Abstract In order to enhance the service life of special complex shaped workpieces under severe working conditions, a new coating preparation process, namely, easy-coating and sintering technology, has been proposed. In this study, the feasibility of preparing Co-based coatings on 38CrMoAl substrates using the easy-coating and sintering process was investigated, and the microstructure and properties of the Co-based coatings were studied. By optimizing the parameters of the sintering process, the prepared coating is dense, and the coating forms a good metallurgical bond with the substrate with few pores, but no other defects. As the process has good applicability to the surface of complex-shaped parts, the inner wall of small serpentine tubes and large thicknesses, and is low-cost and easy to operate, the easy-coating-sintering process has great application prospects.
{"title":"Fabrications and microstructure analysis of cobalt-based coatings by an easy-coating and sintering process","authors":"Lingyu Meng, M. Hu, Keming Jia","doi":"10.1515/secm-2022-0178","DOIUrl":"https://doi.org/10.1515/secm-2022-0178","url":null,"abstract":"Abstract In order to enhance the service life of special complex shaped workpieces under severe working conditions, a new coating preparation process, namely, easy-coating and sintering technology, has been proposed. In this study, the feasibility of preparing Co-based coatings on 38CrMoAl substrates using the easy-coating and sintering process was investigated, and the microstructure and properties of the Co-based coatings were studied. By optimizing the parameters of the sintering process, the prepared coating is dense, and the coating forms a good metallurgical bond with the substrate with few pores, but no other defects. As the process has good applicability to the surface of complex-shaped parts, the inner wall of small serpentine tubes and large thicknesses, and is low-cost and easy to operate, the easy-coating-sintering process has great application prospects.","PeriodicalId":21480,"journal":{"name":"Science and Engineering of Composite Materials","volume":"29 1","pages":"529 - 534"},"PeriodicalIF":1.9,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46107405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract This article focuses on an experimental study of the flexural response of a polymer composite laminate (CL) reinforced with a plain weave carbon fiber fabric. The aim of this research is to investigate the effect of the span length-to-specimen thickness ratio on the flexural properties of the selected CL. The laminate specimens were tested in the three-point flexure configuration for span length-to-specimen thickness ratios of 16, 20, 32, 40 and 60. The investigations were carried out to identify on-axis and 45° off-axis flexural responses of the CL. For this reason, two types of rectangular flat specimens were prepared, differing in reinforcement orientation relative to the support span. Using the special code, the lay-up of the specimens was designated as [(0/90)F]8 and [±45 F]8, respectively. It was found that the flexural modulus and flexural strength of the specimens depend on the span length-to-specimen thickness ratio. Despite testing the same CL, the response of the specimens to the change in the span length is different. In addition, it was concluded that the span length also has a significant influence on the final failure of the laminate specimens.
{"title":"Span length effect on flexural properties of composite laminate reinforced with a plain weave carbon fiber fabric in a polymer matrix","authors":"J. Marszałek","doi":"10.1515/secm-2022-0155","DOIUrl":"https://doi.org/10.1515/secm-2022-0155","url":null,"abstract":"Abstract This article focuses on an experimental study of the flexural response of a polymer composite laminate (CL) reinforced with a plain weave carbon fiber fabric. The aim of this research is to investigate the effect of the span length-to-specimen thickness ratio on the flexural properties of the selected CL. The laminate specimens were tested in the three-point flexure configuration for span length-to-specimen thickness ratios of 16, 20, 32, 40 and 60. The investigations were carried out to identify on-axis and 45° off-axis flexural responses of the CL. For this reason, two types of rectangular flat specimens were prepared, differing in reinforcement orientation relative to the support span. Using the special code, the lay-up of the specimens was designated as [(0/90)F]8 and [±45 F]8, respectively. It was found that the flexural modulus and flexural strength of the specimens depend on the span length-to-specimen thickness ratio. Despite testing the same CL, the response of the specimens to the change in the span length is different. In addition, it was concluded that the span length also has a significant influence on the final failure of the laminate specimens.","PeriodicalId":21480,"journal":{"name":"Science and Engineering of Composite Materials","volume":"29 1","pages":"322 - 334"},"PeriodicalIF":1.9,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46710203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yinghao Cui, Cong Xie, Jialin Liu, Shi-rui Guo, Lu-jun Cui
Abstract To improve the mechanical properties and corrosion resistance of the hydraulic column in the mine, a semiconductor fiber-coupled laser was used to laser-clad iron-based alloy powder on a 27SiMn steel substrate (SUB). The microstructure, mechanical properties, and corrosion resistance of the obtained cladding layer (CL) were studied based on experiments. Results show that equiaxed grains at the top of the CL are fine and tightly arranged. Compared with the SUB, the CL average microhardness is increased by 0.3 times, the average friction coefficient is decreased by 0.12, and the wear amount is significantly reduced. The bonding strength between the CL and the SUB is good, and the tensile strength is increased by 10.82%. Compared with the SUB, the mechanical properties and corrosion resistance of the CL are better. The research has an important guiding significance for the practice of hydraulic column repair and strengthening engineering.
{"title":"Mechanical and corrosion resistance analysis of laser cladding layer","authors":"Yinghao Cui, Cong Xie, Jialin Liu, Shi-rui Guo, Lu-jun Cui","doi":"10.1515/secm-2022-0161","DOIUrl":"https://doi.org/10.1515/secm-2022-0161","url":null,"abstract":"Abstract To improve the mechanical properties and corrosion resistance of the hydraulic column in the mine, a semiconductor fiber-coupled laser was used to laser-clad iron-based alloy powder on a 27SiMn steel substrate (SUB). The microstructure, mechanical properties, and corrosion resistance of the obtained cladding layer (CL) were studied based on experiments. Results show that equiaxed grains at the top of the CL are fine and tightly arranged. Compared with the SUB, the CL average microhardness is increased by 0.3 times, the average friction coefficient is decreased by 0.12, and the wear amount is significantly reduced. The bonding strength between the CL and the SUB is good, and the tensile strength is increased by 10.82%. Compared with the SUB, the mechanical properties and corrosion resistance of the CL are better. The research has an important guiding significance for the practice of hydraulic column repair and strengthening engineering.","PeriodicalId":21480,"journal":{"name":"Science and Engineering of Composite Materials","volume":"29 1","pages":"358 - 363"},"PeriodicalIF":1.9,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47101181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract A theoretical framework that underlies an accelerated testing method for unidirectional (UD) composites is proposed in this article, based on the continuum damage mechanics and time–temperature superposition. By the way of demonstration, it has been applied to problems involving constant strain rates and fatigue loading conditions. The damage evolution law for the matrix of composites was constructed using the Weibull distribution of defects, which will develop into cracks as a result of deformation. In conjunction with the Wiechert model, the theoretical framework formulated here is capable of capturing the mechanical behaviour of UD composites. Demonstration and initial verification of this acceleration method were carried out through experiments. This work can provide theoretical and technical support for the durability verification and the evaluation of the high cycle fatigue performance of composite structures.
{"title":"A theoretical framework underlying an accelerated testing method and its application to composites under constant strain rates and fatigue loading","authors":"Fei Xu, I. Arthur Jones, Shuguang Li","doi":"10.1515/secm-2022-0012","DOIUrl":"https://doi.org/10.1515/secm-2022-0012","url":null,"abstract":"Abstract A theoretical framework that underlies an accelerated testing method for unidirectional (UD) composites is proposed in this article, based on the continuum damage mechanics and time–temperature superposition. By the way of demonstration, it has been applied to problems involving constant strain rates and fatigue loading conditions. The damage evolution law for the matrix of composites was constructed using the Weibull distribution of defects, which will develop into cracks as a result of deformation. In conjunction with the Wiechert model, the theoretical framework formulated here is capable of capturing the mechanical behaviour of UD composites. Demonstration and initial verification of this acceleration method were carried out through experiments. This work can provide theoretical and technical support for the durability verification and the evaluation of the high cycle fatigue performance of composite structures.","PeriodicalId":21480,"journal":{"name":"Science and Engineering of Composite Materials","volume":"29 1","pages":"488 - 499"},"PeriodicalIF":1.9,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42289709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yuan Liu, Wen-jun Li, Yan-Ling Cui, Yukun Yang, Jipeng Yang
Abstract Graphite flakes/Al composites are promising thermal management materials due to high thermal conductivity (TC) in basal plane orientation, matched coefficient of thermal expansion, and good machinability. In this article, the acoustic mismatch model and the effective medium approach are applied to predict the influence of different interfacial coatings on the interfacial thermal conductance (ITC) and the TC of graphite flakes/Al composites, respectively. With the increase in the thickness of interfacial coatings, the ITC and the TC of graphite flakes/Al composites decrease. For the composites with Ni, Cr/Cr7C3/Cr3C2, Si/SiC, Ti/TiC, WC, and Mo/Mo2C coatings, the ITC is sensitive to coating thickness. In order to obtain ideal TC of graphite flakes/Al composites, the thickness of the coatings should be controlled below 1 μm. It is reasonable that the TC of the graphite flakes/Al composites increases as the volume fraction of graphite flakes increases. The TC of the graphite flakes/Al composites increases with the ITC and changes slowly when the ITC increases to a certain extent. Si/SiC and WC coatings are proposed to be the most promising candidates to improve the thermal performance of graphite flakes/Al composites.
{"title":"Theoretical analysis of interfacial design and thermal conductivity in graphite flakes/Al composites with various interfacial coatings","authors":"Yuan Liu, Wen-jun Li, Yan-Ling Cui, Yukun Yang, Jipeng Yang","doi":"10.1515/secm-2022-0152","DOIUrl":"https://doi.org/10.1515/secm-2022-0152","url":null,"abstract":"Abstract Graphite flakes/Al composites are promising thermal management materials due to high thermal conductivity (TC) in basal plane orientation, matched coefficient of thermal expansion, and good machinability. In this article, the acoustic mismatch model and the effective medium approach are applied to predict the influence of different interfacial coatings on the interfacial thermal conductance (ITC) and the TC of graphite flakes/Al composites, respectively. With the increase in the thickness of interfacial coatings, the ITC and the TC of graphite flakes/Al composites decrease. For the composites with Ni, Cr/Cr7C3/Cr3C2, Si/SiC, Ti/TiC, WC, and Mo/Mo2C coatings, the ITC is sensitive to coating thickness. In order to obtain ideal TC of graphite flakes/Al composites, the thickness of the coatings should be controlled below 1 μm. It is reasonable that the TC of the graphite flakes/Al composites increases as the volume fraction of graphite flakes increases. The TC of the graphite flakes/Al composites increases with the ITC and changes slowly when the ITC increases to a certain extent. Si/SiC and WC coatings are proposed to be the most promising candidates to improve the thermal performance of graphite flakes/Al composites.","PeriodicalId":21480,"journal":{"name":"Science and Engineering of Composite Materials","volume":"29 1","pages":"500 - 507"},"PeriodicalIF":1.9,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45197550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Lou, Xin Li, G. Guo, Ling Ran, Yongqiang Liu, P. Zhang, C. Su
Abstract Through hot compression experiments at temperatures ranging from 603 to 723 K and strain rates ranging from 0.01 to 10 s−1, the hot deformation behavior of a 0.5 wt% graphene nanoplatelet-reinforced aluminum (0.5 wt% GNP/Al) composite prepared by the powder metallurgy method was studied. The constitutive equations obtained by mathematical models and a neural network were evaluated. The deformation property of the composite can be better described by the Johnson–Cook (JC) constitutive model optimized by establishing a relationship between the coefficient and variables obtained in the hot compression test, with a correlation coefficient (R) reaching 99.97% with the average relative error of 0.37% (98.1 and 4.17%, respectively, before optimization). Compared with the JC model, the neural network has perfect calculation accuracy and whole-process effectiveness, providing expanded and more accurate constitutive equations for subsequent simulations and for building the dynamic recrystallization model of the composite. The dynamic recrystallization model, hot processing map, and EBSD results are in agreement with each other and indicate that the optimal strain rate and temperature range of the composite are 0.01–0.1 s−1 and 693–723 K, respectively.
{"title":"Hot deformation behavior and microstructure of a 0.5 wt% graphene nanoplatelet reinforced aluminum composite","authors":"S. Lou, Xin Li, G. Guo, Ling Ran, Yongqiang Liu, P. Zhang, C. Su","doi":"10.1515/secm-2022-0009","DOIUrl":"https://doi.org/10.1515/secm-2022-0009","url":null,"abstract":"Abstract Through hot compression experiments at temperatures ranging from 603 to 723 K and strain rates ranging from 0.01 to 10 s−1, the hot deformation behavior of a 0.5 wt% graphene nanoplatelet-reinforced aluminum (0.5 wt% GNP/Al) composite prepared by the powder metallurgy method was studied. The constitutive equations obtained by mathematical models and a neural network were evaluated. The deformation property of the composite can be better described by the Johnson–Cook (JC) constitutive model optimized by establishing a relationship between the coefficient and variables obtained in the hot compression test, with a correlation coefficient (R) reaching 99.97% with the average relative error of 0.37% (98.1 and 4.17%, respectively, before optimization). Compared with the JC model, the neural network has perfect calculation accuracy and whole-process effectiveness, providing expanded and more accurate constitutive equations for subsequent simulations and for building the dynamic recrystallization model of the composite. The dynamic recrystallization model, hot processing map, and EBSD results are in agreement with each other and indicate that the optimal strain rate and temperature range of the composite are 0.01–0.1 s−1 and 693–723 K, respectively.","PeriodicalId":21480,"journal":{"name":"Science and Engineering of Composite Materials","volume":"29 1","pages":"97 - 112"},"PeriodicalIF":1.9,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49616163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jianhui Tian, Hongrui Zhang, Jinjuan Sun, Jialiang Wu, G. Hu
Abstract This article used the strip element method to study the dynamics problems of the functionally graded plate with variable gradient parameters under the harmonic loads. The dynamic model of the functionally graded plate is established by using the strip element method, the rationality and accuracy of the theoretical results are verified by finite element method, and the displacement response under different gradient parameters is also calculated. The results show that under the different gradient parameters, the displacement varies harmonically with time, and with the increase of gradient parameters, the fluctuation period of displacement with time increases continuously, and the displacement peak also gradually increases. The displacement along the thickness direction also shows the harmonic form. Through comparison, it is found that the gradient parameters have a greater impact on the dynamic response for the functionally graded plate; with the increase in the gradient parameters, the displacement response also increases, but the displacement response trend slows down.
{"title":"Dynamic response of functionally graded plate under harmonic load with variable gradient parameters","authors":"Jianhui Tian, Hongrui Zhang, Jinjuan Sun, Jialiang Wu, G. Hu","doi":"10.1515/secm-2022-0019","DOIUrl":"https://doi.org/10.1515/secm-2022-0019","url":null,"abstract":"Abstract This article used the strip element method to study the dynamics problems of the functionally graded plate with variable gradient parameters under the harmonic loads. The dynamic model of the functionally graded plate is established by using the strip element method, the rationality and accuracy of the theoretical results are verified by finite element method, and the displacement response under different gradient parameters is also calculated. The results show that under the different gradient parameters, the displacement varies harmonically with time, and with the increase of gradient parameters, the fluctuation period of displacement with time increases continuously, and the displacement peak also gradually increases. The displacement along the thickness direction also shows the harmonic form. Through comparison, it is found that the gradient parameters have a greater impact on the dynamic response for the functionally graded plate; with the increase in the gradient parameters, the displacement response also increases, but the displacement response trend slows down.","PeriodicalId":21480,"journal":{"name":"Science and Engineering of Composite Materials","volume":"29 1","pages":"183 - 193"},"PeriodicalIF":1.9,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44557105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yong-he Li, Qingguo Wang, Lina Tuo, Fasong Zhang, Xiaoyu Zhang
Abstract Compared with large size foam, pressure microfoam has the characteristics of a good pipeline transportation stability, good jet orientation, strong impact force, and strong ability to capture fine dust, which is more suitable for dust removal. The traditional foam preparation device has the disadvantages of high-pressure loss, poor foaming effect, and foam uniformity. To overcome the above shortcomings, the annular air supply vertical foam preparation device was proposed in the article. The foaming cylinder of the device adopts a vertical design to avoid the influence of uneven distribution of foam caused by gravity; the spiral nozzle is used to evenly spray the foaming liquid on the foaming mesh to increase the contact area between foam and airflow. The stainless steel wire mesh and cotton wire mesh are adopted to improve the reliability and durability of concave foaming mesh. The performance of the new device was obtained by the self-built experimental system. Finally, the field test shows that the conditions of the heading face could fully meet the requirements of the device for pressure water and compressed air, and the produced pressure microfoam can effectively control dust.
{"title":"Experimental investigations of a novel pressure microfoam preparation device for dust removal","authors":"Yong-he Li, Qingguo Wang, Lina Tuo, Fasong Zhang, Xiaoyu Zhang","doi":"10.1515/secm-2022-0001","DOIUrl":"https://doi.org/10.1515/secm-2022-0001","url":null,"abstract":"Abstract Compared with large size foam, pressure microfoam has the characteristics of a good pipeline transportation stability, good jet orientation, strong impact force, and strong ability to capture fine dust, which is more suitable for dust removal. The traditional foam preparation device has the disadvantages of high-pressure loss, poor foaming effect, and foam uniformity. To overcome the above shortcomings, the annular air supply vertical foam preparation device was proposed in the article. The foaming cylinder of the device adopts a vertical design to avoid the influence of uneven distribution of foam caused by gravity; the spiral nozzle is used to evenly spray the foaming liquid on the foaming mesh to increase the contact area between foam and airflow. The stainless steel wire mesh and cotton wire mesh are adopted to improve the reliability and durability of concave foaming mesh. The performance of the new device was obtained by the self-built experimental system. Finally, the field test shows that the conditions of the heading face could fully meet the requirements of the device for pressure water and compressed air, and the produced pressure microfoam can effectively control dust.","PeriodicalId":21480,"journal":{"name":"Science and Engineering of Composite Materials","volume":"29 1","pages":"1 - 8"},"PeriodicalIF":1.9,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43316568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ran Yang, Jigang Zhang, Chenghao Zhou, Wenli Chen, Jiguang Chen, Yang Zhao
Abstract Honeycomb materials are widely used across engineering fields. The use of honeycomb sandwich structures as energy dissipation and impact protection materials for reinforced concrete components in the field of civil engineering is a novel concept. Therefore, it is of great significance to study the energy absorption characteristics, dynamic response theoretical model, and collapse energy dissipation theory for the use of honeycomb sandwich structures as protective materials. Based on the large-scale low-speed pendulum impact test and the corresponding finite element model, this study establishes an ideal model for the honeycomb sandwich panel under the impact of a square flat head and gives the corresponding theoretical derivation. At the same time, it puts forward a method to estimate the energy consumption and dynamic crushing distance based on the energy consumption theory angle of the covering layer.
{"title":"Theoretical analysis of aluminum honeycomb sandwich panel supported by reinforced concrete wall under low-speed impact load","authors":"Ran Yang, Jigang Zhang, Chenghao Zhou, Wenli Chen, Jiguang Chen, Yang Zhao","doi":"10.1515/secm-2022-0150","DOIUrl":"https://doi.org/10.1515/secm-2022-0150","url":null,"abstract":"Abstract Honeycomb materials are widely used across engineering fields. The use of honeycomb sandwich structures as energy dissipation and impact protection materials for reinforced concrete components in the field of civil engineering is a novel concept. Therefore, it is of great significance to study the energy absorption characteristics, dynamic response theoretical model, and collapse energy dissipation theory for the use of honeycomb sandwich structures as protective materials. Based on the large-scale low-speed pendulum impact test and the corresponding finite element model, this study establishes an ideal model for the honeycomb sandwich panel under the impact of a square flat head and gives the corresponding theoretical derivation. At the same time, it puts forward a method to estimate the energy consumption and dynamic crushing distance based on the energy consumption theory angle of the covering layer.","PeriodicalId":21480,"journal":{"name":"Science and Engineering of Composite Materials","volume":"29 1","pages":"265 - 273"},"PeriodicalIF":1.9,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47395140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract Objective To prove that calcium alginate beads can be used as scaffolds during in vitro culture. Methods Mouse preosteoblastic cells (MC3T3-E1) were encapsulated in calcium alginate hydrogel beads. The Cell Counting Kit-8 (CCK-8) assay was used to assess cell viability at 2, 5, 8, 11, 14, and 21 days. Calcein-AM and propidium iodide (PI) were employed for live/dead staining. Results MC3T3-E1 cells were alive on day 21 and had the highest viability on day 14. Conclusion MC3T3-E1 cells could be encapsulated in calcium alginate hydrogel beads and cultured. Calcium alginate hydrogel beads can be used as scaffolds for three-dimensional in vitro culture.
{"title":"The viability of cell that encapsulated in calcium alginate hydrogel beads","authors":"Fang-fang Li, W. Tang, Q. Xie","doi":"10.1515/secm-2022-0156","DOIUrl":"https://doi.org/10.1515/secm-2022-0156","url":null,"abstract":"Abstract Objective To prove that calcium alginate beads can be used as scaffolds during in vitro culture. Methods Mouse preosteoblastic cells (MC3T3-E1) were encapsulated in calcium alginate hydrogel beads. The Cell Counting Kit-8 (CCK-8) assay was used to assess cell viability at 2, 5, 8, 11, 14, and 21 days. Calcein-AM and propidium iodide (PI) were employed for live/dead staining. Results MC3T3-E1 cells were alive on day 21 and had the highest viability on day 14. Conclusion MC3T3-E1 cells could be encapsulated in calcium alginate hydrogel beads and cultured. Calcium alginate hydrogel beads can be used as scaffolds for three-dimensional in vitro culture.","PeriodicalId":21480,"journal":{"name":"Science and Engineering of Composite Materials","volume":"29 1","pages":"473 - 480"},"PeriodicalIF":1.9,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44893964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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