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Short communication: Zizyphus fruit fly (Carpomya incompleta (Becker), Diptera: Tephritidae) is expanding its range in Europe 短通讯:齐泽蝇(Carpomya incomplete (Becker),双翅目:蝗科)正在欧洲扩大其活动范围
IF 0.9 4区 农林科学 Q3 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2022-10-27 DOI: 10.5424/sjar/2022204-18961
I. Garrido-Jurado, E. Quesada-Moraga, M. Yousef-Yousef
Aim of study: To identify a new pest of jujube reported by the farmers in Spain. Area of study: The Iberian Peninsula (Spain). Material and methods: The insects were identified according to the mitochondrial cytochrome C oxidase subunit I (COI) gene and the main morphological features of this tephritid species, including the ocellar seta, the mesonotum and apical crossband in winds. Main results: Based on morphological characterization the insects were identified as Carpomya incompleta (Becker) (Diptera: Tephritidae), which was confirmed by the phylogenetic analysis with more than 94% of identity. Besides, the distance analysis showed very low intraspecific divergence in C. incompleta sequences. Research highlights: We report the presence of the zizyphus fruit fly in Spain for the first time. This presence has been reported to the Early Warning Systems on Alien Invasive Species of the Andalusian Government (Spain).
研究目的:鉴定西班牙农民报告的一种新的红枣害虫。研究领域:伊比利亚半岛(西班牙)。材料和方法:根据线粒体细胞色素C氧化酶亚基I(COI)基因和该种鞘翅目昆虫的主要形态特征,包括角刚毛、中孔和顶端横带,对昆虫进行鉴定。主要结果:根据形态特征,将这些昆虫鉴定为不完全卡氏菌(Carpomya incompletea,Becker)(Diptera:Tephritidae),系统发育分析证实其具有94%以上的同一性。此外,距离分析表明,不完全C.序列的种内差异非常小。研究重点:我们报道了西班牙首次出现的紫蝇。这种存在已向安达卢西亚政府的外来入侵物种预警系统报告(西班牙)。
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引用次数: 0
Analysis and evaluation of a dynamic model for greenhouse lettuce growth 温室莴苣生长动态模型的分析与评价
IF 0.9 4区 农林科学 Q3 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2022-10-20 DOI: 10.5424/sjar/2022204-18658
Chuyun Tan, Shanhong Zhang, Yu Guo, Yang Wang
Aim of study: We analyzed and evaluated a nonlinear dynamic crop growth model called NICOLET B3, which can predict the dry and fresh matter content of lettuce in greenhouses. Area of study: Calibration was performed using experimental data obtained from the literature. The experiment was carried out in Saltillo, Mexico, and in a greenhouse in Beijing, China. Material and methods: We identified and discussed the feasibility of the studied model with multi-dimensional evaluation criteria. Meanwhile, a sensitivity analysis of input variables was conducted. After that, the least square identification method was used to calibrate the most sensitive parameter values to improve the robustness of the model. Main results: Results demonstrate that: i) the NICOLET B3 model is able to predict the fresh and dry matter production of lettuce with satisfactory accuracy verified (R2 = 0.9939 for fresh matter and R2 = 0.9858 for dry matter); ii) temperature has the most obvious impact on the model performance, compared with photosynthetically active radiation and CO2 concentration; iii) the model could perform well with only two inputs. Research highlights: Simulation results of evaluated NICOLET B3 model have a perfect goodness-of-fit. A method of calibrating parameters of the model and sensitivity analysis of three input variables of the model can facilitate its application.
研究目的:对预测温室生菜干、鲜物质含量的非线性动态作物生长模型NICOLET B3进行了分析和评价。研究领域:使用从文献中获得的实验数据进行校准。该实验分别在墨西哥萨尔提略和中国北京的一个温室中进行。材料与方法:采用多维评价标准对研究模型的可行性进行了鉴定和探讨。同时,对输入变量进行了敏感性分析。然后,利用最小二乘辨识法对最敏感的参数值进行标定,提高模型的鲁棒性。主要结果:结果表明:i) NICOLET B3模型能够预测生菜鲜物质和干物质产量,准确度验证满意(鲜物质R2 = 0.9939,干物质R2 = 0.9858);ii)与光合有效辐射和CO2浓度相比,温度对模型性能的影响最为明显;Iii)该模型可以在只有两个输入的情况下表现良好。研究重点:评估的NICOLET B3模型仿真结果具有很好的拟合优度。对模型参数进行标定,并对模型的三个输入变量进行灵敏度分析,有利于模型的应用。
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引用次数: 0
Spatial discounting in food products from high natural value agroecosystems 高自然价值农业生态系统中粮食产品的空间贴现
IF 0.9 4区 农林科学 Q3 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2022-10-19 DOI: 10.5424/sjar/2022204-18480
Rubén Granado-Díaz, A. J. Villanueva, J. A. Gómez‐Limón
Aim of study: To test the hypothesis that consumers’ willingness to pay (WTP) for traditional food products expresses their willingness to conserve the bundle of ecosystem services (ES) provided by the territories of origin and that the intensity of these preferences is subject to spatial discounting. Area of study: We used Iberian dry-cured ham as a case study. This is a traditional and highly appreciated product characteristically produced in the Dehesa agroforestry system (southern Iberian Peninsula), an agricultural system characterized by high levels of ES provision. Material and methods: The analysis relies on a discrete choice experiment using some recently developed spatial indexes that go beyond traditional “distance-decay” effects. This method was fed with primary data gathered from a face-to-face survey administered in Andalusian food retail establishments to 1,158 respondents who had bought Iberian ham (acorn- or fodder-fed) at least once in the last year. Main results: The results provide evidence of the effects of spatial discounting on the purchase of acorn- and fodder-fed Iberian hams associated with the agroecosystem in which they are produced. These effects presumably stem from consumers’ cultural identity linked to the agroecosystem of origin and their willingness to support the local economy and communities. In addition, in the case of acorn-fed Iberian ham, spatial discounting is affected by consumers’ perception of the ES provided by the agroecosystem, with consumers who significantly perceive these services showing a higher WTP, regardless of their place of residence (no spatial discounting). Research highlights: Relevant insights can be gained from the results, especially concerning marketing strategies and the adoption of environmental and sociocultural certifications.
研究目的:检验消费者对传统食品的支付意愿表达了他们保护原产地提供的生态系统服务的意愿,以及这些偏好的强度受到空间折扣的影响的假设。研究领域:我们使用伊比利亚干腌火腿作为案例研究。这是德赫萨农林系统(伊比利亚半岛南部)生产的一种传统且备受赞赏的产品,该农业系统以高水平的ES供应为特点。材料和方法:该分析依赖于离散选择实验,使用了一些最近开发的超越传统“距离衰减”效应的空间指数。该方法采用了在安达卢西亚食品零售机构对1158名去年至少购买过一次伊比利亚火腿(橡子或饲料喂养)的受访者进行的面对面调查中收集的主要数据。主要结果:这些结果提供了空间折扣对购买橡子和饲料喂养的伊比利亚火腿的影响的证据,这些火腿与生产它们的农业生态系统有关。这些影响可能源于消费者与原产地农业生态系统相关的文化认同,以及他们支持当地经济和社区的意愿。此外,在橡子喂养的伊比利亚火腿的情况下,空间折扣受到消费者对农业生态系统提供的ES的感知的影响,显著感知这些服务的消费者表现出更高的WTP,无论他们的居住地如何(没有空间折扣)。研究亮点:可以从结果中获得相关见解,特别是关于营销策略以及环境和社会文化认证的采用。
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引用次数: 0
Prospective study of the technology for evaluating and measuring in-row seed spacing for precision planting: A review 精密种植行间距评价与测量技术的前瞻性研究
IF 0.9 4区 农林科学 Q3 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2022-10-10 DOI: 10.5424/sjar/2022204-19269
G. Nardon, G. Botta
Corn is the most cultivated and consumed cereal in the world. The overall objective of this review was to study the methodologies to measure and evaluate the in-row seed spacing for precision planting as well as to determine the technological alternatives that would allow obtaining information about seed mapping for corn crop planting in precision agriculture applications. As a conceptual synthesis about the electronic measurement system, there are two strategies for determining in-row seed spacing in the precision planting. Indirect methods correspond to the measurement before the seeds reach the furrow, while direct methods correspond to the measurement with the seeds placed in the furrow. The indirect measurement strategy is the most widely used in research publications and commercial planter monitors. Within this method, the seed spacing measurement systems use optical or radio wave type seed sensors. Corn seed counting accuracy through electronic measurement systems with optical-type seed sensor is at least 96%. The microwave seed sensor is used commercially by a few companies whose technologies are patented. The direct measurement strategy is under development and requires further research. The main limitation of these technologies is the seed detection in the furrow, which limits the planter travel speed and the equipment cost. The conceptual proposal for the term ‘seed mapping’ is to provide integrated and geo-referenced information on in-row seed spacing and depth for precision planting.
玉米是世界上种植和消费最多的谷物。本综述的总体目标是研究测量和评估精确种植的行内种子间距的方法,并确定技术替代方案,以获得有关精确农业应用中玉米作物种植的种子测绘信息。作为电子测量系统的概念综合,在精确种植中有两种确定行内种子间距的策略。间接方法对应于种子到达犁沟之前的测量,而直接方法对应于将种子放入犁沟中的测量。间接测量策略在研究出版物和商业播种机监控器中使用最为广泛。在该方法中,种子间距测量系统使用光学或无线电波类型的种子传感器。通过带有光学型种子传感器的电子测量系统,玉米种子计数的准确率至少为96%。微波种子传感器被一些技术获得专利的公司商业化使用。直接测量策略正在制定中,需要进一步研究。这些技术的主要局限性在于犁沟中的种子检测,这限制了播种机的行进速度和设备成本。“种子测绘”一词的概念建议是提供关于行内种子间距和深度的综合和地理参考信息,用于精确种植。
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引用次数: 1
Short communication: Effect of combined fungicide treatments on fatty acid content in Eisenia fetida earthworms 短通讯:杀菌剂联合处理对肥Eisenia fetida蚯蚓脂肪酸含量的影响
IF 0.9 4区 农林科学 Q3 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2022-10-10 DOI: 10.5424/sjar/2022204-19509
S. Khyzhnyak, S. Midyk, Sergei V. Polishchuk, A. O. Velinska
Aim of study: To study the toxic effects of a combined fungicide in Eisenia fetida earthworms and in its lipid fatty acid composition. Material and methods: We investigated the acute toxicity (LC50, 14 days) of a combined fungicide (active ingredients: carbendazim + cyproconazole) in Eisenia fetida using artificial substrate. The quantitative content of fatty acids (FAs) of body lipids was determined by the method of high-sensitivity gas chromatography. Main results: Moderate toxicity of the combined fungicide was determined for Eisenia fetida earthworms. Changes in behavioral response, biomass loss, and mortality of test objects were detected. A decrease in the content of saturated FAs was found. At the same time, an increase of long-chain polyunsaturated FAs content of ω6 and ω3 families, which are involved in the regulation of a wide range of physiological processes, was revealed. Modulation of the lipid FAs profile in Eisenia fetida worms is explained by the participation of the FAs in the restructuring of the organism reactivity system under fungicidal load. Research highlights: The reorganization of the FA profile can be considered as an early criterion of metabolic perturbations in earthworms under the action of fungicides. Understanding the toxic potential of fungicides to organisms in soil ecosystems is essential for practical risk assessment in response to pesticide application.
研究目的:研究复方杀菌剂对赤子爱胜蚯蚓的毒性作用及其脂质脂肪酸组成。材料与方法:采用人工基质,研究了多菌灵+环康唑复合杀菌剂对赤子爱胜酵母的急性毒性(LC50,14d)。采用高灵敏度气相色谱法测定血脂中脂肪酸的含量。主要结果:复方杀菌剂对赤子爱胜蚯蚓具有中等毒性。检测受试者的行为反应、生物量损失和死亡率的变化。发现饱和脂肪酸的含量降低。同时,ω6和ω3家族的长链多不饱和脂肪酸含量增加,这两个家族参与了一系列生理过程的调节。在杀真菌负荷下,脂肪脂肪酸参与生物体反应系统的重组,解释了铁爱胜虫脂质脂肪酸谱的调节。研究重点:FA谱的重组可以被认为是蚯蚓在杀菌剂作用下代谢紊乱的早期标准。了解杀菌剂对土壤生态系统中的生物的毒性潜力对于应对农药施用的实际风险评估至关重要。
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引用次数: 0
Early detection of graft-incompatibility in hawthorn (Crataegus azarolus L.) trees on apple, pear, and quince rootstocks 山楂树对苹果、梨和榅桲砧木嫁接不亲和性的早期检测
IF 0.9 4区 农林科学 Q3 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2022-10-10 DOI: 10.5424/sjar/2022204-19567
E. Kacal, O. Caliskan, Gökhan Ozturk, I. Gur, H. Kocal, Omer F. Karamursel, Derya Kilic, M. Moreno
Aim of study: This study was conducted to determine the usability of some clonal rootstocks of apple (MM 106 and MM 111), pear (Fox 11), and quince (Quince A) for hawthorn trees propagation. Area of study: Fruit Research Institute, Isparta and Hatay Mustafa Kemal University, Hatay, Turkey. Material and methods: ‘Sultan’ hawthorn cultivar was budded on the following clonal rootstocks: pear Fox 11, quince A, and apple MM 106 and MM 111. Plants of hawthorn seedlings (Crataegus azarolus L.) budded with ‘Sultan’ cv. were used as control. External visual diagnosis of the scion-rootstock graft combinations was performed by observing visual symptoms in budded trees growing in nursery conditions, as well as anatomic and histological investigations of the incompatibility in the laboratory. Main results: In this study, healthy scion development and callus tissue in the graft region were formed in the seedling rootstock as well as on Fox11 and Quince A clonal rootstocks. In contrast, MM 106 and MM 111 apple rootstocks showed incompatibility symptoms, with insufficient scion vigor and unstructured callus tissue development. Research highlights: ‘Tanslocated’ and ‘located’ graft incompatibility symptoms were observed in Sultan/MM 106 and Sultan/MM 111 combinations. Further studies are necessary to confirm the early good compatibility found in nursery conditions, testing the effect of those clonal rootstocks from different species (Fox11 and Quince A) on vigor, yield, and fruit quality traits in orchard conditions.
研究目的:研究苹果(mm106和mm111)、梨(Fox 11)和榅桲(quince A)的无性系砧木在山楂树繁殖中的可用性。研究领域:水果研究所,伊斯帕塔和哈塔伊穆斯塔法·凯末尔大学,哈塔伊,土耳其。材料和方法:‘苏丹’山楂品种在梨狐11号、榅桲A和苹果mm106和mm111克隆砧木上发芽。山楂幼苗植物(山楂山楂)发芽与'苏丹' cv。作为对照。通过在苗圃条件下观察发芽树的视觉症状,以及在实验室对不亲和性进行解剖和组织学调查,对接穗-砧木嫁接组合进行外部视觉诊断。主要结果:在幼苗砧木、Fox11和Quince A克隆砧木上均能形成健康的接穗发育和嫁接区愈伤组织。而mm106和mm111则表现出不亲和症状,接穗活力不足,愈伤组织发育不完整。研究重点:在Sultan/ mm106和Sultan/ mm111组合中观察到“移位”和“定位”移植物不相容症状。为了证实早期苗圃条件下的良好亲和性,需要进一步研究不同品种(Fox11和Quince A)的无性系砧木在果园条件下对活力、产量和果实品质性状的影响。
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引用次数: 0
The frequency of spring harvest and row distance affect the seed and forage production of alfalfa (Medicago sativa L.) under irrigated Mediterranean conditions 春收频次和行距对地中海灌溉条件下紫花苜蓿种子和饲料产量有影响
IF 0.9 4区 农林科学 Q3 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2022-10-03 DOI: 10.5424/sjar/2022204-18093
C. Chocarro, Á. Maresma, J. Lloveras
Aim of study: The effect of the number of spring harvests (1 or 2) for forage before letting alfalfa (Medicago sativa L.) grow for seed, combined with three different row distances, on seed production was evaluated over a three-year period. Area of study: Irrigated Mediterranean environment (NE Spain). Material and methods: Seed yield, forage production and seed weight were evaluated, as well as final plant stands and the economic implications of different decisions. Main results: The study revealed significant differences (p < 0.01) in seed yield between the number of forage harvests prior to letting the crop grow for seed in two of the three years of the study, with an overall average of 500 kg ha-1 for one spring harvest and 450 kg ha-1 for two spring harvests, but with significant differences associated with row distance in two of the three years. The average annual production of forage was 8.08 Mg of dry matter (DM) ha-1 for the case of two spring harvests and 7.57 Mg for one, with significant differences in one of the three years. Row distances of 20, 40 and 60 cm did not significantly affect forage DM production although, on average, higher forage yields were achieved with narrow row distances. From an economic point of view, our results suggest that the 1-harvest treatment before letting alfalfa grow for seed is slightly more profitable than the 2-harvest treatment (higher gross margin of about 54.5 € ha-1). Research highlights: Under irrigated Mediterranean conditions, alfalfa harvested only once in spring before letting the crop grow for seed production maximizes the gross margin.
研究目的:在三年的时间里,评估了苜蓿(Medicago sativa L.)生长种子前春季收获次数(1或2次)以及三种不同的行距对种子产量的影响。研究领域:地中海灌溉环境(西班牙东北部)。材料和方法:评估种子产量、饲料产量和种子重量,以及最终的林分和不同决策的经济影响。主要结果:该研究显示,在研究的三年中,有两年在让作物长出种子之前,牧草收获次数之间的种子产量存在显著差异(p<0.01),一次春收的总平均值为500 kg ha-1,两次春收为450 kg ha-1。但在三年中的两年中,与行距相关的差异存在显著性。两次春收的牧草年平均产量为8.08 Mg干物质(DM)ha-1,一次为7.57 Mg,三年中有一年差异显著。20、40和60cm的行距对饲料DM产量没有显著影响,尽管平均而言,窄行距可以获得更高的饲料产量。从经济角度来看,我们的研究结果表明,在让苜蓿生长成种子之前,一次收获处理的利润略高于两次收获处理(更高的毛利率约为54.5€ha-1)。研究重点:在地中海灌溉条件下,苜蓿在春季只收获一次,然后让作物生长用于种子生产,从而最大限度地提高了毛利率。
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引用次数: 0
Novel draught resistance sensing elements for measurement of drawbar power of agricultural machinery 农业机械牵引力测量用新型抗旱感测元件
IF 0.9 4区 农林科学 Q3 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2022-10-03 DOI: 10.5424/sjar/2022204-19171
Ganesh Upadhyay, H. Raheman, R. Dubey
Aim of study: To develop an instrumentation system comprising three force sensing elements to measure the draught resistance of any tillage and seeding tools during field operation by connecting one sensing element to each three-point linkage of the tractor. Area of study: Department of AgFE, Indian Institute of Technology, Kharagpur, India Material and methods: Commercial S-type transducers were packed laterally in between the curved plates perpendicular to the direction of travel in such a way that the magnitude of the imposed force decreased and its nature got reversed consequently during tillage force measurement. Finite element analysis was also performed on the proposed model of the sensing elements. The performance was evaluated on the basis of non-linearity, hysteresis, and non-repeatability. The data were validated with the draught values simultaneously recorded using instrumented three-point linkages of tractor. Main results: It offered good sensitivity and linearity during static calibration. The measurement capacity based on maximum applied load during static calibration was 10 kN with accuracy 93.40%. The low values of mean percentage error (9.03%), maximum absolute variation (17.43%), and root mean square error (0.51 kN) revealed good accuracy of the system. Validation was conducted by comparing the data for an offset type disk harrow with the model outputs of previous studies to assess its suitability for other soil working conditions, and the results were satisfactory. Research highlights: The advantages of this sensing device in the measurement of drawbar power are fewer changes in the hitching geometry, lower cost, and capability of quick hitching.
研究目的:开发一个由三个力传感元件组成的仪器系统,通过将一个传感元件连接到拖拉机的每个三点联动装置上,来测量田间作业期间任何耕作和播种工具的抗旱性。研究领域:印度理工学院AgFE系,Kharagpur,印度材料和方法:商业s型传感器横向包装在垂直于移动方向的弯曲板之间,这样在耕作力测量过程中施加的力的大小减小,其性质反过来。并对所提出的传感元件模型进行了有限元分析。基于非线性、迟滞和不可重复性对其性能进行了评价。数据与使用拖拉机仪表三点连杆同时记录的吃水值进行了验证。主要结果:该方法具有良好的灵敏度和线性度。静态校准时基于最大外加载荷的测量能力为10 kN,精度为93.40%。平均百分比误差(9.03%)、最大绝对变异(17.43%)和均方根误差(0.51 kN)均较低,表明系统具有较好的精度。通过将偏移式盘耙的数据与以往研究的模型输出进行对比验证,评估其对其他土壤工况的适用性,结果令人满意。研究重点:该传感装置在牵引杆功率测量中的优点是牵引杆几何形状变化少、成本低、能够快速牵引杆。
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引用次数: 6
Influence of D and Rec strains of plum pox virus on phenolic profile and antioxidant capacity of fresh plum fruits of ‘Čačanska Lepotica’ cultivar 李痘病毒D株和Rec株对“Čačanska Lepotica”品种新鲜李果实酚类物质和抗氧化能力的影响
IF 0.9 4区 农林科学 Q3 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2022-09-27 DOI: 10.5424/sjar/2022204-18179
N. Miletić, D. Jevremović, M. Mitić, B. Popović, M. Petković
Aim of study: To investigate the changes in chemical composition of fresh plum fruits cv. ‘Čačanska Lepotica’ prompted by the presence of various strains of plum pox virus (PPV). Area of study: Serbia Material and methods: In an experimental orchard of ‘Čačanska Lepotica’ plum cultivar, fruits were picked from virus-free and PPV-infected trees (PPV-D and PPV-Rec strains) in four harvest stages in 2017 and 2018. Fruits were further analyzed on total phenolics, flavonoids, anthocyanins, antioxidant capacity and selected phenolics. Main results: The results indicate that virus infection causes chemical changes to a certain extent, but mostly in initial harvest stages, while the values are equal in later stages. In the last harvest stage, as the most utilizable in commercial purposes, only chlorogenic acid content was affected in 2017, while in 2018 contents of neochlorogenic acid and chrysanthemin were altered by the PPV infection. Total contents of flavonoids and phenolics revealed no influence of virus infection during both 2017 and 2018, while PPV-Rec infected samples were richer in anthocyanins under heavy rainfall during summer months of 2018. Given the number of identified compounds (10) and the vast experimental data, it might be concluded that influence of PPV infection on chemical composition of ‘Čačanska Lepotica’ plum fruits was quite limited. Research highlights: Plum cultivar ‘Čačanska Lepotica’ should be considered as highly tolerant cultivar to PPV, and can be grown in heavily infected environment with no risk. Therefore, it might be a great replacement for sensitive plum cultivars, such as ‘Požegača’ and ‘Čačanska Rodna’.
研究目的:研究不同株李痘病毒(PPV)对鲜李化学成分的影响。研究领域:塞尔维亚材料和方法:在“Čačanska Lepotica”李品种的实验果园中,在2017年和2018年的四个收获阶段,从无病毒和PPV感染的树木(PPV-D和PPV-Rec菌株)中摘下果实。进一步分析了果实的总酚类、黄酮类、花青素、抗氧化能力和所选酚类物质。主要结果:结果表明,病毒感染在一定程度上会引起化学变化,但主要发生在收获初期,而在收获后期,这些值是相等的。在最后一个收获阶段,作为最可用于商业目的的作物,2017年只有绿原酸含量受到影响,而2018年PPV感染改变了新绿原酸和菊花素的含量。2017年和2018年,类黄酮和酚类物质的总含量均未受到病毒感染的影响,而2018年夏季强降雨下,PPV Rec感染的样品富含花青素。鉴于已鉴定化合物的数量(10)和大量的实验数据,可以得出结论,PPV感染对“Čačanska Lepotica”李子化学成分的影响非常有限。研究重点:李品种“Čačanska Lepotica”应被视为对PPV具有高度耐受性的品种,可以在感染严重的环境中生长,没有风险。因此,它可能是敏感李品种的绝佳替代品,如“Požegača”和“Čačanska Rodna”。
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引用次数: 0
Metarhizium anisopliae and Isaria fumosorosea challenge the survival and immunity of the palm weevil, Rhynchophorus ferrugineus Olivier 金龟子绿僵菌(Metarhizium anisopliae)和烟熏伊萨里亚(Isaria fummosorosea)对棕榈象鼻虫(Rhynchophorus ferrugineus Olivier)的生存和免疫构成挑战
IF 0.9 4区 农林科学 Q3 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2022-08-05 DOI: 10.5424/sjar/2022203-18979
R. Ahmed, S. Freed, A. Naeem, M. Akmal, C. Dietrich
Aim of study: Rhynchophorus ferrugineus Olivier is an invasive pest of palm worldwide. The use of insecticides by farmers for its management has been found insignificant. This study evaluated the potential use of entomopathogenic fungi for R. ferrugineus management with a particular focus on the fungal infection on the activities of different detoxification enzymes. Area of study: Grubs and adults of R. ferrugineus were collected from various infested date palm fields in the four provinces of Pakistan. Material and methods: Fungi Isaria fumosorosea (If-02) and Metarhizium anisopliae (Ma-M2) were evaluated against R. ferrugineus, and its immune responses were biochemically characterized. Main results: The highest mortality rate was recorded at concentration 3×108 spores mL-1 on the 7th day post infection in the populations treated with M. anisopliae from Punjab, Khyber Pakhtunkhwa (KPK), Sindh and Baluchistan (93.75, 90.0, 90.0 and 81.25% respectively). M. anisopliae with lowest LC50 (1.1×106 spores mL-1) from Sindh also proved to be the most lethal fungus against R. ferrugineus. Maximum acetylcholinesterase (AChE) and glutathione S-transferases (GSTs) activities were observed in Baluchistan (26.28 and 24.0 μmol min-1 mg-1 protein, respectively) and maximum esterases (EST) activity (35.4 μmol min-1 mg-1 protein) was observed in the KPK population on the 3rd-day post I. fumosorosea infection. Research highlights: Fungal infection by I. fumosorosea caused a significant increase in AChE, GST and EST activities which may hinder R. ferrugineus development. However, M. anisopliae, to some extent, also inhibited enzyme activities and yielded a sudden increase in mortality. Future bio-pesticides could be developed for integrated pest management (IPM) of palm weevil.
研究目的:Rhynchophorusferrogineus Olivier是一种入侵性棕榈害虫。农民使用杀虫剂进行管理的情况已被发现微不足道。本研究评估了昆虫病原真菌在铁芽孢菌管理中的潜在用途,特别关注真菌感染对不同解毒酶活性的影响。研究区域:从巴基斯坦四个省的各种受感染的椰枣田中采集了铁叶棕的Grub和成虫。材料和方法:对烟曲霉(Isaria fumosorosea,If-02)和绿僵菌(Metarhizium anisopliae,Ma-M2。主要结果:在旁遮普省、开伯尔-普赫图赫瓦省、信德省和俾路支省用3×108孢子mL-1处理的群体中,感染后第7天的死亡率最高(分别为93.75%、90.0、90.0和81.25%)。来自信德省的绿脓杆菌LC50最低(1.1×。俾路支省的乙酰胆碱酯酶(AChE)和谷胱甘肽S-转移酶(GSTs)活性最高(分别为26.28和24.0μmol min-1 mg-1蛋白),在烟曲霉感染后第3天,KPK人群的酯酶(EST)活性最高,为35.4μmol min-1mg-1蛋白。研究重点:烟曲霉的真菌感染导致AChE、GST和EST活性显著增加,可能阻碍铁芽孢的发育。然而,M.anisopliae在一定程度上也抑制了酶的活性,并导致死亡率突然增加。未来可以开发用于棕榈象甲害虫综合治理的生物农药。
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引用次数: 1
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Spanish Journal of Agricultural Research
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