Transplantation proceedings最新文献_第9页

Discrepancy Between Pulmonary Artery Catheter and Co-Oximeter Value of Mixed Venous Oxygen Saturation After Graft Reperfusion During Living Donor Liver Transplantation 活体肝移植再灌注后肺动脉导管与共氧仪混合静脉血氧饱和度的差异。

IF 0.8 4区医学 Q4 IMMUNOLOGY

Transplantation proceedings

Pub Date : 2024-12-01 DOI: 10.1016/j.transproceed.2024.11.015

Yeonji Noh, Jeayoun Kim, Sooyeon Lee, Jisun Choi, Gaab Soo Kim

Background

Monitoring tissue oxygenation is critical in liver recipients. The pulmonary artery catheter (PAC) provides continuous monitoring of mixed venous oxygen saturation (SvO₂) using fiberoptic reflectance spectrophotometry. Despite the need for in vivo calibration during liver transplantation, recalibration guidelines are absent, and we frequently observed a significant discrepancy between PAC and reference co-oximeter SvO₂ values after graft reperfusion. This study aimed to assess the incidence and risk factors of a significant discrepancy after reperfusion during living donor liver transplantation.

Methods

This retrospective study included 54 recipients who underwent living donor liver transplantation at our institution between October 2021 and April 2022. A PAC was inserted, and in vivo calibration was conducted using the co-oximeter SvO₂ value. We defined a significant discrepancy as a drift was ≥ 3% at 1 hour after reperfusion. Logistic regression analysis was performed to determine the association between perioperative variables and the risk of significant discrepancy.

Results

PAC SvO₂ was higher than co-oximeter SvO₂ in 51 recipients. A significant discrepancy was observed in 37 recipients (68.5%). The risk of significant discrepancy decreased with a high preoperative hemoglobin concentration (odds ratio [OR] = 0.65 [0.47–0.91], P = .011) and a high arterial oxygen partial pressure (PaO₂) at 1 hour after reperfusion (OR = 0.96 [0.94–0.99], P = .004) but increased with a high baseline co-oximeter SvO₂ value (OR = 1.29 [1.05–1.59], P = .015).

Conclusions

PAC SvO₂ significantly drifted from the reference co-oximeter value in over two-thirds of recipients after reperfusion. Therefore, in vivo recalibration is required for the reliable measurement of PAC SvO₂ during living donor liver transplantation.

背景：监测组织氧合对肝脏受者至关重要。肺动脉导管（PAC）使用光纤反射分光光度法连续监测混合静脉氧饱和度（SvO2）。尽管在肝移植过程中需要进行体内校准，但缺乏再校准指南，我们经常观察到移植物再灌注后PAC和参考共氧仪SvO2值之间存在显着差异。本研究旨在评估活体肝移植再灌注后显著差异的发生率及危险因素。方法：本回顾性研究纳入了2021年10月至2022年4月期间在我院接受活体肝移植的54例受者。插入PAC，并使用共氧仪SvO2值进行体内校准。我们将显著差异定义为再灌注后1小时漂移≥3%。通过Logistic回归分析确定围手术期变量与显著差异风险的相关性。结果：51例患者PAC SvO2高于共血氧计SvO2。37例（68.5%）患者存在显著差异。术前较高的血红蛋白浓度（比值比[OR] = 0.65 [0.47-0.91], P = 0.011）和再灌注后1小时较高的动脉血氧分压（PaO2）（OR = 0.96 [0.94-0.99], P = 0.004）降低了显著差异的风险，但较高的基线共氧仪SvO2值（OR = 1.29 [1.05-1.59], P = 0.015）增加了显著差异的风险。结论：在再灌注后，超过三分之二的受者PAC SvO2明显偏离参考共氧仪值。因此，在活体供肝移植期间，需要在体内重新校准PAC SvO2的可靠测量。

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引用次数: 0

Current Status and Development of Rat DCD Renal Transplantation Model 大鼠 DCD 肾移植模型的现状与发展。

IF 0.8 4区医学 Q4 IMMUNOLOGY

Transplantation proceedings

Pub Date : 2024-12-01 DOI: 10.1016/j.transproceed.2024.11.004

Shang Xu, Zhilong Liu, Xinning Wang

Donation after Cardiac Death (DCD) is an important source of kidneys for kidney transplantation, but DCD kidney transplantation is associated with more complications, impacting the function of the transplanted kidney. Establishing a stable DCD kidney transplantation model in rats can help study the mechanisms of injury and complications prevention in DCD kidney transplantation. This review summarizes the current research status and progress in rat DCD kidney transplantation.

心脏死亡后捐献（DCD）是肾移植的重要肾源，但DCD肾移植并发症较多，影响移植肾的功能。建立稳定的 DCD 肾移植大鼠模型有助于研究 DCD 肾移植的损伤和并发症预防机制。本综述总结了大鼠 DCD 肾移植的研究现状和进展。

引用次数: 0

Comparison of 2 Different Types of Arterial and Biliary Anastomoses While Preserving the Gastroduodenal Artery: A Retrospective Study 保留胃十二指肠动脉的两种不同类型动脉胆道吻合术的比较：回顾性研究。

IF 0.8 4区医学 Q4 IMMUNOLOGY

Transplantation proceedings

Pub Date : 2024-12-01 DOI: 10.1016/j.transproceed.2024.10.040

Yafei Guo, Zebin Zhu, Wei Wu, Hao Zheng, Xiaodong Yuan, Can Qi, Zhijun Xu, Xuefeng Li, Ning Wang, Jiwei Qin, Ruipeng Song, Jizhou Wang, Dalong Yin, Lianxin Liu, Shugeng Zhang, Björn Nashan

Objective

Here we investigated 2 different arterial reconstruction procedures with preservation of the gastroduodenal artery (GDA) in orthotopic liver transplantation (OLT).

Methods

A retrospective cohort study was conducted. The clinicopathological data of 40 pairs of donors and recipients who underwent OLT from May 2018 to August 2023 were analyzed. The GDA of all recipients was preserved during the hepatic arteries reconstruction, which were divided into 2 groups according to different anastomosis locations, namely the end-to-side group (E-S group, n = 17, Branch-patch between the common hepatic and the gastroduodenal artery) and end-to-end group (E-E group, n = 23, between common hepatic and proper hepatic).

Results

Demographics did not differ between both groups (P > .05). The warm ischemia time (WIT) was numerically longer in the E-S group (403.3 ± 103.3 vs 195.2 ± 35.8 s; P < .001), but the cold ischemia time (CIT) was numerically shorter in the E-S group (279.2 ± 74.7 vs 325.2 ± 15.1 min; P = .023). The time of artery reconstruction and duration of surgery was numerically shorter in the E-S group (21.0 ± 3.6 vs 51.0 ± 9.1 min, P < .001; 326.0 ± 103.4 vs 422.7 ± 24.6 min, P = .001). The E-S group had a lower resistance index (RI) (0.5 ± 0.3 vs 0.6 ± 0.1; P = .049).

Conclusion

Both groups showed no differences in artery and biliary complications. GDA preservation meets the anatomical and physiological requirements. E-S is superior to E-E by the time of hepatic artery reconstruction and RI.

目的：探讨原位肝移植（OLT）中保留胃十二指肠动脉（GDA）的两种不同的动脉重建方法。方法：采用回顾性队列研究。分析2018年5月至2023年8月40对肾移植供体和受体的临床病理资料。所有受术者在肝动脉重建过程中均保留GDA，根据吻合位置的不同分为2组，端侧组（E-S组，n = 17，肝总动脉与胃十二指肠动脉之间的分支贴片）和端端组（E-E组，n = 23，肝总动脉与肝原动脉之间的分支贴片）。结果：两组人口统计学差异无统计学意义（P < 0.05）。E-S组大鼠热缺血时间（WIT）数值上更长（403.3±103.3 vs 195.2±35.8 s）；P < 0.001)，但E-S组的冷缺血时间（CIT）较E-S组短（279.2±74.7 vs 325.2±15.1 min）；P = .023)。E-S组动脉重建时间和手术时间较E-S组短(21.0±3.6 min vs 51.0±9.1 min, P < 0.001；326.0±103.4 vs 422.7±24.6 min, P = .001)。E-S组耐药指数（RI）较低（0.5±0.3 vs 0.6±0.1）；P = .049)。结论：两组动脉及胆道并发症无明显差异。GDA保存符合解剖和生理要求。E-S在肝动脉重建及RI时间上优于E-E。

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引用次数: 0

Impact of Preprocurement Cardiac Arrest in Brain-Dead Donors on the Outcome of Pancreas Transplantation 脑死亡供体获取前心脏骤停对胰腺移植结果的影响。

IF 0.8 4区医学 Q4 IMMUNOLOGY

Transplantation proceedings

Pub Date : 2024-12-01 DOI: 10.1016/j.transproceed.2024.11.011

Minyan Zheng , Yue Wu , Jie Xiang , Li Wang , Zhiwei Li , Feng Gao

Background

This study aimed to determine the risk factors and whether cardiac arrest (CA) in brain-death donors (DBD) could affect pancreas transplantation outcomes.

Methods

We analyzed data from the Scientific Registry of Transplant Recipients (2000-2020). The study included 21,499 pancreas transplantations, divided into CA-DBD and noCA-DBD groups based on whether the DBD had a history of CA.

Results

There were 1129 CA-DBD (5.3%) transplantations. The principal donor death cause for both groups was head trauma. Graft and patient survival rates were similar in both groups. CA time (CAT) was a risk factor for pancreatic graft survival in the univariate analysis (hazard ratio [HR], 1.05; 95% confidence interval [CI], 1.03-1.08; P = .010) and multivariate Cox regression model (HR, 1.03; 95% CI, 1.02-1.04; P =.015). Pancreas graft survival in those with CAT ≥30 minutes was significantly lower than in those with CAT <30 minutes and the noCA-DBD group (log-rank P = .018 and P = .014, respectively), which were comparable (log-rank P = .711). No relationships were found among the various transplantation types.

Conclusions

CA in donors did not affect the pancreatic graft prognosis. However, pancreatic donors with CAT ≥30 minutes should be meticulously evaluated.

背景：本研究旨在确定脑死亡供体（DBD）的危险因素和心脏骤停（CA）是否会影响胰腺移植结果。方法：我们分析了移植受者科学登记处（2000-2020）的数据。本研究纳入21499例胰腺移植，根据患者是否有ca病史分为CA-DBD组和noCA-DBD组。结果：CA-DBD移植1129例（5.3%）。两组的主要供体死亡原因都是头部创伤。两组的移植物和患者存活率相似。在单因素分析中，CA时间（CAT）是胰腺移植存活的危险因素(风险比[HR]， 1.05；95%置信区间[CI]， 1.03-1.08；P = 0.010)和多因素Cox回归模型(HR, 1.03；95% ci, 1.02-1.04；P = .015)。CAT≥30分钟的胰腺移植存活率明显低于CAT患者。结论：供体CA不影响胰腺移植预后。然而，CAT≥30分钟的胰腺供者应仔细评估。

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引用次数: 0

Clinical Outcomes in Delayed Sternal Closure in Lung Transplantation 肺移植延迟胸骨闭合的临床结果。

IF 0.8 4区医学 Q4 IMMUNOLOGY

Transplantation proceedings

Pub Date : 2024-12-01 DOI: 10.1016/j.transproceed.2024.10.044

Arvind Bakthavatsalam , Billanna Hwang , Michael S. Mulligan

Introduction

Lung transplantation (LTx) is a critical treatment for end-stage pulmonary diseases. Delayed sternal closure (DSC) has been noted in cardiac surgery for several decades and is a viable strategy for patients who are hemodynamically unstable, at high risk for postoperative bleeding, and/or require prolonged cardiopulmonary bypass. However, DSC use and its effects on LTx clinical outcomes are not well-defined.

Methods

A retrospective analysis was performed on patients who had undergone LTx between January 1, 2010, and October 1, 2018, at the University of Washington. Patients under the age of 18, undergoing repeat LTx, other solid organ transplantation, and/or had prior major cardiothoracic surgery were excluded. Patients were classified into 2 categories: DSC and primary sternal closure (PSC) groups. We examined hospital length of stay (LOS), intensive care unit (ICU) LOS, duration on the ventilator, and overall survival.

Results

A total of 370 patients were identified, with 350 patients in the PSC group and 20 patients in the DSC group. The median hospital LOS, ICU LOS and duration on the ventilator in the DSC group were 23.0 ± 3.8, 16.0 ± 3.6, and 9.0 ± 3.5 compared with 15.0 ± 0.8, 5.0 ± 0.5, and 1.0 ± 0.3 in the PSC group, respectively (All P < .01). Kaplan–Meier analysis revealed that, at 1, 3, and 5 years, the DSC group had a survival of 75.0%, 60.0%, and 55.0%, and the PSC group had a survival of 91.4%, 82.6% and 75.1% respectively (P = .019).

Conclusion

DSC is not uncommon in LTx and patients who do require DSC had a longer duration on the ventilator, ICU LOS, and hospital LOS. Additionally, the DSC group had a worse survival compared with the PSC group. DSC is unlikely to be the sole factor influencing worse survival, but may be a marker of underlying patient factors that negatively affect survival.

肺移植（LTx）是终末期肺部疾病的重要治疗方法。延迟胸骨闭锁术（DSC）在心脏外科手术中已经被注意了几十年，对于血液动力学不稳定、术后出血高风险和/或需要长时间体外循环的患者来说，这是一种可行的策略。然而，DSC的使用及其对LTx临床结果的影响尚不明确。方法：对2010年1月1日至2018年10月1日在华盛顿大学接受LTx治疗的患者进行回顾性分析。年龄在18岁以下、正在接受重复LTx、其他实体器官移植和/或之前有过重大心胸手术的患者被排除在外。将患者分为DSC组和PSC组。我们检查了住院时间（LOS）、重症监护病房（ICU） LOS、呼吸机持续时间和总生存期。结果：共发现370例患者，其中PSC组350例，DSC组20例。DSC组住院LOS、ICU LOS和呼吸机使用时间中位数分别为23.0±3.8、16.0±3.6和9.0±3.5，PSC组为15.0±0.8、5.0±0.5和1.0±0.3 （P均< 0.01）。Kaplan-Meier分析显示，在1、3和5年时，DSC组的生存率分别为75.0%、60.0%和55.0%，PSC组的生存率分别为91.4%、82.6%和75.1% （P = 0.019）。结论：DSC在LTx中并不罕见，需要DSC的患者在呼吸机、ICU LOS和医院LOS上的持续时间更长。此外，与PSC组相比，DSC组的生存率更差。DSC不太可能是影响生存差的唯一因素，但可能是潜在的患者因素对生存产生负面影响的一个标志。

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引用次数: 0

Incidence and Risk Factors for Clinically Significant Oropharyngeal Dysphagia After Lung Transplantation 肺移植术后临床显著口咽吞咽困难的发生率及危险因素。

IF 0.8 4区医学 Q4 IMMUNOLOGY

Transplantation proceedings

Pub Date : 2024-12-01 DOI: 10.1016/j.transproceed.2024.11.003

Jacques Neelankavil , Reed Harvey , Jure Marijic , Michael Tan , Lorraine Lubin , Ali Salehi , Theodora Wingert , Tristan Grogan , David Sayah , Abbas Ardehali

Aspiration is one of many risk factors for chronic lung allograft dysfunction, which continues to limit survival in lung transplant recipients. Oropharyngeal dysphagia (OPD) occurring after lung transplant surgery increases the risk of antegrade aspiration. While the incidence and risk factors for OPD have been well described in cardiac surgery, there is less known in the lung transplant population. The aim of this retrospective, single-center study was to determine the incidence of clinically significant OPD in lung transplant recipients and to identify patient and procedural risk factors for OPD in this population. A total of 158 lung transplant recipients who underwent postoperative fiberoptic endoscopic evaluation of swallowing (FEES) were included in the study. The incidence of OPD was 67.1% (106/158) with 29.8% (47) having a complete absence of swallowing function. Female sex, cardiopulmonary bypass, duration of intubation, and an increased number of transesophageal echocardiography (TEE) clips were associated with OPD. The incidence of OPD in this study is consistent with the limited data available in lung transplant literature, and higher than that of general cardiac surgery. An increased number of acquired TEE clips is a novel OPD risk factor in this population and a modifiable target for future intervention.

误吸是慢性同种异体肺移植功能障碍的众多危险因素之一，它继续限制肺移植受者的生存。肺移植术后发生的口咽吞咽困难（OPD）增加了顺行误吸的风险。虽然OPD的发病率和危险因素在心脏手术中已经有了很好的描述，但在肺移植人群中却知之甚少。这项回顾性、单中心研究的目的是确定肺移植受者临床显著性OPD的发生率，并确定该人群中OPD的患者和手术危险因素。共有158名接受术后纤维内镜吞咽评估（FEES）的肺移植受者被纳入研究。OPD的发生率为67.1%(106/158)，其中29.8%（47）患者完全没有吞咽功能。女性、体外循环、插管时间和经食管超声心动图（TEE）夹次数增加与OPD相关。本研究中OPD的发生率与肺移植文献中有限的数据一致，且高于普通心脏手术。获得性TEE夹数量的增加是该人群中新的OPD风险因素，也是未来干预的可修改目标。

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引用次数: 0

Effect of Parathyroidectomy After Renal Transplantation on Grafted Kidney Function Within One Year: A Meta-Analysis 肾移植术后甲状旁腺切除术对一年内移植肾功能的影响：一项荟萃分析。

IF 0.8 4区医学 Q4 IMMUNOLOGY

Transplantation proceedings

Pub Date : 2024-12-01 DOI: 10.1016/j.transproceed.2024.11.019

Fu Yan , Zhou Sun , Guofu Liang , Chao Liu , Yulin Niu

Objective

Secondary hyperparathyroidism (sHPT) is a common symptom of chronic kidney disease (CKD), and at the time of transplantation, more than two-thirds of patients with end-stage renal disease have secondary hyperparathyroidism. After kidney transplantation, parathyroid function is normalized in some kidney transplant recipients, but up to 50% of kidney transplant recipients develop tertiary hyperparathyroidism (tHPT) 1 year after kidney transplantation. The effect of parathyroidectomy on the grafts is currently unclear; thus, we conducted a meta-analysis of relevant studies to evaluate changes in graft function 1 year after parathyroidectomy, aiming to assess the procedure's safety in renal transplant recipients.

Methods

A thorough exploration was conducted across Embase, PubMed, Web of Science, and the Cochrane Library databases to gather pertinent literature spanning from January 1, 2000, to December 31, 2023. The search criteria encompassed terms such as “kidney transplantation,” “parathyroidectomy,” and “hyperparathyroidism.”

Results

Twelve studies were scrutinized to assess alterations in graft functionality at 1, 3, 6, and 12 months postparathyroidectomy. The meta-analysis unveiled a notable decline in overall glomerular filtration rate and a concurrent elevation in serum creatinine 1 year postparathyroidectomy, signifying an impairment in graft function compared to the preoperative phase. Significant heterogeneity was observed among the studies.

Conclusion

Following parathyroidectomy in renal transplant recipients 1 year postsurgery, calcium and parathyroid hormone levels normalized. Nonetheless, there was evident impairment in graft function and an elevated risk of graft loss. Hence, the safety of parathyroidectomy in patients with secondary hyperparathyroidism postrenal transplantation necessitates meticulous consideration.

目的：继发性甲状旁腺功能亢进（sHPT）是慢性肾脏疾病（CKD）的常见症状，在移植时，超过三分之二的终末期肾脏疾病患者有继发性甲状旁腺功能亢进。肾移植后，一些肾移植受者的甲状旁腺功能恢复正常，但高达50%的肾移植受者在肾移植后1年发生三级甲状旁腺功能亢进（tHPT）。甲状旁腺切除术对移植物的影响目前尚不清楚；因此，我们对相关研究进行了荟萃分析，以评估甲状旁腺切除术后1年移植物功能的变化，旨在评估该手术在肾移植受者中的安全性。方法：全面检索Embase、PubMed、Web of Science和Cochrane Library数据库，收集2000年1月1日至2023年12月31日的相关文献。搜索标准包括“肾移植”、“甲状旁腺切除术”和“甲状旁腺功能亢进”等术语。“结果：12项研究被仔细审查，以评估甲状旁腺切除术后1、3、6和12个月移植物功能的改变。荟萃分析显示，甲状旁腺切除术后1年，总体肾小球滤过率显著下降，血清肌酐升高，与术前相比，移植物功能受损。研究之间存在显著的异质性。结论：肾移植术后1年行甲状旁腺切除术后，钙和甲状旁腺激素水平恢复正常。尽管如此，移植物功能明显受损，移植物丧失的风险升高。因此，肾移植后继发性甲状旁腺功能亢进患者行甲状旁腺切除术的安全性需要慎重考虑。

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引用次数: 0

Liver Transplantation in the Philippines: Light at the End of the Tunnel 菲律宾的肝脏移植手术：隧道尽头的曙光

IF 0.8 4区医学 Q4 IMMUNOLOGY

Transplantation proceedings

Pub Date : 2024-12-01 DOI: 10.1016/j.transproceed.2024.11.009

Siegfredo R. Paloyo , Ferri P. David-Paloyo , Rose Marie R. Liquete , Enrique T. Ona

End-stage liver disease is arguably one of the leading burdensome diseases among developing countries such as the Philippines. Although liver transplantation is considered the treatment of choice for decompensated cirrhosis, the establishment of a robust transplant program locally has been protracted as numerous obstacles continue to plague our transplant landscape. Issues on cost, options of having the transplant done overseas, and low rates of deceased donation are some of the difficulties that hamper our program's progress and development. In this study, we aim to present our experience and discuss hurdles, describing the current status of liver transplantation in the country.

Herein we report our initial 68 liver transplants covering 35 years. A retrospective review was performed including all transplants done in 4 active transplant centers from August 1988 to December 2023. Recipients were mostly male patients (69%) with hepatitis B as the most common (32%) primary liver disease. Deceased donor liver transplant was more common (65%) with recipients having a median Model for End-Stage Liver Disease (MELD) score of 22. Among living donor liver transplants, 58% of the grafts were from the left lateral segment. Outcomes can be divided into two eras, with the first era (1988-2010) having a 1-year graft survival of only 25%. The second era (2011-present) was marked with surgical refinements and advancing knowledge, improving 1-year graft survival to 64% and 67% for adult and pediatric transplants, respectively. Indeed, the establishment of a liver transplant program requires a team approach and most fundamentally, full training in liver transplantation and the management of its complications. Optimal patient selection remains essential in achieving excellent survival outcomes. Last, Filipinos with liver cirrhosis now have an opportunity for a significant improvement in their quality of life by undergoing transplantation in their native country.

终末期肝病可以说是菲律宾等发展中国家的主要负担疾病之一。虽然肝移植被认为是治疗失代偿期肝硬化的首选方法，但在当地建立健全的移植项目却一直旷日持久，因为众多障碍一直困扰着我们的移植事业。成本问题、选择在海外进行移植以及死者捐献率低都是阻碍我们项目进展和发展的一些困难。在本研究中，我们旨在介绍我们的经验，讨论存在的障碍，描述我国肝移植的现状。在此，我们报告了我们最初的 68 例肝移植手术，时间跨度长达 35 年。我们对 1988 年 8 月至 2023 年 12 月期间在 4 个活跃的移植中心进行的所有移植手术进行了回顾性审查。受者多为男性患者（69%），乙型肝炎是最常见的原发性肝病（32%）。死亡供体肝移植更常见（65%），受者的终末期肝病模型（MELD）中位数为 22 分。在活体肝移植中，58%的移植物来自左侧肝段。结果可分为两个时代，第一个时代（1988-2010年）的1年移植物存活率仅为25%。第二个时代（2011年至今）的特点是手术的改进和知识的进步，成人和儿童移植手术的1年存活率分别提高到64%和67%。事实上，肝移植项目的建立需要团队合作，最根本的是需要接受肝移植及其并发症处理方面的全面培训。最佳的患者选择仍然是取得良好生存结果的关键。最后，患有肝硬化的菲律宾人现在有机会在自己的祖国接受移植手术，从而显著改善生活质量。

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引用次数: 0

Motivational Factors Affecting Body and Organ Donation in China Identified Based on Grounded Theory 基于基础理论识别影响中国遗体和器官捐献的动机因素。

IF 0.8 4区医学 Q4 IMMUNOLOGY

Transplantation proceedings

Pub Date : 2024-12-01 DOI: 10.1016/j.transproceed.2024.11.005

Yunfeng Wang , Ping Dong , Yukun Zhou , Shanshan Gao , Luqing Zhang

Background

In recent decades, China has experienced a shortage of donated bodies and organs for dissection during medical education and clinical transplantation. This study investigated and analyzed motivational factors affecting body and organ donation in China.

Methodology

Thirty-three body and organ donation volunteers were interviewed in-depth, and their donation motivations were investigated. The data were decomposed, detected, compared, conceptualized, and categorized using open, correlation, and selective coding based on grounded theory.

Results

The results were used to construct a model that elucidates the foundation of body and organ donation based on “humanistic medical care” and “death in existentialism” as driving forces for body and organ donors.

Conclusions

This study provides new theoretical perspectives that contribute to interpreting behavior related to body and organ donation and offers a theoretical basis for related policymaking and promotional work.

背景：近几十年来，中国医学教育和临床移植过程中用于解剖的捐献遗体和器官出现短缺。本研究对影响中国遗体和器官捐献的动机因素进行了调查和分析：对 33 名遗体和器官捐献志愿者进行了深入访谈，调查了他们的捐献动机。根据基础理论，采用开放编码、相关编码和选择编码对数据进行分解、检测、比较、概念化和分类：结果：研究结果被用于构建一个模型，该模型以 "人文医疗 "和 "存在主义中的死亡 "作为遗体和器官捐献者的驱动力，阐明了遗体和器官捐献的基础：本研究提供了新的理论视角，有助于解释与遗体和器官捐献相关的行为，并为相关政策制定和宣传工作提供了理论依据。

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引用次数: 0

Diosgenin Mediated JAK/STAT Signal Channel Improving SD Rat Renal Ischemia-Reperfusion 薯蓣皂苷介导的 JAK/STAT 信号通道改善 SD 大鼠肾缺血再灌注。

IF 0.8 4区医学 Q4 IMMUNOLOGY

Transplantation proceedings

Pub Date : 2024-12-01 DOI: 10.1016/j.transproceed.2024.11.007

Jinran Yang, Xinchang Li, Chengmei Long, Youfu Zhang, Hua Yang, Tongchang Chen, Xuyang Wang, Xiaomei Huang

Objective

To explore the mechanism of action of diosgenin and JAK/STAT in SD rat renal ischemia-reperfusion (I/R) injury.

Method

A renal I/R injury model was established, rat was divided into four groups: control group, sham surgery group, I/R model group, and diosgenin pretreatment group. 48 hours after the completion of modeling, blood was collected for testing of SCr, BUN, and Kim-1. After blood collection, bilateral kidneys were cut and stored in liquid nitrogen for HE staining. Western blot was used to detect the protein expression levels of JAK and STAT in each group. Compare and analyze the differences in JAK/STAT expression levels among different groups.

Result

Compared with the I/R model group, the Scr, Bun, and Kim-1 concentrations of the three treatment groups were significantly lower, the other three treatment groups showed significant improvement in symptoms of renal vacuolar degeneration and interstitial edema in rats, the expression levels of JAK and STAT in the three groups were significantly lower, while the difference between the three groups was not significant.

目的探讨薯蓣皂苷与JAK/STAT在SD大鼠肾缺血再灌注损伤中的作用机制：方法：建立SD大鼠肾缺血再灌注损伤模型，将大鼠分为四组：对照组、假手术组、I/R模型组和地奥司宁预处理组。模型制作完成 48 小时后，采血检测 SCr、BUN 和 Kim-1。采血后，切取双侧肾脏并保存在液氮中进行 HE 染色。用 Western blot 检测各组 JAK 和 STAT 的蛋白表达水平。比较并分析不同组间 JAK/STAT 表达水平的差异：与I/R模型组相比，三个治疗组的Scr、Bun和Kim-1浓度明显降低，其他三个治疗组大鼠肾空泡变性和间质水肿症状明显改善，三个治疗组的JAK和STAT表达水平明显降低，三组间差异无显著性。

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引用次数: 0