groups were conducted in 2019 with members of the public, prospective donors and donors on the Anthony Nolan DR in the UK.Results: Participants expressed concerns over increased frequency of donation and incidental findings and required more information on the type of research including the purpose and possible outcomes.Conclusion: Addressing donors' concerns, needs and expectations on donating cellular materials for CGT research and development is essential to maintaining the highest standards for donor care and safety within this rapidly emerging field.

目的:非亲缘干细胞捐献者登记处(DRs)越来越多地参与到细胞和基因治疗(CGT)领域。本研究旨在探讨捐献者和公众对捐献造血干细胞用于细胞和基因治疗的价值观、关注点、需求和期望。研究方法于2019年与公众、潜在捐献者和英国安东尼诺兰DR的捐献者开展了七次焦点小组讨论。结果参与者对捐赠频率增加和偶然发现表示担忧,并要求获得更多有关研究类型的信息,包括目的和可能的结果。结论:解决捐献者对捐献细胞材料用于 CGT 研究和开发的担忧、需求和期望,对于在这一迅速崛起的领域保持最高标准的捐献者护理和安全至关重要。
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引用次数: 0
PXL01 alters macrophage response with no effect on axonal outgrowth or Schwann cell response after nerve repair in rats. PXL01 可改变巨噬细胞的反应,但对大鼠神经修复后的轴突生长或许旺细胞反应没有影响。
IF 2.4 4区 医学 Q4 CELL & TISSUE ENGINEERING Pub Date : 2024-06-02 Epub Date: 2024-07-03 DOI: 10.1080/17460751.2024.2361515
Derya Burcu Hazer Rosberg, Lena Stenberg, Margit Mahlapuu, Lars B Dahlin

Background: Adjunctive pharmacological treatment may improve nerve regeneration. We investigated nerve regeneration processes of PXL01 - a lactoferrin-derived peptide - after repair of the sciatic nerve in healthy Wistar rats.Materials & methods: PXL01, sodium hyaluronate (carrier) or sodium chloride was administered around the repair. After 6 days axonal outgrowth, Schwann cell response, pan- (CD68) and pro-healing (CD206) macrophages in sciatic nerve, sensory neuronal response in dorsal root ganglia (DRG) and expression of heat shock protein 27 (HSP27) in sciatic nerves and DRGs were analyzed.Results: Despite a lower number of pan-macrophages, other investigated variables in sciatic nerves or DRGs did not differ between the treatment groups.Conclusion: PLX01 applied locally inhibits inflammation through pan-macrophages in repaired sciatic nerves without any impact on nerve regeneration or pro-healing macrophages.

背景:辅助药物治疗可改善神经再生。我们研究了乳铁蛋白衍生肽 PXL01 在健康 Wistar 大鼠坐骨神经修复后的神经再生过程。材料与方法:在修复处周围注射 PXL01、透明质酸钠(载体)或氯化钠。6 天后,分析坐骨神经的轴突生长、许旺细胞反应、泛(CD68)和促愈合(CD206)巨噬细胞、背根神经节(DRG)的感觉神经元反应以及坐骨神经和 DRG 的热休克蛋白 27(HSP27)表达。结果:尽管泛巨噬细胞的数量较少,但坐骨神经或背根神经节的其他研究变量在治疗组之间没有差异。结论局部应用 PLX01 可通过修复的坐骨神经中的泛巨噬细胞抑制炎症,而不会影响神经再生或促进愈合的巨噬细胞。
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引用次数: 0
Industry updates from the field of stem cell research and regenerative medicine in February 2024. 2024 年 2 月干细胞研究和再生医学领域的行业动态。
IF 2.4 4区 医学 Q4 CELL & TISSUE ENGINEERING Pub Date : 2024-06-02 Epub Date: 2024-04-30 DOI: 10.1080/17460751.2024.2340888
Dusko Ilic, Mirjana Liovic

Latest developments in the field of stem cell research and regenerative medicine compiled from publicly available information and press releases from non-academic institutions in February 2024.

2024 年 2 月干细胞研究和再生医学领域的最新进展,汇编自公开信息和非学术机构的新闻稿。
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引用次数: 0
期刊
Regenerative medicine
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