Pub Date : 2022-08-15DOI: 10.31254/phyto.2022.11415
Rathod Db, Rajput Hj, Katore Td, Shinde Py
A laboratory experiment was conducted on French beans (phaseolus vulgaris L.) to investigate the effect of organic and inorganic seed treatments on seed health. The seeds of French bean were subjected to different seed treatments viz., T0: Control (untreated), T1: Neem leaf powder @5g/kg of seed, T2: neem oil @5ml/kg of seed, T3: Castor oil @5ml/kg of seed, T4: karanj oil @5ml/kg of seed, T5: vekhand powder @10g/kg of seed, T6: Termuric powder @5g/kg of seed, T7: Citronella oil @5ml/kg of seed, T8: Ash @5g/kg of seeds, T9: Deltamethrin @0.04ml/kg of seed. Seed health was tested for different parameters viz., pulse beetle infestation, seed weight loss, egg-laying, adult emergence, percent mortality, dead insects and live insects. The bio-efficacy test was undertaken to find out the effect of chemicals and different botanicals against pulse beetle in French bean seed. Results revealed that seeds treated with Deltamethrin @0.04ml/kg of seed significantly recorded the lowest pulse beetle infestation (1.33%), lower number of egg-laying (11.00), lowest number of adult emergence (1.67), lowest seed weight loss (4.67%), highest mortality (87.8%), higher dead insect (15.00) and lower live insect (5.00) at the end of storage period. Among the botanicals, seed treated with neem oil @5 ml/kg of seed showed higher seed quality and health parameters. From the present investigation, the seed treated with organic and inorganic components protected the seed health while, the control seed deteriorated at the end of the storage period.
{"title":"Effect of organic and inorganic seed treatments on seed health of French bean (Phaseolus vulgaris L.)","authors":"Rathod Db, Rajput Hj, Katore Td, Shinde Py","doi":"10.31254/phyto.2022.11415","DOIUrl":"https://doi.org/10.31254/phyto.2022.11415","url":null,"abstract":"A laboratory experiment was conducted on French beans (phaseolus vulgaris L.) to investigate the effect of organic and inorganic seed treatments on seed health. The seeds of French bean were subjected to different seed treatments viz., T0: Control (untreated), T1: Neem leaf powder @5g/kg of seed, T2: neem oil @5ml/kg of seed, T3: Castor oil @5ml/kg of seed, T4: karanj oil @5ml/kg of seed, T5: vekhand powder @10g/kg of seed, T6: Termuric powder @5g/kg of seed, T7: Citronella oil @5ml/kg of seed, T8: Ash @5g/kg of seeds, T9: Deltamethrin @0.04ml/kg of seed. Seed health was tested for different parameters viz., pulse beetle infestation, seed weight loss, egg-laying, adult emergence, percent mortality, dead insects and live insects. The bio-efficacy test was undertaken to find out the effect of chemicals and different botanicals against pulse beetle in French bean seed. Results revealed that seeds treated with Deltamethrin @0.04ml/kg of seed significantly recorded the lowest pulse beetle infestation (1.33%), lower number of egg-laying (11.00), lowest number of adult emergence (1.67), lowest seed weight loss (4.67%), highest mortality (87.8%), higher dead insect (15.00) and lower live insect (5.00) at the end of storage period. Among the botanicals, seed treated with neem oil @5 ml/kg of seed showed higher seed quality and health parameters. From the present investigation, the seed treated with organic and inorganic components protected the seed health while, the control seed deteriorated at the end of the storage period.","PeriodicalId":22851,"journal":{"name":"The Journal of Phytopharmacology","volume":"22 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75714987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-08-15DOI: 10.31254/phyto.2022.11409
O. Ngassapa, Deborah Kiumlo Balira Runyoro, S. Maregesi, Raphael Zozimus Sangeda, Stephen Msemo, Rogers Mwakalukwa, Abdul W. Kidukuli, E. Marandu
Background: Propolis has been traditionally used in many countries for management of various health conditions and many previous studies have shown that it has many biological activities, including antimicrobial activity and cytotoxicity. However, not much has been studied with regard to Tanzanian propolis; hence this study reports biological activity of propolis collected from 8 regions of Tanzania. Objectives: The purpose of this study was to evaluate the antimicrobial activity and potential cytotoxicity of propolis samples collected from various regions of Tanzania. Metet hods: Ethanolic extracts of 28 propolis samples collected from 8 regions in Tanzania were evaluated for antimicrobial activity against Gram +ve bacteria, Gram -ve bacteria and fungi, using the broth microdilution method, through which minimum inhibitory concentrations were determined. Brine shrimp lethality test was done using Artemia salina nauplii, as a preliminary indication of potential anticancer activity. Results: Out of 28 tested propolis samples, 21 exhibited antimicrobial activity on one or more microorganisms at the tested concentrations, with MICs of 1.25 – 5.0 mg/ml. Fourteen out of 28 samples were found to be toxic on brine shrimps, with a sample from Singida region being the most toxic. Conclusion: Propolis samples from various regions of Tanzania demonstrated antimicrobial activity on some microorganisms, indicating that they are a potential source of antimicrobial agents. Some samples also exhibited brine shrimp toxicity; implying that they may also be toxic on cancer cells and thus, may be a potential source of anticancer agents. They may also be a source of compounds with other biological activities.
{"title":"Antimicrobial activity and brine shrimp toxicity of propolis collected from various regions of Tanzania","authors":"O. Ngassapa, Deborah Kiumlo Balira Runyoro, S. Maregesi, Raphael Zozimus Sangeda, Stephen Msemo, Rogers Mwakalukwa, Abdul W. Kidukuli, E. Marandu","doi":"10.31254/phyto.2022.11409","DOIUrl":"https://doi.org/10.31254/phyto.2022.11409","url":null,"abstract":"Background: Propolis has been traditionally used in many countries for management of various health conditions and many previous studies have shown that it has many biological activities, including antimicrobial activity and cytotoxicity. However, not much has been studied with regard to Tanzanian propolis; hence this study reports biological activity of propolis collected from 8 regions of Tanzania. Objectives: The purpose of this study was to evaluate the antimicrobial activity and potential cytotoxicity of propolis samples collected from various regions of Tanzania. Metet hods: Ethanolic extracts of 28 propolis samples collected from 8 regions in Tanzania were evaluated for antimicrobial activity against Gram +ve bacteria, Gram -ve bacteria and fungi, using the broth microdilution method, through which minimum inhibitory concentrations were determined. Brine shrimp lethality test was done using Artemia salina nauplii, as a preliminary indication of potential anticancer activity. Results: Out of 28 tested propolis samples, 21 exhibited antimicrobial activity on one or more microorganisms at the tested concentrations, with MICs of 1.25 – 5.0 mg/ml. Fourteen out of 28 samples were found to be toxic on brine shrimps, with a sample from Singida region being the most toxic. Conclusion: Propolis samples from various regions of Tanzania demonstrated antimicrobial activity on some microorganisms, indicating that they are a potential source of antimicrobial agents. Some samples also exhibited brine shrimp toxicity; implying that they may also be toxic on cancer cells and thus, may be a potential source of anticancer agents. They may also be a source of compounds with other biological activities.","PeriodicalId":22851,"journal":{"name":"The Journal of Phytopharmacology","volume":"93 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81746577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-08-15DOI: 10.31254/phyto.2022.11413
H. Saxena, Asima Das, S. Parihar
Dillenia pentagyna often known as ‘Karmal’ is a member of Dilleniaceae. Different parts of the plant have been used by tribal communities to treat a broad spectrum of ailments. The plant reports the presence of some vital therapeutic compounds such as botulin, botulinic acid, botulinic acid, morolic acid, lupeol, diploic acid, etc. Some of the major pharmaceutical properties of the plant are anticancerous, anti-fungal, anti-bacterial, and anti-diabetic. This plant has been recently classified as a critically endangered species in Madhya Pradesh state. Low germination rates are one of the major factors of being a critically endangered species. Despite being a potent curative plant species, it is yet to be fully explored. The objective of the present review to highlight the phytochemical investigations and pharmacology applications for several purposes like anti-diabetic, anti-cancerous, anti-microbial, antioxidant, etc of this plant species. In future studies, this knowledge can be applied to the safe and evidence-based use of traditional Indian medicinal plants in global Phyto pharmacotherapy and to the discovery of novel leads for the development of herbal formulations.
{"title":"Dillenia pentagyna Roxb.: A Review on Phytochemistry and Pharmacology","authors":"H. Saxena, Asima Das, S. Parihar","doi":"10.31254/phyto.2022.11413","DOIUrl":"https://doi.org/10.31254/phyto.2022.11413","url":null,"abstract":"Dillenia pentagyna often known as ‘Karmal’ is a member of Dilleniaceae. Different parts of the plant have been used by tribal communities to treat a broad spectrum of ailments. The plant reports the presence of some vital therapeutic compounds such as botulin, botulinic acid, botulinic acid, morolic acid, lupeol, diploic acid, etc. Some of the major pharmaceutical properties of the plant are anticancerous, anti-fungal, anti-bacterial, and anti-diabetic. This plant has been recently classified as a critically endangered species in Madhya Pradesh state. Low germination rates are one of the major factors of being a critically endangered species. Despite being a potent curative plant species, it is yet to be fully explored. The objective of the present review to highlight the phytochemical investigations and pharmacology applications for several purposes like anti-diabetic, anti-cancerous, anti-microbial, antioxidant, etc of this plant species. In future studies, this knowledge can be applied to the safe and evidence-based use of traditional Indian medicinal plants in global Phyto pharmacotherapy and to the discovery of novel leads for the development of herbal formulations.","PeriodicalId":22851,"journal":{"name":"The Journal of Phytopharmacology","volume":"71 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85183590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-08-15DOI: 10.31254/phyto.2022.11410
Adheena Xavier, S. Padma Rani, R. Shankar, A. Nisha, S. Sujith, R. Uma
Essential oils, which are the plant derived secondary metabolites have been reported for various traditional medicinal applications. Amongst them, lemongrass oil (LGO) derived from Cymbopogon spp. as well as its major constituent citral possess a myriad of therapeutic potentials. The present study has been undertaken to study the adverse effects of LGO and citral on acute oral exposure to Sprague Dawley rats to establish the preliminary safety of these compounds prior to their efficacy evaluation against fatty liver disease. The toxicity study was conducted as per OECD guidelines No. 420. The LGO and citral were solubilized in 1% tween 80 and administered orally in a sequential manner in one animal at 2000 mg/kg (sighting study) followed by four animals (main study). The animals were then monitored for any clinical abnormalities or mortality and body weight gain during the observational period of 14- days, after which the animals were sacrificed and examined for abnormal lesions. LGO was further subjected to gas chromatography-mass spectrometry (GC-MS) analysis to characterize its chemical constituents, which revealed alpha and beta citral as the two major constituents. The rats treated with LGO and citral survived throughout the study period and didn’t exhibit any clinical abnormalities. Moreover, body weight gain was comparable to the vehicle treated rats and necropsy revealed no pathological alterations. Thus, the present study indicated LGO and citral as safe compounds with an LD50 greater than 2000 mg/kg and could be labelled as category 5/unclassified in hazard category of Globally harmonized system for classification of chemicals.
{"title":"Evaluation of acute oral toxicity of lemon grass oil and citral in albino rats","authors":"Adheena Xavier, S. Padma Rani, R. Shankar, A. Nisha, S. Sujith, R. Uma","doi":"10.31254/phyto.2022.11410","DOIUrl":"https://doi.org/10.31254/phyto.2022.11410","url":null,"abstract":"Essential oils, which are the plant derived secondary metabolites have been reported for various traditional medicinal applications. Amongst them, lemongrass oil (LGO) derived from Cymbopogon spp. as well as its major constituent citral possess a myriad of therapeutic potentials. The present study has been undertaken to study the adverse effects of LGO and citral on acute oral exposure to Sprague Dawley rats to establish the preliminary safety of these compounds prior to their efficacy evaluation against fatty liver disease. The toxicity study was conducted as per OECD guidelines No. 420. The LGO and citral were solubilized in 1% tween 80 and administered orally in a sequential manner in one animal at 2000 mg/kg (sighting study) followed by four animals (main study). The animals were then monitored for any clinical abnormalities or mortality and body weight gain during the observational period of 14- days, after which the animals were sacrificed and examined for abnormal lesions. LGO was further subjected to gas chromatography-mass spectrometry (GC-MS) analysis to characterize its chemical constituents, which revealed alpha and beta citral as the two major constituents. The rats treated with LGO and citral survived throughout the study period and didn’t exhibit any clinical abnormalities. Moreover, body weight gain was comparable to the vehicle treated rats and necropsy revealed no pathological alterations. Thus, the present study indicated LGO and citral as safe compounds with an LD50 greater than 2000 mg/kg and could be labelled as category 5/unclassified in hazard category of Globally harmonized system for classification of chemicals.","PeriodicalId":22851,"journal":{"name":"The Journal of Phytopharmacology","volume":"37 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78357776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-08-15DOI: 10.31254/phyto.2022.11406
E. Agbaje, Omiyale Olumakinde Charles
Health problems relating to wound healing remain a significant issue in health management. Various formulations of Spondias mombin Linn. (Anacardiaceous) serves potent roles in ethnomedicine. To explore the counter-inflammatory and cytokines modulatory roles together with the antioxidant and wound healing properties of Spondias mombin Linn. (Anacardiaceous) leaves essential oil (SMEO) on excised wounds. Spondias mombin Linn. (Anacardiaceous) leaves essential oil was isolated through hydro distillation essential in Clevenger type apparatus (Borosil, India). Then thirty-eight healthy adult male albino rats (250 ± 20g) were grouped randomly into (n=6); Group 1- 50 µL 1% SMEO, Group 2- 0.1% DMSO and Tween 20 (Control), Group 3- Dermazin® ointment, Group 4-untreated, Group 5-50µL 10% SMEO, Group 6- 50µL 15% SMEO, with two unwounded samples, were treated for 14 days. Tissues of two rats was harvested per group on the 3 rd, 10th and 14th days after excision, wounded areas were excised for RT-qPCR cytokines analysis and gene expression (Light Cycler, Mannheim, Germany) (IL 6, IL1β, TNFα). The SMEO of (25–100 μg/ml) was passed through Total antioxidant/flavonoid/phenol phytochemical estimation assays. The assays showed high presence of flavonoids/phenols. The 10% and 15% essential oil healing progression showed efficiency over the standard. Cytokine analysis/gene expression revealed a significant reduction in IL6 levels, a key regulator of other pro-inflammatory cytokines and reparative process, after the 3rd, 7th and 14th day. The extracted Spondias mombin Linn. essential oil showed stimulation of an anti-inflammatory effect through a significant reduction of IL-6 levels as compared to the vehicle and standard treatment on the 3 rd, 10th through to the 14th day of the treatment course, neo-vascularization, tissue regeneration phase and overall improved wound healing better than the standard.
{"title":"Anti-inflammatory and Cytokines Modulatory Activities of Spondias mombin Linn. (Anacardiacea) in Wound Healing: Roles of IL6","authors":"E. Agbaje, Omiyale Olumakinde Charles","doi":"10.31254/phyto.2022.11406","DOIUrl":"https://doi.org/10.31254/phyto.2022.11406","url":null,"abstract":"Health problems relating to wound healing remain a significant issue in health management. Various formulations of Spondias mombin Linn. (Anacardiaceous) serves potent roles in ethnomedicine. To explore the counter-inflammatory and cytokines modulatory roles together with the antioxidant and wound healing properties of Spondias mombin Linn. (Anacardiaceous) leaves essential oil (SMEO) on excised wounds. Spondias mombin Linn. (Anacardiaceous) leaves essential oil was isolated through hydro distillation essential in Clevenger type apparatus (Borosil, India). Then thirty-eight healthy adult male albino rats (250 ± 20g) were grouped randomly into (n=6); Group 1- 50 µL 1% SMEO, Group 2- 0.1% DMSO and Tween 20 (Control), Group 3- Dermazin® ointment, Group 4-untreated, Group 5-50µL 10% SMEO, Group 6- 50µL 15% SMEO, with two unwounded samples, were treated for 14 days. Tissues of two rats was harvested per group on the 3 rd, 10th and 14th days after excision, wounded areas were excised for RT-qPCR cytokines analysis and gene expression (Light Cycler, Mannheim, Germany) (IL 6, IL1β, TNFα). The SMEO of (25–100 μg/ml) was passed through Total antioxidant/flavonoid/phenol phytochemical estimation assays. The assays showed high presence of flavonoids/phenols. The 10% and 15% essential oil healing progression showed efficiency over the standard. Cytokine analysis/gene expression revealed a significant reduction in IL6 levels, a key regulator of other pro-inflammatory cytokines and reparative process, after the 3rd, 7th and 14th day. The extracted Spondias mombin Linn. essential oil showed stimulation of an anti-inflammatory effect through a significant reduction of IL-6 levels as compared to the vehicle and standard treatment on the 3 rd, 10th through to the 14th day of the treatment course, neo-vascularization, tissue regeneration phase and overall improved wound healing better than the standard.","PeriodicalId":22851,"journal":{"name":"The Journal of Phytopharmacology","volume":"50 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87126416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-08-15DOI: 10.31254/phyto.2022.11405
S. Ray, A. Taraphdar, M. Gupta
Since ancient times, Amalakyadi Gana, a polyherbal formulation of Susruta Samhita (6th century BCE), has been used for the prevention and treatment of numerous gastrointestinal diseases. This formulation consists of fruits of Phyllanthus emblica, Terminalia chebula, Piper longum, and the root of Plumbago zeylanica. The hepatoprotective efficacy of this formulation was evaluated following the acute toxicity study in mice to validate its ayurvedic uses. The hepatoprotective efficacy was assessed using paracetamol-induced hepatotoxicity in Swiss albino mice. Research drug exhibited in normalizing the PCM-dependent rise of serum liver function markers. After administration of the aqueous extract of Amalakyadi Gana, relevant blood biochemical measures showed significant (P < 0.05) hepatoprotective activity in a dosage-dependent manner, especially at the dose of 700 mg/kg orally in mice. When compared to the control group, significant (p < 0.05) histological alterations were also observed in the liver tissues. This formulation exhibited results in normalizing the liver architecture by decreasing necrotic foci along with the normal liver parenchymal structure in the research drug pre-treated groups mainly at the dose of 700 mg/kg, caused due to paracetamol toxicity. The research drug's sustained activity was comparable to that of the silymarin (200 mg/kg, p.o.) reference medicine. This formulation possesses significant hepatoprotective activity without any toxicity in mice.
{"title":"Hepatoprotective activity of Amalakyadi Gana, a polyherbal ayurvedic formulation in paracetamol-induced hepatotoxicity in mice","authors":"S. Ray, A. Taraphdar, M. Gupta","doi":"10.31254/phyto.2022.11405","DOIUrl":"https://doi.org/10.31254/phyto.2022.11405","url":null,"abstract":"Since ancient times, Amalakyadi Gana, a polyherbal formulation of Susruta Samhita (6th century BCE), has been used for the prevention and treatment of numerous gastrointestinal diseases. This formulation consists of fruits of Phyllanthus emblica, Terminalia chebula, Piper longum, and the root of Plumbago zeylanica. The hepatoprotective efficacy of this formulation was evaluated following the acute toxicity study in mice to validate its ayurvedic uses. The hepatoprotective efficacy was assessed using paracetamol-induced hepatotoxicity in Swiss albino mice. Research drug exhibited in normalizing the PCM-dependent rise of serum liver function markers. After administration of the aqueous extract of Amalakyadi Gana, relevant blood biochemical measures showed significant (P < 0.05) hepatoprotective activity in a dosage-dependent manner, especially at the dose of 700 mg/kg orally in mice. When compared to the control group, significant (p < 0.05) histological alterations were also observed in the liver tissues. This formulation exhibited results in normalizing the liver architecture by decreasing necrotic foci along with the normal liver parenchymal structure in the research drug pre-treated groups mainly at the dose of 700 mg/kg, caused due to paracetamol toxicity. The research drug's sustained activity was comparable to that of the silymarin (200 mg/kg, p.o.) reference medicine. This formulation possesses significant hepatoprotective activity without any toxicity in mice.","PeriodicalId":22851,"journal":{"name":"The Journal of Phytopharmacology","volume":"46 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86223235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-08-15DOI: 10.31254/phyto.2022.11407
P. Chhalliyil, Usha Nandini, Neelima Thota
In this current case report, A 54-year-old non-alcoholic, married woman presented with chief complaints of abdominal pain and unbearable pain while passing urine, CT scan revealed an enlarged liver (16.2 cm in size) and a thick-walled cystic lesion measuring (4.3*4.1*6.0 in the right ovary adnexa) on 17th October 2020. The patient started following Uni5 treatment in November 2020 and continued the treatment until she was cured completely. Based on the five elements principle, she was treated for four months with Uni5 cleansing treatment, hot oil fomentation, liver tonic-rich herbs, and probiotics-rich food. Her condition was monitored through a CT scan, and after two months she was relieved from uterine fibroid and liver enlargement. This indicates that there is an association between Gut microbiomes, fatty liver, and polycystic ovarian syndrome. Therefore, a uterine fibroid can be entirely cured through Uni5 treatment.
{"title":"Uni5 Approach to Treat PCOS associated with Non-alcoholic Fatty Liver Disease: A Case Report","authors":"P. Chhalliyil, Usha Nandini, Neelima Thota","doi":"10.31254/phyto.2022.11407","DOIUrl":"https://doi.org/10.31254/phyto.2022.11407","url":null,"abstract":"In this current case report, A 54-year-old non-alcoholic, married woman presented with chief complaints of abdominal pain and unbearable pain while passing urine, CT scan revealed an enlarged liver (16.2 cm in size) and a thick-walled cystic lesion measuring (4.3*4.1*6.0 in the right ovary adnexa) on 17th October 2020. The patient started following Uni5 treatment in November 2020 and continued the treatment until she was cured completely. Based on the five elements principle, she was treated for four months with Uni5 cleansing treatment, hot oil fomentation, liver tonic-rich herbs, and probiotics-rich food. Her condition was monitored through a CT scan, and after two months she was relieved from uterine fibroid and liver enlargement. This indicates that there is an association between Gut microbiomes, fatty liver, and polycystic ovarian syndrome. Therefore, a uterine fibroid can be entirely cured through Uni5 treatment.","PeriodicalId":22851,"journal":{"name":"The Journal of Phytopharmacology","volume":"106 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77054700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-08-15DOI: 10.31254/phyto.2022.11414
Yu Peng, Y. Gong, Congwei Wang, Dujuan Shi, Jinyan Zhang, G. W. K. Zhang, Xinzhou Yang, Xiaojun Li
(+)-(R)-de-O-methyllasiodiplodin (DOML), isolated from the Chinese herbal medicine Ludwigia hyssop folia, has great potential for development in pharmacological research on hepatocellular carcinoma (HCC). In our study, the CCK-8 assay, morphological observation, flow cytometry (also known as Annexin V-FITC/PI double staining), as well as Western blotting were adopted to study the anti-liver cancer activity and mechanisms of DOML on HepG2 and HuH-7 cells. The research exhibited that DOML dose- and time-dependently reduced the cell viability of HCC cells. DOML treatment resulted in changes in cell morphology, such as irregular edges, reduced volume, and decreased adhesion were observed under the microscope. Flow cytometry analysis indicated that apoptosis is the major form of cell death. In addition, blocking autophagy and necroptosis pathways couldn’t alleviate DOML-induced apoptosis. Protein expression levels of Bax, activated Caspase-3 and Caspase-9, and PARP were increased, while Bcl-2 protein levels were reduced by DOML treatment, which suggested that the mitochondrial apoptotic pathway may be involved in DOML-induced cell death. Moreover, the expression of NF-κB and the phosphorylation of Akt and STAT3 decreased with the increase of dosage, suggesting that the apoptotic mechanism might be related to the Akt/NF-κB and STAT3 signaling pathways. All these results indicate that DOML has the potential effects of anti-hepatoma.
(+)-(R)-de- o -methyllasiodiplodin (DOML)从中草药Ludwigia hyssop folia中分离得到，在肝细胞癌(HCC)的药理研究中具有很大的开发潜力。本研究采用CCK-8实验、形态学观察、流式细胞术(又称Annexin V-FITC/PI双染色)、Western blotting等方法研究了DOML对HepG2和HuH-7细胞的抗肝癌活性及其作用机制。研究表明，DOML降低HCC细胞的细胞活力具有剂量和时间依赖性。DOML处理导致细胞形态学改变，显微镜下观察到细胞边缘不规则，体积减小，黏附降低。流式细胞术分析表明，细胞凋亡是细胞死亡的主要形式。此外，阻断自噬和坏死下垂途径不能减轻doml诱导的细胞凋亡。经DOML处理后，Bax、活化Caspase-3、Caspase-9和PARP蛋白表达水平升高，Bcl-2蛋白表达水平降低，提示线粒体凋亡途径可能参与了DOML诱导的细胞死亡。NF-κB表达和Akt、STAT3磷酸化水平均随剂量增加而降低，提示凋亡机制可能与Akt/NF-κB和STAT3信号通路有关。这些结果表明，DOML具有潜在的抗肝癌作用。
{"title":"de-O-methyllasiodiplodin from Ludwigia hyssopifolia causes death of human liver cancer cells through the mitochondrial apoptotic, Akt/NF-κB and STAT3 pathway in vitro","authors":"Yu Peng, Y. Gong, Congwei Wang, Dujuan Shi, Jinyan Zhang, G. W. K. Zhang, Xinzhou Yang, Xiaojun Li","doi":"10.31254/phyto.2022.11414","DOIUrl":"https://doi.org/10.31254/phyto.2022.11414","url":null,"abstract":"(+)-(R)-de-O-methyllasiodiplodin (DOML), isolated from the Chinese herbal medicine Ludwigia hyssop folia, has great potential for development in pharmacological research on hepatocellular carcinoma (HCC). In our study, the CCK-8 assay, morphological observation, flow cytometry (also known as Annexin V-FITC/PI double staining), as well as Western blotting were adopted to study the anti-liver cancer activity and mechanisms of DOML on HepG2 and HuH-7 cells. The research exhibited that DOML dose- and time-dependently reduced the cell viability of HCC cells. DOML treatment resulted in changes in cell morphology, such as irregular edges, reduced volume, and decreased adhesion were observed under the microscope. Flow cytometry analysis indicated that apoptosis is the major form of cell death. In addition, blocking autophagy and necroptosis pathways couldn’t alleviate DOML-induced apoptosis. Protein expression levels of Bax, activated Caspase-3 and Caspase-9, and PARP were increased, while Bcl-2 protein levels were reduced by DOML treatment, which suggested that the mitochondrial apoptotic pathway may be involved in DOML-induced cell death. Moreover, the expression of NF-κB and the phosphorylation of Akt and STAT3 decreased with the increase of dosage, suggesting that the apoptotic mechanism might be related to the Akt/NF-κB and STAT3 signaling pathways. All these results indicate that DOML has the potential effects of anti-hepatoma.","PeriodicalId":22851,"journal":{"name":"The Journal of Phytopharmacology","volume":"107 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81080546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-08-15DOI: 10.31254/phyto.2022.11402
Wambugu N. Enoc, M. Maina, N. Eliud
Several sex selection methods are available but with varying success rates, availability, and affordability. Vernonia amygdalina has been used by some Kenyan communities for the male child pre-selection at conception but these claims have not been scientifically ascertained. This study aims at evaluating the ability of this herbal plant to pre-select for the male child at conception. The roots of the plant were collected and processed before extraction using methanol. The obtained crude plant extracts were orally administered to Rattus norvegicus rats before and during mating for one week. Crude extracts at doses of 50, 87, and 150mg/kg body weight were used to evaluate the effects of the plant on the sex ratio of pups and factors related to sex ratio. A P value of P<0.05 was set as the limit of significance. The extracts were also subjected to quantitative phytochemical and mineral analysis. The results indicated that treating female rats with the plant extract significantly increased the ratio of male to female pups in all the treatment groups. There was a significant decrease in serum cortisol levels in both male and female rats and an increase in vaginal pH in female rats. The 50 and 87 mg/kg doses caused a significant increase in estrogen levels compared to the untreated group. These results validated the use of the plant by herbalists for the preselection of a male child at conception.
{"title":"Methanol extracts of Vernonia amygdalina Del increase the sex ratio of offspring in Rattus norvegicus rats","authors":"Wambugu N. Enoc, M. Maina, N. Eliud","doi":"10.31254/phyto.2022.11402","DOIUrl":"https://doi.org/10.31254/phyto.2022.11402","url":null,"abstract":"Several sex selection methods are available but with varying success rates, availability, and affordability. Vernonia amygdalina has been used by some Kenyan communities for the male child pre-selection at conception but these claims have not been scientifically ascertained. This study aims at evaluating the ability of this herbal plant to pre-select for the male child at conception. The roots of the plant were collected and processed before extraction using methanol. The obtained crude plant extracts were orally administered to Rattus norvegicus rats before and during mating for one week. Crude extracts at doses of 50, 87, and 150mg/kg body weight were used to evaluate the effects of the plant on the sex ratio of pups and factors related to sex ratio. A P value of P<0.05 was set as the limit of significance. The extracts were also subjected to quantitative phytochemical and mineral analysis. The results indicated that treating female rats with the plant extract significantly increased the ratio of male to female pups in all the treatment groups. There was a significant decrease in serum cortisol levels in both male and female rats and an increase in vaginal pH in female rats. The 50 and 87 mg/kg doses caused a significant increase in estrogen levels compared to the untreated group. These results validated the use of the plant by herbalists for the preselection of a male child at conception.","PeriodicalId":22851,"journal":{"name":"The Journal of Phytopharmacology","volume":"138 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77431177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-25DOI: 10.31254/phyto.2022.11303
V. Nyigo, H. Malebo, F. Mabiki, R. Mdegela
Purification of dichloromethane extract from root barks and leaves of Synadenium glaucescens extracts through chromatographic techniques resulted into the isolation of two compounds, namely erythrinacinate C and 1-octacosanol. Chemical structures were established mainly using both one and two dimensional 1H and 13C NMR data and by comparison of the current NMR data with those from literature. Mass spectrophotometry data were used for confirmation through molecular ion peak. Both compounds are known to have been isolated from other plant species but are being reported from this plant species for the first time.
{"title":"Isolation and identification of long -chain aliphatic compounds from Synadenium glaucescens","authors":"V. Nyigo, H. Malebo, F. Mabiki, R. Mdegela","doi":"10.31254/phyto.2022.11303","DOIUrl":"https://doi.org/10.31254/phyto.2022.11303","url":null,"abstract":"Purification of dichloromethane extract from root barks and leaves of Synadenium glaucescens extracts through chromatographic techniques resulted into the isolation of two compounds, namely erythrinacinate C and 1-octacosanol. Chemical structures were established mainly using both one and two dimensional 1H and 13C NMR data and by comparison of the current NMR data with those from literature. Mass spectrophotometry data were used for confirmation through molecular ion peak. Both compounds are known to have been isolated from other plant species but are being reported from this plant species for the first time.","PeriodicalId":22851,"journal":{"name":"The Journal of Phytopharmacology","volume":"3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88308953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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