Transforming growth factor betas are multifunctional polypeptides in the cytokine superfamily. They have a growth inhibitory role on hemopoietic pro genitor cells in semisolid colony assay as well as in long-term bone-marrow cul ture. TGF -�2 represses stromal cells, stem cell factor gene transcription, and de creases the stability of c-kit transcripts in hemopoietic cells. TGF-� also modu lates GM-CSF production from human lymphocytes. The present study reveals the TGF-�2 role in production of GM-CSF in HTB 5637, human bladder carci noma cell line. HTB 5637 cells were treated with 5 ng/mL of human TGF -�?' viable cells were counted and GM-CSF concentration was determined, No anti proliferate activity of TGF-�2 on HTB 5637 cell line was observed. Biological assay showed increased levels of GM-CSF in the supernatant of cultured cells. However this increase was lower than that expected from ELISA. Since TGF-� may be an active suppressor factor regulating hemopoiesis, it seems that some inhibitory factor(s) may be produced (increased) in response to TGF-�2 treat ment. It has been shown that GM-CSF mRNA content from HTB 5637 cell line is very stable and this stabilization is translational dependent. Using Slot blot and Northern blot analysis, we determined that TGF-�2 upregulated GM-CSF gene expression in HTB 5637 cell line. The results suggest that TGF-�2 upregulates the production of GM-CSF gene at the transcriptional level. MJIRl, Vol. 15, No.4, 219-225, 2002.
{"title":"TRANSFORMING GROWTH FACTOR ß2 UP-REGULATES GM-CSF GENE IN HUMAN BLADDER CARCINOMA CELL LINE HTB 5637","authors":"Z. S. Soheyli, B. Goliaei","doi":"10.1042/bst028a341a","DOIUrl":"https://doi.org/10.1042/bst028a341a","url":null,"abstract":"Transforming growth factor betas are multifunctional polypeptides in the cytokine superfamily. They have a growth inhibitory role on hemopoietic pro genitor cells in semisolid colony assay as well as in long-term bone-marrow cul ture. TGF -�2 represses stromal cells, stem cell factor gene transcription, and de creases the stability of c-kit transcripts in hemopoietic cells. TGF-� also modu lates GM-CSF production from human lymphocytes. The present study reveals the TGF-�2 role in production of GM-CSF in HTB 5637, human bladder carci noma cell line. HTB 5637 cells were treated with 5 ng/mL of human TGF -�?' viable cells were counted and GM-CSF concentration was determined, No anti proliferate activity of TGF-�2 on HTB 5637 cell line was observed. Biological assay showed increased levels of GM-CSF in the supernatant of cultured cells. However this increase was lower than that expected from ELISA. Since TGF-� may be an active suppressor factor regulating hemopoiesis, it seems that some inhibitory factor(s) may be produced (increased) in response to TGF-�2 treat ment. It has been shown that GM-CSF mRNA content from HTB 5637 cell line is very stable and this stabilization is translational dependent. Using Slot blot and Northern blot analysis, we determined that TGF-�2 upregulated GM-CSF gene expression in HTB 5637 cell line. The results suggest that TGF-�2 upregulates the production of GM-CSF gene at the transcriptional level. MJIRl, Vol. 15, No.4, 219-225, 2002.","PeriodicalId":22988,"journal":{"name":"The Medical Journal of The Islamic Republic of Iran","volume":"75 1","pages":"219-225"},"PeriodicalIF":0.0,"publicationDate":"2000-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86142513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1992-08-15DOI: 10.1016/0165-1161(92)91105-Z
H. Mozdarani
{"title":"ON THE EFFECTS OF ARA-A AND ARA-C ON X-RAY INDUCED DNA LESIONS IN NORMAL HUMAN AND A-T CELLS: SIMILARITIES AND DIFFERENCES.","authors":"H. Mozdarani","doi":"10.1016/0165-1161(92)91105-Z","DOIUrl":"https://doi.org/10.1016/0165-1161(92)91105-Z","url":null,"abstract":"","PeriodicalId":22988,"journal":{"name":"The Medical Journal of The Islamic Republic of Iran","volume":"13 1","pages":"123-127"},"PeriodicalIF":0.0,"publicationDate":"1992-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84995039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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