Research in veterinary science最新文献_第8页

Development of a simplified model for assessing bovine leukemia virus transmission risk using enzyme-linked immunosorbent assay sample/positive ratios 利用酶联免疫吸附试验样品/阳性比率评估牛白血病病毒传播风险的简化模型的建立。

IF 1.8 3区农林科学 Q1 VETERINARY SCIENCES

Research in veterinary science

Pub Date : 2025-11-08 DOI: 10.1016/j.rvsc.2025.105972

Yuta Hamada , Tomoko Hiroe , Takao Oomoto , Erika Saito , Hiroho Ishida , Makoto Nagai , Hironobu Murakami

The bovine leukemia virus (BLV) is the primary cause of enzootic bovine leukosis in cattle, resulting in considerable economic impacts, including decreased milk production, increased disease susceptibility, and limitations on trade. While quantifying proviral load (PVL) through quantitative PCR (qPCR) effectively assesses BLV transmissibility, its high cost restricts widespread implementation. This study developed a simplified enzyme-linked immunosorbent (ELISA)-based method utilizing the sample-to-positive (S/P) ratio to estimate BLV transmission risk. Blood samples were obtained from 496 cattle (Japanese Black and Holstein-Friesian) in Japan. BLV infection was identified using ELISA and qPCR, with PVL measured as the number of BLV genome copies per 100,000 cells. The findings showed a positive correlation between the S/P ratio and PVL. Through receiver operating characteristic analysis, an S/P ratio cutoff of 1.87 was determined to differentiate ultra-low-risk cows (PVL < 600 copies/10⁵ cells) from those at elevated transmission risk, achieving 93.0 % sensitivity and 77.8 % specificity. This S/P ratio threshold accurately classified 94.3 % of ultra-low-risk animals and effectively identified cattle with a higher likelihood of horizontal transmission. The approach offers a cost-effective and scalable alternative to qPCR, particularly suitable for large-scale farms or areas with limited resources. Although less accurate than PVL-based techniques, S/P ratio-driven risk evaluation could significantly improve BLV management by facilitating the prompt detection and control of high-risk cattle.

牛白血病病毒（BLV）是牛流行性牛白血病的主要原因，造成相当大的经济影响，包括产奶量下降、疾病易感性增加和贸易限制。虽然通过定量PCR （qPCR）来定量前病毒载量（PVL）可以有效地评估BLV的传播性，但其高昂的成本限制了其广泛应用。本研究建立了一种基于简化酶联免疫吸附（ELISA）的方法，利用样本与阳性（S/P）比来估计BLV传播风险。从日本的496头牛（日本黑牛和荷尔斯泰因-弗里西亚牛）获得血液样本。使用ELISA和qPCR鉴定BLV感染，PVL以每100,000个细胞中BLV基因组拷贝数测量。结果显示，S/P比值与PVL呈正相关。通过受体工作特征分析，确定了区分超低风险奶牛（pvl5细胞）与高传播风险奶牛的S/P比临界值为1.87，灵敏度为93.0%，特异性为77.8%。这个S/P比率阈值准确地分类了94.3%的超低风险动物，并有效地识别了水平传播可能性较高的牛。该方法为qPCR提供了一种具有成本效益和可扩展的替代方法，特别适用于大规模养殖场或资源有限的地区。尽管与基于ppl的技术相比，S/P比率驱动的风险评估的准确性较低，但通过促进对高风险牛的及时发现和控制，可以显著改善BLV管理。

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引用次数: 0

Platelet lysate–silk fibroin injectable hydrogel as a potential biofunctional and biomechanical platform for canine intervertebral disc repair 血小板裂解-丝素蛋白注射水凝胶作为犬椎间盘修复的潜在生物功能和生物力学平台。

IF 1.8 3区农林科学 Q1 VETERINARY SCIENCES

Research in veterinary science

Pub Date : 2025-11-08 DOI: 10.1016/j.rvsc.2025.105971

Kannika Chayatup , Sarinthorn Boonkruephan , Saran Keeratihattayakorn , Jirasak Jitpibull , Chalika Wangdee , Juthamas Ratanavaraporn

Intervertebral disc degeneration (IVDD) in dogs remains a major clinical problem that requires regenerative strategies capable of restoring both biological activity and mechanical stability. This study developed and evaluated an injectable platelet lysate–silk fibroin (PL–TSF) hydrogel designed as a dual-functional platform for disc repair. Biofunctional and biomechanical performances were examined using canine chondrocyte culture and nucleotomized spinal segments. The PL–TSF hydrogel provided a controlled trophic microenvironment that enhanced chondrocyte proliferation and induced early upregulation of anabolic markers (SOX9, ACAN, COL2A1), while concurrently suppressing catabolic (MMP13) and inflammatory (IL1B) responses compared with controls. In contrast, direct platelet lysate exposure triggered an acute anabolic burst that was accompanied by elevated catabolic signaling. Biomechanically, the hydrogel enhanced viscoelastic damping and partially restored axial stiffness, indicating improved load-distribution capability after discectomy. These results demonstrate the dual biofunctional and biomechanical advantages of the PL–TSF hydrogel, providing controlled trophic stimulation and transient mechanical reinforcement that address both cellular regeneration and structural stabilization in intervertebral disc repair. This highlights its potential as a minimally invasive regenerative therapy for canine IVDD.

犬椎间盘退变（IVDD）仍然是一个主要的临床问题，需要能够恢复生物活性和机械稳定性的再生策略。本研究开发并评估了一种可注射血小板裂解-丝素蛋白（PL-TSF）水凝胶，该水凝胶被设计为椎间盘修复的双重功能平台。采用犬软骨细胞培养和核去核脊髓节段检测其生物功能和生物力学性能。与对照组相比，PL-TSF水凝胶提供了一个可控的营养微环境，增强了软骨细胞增殖，诱导了合成代谢标志物（SOX9、ACAN、COL2A1）的早期上调，同时抑制了分解代谢（MMP13）和炎症（IL1B）反应。相反，直接暴露于血小板裂解液会引发急性合成代谢爆发，并伴有分解代谢信号升高。生物力学方面，水凝胶增强了粘弹性阻尼，部分恢复了轴向刚度，表明椎间盘切除术后载荷分配能力得到改善。这些结果证明了PL-TSF水凝胶在生物功能和生物力学方面的双重优势，在椎间盘修复中提供了可控制的营养刺激和短暂的机械强化，解决了细胞再生和结构稳定问题。这突出了其作为犬IVDD微创再生治疗的潜力。

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引用次数: 0

Phylogeography of rabies virus transmitted by the hematophagous bat Desmodus rotundus in the state of São Paulo, Brazil 巴西<s:1>圣保罗州食血蝙蝠圆齿蝠传播狂犬病毒的系统地理学研究。

IF 1.8 3区农林科学 Q1 VETERINARY SCIENCES

Research in veterinary science

Pub Date : 2025-11-07 DOI: 10.1016/j.rvsc.2025.105950

M. Cocchi , C.M. Barbosa , J.G. Garcia , J. Santos , D.M. Junqueira , P. Carnieli JR , H.B.C.R. Batista

Rabies is a zoonosis primarily transmitted through bites from infected animals. The rabies virus (RABV) remains a significant public health concern in some countries and causes substantial economic losses in livestock farming. In Brazil, around 45,000 cattle die annually due to rabies, resulting in an estimated economic impact of up to 15 million dollars. Phylogeographic analysis is a valuable tool for tracking viral dispersion over time and space and is commonly used to monitor RABV's molecular epidemiology. This study aimed to characterize the dispersion of RABV isolated from cattle infected by hematophagous bats Desmodus rotundus in São Paulo state. Seventy-nine RABV samples collected between 2018 and 2020 were genetically characterized, focusing on the nucleoprotein gene, followed by sequencing and phylogenetic reconstruction. The analysis revealed four distinct lineages of RABV circulating among cattle in São Paulo. Phylogeographic inference traced the time to the most recent common ancestor (TMRCA) for two clades: SP Endemic (Clade 01) emerged around 1977, likely introduced from Minas Gerais, while Pan-American (Clade 03) dates to 1972, originating from viruses already circulating in São Paulo. Pan-American persisted for years and spread to Goiás, Tocantins, and Rio de Janeiro. These findings indicate that multiple RABV lineages co-circulate in São Paulo, with Campinas identified as a key initial hotspot for viral dissemination within the state.

狂犬病是一种人畜共患病，主要通过受感染动物的咬伤传播。狂犬病病毒（RABV）在一些国家仍然是一个重大的公共卫生问题，并在畜牧业中造成重大的经济损失。在巴西，每年大约有45000头牛死于狂犬病，造成的经济损失估计高达1500万美元。系统地理分析是追踪病毒随时间和空间扩散的有价值的工具，通常用于监测RABV的分子流行病学。本研究旨在对圣保罗州（o Paulo state）牛吸血蝙蝠（Desmodus rotundus）感染的RABV分离株的传播特性进行研究。对2018年至2020年收集的79份RABV样本进行遗传表征，重点研究核蛋白基因，然后进行测序和系统发育重建。分析显示，在圣保罗牛中传播的RABV有四种不同的谱系。系统地理推断将时间追溯到两个进化支最近的共同祖先（TMRCA）： SP地方病（进化支01）出现于1977年左右，可能是从米纳斯吉拉斯州传入的，而泛美（进化支03）出现于1972年，起源于已经在圣保罗传播的病毒。泛美持续了数年，并蔓延到Goiás、托坎廷斯和巴西里约热内卢。这些发现表明，多个RABV谱系在圣保罗州共同传播，坎皮纳斯被确定为病毒在该州传播的关键初始热点。

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引用次数: 0

Effect of polystyrene nanoplastics on in vitro maturation of pig cumulus-encosed oocytes 聚苯乙烯纳米塑料对猪卵母细胞体外成熟的影响。

IF 1.8 3区农林科学 Q1 VETERINARY SCIENCES

Research in veterinary science

Pub Date : 2025-11-07 DOI: 10.1016/j.rvsc.2025.105949

Marcella Spinaci , Sofia Dindo , Nadia Govoni , Laura Tovar , Alessandro Marino Volsa , Cinzia Cappannari , Diego Bucci , Jose Manuel Ortiz-Rodriguez

In recent years, concern has been increasing over the impact of environmental nanoplastics (NPs) contamination on both human and animal health, particularly regarding potential effects on reproductive systems. Nevertheless, current knowledge about the hazards posed by nanoplastics to mammalian gametes remains limited. In this study we evaluated the impact of increasing concentrations of polystyrene nanoplastics (PS-NPs)(5, 50, 100 and 200 μg/mL) on female gamete, using an in vitro model of pig oocyte maturation (IVM). Nuclear maturation, cytoplasmic maturation and developmental competence of oocytes, intracellular oocyte levels of glutathione (GSH) and reactive oxygen species (ROS), as well as steroidogenic activity of cumulus cells (CCs) were evaluated. Exposure to PS-NPs during IVM did not affect CCs steroidogenesis, oocyte nuclear maturation and oocyte cytoplasmic maturation in term of both fertilization parameters after IVF and blastocyst rate after parthenogenic activation. Nevertheless, PS-NPs significantly increased oocyte ROS levels at all the concentrations tested and compromised oocyte developmental competence, as indicated by reduced blastomere number per blastocyst. Our results confirm that PS-NPs may interfere with oocyte maturation and highlights the need to assess NPs exposure as an emerging environmental factor with potential implications for both animal and human fertility.

近年来，人们越来越关注环境纳米塑料污染对人类和动物健康的影响，特别是对生殖系统的潜在影响。然而，目前关于纳米塑料对哺乳动物配子的危害的知识仍然有限。本研究通过体外猪卵母细胞成熟（IVM）模型，研究了聚苯乙烯纳米塑料（PS-NPs）浓度（5、50、100和200 μg/mL）对雌性配子的影响。评估卵母细胞的核成熟、细胞质成熟和发育能力，卵母细胞内谷胱甘肽（GSH）和活性氧（ROS）水平，以及积云细胞（CCs）的甾体生成活性。体外受精期间暴露于PS-NPs对体外受精后的卵母细胞甾体形成、卵母细胞核成熟和卵母细胞细胞质成熟均没有影响，对孤雌激活后的囊胚率也没有影响。然而，PS-NPs在所有测试浓度下都显著增加了卵母细胞ROS水平，并损害了卵母细胞的发育能力，这表明每个囊胚的卵裂球数量减少。我们的研究结果证实，PS-NPs可能会干扰卵母细胞成熟，并强调有必要评估NPs暴露作为一种新兴的环境因素，对动物和人类的生育能力都有潜在影响。

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引用次数: 0

Comparison study on numbers of cells to count in differential counts of bronchoalveolar lavage fluid from calves 犊牛支气管肺泡灌洗液鉴别计数中待计数细胞数的比较研究

IF 1.8 3区农林科学 Q1 VETERINARY SCIENCES

Research in veterinary science

Pub Date : 2025-11-05 DOI: 10.1016/j.rvsc.2025.105952

Justine Clinquart, Thomas Lowie, Stan Jourquin, Bart Pardon

Small-volume, non-bronchoscopic bronchoalveolar lavage, which is currently used for pathogen identification in calves, holds potential for respiratory cytology. Cytology could assist in diagnosing respiratory tract infections and investigating airway inflammation. However, the time required to perform a differential count limits its practical feasibility. Therefore, counts based on 200 and 300 cells were compared to 500 cells in bronchoalveolar lavage fluid cytospin preparations of 134 calves showing at least one clinical sign of respiratory disease (29 herds, mostly Holstein Friesian and Belgian blue). Samples conveniently collected in previous studies were retrospectively examined. Passing-Bablok analyses and Bland-Altman plots with tolerance intervals were performed. Apart from a small constant bias (−0.21, 95 % confidence interval [CI] = −0.36 to −0.10) for lymphocytes in the 200 cell count (CC), Passing-Bablok analyses did not show a significant bias. For the 300 CC, tolerance intervals were ‐3.6 to 4.0 % for macrophages, −4.1 to 3.7 % for neutrophils, and −1.6 to 1.8 % for lymphocytes, all below the chosen maximum clinically accepted differences (5 % for macrophages and neutrophils, 2.5 % for lymphocytes). For the 200 CC, tolerance intervals slightly surpassed these (−6.0 to 5.7 % macrophages, −5.5 to 6.1 % neutrophils, −2.7 to 2.5 % lymphocytes). Most samples did not contain eosinophils, hindering conclusive analyses. The maximum observed difference was 1.7 % for the 200 CC. Although eosinophils still need investigation, the 300 CC proved a valuable alternative to the 500 CC. Furthermore, when the relatively higher deviations of the 200 CC are kept in mind, this could offer a very time-efficient alternative for routine diagnostics.

小容量，非支气管镜支气管肺泡灌洗，目前用于小牛病原体鉴定，具有呼吸细胞学的潜力。细胞学可以帮助诊断呼吸道感染和调查气道炎症。然而，执行差分计数所需的时间限制了其实际可行性。因此，对134头表现出至少一种呼吸道疾病临床症状的小牛（29头牛，主要为荷斯坦黑马和比利时蓝牛）的支气管肺泡灌洗液细胞自旋制剂中200和300个细胞的计数进行了比较。在以前的研究中方便收集的样本进行回顾性检查。进行了pass - bablok分析和Bland-Altman图。在200细胞计数（CC）中，淋巴细胞除了有一个小的恒定偏差（- 0.21,95%置信区间[CI] = - 0.36至- 0.10）外，Passing-Bablok分析没有显示出显著的偏差。对于300cc，巨噬细胞耐受区间为‐3.6 - 4.0%，中性粒细胞耐受区间为−4.1 - 3.7%，淋巴细胞耐受区间为−1.6 - 1.8%，均低于所选择的临床可接受的最大差异（巨噬细胞和中性粒细胞耐受区间为5%，淋巴细胞耐受区间为2.5%）。对于200cc，耐受间隔略高于这些（- 6.0 - 5.7%巨噬细胞，- 5.5 - 6.1%中性粒细胞，- 2.7 - 2.5%淋巴细胞）。大多数样品不含嗜酸性粒细胞，妨碍了结论性分析。200cc的最大观察差异为1.7% .尽管仍需对嗜酸性粒细胞进行调查，但300cc被证明是500cc的有价值的替代方案。此外，当200cc相对较高的偏差被记住时，这可以为常规诊断提供非常省时的替代方案。

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引用次数: 0

Effects of alternative bedding materials on metabolism, performance, hygiene, immune-related genes expression and meat quality of broilers under conventional and organic production systems 替代床上材料对传统和有机生产方式下肉鸡代谢、生产性能、卫生、免疫相关基因表达和肉品质的影响

IF 1.8 3区农林科学 Q1 VETERINARY SCIENCES

Research in veterinary science

Pub Date : 2025-11-05 DOI: 10.1016/j.rvsc.2025.105953

Nicolò Mezzasalma , Giorgia Mantovani , Marica Simoni , Arianna Goi , Rosario Pitino , Tommaso Danese , Lavrentia Karatosidi , Melania Andrani , Roberta Saleri , Clotilde Silvia Cabassi , Costanza Spadini , Luisa Ragionieri , Massimo De Marchi , Eleni Tsiplakou , Federico Righi

This study evaluated two alternative bedding materials (poplar pellet-PP and vine pellets-VP) in against conventional wood shavings (WS) on production performance, health status, hygiene, immune-related genes expression and meat quality of broilers reared in organic-like conditions. A total of 252 male Ross-308 chicks were assigned to 9 pens in a randomized blocked design with 3 replicates; 50 % were slaughtered at 42d while the remaining at 84d in accordance with organic farming regulations. Broilers raised on VP resulted in lower body and carcass weight than those on PP, which had the lowest feed conversion ratio at 63d. Compared to WS broilers on PP were heavier, cleaner and had lower water consumption, water consumption ratio, and water to feed ratio in organic rearing period. Pelleted beddings were drier until mid-trial; PP had a consistently higher fiber content (aNDF, ADF, lignin) than WS. Despite higher microbiological contamination pelleted beddings did not affect Lactobacillus spp. growth. Poplar pellet increased footpad and hock score (HS), while VP improved HS and plumage cleanliness (CS) at 28d but worsened HS at 84d. A blood protein reduction was observed in pellet beddings leading to increased creatinine levels. Birds reared on PP had the greatest thickness of tunica mucosa, villus-height (VH) and VH /crypt depth ratio at 42d. At 84d, VP increased IL-8 expression and reduced survival rate compared to PP. Overall, from a production perspective, pelleted beddings are suitable for fast-growing broiler hybrids under conventional and organic regimens. The PP is particularly recommended for organic production systems.

本研究评价了两种床上材料（杨木颗粒- pp和藤木颗粒- vp）替代常规木屑（WS）对肉鸡生产性能、健康状况、卫生状况、免疫相关基因表达和肉品质的影响。选取252只罗斯-308雄性雏鸡，采用随机区隔设计，每组设3个重复；50%在42天屠宰，其余在84天屠宰，按照有机农业规定。用VP饲养的肉鸡体重和胴体重低于用PP饲养的肉鸡，在63d时饲料系数最低。与WS相比，有机饲养期PP肉鸡更重、更清洁，耗水量、耗水比和水料比更低。颗粒状床层在试验中期之前较为干燥；PP的纤维含量（aNDF、ADF、木质素）始终高于WS。尽管有较高的微生物污染，但颗粒床层不影响乳酸菌的生长。杨树颗粒在28d时提高了雏鸟的足垫和飞节评分（HS），而VP在84d时提高了HS和羽毛清洁度（CS），使HS恶化。在颗粒床层中观察到血蛋白减少导致肌酐水平升高。PP饲养的雏鸟在42d时膜粘膜厚度、绒毛高度（VH）和VH /隐窝深度比最大。在84d时，与PP相比，VP提高了IL-8的表达，降低了存活率。总体而言，从生产角度来看，颗粒床上料适用于常规和有机方案下的速生肉鸡杂交品种。PP特别推荐用于有机生产系统。

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引用次数: 0

Effects of low vs high fat diet in dogs with Cerulein-induced acute pancreatitis 低脂与高脂饮食对蓝蛋白诱导的急性胰腺炎的影响

IF 1.8 3区农林科学 Q1 VETERINARY SCIENCES

Research in veterinary science

Pub Date : 2025-11-04 DOI: 10.1016/j.rvsc.2025.105951

Eszter Tünde Kanyorszky , Ágnes Sterczer , Nándor Balogh , Anna Szilasi , Petra Vad , Ferenc Manczur

Recommendations regarding the fat content of the diet used in treating canine acute pancreatitis (AP) are controversial. Generally, a low-fat diet is recommended, but this is primarily based on experts' opinions rather than clinical studies. Our study aimed to compare the effects of two feeding tube diets with different fat concentrations in an experimental model of AP. AP was induced by repeated cerulein injections in 10 beagle dogs, which were divided into two groups based on the fat content of their feeding tube diets (high-fat and low-fat). All other treatments were identical in the two groups. Clinical and laboratory parameters (including amylase, lipase, cPL, and CRP) and ultrasound findings were monitored for eight consecutive days. On the ninth day, the dogs were sacrificed to perform a detailed post-mortem examination, including a histological examination of their pancreas. The cerulein injection induced only mild AP in most of the dogs. The higher fat content of the diet did not cause a significant difference in the clinical, laboratory, ultrasound, and histological parameters of the dogs in this study. The resting energy requirements were not enough to fulfill the energy needs of the dogs with AP. Hepatic lipidosis was present in the low-fat group but was completely absent in the high-fat group.

Feeding a diet with a higher fat content during AP was well tolerated in this experimentally induced model of the disease. Further clinical research with different fat-containing diets is highly warranted in cases of spontaneously occurring AP.

关于治疗犬急性胰腺炎（AP）的饮食脂肪含量的建议是有争议的。一般来说，低脂饮食是推荐的，但这主要是基于专家的意见，而不是临床研究。本研究旨在比较两种不同脂肪浓度饲管饲料对急性胰腺炎实验模型的影响。通过反复注射蓝蛋白诱导10只beagle犬产生急性胰腺炎，并根据饲管饲料的脂肪含量（高脂和低脂）将其分为两组。所有其他治疗方法在两组中都是相同的。连续8天监测临床和实验室参数（包括淀粉酶、脂肪酶、cPL和CRP）和超声结果。在第九天，这些狗被处死进行详细的尸检，包括对它们的胰腺进行组织学检查。注射蓝蛋白后，大多数犬只出现轻度AP。在本研究中，饮食中较高的脂肪含量并没有引起狗的临床、实验室、超声和组织学参数的显著差异。静息能量需求不足以满足AP犬的能量需求。低脂组存在肝脂质沉积，而高脂组完全没有肝脂质沉积。在这种实验诱导的疾病模型中，在AP期间饲喂高脂肪含量的饮食是耐受良好的。进一步的临床研究，不同的含脂肪饮食的情况下，自发发生的AP是非常必要的。

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引用次数: 0

Comparison of premedication with or without vatinoxan, co-administered with medetomidine and methadone, in anesthetized dogs 麻醉犬用药前加或不加瓦替诺森与美托咪定和美沙酮联合用药的比较

IF 1.8 3区农林科学 Q1 VETERINARY SCIENCES

Research in veterinary science

Pub Date : 2025-11-04 DOI: 10.1016/j.rvsc.2025.105958

Luca Bellini , Francesca Zanusso , Lorena Lucatello , Beatrice Dussin , Francesca Capolongo

Vatinoxan, a peripheral alpha-2 antagonist, mitigates the cardiovascular effects of medetomidine but may cause hypotension during general anesthesia and alter the plasma concentrations of co-administered sedatives and analgesics. This study compares the effect of premedication with or without vatinoxan co-administered with medetomidine and methadone on sedation quality, cardiovascular and respiratory variables, and methadone plasma concentration in anesthetized dogs. Twenty healthy dogs undergoing elective ovariectomy were premedicated with methadone (0.2 mg/kg) and medetomidine (0.15 mg/m²), with vatinoxan (3 mg/m²) in group MV or without vatinoxan in group M. Sedation was assessed using a 21-point scale. Anesthesia was induced 20 min after and maintained with isoflurane. Cardiovascular and respiratory variables were recorded 15 min post-premedication, at traction on both ovarian pedicles, 90 min after premedication and at skin suture. Methadone plasma concentration was measured at 15, 90, and 240 min post-premedication. Sedation scores were significantly higher in group MV at 10 min post-premedication (p = 0.014). Only in group M, heart rate decreased significantly after premedication (p < 0.001). Vatinoxan administration did not affect intraoperative cardiovascular variables during nociceptive stimulation or in its absence. Hypotension was not observed in any dog. Time to recover from anesthesia and postoperative analgesia were not different between groups. Plasma methadone concentrations in group MV were not statistically different from group M, though slightly higher at 15 min post-premedication. For elective ovariectomy, adding vatinoxan to medetomidine and methadone provides rapid sedation, stable cardiovascular function during anesthesia, and smooth recovery causeing non-relevant variations in methadone plasma concentrations.

Vatinoxan是一种外周α -2拮抗剂，可减轻美托咪定对心血管的影响，但在全身麻醉时可能引起低血压，并改变镇静和镇痛药的血浆浓度。本研究比较了术前与美托咪定和美沙酮联合给药或不联合给药对麻醉犬的镇静质量、心血管和呼吸变量以及美沙酮血药浓度的影响。选取20只行择期卵巢切除术的健康犬，预先给予美沙酮（0.2 mg/kg）和美托咪定（0.15 mg/m2）， MV组加瓦替诺珊（3 mg/m2）， m组不加瓦替诺珊（3 mg/m2），采用21分制评估镇静作用。麻醉后20分钟诱导，异氟醚维持麻醉。记录用药前15分钟、牵引双卵巢蒂时、用药前90分钟和皮肤缝合时的心血管和呼吸变量。分别于用药前15、90、240 min测定美沙酮血药浓度。用药前10min时，MV组镇静评分显著高于对照组（p = 0.014）。只有M组用药前心率明显降低（p < 0.001）。在伤害性刺激或无伤害性刺激时，Vatinoxan给药不影响术中心血管变量。未见任何犬出现低血压。两组间麻醉恢复时间和术后镇痛时间无明显差异。MV组血浆美沙酮浓度与M组比较差异无统计学意义，用药前15 min时浓度略高于M组。择期卵巢切除术中，在美托咪定和美沙酮中加入瓦替诺珊可提供快速镇静，麻醉期间心血管功能稳定，恢复平稳，导致美沙酮血浆浓度发生非相关变化。

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引用次数: 0

Multi-epitope recombinant chimera and inactivated Escherichia coli expressing chimeric gene of Corynebacterium pseudotuberculosis as vaccines for caseous lymphadenitis 表达假结核棒状杆菌嵌合基因的多表位重组嵌合体和灭活大肠杆菌作为干酪性淋巴结炎疫苗

IF 1.8 3区农林科学 Q1 VETERINARY SCIENCES

Research in veterinary science

Pub Date : 2025-11-04 DOI: 10.1016/j.rvsc.2025.105956

Mara Thais de Oliveira Silva, Tallyson Nogueira Barbosa, Rodrigo Barros de Pinho, Luiza Domingues Moron, Nicole Ramos Scholl, Sibele Borsuk

The complete genome sequence of the FRC41 strain of Corynebacterium pseudotuberculosis allowing the identification of genes that code for proteins considered to be potential virulence factors (NanH, PknG, SodC and SpaC). In this study, a multi-epitope chimeric protein vaccine derived from these virulence factors was tested for efficacy in mice. Chimeric protein was expressed in Escherichia coli in purified and unpurified forms and used in immunization trial and challenge. Mice were immunized with a sterile 0.9 % saline solution (G1), recombinant chimera emulsified in Montanide ISA 61 (v/v) (G2), recombinant chimera emulsified in 15 % of adjuvant aluminum hydroxide (Al(OH)₃) (G3), inactivated Escherichia coli/chimera combined with Montanide (G4) and inactivated Escherichia coli/chimera combined with Al(OH)₃ (G5). Animals received two doses of vaccines with 21-days interval, and were challenge with Corinebacterium pseudotuberculosis MIC-6 strain. The total IgG, IgG1, and IgG2a production levels increased significantly in the experimental groups (G2-G5) from day 21. The IgG2a/IgG1 ratio suggests a Th1/Th2 immune response in all groups. The significant survival of 60 % was observed only in G5. The IFN-γ mRNA expression were up-regulated (p < 0.05) in G3, G4 and G5, the G4 and G5 also showed a significant increase in the IL-4 levels. On the other hand, only G3 presented significant higher levels of TNF-α in relation to control (G1). IL-12 expression was down-regulated (p < 0.05) in all experimental groups (G2-G5). Overall, the results obtained by G5 elicited a strong humoral and cellular immune response, highlighting its potential as a promising CLA vaccine candidate.

假结核棒状杆菌FRC41菌株的全基因组序列允许鉴定被认为是潜在毒力因子的蛋白质编码基因（NanH, pking， SodC和SpaC）。在这项研究中，由这些毒力因子衍生的多表位嵌合蛋白疫苗在小鼠身上进行了有效性测试。嵌合蛋白以纯化和未纯化两种形式在大肠杆菌中表达，并用于免疫试验和激射。用0.9%无菌盐水（G1）、Montanide ISA 61 (v/v) （G2）、15%佐剂氢氧化铝（Al(OH)3）（G3）、Montanide联合灭活大肠杆菌/嵌合体（G4）和Al(OH)3联合灭活大肠杆菌/嵌合体（G5）免疫小鼠。动物接种两剂疫苗，间隔21天，用假结核杆菌MIC-6菌株攻毒。从第21天开始，实验组（G2-G5）总IgG、IgG1和IgG2a分泌水平显著升高。IgG2a/IgG1比值提示所有组均有Th1/Th2免疫应答。仅在G5期观察到60%的显著生存率。G3、G4和G5的IFN-γ mRNA表达上调（p < 0.05）， G4和G5的IL-4水平也显著升高。另一方面，只有G3组的TNF-α水平明显高于对照组（G1）。各实验组（g2 ~ g5） IL-12表达均下调（p < 0.05）。总体而言，G5获得的结果引发了强烈的体液和细胞免疫反应，突出了其作为有希望的CLA候选疫苗的潜力。

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引用次数: 0

Characterization and immunogenicity of porcine adipose-derived stem cells 猪脂肪干细胞的特性和免疫原性

IF 1.8 3区农林科学 Q1 VETERINARY SCIENCES

Research in veterinary science

Pub Date : 2025-11-04 DOI: 10.1016/j.rvsc.2025.105954

Qiuyue Peng , Thomas Starch-Jensen , Benedict Kjærgaard , Trine Fink

Adipose-derived stem cells (ASCs) hold promise in regenerative medicine due to their multipotent differentiation capacity, anti-inflammatory effects, and ability to promote tissue repair. Porcine models are increasingly used in stem cell research due to their anatomical and physiological similarities to humans. This study aimed to characterize porcine ASCs (pASCs) and evaluate their immunogenicity following allogeneic transplantation. The pASCs were isolated from abdominal subcutaneous adipose tissue of an 18-month-old donor pig, expanded in an automated bioreactor, and cryopreserved. Characterization revealed high expression of mesenchymal markers (CD29, CD44, CD90, CD105) and absence of the hematopoietic marker CD45. The pASCs exhibited robust proliferative and self-renewal capacity, and the ability to differentiate into adipogenic, osteogenic, and chondrogenic lineages. Alloantibody analysis of 18 recipient pigs over four months post-transplantation demonstrated minimal alloantibody formation. These findings confirm mesenchymal stem cell identity and low immunogenicity of large-scale expanded pASCs, supporting their potential application in regenerative medicine. However, further studies are needed to validate the long-term therapeutic efficacy of pASCs in porcine disease models.

脂肪源性干细胞（ASCs）由于其多能分化能力、抗炎作用和促进组织修复的能力，在再生医学中有着广阔的应用前景。猪模型越来越多地用于干细胞研究，因为它们在解剖学和生理学上与人类相似。本研究旨在描述猪ASCs （pASCs）的特征，并评估其异体移植后的免疫原性。pASCs是从一只18个月大的供猪的腹部皮下脂肪组织中分离出来的，在自动生物反应器中扩增，并冷冻保存。表征显示间充质标志物（CD29、CD44、CD90、CD105）高表达，而造血标志物CD45缺失。pASCs表现出强大的增殖和自我更新能力，以及分化成脂肪、成骨和软骨谱系的能力。对18只移植后4个月的受体猪进行的同种异体抗体分析显示，同种异体抗体的形成很少。这些发现证实了大规模扩增pASCs的间充质干细胞特性和低免疫原性，支持其在再生医学中的潜在应用。然而，还需要进一步的研究来验证pASCs在猪疾病模型中的长期治疗效果。

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引用次数: 0