Abstract Sensitive DNA assays are of importance in life science and biomedical engineering, but they are heavily dependent on thermal cycling programs or enzyme-assisted schemes, which require the utilization of bulky devices and costly reagents. To circumvent such requirements, we developed an isothermal enzyme-free DNA sensing method with dual-stage signal amplification ability based on the coupling use of catalytic hairpin assembly (CHA) and Mg2+-dependent deoxyribozyme (DNAzyme). In this study, the sensing system involves a set of hairpin DNA probes for CHA (ensuring the first stage of signal amplification) as well as ribonucleobase-modified molecular beacons that serve as activatable substrates for DNAzymes (warranting the second stage of signal amplification). An experimentally determined detection limit of about 0.5 pM is achieved with a good linear range from 0.5 to 10 pM. The results from spiked fetal bovine serum samples further confirm the reliability for practical applications. The non-thermal cycling, enzyme-free, and dual-amplified features make it a powerful sensing tool for effective nucleic acid assay in a variety of biomedical applications.
{"title":"An isothermal, non-enzymatic, and dual-amplified fluorescent sensor for highly sensitive DNA detection","authors":"I. Iwe, Zhigang Li","doi":"10.1515/revac-2021-0140","DOIUrl":"https://doi.org/10.1515/revac-2021-0140","url":null,"abstract":"Abstract Sensitive DNA assays are of importance in life science and biomedical engineering, but they are heavily dependent on thermal cycling programs or enzyme-assisted schemes, which require the utilization of bulky devices and costly reagents. To circumvent such requirements, we developed an isothermal enzyme-free DNA sensing method with dual-stage signal amplification ability based on the coupling use of catalytic hairpin assembly (CHA) and Mg2+-dependent deoxyribozyme (DNAzyme). In this study, the sensing system involves a set of hairpin DNA probes for CHA (ensuring the first stage of signal amplification) as well as ribonucleobase-modified molecular beacons that serve as activatable substrates for DNAzymes (warranting the second stage of signal amplification). An experimentally determined detection limit of about 0.5 pM is achieved with a good linear range from 0.5 to 10 pM. The results from spiked fetal bovine serum samples further confirm the reliability for practical applications. The non-thermal cycling, enzyme-free, and dual-amplified features make it a powerful sensing tool for effective nucleic acid assay in a variety of biomedical applications.","PeriodicalId":21090,"journal":{"name":"Reviews in Analytical Chemistry","volume":"30 1","pages":"312 - 322"},"PeriodicalIF":4.3,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77714105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract Even very low concentrations of heavy metal pollutants have adverse effects on the environment and on human health. Thus, determining even trace concentrations of heavy metals in various samples has attracted a lot of attention. The conventional analytical methods used for the sampling and analysis of heavy metals have some limitations, including the effects of the matrix and their high detection limits. Thus, various methods are used for the pretreatment and concentration of the target analytes, and these methods are time-consuming, expensive, and require the use of toxic solvents. In recent years, supramolecular solvent-based microextraction (SSME), a green analytical strategy, has been used to determine low concentrations of heavy metals in various matrices. This method has unique features such as high enrichment factor, short extraction time, and rapid analysis. In addition, it is cost effective because it consumes less chemical reagents than other methods. Also, it is ecofriendly, and it has good sensitivity and selectivity. Herein, we presented a comprehensive review of the application of the SSME technique for the analysis of heavy metals in water, food, and biological samples. Also, we have provided the distinctive properties of the SSME technique, discussed the challenges that lie ahead, and addressed the potential future trend.
{"title":"Supramolecular solvent-based microextraction techniques for sampling and preconcentration of heavy metals: A review","authors":"V. Jalili, R. Zendehdel, A. Barkhordari","doi":"10.1515/revac-2021-0130","DOIUrl":"https://doi.org/10.1515/revac-2021-0130","url":null,"abstract":"Abstract Even very low concentrations of heavy metal pollutants have adverse effects on the environment and on human health. Thus, determining even trace concentrations of heavy metals in various samples has attracted a lot of attention. The conventional analytical methods used for the sampling and analysis of heavy metals have some limitations, including the effects of the matrix and their high detection limits. Thus, various methods are used for the pretreatment and concentration of the target analytes, and these methods are time-consuming, expensive, and require the use of toxic solvents. In recent years, supramolecular solvent-based microextraction (SSME), a green analytical strategy, has been used to determine low concentrations of heavy metals in various matrices. This method has unique features such as high enrichment factor, short extraction time, and rapid analysis. In addition, it is cost effective because it consumes less chemical reagents than other methods. Also, it is ecofriendly, and it has good sensitivity and selectivity. Herein, we presented a comprehensive review of the application of the SSME technique for the analysis of heavy metals in water, food, and biological samples. Also, we have provided the distinctive properties of the SSME technique, discussed the challenges that lie ahead, and addressed the potential future trend.","PeriodicalId":21090,"journal":{"name":"Reviews in Analytical Chemistry","volume":"117 3","pages":"93 - 107"},"PeriodicalIF":4.3,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/revac-2021-0130","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72479686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
K. Elgendy, M. A. Elmosallamy, M. K. Soltan, A. Amin, D. S. Elshaprawy
Abstract Two new potentiometric sensors were created for the quantification of bisoprolol fumarate and alverine citrate in bulk pharmaceutical dosage forms and human serum. Bisoprolol and alverine sensors were manufactured by combining potassium tetrakis (p-chlorophenyl) borate ion pairs to serve as electroactive substances, plasticized poly (vinyl chloride) matrix membranes, and o-nitrophenyl octyl ether. They demonstrated high responses over the concentration ranges of 1.0×10−6 to 1.0×10−2 mol L−1 bisoprolol and alverine with close to Nernstian cationic slopes of 52 and 56 mV decade−1, respectively. The detection limits for bisoprolol and alverine were 2.6×10−6 and 1.75×10−6 mol L−1, respectively. For both medications, the response time was instantaneous (2.0 s). The working pH ranges for bisoprolol and alverine were 4.50–8.50 and 2.00–8.80, respectively. For both sensors, the life cycle was long (3 months). The sensors were used in pharmaceutical dosage types for the assay of bisoprolol and alverine, recording average recoveries of 99.40% and 99.98% respectively and were also successfully used for estimating the two drugs in human serum with an average recovery of 99.60% for both drugs. For all multiple staged interfering materials, the reported latest potentiometric sensor methods displayed high selectivity. The current sensor obtained a high percentage recovery and an excellent relative standard deviation compared with those obtained from previously published methods.
{"title":"Novel potentiometric methods for the estimation of bisoprolol and alverine in pharmaceutical forms and human serum","authors":"K. Elgendy, M. A. Elmosallamy, M. K. Soltan, A. Amin, D. S. Elshaprawy","doi":"10.1515/revac-2021-0129","DOIUrl":"https://doi.org/10.1515/revac-2021-0129","url":null,"abstract":"Abstract Two new potentiometric sensors were created for the quantification of bisoprolol fumarate and alverine citrate in bulk pharmaceutical dosage forms and human serum. Bisoprolol and alverine sensors were manufactured by combining potassium tetrakis (p-chlorophenyl) borate ion pairs to serve as electroactive substances, plasticized poly (vinyl chloride) matrix membranes, and o-nitrophenyl octyl ether. They demonstrated high responses over the concentration ranges of 1.0×10−6 to 1.0×10−2 mol L−1 bisoprolol and alverine with close to Nernstian cationic slopes of 52 and 56 mV decade−1, respectively. The detection limits for bisoprolol and alverine were 2.6×10−6 and 1.75×10−6 mol L−1, respectively. For both medications, the response time was instantaneous (2.0 s). The working pH ranges for bisoprolol and alverine were 4.50–8.50 and 2.00–8.80, respectively. For both sensors, the life cycle was long (3 months). The sensors were used in pharmaceutical dosage types for the assay of bisoprolol and alverine, recording average recoveries of 99.40% and 99.98% respectively and were also successfully used for estimating the two drugs in human serum with an average recovery of 99.60% for both drugs. For all multiple staged interfering materials, the reported latest potentiometric sensor methods displayed high selectivity. The current sensor obtained a high percentage recovery and an excellent relative standard deviation compared with those obtained from previously published methods.","PeriodicalId":21090,"journal":{"name":"Reviews in Analytical Chemistry","volume":"43 1","pages":"127 - 135"},"PeriodicalIF":4.3,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82080106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract Fast pyrolysis of lignocellulosic biomass is one of the most promising methods of the production of renewable fuels. However, an optimization of the conditions of bio-oil production is not possible without comprehensive analysis of the composition of formed products. There are several methods for the determination of distribution of products formed during thermal decomposition of biomass with chromatography being the most versatile among them. Although, due to the complex structure of bio-oil (presence of hundreds chemical compounds with different chemical character), an interpretation of the obtained chromatograms is not an easy task. Therefore, the aim of this work is to present an application of different chromatographic methods to the analysis of the composition of the mixture of products formed in high temperature decomposition of lignocellulosic feedstock. It includes pyrolysis-gas chromatography/mass spectrometry (Py-GC/MS), two dimensional gas (GC x GC) or liquid chromatography (LC x LC) and initial fractionation of bio-oil components. Moreover, the problems connected with the analysis of bio-oils formed with the use of various fast pyrolysis reactors and capabilities of multivariate analysis are discussed.
木质纤维素生物质的快速热解是生产可再生燃料最有前途的方法之一。然而，如果没有对形成产物的成分进行全面分析，就不可能优化生物油的生产条件。测定生物质热分解过程中形成的产物分布的方法有几种，其中色谱法是最通用的。虽然，由于生物油的复杂结构(存在数百种具有不同化学性质的化合物)，获得的色谱图的解释不是一件容易的事情。因此，这项工作的目的是介绍不同色谱方法的应用，以分析高温分解木质纤维素原料形成的混合物的组成。它包括热解-气相色谱/质谱(Py-GC/MS)，二维气相色谱(GC x GC)或液相色谱(LC x LC)和生物油成分的初始分馏。此外，还讨论了使用各种快速热解反应器对生物油进行分析所涉及的问题以及多元分析的能力。
{"title":"Chromatographic analysis of bio-oil formed in fast pyrolysis of lignocellulosic biomass","authors":"J. Grams","doi":"10.1515/revac-2020-0108","DOIUrl":"https://doi.org/10.1515/revac-2020-0108","url":null,"abstract":"Abstract Fast pyrolysis of lignocellulosic biomass is one of the most promising methods of the production of renewable fuels. However, an optimization of the conditions of bio-oil production is not possible without comprehensive analysis of the composition of formed products. There are several methods for the determination of distribution of products formed during thermal decomposition of biomass with chromatography being the most versatile among them. Although, due to the complex structure of bio-oil (presence of hundreds chemical compounds with different chemical character), an interpretation of the obtained chromatograms is not an easy task. Therefore, the aim of this work is to present an application of different chromatographic methods to the analysis of the composition of the mixture of products formed in high temperature decomposition of lignocellulosic feedstock. It includes pyrolysis-gas chromatography/mass spectrometry (Py-GC/MS), two dimensional gas (GC x GC) or liquid chromatography (LC x LC) and initial fractionation of bio-oil components. Moreover, the problems connected with the analysis of bio-oils formed with the use of various fast pyrolysis reactors and capabilities of multivariate analysis are discussed.","PeriodicalId":21090,"journal":{"name":"Reviews in Analytical Chemistry","volume":"43 1","pages":"65 - 77"},"PeriodicalIF":4.3,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79485272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Paulina Gątarek, Jagoda Jóźwik-Pruska, Geir Bjorklund, S. Chirumbolo, J. Kałużna-Czaplińska
Abstract In this study, the levels of concentration of carboxylic acids (benzoic acid, p-hydroxybenzoic acid, p-hydroxyphenylacetic acid, and hippuric acid) in the urine of autistic children were investigated and compared. The increased excretion of carboxylic acids is related to excessive bacterial activity in the gut, called bacterial overgrowth, which has been related to autism spectrum disorder (ASD) as an impairment in the gut-brain axis. The investigation was based on the analysis of urine samples obtained from 120 ASD children. To identify and quantify urinary carboxylic acids (UCAs), we applied gas chromatography coupled with mass spectrometry (GC-MS). Additionally, we checked the influence of probiotic supplementation, gender, body mass index (BMI) value and age of children on the level of different selected compounds. Most of the obtained results were found within reference ranges. In some cases, the levels of benzoic acid, p-hydroxybenzoic acid, and p-hydroxyphenylacetic acid were particularly elevated. Statistically significant differences were observed in supplementation with probiotics and the level of p-hydroxyphenylacetic acid (p=0.036). The obtained results may indicate disturbances in the intestinal flora in some autistic patients and suggest that supplements may have an influence on the levels of carboxylic acids in urine. Due to the small population of children taking the supplement, further study are needed.
{"title":"Urinary carboxylic acids (UCAs) in subjects with autism spectrum disorder and their association with bacterial overgrowth","authors":"Paulina Gątarek, Jagoda Jóźwik-Pruska, Geir Bjorklund, S. Chirumbolo, J. Kałużna-Czaplińska","doi":"10.1515/revac-2020-0109","DOIUrl":"https://doi.org/10.1515/revac-2020-0109","url":null,"abstract":"Abstract In this study, the levels of concentration of carboxylic acids (benzoic acid, p-hydroxybenzoic acid, p-hydroxyphenylacetic acid, and hippuric acid) in the urine of autistic children were investigated and compared. The increased excretion of carboxylic acids is related to excessive bacterial activity in the gut, called bacterial overgrowth, which has been related to autism spectrum disorder (ASD) as an impairment in the gut-brain axis. The investigation was based on the analysis of urine samples obtained from 120 ASD children. To identify and quantify urinary carboxylic acids (UCAs), we applied gas chromatography coupled with mass spectrometry (GC-MS). Additionally, we checked the influence of probiotic supplementation, gender, body mass index (BMI) value and age of children on the level of different selected compounds. Most of the obtained results were found within reference ranges. In some cases, the levels of benzoic acid, p-hydroxybenzoic acid, and p-hydroxyphenylacetic acid were particularly elevated. Statistically significant differences were observed in supplementation with probiotics and the level of p-hydroxyphenylacetic acid (p=0.036). The obtained results may indicate disturbances in the intestinal flora in some autistic patients and suggest that supplements may have an influence on the levels of carboxylic acids in urine. Due to the small population of children taking the supplement, further study are needed.","PeriodicalId":21090,"journal":{"name":"Reviews in Analytical Chemistry","volume":"41 1","pages":"78 - 87"},"PeriodicalIF":4.3,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76794990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract With the progress of analysis technology and nanotechnology, colorimetric detection has become one of the research hotspots in the field of analytical chemistry. Compared with traditional detection methods, the colorimetric method has many advantages, such as high sensitivity, good selectivity, convenience and fast, as well as low cost. In recent years, metal nanoparticles have been introduced into colorimetry, making the research and application of colorimetry develop rapidly. In this work, we summarize the usual colorimetric detection methods based on metal nanoparticles-based nanozymes and their applications in the last five years. We hope that this work will help readers understand the mechanism and practical application value of nanozyme-based colorimetric biosensors. Meanwhile, this work may give some hints and references for future colorimetric detection research to promote the application and development of nanozyme-based colorimetry in biomedical and environmental analysis.
{"title":"Metal nanoparticles-based nanoplatforms for colorimetric sensing: A review","authors":"Ningtao Xu, S. Jin, Li Wang","doi":"10.1515/revac-2021-0122","DOIUrl":"https://doi.org/10.1515/revac-2021-0122","url":null,"abstract":"Abstract With the progress of analysis technology and nanotechnology, colorimetric detection has become one of the research hotspots in the field of analytical chemistry. Compared with traditional detection methods, the colorimetric method has many advantages, such as high sensitivity, good selectivity, convenience and fast, as well as low cost. In recent years, metal nanoparticles have been introduced into colorimetry, making the research and application of colorimetry develop rapidly. In this work, we summarize the usual colorimetric detection methods based on metal nanoparticles-based nanozymes and their applications in the last five years. We hope that this work will help readers understand the mechanism and practical application value of nanozyme-based colorimetric biosensors. Meanwhile, this work may give some hints and references for future colorimetric detection research to promote the application and development of nanozyme-based colorimetry in biomedical and environmental analysis.","PeriodicalId":21090,"journal":{"name":"Reviews in Analytical Chemistry","volume":"41 1","pages":"1 - 11"},"PeriodicalIF":4.3,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89133043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
B. Bobrowska-Korczak, K. Domanska, D. Skrajnowska, R. Wrzesień, J. Giebułtowicz, W. Bielecki, Rafał Wyrębiak, U. Piotrowska, M. Sobczak, J. Kałużna-Czaplińska
Abstract The aim of the research was to compare the impact of nano- and micro-sized-zinc on the kinetics of changes in the level of 3-methyladenine, 7-methylguanine, 7-methylguanosine, O-methylguanosine, 1-methyladenosine, N6-methyl-2’-deoxyguanosine in urine of rats with breast cancer. Female Sprague-Dawley rats divided into 3 groups were used in the study. Animals were fed only a control diet or diets supplemented with the nano and micro-sized zinc particles. To induce the mammary cancer (adenocarcinoma), rats were treated with 7,12-dimethylbenz[a]anthracene (DMBA). Modified nucleosides were determined by a validated high performance liquid chromatography coupled to mass spectrometry method. In the first stage of investigations a synergistic activity of nanosized Zn with DMBA on the growth of the neoplastic process was found. During that time a statistically significant increase in the levels of all six examined markers in the rats’ urine was observed. However, as the experiment continued, the supplementation with nanosized zinc caused inhibition of tumour growth, being followed by regression and remission of tumours, as well as, a statistically significant systematic reduction of the levels of methyl derivatives in the urine. Biopsy images indicated grade 1 tumours with multiple inflammatory infiltrates in the group treated with zinc nanoparticles, whereas, in the other groups, moderately-differentiated grade 2 adenocarcinoma was identified. It was found that the biological activity of zinc depends on the size of applied particles, as the treatment with zinc microparticles has not had much effect on cancer progression.
{"title":"Nanosized zinc, epigenetic changes and its relationship with DMBA induced breast cancer in rats","authors":"B. Bobrowska-Korczak, K. Domanska, D. Skrajnowska, R. Wrzesień, J. Giebułtowicz, W. Bielecki, Rafał Wyrębiak, U. Piotrowska, M. Sobczak, J. Kałużna-Czaplińska","doi":"10.1515/revac-2020-0104","DOIUrl":"https://doi.org/10.1515/revac-2020-0104","url":null,"abstract":"Abstract The aim of the research was to compare the impact of nano- and micro-sized-zinc on the kinetics of changes in the level of 3-methyladenine, 7-methylguanine, 7-methylguanosine, O-methylguanosine, 1-methyladenosine, N6-methyl-2’-deoxyguanosine in urine of rats with breast cancer. Female Sprague-Dawley rats divided into 3 groups were used in the study. Animals were fed only a control diet or diets supplemented with the nano and micro-sized zinc particles. To induce the mammary cancer (adenocarcinoma), rats were treated with 7,12-dimethylbenz[a]anthracene (DMBA). Modified nucleosides were determined by a validated high performance liquid chromatography coupled to mass spectrometry method. In the first stage of investigations a synergistic activity of nanosized Zn with DMBA on the growth of the neoplastic process was found. During that time a statistically significant increase in the levels of all six examined markers in the rats’ urine was observed. However, as the experiment continued, the supplementation with nanosized zinc caused inhibition of tumour growth, being followed by regression and remission of tumours, as well as, a statistically significant systematic reduction of the levels of methyl derivatives in the urine. Biopsy images indicated grade 1 tumours with multiple inflammatory infiltrates in the group treated with zinc nanoparticles, whereas, in the other groups, moderately-differentiated grade 2 adenocarcinoma was identified. It was found that the biological activity of zinc depends on the size of applied particles, as the treatment with zinc microparticles has not had much effect on cancer progression.","PeriodicalId":21090,"journal":{"name":"Reviews in Analytical Chemistry","volume":"115 1","pages":"200 - 208"},"PeriodicalIF":4.3,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86291529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Xiangqun Zheng, Wenyu Cheng, Chendong Ji, Jin Zhang, Meizhen Yin
Abstract Metal ions are widely present in biological systems and participate in many critical biochemical processes such as material transportation, energy conversion, information transmission and metabolic regulation, making them indispensable substance in our body. They can cause health problems when deficiency or excess occurs. To understand various metabolic processes and facilitate diseases diagnosis, it is very important to measure the content and monitor the distribution of metal ions in individual cells, tissues and whole organisms. Among the various methods for metal ion detection, fluorescent sensors with organic dyes have attracted tremendous attention due to many advantages such as high fluorescence quantum yield, facile modification approaches and biocompatibility in addition to operation ease, high sensitivity, fast detection speed, and real-time detection. This review summarizes the recent progress on the detection and imaging of the metal ions in biological systems including Na+, K+, Ca2+, Mg2+, Fe2+/Fe3+, Zn2+, and Cu2+ provides an opinion on remaining challenges to be addressed in this field.
{"title":"Detection of metal ions in biological systems: A review","authors":"Xiangqun Zheng, Wenyu Cheng, Chendong Ji, Jin Zhang, Meizhen Yin","doi":"10.1515/revac-2020-0118","DOIUrl":"https://doi.org/10.1515/revac-2020-0118","url":null,"abstract":"Abstract Metal ions are widely present in biological systems and participate in many critical biochemical processes such as material transportation, energy conversion, information transmission and metabolic regulation, making them indispensable substance in our body. They can cause health problems when deficiency or excess occurs. To understand various metabolic processes and facilitate diseases diagnosis, it is very important to measure the content and monitor the distribution of metal ions in individual cells, tissues and whole organisms. Among the various methods for metal ion detection, fluorescent sensors with organic dyes have attracted tremendous attention due to many advantages such as high fluorescence quantum yield, facile modification approaches and biocompatibility in addition to operation ease, high sensitivity, fast detection speed, and real-time detection. This review summarizes the recent progress on the detection and imaging of the metal ions in biological systems including Na+, K+, Ca2+, Mg2+, Fe2+/Fe3+, Zn2+, and Cu2+ provides an opinion on remaining challenges to be addressed in this field.","PeriodicalId":21090,"journal":{"name":"Reviews in Analytical Chemistry","volume":"17 1","pages":"231 - 246"},"PeriodicalIF":4.3,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82157187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-01Epub Date: 2020-12-31DOI: 10.1515/revac-2020-0117
Juan M Rojas Cabrera, J Blair Price, Aaron E Rusheen, Abhinav Goyal, Danielle Jondal, Abhijeet S Barath, Hojin Shin, Su-Youne Chang, Kevin E Bennet, Charles D Blaha, Kendall H Lee, Yoonbae Oh
Neurochemical recording techniques have expanded our understanding of the pathophysiology of neurological disorders, as well as the mechanisms of action of treatment modalities like deep brain stimulation (DBS). DBS is used to treat diseases such as Parkinson's disease, Tourette syndrome, and obsessive-compulsive disorder, among others. Although DBS is effective at alleviating symptoms related to these diseases and improving the quality of life of these patients, the mechanism of action of DBS is currently not fully understood. A leading hypothesis is that DBS modulates the electrical field potential by modifying neuronal firing frequencies to non-pathological rates thus providing therapeutic relief. To address this gap in knowledge, recent advances in electrochemical sensing techniques have given insight into the importance of neurotransmitters, such as dopamine, serotonin, glutamate, and adenosine, in disease pathophysiology. These studies have also highlighted their potential use in tandem with electrophysiology to serve as biomarkers in disease diagnosis and progression monitoring, as well as characterize response to treatment. Here, we provide an overview of disease-relevant neurotransmitters and their roles and implications as biomarkers, as well as innovations to the biosensors used to record these biomarkers. Furthermore, we discuss currently available neurochemical and electrophysiological recording devices, and discuss their viability to be implemented into the development of a closed-loop DBS system.
{"title":"Advances in neurochemical measurements: A review of biomarkers and devices for the development of closed-loop deep brain stimulation systems.","authors":"Juan M Rojas Cabrera, J Blair Price, Aaron E Rusheen, Abhinav Goyal, Danielle Jondal, Abhijeet S Barath, Hojin Shin, Su-Youne Chang, Kevin E Bennet, Charles D Blaha, Kendall H Lee, Yoonbae Oh","doi":"10.1515/revac-2020-0117","DOIUrl":"10.1515/revac-2020-0117","url":null,"abstract":"<p><p>Neurochemical recording techniques have expanded our understanding of the pathophysiology of neurological disorders, as well as the mechanisms of action of treatment modalities like deep brain stimulation (DBS). DBS is used to treat diseases such as Parkinson's disease, Tourette syndrome, and obsessive-compulsive disorder, among others. Although DBS is effective at alleviating symptoms related to these diseases and improving the quality of life of these patients, the mechanism of action of DBS is currently not fully understood. A leading hypothesis is that DBS modulates the electrical field potential by modifying neuronal firing frequencies to non-pathological rates thus providing therapeutic relief. To address this gap in knowledge, recent advances in electrochemical sensing techniques have given insight into the importance of neurotransmitters, such as dopamine, serotonin, glutamate, and adenosine, in disease pathophysiology. These studies have also highlighted their potential use in tandem with electrophysiology to serve as biomarkers in disease diagnosis and progression monitoring, as well as characterize response to treatment. Here, we provide an overview of disease-relevant neurotransmitters and their roles and implications as biomarkers, as well as innovations to the biosensors used to record these biomarkers. Furthermore, we discuss currently available neurochemical and electrophysiological recording devices, and discuss their viability to be implemented into the development of a closed-loop DBS system.</p>","PeriodicalId":21090,"journal":{"name":"Reviews in Analytical Chemistry","volume":"39 1","pages":"188-199"},"PeriodicalIF":4.3,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8057673/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38896246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jiafeng Wan, Xiaoyuan Zhang, Kai Zhang, Zhiqiang Su
Abstract In recent years, nanomaterials have attracted lots of attention from researchers due to their unique properties. Nanometer fluorescent materials, such as organic dyes, semiconductor quantum dots (QDs), metal nano-clusters (MNCs), carbon dots (CDs), etc., are widely used in biological imaging due to their high sensitivity, short response time, and excellent accuracy. Nanometer fluorescent probes can not only perform in vitro imaging of organisms but also achieve in vivo imaging. This provides medical staff with great convenience in cancer treatment. Combined with contemporary medical methods, faster and more effective treatment of cancer is achievable. This article explains the response mechanism of three-nanometer fluorescent probes: the principle of induced electron transfer (PET), the principle of fluorescence resonance energy transfer (FRET), and the principle of intramolecular charge transfer (ICT), showing the semiconductor QDs, precious MNCs, and CDs. The excellent performance of the three kinds of nano fluorescent materials in biological imaging is highlighted, and the application of these three kinds of nano fluorescent probes in targeted biological imaging is also introduced. Nanometer fluorescent materials will show their significance in the field of biomedicine.
{"title":"Biological nanoscale fluorescent probes: From structure and performance to bioimaging","authors":"Jiafeng Wan, Xiaoyuan Zhang, Kai Zhang, Zhiqiang Su","doi":"10.1515/revac-2020-0119","DOIUrl":"https://doi.org/10.1515/revac-2020-0119","url":null,"abstract":"Abstract In recent years, nanomaterials have attracted lots of attention from researchers due to their unique properties. Nanometer fluorescent materials, such as organic dyes, semiconductor quantum dots (QDs), metal nano-clusters (MNCs), carbon dots (CDs), etc., are widely used in biological imaging due to their high sensitivity, short response time, and excellent accuracy. Nanometer fluorescent probes can not only perform in vitro imaging of organisms but also achieve in vivo imaging. This provides medical staff with great convenience in cancer treatment. Combined with contemporary medical methods, faster and more effective treatment of cancer is achievable. This article explains the response mechanism of three-nanometer fluorescent probes: the principle of induced electron transfer (PET), the principle of fluorescence resonance energy transfer (FRET), and the principle of intramolecular charge transfer (ICT), showing the semiconductor QDs, precious MNCs, and CDs. The excellent performance of the three kinds of nano fluorescent materials in biological imaging is highlighted, and the application of these three kinds of nano fluorescent probes in targeted biological imaging is also introduced. Nanometer fluorescent materials will show their significance in the field of biomedicine.","PeriodicalId":21090,"journal":{"name":"Reviews in Analytical Chemistry","volume":"79 1","pages":"209 - 221"},"PeriodicalIF":4.3,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86890014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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