In this study, postharvest behavioural dynamics among 52 diverse chilli genotypes was observed during storage (15°C) to discriminate and cluster them in accordance to their inherent responses. Fruit weight, size, weight loss, colour change, firmness, capsaicin, chlorophyll, carotenoids (red and yellow fractions), moisture content and purchase preference were recorded. Significant differences were observed among genotypes. A2 x IIVRC 18057 and IIVRC 18093 registered minimum (24.4 %) and maximum (71.7 %) weight loss, respectively at the end of storage. In all genotypes, moisture content declined with storage. Genotype dependent inverse relationship between capsaicin content at green and red stage was observed (r= -0.51). Firmness ranged between highest (4.65 N) in IIVRC 22100 on day zero, to lowest (0.14 N) in A3 x F5–112 on day 10. IIVRC 18131, Kashi Surkh, and IIVRC 18160 showed higher firmness. The loss of smooth glossy appearance, colour change and carotenoids accumulation showed variable responses during storage. Minimum colour changes were observed in IC119455, IC114957, IC119326, IC119327, EC 578666, IIVRC 18093, IIVRC 23002 and IIVRC 20005. Chlorophyll ranged between 68.63 µg/g fw (Pusa Jwala) to 270.61 µg/g fw (Kashi Gaurav) initially. Chemometric analysis using clustering and principal component analysis was performed. IIVRC 18093 and IIVRC 18253 were found most distant and can be utilized in breeding. IIVRC 18057 clearly indicated consumer liking in preference ranking test over other genotypes on final day. Overall, inherent diversity pointed the potential for careful selection of specific genotypes for definite breeding, consumer acceptability, marketing and storability strategies.
{"title":"Exploring changes in texture, colour, pungency and storability in diverse chilli genotypes for discriminating behaviour beyond harvest","authors":"Swati Sharma , Sudhir Singh , S.K. Singh , Rajesh Kumar , Indivar Prasad , Shreya Panwar , Hare Krishna , Anant Bahadur , Nagendra Rai","doi":"10.1016/j.scienta.2024.113669","DOIUrl":"10.1016/j.scienta.2024.113669","url":null,"abstract":"<div><div>In this study, postharvest behavioural dynamics among 52 diverse chilli genotypes was observed during storage (15°C) to discriminate and cluster them in accordance to their inherent responses. Fruit weight, size, weight loss, colour change, firmness, capsaicin, chlorophyll, carotenoids (red and yellow fractions), moisture content and purchase preference were recorded. Significant differences were observed among genotypes. A2 x IIVRC 18057 and IIVRC 18093 registered minimum (24.4 %) and maximum (71.7 %) weight loss, respectively at the end of storage. In all genotypes, moisture content declined with storage. Genotype dependent inverse relationship between capsaicin content at green and red stage was observed (<em>r</em>= -0.51). Firmness ranged between highest (4.65 N) in IIVRC 22100 on day zero, to lowest (0.14 N) in A3 x F5–112 on day 10. IIVRC 18131, Kashi Surkh, and IIVRC 18160 showed higher firmness. The loss of smooth glossy appearance, colour change and carotenoids accumulation showed variable responses during storage. Minimum colour changes were observed in IC119455, IC114957, IC119326, IC119327, EC 578666, IIVRC 18093, IIVRC 23002 and IIVRC 20005. Chlorophyll ranged between 68.63 µg/g fw (Pusa Jwala) to 270.61 µg/g fw (Kashi Gaurav) initially. Chemometric analysis using clustering and principal component analysis was performed. IIVRC 18093 and IIVRC 18253 were found most distant and can be utilized in breeding. IIVRC 18057 clearly indicated consumer liking in preference ranking test over other genotypes on final day. Overall, inherent diversity pointed the potential for careful selection of specific genotypes for definite breeding, consumer acceptability, marketing and storability strategies.</div></div>","PeriodicalId":21679,"journal":{"name":"Scientia Horticulturae","volume":"338 ","pages":"Article 113669"},"PeriodicalIF":3.9,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142316136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-24DOI: 10.1016/j.scienta.2024.113674
Shouxing Wen , Xiongjie Lin , Jingyi He , Ling-Yuan Zhang , Xianda Wang , Lijie Zhang , Yanjin Lin , Hanqing Hu , Jiahui Xu , Jing-Hao Huang
Female sterility is one of the keys determining seedlessness formation in citrus. However, the molecular basis for female sterility in citrus is still far from fully understood. In the present study, differentially expressed genes were identified in sexual organs of ‘Meiguicheng’ orange (Citrus sinensis) and its seedless mutant, candidate genes were then verified by RT-PCR, live-cell imaging technique and physiological biochemistry. Using high-throughput sequencing, 96 and 710 differentially expressed genes (DEGs) were captured in the ovaries and anthers, respectively, during megasporogenesis and microsporogenesis (Ph1). The number of DEGs in ovaries and anthers increased to 325 and 1760, respectively, post gametophyte abortion (Ph2). GO analysis and non-structural carbohydrates determination displayed that disturbances to starch and sucrose metabolism in anthers were responsible for male sterility. In Ph1 ovaries, five DEGs were enriched in reproduction process, one of which (Cs7g06410) was also involved in signaling. Whereas in Ph2 ovaries, eight DEGs were enriched in reproductive process, accompanied with four other DEGs involved in plant hormone signal transduction. PlantTFDB prediction indicated that, in the ovary, four transcription factors (TFs) at Ph1 were differentially expressed. Our findings displayed that Cs2g16620 (PIN1), Cs7g06410 (NPH3), and Cs3g23070 (MYB) might play pivotal roles in citrus female sterility through manipulating auxin transport and accumulation within the ovules, providing potential genes for further investigations on female gametophyte development and transgenic breeding in citrus.
{"title":"Comparative transcriptome analysis between ‘Meiguicheng’ (Citrus sinensis) and its seedless mutant during early sexual organs development","authors":"Shouxing Wen , Xiongjie Lin , Jingyi He , Ling-Yuan Zhang , Xianda Wang , Lijie Zhang , Yanjin Lin , Hanqing Hu , Jiahui Xu , Jing-Hao Huang","doi":"10.1016/j.scienta.2024.113674","DOIUrl":"10.1016/j.scienta.2024.113674","url":null,"abstract":"<div><div>Female sterility is one of the keys determining seedlessness formation in citrus. However, the molecular basis for female sterility in citrus is still far from fully understood. In the present study, differentially expressed genes were identified in sexual organs of ‘Meiguicheng’ orange (<em>Citrus sinensis</em>) and its seedless mutant, candidate genes were then verified by RT-PCR, live-cell imaging technique and physiological biochemistry. Using high-throughput sequencing, 96 and 710 differentially expressed genes (DEGs) were captured in the ovaries and anthers, respectively, during megasporogenesis and microsporogenesis (Ph1). The number of DEGs in ovaries and anthers increased to 325 and 1760, respectively, post gametophyte abortion (Ph2). GO analysis and non-structural carbohydrates determination displayed that disturbances to starch and sucrose metabolism in anthers were responsible for male sterility. In Ph1 ovaries, five DEGs were enriched in reproduction process, one of which (<em>Cs7g06410</em>) was also involved in signaling. Whereas in Ph2 ovaries, eight DEGs were enriched in reproductive process, accompanied with four other DEGs involved in plant hormone signal transduction. PlantTFDB prediction indicated that, in the ovary, four transcription factors (TFs) at Ph1 were differentially expressed. Our findings displayed that <em>Cs2g16620</em> (<em>PIN1</em>)<em>, Cs7g06410</em> (<em>NPH3</em>), and <em>Cs3g23070</em> (<em>MYB</em>) might play pivotal roles in citrus female sterility through manipulating auxin transport and accumulation within the ovules, providing potential genes for further investigations on female gametophyte development and transgenic breeding in citrus.</div></div>","PeriodicalId":21679,"journal":{"name":"Scientia Horticulturae","volume":"338 ","pages":"Article 113674"},"PeriodicalIF":3.9,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142316138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-24DOI: 10.1016/j.scienta.2024.113662
Shuming Nie, Ruozhu Zhao, Wenwen Yang, Jiajia Li, Dan Wang
Low temperatures can affect plant growth and geographical distribution. This study identified an MYB transcription factor, which is a positive regulator of chilling tolerance in tomato (Solanum lycopersicum), designated SlMYB1R1. The germination vigor and radicle lengths of overexpression SlMYB1R1 (SlMYB1R1OE) lines were significantly higher than those of the wild-type (Micro-Tom; MT) lines during germination. The leaf angle and leaf length of SlMYB1R1OE plants were significantly greater than those of MT plants. Furthermore, the expression of SlMYB1R1 was induced by cold stress. The degree of wilting was lower in SlMYB1R1OE plants than in MT plants under chilling stress. Overexpression of SlMYB1R1 in tomatoes resulted in low relative electrolyte leakage, malondialdehyde content, and reactive oxygen levels. The activities of peroxidase and catalase in SlMYB1R1OE plants were higher than those in MT plants after chilling stress. During cold stress, the transcriptional levels of the four cold-related genes were higher in SlMYB1R1OE lines than in MT plants. These results show that SlMYB1R1 plays key roles in tolerance to chilling stress in tomato.
{"title":"Overexpression of SlMYB1R1 improves chilling stress tolerance in tomato","authors":"Shuming Nie, Ruozhu Zhao, Wenwen Yang, Jiajia Li, Dan Wang","doi":"10.1016/j.scienta.2024.113662","DOIUrl":"10.1016/j.scienta.2024.113662","url":null,"abstract":"<div><div>Low temperatures can affect plant growth and geographical distribution. This study identified an MYB transcription factor, which is a positive regulator of chilling tolerance in tomato (<em>Solanum lycopersicum</em>), designated <em>SlMYB1R1</em>. The germination vigor and radicle lengths of overexpression <em>SlMYB1R1</em> (<em>SlMYB1R1OE</em>) lines were significantly higher than those of the wild-type (Micro-Tom; MT) lines during germination. The leaf angle and leaf length of <em>SlMYB1R1OE</em> plants were significantly greater than those of MT plants. Furthermore, the expression of <em>SlMYB1R1</em> was induced by cold stress. The degree of wilting was lower in <em>SlMYB1R1OE</em> plants than in MT plants under chilling stress. Overexpression of <em>SlMYB1R1</em> in tomatoes resulted in low relative electrolyte leakage, malondialdehyde content, and reactive oxygen levels. The activities of peroxidase and catalase in <em>SlMYB1R1OE</em> plants were higher than those in MT plants after chilling stress. During cold stress, the transcriptional levels of the four cold-related genes were higher in <em>SlMYB1R1OE</em> lines than in MT plants. These results show that <em>SlMYB1R1</em> plays key roles in tolerance to chilling stress in tomato.</div></div>","PeriodicalId":21679,"journal":{"name":"Scientia Horticulturae","volume":"338 ","pages":"Article 113662"},"PeriodicalIF":3.9,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142316048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-24DOI: 10.1016/j.scienta.2024.113663
Mohammad Shahid , Zaryab Shafi , Talat Ilyas , Udai B. Singh , John Pichtel
In conventional agricultural practices, pesticides are applied to protect crops from harmful insect pests; however, pervasive usage in high-yield crop systems poses a significant risk to the viability and sustainability of agroecosystems. Agricultural output may be adversely affected by pesticide deposition in the soil as it affects biochemical interactions between plants and soil. Pesticides cause oxidative stress by blocking physiological and biochemical pathways and disrupting the photosynthetic machinery of plants. When exposed to abiotic challenges, plant growth regulators (PGRs) such as auxin, gibberellins, cytokinin and abscisic acid (ABA), salicylic acid (SA), jasmonic acid (JA), brassinosteroids (BR), and 24-epibrassinolides (EBL) reduce pesticide toxicity by strengthening antioxidant defence mechanisms and enhancing tolerance to stressful conditions. By modulating a variety of physio-biochemical mechanisms, PGRs reduce pesticide toxicity in intact plants. Furthermore, PGRs eliminate reactive oxygen species (ROS) generation by inducing antioxidant enzyme production. Pesticide residues in plant compartments are reduced as a result of PGR-mediated increase in pesticide degradation. This review provides a detailed account of the potential role of PGRs in pesticide detoxification and growth promotion in plants. This work examines several elements of plant pesticidal reactions and assesses how PGRs support plants in tolerating pesticides. The underlying mechanisms during pesticide stress are also discussed. The need for additional study on PGR applications is also emphasized.
{"title":"Crosstalk between phytohormones and pesticides: Insights into unravelling the crucial roles of plant growth regulators in improving crop resilience to pesticide stress","authors":"Mohammad Shahid , Zaryab Shafi , Talat Ilyas , Udai B. Singh , John Pichtel","doi":"10.1016/j.scienta.2024.113663","DOIUrl":"10.1016/j.scienta.2024.113663","url":null,"abstract":"<div><div>In conventional agricultural practices, pesticides are applied to protect crops from harmful insect pests; however, pervasive usage in high-yield crop systems poses a significant risk to the viability and sustainability of agroecosystems. Agricultural output may be adversely affected by pesticide deposition in the soil as it affects biochemical interactions between plants and soil. Pesticides cause oxidative stress by blocking physiological and biochemical pathways and disrupting the photosynthetic machinery of plants. When exposed to abiotic challenges, plant growth regulators (PGRs) such as auxin, gibberellins, cytokinin and abscisic acid (ABA), salicylic acid (SA), jasmonic acid (JA), brassinosteroids (BR), and 24-epibrassinolides (EBL) reduce pesticide toxicity by strengthening antioxidant defence mechanisms and enhancing tolerance to stressful conditions. By modulating a variety of physio-biochemical mechanisms, PGRs reduce pesticide toxicity in intact plants. Furthermore, PGRs eliminate reactive oxygen species (ROS) generation by inducing antioxidant enzyme production. Pesticide residues in plant compartments are reduced as a result of PGR-mediated increase in pesticide degradation. This review provides a detailed account of the potential role of PGRs in pesticide detoxification and growth promotion in plants. This work examines several elements of plant pesticidal reactions and assesses how PGRs support plants in tolerating pesticides. The underlying mechanisms during pesticide stress are also discussed. The need for additional study on PGR applications is also emphasized.</div></div>","PeriodicalId":21679,"journal":{"name":"Scientia Horticulturae","volume":"338 ","pages":"Article 113663"},"PeriodicalIF":3.9,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142312160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-23DOI: 10.1016/j.scienta.2024.113666
Carolina Albuquerque Massena Ribeiro , Anderson Roberto Benedetti , Magda Andreia Tessmer , Nina Reis Soares , Líllian B. Januário Bibiano , Beatriz Appezzato da Gloria , Sonia M. De Stefano Piedade , Kaio Olímpio das Graças Dias , Maria Lucia Carneiro Vieira
Despite their economic significance, some tropical fruit species are largely neglected, mainly in terms of genetics and breeding. This is particularly true for the sweet passion fruit (Passiflora alata), a crop gaining new popularity and market value due to its characteristic aroma and flavor. Importantly, P. alata is self-incompatible, and the fruit set is reduced (or does not occur) when genetically related individuals are crossed. Given this, we determined the compatibility relationships among eight full sibs, previously selected. Nearly 2,000 crosses were performed in a full diallel design, including reciprocal and self-pollination. We propose that the genotypes’ reaction is a consequence of a sporophytic mechanism involved in P. alata self-incompatibility. Significantly, the compatibility results were confirmed in another field experiment conducted in the same environment. This allowed us to choose the genotypes to be intercrossed, resulting in 24 families. Next, nine traits related to fruit quality and yield were assessed in a randomized block design with four replicates, and plots consisting of three plants. Using genotypic values predicted by BLUP and two selection indices, eight superior families (30%) were selected. At the same time, the flowering period of each family was assessed. The families show a coincident flowering peak in May, except for the one family and its reciprocal, both with a flowering peak in November. Our findings indicate that compatibility tests and flowering phenology should be considered in P. alata breeding programs to make fruits available to producers for as long as possible.
{"title":"Self-incompatibility and phenotypic assessment of yield and quality in Passiflora alata, an understudied tropical fruit species","authors":"Carolina Albuquerque Massena Ribeiro , Anderson Roberto Benedetti , Magda Andreia Tessmer , Nina Reis Soares , Líllian B. Januário Bibiano , Beatriz Appezzato da Gloria , Sonia M. De Stefano Piedade , Kaio Olímpio das Graças Dias , Maria Lucia Carneiro Vieira","doi":"10.1016/j.scienta.2024.113666","DOIUrl":"10.1016/j.scienta.2024.113666","url":null,"abstract":"<div><div>Despite their economic significance, some tropical fruit species are largely neglected, mainly in terms of genetics and breeding. This is particularly true for the sweet passion fruit (<em>Passiflora alata</em>), a crop gaining new popularity and market value due to its characteristic aroma and flavor. Importantly, <em>P. alata</em> is self-incompatible, and the fruit set is reduced (or does not occur) when genetically related individuals are crossed. Given this, we determined the compatibility relationships among eight full sibs, previously selected. Nearly 2,000 crosses were performed in a full diallel design, including reciprocal and self-pollination. We propose that the genotypes’ reaction is a consequence of a sporophytic mechanism involved in <em>P. alata</em> self-incompatibility. Significantly, the compatibility results were confirmed in another field experiment conducted in the same environment. This allowed us to choose the genotypes to be intercrossed, resulting in 24 families. Next, nine traits related to fruit quality and yield were assessed in a randomized block design with four replicates, and plots consisting of three plants. Using genotypic values predicted by BLUP and two selection indices, eight superior families (30%) were selected. At the same time, the flowering period of each family was assessed. The families show a coincident flowering peak in May, except for the one family and its reciprocal, both with a flowering peak in November. Our findings indicate that compatibility tests and flowering phenology should be considered in <em>P. alata</em> breeding programs to make fruits available to producers for as long as possible.</div></div>","PeriodicalId":21679,"journal":{"name":"Scientia Horticulturae","volume":"338 ","pages":"Article 113666"},"PeriodicalIF":3.9,"publicationDate":"2024-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142312244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-23DOI: 10.1016/j.scienta.2024.113667
Yu-Xi Wan , Sheng-Min Liang , Qiang-Sheng Wu , Abeer Hashem , Elsayed Fathi Abd_Allah , Ying-Ning Zou
Chlorophylls are pivotal in capturing and converting light energy in leaves during photosynthesis, and changes in their biosynthetic pathway represent an adaptive response of plants to challenging environmental conditions. Serendipita indica, a culturable endophytic fungus, has demonstrated potential in enhancing the host plant's drought tolerance, whereas the underlying mechanisms (e.g., chlorophyll biosynthesis pathways) remain unclear. This study explored the impact of S. indica on the growth, chlorophyll synthesis intermediates and chlorophyll components, chlorophyll fluorescence parameters, photosynthesis, and the expression of chlorophyll synthesis-associated genes in trifoliate orange (Poncirus trifoliata) plants subjected to seven weeks of water deficit (WD). Following WD, the colonization of S. indica in roots significantly increased. Despite the growth-inhibiting effects of WD on trifoliate orange, inoculation with S. indica led to a substantial increase in aboveground growth performance and biomass production, with the magnitude of increase being more pronounced under WD than under no water dificit (NW). The WD treatment led to a reduction in the levels of leaf chlorophyll synthesis intermediates, while the colonization of S. indica significantly elevated the levels of 5-aminolevulimic acid, Mg-protoporphyrin IX, protoporphyrin IX, porphobilinogen, and protochlorophyllide in leaves, thus increasing chlorophyll a, b, and a + b concentrations in S. indica-treated plants, particularly under WD. S. indica inoculationi also up-regulated the expression level of PtHEMG1, PtCHLH, PtHEMA1, and PtHEME2 genes under WD. Interestingly, chlorophyll intermediates and the expression of chlorophyll-associated genes were significantly correlated with chlorophyll a in uninoculated plants and with chlorophyll b in inoculated plants. Under WD, S. indica-inoculated plants represented higher steady-state light quantum efficiency and lower steady-state nonphotochemical fluorescence quenching than uninoculated plants, which protected the photosynthetic apparatus from damage and increased the quantum efficiency of PSII. In addition, leaf gas exchange parameters were distinctly boosted by S. indica, with the increase being more pronounced under WD than under NW. In conclusion, S. indica-inoculated plants have better plant growth, chlorophyll biosynthesis, photosynthetic efficiency and gas exchange to adapt to WD.
叶绿素是叶片在光合作用过程中捕捉和转换光能的关键，叶绿素生物合成途径的变化代表了植物对挑战性环境条件的适应性反应。Serendipita indica 是一种可培养的内生真菌，在增强寄主植物的耐旱性方面具有潜力，但其潜在机制（如叶绿素生物合成途径）仍不清楚。本研究探讨了 S. indica 对缺水（WD）七周的三叶橙（Poncirus trifoliata）植株的生长、叶绿素合成中间体和叶绿素成分、叶绿素荧光参数、光合作用以及叶绿素合成相关基因表达的影响。缺水七周后，根部的蝙蝠蛾定植率明显增加。尽管缺水对三叶橙的生长有抑制作用，但接种 S. indica 后，三叶橙的地上部生长表现和生物量产量都有大幅提高，缺水时的提高幅度比不缺水（NW）时更明显。WD 处理导致叶片叶绿素合成中间体水平降低，而 S. indica 的定植则显著提高了叶片中 5-氨基乙酰丙酸、Mg-原卟啉 IX、原卟啉 IX、卟啉原和原叶绿素苷的水平，从而提高了 S. indica 处理植物的叶绿素 a、b 和 a + b 浓度，尤其是在 WD 条件下。在 WD 条件下，接种 S. indica 也会上调 PtHEMG1、PtCHLH、PtHEMA1 和 PtHEME2 基因的表达水平。有趣的是，叶绿素中间体和叶绿素相关基因的表达与未接种植株的叶绿素 a 和接种植株的叶绿素 b 显著相关。在 WD 条件下，接种 S. indica 的植株比未接种植株表现出更高的稳态光量子效率和更低的稳态非光化学荧光淬灭，这保护了光合装置免受损伤，提高了 PSII 的量子效率。此外，S. indica 对叶片气体交换参数也有明显的促进作用，在 WD 条件下的促进作用比在 NW 条件下更明显。总之，接种 S. indica 的植株在植物生长、叶绿素生物合成、光合效率和气体交换方面都能更好地适应 WD。
{"title":"Serendipita indica accelerates chlorophyll synthesis pathway and photosynthetic efficiency in trifoliate orange subjected to water deficit","authors":"Yu-Xi Wan , Sheng-Min Liang , Qiang-Sheng Wu , Abeer Hashem , Elsayed Fathi Abd_Allah , Ying-Ning Zou","doi":"10.1016/j.scienta.2024.113667","DOIUrl":"10.1016/j.scienta.2024.113667","url":null,"abstract":"<div><div>Chlorophylls are pivotal in capturing and converting light energy in leaves during photosynthesis, and changes in their biosynthetic pathway represent an adaptive response of plants to challenging environmental conditions. <em>Serendipita indica</em>, a culturable endophytic fungus, has demonstrated potential in enhancing the host plant's drought tolerance, whereas the underlying mechanisms (e.g., chlorophyll biosynthesis pathways) remain unclear. This study explored the impact of <em>S. indica</em> on the growth, chlorophyll synthesis intermediates and chlorophyll components, chlorophyll fluorescence parameters, photosynthesis, and the expression of chlorophyll synthesis-associated genes in trifoliate orange (<em>Poncirus trifoliata</em>) plants subjected to seven weeks of water deficit (WD). Following WD, the colonization of <em>S. indica</em> in roots significantly increased. Despite the growth-inhibiting effects of WD on trifoliate orange, inoculation with <em>S. indica</em> led to a substantial increase in aboveground growth performance and biomass production, with the magnitude of increase being more pronounced under WD than under no water dificit (NW). The WD treatment led to a reduction in the levels of leaf chlorophyll synthesis intermediates, while the colonization of <em>S. indica</em> significantly elevated the levels of 5-aminolevulimic acid, Mg-protoporphyrin IX, protoporphyrin IX, porphobilinogen, and protochlorophyllide in leaves, thus increasing chlorophyll <em>a, b</em>, and <em>a</em> + <em>b</em> concentrations in <em>S. indica</em>-treated plants, particularly under WD. <em>S. indica</em> inoculationi also up-regulated the expression level of <em>PtHEMG1, PtCHLH, PtHEMA1</em>, and <em>PtHEME2</em> genes under WD. Interestingly, chlorophyll intermediates and the expression of chlorophyll-associated genes were significantly correlated with chlorophyll <em>a</em> in uninoculated plants and with chlorophyll <em>b</em> in inoculated plants. Under WD, <em>S. indica</em>-inoculated plants represented higher steady-state light quantum efficiency and lower steady-state nonphotochemical fluorescence quenching than uninoculated plants, which protected the photosynthetic apparatus from damage and increased the quantum efficiency of PSII. In addition, leaf gas exchange parameters were distinctly boosted by <em>S. indica</em>, with the increase being more pronounced under WD than under NW. In conclusion, <em>S. indica</em>-inoculated plants have better plant growth, chlorophyll <em>b</em>iosynthesis, photosynthetic efficiency and gas exchange to adapt to WD.</div></div>","PeriodicalId":21679,"journal":{"name":"Scientia Horticulturae","volume":"338 ","pages":"Article 113667"},"PeriodicalIF":3.9,"publicationDate":"2024-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142312159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-22DOI: 10.1016/j.scienta.2024.113659
Ibandalin Mawlong , M. S. Sujith Kumar , Om Prakash Premi , Basant Kumar Kandpal , Bishal Gurung , Babli Mog , Reema Rani , Vanlalruati , Vijay Veer Singh
The study explores the effect of varying levels of nitrogen (N) application on N allocation in Indian mustard (Brassica juncea) genotypes from vegetative to the generative phases. The use of a heat map and PCA biplot analysis identifies two distinct sets of ideotypes. The first set is represented by genotype IC212031 having “Stay-green” phenotype with effective N regulating enzymes, high N uptake (NUpt) and better root development. The second set of ideotype is represented by HB9902 having delayed flowering phenology phases. The study highlights the relevance of Nitrate reductase (NR) in the final oil buildup in seeds (r = 0.5, p = 0.09). The considerable rise in NR activity from pre-anthesis to post-anthesis stages translates to higher seed storage compounds such as oil (r=−0.65, p = 0.03). And the developed prediction models confirmed the significance of these stages (pre-anthesis and post-anthesis) in the accumulation of seed storage components with emphasis on oil content. Similarly, this prediction model helps us to study the cause and effect relationship between various factors and oil content. The notable rise in Nitrite reductase (NiR) activity during the post-anthesis phase suggests its role in assimilating N for the storage of seed components, most likely proteins. Further, the inverse relationship between seed N (−0.53,p= 0.09) and oil content (−0.65, p= 0.03) confirmed that at the post anthesis stage the allocation of N towards precursor carbon compounds happens, which in turn forms either proteins or lipids. This becomes a critical factor in determining the final composition of the seed. Irrespective of N treatment and genotype, the N allocation follows the pattern: seed > siliquae husk > stover > root. However, higher N application rates primarily result in increased NUpt, but not necessarily improve utilisation. Overall, the study highlights the roles of root characteristics, N metabolising enzymes, and flowering phenology in enhancing N allocation and seed production in Indian mustard offering valuable insights for breeding strategies to enhance seed yield, oil content and nutrient efficiency in oilseed crops.
{"title":"Understanding nitrogen allocation dynamics in Indian mustard: Insights from enzyme activity and ideotype analysis","authors":"Ibandalin Mawlong , M. S. Sujith Kumar , Om Prakash Premi , Basant Kumar Kandpal , Bishal Gurung , Babli Mog , Reema Rani , Vanlalruati , Vijay Veer Singh","doi":"10.1016/j.scienta.2024.113659","DOIUrl":"10.1016/j.scienta.2024.113659","url":null,"abstract":"<div><div>The study explores the effect of varying levels of nitrogen (N) application on N allocation in Indian mustard (<em>Brassica juncea)</em> genotypes from vegetative to the generative phases. The use of a heat map and PCA biplot analysis identifies two distinct sets of ideotypes. The first set is represented by genotype IC212031 having “Stay-green” phenotype with effective N regulating enzymes, high N uptake (NUpt) and better root development. The second set of ideotype is represented by HB9902 having delayed flowering phenology phases. The study highlights the relevance of <em>Nitrate reductase</em> (NR) in the final oil buildup in seeds (<em>r</em> = 0.5, <em>p</em> = 0.09). The considerable rise in NR activity from pre-anthesis to post-anthesis stages translates to higher seed storage compounds such as oil (<em>r</em>=−0.65, <em>p</em> = 0.03). And the developed prediction models confirmed the significance of these stages (pre-anthesis and post-anthesis) in the accumulation of seed storage components with emphasis on oil content. Similarly, this prediction model helps us to study the cause and effect relationship between various factors and oil content. The notable rise in <em>Nitrite reductase</em> (NiR) activity during the post-anthesis phase suggests its role in assimilating N for the storage of seed components, most likely proteins. Further, the inverse relationship between seed N (−0.53,<em>p</em> <em>=</em> 0.09) and oil content (−0.65, <em>p</em> <em>=</em> 0.03) confirmed that at the post anthesis stage the allocation of N towards precursor carbon compounds happens, which in turn forms either proteins or lipids. This becomes a critical factor in determining the final composition of the seed. Irrespective of N treatment and genotype, the N allocation follows the pattern: seed > siliquae husk > stover > root. However, higher N application rates primarily result in increased NUpt, but not necessarily improve utilisation. Overall, the study highlights the roles of root characteristics, N metabolising enzymes, and flowering phenology in enhancing N allocation and seed production in Indian mustard offering valuable insights for breeding strategies to enhance seed yield, oil content and nutrient efficiency in oilseed crops.</div></div>","PeriodicalId":21679,"journal":{"name":"Scientia Horticulturae","volume":"338 ","pages":"Article 113659"},"PeriodicalIF":3.9,"publicationDate":"2024-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142275665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-21DOI: 10.1016/j.scienta.2024.113622
Shuangxia Luo , Qiang Li , Yifan Kang , Yuhang Liu , Xing Wang , Ping Yu , Jiajia Shen , Lijun Song , Zhishuo Zhang , Xueping Chen
Male sterility (MS) is a desirable tool for hybrid seed production. Eggplant is one of the most widely grown fruit vegetables all over the world. Although a lot of male-sterile lines were created in eggplant, the genes underlying the MS regulation in eggplant remain largely unknown. EMS (ethyl methane sulfonate)- mutagenized mutant is an ideal material for the identification of genes regulating MS in eggplant. In the present study, we identified a mutant, named smms1 (Solanum melongena male sterility 1) with smaller flowers and abnormal anthers. Further analyses indicated the pollens in smms1 showed highly vacuolated and significant decreased number, size and viability compared to wild type (WT). Bulked segregant analysis sequencing (BSA-seq) and Mutmap analyses identified a G-to-A mutation in an alternative splicing region in the 2nd intron of Smechr0501647. Smechr0501647 encodes a subunit of Mediator (MED) that functions as a bridge between transcription factors and RNA polymerase II. The SNP results in three splicing events with 11 bp and 15 bp deletion in the 2nd exon and the retention of the 1st intron. Notably, the events with 11 bp deletion and intron retention led to premature stop codons. Gene Ontology (GO) enrichment of Differentially expressed gene (DEG) suggested that while up-regulated DEGs in smms1 are enriched in phytohormone related processes, the enriched GO terms of down-regulated DEGs were mainly related to cellular processes. The measurement of the phytohormone contents in WT and smms1 indicated the involvement of the abscisic acid (ABA), indole-3-acetic acid (IAA), salicylic acid (SA), jasmonic acid (JA) and cytokinins (CKs) in the MS regulation mediated by SmMS1 in eggplant. The discovery of the candidate gene controlling MS not only provides a potential target for the molecular breeding of MS germplasms, but also provides an effective basis of for studying regulatory mechanisms of MS in eggplant.
雄性不育（MS）是杂交种子生产的理想工具。茄子是全世界种植最广泛的果菜之一。虽然在茄子中培育出了许多雄性不育系，但茄子中调控雄性不育的基因在很大程度上仍然未知。EMS（甲烷磺酸乙酯）诱变突变体是鉴定茄子 MS 调控基因的理想材料。在本研究中，我们发现了一个花朵较小且花药异常的突变体，命名为 smms1（Solanum melongena male sterility 1）。进一步的分析表明，与野生型（WT）相比，smms1 的花粉高度空泡化，数量、大小和活力显著下降。大量分离分析测序（BSA-seq）和突变图（Mutmap）分析确定了 Smechr0501647 第 2 个内含子中一个替代剪接区的 G 到 A 突变。Smechr0501647 编码 Mediator (MED) 的一个亚基，该亚基在转录因子和 RNA 聚合酶 II 之间起桥梁作用。该 SNP 导致三个剪接事件，其中第 2 个外显子缺失 11 bp 和 15 bp，第 1 个内含子保留。值得注意的是，11 bp 的缺失和内含子的保留会导致过早的终止密码子。差异表达基因（DEG）的基因本体（GO）富集表明，smms1 中上调的 DEGs 富集于与植物激素相关的过程，而下调的 DEGs 富集的 GO 术语主要与细胞过程有关。对 WT 和 smms1 植物激素含量的测定表明，脱落酸（ABA）、吲哚-3-乙酸（IAA）、水杨酸（SA）、茉莉酸（JA）和细胞分裂素（CKs）参与了 SmMS1 对茄子 MS 的调控。控制MS的候选基因的发现不仅为MS种质的分子育种提供了潜在的目标，也为研究茄子MS的调控机制提供了有效的依据。
{"title":"Mapping the candidate gene controlling male sterility using EMS-mutagenized mutant smms1 in eggplant (Solanum melongena L.)","authors":"Shuangxia Luo , Qiang Li , Yifan Kang , Yuhang Liu , Xing Wang , Ping Yu , Jiajia Shen , Lijun Song , Zhishuo Zhang , Xueping Chen","doi":"10.1016/j.scienta.2024.113622","DOIUrl":"10.1016/j.scienta.2024.113622","url":null,"abstract":"<div><div>Male sterility (MS) is a desirable tool for hybrid seed production. Eggplant is one of the most widely grown fruit vegetables all over the world. Although a lot of male-sterile lines were created in eggplant, the genes underlying the MS regulation in eggplant remain largely unknown. EMS (ethyl methane sulfonate)- mutagenized mutant is an ideal material for the identification of genes regulating MS in eggplant. In the present study, we identified a mutant, named <em>smms1</em> (<em>Solanum melongena male sterility 1</em>) with smaller flowers and abnormal anthers. Further analyses indicated the pollens in <em>smms1</em> showed highly vacuolated and significant decreased number, size and viability compared to wild type (WT). Bulked segregant analysis sequencing (BSA-seq) and Mutmap analyses identified a G-to-A mutation in an alternative splicing region in the 2nd intron of <em>Smechr0501647. Smechr0501647</em> encodes a subunit of Mediator (MED) that functions as a bridge between transcription factors and RNA polymerase II. The SNP results in three splicing events with 11 bp and 15 bp deletion in the 2nd exon and the retention of the 1st intron. Notably, the events with 11 bp deletion and intron retention led to premature stop codons. Gene Ontology (GO) enrichment of Differentially expressed gene (DEG) suggested that while up-regulated DEGs in <em>smms1</em> are enriched in phytohormone related processes, the enriched GO terms of down-regulated DEGs were mainly related to cellular processes. The measurement of the phytohormone contents in WT and <em>smms1</em> indicated the involvement of the abscisic acid (ABA), indole-3-acetic acid (IAA), salicylic acid (SA), jasmonic acid (JA) and cytokinins (CKs) in the MS regulation mediated by SmMS1 in eggplant. The discovery of the candidate gene controlling MS not only provides a potential target for the molecular breeding of MS germplasms, but also provides an effective basis of for studying regulatory mechanisms of MS in eggplant.</div></div>","PeriodicalId":21679,"journal":{"name":"Scientia Horticulturae","volume":"338 ","pages":"Article 113622"},"PeriodicalIF":3.9,"publicationDate":"2024-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0304423824007751/pdfft?md5=1f04652c466ecc71277acd1a9a3176f4&pid=1-s2.0-S0304423824007751-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142275666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-20DOI: 10.1016/j.scienta.2024.113665
Yue Zhang , Hui Zhi , Liuqing Qu , Dehu Su , Jianrang Luo
As one of the significant ornamental characteristics of tree peony, floral fragrance has garnered considerable attention. In this study, 65 cultivars of tree peony were classified into three groups based on their sensory evaluation scores: strong fragrance, medium fragrance, and no/light fragrance and 9 strong fragrance cultivars, 6 medium fragrance cultivars, and 2 light/no fragrance cultivars were selected for substance analysis. Using gas chromatography-mass spectrometry (GC–MS), 77 volatile organic compounds (VOCs) were identified and classified from 17 tree peony cultivars, they were terpenoids, benzenoids/phenylpropanoids, and fatty acid derivatives. Monoterpenoids were the main volatile compounds in the cultivars ‘Souvenir de Maxime Cornu’, ‘Okan’, ‘Chromatella’, ‘Yapianzi’, and ‘Liuli Guanzhu’, while benzenoids/phenylpropanoids were the main volatile compounds in ‘Shaonvqun’ and ‘Binglingzi’. Through the calculation of odor activity value (OAV), 17 cultivars were categorized into four distinct groups: flower-sweet scent, flower-rose scent, herb scent and mixed scent. This investigation presented the composition and classification of fragrant compounds of 17 tree peony cultivars and classified the odor profiles based on the contribution of flower volatiles to the overall olfactory experience, offering valuable materials for genetic enhancement of tree peony floral fragrance.
花香作为树牡丹的重要观赏特征之一，一直备受关注。本研究根据感官评价得分将 65 个树牡丹栽培品种分为三组：浓香型、中等香型和无/淡香型，并选择了 9 个浓香型栽培品种、6 个中等香型栽培品种和 2 个淡香/无香型栽培品种进行物质分析。利用气相色谱-质谱法（GC-MS）对 17 个牡丹品种中的 77 种挥发性有机化合物（VOCs）进行了鉴定和分类，它们分别是萜类化合物、苯类化合物/苯丙类化合物和脂肪酸衍生物。单萜是'Souvenir de Maxime Cornu'、'Okan'、'Chromatella'、'Yapianzi'和'Liuli Guanzhu'等品种的主要挥发性化合物，而苯并类/苯丙类则是'Shaonvqun'和'Binglingzi'等品种的主要挥发性化合物。通过气味活性值（OAV）的计算，17 个栽培品种被分为四类：花甜香型、花玫瑰香型、草本香型和混合香型。这项研究展示了 17 个树牡丹栽培品种的芳香化合物组成和分类，并根据花香挥发物对整体嗅觉体验的贡献对气味特征进行了分类，为树牡丹花香的遗传改良提供了宝贵的材料。
{"title":"Analysis of flower volatile compounds and odor classification of 17 tree peony cultivars","authors":"Yue Zhang , Hui Zhi , Liuqing Qu , Dehu Su , Jianrang Luo","doi":"10.1016/j.scienta.2024.113665","DOIUrl":"10.1016/j.scienta.2024.113665","url":null,"abstract":"<div><p>As one of the significant ornamental characteristics of tree peony, floral fragrance has garnered considerable attention. In this study, 65 cultivars of tree peony were classified into three groups based on their sensory evaluation scores: strong fragrance, medium fragrance, and no/light fragrance and 9 strong fragrance cultivars, 6 medium fragrance cultivars, and 2 light/no fragrance cultivars were selected for substance analysis. Using gas chromatography-mass spectrometry (GC–MS), 77 volatile organic compounds (VOCs) were identified and classified from 17 tree peony cultivars, they were terpenoids, benzenoids/phenylpropanoids, and fatty acid derivatives. Monoterpenoids were the main volatile compounds in the cultivars ‘Souvenir de Maxime Cornu’, ‘Okan’, ‘Chromatella’, ‘Yapianzi’, and ‘Liuli Guanzhu’, while benzenoids/phenylpropanoids were the main volatile compounds in ‘Shaonvqun’ and ‘Binglingzi’. Through the calculation of odor activity value (OAV), 17 cultivars were categorized into four distinct groups: flower-sweet scent, flower-rose scent, herb scent and mixed scent. This investigation presented the composition and classification of fragrant compounds of 17 tree peony cultivars and classified the odor profiles based on the contribution of flower volatiles to the overall olfactory experience, offering valuable materials for genetic enhancement of tree peony floral fragrance.</p></div>","PeriodicalId":21679,"journal":{"name":"Scientia Horticulturae","volume":"338 ","pages":"Article 113665"},"PeriodicalIF":3.9,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142271604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-20DOI: 10.1016/j.scienta.2024.113664
Ya Liu , Shuya Wang , Xiao Liu, Ruoman Wang, Weijie Chen, Jinwei Suo, Jingwei Yan, Jiasheng Wu
Agrobacterium-mediated transient gene expression is a powerful tool for characterizing gene function in plants, particularly in fruit trees with long juvenile periods. However, few studies have systematically explored transient expression in nut trees, such as T. grandis. In this study, we developed a rapid and easily manipulated transient transformation system using Agrobacterium-mediated injection infiltration in T. grandis cones. Cones at the enlargement stage (30 days after aril protrusion) were used, and the bacterial solution was gently injected into the kernel at a depth of 1 cm, filling nearly the entire cone, as indicated by a red dye. Using the green fluorescent protein (GFP) gene as a reporter for Agrobacterium-mediated transformation, we confirmed GFP expression in T. grandis cone successfully through confocal microscopy, western blot, and PCR. Transformation conditions, including bacterial density and days after agroinfiltration, were optimized for higher transformation efficiency, with the optimal conditions being an OD600 of 0.6 and an agroinfiltration period of 6 - 9 days. Transient overexpression of TgWRKY47, a cell size regulator, altered cell size in T. grandis cones, indicating that this Agrobacterium infiltration method can be used for gene functional analysis. Furthermore, confocal microscopy revealed that TgWRKY47-GFP was localized in the nucleus along with a nuclear marker, demonstrating its applicability for protein subcellular localization. In summary, this method presents a versatile tool for efficient and targeted transient gene functional analysis and subcellular localization in T. grandis cones, offering new insights into nut tree biology research.
{"title":"Agrobacterium-mediated transient expression in Torreya grandis cones: A simple and rapid tool for gene expression and functional gene assay","authors":"Ya Liu , Shuya Wang , Xiao Liu, Ruoman Wang, Weijie Chen, Jinwei Suo, Jingwei Yan, Jiasheng Wu","doi":"10.1016/j.scienta.2024.113664","DOIUrl":"10.1016/j.scienta.2024.113664","url":null,"abstract":"<div><p><em>Agrobacterium</em>-mediated transient gene expression is a powerful tool for characterizing gene function in plants, particularly in fruit trees with long juvenile periods. However, few studies have systematically explored transient expression in nut trees, such as <em>T. grandis</em>. In this study, we developed a rapid and easily manipulated transient transformation system using <em>Agrobacterium</em>-mediated injection infiltration in <em>T. grandis</em> cones. Cones at the enlargement stage (30 days after aril protrusion) were used, and the bacterial solution was gently injected into the kernel at a depth of 1 cm, filling nearly the entire cone, as indicated by a red dye. Using the <em>green fluorescent protein</em> (<em>GFP</em>) gene as a reporter for <em>Agrobacterium</em>-mediated transformation, we confirmed GFP expression in <em>T. grandis</em> cone successfully through confocal microscopy, western blot, and PCR. Transformation conditions, including bacterial density and days after agroinfiltration, were optimized for higher transformation efficiency, with the optimal conditions being an OD<sub>600</sub> of 0.6 and an agroinfiltration period of 6 - 9 days. Transient overexpression of <em>TgWRKY47</em>, a cell size regulator, altered cell size in <em>T. grandis</em> cones, indicating that this <em>Agrobacterium</em> infiltration method can be used for gene functional analysis. Furthermore, confocal microscopy revealed that TgWRKY47-GFP was localized in the nucleus along with a nuclear marker, demonstrating its applicability for protein subcellular localization. In summary, this method presents a versatile tool for efficient and targeted transient gene functional analysis and subcellular localization in <em>T. grandis</em> cones, offering new insights into nut tree biology research.</p></div>","PeriodicalId":21679,"journal":{"name":"Scientia Horticulturae","volume":"338 ","pages":"Article 113664"},"PeriodicalIF":3.9,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142271603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
扫码关注我们
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号