Pub Date : 2024-09-28DOI: 10.1016/j.scienta.2024.113677
Sanpeng Jin, Wei Hu, Jie Song, Dechun Liu, Liuqing Kuang, Jingheng Xie, Li Yang, Yong Liu
Lateral organ boundaries domain (LBD) is a plant-specific transcription factor. Here, we identified 37 LBD genes of sweet orange in the CPBD database, which were unevenly distributed on 7 chromosomes, and most of those were located in the nucleus. All CsLBD genes were divided into class I (31) containing five subclasses, and class II (6) containing two subclasses and associated with plant growth and development, plant hormones, defense and stress response. WGD/segmental duplication events primarily were confrimed to promote the development of the CsLBD gene family. CsLBDs were more strongly correlated with dicotyledonous plants than with monocotyledonous plants. Gene expression analysis showed that CsLBD16 and CsLBD17 genes in segment stem cultured in callus induction medium were higher than those in the other two media without auxin throughout the cultivation process. The N-terminal region of the CsLBD17 protein is responsible for the transcriptional activation of CsLBD17. Arabidopsis thaliana with overexpression of CsLBD17 showed stronger callus formation ability on CIM medium and stronger root growth ability on MS medium, indicating that it plays an important role in the development of calluses and roots.
{"title":"Genomic identification and expression profiles during callus formation of LBD transcription factor genes in sweet orange (Citrus sinensis) and functional characterization of CsLBD17","authors":"Sanpeng Jin, Wei Hu, Jie Song, Dechun Liu, Liuqing Kuang, Jingheng Xie, Li Yang, Yong Liu","doi":"10.1016/j.scienta.2024.113677","DOIUrl":"10.1016/j.scienta.2024.113677","url":null,"abstract":"<div><div>Lateral organ boundaries domain (LBD) is a plant-specific transcription factor. Here, we identified 37 <em>LBD</em> genes of sweet orange in the CPBD database, which were unevenly distributed on 7 chromosomes, and most of those were located in the nucleus. All <em>CsLBD</em> genes were divided into class I (31) containing five subclasses, and class II (6) containing two subclasses and associated with plant growth and development, plant hormones, defense and stress response. WGD/segmental duplication events primarily were confrimed to promote the development of the <em>CsLBD</em> gene family. <em>CsLBDs</em> were more strongly correlated with dicotyledonous plants than with monocotyledonous plants. Gene expression analysis showed that <em>CsLBD16</em> and <em>CsLBD17</em> genes in segment stem cultured in callus induction medium were higher than those in the other two media without auxin throughout the cultivation process. The N-terminal region of the CsLBD17 protein is responsible for the transcriptional activation of CsLBD17. <em>Arabidopsis thaliana</em> with overexpression of <em>CsLBD17</em> showed stronger callus formation ability on CIM medium and stronger root growth ability on MS medium, indicating that it plays an important role in the development of calluses and roots.</div></div>","PeriodicalId":21679,"journal":{"name":"Scientia Horticulturae","volume":"338 ","pages":"Article 113677"},"PeriodicalIF":3.9,"publicationDate":"2024-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142329660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In this study, we investigated the effect of heat exposure during olive inflorescence on reproductive development. We hypothesized that male development processes during this period are particularly sensitive to high temperatures. We characterized the development of flowers and inflorescences under natural conditions in the cultivars 'Arbequina' and 'Koroneiki', and defined eight phenological stages, beginning with the emergence of reproductive buds until anthesis (international standard BBCH scale of the phenological inflorescence development phases 53–60). In addition, we examined the response of olive inflorescence and flowering, to two heat scenarios: prolonged exposure to moderately high temperatures and a two-hour concentrated heat shock treatment. Analysis of our data revealed that both treatments had a detrimental effect on the development and functioning of the tapetum, the innermost layer of the anther, which is essential for the development and functioning of pollen. Additionally, we found that the 'Arbequina' cultivar displayed relative tolerance to heat compared to 'Koroneiki' in terms of pollen germinability after heat stress. In contrast to the response of the above cultivars, the 'Souri' exhibited an exceptionally high sensitivity to heat. An assessment of pollen viability after in vitro heat shock treatment of 54 cultivars revealed that pollen from seven varieties, 'Niedda de Oliana', 'Lechín de Sevilla', 'Tonda Oliana', 'Tamir', 'Sigoise', 'Taggiasca', and 'Carrasquenha', showed exceptional tolerance to heat. These cultivars show a greater degree of suitability for cultivation in warm climates, (or in areas susceptible to extreme hot-spells). This study demonstrated that heat has a negative impact on the male reproductive system in olives, during early flower development and in mature pollen grains, which are relatively resistant to heat. Heat stress impaired pollen tube elongation capacity, and germination rate. Additionally, heat stress affected the ultrastructure of the external wall of the pollen grain, the exine, potentially impairing its ability to adhere to the stigma and fertilize the ovule. The sensitivity of olive reproductive capacity to heat is a genotype-dependent trait, allowing for the identification of heat-resistant cultivars for use as pollen donors and for development of new heat-resistant cultivars.
{"title":"Heat stress during reproductive development inhibits fertilization in olives","authors":"Karen Wortmann , Iris Biton , Hanita Zemach , Yair Many , Giora Ben-Ari","doi":"10.1016/j.scienta.2024.113680","DOIUrl":"10.1016/j.scienta.2024.113680","url":null,"abstract":"<div><div>In this study, we investigated the effect of heat exposure during olive inflorescence on reproductive development. We hypothesized that male development processes during this period are particularly sensitive to high temperatures. We characterized the development of flowers and inflorescences under natural conditions in the cultivars 'Arbequina' and 'Koroneiki', and defined eight phenological stages, beginning with the emergence of reproductive buds until anthesis (international standard BBCH scale of the phenological inflorescence development phases 53–60). In addition, we examined the response of olive inflorescence and flowering, to two heat scenarios: prolonged exposure to moderately high temperatures and a two-hour concentrated heat shock treatment. Analysis of our data revealed that both treatments had a detrimental effect on the development and functioning of the tapetum, the innermost layer of the anther, which is essential for the development and functioning of pollen. Additionally, we found that the 'Arbequina' cultivar displayed relative tolerance to heat compared to 'Koroneiki' in terms of pollen germinability after heat stress. In contrast to the response of the above cultivars, the 'Souri' exhibited an exceptionally high sensitivity to heat. An assessment of pollen viability after in vitro heat shock treatment of 54 cultivars revealed that pollen from seven varieties, 'Niedda de Oliana', 'Lechín de Sevilla', 'Tonda Oliana', 'Tamir', 'Sigoise', 'Taggiasca', and 'Carrasquenha', showed exceptional tolerance to heat. These cultivars show a greater degree of suitability for cultivation in warm climates, (or in areas susceptible to extreme hot-spells). This study demonstrated that heat has a negative impact on the male reproductive system in olives, during early flower development and in mature pollen grains, which are relatively resistant to heat. Heat stress impaired pollen tube elongation capacity, and germination rate. Additionally, heat stress affected the ultrastructure of the external wall of the pollen grain, the exine, potentially impairing its ability to adhere to the stigma and fertilize the ovule. The sensitivity of olive reproductive capacity to heat is a genotype-dependent trait, allowing for the identification of heat-resistant cultivars for use as pollen donors and for development of new heat-resistant cultivars.</div></div>","PeriodicalId":21679,"journal":{"name":"Scientia Horticulturae","volume":"338 ","pages":"Article 113680"},"PeriodicalIF":3.9,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142329664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-27DOI: 10.1016/j.scienta.2024.113658
Francesco Abbatantuono , Giuseppe Lopriore , Anas Tallou , Luca Brillante , Salem Alhajj Ali , Salvatore Camposeo , Gaetano Alessandro Vivaldi
According to modern precision agriculture principles, remote and proximal sensing can be extraordinarily useful tools for sustainable water resource management in viticulture. More than one hundred papers were read and cataloged to outline the most effective methodology (comprised of platforms, cameras, indices, single bands, and statistical methods) for monitoring water status in different wine grape varieties located in different areas. Satellites and airplanes can monitor areas at the regional or larger scale; however, while satellite images can be free, airplane imagery can be more expensive. The use of satellite platforms is particularly promising, especially due to recent technical progress aimed at improving spatial and temporal resolution. In addition, unmanned aerial vehicles (aka drones) equipped with thermal, multispectral, and hyperspectral cameras have provided excellent results. Proximal thermal and spectral cameras (e.g., handheld or installed in tractors) can be an inexpensive alternative but often present similar problems to traditional methods (e.g., time-consuming). The best results were obtained from thermal indices (e.g., Crop Water Stress Index) and the use of machine learning (ML) algorithms on individual bands and indices obtained with hyperspectral or multispectral cameras carried on drone or satellite platforms.
{"title":"Recent progress on grapevine water status assessment through remote and proximal sensing: A review","authors":"Francesco Abbatantuono , Giuseppe Lopriore , Anas Tallou , Luca Brillante , Salem Alhajj Ali , Salvatore Camposeo , Gaetano Alessandro Vivaldi","doi":"10.1016/j.scienta.2024.113658","DOIUrl":"10.1016/j.scienta.2024.113658","url":null,"abstract":"<div><div>According to modern precision agriculture principles, remote and proximal sensing can be extraordinarily useful tools for sustainable water resource management in viticulture. More than one hundred papers were read and cataloged to outline the most effective methodology (comprised of platforms, cameras, indices, single bands, and statistical methods) for monitoring water status in different wine grape varieties located in different areas. Satellites and airplanes can monitor areas at the regional or larger scale; however, while satellite images can be free, airplane imagery can be more expensive. The use of satellite platforms is particularly promising, especially due to recent technical progress aimed at improving spatial and temporal resolution. In addition, unmanned aerial vehicles (aka drones) equipped with thermal, multispectral, and hyperspectral cameras have provided excellent results. Proximal thermal and spectral cameras (e.g., handheld or installed in tractors) can be an inexpensive alternative but often present similar problems to traditional methods (e.g., time-consuming). The best results were obtained from thermal indices (e.g., Crop Water Stress Index) and the use of machine learning (ML) algorithms on individual bands and indices obtained with hyperspectral or multispectral cameras carried on drone or satellite platforms.</div></div>","PeriodicalId":21679,"journal":{"name":"Scientia Horticulturae","volume":"338 ","pages":"Article 113658"},"PeriodicalIF":3.9,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142329691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-27DOI: 10.1016/j.scienta.2024.113668
Martina Puccinelli , Matteo Mei , Beatrice Pezzarossa , Irene Rosellini , Fernando Malorgio , Alberto Pardossi
The market of microgreens is rapidly growing due to their tenderness and concentrations of many beneficial substances. The range of plant species grown for microgreens production is large and still expanding. Atriplex hortensis var. rubra L. (red orache; RO) is characterised by high vitamin C content and red-purple leaves, and could replace other red-leaved plants in microgreen boxes. The nutraceutical profile of microgreens could be further enhanced by biofortification with essential micronutrients for humans, such as selenium (Se) and zinc (Zn). The aim of our study was to explore the potential of RO for the production of microgreens biofortified with selenium (Se) or zinc (Zn) in comparison with Swiss chard (Beta vulgaris ssp. vulgaris var. cicla; SC), which is a more commonly consumed microgreen. Microgreens were grown in coconut coir irrigated at sowing with water containing different concentrations of sodium selenate (Na2SeO4; 0, 1, 3, and 5 mg Se L-1) or zinc sulphate (ZnSO4; 0, 2, 6, and 10 mg Zn L-1). The impact of adding a solution containing different concentrations of Se (0, 1, 3, and 5 mg L-1 Se) or Zn (0, 2, 6, and 10 mg L-1 Zn) to the substrate, at the time of sowing, on the growth and nutraceutical characteristics of the microgreens of both species was investigated together with their potential contribution to the daily requirements of Se and Zn for humans. The fresh weight and concentration of pigments, antioxidant compounds, especially ascorbic acid, and nitrate, and the total antioxidant capacity of microgreens were significantly higher in RO than in SC. Treatment with Se or Zn did not significantly affect the yield and quality of SC and RO microgreens, while increasing the microgreen concentration of Se (from 0.57 to 6.96 mg kg-1 FW) or Zn (from 4.46 to 12.55 mg kg-1 FW), respectively. Biofortification was more effective with Se than with Zn. A serving dose of 10 g of microgreens biofortified with 5 mg L-1 of Se could meet the daily requirement of this element in humans. In order to have a significant input (15%) to the daily requirements of Zn from SC and RO microgreens obtained with 10 mg L-1 Zn, the serving dose should be at least 100 gr.
{"title":"Exploiting the potential of red orache (Atriplex hortensis var. rubra L.) for the production of microgreens biofortified with selenium or zinc in comparison with Swiss chard (Beta vulgaris ssp. vulgaris var. cicla)","authors":"Martina Puccinelli , Matteo Mei , Beatrice Pezzarossa , Irene Rosellini , Fernando Malorgio , Alberto Pardossi","doi":"10.1016/j.scienta.2024.113668","DOIUrl":"10.1016/j.scienta.2024.113668","url":null,"abstract":"<div><div>The market of microgreens is rapidly growing due to their tenderness and concentrations of many beneficial substances. The range of plant species grown for microgreens production is large and still expanding. <em>Atriplex hortensis</em> var. <em>rubra</em> L. (red orache; RO) is characterised by high vitamin C content and red-purple leaves, and could replace other red-leaved plants in microgreen boxes. The nutraceutical profile of microgreens could be further enhanced by biofortification with essential micronutrients for humans, such as selenium (Se) and zinc (Zn). The aim of our study was to explore the potential of RO for the production of microgreens biofortified with selenium (Se) or zinc (Zn) in comparison with Swiss chard (<em>Beta vulgaris</em> ssp. <em>vulgaris</em> var. <em>cicla</em>; SC), which is a more commonly consumed microgreen. Microgreens were grown in coconut coir irrigated at sowing with water containing different concentrations of sodium selenate (Na<sub>2</sub>SeO<sub>4</sub>; 0, 1, 3, and 5 mg Se L<sup>-1</sup>) or zinc sulphate (ZnSO<sub>4</sub>; 0, 2, 6, and 10 mg Zn L<sup>-1</sup>). The impact of adding a solution containing different concentrations of Se (0, 1, 3, and 5 mg L<sup>-1</sup> Se) or Zn (0, 2, 6, and 10 mg L<sup>-1</sup> Zn) to the substrate, at the time of sowing, on the growth and nutraceutical characteristics of the microgreens of both species was investigated together with their potential contribution to the daily requirements of Se and Zn for humans. The fresh weight and concentration of pigments, antioxidant compounds, especially ascorbic acid, and nitrate, and the total antioxidant capacity of microgreens were significantly higher in RO than in SC. Treatment with Se or Zn did not significantly affect the yield and quality of SC and RO microgreens, while increasing the microgreen concentration of Se (from 0.57 to 6.96 mg kg<sup>-1</sup> FW) or Zn (from 4.46 to 12.55 mg kg<sup>-1</sup> FW), respectively. Biofortification was more effective with Se than with Zn. A serving dose of 10 g of microgreens biofortified with 5 mg L<sup>-1</sup> of Se could meet the daily requirement of this element in humans. In order to have a significant input (15%) to the daily requirements of Zn from SC and RO microgreens obtained with 10 mg L<sup>-1</sup> Zn, the serving dose should be at least 100 gr.</div></div>","PeriodicalId":21679,"journal":{"name":"Scientia Horticulturae","volume":"338 ","pages":"Article 113668"},"PeriodicalIF":3.9,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142322960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-26DOI: 10.1016/j.scienta.2024.113672
Jiamin Jiang, Zilong Li, Yahui Cao, Yan Ding, Dingding Feng, Shuhong Ye
Endophytes are a group of microorganisms that exist in various tissues, and organs of plants and are not harmful to plants. In this study, we treated artificially injured 'Nanguo' pear with the endophyte Rhodococcus sp. FY-5 from Ginkgo biloba, and explored the effects of different treatments on the wound healing of 'Nanguo' pear by observing the population dynamics of the bacterial strains at the wound. The results showed that Ginkgo biloba endophyte Rhodococcus sp. FY-5 was able to stabilize the colonization at the fruit wounds, and the 'Nanguo' pear was treated with Ginkgo biloba endophyte Rhodococcus sp. FY-5 had higher activities of phenylalanine ammonia-lyase (PAL), 4-coumarate-CoA ligase (4CL), and cinnamate 4-hydroxylase (C4H), which promoted the accumulation of phenolic compounds, maintained high antioxidant activity, and activated the ascorbic acid-glutathione cycle to inhibit the mechanical damage-induced reactive oxygen species (ROS). In addition, the treatment of Ginkgo biloba endophyte Rhodococcus sp. FY-5 inhibited the activity of polyphenol oxidase (PPO), which effectively alleviated the browning response of 'Nanguo' pear after mechanical damage. In conclusion, the Ginkgo biloba endophyte Rhodococcus sp. FY-5 could promote the healing of 'Nanguo' pear wounds by regulating the phenylpropane metabolism and the necrotic acid-glutathione cycle in 'Nanguo' pear wounds.
{"title":"Ginkgo biloba endophyte Rhodococcus sp. FY-5 promotes wound healing in 'Nanguo' Pear by regulating the AsA-GSH cycle and phenylpropane metabolism","authors":"Jiamin Jiang, Zilong Li, Yahui Cao, Yan Ding, Dingding Feng, Shuhong Ye","doi":"10.1016/j.scienta.2024.113672","DOIUrl":"10.1016/j.scienta.2024.113672","url":null,"abstract":"<div><div>Endophytes are a group of microorganisms that exist in various tissues, and organs of plants and are not harmful to plants. In this study, we treated artificially injured 'Nanguo' pear with the endophyte <em>Rhodococcus</em> sp. FY-5 from Ginkgo biloba, and explored the effects of different treatments on the wound healing of 'Nanguo' pear by observing the population dynamics of the bacterial strains at the wound. The results showed that Ginkgo biloba endophyte <em>Rhodococcus</em> sp. FY-5 was able to stabilize the colonization at the fruit wounds, and the 'Nanguo' pear was treated with Ginkgo biloba endophyte <em>Rhodococcus</em> sp. FY-5 had higher activities of phenylalanine ammonia-lyase (PAL), 4-coumarate-CoA ligase (4CL), and cinnamate 4-hydroxylase (C4H), which promoted the accumulation of phenolic compounds, maintained high antioxidant activity, and activated the ascorbic acid-glutathione cycle to inhibit the mechanical damage-induced reactive oxygen species (ROS). In addition, the treatment of Ginkgo biloba endophyte <em>Rhodococcus</em> sp. FY-5 inhibited the activity of polyphenol oxidase (PPO), which effectively alleviated the browning response of 'Nanguo' pear after mechanical damage. In conclusion, the Ginkgo biloba endophyte <em>Rhodococcus</em> sp. FY-5 could promote the healing of 'Nanguo' pear wounds by regulating the phenylpropane metabolism and the necrotic acid-glutathione cycle in 'Nanguo' pear wounds.</div></div>","PeriodicalId":21679,"journal":{"name":"Scientia Horticulturae","volume":"338 ","pages":"Article 113672"},"PeriodicalIF":3.9,"publicationDate":"2024-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142322958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-25DOI: 10.1016/j.scienta.2024.113673
Xuan Zheng , Shuo Yang , Lu Chen, Xiao-Min Wang, Guo-Xin Cheng
The need for a lengthy maturation period is a key factor that reduces the market value of hot peppers (Capsicum annuum L.). A study was performed to assess the effects of the red (R)/blue (B)/white (W) qualities of light at reducing the period of maturation and changes in the metabolites of hot peppers. The use of the 4R:1B:5W group produced the optimal parameter of light qualities, which resulted in a higher percentage of red fruits, earlier seed development and a shorter maturation period. Additional research showed that the reduction in the period of maturation is closely related to the stage from seed to flowering owing to observation of the need for more days to complete the stage in all the groups. A combined analysis of the transcriptome-metabolome in the hot pepper flowers showed that the purple flowers in the 4R:1B:5W group were associated with an increase in the contents of flavonoids, particularly vitexin and cyanin, which are regulated by various metabolites, such as p-coumaroyl quinic acid, taxifolin, and quercetin among others. Increased levels of the total organic acids and phenolics, as shown by the decrease in the contents of total amino acids and carbohydrates also play a key role in the development of flowers in the 4R:1B:5W group. This may be closely related to the plant hormones induced by the quality of light. Ethylene responsive factors, particularly ERF021, were identified as potential hub regulators during the development of flowers owing to their involvement in all the associated metabolic pathways. This finding will provide key insights to improve the commercial value of hot peppers.
{"title":"Effect of different light emitting diode (LED) light quality parameters on the maturation period and development of flowers in hot pepper (Capsicum annuum L.)","authors":"Xuan Zheng , Shuo Yang , Lu Chen, Xiao-Min Wang, Guo-Xin Cheng","doi":"10.1016/j.scienta.2024.113673","DOIUrl":"10.1016/j.scienta.2024.113673","url":null,"abstract":"<div><div>The need for a lengthy maturation period is a key factor that reduces the market value of hot peppers (<em>Capsicum annuum</em> L.). A study was performed to assess the effects of the red (R)/blue (B)/white (W) qualities of light at reducing the period of maturation and changes in the metabolites of hot peppers. The use of the 4R:1B:5W group produced the optimal parameter of light qualities, which resulted in a higher percentage of red fruits, earlier seed development and a shorter maturation period. Additional research showed that the reduction in the period of maturation is closely related to the stage from seed to flowering owing to observation of the need for more days to complete the stage in all the groups. A combined analysis of the transcriptome-metabolome in the hot pepper flowers showed that the purple flowers in the 4R:1B:5W group were associated with an increase in the contents of flavonoids, particularly vitexin and cyanin, which are regulated by various metabolites, such as <em>p</em>-coumaroyl quinic acid, taxifolin, and quercetin among others. Increased levels of the total organic acids and phenolics, as shown by the decrease in the contents of total amino acids and carbohydrates also play a key role in the development of flowers in the 4R:1B:5W group. This may be closely related to the plant hormones induced by the quality of light. Ethylene responsive factors, particularly <em>ERF021</em>, were identified as potential hub regulators during the development of flowers owing to their involvement in all the associated metabolic pathways. This finding will provide key insights to improve the commercial value of hot peppers.</div></div>","PeriodicalId":21679,"journal":{"name":"Scientia Horticulturae","volume":"338 ","pages":"Article 113673"},"PeriodicalIF":3.9,"publicationDate":"2024-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142316137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The olive fruit fly, Bactrocera oleae (Rossi), is the most economically important pest of the olive tree; the fly prefers some cultivars over others, although the reason for this is still unclear. Current research results indicate that volatile compounds of the olive tree have a greater influence on the fly's attraction to a host plant than the physical parameters of the olive fruit. Therefore, the aim of the study was to investigate the relationship between the degree of the olive fruit fly infestation and the volatiles released by the olive leaves and fruits of three cultivars with similar physical fruit parameters, grown in the same olive grove. Lastovka, Frantoio and Leccino cultivars were studied, HS-SPME/GC–MS was performed to determine the profile of volatiles in leaves and fruits and to investigate the relationship between volatiles and the olive fruit fly infestation. Frantoio was the olive cultivar with the highest infestation by the olive fruit fly (39.66 %), followed by Leccino (24 %) and Lastovka (5 %). Among total number of volatiles identified in the study (55), more were detected in the fruits (37) than in the leaves (26). Saturated hydrocarbons and terpenes were the most abundant volatile groups in the fruits, while terpenes were found the most abundant in the leaves. The amounts of (E)-4,8-dimethylnona-1,3,7-triene and heneicosane were highest in the olive fruits of all cultivars tested. Heneicosane and eicosane were mainly released from ripe fruits and strongly correlated with the degree of infestation with the olive fruit fly. In addition, henicosane was the most abundant volatile compound in ripe fruits, along with 2,6-di-tert-butyl-4-methyl-phenol. Therefore, the results of the study suggest that fruit-derived volatiles have the greatest impact on the attraction of the olive fruit fly. The use of plant volatiles as attractants could be an effective tool for the sustainable control of olive trees to reduce the damage caused by the olive fruit fly while avoiding negative impacts on the environment and biodiversity.
{"title":"The relationship between the degree of infestation with Bactrocera oleae Rossi (Diptera: Tephritidae) and the volatiles released by the olive canopy","authors":"Elda Vitanović , Marijana Popović , Filipa Burul , Jakša Rošin , Slavko Perica , Mirella Žanetić , Katja Žanić , Maja Jukić Špika","doi":"10.1016/j.scienta.2024.113671","DOIUrl":"10.1016/j.scienta.2024.113671","url":null,"abstract":"<div><div>The olive fruit fly, <em>Bactrocera oleae</em> (Rossi), is the most economically important pest of the olive tree; the fly prefers some cultivars over others, although the reason for this is still unclear. Current research results indicate that volatile compounds of the olive tree have a greater influence on the fly's attraction to a host plant than the physical parameters of the olive fruit. Therefore, the aim of the study was to investigate the relationship between the degree of the olive fruit fly infestation and the volatiles released by the olive leaves and fruits of three cultivars with similar physical fruit parameters, grown in the same olive grove. Lastovka, Frantoio and Leccino cultivars were studied, HS-SPME/GC–MS was performed to determine the profile of volatiles in leaves and fruits and to investigate the relationship between volatiles and the olive fruit fly infestation. Frantoio was the olive cultivar with the highest infestation by the olive fruit fly (39.66 %), followed by Leccino (24 %) and Lastovka (5 %). Among total number of volatiles identified in the study (55), more were detected in the fruits (37) than in the leaves (26). Saturated hydrocarbons and terpenes were the most abundant volatile groups in the fruits, while terpenes were found the most abundant in the leaves. The amounts of (<em>E</em>)-4,8-dimethylnona-1,3,7-triene and heneicosane were highest in the olive fruits of all cultivars tested. Heneicosane and eicosane were mainly released from ripe fruits and strongly correlated with the degree of infestation with the olive fruit fly. In addition, henicosane was the most abundant volatile compound in ripe fruits, along with 2,6-di-tert-butyl-4-methyl-phenol. Therefore, the results of the study suggest that fruit-derived volatiles have the greatest impact on the attraction of the olive fruit fly. The use of plant volatiles as attractants could be an effective tool for the sustainable control of olive trees to reduce the damage caused by the olive fruit fly while avoiding negative impacts on the environment and biodiversity.</div></div>","PeriodicalId":21679,"journal":{"name":"Scientia Horticulturae","volume":"338 ","pages":"Article 113671"},"PeriodicalIF":3.9,"publicationDate":"2024-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142316049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In this study, postharvest behavioural dynamics among 52 diverse chilli genotypes was observed during storage (15°C) to discriminate and cluster them in accordance to their inherent responses. Fruit weight, size, weight loss, colour change, firmness, capsaicin, chlorophyll, carotenoids (red and yellow fractions), moisture content and purchase preference were recorded. Significant differences were observed among genotypes. A2 x IIVRC 18057 and IIVRC 18093 registered minimum (24.4 %) and maximum (71.7 %) weight loss, respectively at the end of storage. In all genotypes, moisture content declined with storage. Genotype dependent inverse relationship between capsaicin content at green and red stage was observed (r= -0.51). Firmness ranged between highest (4.65 N) in IIVRC 22100 on day zero, to lowest (0.14 N) in A3 x F5–112 on day 10. IIVRC 18131, Kashi Surkh, and IIVRC 18160 showed higher firmness. The loss of smooth glossy appearance, colour change and carotenoids accumulation showed variable responses during storage. Minimum colour changes were observed in IC119455, IC114957, IC119326, IC119327, EC 578666, IIVRC 18093, IIVRC 23002 and IIVRC 20005. Chlorophyll ranged between 68.63 µg/g fw (Pusa Jwala) to 270.61 µg/g fw (Kashi Gaurav) initially. Chemometric analysis using clustering and principal component analysis was performed. IIVRC 18093 and IIVRC 18253 were found most distant and can be utilized in breeding. IIVRC 18057 clearly indicated consumer liking in preference ranking test over other genotypes on final day. Overall, inherent diversity pointed the potential for careful selection of specific genotypes for definite breeding, consumer acceptability, marketing and storability strategies.
{"title":"Exploring changes in texture, colour, pungency and storability in diverse chilli genotypes for discriminating behaviour beyond harvest","authors":"Swati Sharma , Sudhir Singh , S.K. Singh , Rajesh Kumar , Indivar Prasad , Shreya Panwar , Hare Krishna , Anant Bahadur , Nagendra Rai","doi":"10.1016/j.scienta.2024.113669","DOIUrl":"10.1016/j.scienta.2024.113669","url":null,"abstract":"<div><div>In this study, postharvest behavioural dynamics among 52 diverse chilli genotypes was observed during storage (15°C) to discriminate and cluster them in accordance to their inherent responses. Fruit weight, size, weight loss, colour change, firmness, capsaicin, chlorophyll, carotenoids (red and yellow fractions), moisture content and purchase preference were recorded. Significant differences were observed among genotypes. A2 x IIVRC 18057 and IIVRC 18093 registered minimum (24.4 %) and maximum (71.7 %) weight loss, respectively at the end of storage. In all genotypes, moisture content declined with storage. Genotype dependent inverse relationship between capsaicin content at green and red stage was observed (<em>r</em>= -0.51). Firmness ranged between highest (4.65 N) in IIVRC 22100 on day zero, to lowest (0.14 N) in A3 x F5–112 on day 10. IIVRC 18131, Kashi Surkh, and IIVRC 18160 showed higher firmness. The loss of smooth glossy appearance, colour change and carotenoids accumulation showed variable responses during storage. Minimum colour changes were observed in IC119455, IC114957, IC119326, IC119327, EC 578666, IIVRC 18093, IIVRC 23002 and IIVRC 20005. Chlorophyll ranged between 68.63 µg/g fw (Pusa Jwala) to 270.61 µg/g fw (Kashi Gaurav) initially. Chemometric analysis using clustering and principal component analysis was performed. IIVRC 18093 and IIVRC 18253 were found most distant and can be utilized in breeding. IIVRC 18057 clearly indicated consumer liking in preference ranking test over other genotypes on final day. Overall, inherent diversity pointed the potential for careful selection of specific genotypes for definite breeding, consumer acceptability, marketing and storability strategies.</div></div>","PeriodicalId":21679,"journal":{"name":"Scientia Horticulturae","volume":"338 ","pages":"Article 113669"},"PeriodicalIF":3.9,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142316136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-24DOI: 10.1016/j.scienta.2024.113674
Shouxing Wen , Xiongjie Lin , Jingyi He , Ling-Yuan Zhang , Xianda Wang , Lijie Zhang , Yanjin Lin , Hanqing Hu , Jiahui Xu , Jing-Hao Huang
Female sterility is one of the keys determining seedlessness formation in citrus. However, the molecular basis for female sterility in citrus is still far from fully understood. In the present study, differentially expressed genes were identified in sexual organs of ‘Meiguicheng’ orange (Citrus sinensis) and its seedless mutant, candidate genes were then verified by RT-PCR, live-cell imaging technique and physiological biochemistry. Using high-throughput sequencing, 96 and 710 differentially expressed genes (DEGs) were captured in the ovaries and anthers, respectively, during megasporogenesis and microsporogenesis (Ph1). The number of DEGs in ovaries and anthers increased to 325 and 1760, respectively, post gametophyte abortion (Ph2). GO analysis and non-structural carbohydrates determination displayed that disturbances to starch and sucrose metabolism in anthers were responsible for male sterility. In Ph1 ovaries, five DEGs were enriched in reproduction process, one of which (Cs7g06410) was also involved in signaling. Whereas in Ph2 ovaries, eight DEGs were enriched in reproductive process, accompanied with four other DEGs involved in plant hormone signal transduction. PlantTFDB prediction indicated that, in the ovary, four transcription factors (TFs) at Ph1 were differentially expressed. Our findings displayed that Cs2g16620 (PIN1), Cs7g06410 (NPH3), and Cs3g23070 (MYB) might play pivotal roles in citrus female sterility through manipulating auxin transport and accumulation within the ovules, providing potential genes for further investigations on female gametophyte development and transgenic breeding in citrus.
{"title":"Comparative transcriptome analysis between ‘Meiguicheng’ (Citrus sinensis) and its seedless mutant during early sexual organs development","authors":"Shouxing Wen , Xiongjie Lin , Jingyi He , Ling-Yuan Zhang , Xianda Wang , Lijie Zhang , Yanjin Lin , Hanqing Hu , Jiahui Xu , Jing-Hao Huang","doi":"10.1016/j.scienta.2024.113674","DOIUrl":"10.1016/j.scienta.2024.113674","url":null,"abstract":"<div><div>Female sterility is one of the keys determining seedlessness formation in citrus. However, the molecular basis for female sterility in citrus is still far from fully understood. In the present study, differentially expressed genes were identified in sexual organs of ‘Meiguicheng’ orange (<em>Citrus sinensis</em>) and its seedless mutant, candidate genes were then verified by RT-PCR, live-cell imaging technique and physiological biochemistry. Using high-throughput sequencing, 96 and 710 differentially expressed genes (DEGs) were captured in the ovaries and anthers, respectively, during megasporogenesis and microsporogenesis (Ph1). The number of DEGs in ovaries and anthers increased to 325 and 1760, respectively, post gametophyte abortion (Ph2). GO analysis and non-structural carbohydrates determination displayed that disturbances to starch and sucrose metabolism in anthers were responsible for male sterility. In Ph1 ovaries, five DEGs were enriched in reproduction process, one of which (<em>Cs7g06410</em>) was also involved in signaling. Whereas in Ph2 ovaries, eight DEGs were enriched in reproductive process, accompanied with four other DEGs involved in plant hormone signal transduction. PlantTFDB prediction indicated that, in the ovary, four transcription factors (TFs) at Ph1 were differentially expressed. Our findings displayed that <em>Cs2g16620</em> (<em>PIN1</em>)<em>, Cs7g06410</em> (<em>NPH3</em>), and <em>Cs3g23070</em> (<em>MYB</em>) might play pivotal roles in citrus female sterility through manipulating auxin transport and accumulation within the ovules, providing potential genes for further investigations on female gametophyte development and transgenic breeding in citrus.</div></div>","PeriodicalId":21679,"journal":{"name":"Scientia Horticulturae","volume":"338 ","pages":"Article 113674"},"PeriodicalIF":3.9,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142316138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-24DOI: 10.1016/j.scienta.2024.113662
Shuming Nie, Ruozhu Zhao, Wenwen Yang, Jiajia Li, Dan Wang
Low temperatures can affect plant growth and geographical distribution. This study identified an MYB transcription factor, which is a positive regulator of chilling tolerance in tomato (Solanum lycopersicum), designated SlMYB1R1. The germination vigor and radicle lengths of overexpression SlMYB1R1 (SlMYB1R1OE) lines were significantly higher than those of the wild-type (Micro-Tom; MT) lines during germination. The leaf angle and leaf length of SlMYB1R1OE plants were significantly greater than those of MT plants. Furthermore, the expression of SlMYB1R1 was induced by cold stress. The degree of wilting was lower in SlMYB1R1OE plants than in MT plants under chilling stress. Overexpression of SlMYB1R1 in tomatoes resulted in low relative electrolyte leakage, malondialdehyde content, and reactive oxygen levels. The activities of peroxidase and catalase in SlMYB1R1OE plants were higher than those in MT plants after chilling stress. During cold stress, the transcriptional levels of the four cold-related genes were higher in SlMYB1R1OE lines than in MT plants. These results show that SlMYB1R1 plays key roles in tolerance to chilling stress in tomato.
{"title":"Overexpression of SlMYB1R1 improves chilling stress tolerance in tomato","authors":"Shuming Nie, Ruozhu Zhao, Wenwen Yang, Jiajia Li, Dan Wang","doi":"10.1016/j.scienta.2024.113662","DOIUrl":"10.1016/j.scienta.2024.113662","url":null,"abstract":"<div><div>Low temperatures can affect plant growth and geographical distribution. This study identified an MYB transcription factor, which is a positive regulator of chilling tolerance in tomato (<em>Solanum lycopersicum</em>), designated <em>SlMYB1R1</em>. The germination vigor and radicle lengths of overexpression <em>SlMYB1R1</em> (<em>SlMYB1R1OE</em>) lines were significantly higher than those of the wild-type (Micro-Tom; MT) lines during germination. The leaf angle and leaf length of <em>SlMYB1R1OE</em> plants were significantly greater than those of MT plants. Furthermore, the expression of <em>SlMYB1R1</em> was induced by cold stress. The degree of wilting was lower in <em>SlMYB1R1OE</em> plants than in MT plants under chilling stress. Overexpression of <em>SlMYB1R1</em> in tomatoes resulted in low relative electrolyte leakage, malondialdehyde content, and reactive oxygen levels. The activities of peroxidase and catalase in <em>SlMYB1R1OE</em> plants were higher than those in MT plants after chilling stress. During cold stress, the transcriptional levels of the four cold-related genes were higher in <em>SlMYB1R1OE</em> lines than in MT plants. These results show that <em>SlMYB1R1</em> plays key roles in tolerance to chilling stress in tomato.</div></div>","PeriodicalId":21679,"journal":{"name":"Scientia Horticulturae","volume":"338 ","pages":"Article 113662"},"PeriodicalIF":3.9,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142316048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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