Research in veterinary science最新文献_第8页

Integrated analysis of transcriptome and metabolome reveal the complex molecular regulatory network of chicken house PM2.5 induced lung injury 转录组和代谢组的综合分析揭示了鸡舍PM2.5诱导肺损伤的复杂分子调控网络。

IF 1.8 3区农林科学 Q1 VETERINARY SCIENCES

Research in veterinary science

Pub Date : 2026-03-01 Epub Date: 2025-12-22 DOI: 10.1016/j.rvsc.2025.106039

Bo Wu , Wendan Zheng , Yu Bai , Hongrui Huang , Tongjie Chai

Fine particulate matter (PM2.5) in poultry houses is the main component of air pollution. Long-term exposure to high concentrations of PM2.5 could easily damage the respiratory system of animals. In this study, we first investigated the changes in lung tissue morphology and serum cytokines in SPF chickens after exposure to poultry house PM2.5 and further conduct a combined analysis of its lung transcriptome and metabolome. The results showed that chicken exposed to PM2.5 led to decrease in body weight and cause inflammatory damage such as pulmonary fibrosis in chickens. It also significantly upregulated the expression levels of serum cytokines IL-1β, IL-6, TLR-2, TLR-4, IFN-α, TGF-β and CCL2. Additionally, it affected Arachidonic acid metabolism, Ferroptosis, and mTOR signaling pathways, disrupting metabolic processes and the expression of genes related to pulmonary inflammation. In addition, we validated the results of the multi-omics combined analysis and found that the interaction between the differential metabolite Arachidonic acid and the three differentially expressed genes LTA4H, FTL and PLA2G4A led to pulmonary inflammatory damage and metabolic disorders. The above results indicate that the interaction between pulmonary inflammation-related genes and lung metabolites may play a crucial role in the process of PM2.5-induced lung injury in chickens.

鸡舍内的细颗粒物（PM2.5）是空气污染的主要组成部分。长期暴露在高浓度的PM2.5中，很容易损害动物的呼吸系统。在本研究中，我们首先研究SPF鸡暴露于鸡舍PM2.5后肺组织形态和血清细胞因子的变化，并进一步对其肺转录组和代谢组进行联合分析。结果表明，暴露在PM2.5环境中的鸡体重下降，并引起肺纤维化等炎症性损伤。显著上调血清细胞因子IL-1β、IL-6、TLR-2、TLR-4、IFN-α、TGF-β、CCL2的表达水平。此外，它还影响花生四烯酸代谢、铁下垂和mTOR信号通路，破坏代谢过程和与肺部炎症相关的基因表达。此外，我们验证了多组学联合分析的结果，发现差异代谢物花生四烯酸与三个差异表达基因LTA4H、FTL和PLA2G4A之间的相互作用导致肺部炎症损伤和代谢紊乱。上述结果表明，肺炎症相关基因与肺代谢物的相互作用可能在pm2.5诱导的鸡肺损伤过程中起着至关重要的作用。

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引用次数: 0

Zoledronate-induced activation of γδ T cells is associated with NK cell activation and reduced parasite burden in the cecum of Eimeria tenella-infected chicks 唑来膦酸钠诱导的γδ T细胞活化与幼美耳球虫感染雏鸡盲肠内NK细胞活化和寄生虫负荷减少有关。

IF 1.8 3区农林科学 Q1 VETERINARY SCIENCES

Research in veterinary science

Pub Date : 2026-03-01 Epub Date: 2025-12-17 DOI: 10.1016/j.rvsc.2025.106030

Quan Viet Le , Makoto Matsubayashi , Toshimitsu Hatabu

This study aimed to elucidate the role of γδ T cells in chicks infected with Eimeria tenella. Zoledronate was administered in vivo to activate γδ T cells. White Leghorn chicks were divided into four groups: zoledronate-treated (ZOL), infected control (CT), uninfected zoledronate-treated (UN/ZOL), and uninfected control (UN). Chicks in the CT and ZOL groups were orally inoculated with sporulated E. tenella oocysts (1 × 10^4 oocysts/chick) at 14 days of age. Fecal samples were collected between 4 and 15 days post-infection (dpi) to assess oocyst shedding. Cecal samples were obtained at 5 dpi for histopathological examination and gene expression analysis. Lesion and parasite burden scores were significantly reduced in the ZOL group compared with the CT group. Expression levels of IL-17 A, IL-21, and IFN-γ were significantly higher in the ZOL group, whereas IL-13 and PGES expression was significantly higher in the CT group. These findings indicate that activation of γδ T cells alleviates cecal tissue damage caused by E. tenella infection. Moreover, they suggest that NK cells activated by IL-21 may play a role in parasite control and contribute to the establishment of the local immune environment, but the effect of parasite control by IL-21-induced NK cell activation requires further research. Future studies should also explore whether γδ T cell activation and IL-21-mediated immunity are associated with mechanisms of parasite control.

本研究旨在阐明γδ T细胞在雏鸡感染柔嫩艾美耳球虫中的作用。体内给药唑来膦酸钠活化γδ T细胞。将来港白雏鸡分为唑来膦酸盐处理组（ZOL）、感染对照组（CT）、未感染唑来膦酸盐处理组（UN/ZOL）和未感染对照组（UN） 4组。CT组和ZOL组雏鸡在14日龄时口服带孢子的柔嫩E.卵囊（1 × 10^4个/只）。在感染后4至15天收集粪便样本以评估卵囊脱落情况。5 dpi时取盲肠标本进行组织病理学检查和基因表达分析。与CT组相比，ZOL组的病变和寄生虫负担评分明显降低。ZOL组IL-17 A、IL-21、IFN-γ表达水平显著高于对照组，而CT组IL-13、PGES表达水平显著高于对照组。这些结果表明，γδ T细胞的活化可减轻tenella感染引起的盲肠组织损伤。此外，他们认为IL-21活化的NK细胞可能在寄生虫控制中发挥作用，有助于建立局部免疫环境，但IL-21诱导的NK细胞活化对寄生虫的控制效果还需要进一步研究。未来的研究还应探讨γδ T细胞活化和il -21介导的免疫是否与寄生虫控制机制有关。

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引用次数: 0

Shannon entropy as a stable diffusion tensor parameter for evaluating normal spinal cord regions in dogs Shannon熵作为一种稳定的扩散张量参数用于评价狗的正常脊髓区域

IF 1.8 3区农林科学 Q1 VETERINARY SCIENCES

Research in veterinary science

Pub Date : 2026-03-01 Epub Date: 2025-12-18 DOI: 10.1016/j.rvsc.2025.106036

Yoshimichi Goda , Yuko Mizutani , Mizuki Hirayama , Taketoshi Asanuma , Hiroyuki Satoh

Diffusion tensor imaging (DTI) has been used in veterinary medicine for cerebral infarctions and degenerative diseases in recent years. Defining normal values for the apparent diffusion coefficient (ADC) and fractional anisotropy (FA) to detect lesions is challenging because these values depend on anatomical differences in the spinal cord, magnetic resonance imaging (MRI) machine model, magnetic field strength, and measurement protocol. Shannon entropy is the average amount of information and is a measure of the variability or complexity, of distributed numbers. The purpose of this study is to evaluate whether Shannon entropy can serve as a useful new indicator for interpreting the structural integrity of the canine spinal cord. We used six beagle dogs with no spinal cord disease, as confirmed by neurological examination and magnetic resonance imaging. We calculated the respective values for the cervical and thoracolumbar spinal cord under the 3 T magnetic field. The ADC and FA values (mean ± standard error) of the cervical spinal cord were 1.11 ± 0.05 × 10⁻³ mm²/s and 0.64 ± 0.02. The thoracolumbar spinal cord had values of 1.16 ± 0.04 × 10⁻³ mm²/s and 0.58 ± 0.02. The ADC and FA values of the spinal cord reflected anatomical differences, and no fixed normal values were observed for the entire spinal cord. Shannon entropy was 5.67 ± 0.45. No significant differences were observed in the values at any intervertebral level. These results suggest that Shannon entropy is more useful than the ADC and FA values. Shannon entropy may be able to differentiate between normal tissue and lesions, independent of various MRI conditions.

近年来，弥散张量成像（DTI）在兽医学中用于脑梗死和退行性疾病的诊断。定义表观扩散系数（ADC）和分数各向异性（FA）的正常值以检测病变是具有挑战性的，因为这些值取决于脊髓的解剖差异、磁共振成像（MRI）机器模型、磁场强度和测量方案。香农熵是信息的平均量，是对分布式数字的可变性或复杂性的度量。本研究的目的是评估香农熵是否可以作为一个有用的新指标来解释犬脊髓的结构完整性。我们使用6只没有脊髓疾病的比格犬，经神经学检查和磁共振成像证实。我们计算了在3t磁场下颈脊髓和胸腰椎的相应值。颈脊髓的ADC和FA值（平均±标准误差）分别为1.11±0.05 × 10−3 mm2/s和0.64±0.02。胸腰段脊髓分别为1.16±0.04 × 10−3 mm2/s和0.58±0.02。脊髓的ADC和FA值反映了解剖上的差异，整个脊髓没有固定的正常值。Shannon熵为5.67±0.45。在任何椎间水平的数值均未观察到显著差异。这些结果表明香农熵比ADC和FA值更有用。香农熵可能能够区分正常组织和病变，独立于各种MRI条件。

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引用次数: 0

Quantitative evaluation and multivariate analysis-based ranking of factors influencing blood proviral load in breeding cattle infected with bovine leukemia virus 牛白血病病毒感染种牛血液原病毒载量影响因素的定量评价和多变量分析排序。

IF 1.8 3区农林科学 Q1 VETERINARY SCIENCES

Research in veterinary science

Pub Date : 2026-02-01 Epub Date: 2025-11-30 DOI: 10.1016/j.rvsc.2025.105995

Yuki Fujii , Masaki Maezawa , Shun Ishizuka , Yuki Ishida , Shoko Ooya , Yuki Kashima , Kaoru Yamashita , Nahoko Kawanishi , Masataka Akagami , Yoshiko Ootani , Akiyuki Watanabe , Satoshi Takahashi , Masato Ishii , Junko Kawakami , Yuri Fujimoto , Hisashi Inokuma

Bovine leukemia virus (BLV) infection in cattle results in variable proviral load (PVL), a key indicator of viral transmission and disease progression. However, the relative contributions of host and viral factors to PVL are not fully understood. The present study evaluated four potential determinants of PVL in BLV-infected cattle: two host-related factors (BoLA-DRB3 alleles and age) and two viral factors (Tax amino acid 233 (TAX-233) and 5’LTR nucleotide 175 (LTR-175)) in 1673 Japanese Black (JB) and 979 Holstein Friesian (HF) breeding cows. The contribution of each factor to PVL was quantified using Quantification Theory Type I. BoLA-DRB3 and age accounted for the greatest variation in both JB (55.1 % and 32.4 %) and HF (65.6 % and 18.2 %) cattle, while TAX-233 and LTR-175 made smaller contributions. Notably, the L233 and 175C variants—linked to increased tumorigenicity and transmissibility—were significantly associated with higher PVL in HF but not JB cattle, suggesting breed-specific viral effects. Despite their clinical relevance, these viral markers explained PVL variation to a lesser degree than host factors. These findings underscore the limitations of PVL-based culling strategies, since low-PVL animals may still harbor high-risk viral genotypes. We recommend the integration of viral genotyping into risk assessment frameworks to improve control measures. Although our multivariable models explained only a modest portion of PVL variance (R² = 5.5–11.7 %), the independent contributions of each factor were confirmed. Future studies incorporating additional host, viral, and environmental variables will be essential to refine predictive models for BLV pathogenesis and transmission.

牛白血病病毒（BLV）感染导致可变前病毒载量（PVL），这是病毒传播和疾病进展的关键指标。然而，宿主和病毒因子对PVL的相对贡献尚未完全了解。本研究在1673头日本黑牛（JB）和979头荷斯坦黑牛（HF）中评估了blv感染牛PVL的四个潜在决定因素：两个宿主相关因素（BoLA-DRB3等位基因和年龄）和两个病毒因素（Tax氨基酸233 （Tax -233）和5'LTR核苷酸175 (LTR-175)）。各因子对PVL的贡献采用量化理论类型1进行了量化。BoLA-DRB3和年龄对JB牛（55.1%和32.4%）和HF牛（65.6%和18.2%）的贡献最大，而TAX-233和LTR-175的贡献较小。值得注意的是，与增加的致瘤性和传播性相关的L233和175C变异与HF牛的PVL升高显著相关，而与JB牛的PVL升高无关，这表明了品种特异性病毒效应。尽管它们具有临床相关性，但这些病毒标记物对PVL变异的解释程度低于宿主因素。这些发现强调了基于pvl的扑杀策略的局限性，因为低pvl的动物可能仍然携带高风险的病毒基因型。我们建议将病毒基因分型纳入风险评估框架，以改进控制措施。虽然我们的多变量模型只解释了PVL方差的一小部分（R2 = 5.5- 11.7%），但每个因素的独立贡献都得到了证实。纳入其他宿主、病毒和环境变量的未来研究对于完善BLV发病机制和传播的预测模型至关重要。

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引用次数: 0

Spatiotemporal distribution and genetic characteristics of porcine reproductive and respiratory syndrome virus-2 lineage 1 in Japan from 2020 to 2023 2020 - 2023年日本猪繁殖与呼吸综合征病毒2谱系1的时空分布及遗传特征

IF 1.8 3区农林科学 Q1 VETERINARY SCIENCES

Research in veterinary science

Pub Date : 2026-02-01 Epub Date: 2025-12-13 DOI: 10.1016/j.rvsc.2025.106025

Ryosuke Takai , Tetsuo Sato , Yoriko Yonezawa , Nobuyuki Tsutsumi , Ryohei Nukui , Katsuaki Sugiura , Osamu Taira

Porcine reproductive and respiratory syndrome (PRRS), caused by the PRRS virus (PRRSV), is a major swine disease globally. This study analyzed 1207 PRRSV sequences (2020-2023) detected through routine diagnostic submissions in Japan using the standardized open reading frame 5-based lineage classification system. Phylogenetic analysis identified six lineages in Japan: L1, L4, L5, L8, L9, and L11. Notably, the prevalence of L1 showed a marked increase during the study period. L1 strains were predominantly detected in the Kanto and Kyushu regions. In Kanto, L1B was predominant (80 %), whereas Kyushu exhibited greater sublineage diversity (L1A, L1B, L1E, and L1I). Pairwise nucleotide identity analysis revealed high genetic heterogeneity within the L1 population (mean identity 90.1 %), while L5 and L8 populations were highly homogenous (97.9 % and 98.3 %, respectively) and showed high sequence identity (>95 %) to commercial vaccine strains. This study presents the first nationwide molecular epidemiological analysis of PRRSV in Japan based on the global lineage system. The findings highlight Japan's distinct L1 sublineage dynamics (e.g., L1B dominance) compared to global trends (e.g., L1C.5 in the US and NADC30/34-like strains in China and Korea), offering critical insights for informing effective PRRSV control strategies.

猪繁殖与呼吸综合征（PRRS）是由猪繁殖与呼吸综合征病毒（PRRSV）引起的一种全球性的主要猪疾病。本研究使用基于标准化开放阅读框5的谱系分类系统，分析了通过日本常规诊断提交检测到的1207个PRRSV序列（2020-2023）。系统发育分析在日本鉴定出6个谱系：L1、L4、L5、L8、L9和L11。值得注意的是，在研究期间，L1的患病率明显增加。L1菌株主要在关东和九州地区检出。关东地区以L1B亚系为主（80%），九州地区则表现出较大的亚系多样性（L1A、L1B、L1E和L1I）。两两核苷酸同源性分析显示，L1群体具有较高的遗传异质性（平均同源性为90.1%），而L5和L8群体具有较高的同源性（分别为97.9%和98.3%），与商业疫苗株具有较高的序列同源性（> 95%）。本研究首次基于全球谱系系统对日本PRRSV进行了全国范围的分子流行病学分析。与全球趋势（如L1C）相比，研究结果突出了日本独特的L1亚谱系动态（如L1B主导）。5在美国，nadc30 /34样毒株在中国和韩国)，为通报有效的PRRSV控制策略提供了关键见解。

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引用次数: 0

Effects of dietary supplementation of an oleuropein-enriched olive leaf extract on performance, carcass characteristics, intestinal health, foot pad parameters and meat quality of broilers 饲粮中添加富含橄榄苦苷的橄榄叶提取物对肉鸡生产性能、胴体特性、肠道健康、脚垫参数和肉品质的影响

IF 1.8 3区农林科学 Q1 VETERINARY SCIENCES

Research in veterinary science

Pub Date : 2026-02-01 Epub Date: 2025-11-25 DOI: 10.1016/j.rvsc.2025.105990

Konstantina Vasilopoulou , Georgios A. Papadopoulos , Styliani Lioliopoulou , Leandros Skaltsounis , Panagiotis Stathopoulos , Ioanna Stylianaki , Dimitrios Galamatis , Vasileios Tsiouris , Georgios Arsenos , Ilias Giannenas

Incorporating agricultural by-products into animal diets represents a sustainable strategy to enhance animal health and product quality. Olive leaves, rich in bioactive compounds such as oleuropein, exhibit notable antioxidant, antimicrobial, and anti-inflammatory properties. This study evaluated the effects of dietary supplementation with an environmentally friendly, oleuropein-enriched OLE (OLE) on broiler chickens. A total of 480 day-old chicks were randomly assigned to four groups: a control diet - CF (T1), CF + 1 % OLE (T2), CF + 2.5 % OLE (T3), and CF + 0.1 % encapsulated oregano oil (T4, positive control). Over 42 days, growth performance, carcass characteristics, intestinal morphology, foot pad health, and meat quality were assessed. While T4 showed superior growth performance, the 1 % OLE diet significantly improved intestinal morphology and meat quality. A dose-dependent yellow pigmentation of the foot pad was observed in OLE-treated groups. Additionally, breast tenderness and darker thigh coloration improved in OLE-fed broilers. These findings suggest that 1 % OLE can be a viable natural additive to support gut health and meat quality in broilers, with comparable antioxidant effects to oregano oil and no adverse outcomes.

将农业副产品纳入动物饲料是提高动物健康和产品质量的可持续战略。橄榄叶富含橄榄苦苷等生物活性化合物，具有显著的抗氧化、抗菌和抗炎特性。本研究评价了饲粮中添加一种富含橄榄苦苷的环保型OLE （OLE）对肉鸡的影响。选取480日龄雏鸡，随机分为4组：对照饲粮- CF （T1）、CF + 1% OLE （T2）、CF + 2.5% OLE （T3）和CF + 0.1%胶囊牛至油（T4，阳性对照）。在42 d内，对生长性能、胴体特性、肠道形态、脚垫健康和肉品质进行评估。虽然T4表现出优异的生长性能，但1% OLE日粮显著改善了肠道形态和肉品质。在ole处理组中观察到足垫的剂量依赖性黄色色素沉着。此外，ole饲养肉鸡的乳房柔软度和大腿颜色较深有所改善。这些研究结果表明，1% OLE是一种可行的天然添加剂，可支持肉鸡肠道健康和肉品质，具有与牛至油相当的抗氧化效果，且无不良后果。

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引用次数: 0

Pilot study: Effects of whole-body vibrational therapy on equine thoracolumbar epaxial muscle response to pressure algometry 初步研究：全身振动疗法对马胸腰椎外轴肌对压力测量反应的影响。

IF 1.8 3区农林科学 Q1 VETERINARY SCIENCES

Research in veterinary science

Pub Date : 2026-02-01 Epub Date: 2025-12-15 DOI: 10.1016/j.rvsc.2025.106027

Riley Leibeck , Jeffrey Lehman , Sheri Birmingham

Background

Whole-body vibrational therapy (WBV) is well-studied in people, but there is a paucity of research regarding its effect on horses. It is suggested that the use of WBV in horses increases performance, aids in recovery, and stimulates nerves, bones, and muscles in ways that normal exercise does not.

Objective

The purpose of this pilot study was to determine if frequent WBV therapy sessions affect mechanical nociceptive thresholds based on pressure algometry in horses without clinical signs of lameness or back pain.

Methods

The mechanical nociceptive thresholds (MNTs) were measured for 5 horses at 8 points along their thoracolumbar epaxial muscles once every 7 days for 8 weeks. Baseline MNT scores were established during a control period with no vibration therapy treatment over the first 4 weeks. During the second 4-week period, horses received 5, 30-min vibration therapy sessions per week.

Results

Despite previous research indicating that WBV increases epaxial muscle symmetry in horses, no significant difference was noted between the horses' mean MNTs across all 8 sites during the baseline and treatment periods. Upon further investigation of the change in sensitivity at the individual sites across all subjects, a statistically significant (p < 0.05) increase in MNT (0.49–0.62 kgf) was observed at 4 out of the 8 sites.

Conclusion

While an increase in mean MNTs was not observed, this study found that WBV may increase MNTs at individual sites along the thoracolumbar epaxial musculature in horses. Further investigation is required to assess whether this difference is biologically meaningful and repeatable.

背景：全身振动疗法（WBV）在人身上得到了很好的研究，但关于其对马的影响的研究却很少。这表明，在马身上使用WBV可以提高表现，帮助恢复，并刺激神经，骨骼和肌肉，这是正常运动所不能达到的。目的：本初步研究的目的是确定频繁的WBV治疗是否会影响无跛行或背痛临床症状的马的机械伤害感受阈值。方法：测定5匹马胸腰段外轴肌8个点的机械伤害知觉阈值（mnt），每7天1次，连续8周。基线MNT评分是在前4周没有振动治疗的对照期内建立的。在第二个4周期间，马每周接受5次30分钟的振动治疗。结果：尽管先前的研究表明，WBV增加了马的外轴肌对称性，但在基线和治疗期间，马在所有8个部位的平均mnt之间没有显着差异。结论：虽然没有观察到平均MNTs的增加，但本研究发现，WBV可能会增加马胸腰椎外轴肌组织个别部位的MNTs。需要进一步的调查来评估这种差异是否具有生物学意义和可重复性。

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引用次数: 0

Strategic use of enzyme-linked immunosorbent assay as a complementary tool for the diagnosis of bovine tuberculosis 战略性地使用酶联免疫吸附试验作为牛结核病诊断的补充工具。

IF 1.8 3区农林科学 Q1 VETERINARY SCIENCES

Research in veterinary science

Pub Date : 2026-02-01 Epub Date: 2025-12-04 DOI: 10.1016/j.rvsc.2025.106017

C. Camussone , R.D. Moyano , M.N. Alonso , J. Cicotello , A. Peña , A.I. Molineri , D.M. Astesana , G. Suárez-Archilla , F. Tello D'Elia , D. Aliprandi , N.M. Welschen , M.L. Signorini , M.V. Zbrun

Bovine tuberculosis (bTB) is a global chronic disease. Argentina's bTB eradication program consists of the Caudal-Fold Tuberculin test (CFT) and the sacrifice of positive animals as the principal measure. However, despite the implementation of the control program, CFT-positive animals continue to appear in some herds, which generates skepticism about the potential to eradicate tuberculosis. The objective of this work was to evaluate the suitability of an ELISA test for the detection of M. bovis specific antibodies (bTB-ELISA) in CFT-negative animals as a complementary strategy to control bTB. A convenience sampling of dairy farms was conducted through contact with owners, and those who agreed to the research protocol were selected. Two studies were conducted in eight (study 1) and six (study 2) dairy farms. Cows were tested by CFT, and those negative to this test were tested using ELISA in three cycles, one cycle per year during three consecutive years (study 1) and three cycles applied consecutively for 1 year (study 2). The prevalence of bTB-ELISA-positive animals showed a significant reduction towards the third sampling in both strategies. Although the general trend was towards a reduction in bTB-positive animals using CFT, this behavior was not homogeneous among dairy farms, evidencing that intra-farm factors are relevant. The repeated application of the CFT test together with ELISA was a useful strategy for the reduction of CFT-positive animals. It should be noted that this approach must be accompanied by good management practices in the herd to prevent the entry and spread of the disease.

牛结核病（bTB）是一种全球性的慢性病。阿根廷的bTB根除规划包括尾褶结核菌素试验（CFT）和作为主要措施的阳性动物的牺牲。然而，尽管实施了控制规划，一些畜群中仍出现cft阳性动物，这使人们对根除结核病的潜力产生怀疑。本研究的目的是评估ELISA检测牛分枝杆菌特异性抗体（bTB-ELISA）在cft阴性动物中作为控制bTB的补充策略的适用性。通过与奶牛场所有者的联系，对奶牛场进行了方便的抽样，并选择了同意研究方案的奶牛场。两项研究分别在8个（研究1）和6个（研究2）奶牛场进行。采用CFT法对奶牛进行检测，检测阴性奶牛采用ELISA法分3个周期进行检测，连续3年每年1个周期（研究1），连续1年3个周期（研究2）。在这两种策略中，btb - elisa阳性动物的患病率在第三次采样时均显着降低。尽管使用CFT的总体趋势是减少结核杆菌阳性动物，但这种行为在奶牛场之间并不均匀，这表明农场内部因素是相关的。重复应用CFT测试和ELISA是减少CFT阳性动物的有效策略。应该指出的是，这种方法必须伴随着良好的牛群管理做法，以防止疾病的进入和传播。

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引用次数: 0

Growth, physiological responses, and histopathological changes of Oreochromis niloticus fingerlings exposed to aqueous gold nanoparticles (AuNs) at high stocking density 高放养密度条件下尼罗鱼（Oreochromis niloticus）鱼种生长、生理反应及组织病理学变化

IF 1.8 3区农林科学 Q1 VETERINARY SCIENCES

Research in veterinary science

Pub Date : 2026-02-01 Epub Date: 2025-11-26 DOI: 10.1016/j.rvsc.2025.105991

Randa Y. Thabet , Sameh M. Farouk , Shahad M. Elkim , Ashraf Y. El-Dakar , Sherifa H. Ahmed , Dalia S. Hamza , Omar Abdel-hamed Ahmed-Farid , Mohamed F. Abdel-Aziz

Despite the ubiquitous usage of nanoparticles (Ns), there are still significant gaps of knowledge regarding the impact of gold nanoparticles (AuNs) on one of the economically important fish species as Nile tilapia (Oreochromis niloticus). Therefore, this study was conducted to investigate the impact of gold nanoparticles (AuNs) on the growth performance, serum biochemistry, antioxidant enzymes levels, digestive enzyme activity, and histopathological changes of different studied tissues such as gills and liver in Nile tilapia (Oreochromis niloticus) that exposed to various concentrations of AuNs (0, 5, 10, 15 and 20 μgL⁻¹) respectively as aqueous exposure, particularly in the context of heavy metal exposure. Fish were randomly distributed in 15 glass aquariums (70 L) at a stocking rate of 20 fish/aquarium (treatment = 3 aquariums: 60 fish). Fish were within an initial average weight of 4.02 g ± 0.27 and fed a commercial diet containing 30 % crude protein (CP) at a feeding rate of 4 % of biomass. Results showed improvements in growth with AuNs exposure, but the survival rate decreased with increasing concentrations of AuNs exposure. The 20 μg L⁻¹ AuNs group exhibited the highest specific growth rate (SGR: 3.45 %/day), representing a 17.7 %/day increase compared to the control (2.93 %/day), alongside a 44.7 % reduction in survival rate (52.55 % vs. 95 % in controls). Intermediate concentrations (e.g., 5 or 10 μg L-1 AuNs) improved SGR (3.16 and 3.23 %/day) with less severe survival impacts (92.5 and 75 %, respectively).

Also, increasing doses of AuNs led to negative effects on physiological status and histological study of the liver and gills. Fish that were exposed to ≤5 μg L⁻¹ exhibited the highest survival rate and enhanced resistance to heavy-metal challenge. It can be recommended that aqueous exposure to gold nanoparticles requires further studies, and it appears that doses less than 5 μm may have positive effects on growth and immune functions.

尽管纳米颗粒（Ns）的广泛使用，但关于金纳米颗粒（AuNs）对尼罗罗非鱼（Oreochromis niloticus）这一重要经济鱼类的影响，人们仍然存在重大的知识空白。因此，本研究旨在研究金纳米粒子（AuNs）对尼罗罗非鱼（Oreochromis niloticus）生长性能、血清生化、抗氧化酶水平、消化酶活性以及不同组织（如鳃和肝脏）的影响，这些组织分别暴露于不同浓度的AuNs（分别为0、5、10、15和20 μgL−1）中，特别是在重金属暴露的背景下。鱼随机分布在15个玻璃水族箱（70 L）中，放养率为20只/个水族箱（处理= 3个水族箱：60只）。试验鱼初始平均体重为4.02 g±0.27，投喂含30%粗蛋白质（CP）的商品饲料，投喂率为生物量的4%。结果显示，AuNs暴露改善了生长，但存活率随着AuNs暴露浓度的增加而下降。20 μg L−1 AuNs组的特定生长率最高（SGR: 3.45% /天），比对照组（2.93% /天）提高了17.7% /天，而存活率降低了44.7%（52.55%比95%）。中等浓度（例如，5或10 μg L-1 AuNs）提高了SGR（3.16%和3.23% /天），对生存的影响较轻（分别为92.5%和75%）。此外，增加AuNs的剂量会对肝脏和鳃的生理状态和组织学研究产生负面影响。当浓度≤5 μg L−1时，鱼的存活率最高，对重金属的抗性增强。可以建议，在水中暴露于金纳米颗粒需要进一步的研究，似乎剂量小于5 μm可能对生长和免疫功能有积极影响。

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引用次数: 0

Mechanical characterization of the Robert Jones bandage under cyclic compression-bending: An in vitro experimental model 罗伯特·琼斯绷带在循环压缩弯曲下的力学特性：体外实验模型。

IF 1.8 3区农林科学 Q1 VETERINARY SCIENCES

Research in veterinary science

Pub Date : 2026-02-01 Epub Date: 2025-12-06 DOI: 10.1016/j.rvsc.2025.106018

Samuel Larcheveque , Stéphanie Claeys , Marc Balligand , Pierre P. Picavet

Objective

To assess the rigidity of Robert-Jones Bandage (RJB) used to stabilize a standardized in vitro fracture model, under cyclic compression/bending.

Study design

In vitro cyclic testing study. Specimens: wooden rods divided into two segments of identical length by a Cardan Joint enclosed in a thick foam tube and supported by a RJB. Procedures: Part 1: incremental loading applied to 6 specimens in successive stages of 50 N from 5 to 150 to 5–300 N with 3000 cycles per stage. Part 2: 9 specimens were loaded at 150 N (5–150 N) during 60,000 cycles.

Statistics

The Wilcoxon signed-rank test was used to compare data obtained at 10,000 and 60,000 cycles.

Results

In part 1, 6/6 specimens withstood 150 N for 3000 cycles. Three specimens endured an additional 3000 cycles at 200 N, 2 withstood 250 N for another 3000 cycles, and 1 tolerated 300 N. In part 2, 9/9 specimens withstood 150 N for 60,000 cycles, with displacements ranging from 0.102 mm to 0.309 mm (mean 0.179 ± 0.069 mm) and a median rigidity of 897 ± 375 N/mm. No specimen reached the fixed displacement limit of 2 mm after 60,000 cycles. No statistically significant difference was found between the results at 10,000 and 60,000 cycles (p = 0.129).

Conclusion

The Robert-Jones Bandage showed consistent rigidity under prolonged cyclic loading in an in vitro fracture model.

Clinical relevance

While this in-vitro model cannot replicate the influence of soft tissues, joint motion, or multidirectional physiological forces, the findings suggest that a properly applied Robert Jones bandage may provide short-term mechanical support to a long-bone fracture prior to definitive stabilization. However, clinical applicability should be interpreted with caution given the inherent limitations of the experimental setup.

目的：评估罗伯特-琼斯绷带（RJB）在循环压缩/弯曲下用于稳定标准化体外骨折模型的刚度。研究设计：体外循环试验研究。样品：用粗泡沫管包裹的万向接头将木棒分成两段，长度相同，由RJB支撑。程序：第1部分：在5- 150 - 5-300牛的50牛的连续阶段中对6个试样施加增量加载，每阶段3000次循环。第2部分：9个试样在150n （5- 150n）下加载60000次。统计学：使用Wilcoxon符号秩检验比较10,000和60,000次循环的数据。结果：在第1部分中，6/6的标本承受了150 N的3000次循环。在第2部分中，9/9试件在150 N下承受了60,000次循环，位移范围从0.102 mm到0.309 mm（平均0.179±0.069 mm），中位刚度为897±375 N/mm。6万次循环后，没有试件达到2 mm的固定位移极限。1万次和6万次循环的结果无统计学差异（p = 0.129）。结论：在体外骨折模型中，罗伯特-琼斯绷带在长时间循环载荷下表现出一致的刚性。临床意义：虽然该体外模型不能复制软组织、关节运动或多向生理力的影响，但研究结果表明，在最终稳定之前，适当使用Robert Jones绷带可以为长骨骨折提供短期机械支持。然而，鉴于实验设置的固有局限性，临床适用性应谨慎解释。

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引用次数: 0