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Ultrastructural observation on matrix fibers in the condylar cartilage of the adult rat mandible. 成年大鼠下颌骨髁突软骨基质纤维的超微结构观察。
S Shibata, O Baba, M Ohsako, S Suzuki, Y Yamashita, T Ichijo

In this study, ultrastructural and immunohistochemical studies were performed on the adult rat (15 weeks old) mandibular condyle, with particular attention to the matrix fibers in the condylar cartilage. The fibrous zone had thick collagen fibrils which formed fibril bundles. These collagen fibrils consisted mainly of Type I collagen. From the proliferative zone to the mature zone, the density of the collagen fibrils became higher. In the hypertrophic zone, thick collagen fibrils were formed around the chondrocytes. Immunohistochemical study indicated that these collagen fibrils consisted mainly of Type I collagen. Therefore, it was confirmed that the hypertrophic chondrocytes in this tissue had one of the osteoblastic phenotypes.

在本研究中,对成年大鼠(15周龄)下颌髁突进行了超微结构和免疫组织化学研究,特别关注髁突软骨中的基质纤维。纤维带有厚的胶原原纤维,形成纤维束。这些胶原原纤维主要由I型胶原组成。从增生区到成熟区,胶原原纤维密度增高。在增生性区,软骨细胞周围形成厚的胶原原纤维。免疫组织化学研究表明,这些胶原原纤维主要由I型胶原组成。因此,证实该组织中的增生性软骨细胞具有成骨细胞表型之一。
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引用次数: 0
Abnormal particle size of lipoprotein in non-insulin-dependent diabetics and nondiabetics with and without hyperlipidemia. 非胰岛素依赖型糖尿病患者和伴有或不伴有高脂血症的非糖尿病患者的脂蛋白颗粒大小异常。
Y Nakajo, A Tanaka, I Uchimura

To clarify the mechanism of the high incidence of ischemic heart disease in the diabetics, we studied the particle size of the lipoprotein with particular attention to the structural abnormality. Using high performance liquid chromatography, the particle size of the lipoprotein was determined with elution volume, which was precisely correlated with the particle size. The particle size of low density lipoprotein (LDL) from the diabetics with normolipidemia is larger than that from the nondiabetics (p less than 0.001) in the elution pattern of triglyceride. The examination of the elution pattern of the triglyceride and cholesterol revealed that this large LDL was composed of a large amount of triglyceride. These characteristics of the large LDL resembled that of the atherogenic intermediate density lipoprotein. The particle size of high density lipoprotein (HDL) from the diabetics with normolipidemia was larger than that from the nondiabetic controls (p less than 0.001), detected by the elution pattern of triglyceride. The comparison of the triglyceride and cholesterol elution pattern indicated that also the large HDL in the diabetics with normolipidemia was rich with triglyceride, which was reported to inhibit the activity of the lipid transfer protein. These facts revealed that the disorder of the lipid metabolism not accompanied with hyperlipidemia produced the large LDL and HDL, which might lead to the excess mortality rate of the ischemic heart disease in the diabetics even with normolipidemia.

为了阐明糖尿病患者缺血性心脏病高发的机制,我们研究了脂蛋白的粒径,特别关注其结构异常。采用高效液相色谱法,用洗脱体积测定脂蛋白的粒径,洗脱体积与颗粒大小精确相关。在甘油三酯洗脱模式中,降脂糖尿病患者的低密度脂蛋白(LDL)粒径大于非糖尿病患者(p < 0.001)。对甘油三酯和胆固醇洗脱模式的检查显示,这个大的LDL是由大量的甘油三酯组成的。大LDL的这些特征与致动脉粥样硬化的中密度脂蛋白相似。通过甘油三酯的洗脱模式检测,正常血脂的糖尿病患者的高密度脂蛋白(HDL)的粒径大于非糖尿病对照组(p < 0.001)。甘油三酯和胆固醇洗脱模式的比较表明,正常血脂的糖尿病患者的大HDL也富含甘油三酯,据报道,甘油三酯可以抑制脂转运蛋白的活性。这些事实表明,不伴有高脂血症的脂质代谢紊乱产生了大量的LDL和HDL,这可能导致糖尿病患者在正常血脂的情况下缺血性心脏病的死亡率过高。
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引用次数: 0
New restoration and direct pulp capping systems using adhesive composite resin. 新的修复和直接纸浆封盖系统使用粘合剂复合树脂。
T Kashiwada, M Takagi

There have been many arguments on the irritating mechanisms of the composite resin on the dental pulp. While the direct irritative effect of the resin has been preferred, some authors considered that the marginal microleakage and the resulting bacterial infection play a more important role in inducing the complicating pulp irritation. We developed a new filling technique, called the direct inlay restoration method, which could prevent the marginal leakage associated with the polymerization shrinkage of the adhesive composite resin. In this study, we tried to apply our method clinically. None of the 440 cases which were filled with the adhesive composite resin and 60 cases out of 64 cases in which the pulps were directly capped with the adhesive composite resin developed any signs and symptoms of pulp irritation. The other 4 cases developed signs of pulp irritation. Two of those 4 cases were pulpectomized due to spontaneous pain and the other 2 cases turned out to be well after re-restoration. With the informed consent of the patients, the direct pulp capping using the adhesive composite resin was experimentally performed on 6 caries-free 3rd molars and the histopathological examination of these capped molars revealed that neither significant degenerative nor inflammatory changes were brought about in the dental pulp. These clinical and histopathological observation suggest that the dental pulp irritation after resin filling is not induced by the composite resin itself.

关于复合树脂对牙髓的刺激机理,一直存在许多争论。虽然树脂的直接刺激作用是首选的,但一些作者认为边缘微渗漏和由此产生的细菌感染在诱发复合牙髓刺激方面起着更重要的作用。我们开发了一种新的填充技术,称为直接嵌体修复法,可以防止粘接复合树脂因聚合收缩而产生的边缘泄漏。在本研究中,我们尝试将我们的方法应用于临床。440例用黏附复合树脂充填的病例和64例直接用黏附复合树脂封盖的病例中60例均未出现牙髓刺激的迹象和症状。其余4例出现牙髓刺激征象。其中2例因自发性疼痛切除牙髓,2例术后恢复良好。在患者知情同意的情况下,我们对6颗无龋的第三磨牙进行了直接盖牙术,盖牙后的组织病理学检查显示牙髓未发生明显的退行性变化和炎症变化。这些临床和组织病理学观察表明,树脂充填后的牙髓刺激不是由复合树脂本身引起的。
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引用次数: 0
Transferrin receptor expression in oral tumors. 转铁蛋白受体在口腔肿瘤中的表达。
Pub Date : 1991-09-01 DOI: 10.11480/BTMD.380301
N. Tanaka, T. Miyamoto, M. Saito, T. Amagasa, S. Shioda, Y. Eishi, K. Hsieh
Transferrin receptor expression in oral tumors was examined by staining with monoclonal antibody against the human transferrin receptor. The cells with positive reaction were recognized in the basal and parabasal layers of the normal epithelium. The staining was found in all the malignant tumors but not in the benign tumors. These results suggest that the immunohistochemical analysis of the transferrin receptor is useful for the diagnosis of oral malignant tumor in addition to the clinical and pathological examinations.
用人转铁蛋白受体单克隆抗体染色检测口腔肿瘤组织中转铁蛋白受体的表达。阳性反应的细胞分布在正常上皮的基底层和准基底层。恶性肿瘤均可见染色,良性肿瘤未见染色。提示转铁蛋白受体的免疫组化分析除临床和病理检查外,对口腔恶性肿瘤的诊断有重要意义。
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引用次数: 10
Study on cholesteryl ester transfer activity in coronary heart disease. 冠心病中胆固醇酯转移活性的研究。
Y Fujinuma, A Tanaka, H Maezawa

The net cholesterol transfer activity from high density lipoprotein (HDL) to low density lipoprotein (LDL) was determined in the patients with coronary heart disease (CHD) to examine its effect on the pathogenesis of arteriosclerosis. Furthermore, in the CHD patients with high HDL cholesterolemia (more than 60 mg/dl), the HDL particle size was measured by high performance liquid chromatography. A significant cholesteryl ester transfer activity (P less than 0.02) was noted in the CHD patients with low HDL cholesterolemia (less than 60 mg/dl). The rate of cholesteryl ester transfer activity (cholesteryl ester transfer activity/hour) inversely correlated with the serum HDL cholesterol value (r = -0.483, P = 0.096) in the patients with CHD. These results suggest that an increase of CETA caused a low HDL cholesterol value in the CHD patients with low HDL cholesterolemia and it may have the risk of causing CHD. However, an increase of the CETA was not found in the CHD patients with high HDL cholesterolemia compared to the normal subjects, the HDL particle size being significantly greater than that in the normal subjects. In the CHD patients with high HDL cholesterolemia, the large size of HDL may have the risk of causing CHD.

测定冠心病(CHD)患者高密度脂蛋白(HDL)向低密度脂蛋白(LDL)的净胆固醇转移活性,探讨其在动脉硬化发病机制中的作用。此外,在高HDL胆固醇血症(大于60 mg/dl)的冠心病患者中,采用高效液相色谱法测量HDL颗粒大小。低HDL胆固醇血症(低于60 mg/dl)的冠心病患者胆固醇酯转移活性显著(P < 0.02)。冠心病患者胆固醇酯转移活性率(胆固醇酯转移活性/小时)与血清高密度脂蛋白胆固醇值呈负相关(r = -0.483, P = 0.096)。上述结果提示,CETA升高导致低HDL胆固醇血症的冠心病患者HDL胆固醇值降低,可能具有引起冠心病的风险。而高HDL胆固醇血症的冠心病患者与正常人相比,CETA并未增加,HDL颗粒大小明显大于正常人。在高HDL胆固醇血症的冠心病患者中,高密度脂蛋白的大小可能有引起冠心病的危险。
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引用次数: 0
Transferrin receptor expression in oral tumors. 转铁蛋白受体在口腔肿瘤中的表达。
N Tanaka, T Miyamoto, M Saito, T Amagasa, S Shioda, Y Eishi, K J Hsieh

Transferrin receptor expression in oral tumors was examined by staining with monoclonal antibody against the human transferrin receptor. The cells with positive reaction were recognized in the basal and parabasal layers of the normal epithelium. The staining was found in all the malignant tumors but not in the benign tumors. These results suggest that the immunohistochemical analysis of the transferrin receptor is useful for the diagnosis of oral malignant tumor in addition to the clinical and pathological examinations.

用人转铁蛋白受体单克隆抗体染色检测口腔肿瘤组织中转铁蛋白受体的表达。阳性反应的细胞分布在正常上皮的基底层和准基底层。恶性肿瘤均可见染色,良性肿瘤未见染色。提示转铁蛋白受体的免疫组化分析除临床和病理检查外,对口腔恶性肿瘤的诊断有重要意义。
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引用次数: 0
Protective effects of calcium antagonist (nitrendipine) on calcium ionophore A23187-induced liver cell injury. 钙拮抗剂尼群地平对钙离子载体a23187诱导的肝细胞损伤的保护作用。
S Matsuda

Calcium ionophore A23187-induced liver damage was studied in perfused rat liver and isolated hepatocytes. Two groups of calcium antagonists, dihydropyridine-type and nondihydropyridine-type, were examined for their protective effects on A23187-induced liver damage. The former calcium antagonist inhibited cell damage at pharmacological doses, whereas nondihydropyridine-type calcium antagonists were unable to prevent A23187-induced cytotoxicity. Different inhibitors, such as cAMP phosphodiesterase inhibitor OPC3689, calmodulin inhibitor W-7, and C-kinase inhibitor H-7, were tested in isolated hepatocytes to determine whether intracellular signal transduction systems are involved in the liver cell injury produced by A23187. Calcium ionophore A23187-induced LDH activity of the medium was depressed by W-7 and H-7 to 55 +/- 4% and 63 +/- 4% of controls (p less than 0.01), respectively. However, OPC3689 did not show a protective effect. We conclude that A23187-induced liver cell injury was inhibited by dihydropyridine-type calcium antagonists which may interfere with activation of calmodulin and C-kinase.

研究了钙离子载体a23187对灌注大鼠肝脏和离体肝细胞的损伤作用。研究了两组钙拮抗剂(二氢吡啶型和非二氢吡啶型)对a23187肝损伤的保护作用。前一种钙拮抗剂在药理学剂量下抑制细胞损伤,而非二氢吡啶型钙拮抗剂无法阻止a23187诱导的细胞毒性。不同的抑制剂,如cAMP磷酸二酯酶抑制剂OPC3689,钙调素抑制剂W-7和c激酶抑制剂H-7,在分离的肝细胞中进行了测试,以确定细胞内信号转导系统是否参与了A23187引起的肝细胞损伤。W-7和H-7对钙离子载体a23187诱导的LDH活性的抑制作用分别为对照的55 +/- 4%和63 +/- 4% (p < 0.01)。然而,OPC3689没有显示出保护作用。我们认为,二氢吡啶型钙拮抗剂可以抑制a23187诱导的肝细胞损伤,这种拮抗剂可能干扰钙调素和c激酶的激活。
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引用次数: 0
Enzyme-linked immunosorbent assay of SIgA in whole saliva of healthy subjects and patients with oral diseases. 健康人与口腔疾病患者全唾液SIgA的酶联免疫吸附测定。
K Sato

The author tried to determine the secretory immunoglobulin A (SIgA) level in the whole saliva of healthy subjects and patients with oral diseases by the enzyme-linked immunosorbent assay (ELISA) technique. An ELISA system that was previously developed for determining the SIgA level in the pancreatic juice was modified and was proved to be applicable for the SIgA in the whole saliva. It was found that the SIgA in the whole saliva could be determined with excellent reproducibility and linearity when the samples were diluted 1,000-fold. This assay method was superior to the conventional techniques in terms of accuracy, covering a wide range SIgA levels in the saliva. In the 74 healthy subjects, the SIgA level in the whole saliva did not show any major age- or sex-related difference. However, patients with leukoplakia, lichen planus and carcinoma in the oral cavity showed higher levels of SIgA. The elevated levels of SIgA in the saliva of these patients suggested that some local changes in immunological competence might occur due to prolonged exposure to the antigen in the oral cavity.

采用酶联免疫吸附试验(ELISA)技术测定健康人及口腔疾病患者全唾液中分泌性免疫球蛋白A (SIgA)水平。改进了先前开发的用于测定胰液中SIgA水平的ELISA系统,并证明该系统适用于整个唾液中的SIgA。结果表明,当样品被稀释1000倍时,全唾液中SIgA的测定具有良好的重现性和线性。该分析方法在准确性方面优于传统技术,覆盖了唾液中广泛的SIgA水平。在74名健康受试者中,整个唾液中的SIgA水平没有显示出任何主要的年龄或性别差异。然而,口腔白斑、扁平苔藓和癌患者的SIgA水平较高。这些患者唾液中SIgA水平的升高表明,由于长期暴露于口腔内的抗原,可能会发生局部免疫能力的变化。
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引用次数: 0
Three-dimensional digitization: method for evaluating morphological differences between teeth. 三维数字化:评估牙齿形态差异的方法。
K Soma, T Kuroki, T Nakamura, F Miura

A new comparative method has been developed for evaluating morphological differences between a pair of teeth. This method consists of five steps: 1) digitization of the surface morphology of the teeth, 2) standardization to enable comparison of the teeth on a common axis, 3) reversal of data on one tooth to enable comparison with the opposite tooth, 4) qualitative evaluation by computer graphic superimposition and 5) quantitative evaluation by the set operation. To illustrate its effectiveness, this method was applied to a pair of extracted premolars. Application to the dental crown on a clinical plaster cast was also discussed.

一种新的比较方法已经开发评估形态差异的一对牙齿。该方法包括五个步骤:1)牙齿表面形态的数字化;2)标准化以实现牙齿在共同轴上的比较;3)对一个牙齿的数据进行反转以实现与相反牙齿的比较;4)通过计算机图形叠加进行定性评价;5)通过集合运算进行定量评价。为了说明该方法的有效性,将该方法应用于一对拔出的前磨牙。并讨论了在牙冠石膏石膏上的应用。
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引用次数: 0
Three-dimensional digitization: method for evaluating morphological differences between teeth. 三维数字化:评估牙齿形态差异的方法。
Pub Date : 1991-03-01 DOI: 10.11480/BTMD.380101
K. Soma, T. Kuroki, T. Nakamura, F. Miura
A new comparative method has been developed for evaluating morphological differences between a pair of teeth. This method consists of five steps: 1) digitization of the surface morphology of the teeth, 2) standardization to enable comparison of the teeth on a common axis, 3) reversal of data on one tooth to enable comparison with the opposite tooth, 4) qualitative evaluation by computer graphic superimposition and 5) quantitative evaluation by the set operation. To illustrate its effectiveness, this method was applied to a pair of extracted premolars. Application to the dental crown on a clinical plaster cast was also discussed.
一种新的比较方法已经开发评估形态差异的一对牙齿。该方法包括五个步骤:1)牙齿表面形态的数字化;2)标准化以实现牙齿在共同轴上的比较;3)对一个牙齿的数据进行反转以实现与相反牙齿的比较;4)通过计算机图形叠加进行定性评价;5)通过集合运算进行定量评价。为了说明该方法的有效性,将该方法应用于一对拔出的前磨牙。并讨论了在牙冠石膏石膏上的应用。
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引用次数: 0
期刊
The Bulletin of Tokyo Medical and Dental University
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