Intracerebral hemorrhages (ICHs) are devastating neurological events frequently resulting in a serious negative prognosis. The exact physiological and disease processes involved in ICHs are complex, but are thought to involve microRNAs (miRNAs), 22 nucleotide small noncoding RNAs that control a variety of normal physiological and disease processes. In this study, we show that a miRNA, miR-34a, regulates BDNF in a model of ICH injury. In particular, we assessed the impact of AM34a, an inhibitor of miR-34a, on the toxicity of thrombin-induced apoptosis and on BDNF-mediated signaling. We investigated the increased expression of miR-34a after an ICH-induced thrombin toxicity injury using a real-time polymerase chain reaction (RT-PCR) and evaluated miR-34a as a therapeutic target. Apoptosis was confirmed by 4',6-diamidino-2-phenylindole using terminal deoxynucleotidyl transferase-mediated digoxigenindUTP- biotin nick-end labeling (TUNEL). The number of apoptotic cells detected by TUNEL after ICH injury was decreased by AM34a. Additionally, the ICH injury model treated with AM34a had a significantly lower caspase-3 level. We performed western blot analyses for BDNF, phosphorylated Akt, and phosphorylated ERK. The levels of BDNF were significantly higher in samples treated with AM34a. Furthermore, the level of phosphorylated Akt and phosphorylated ERK were significantly higher under AM34a. In conclusion, we demonstrated a distinct miRNA expression pattern after an in vitro ICH injury model, and modulation of this pattern can have therapeutic potential. miR-34a antagomir reduced cell death and enhanced neurological recovery by activating the BDNF pro-survival pathway. This suggests that inhibiting miR-34a might be a potential therapeutic target in ICH.
{"title":"MicroRNA-34a regulates brain-derived neurotrophic factor in an intracerebral hemorrhage model","authors":"Jin Hee Kim, Jae-Sun Choi","doi":"10.3906/BIY-1606-16","DOIUrl":"https://doi.org/10.3906/BIY-1606-16","url":null,"abstract":"Intracerebral hemorrhages (ICHs) are devastating neurological events frequently resulting in a serious negative prognosis. The exact physiological and disease processes involved in ICHs are complex, but are thought to involve microRNAs (miRNAs), 22 nucleotide small noncoding RNAs that control a variety of normal physiological and disease processes. In this study, we show that a miRNA, miR-34a, regulates BDNF in a model of ICH injury. In particular, we assessed the impact of AM34a, an inhibitor of miR-34a, on the toxicity of thrombin-induced apoptosis and on BDNF-mediated signaling. We investigated the increased expression of miR-34a after an ICH-induced thrombin toxicity injury using a real-time polymerase chain reaction (RT-PCR) and evaluated miR-34a as a therapeutic target. Apoptosis was confirmed by 4',6-diamidino-2-phenylindole using terminal deoxynucleotidyl transferase-mediated digoxigenindUTP- biotin nick-end labeling (TUNEL). The number of apoptotic cells detected by TUNEL after ICH injury was decreased by AM34a. Additionally, the ICH injury model treated with AM34a had a significantly lower caspase-3 level. We performed western blot analyses for BDNF, phosphorylated Akt, and phosphorylated ERK. The levels of BDNF were significantly higher in samples treated with AM34a. Furthermore, the level of phosphorylated Akt and phosphorylated ERK were significantly higher under AM34a. In conclusion, we demonstrated a distinct miRNA expression pattern after an in vitro ICH injury model, and modulation of this pattern can have therapeutic potential. miR-34a antagomir reduced cell death and enhanced neurological recovery by activating the BDNF pro-survival pathway. This suggests that inhibiting miR-34a might be a potential therapeutic target in ICH.","PeriodicalId":23358,"journal":{"name":"Turkish Journal of Biology","volume":"41 1","pages":"249-255"},"PeriodicalIF":2.2,"publicationDate":"2017-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3906/BIY-1606-16","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47218002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mohsen Rezvani, G. Najafpour, M. Mohammadi, H. Zare
A simple and robust single enzyme biosensor was fabricated for triglyceride (TG) detection. Graphite rods were modified with activated carbon (AC) and used as support for lipase immobilization. Chitosan (CHIT) was eventually used to create film on the bioelectrode and retain the immobilized enzyme. To extend the linear range of TG detection, AC was functionalized with carboxyl and then amine groups (AAC) to enhance the isoelectric point of AC. The constructed graphite/AAC/lipase /CHIT bioelectrode was characterized using cyclic voltammetry and field emission scanning electron microscopy (FE-SEM). The accuracy of the developed biosensor was assessed through determination of different concentrations of tributyrin (TB) in buffer solution. Linear responses were found for TB concentration in the range of 50 to 350 mg dL-1 with a detection limit of 9.9 mg dL-1. The biosensor showed good sensitivity of 0.16 μA mg-1 dL. The detected level of TG in several human serum specimens using the constructed biosensor was in good agreement with the results of an automatic biochemical analyzer. The fabricated biosensor was not affected by a number of human serum materials and showed a strong anti-interference ability. The relative standard deviation in reproducibility and repeatability tests was 3.46% and 2.94%, respectively.
{"title":"Amperometric biosensor for detection of triglyceride tributyrinbased on zero point charge of activated carbon","authors":"Mohsen Rezvani, G. Najafpour, M. Mohammadi, H. Zare","doi":"10.3906/BIY-1607-24","DOIUrl":"https://doi.org/10.3906/BIY-1607-24","url":null,"abstract":"A simple and robust single enzyme biosensor was fabricated for triglyceride (TG) detection. Graphite rods were modified with activated carbon (AC) and used as support for lipase immobilization. Chitosan (CHIT) was eventually used to create film on the bioelectrode and retain the immobilized enzyme. To extend the linear range of TG detection, AC was functionalized with carboxyl and then amine groups (AAC) to enhance the isoelectric point of AC. The constructed graphite/AAC/lipase /CHIT bioelectrode was characterized using cyclic voltammetry and field emission scanning electron microscopy (FE-SEM). The accuracy of the developed biosensor was assessed through determination of different concentrations of tributyrin (TB) in buffer solution. Linear responses were found for TB concentration in the range of 50 to 350 mg dL-1 with a detection limit of 9.9 mg dL-1. The biosensor showed good sensitivity of 0.16 μA mg-1 dL. The detected level of TG in several human serum specimens using the constructed biosensor was in good agreement with the results of an automatic biochemical analyzer. The fabricated biosensor was not affected by a number of human serum materials and showed a strong anti-interference ability. The relative standard deviation in reproducibility and repeatability tests was 3.46% and 2.94%, respectively.","PeriodicalId":23358,"journal":{"name":"Turkish Journal of Biology","volume":"41 1","pages":"268-277"},"PeriodicalIF":2.2,"publicationDate":"2017-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3906/BIY-1607-24","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48401816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In this study, a novel laccase gene, named as Cplcc1, and its corresponding cDNA were isolated and characterized from the Coriolopsis polyzona MUCL 38443 strain. The Cplcc1 gene consists of a 1563-bp open reading frame encoding a protein of 520 amino acids with a 20-residue putative signal peptide. The size of the Cplcc1 gene is 2106 bp and it contains ten introns and five potential N-glycosylation sites. Additionally, the isolated full-length Cplcc1 cDNA was successfully expressed in Pichia pastoris. The heterologous expression conditions were also optimized and the highest activity value increased to 800 U L-1 with 1.5% methanol, 0.8 mM CuSO4, and 0.6% L-alanine supplementation. The recombinant laccase was partially purified and the molecular weight was found as approximately 54 kDa. The maximum oxidation activity was observed for 2,2-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) at pH 3.0. The optimal temperature was found as 70 °C. On the other hand, at 30 °C, the enzyme was stable for more than a week and its half-life was longer than 8 h. The Km, Vmax, kcat, and kcat Km-1 values of the recombinant laccase were identified as 0.137 mM, 288.6 μmol min-1 L-1, 5.73 x 105 min-1, and 4.18 x 106 min-1 mM-1, respectively. Sodium azide, L-cysteine, and SDS were found as usual inhibitors.
本研究从多带Coriolopsis polyzona MUCL 38443菌株中分离到一种新的漆酶基因Cplcc1及其cDNA，并对其进行了鉴定。Cplcc1基因由一个1563 bp的开放阅读框组成，编码520个氨基酸的蛋白质和20个残基的信号肽。Cplcc1基因全长2106 bp，包含10个内含子和5个潜在的n -糖基化位点。此外，分离的Cplcc1全长cDNA在毕赤酵母中成功表达。在1.5%甲醇、0.8 mM CuSO4和0.6% l -丙氨酸的条件下，该菌株的最高表达活性为800 U L-1。重组漆酶经部分纯化，分子量约为54 kDa。2,2-氮唑-(3-乙基苯并噻唑-6-磺酸)(ABTS)的氧化活性在pH 3.0时达到最大。最佳温度为70℃。在30℃条件下，酶稳定时间超过1周，半衰期大于8 h。重组漆酶的Km、Vmax、kcat和kcat Km-1分别为0.137 mM、288.6 μmol min-1 L-1、5.73 × 105 min-1和4.18 × 106 min-1 mM-1。叠氮化钠、l -半胱氨酸和SDS是常见的抑制剂。
{"title":"Heterologous expression and characterization of a high redox potential laccase from Coriolopsis polyzona MUCL 38443","authors":"O. Pinar, Candan Tamerler Behar, A. Karataş","doi":"10.3906/BIY-1605-51","DOIUrl":"https://doi.org/10.3906/BIY-1605-51","url":null,"abstract":"In this study, a novel laccase gene, named as Cplcc1, and its corresponding cDNA were isolated and characterized from the Coriolopsis polyzona MUCL 38443 strain. The Cplcc1 gene consists of a 1563-bp open reading frame encoding a protein of 520 amino acids with a 20-residue putative signal peptide. The size of the Cplcc1 gene is 2106 bp and it contains ten introns and five potential N-glycosylation sites. Additionally, the isolated full-length Cplcc1 cDNA was successfully expressed in Pichia pastoris. The heterologous expression conditions were also optimized and the highest activity value increased to 800 U L-1 with 1.5% methanol, 0.8 mM CuSO4, and 0.6% L-alanine supplementation. The recombinant laccase was partially purified and the molecular weight was found as approximately 54 kDa. The maximum oxidation activity was observed for 2,2-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) at pH 3.0. The optimal temperature was found as 70 °C. On the other hand, at 30 °C, the enzyme was stable for more than a week and its half-life was longer than 8 h. The Km, Vmax, kcat, and kcat Km-1 values of the recombinant laccase were identified as 0.137 mM, 288.6 μmol min-1 L-1, 5.73 x 105 min-1, and 4.18 x 106 min-1 mM-1, respectively. Sodium azide, L-cysteine, and SDS were found as usual inhibitors.","PeriodicalId":23358,"journal":{"name":"Turkish Journal of Biology","volume":"41 1","pages":"278-291"},"PeriodicalIF":2.2,"publicationDate":"2017-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3906/BIY-1605-51","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70173556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Kizildogan, G. Jaccard, Alper Mutlu, Ibrahim Sertdemir, G. Özcengiz
An industrial clavulanic acid (CA) overproducer Streptomyces clavuligerus strain, namely DEPA, was engineered to further enhance its CA production. Single or multiple copies of ccaR, claR (pathway-specific activators), and cas2 (CA synthase) genes under the control of different promoters were introduced into this strain. CA titers of the resulting recombinants were analyzed by HPLC in a dynamic fashion and compared to the vector-only controls and a wild-type strain of S. clavuligerus while their growth was monitored throughout fermentation. The addition of an extra copy of ccaR, under control of its own promoter or constitutive ermE* promoter (PermE*), led to 7.6- and 2.3-fold increased volumetric levels of CA in respective recombinants, namely the AK9 and ID3 strains. Its highly stable multicopy expression by the glpF promoter (PglpF) provided up to 25.9-fold enhanced volumetric CA titers in the respective recombinant, IDG3. claR expression controlled with its own promoter or ermE* and glpF-mediated amplification in an industrial strain brought about only about 1.2-fold increase in the volumetric CA titers. An extra copy of cas2 integration with PermE* into the S. clavuligerus DEPA genome led to 3.8-fold higher volumetric CA production by GV61. Conclusively, multicopy expression of ccaR under PglpF can result in significantly improved industrial high-titer CA producers.
{"title":"Genetic engineering of an industrial strain of Streptomyces clavuligerus for further enhancement of clavulanic acid production","authors":"A. Kizildogan, G. Jaccard, Alper Mutlu, Ibrahim Sertdemir, G. Özcengiz","doi":"10.3906/BIY-1608-17","DOIUrl":"https://doi.org/10.3906/BIY-1608-17","url":null,"abstract":"An industrial clavulanic acid (CA) overproducer Streptomyces clavuligerus strain, namely DEPA, was engineered to further enhance its CA production. Single or multiple copies of ccaR, claR (pathway-specific activators), and cas2 (CA synthase) genes under the control of different promoters were introduced into this strain. CA titers of the resulting recombinants were analyzed by HPLC in a dynamic fashion and compared to the vector-only controls and a wild-type strain of S. clavuligerus while their growth was monitored throughout fermentation. The addition of an extra copy of ccaR, under control of its own promoter or constitutive ermE* promoter (PermE*), led to 7.6- and 2.3-fold increased volumetric levels of CA in respective recombinants, namely the AK9 and ID3 strains. Its highly stable multicopy expression by the glpF promoter (PglpF) provided up to 25.9-fold enhanced volumetric CA titers in the respective recombinant, IDG3. claR expression controlled with its own promoter or ermE* and glpF-mediated amplification in an industrial strain brought about only about 1.2-fold increase in the volumetric CA titers. An extra copy of cas2 integration with PermE* into the S. clavuligerus DEPA genome led to 3.8-fold higher volumetric CA production by GV61. Conclusively, multicopy expression of ccaR under PglpF can result in significantly improved industrial high-titer CA producers.","PeriodicalId":23358,"journal":{"name":"Turkish Journal of Biology","volume":"41 1","pages":"342-353"},"PeriodicalIF":2.2,"publicationDate":"2017-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3906/BIY-1608-17","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48300936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Variation in drought resistance and its relationship with adaptive and physiological traits in forest trees are important in choosing suitable seed sources for reforestation and afforestation programs. A common garden experiment using 240 half-sib families originating from coastal and inland populations of Turkish red pine (Pinus brutia) in Turkey was set up with three replicates. The aims were to determine variation of drought damage, height growth, and phenology among populations and to investigate the relationship between drought damage and physiological traits (i.e. plant moisture stress and proline content). Three-year-old seedlings were subjected to drought treatment during the summer of 2000 and adaptive and physiological traits were measured. Except for bud burst, the majority of the variation resided between populations, leading to low heritability estimates for all traits. On average, inland populations were more resistant to drought and taller, with earlier bud burst and bud set times, than coastal populations. Proline content increased with higher drought damage, especially in cold-resistant and inland families. Inland populations are more drought-resistant than coastal populations. The results of the study demonstrate the possibility of selection for drought resistance for Turkish red pine at the population level.
{"title":"Population variation in drought resistance and its relationship with adaptive and physiological seedling traits in Turkish red pine (Pinus brutia Ten.)","authors":"G. Kandemir, S. Önde, F. Temel, Z. Kaya","doi":"10.3906/BIY-1608-77","DOIUrl":"https://doi.org/10.3906/BIY-1608-77","url":null,"abstract":"Variation in drought resistance and its relationship with adaptive and physiological traits in forest trees are important in choosing suitable seed sources for reforestation and afforestation programs. A common garden experiment using 240 half-sib families originating from coastal and inland populations of Turkish red pine (Pinus brutia) in Turkey was set up with three replicates. The aims were to determine variation of drought damage, height growth, and phenology among populations and to investigate the relationship between drought damage and physiological traits (i.e. plant moisture stress and proline content). Three-year-old seedlings were subjected to drought treatment during the summer of 2000 and adaptive and physiological traits were measured. Except for bud burst, the majority of the variation resided between populations, leading to low heritability estimates for all traits. On average, inland populations were more resistant to drought and taller, with earlier bud burst and bud set times, than coastal populations. Proline content increased with higher drought damage, especially in cold-resistant and inland families. Inland populations are more drought-resistant than coastal populations. The results of the study demonstrate the possibility of selection for drought resistance for Turkish red pine at the population level.","PeriodicalId":23358,"journal":{"name":"Turkish Journal of Biology","volume":"41 1","pages":"256-267"},"PeriodicalIF":2.2,"publicationDate":"2017-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3906/BIY-1608-77","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47464758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hande Yeğenoğlu, B. Aslim, Burcu Guven, A. Zengin, I. Boyaci, Z. Suludere, U. Tamer
Magnetic gold nanoparticles are used in various biomedical, biochemistry, and biotechnology applications due to their controllable size distribution, long-term stability, reduced toxicity, and biocompatibility. Different coating materials, such as proteins, carbohydrates, lipids, and polyphenols, are applied to enhance the biocompatibility of nanoparticles. In this study, the effects of surface coatings of core-shell structured Fe@Au nanosphere magnetic nanoparticles with regard to antioxidant capacity and cytotoxic, anticarcinogenic, and genotoxic properties were investigated. The obtained results demonstrated that avidin-coated Fe@Au nanospheres had higher antioxidant capacities than uncoated nanospheres. Neither avidin-coated nor uncoated nanoparticles had a cytotoxic effect on normal cells (human gingival fibroblast cell line, HGF-1). In addition, they had anticarcinogenic effects on human cervical carcinoma (HeLa), human breast adenocarcinoma (MCF-7), and human colorectal adenocarcinoma (CCL-221). The genotoxic effects of nanoparticles were also evaluated with DNA tail damage ratio.
{"title":"The comparison of antioxidant capacity and cytotoxic, anticarcinogenic, and genotoxic effects of Fe@Au nanosphere magnetic nanoparticles","authors":"Hande Yeğenoğlu, B. Aslim, Burcu Guven, A. Zengin, I. Boyaci, Z. Suludere, U. Tamer","doi":"10.3906/biy-1607-2","DOIUrl":"https://doi.org/10.3906/biy-1607-2","url":null,"abstract":"Magnetic gold nanoparticles are used in various biomedical, biochemistry, and biotechnology applications due to their controllable size distribution, long-term stability, reduced toxicity, and biocompatibility. Different coating materials, such as proteins, carbohydrates, lipids, and polyphenols, are applied to enhance the biocompatibility of nanoparticles. In this study, the effects of surface coatings of core-shell structured Fe@Au nanosphere magnetic nanoparticles with regard to antioxidant capacity and cytotoxic, anticarcinogenic, and genotoxic properties were investigated. The obtained results demonstrated that avidin-coated Fe@Au nanospheres had higher antioxidant capacities than uncoated nanospheres. Neither avidin-coated nor uncoated nanoparticles had a cytotoxic effect on normal cells (human gingival fibroblast cell line, HGF-1). In addition, they had anticarcinogenic effects on human cervical carcinoma (HeLa), human breast adenocarcinoma (MCF-7), and human colorectal adenocarcinoma (CCL-221). The genotoxic effects of nanoparticles were also evaluated with DNA tail damage ratio.","PeriodicalId":23358,"journal":{"name":"Turkish Journal of Biology","volume":"41 1","pages":"302-313"},"PeriodicalIF":2.2,"publicationDate":"2017-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41663630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The dopamine transporter (DAT) plays a role in the termination of dopaminergic neurotransmission; thereby it is accepted as the primary target of various psychostimulants. N-terminal phosphorylation of DAT has been proposed as a regulator in different DAT functions, such as amphetamine-induced efflux or PKC/PKA-mediated responses. To understand the role of N-terminal conformational changes in dopamine transporter structure and function, the fluorescence resonance energy transfer (FRET) method was applied to various DAT constructs fluorescently labeled at the N-terminus and substrate-induced conformational changes were determined using rhodamine-labeled cocaine analog JHC1-64 in three DAT mutants. The results indicated that the construct with YFP inserted into the N-terminal position-55 displayed effective interaction with the substrate and simultaneous mutation of two serine residues (S7 and S12) to alanine or aspartic acid demonstrated similar phenotypes as their wild-type (WT) counterparts. FRET was detectable for N-terminal p55 YFP WT, Ser/Ala, and Ser/Asp forms, but there was no significant difference among the three mutants, contrary to our expectations based on the previously proposed roles of these serine residues. In addition, three mutants (K264A, D345A, and Y335A) implemented in the position-55 YFP background were also investigated and the importance of Y335 in the translocation cycle and in the process of substrate release was verified.
{"title":"FRET-based characterization of K264A, D345A, and Y335Amutants in the human dopamine transporter","authors":"O. Orun, P. Tiber","doi":"10.3906/BIY-1607-9","DOIUrl":"https://doi.org/10.3906/BIY-1607-9","url":null,"abstract":"The dopamine transporter (DAT) plays a role in the termination of dopaminergic neurotransmission; thereby it is accepted as the primary target of various psychostimulants. N-terminal phosphorylation of DAT has been proposed as a regulator in different DAT functions, such as amphetamine-induced efflux or PKC/PKA-mediated responses. To understand the role of N-terminal conformational changes in dopamine transporter structure and function, the fluorescence resonance energy transfer (FRET) method was applied to various DAT constructs fluorescently labeled at the N-terminus and substrate-induced conformational changes were determined using rhodamine-labeled cocaine analog JHC1-64 in three DAT mutants. The results indicated that the construct with YFP inserted into the N-terminal position-55 displayed effective interaction with the substrate and simultaneous mutation of two serine residues (S7 and S12) to alanine or aspartic acid demonstrated similar phenotypes as their wild-type (WT) counterparts. FRET was detectable for N-terminal p55 YFP WT, Ser/Ala, and Ser/Asp forms, but there was no significant difference among the three mutants, contrary to our expectations based on the previously proposed roles of these serine residues. In addition, three mutants (K264A, D345A, and Y335A) implemented in the position-55 YFP background were also investigated and the importance of Y335 in the translocation cycle and in the process of substrate release was verified.","PeriodicalId":23358,"journal":{"name":"Turkish Journal of Biology","volume":"41 1","pages":"378-387"},"PeriodicalIF":2.2,"publicationDate":"2017-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3906/BIY-1607-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48261635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Edyta Wojtaś, A. Zachwieja, Anna Zwyrzykowska-Wodzińska, R. Kupczyński, K. Marycz
* Correspondence: edyta.wojtas@up.wroc.pl
*通信：edyta.wojtas@up.wroc.pl
{"title":"The application of mesenchymal progenitor stem cells for the reduction of oxidative stress in animals","authors":"Edyta Wojtaś, A. Zachwieja, Anna Zwyrzykowska-Wodzińska, R. Kupczyński, K. Marycz","doi":"10.3906/BIY-1603-13","DOIUrl":"https://doi.org/10.3906/BIY-1603-13","url":null,"abstract":"* Correspondence: edyta.wojtas@up.wroc.pl","PeriodicalId":23358,"journal":{"name":"Turkish Journal of Biology","volume":"41 1","pages":"12-19"},"PeriodicalIF":2.2,"publicationDate":"2017-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3906/BIY-1603-13","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47733788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Q. Lin, Peng Zhu, R. Carballar-Lejarazú, I. Gelbič, X. Guan, Lei Xu, Lingling Zhang
Qiuqiu LIN, Pengli ZHU, Rebeca CARBALLAR-LEJARAZÚ, Ivan GELBIČ*, Xiong GUAN, Lei XU, Lingling ZHANG* Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, Fujian Agriculture and Forestry University, Fuzhou, Fujian, P. R. China Fujian-Taiwan Joint Center for Ecological Control of Crop Pests, Fujian Agriculture and Forestry University, Fuzhou, P. R. China Department of Molecular Biology and Biochemistry, University of California Irvine, Irvine, CA, USA Biology Centre of the Czech Academy of Sciences, Institute of Entomology, České Budějovice, Czech Republic
{"title":"The colonization of Bacilllus thuringiensis strains in bryophytes","authors":"Q. Lin, Peng Zhu, R. Carballar-Lejarazú, I. Gelbič, X. Guan, Lei Xu, Lingling Zhang","doi":"10.3906/BIY-1510-16","DOIUrl":"https://doi.org/10.3906/BIY-1510-16","url":null,"abstract":"Qiuqiu LIN, Pengli ZHU, Rebeca CARBALLAR-LEJARAZÚ, Ivan GELBIČ*, Xiong GUAN, Lei XU, Lingling ZHANG* Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, Fujian Agriculture and Forestry University, Fuzhou, Fujian, P. R. China Fujian-Taiwan Joint Center for Ecological Control of Crop Pests, Fujian Agriculture and Forestry University, Fuzhou, P. R. China Department of Molecular Biology and Biochemistry, University of California Irvine, Irvine, CA, USA Biology Centre of the Czech Academy of Sciences, Institute of Entomology, České Budějovice, Czech Republic","PeriodicalId":23358,"journal":{"name":"Turkish Journal of Biology","volume":"41 1","pages":"41-48"},"PeriodicalIF":2.2,"publicationDate":"2017-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3906/BIY-1510-16","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48923579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Bocian, K. Hus, M. Jaromin, M. Tyrka, A. Lyskowski
* Correspondence: bocian@prz.edu.pl
*通信：bocian@prz.edu.pl
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