Research in veterinary science最新文献_第8页

Crosstalk between myostatin and callipyge in CRISPR/Cas9-edited goat fibroblast cells CRISPR/ cas9编辑山羊成纤维细胞中肌生成抑制素和callipyge之间的串扰。

IF 1.8 3区农林科学 Q1 VETERINARY SCIENCES

Research in veterinary science

Pub Date : 2025-11-26 DOI: 10.1016/j.rvsc.2025.105992

Helia Fathpour , Mansooreh Fouladi , Farnoosh Jafarpour , Reza Moradi-Hajidavaloo , Tayebeh Izadi , Hanieh Shiralian-Esfahani , Wilfried Kues , Mohammad-Hossein Nasr-Esfahani , Mehdi Hajian , Shahin Eghbalsaied

Myostatin (MSTN) and Callipyge (CLPG) genes are key regulators of muscle growth. While MSTN inhibits muscle development, the CLPG mutation induces muscle hypertrophy through a specific imprinted genetic mechanism. The interaction between these genes remains of interest for improving livestock muscle traits. In this study, CRISPR/Cas9 was employed to edit MSTN and CLPG genes in goat fibroblast cells via electrotransfection. Cells were selected using puromycin antibiotic, and gene-editing efficiency was evaluated through Sanger sequencing. Gene expression changes were analyzed using RT-qPCR analysis. MSTN gene knockout resulted in significant downregulation of MSTN and CLPG, while GTL2 expression was upregulated by more than 50-fold. Additionally, myosin heavy chain genes (MYH1, MYH3, MYH4) were strongly upregulated, with MYH3 13-fold and MYH4 30-fold increase in the expression. In CLPG-edited cells, the expression of MSTN, TRIM28, and CLPG was reduced, while GTL2 was upregulated by 6-fold. MYH3 and MYH4 expression increased 4-fold in CLPG-edited cells, though the increase was less pronounced compared to MSTN-edited cells. DLK1 expression was undetectable in both non-edited control and gene-edited fibroblast cells. Our findings support the interaction between MSTN and CLPG, contributing to the regulation of muscle growth. Notably, the study also highlights the challenges associated with editing imprinted genes like CLPG and suggests that TRIM28 may play a role downstream of CLPG regulation. These results provide valuable insights into muscle development regulation, offering potential applications in livestock genetic improvement.

肌生长抑制素（MSTN）和Callipyge （CLPG）基因是肌肉生长的关键调控因子。虽然MSTN抑制肌肉发育，但CLPG突变通过特定的印迹遗传机制诱导肌肉肥大。这些基因之间的相互作用仍然是改善牲畜肌肉性状的兴趣。本研究利用CRISPR/Cas9对山羊成纤维细胞的MSTN和CLPG基因进行电转染编辑。使用嘌呤霉素抗生素筛选细胞，通过Sanger测序评估基因编辑效率。采用RT-qPCR分析基因表达变化。MSTN基因敲除导致MSTN和CLPG显著下调，GTL2表达上调50倍以上。此外，肌凝蛋白重链基因（MYH1、MYH3、MYH4）的表达显著上调，其中MYH3和MYH4的表达分别增加了13倍和30倍。在CLPG编辑的细胞中，MSTN、TRIM28和CLPG的表达减少，而GTL2的表达上调了6倍。在clpg编辑的细胞中，MYH3和MYH4的表达增加了4倍，尽管与mstn编辑的细胞相比，增加不那么明显。在未编辑的对照和基因编辑的成纤维细胞中均检测不到DLK1的表达。我们的研究结果支持MSTN和CLPG之间的相互作用，有助于调节肌肉生长。值得注意的是，该研究还强调了与编辑CLPG等印迹基因相关的挑战，并表明TRIM28可能在CLPG调控的下游发挥作用。这些结果为肌肉发育调控提供了有价值的见解，为牲畜遗传改良提供了潜在的应用。

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引用次数: 0

Effects of dietary supplementation of an oleuropein-enriched olive leaf extract on performance, carcass characteristics, intestinal health, foot pad parameters and meat quality of broilers 饲粮中添加富含橄榄苦苷的橄榄叶提取物对肉鸡生产性能、胴体特性、肠道健康、脚垫参数和肉品质的影响

IF 1.8 3区农林科学 Q1 VETERINARY SCIENCES

Research in veterinary science

Pub Date : 2025-11-25 DOI: 10.1016/j.rvsc.2025.105990

Konstantina Vasilopoulou , Georgios A. Papadopoulos , Styliani Lioliopoulou , Leandros Skaltsounis , Panagiotis Stathopoulos , Ioanna Stylianaki , Dimitrios Galamatis , Vasileios Tsiouris , Georgios Arsenos , Ilias Giannenas

Incorporating agricultural by-products into animal diets represents a sustainable strategy to enhance animal health and product quality. Olive leaves, rich in bioactive compounds such as oleuropein, exhibit notable antioxidant, antimicrobial, and anti-inflammatory properties. This study evaluated the effects of dietary supplementation with an environmentally friendly, oleuropein-enriched OLE (OLE) on broiler chickens. A total of 480 day-old chicks were randomly assigned to four groups: a control diet - CF (T1), CF + 1 % OLE (T2), CF + 2.5 % OLE (T3), and CF + 0.1 % encapsulated oregano oil (T4, positive control). Over 42 days, growth performance, carcass characteristics, intestinal morphology, foot pad health, and meat quality were assessed. While T4 showed superior growth performance, the 1 % OLE diet significantly improved intestinal morphology and meat quality. A dose-dependent yellow pigmentation of the foot pad was observed in OLE-treated groups. Additionally, breast tenderness and darker thigh coloration improved in OLE-fed broilers. These findings suggest that 1 % OLE can be a viable natural additive to support gut health and meat quality in broilers, with comparable antioxidant effects to oregano oil and no adverse outcomes.

将农业副产品纳入动物饲料是提高动物健康和产品质量的可持续战略。橄榄叶富含橄榄苦苷等生物活性化合物，具有显著的抗氧化、抗菌和抗炎特性。本研究评价了饲粮中添加一种富含橄榄苦苷的环保型OLE （OLE）对肉鸡的影响。选取480日龄雏鸡，随机分为4组：对照饲粮- CF （T1）、CF + 1% OLE （T2）、CF + 2.5% OLE （T3）和CF + 0.1%胶囊牛至油（T4，阳性对照）。在42 d内，对生长性能、胴体特性、肠道形态、脚垫健康和肉品质进行评估。虽然T4表现出优异的生长性能，但1% OLE日粮显著改善了肠道形态和肉品质。在ole处理组中观察到足垫的剂量依赖性黄色色素沉着。此外，ole饲养肉鸡的乳房柔软度和大腿颜色较深有所改善。这些研究结果表明，1% OLE是一种可行的天然添加剂，可支持肉鸡肠道健康和肉品质，具有与牛至油相当的抗氧化效果，且无不良后果。

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引用次数: 0

Serum iron levels as a biomarker for monitoring fracture healing in dogs: A longitudinal study 血清铁水平作为监测狗骨折愈合的生物标志物：一项纵向研究

IF 1.8 3区农林科学 Q1 VETERINARY SCIENCES

Research in veterinary science

Pub Date : 2025-11-25 DOI: 10.1016/j.rvsc.2025.105993

Ersoy Baydar , Ugur Aydogdu , Feyyaz Kaya , Muharrem Erol

Iron is an important mineral involved in various physiological processes and can be used as a biomarker, since its serum concentration changes during the inflammatory response. Eight crossbreed dogs with closed fractures in the antebrachium bone were included in the study. The fracture was treated with a closed reduction method and blood samples were taken initially at 0 h and subsequently at 7, 14, 21, 28, and 45 days. Iron, ferritin, hepcidin, tumor necrosis factor-alpha (TNF-α), C reactive protein (CRP), bone alkaline phosphatase (BALP), osteocalcin, total antioxidant status (TAS), total oxidant status (TOS) as well as routine hemogram and biochemistry analyses were performed in the blood samples taken. It was determined that the decrease in serum Fe levels reversed as the process progressed even though hepcidin, ferritin, osteocalcin, BALP, and TNF-α levels increased at the beginning of the healing process. As a result, it can be suggested that the analysis of serum Fe levels may be a useful biomarker in monitoring the fracture healing process.

铁是一种重要的矿物质，参与多种生理过程，可以作为生物标志物，因为它的血清浓度在炎症反应中发生变化。8只前臂骨闭合性骨折的杂交犬被纳入研究。采用闭合复位方法治疗骨折，并于第0小时、第7天、第14天、第21天、第28天和第45天采集血样。采集血样进行铁、铁蛋白、肝磷脂、肿瘤坏死因子-α (TNF-α)、C反应蛋白（CRP）、骨碱性磷酸酶（BALP）、骨钙素、总抗氧化状态（TAS）、总氧化状态（TOS）以及常规血象和生化分析。结果表明，尽管hepcidin、铁蛋白、骨钙素、BALP和TNF-α水平在愈合过程开始时升高，但随着愈合过程的进展，血清铁水平的下降趋势逆转。结果表明，血清铁水平的分析可能是监测骨折愈合过程中有用的生物标志物。

引用次数: 0

Lactobacilli isolated from chickens of Italian native breeds: Antimicrobial susceptibility profile, probiotic potential and anti-Salmonella activity 意大利地方品种鸡的乳酸菌分离：抗菌敏感性、益生菌潜力和抗沙门氏菌活性

IF 1.8 3区农林科学 Q1 VETERINARY SCIENCES

Research in veterinary science

Pub Date : 2025-11-24 DOI: 10.1016/j.rvsc.2025.105988

Ilaria Resci , Marta Raffaelli , Francesca Pedonese , Mario Forzan , Filippo Fratini , Sonia Salvucci , Beatrice Torracca , Margherita Marzoni , Barbara Turchi

Salmonellosis is the second most prevalent foodborne disease in Europe, with eggs, mixed foods, and broiler meat being the primary sources of transmission. Among human salmonellosis cases, the most prevalent Salmonella enterica serovars are S. Enteritidis, S. Typhimurium, and monophasic S. Typhimurium. However, S. Infantis and S. Newport are the most frequently detected serovars in broiler and turkey meat, respectively. Over time, Salmonella spp. have developed multidrug resistance, and the global rise of antimicrobial resistance has driven the search for alternative strategies to antimicrobials use. Probiotics represent a promising alternative to antimicrobials, with the added benefit of enhancing production performance. This study aimed to isolate lactobacilli from chickens of Italian native breeds to evaluate their antimicrobial susceptibility, probiotic potential (tolerance to acidic pH and bile salts), and anti-Salmonella activity against S. enterica strains previously isolated from poultry meat. Antimicrobial susceptibility testing was carried out on all 205 obtained isolates as a preliminary screening. Only nine isolates (4.4 %) were susceptible to all tested antimicrobials. Among them, one strain exhibited notable probiotic potential and anti-Salmonella activity. Lactobacillus kitasatonis VALFI55 demonstrated survival at acidic pH for up to 120 min and tolerance to bile salt concentrations of 0.3 % and 1 %, with limited reduction in viable cell counts compared to other isolates. Furthermore, L. kitasatonis VALFI55 exhibited the strongest inhibitory effect, particularly against S. Typhimurium and S. Infantis. The VALFI55 strain represents a promising probiotic candidate for application in poultry breeding. However, further in vivo studies are required to confirm its beneficial effects.

沙门氏菌病是欧洲第二大流行食源性疾病，鸡蛋、混合食品和肉鸡肉是主要传播源。在人类沙门氏菌病病例中，最常见的肠沙门氏菌血清型是肠炎沙门氏菌、鼠伤寒沙门氏菌和单相鼠伤寒沙门氏菌。然而，S. Infantis和S. Newport分别是肉鸡和火鸡肉中最常检测到的血清型。随着时间的推移，沙门氏菌已经产生了多药耐药性，全球抗菌素耐药性的上升促使人们寻找替代抗菌素使用的策略。益生菌是一种很有前途的抗菌剂替代品，具有提高生产性能的额外好处。本研究旨在从意大利本土品种的鸡中分离乳酸菌，以评估其抗菌敏感性、益生菌潜力（对酸性pH和胆汁盐的耐受性）以及对先前从禽肉中分离到的肠炎沙门氏菌菌株的抗沙门氏菌活性。对获得的205株菌株进行了药敏试验作为初步筛选。只有9株（4.4%）对所有测试的抗菌素敏感。其中1株菌株表现出显著的益生菌潜力和抗沙门氏菌活性。与其他分离株相比，kitasatonlactobacillus VALFI55在酸性pH下存活长达120分钟，对胆盐浓度为0.3%和1%的耐受性有限，活细胞计数减少有限。此外，L. kitasatonis VALFI55对鼠伤寒沙门氏菌和婴儿沙门氏菌的抑制作用最强。VALFI55菌株是一种很有希望在家禽育种中应用的候选益生菌。然而，需要进一步的体内研究来证实其有益作用。

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引用次数: 0

In silico characterization of the OSBPL6 gene and its potential role in ascites syndrome in broiler chickens OSBPL6基因在肉鸡腹水综合征中的作用

IF 1.8 3区农林科学 Q1 VETERINARY SCIENCES

Research in veterinary science

Pub Date : 2025-11-24 DOI: 10.1016/j.rvsc.2025.105986

M.R. Emami, M. Najafi

Ascites syndrome is a multifactorial metabolic disorder in broiler chickens, characterized by pulmonary hypertension, fluid accumulation, and high mortality, leading to significant economic losses. The oxysterol-binding protein-like 6 gene (OSBPL6) has been associated with ascites susceptibility, but its molecular role remains unclear. Therefore, this study was aimed to perform a comprehensive in-silico analysis of chicken OSBPL6, including analyses of physicochemical properties, structural features, post-translational modifications, protein–protein interactions, promoter motifs, and tissue-specific expression to elucidate its involvement in ascites syndrome pathogenesis in broiler chickens. OSBPL6 encodes a 978-amino acid protein with a molecular mass of 110,905.88 Da, exhibiting predominantly hydrophilic characteristics (GRAVY = −0.583), moderate thermostability (aliphatic index = 76.45), and predicted instability in vitro (instability index = 65.47). Analysis revealed 134 phosphorylation sites and 6 glycosylation sites, indicating extensive post-translational regulation. Subcellular localization predictions suggested cytoplasmic residence with peripheral membrane associations and potential nucleocytoplasmic shuttling capability. Conserved PH, ORD, and FFAT domains were identified, supporting roles in sterol transport and lipid binding functions. Among structural models generated, trRosetta showed the highest reliability (QMEANDisCo =0.66, ERRAT = 94.3 %). Protein–protein interaction analysis linked OSBPL6 to pathways related to cardiac hypertrophy, muscle metabolism, and oxidative stress responses. Promoter analysis identified transcription factor binding sites linked to vascular remodeling, myocardial hypertrophy, and muscle development. Expression profiling showed highest OSBPL6 expression in skeletal muscle and elevated levels in cardiac tissue, consistent with ascites-associated pathophysiology. This integrative analysis highlights OSBPL6 as a candidate regulator of muscle growth and cardiopulmonary remodeling, with potential as a biomarker for genetic selection against ascites in broiler chickens.

腹水综合征是肉鸡的一种多因素代谢紊乱，以肺动脉高压、积液和高死亡率为特征，导致重大经济损失。氧甾醇结合蛋白样6基因（OSBPL6）与腹水易感性有关，但其分子作用尚不清楚。因此，本研究旨在对鸡OSBPL6进行全面的计算机分析，包括理化性质、结构特征、翻译后修饰、蛋白-蛋白相互作用、启动子基序和组织特异性表达分析，以阐明其在肉鸡腹水综合征发病机制中的作用。OSBPL6编码一个978个氨基酸的蛋白，分子量为110,905.88 Da，具有明显的亲水性（肉卤=−0.583），中等热稳定性（脂肪族指数= 76.45），体外不稳定性（不稳定性指数= 65.47）。分析发现134个磷酸化位点和6个糖基化位点，表明广泛的翻译后调控。亚细胞定位预测表明细胞质与外周膜的联系和潜在的核细胞质穿梭能力。发现了保守的PH、ORD和FFAT结构域，它们在固醇转运和脂质结合功能中起支持作用。在生成的结构模型中，trRosetta的可靠性最高（QMEANDisCo =0.66, ERRAT = 94.3%）。蛋白-蛋白相互作用分析将OSBPL6与心脏肥厚、肌肉代谢和氧化应激反应相关的途径联系起来。启动子分析确定了与血管重构、心肌肥大和肌肉发育相关的转录因子结合位点。表达谱显示OSBPL6在骨骼肌中表达最高，在心脏组织中表达水平升高，与腹水相关的病理生理一致。这项综合分析强调了OSBPL6作为肌肉生长和心肺重塑的候选调节因子，具有作为肉仔鸡抗腹水遗传选择的生物标志物的潜力。

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引用次数: 0

Main Salmonella serotypes in free-range Amazon broiler chicken farms: Comparison between collection methods and seasonal period in tropical regions 亚马逊散养肉鸡养殖场主要沙门氏菌血清型：热带地区收集方法与季节的比较

IF 1.8 3区农林科学 Q1 VETERINARY SCIENCES

Research in veterinary science

Pub Date : 2025-11-24 DOI: 10.1016/j.rvsc.2025.105987

Hellen N.C.M. Miranda , Alessandro J.F. dos Santos , Katyane de S. Almeida , José C. Ribeiro-Júnior

Broiler chicken raised in semi-closed or completely free-range systems are challenged by more environmental variables that can compromise the biosecurity and microbiological quality and safety of the final product from this type of production. This study investigated Salmonella serotypes in Brazilian Amazon free-range broiler chicken farms in a tropical climate region, comparing the efficacy of the official collection methods of poultry sheds (drag swabs, footpads, and cecal feces) and the rainy and dry seasons typical of the region. Specific qualitative microbiological cultures, confirmation of identity by genus-specific PCR (invA), and determination of Salmonella serotypes by real-time PCR were performed on all free-range poultry farms in Tocantins, North Brazil. In total, 935 suggestive isolates were recovered using the three collection methods in both seasons, of which 90 (9.5 %) were positive for invA. There were no significant differences (p > 0.05) between the collection methods or between the rainy and dry seasons. Salmonella Mbandaka, S. Panama, and S. Javiana were identified on different free-range poultry farms. Although the main serotypes of importance in poultry and public health were not identified, according to Brazilian regulations, the results did not compromise the processing of batches for fresh trade, reinforcing the need to support surveillance programs, animal health defense, and epidemiological measures of a one-health approach to control the pathogen in the chain of free-range broiler chickens raised in tropical climate regions.

在半封闭或完全自由放养系统中饲养的肉鸡面临更多环境变量的挑战，这些环境变量可能会损害这种生产方式的最终产品的生物安全和微生物质量和安全。本研究调查了热带气候地区巴西亚马逊散养肉鸡养殖场的沙门氏菌血清型，比较了官方家禽棚收集方法（拖拭子、脚垫和盲肠粪便）和该地区典型的雨季和旱季的效果。在巴西北部Tocantins的所有散养家禽养殖场进行了特异性定性微生物培养、属特异性PCR （invA）鉴定和实时PCR检测沙门氏菌血清型。两季三种采集方法共检出935株提示分离株，其中90株（9.5%）为invA阳性。不同采集方式间、旱季与雨季间无显著差异（p > 0.05）。在不同的散养家禽养殖场发现了姆班达卡沙门氏菌、巴拿马沙门氏菌和贾维亚纳沙门氏菌。根据巴西法规，虽然没有确定对家禽和公共卫生具有重要意义的主要血清型，但结果并没有影响新鲜贸易批次的加工，这加强了支持监测计划、动物健康防御和单一健康方法的流行病学措施的必要性，以控制热带气候地区饲养的散养肉鸡链中的病原体。

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引用次数: 0

Organoid-based platforms in livestock: Current advances and future prospects 家畜类器官平台：当前进展与未来展望

IF 1.8 3区农林科学 Q1 VETERINARY SCIENCES

Research in veterinary science

Pub Date : 2025-11-23 DOI: 10.1016/j.rvsc.2025.105985

Dong-Hyeok Kwon , Hanbyul Kwon , Goo Jang

Organoid research has been extensively developed and investigated in humans and rodents; however, its applications in livestock species remain comparatively limited. To address this knowledge gap, this review provides a comprehensive synthesis of the historical evolution, establishment protocols, and current organ-specific applications of livestock-derived organoids, spanning tissues such as the intestine, liver, lung, kidney, testis, mammary gland, and reproductive tract. These self-organizing three-dimensional cultures serve as powerful platforms for investigating fundamental questions in veterinary science, from modeling host–pathogen interactions in infectious diseases to exploring the complex biology of reproduction, toxicology, and productivity. Additionally, recent advances in bioengineering and genome-editing technologies, including CRISPR/Cas9, base editing, and prime editing, are highlighted for their potential to enhance the physiological relevance and reproducibility of livestock organoid systems. Despite ongoing challenges, including the lack of standardized protocols, limited availability of pluripotent stem cell–derived models, and insufficient multicellular complexity, livestock organoids represent a strategically important frontier in veterinary science and agricultural biotechnology. In conclusion, the combination of stem cell biology, microphysiological platforms, and genetic engineering positions livestock organoids as indispensable tools for veterinary medicine, agricultural innovation, and translational biomedical research.

类器官研究在人类和啮齿类动物中得到了广泛的发展和研究；然而，其在家畜中的应用仍然相对有限。为了解决这一知识缺口，本文综述了家畜源性类器官的历史演变、建立方案和当前器官特异性应用的全面综合，涵盖肠、肝、肺、肾、睾丸、乳腺和生殖道等组织。这些自组织的三维培养为研究兽医科学的基本问题提供了强大的平台，从传染病中宿主-病原体相互作用的建模到探索繁殖、毒理学和生产力的复杂生物学。此外，生物工程和基因组编辑技术的最新进展，包括CRISPR/Cas9、碱基编辑和引物编辑，因其增强家畜类器官系统的生理相关性和可重复性的潜力而得到强调。尽管目前存在挑战，包括缺乏标准化协议、多能干细胞衍生模型的可用性有限、多细胞复杂性不足，但牲畜类器官代表了兽医科学和农业生物技术的一个重要战略前沿。总之，干细胞生物学、微生理平台和基因工程的结合使牲畜类器官成为兽医学、农业创新和转化生物医学研究不可或缺的工具。

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引用次数: 0

Incidental amplification of Wolbachia sequences in fleas (Siphonaptera: Pulicidae) collected from pets in Thailand using 16S rDNA universal primers for hemoplasma detection 用16S rDNA通用引物扩增泰国宠物蚤（虹吸翅目：蚤科）沃尔巴克氏体序列。

IF 1.8 3区农林科学 Q1 VETERINARY SCIENCES

Research in veterinary science

Pub Date : 2025-11-23 DOI: 10.1016/j.rvsc.2025.105984

Kritsada Thongmeesee , Aung Aung , Duriyang Narapakdeesakul , Patchana Kamkong , Apinya Chatchaisuriya , Phichsita Satidsommon , Sarun Kerdrojwongkul , Sasikan Lilertlam , Sukritta Charoensiri , Sonthaya Tiawsirisup

Fleas (Siphonaptera: Pulicidae) are common ectoparasites of cats and dogs in Thailand, and they can serve as vectors for various pathogens with zoonotic potential [e.g., Bartonella spp., Rickettsia spp., and hemotropic Mycoplasma spp. (hemoplasmas)]. Bartonella and Rickettsia spp. have been well described in fleas collected from cats and dogs. Contrarily, only one study reported hemoplasmas in fleas collected from cats in Thailand, and information regarding hemoplasmas in fleas collected from dogs is limited. This study used 16S rDNA universal primers in a previous study to investigate hemoplasmas in fleas collected from cats and dogs. Unfortunately, the primers inadvertently amplified the partial sequences of Wolbachia spp. endosymbionts in fleas. All obtained sequences were analyzed using nucleotide BLAST and phylogenetic analysis. Based on the results, the Wolbachia sequences were placed into two subsupergroups (I-1 and I-2) with sequences from Ctenocephalides felis and C. orientis. Although these 16S rDNA primers used in this study might be capable of amplifying hemoplasmas in mammalian hosts, additional Sanger sequencing might be required to confirm PCR products as hemoplasma sequences when these primers are used for cPCR in arthropod samples.

跳蚤（管翅目：蚤科）是泰国常见的猫和狗的体表寄生虫，它们可以作为各种具有人畜共患潜力的病原体的媒介[例如巴尔通体、立克次体和嗜血血支原体（血浆）]。在从猫和狗身上收集的跳蚤中已很好地描述了巴尔通体和立克次体。相反，只有一项研究报告了在泰国从猫身上收集的跳蚤中存在血浆，而从狗身上收集的跳蚤中存在血浆的信息有限。本研究使用先前研究中的16S rDNA通用引物来调查从猫和狗身上收集的跳蚤的血浆。不幸的是，引物无意中扩增了蚤体内沃尔巴克氏体内共生体的部分序列。所有获得的序列用核苷酸BLAST和系统发育分析进行分析。基于上述结果，将沃尔巴克氏体序列与猫头线虫和东方线虫的序列划分为两个亚超群（I-1和I-2）。虽然本研究中使用的这些16S rDNA引物可能能够扩增哺乳动物宿主的血浆，但当这些引物用于节肢动物样本的cPCR时，可能需要额外的Sanger测序来确认PCR产物是血浆序列。

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引用次数: 0

Comparative histologic survey and transcriptomic investigation into canine prostate carcinoma 犬前列腺癌的比较组织学调查和转录组学研究

IF 1.8 3区农林科学 Q1 VETERINARY SCIENCES

Research in veterinary science

Pub Date : 2025-11-19 DOI: 10.1016/j.rvsc.2025.105981

Nathan K. Hoggard , Said M. Elshafae , Nigel A. Daniels , Jonathan A. Young , Chris Premanandan , John B. Echols , Darshan S. Chandrashekar , Blake E. Hildreth III , Michael C. Haffner , Thomas J. Rosol

Dogs share features in prostate gland anatomy, physiology, and pathology with men. However, human and canine prostate carcinoma (PC) have histologic and molecular differences. Particularly, the histogenesis of canine PC (cPC) is unclear. This study investigated the origin of cPC using histopathology and transcriptomics with comparison to men. Prostate glands retrospectively and prospectively collected from 445 dogs (approximately 95 % autopsy samples) were surveyed for early carcinomas and preneoplastic lesions, particularly high-grade prostatic intraepithelial neoplasia (HGPIN) due to its role in the pathogenesis of PC in men. Lineage gene signatures defining prostate luminal epithelium and urothelium were identified for inter- and intraspecies RNA-sequencing comparisons, including between cPC and canine urinary bladder urothelial carcinoma (UC). Postmortem prostate lesion frequencies were similar to previously reported canine studies. Intraductal/intra-acinar growth (31/35; 88.6 %) was common in representative samples of cPC. Prostate epithelial changes consistent with HGPIN in men were not observed. Proliferative lesions and early carcinomas were rare (7/445; 1.6 %). Patterns in prostate and urothelium marker gene expression signatures differed between human and canine PC. Compared to non-neoplastic prostate gland, cPC had significantly decreased prostate-specific and increased urothelium gene signatures. The results suggest many cases diagnosed as cPC are UC or have urothelial differentiation and thus differ from PC in men, with important implications for canine tumor classification and translational studies.

狗在前列腺解剖、生理和病理上与人有共同的特点。然而，人和犬前列腺癌（PC）具有组织学和分子差异。特别是犬PC （cPC）的组织发生尚不清楚。本研究利用组织病理学和转录组学研究了cPC的起源，并与男性进行了比较。回顾性和前瞻性地收集了445只狗的前列腺（约95%的尸检样本），调查了早期癌和癌前病变，特别是高级别前列腺上皮内瘤变（HGPIN），因为它在男性PC的发病机制中起作用。确定了定义前列腺管腔上皮和尿路上皮的谱系基因特征，用于种间和种内rna测序比较，包括cPC和犬膀胱尿路上皮癌（UC）之间的rna测序比较。死后前列腺病变频率与先前报道的犬类研究相似。导管内/腺泡内生长（31/35,88.6%）在cPC的代表性样本中很常见。在男性中未观察到与HGPIN一致的前列腺上皮改变。增殖性病变和早期癌罕见（7/445;1.6%）。前列腺和尿路上皮标记基因表达特征的模式在人和犬PC之间存在差异。与非肿瘤性前列腺相比，cPC显著降低了前列腺特异性，增加了尿路上皮基因特征。结果提示，许多诊断为cPC的病例为UC或有尿路上皮分化，因此与男性PC不同，这对犬肿瘤的分类和转化研究具有重要意义。

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引用次数: 0

Evaluation of hair samples for PCR detection of Leishmania infantum in dogs 犬毛标本PCR检测幼年利什曼原虫的评价

IF 1.8 3区农林科学 Q1 VETERINARY SCIENCES

Research in veterinary science

Pub Date : 2025-11-19 DOI: 10.1016/j.rvsc.2025.105979

Geovanna Vioti , João Augusto Franco Leonel , Trícia Maria Ferreira de Sousa Oliveira , Diogo Tiago da Silva , Maria Luana Alves , Andrea Cristina Higa Nakaghi , Lara Borges Keid , Rodrigo Martins Soares

Canine leishmaniosis (CanL) caused by Leishmania infantum remains a major public health concern in endemic regions, where dogs are the primary domestic reservoir. Despite high accuracy, the invasiveness of current diagnostic methods limits their practical application. This study evaluated the diagnostic potential of hair as a non-invasive sample for molecular detection of L. infantum using a nested PCR targeting the ITS1 region (HAIR-test). All PCR-based tests in this study, including those performed on lymph node aspirates (LN-test), blood (BL-test), and conjunctival swabs (CS-test), employed the same nested ITS1-PCR protocol. A cohort of 44 naturally infected dogs, defined by parasitological confirmation or LN-test positivity, was evaluated using the HAIR-test, with hair samples independently collected by two different operators. The combined sensitivity of the HAIR-test was 59.1 %, outperforming the BL-test and matching the performance of the CS-test. Sequencing confirmed L. infantum DNA in hair samples, but also revealed co-amplification of non-Leishmania trypanosomatids in a subset, underscoring the need for high-specificity assays. Inter-collector agreement was moderate, indicating potential variability in parasite distribution or inconsistencies in sample collection and handling. No correlation was observed between hair mass and PCR-positivity, suggesting that L. infantum DNA is present in follicular bulbs rather than in hair shafts. Beyond suboptimal sensitivity, limitations in assay reproducibility and uncertainties regarding the precise origin of the detected DNA underscore the need for further methodological refinement and validation. The HAIR-test offers a viable, non-invasive alternative in settings where invasive sampling is impractical, but its routine diagnostic application requires methodological refinements and validation.

由幼年利什曼原虫引起的犬利什曼病（CanL）在流行地区仍然是一个主要的公共卫生问题，在这些地区，狗是主要的家养宿主。尽管准确率很高，但目前的诊断方法的侵入性限制了它们的实际应用。本研究利用针对ITS1区域的巢式PCR方法（hair -test）评估了毛发作为无创样本对婴儿乳杆菌分子检测的诊断潜力。本研究中所有基于pcr的检测，包括对淋巴结抽吸液（LN-test）、血液（BL-test）和结膜拭子（CS-test）进行的检测，均采用相同的巢式ITS1-PCR方案。通过寄生虫学确认或ln测试阳性确定的44只自然感染狗，使用毛发测试进行评估，毛发样本由两个不同的操作人员独立收集。HAIR-test的综合灵敏度为59.1%，优于BL-test，与CS-test相当。测序证实了头发样本中的婴儿乳杆菌DNA，但也显示了非利什曼岛锥虫虫在一个亚群中的共扩增，强调了高特异性检测的必要性。收集者之间的一致性中等，表明寄生虫分布的潜在变异性或样品收集和处理的不一致。毛质量与pcr阳性之间没有相关性，这表明婴儿乳杆菌DNA存在于毛囊球茎中而不是毛干中。除了灵敏度不够理想之外，检测重现性的限制和检测DNA精确来源的不确定性强调了进一步改进和验证方法的必要性。在侵入性取样不切实际的情况下，毛发测试提供了一种可行的、非侵入性的替代方法，但其常规诊断应用需要方法的改进和验证。

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引用次数: 0