Veterinary journal最新文献_第8页

Evaluation of serum FGF23 and serum magnesium levels in dogs and cats with acute kidney injury 急性肾损伤犬和猫血清FGF23和血清镁水平的评价。

IF 3.1 2区农林科学 Q1 VETERINARY SCIENCES

Veterinary journal

Pub Date : 2025-12-01 DOI: 10.1016/j.tvjl.2025.106495

Akiko Miyagawa, Mio Ishizaka, Hirosumi Miyakawa, Yuichi Miyagawa, Naoyuki Takemura

Acute kidney injury (AKI) is caused by sudden kidney damage. In studies using mouse model of AKI, the blood concentrations of fibroblast growth factor-23 (FGF-23) increased persistently in AKI. In addition, higher FGF-23 concentrations were associated with the progression of AKI to chronic kidney disease (CKD), and AKI disrupted magnesium homeostasis. However, no study has evaluated these factors in dogs and cats with AKI. This study aimed to measure serum FGF-23 and magnesium concentrations in dogs and cats with AKI. This cross-sectional study used data available in medical records. The AKI group included six dogs and six cats, while the control group consisted of eight dogs and six cats, all of which were client-owned animals. Results showed that in dogs, the AKI group had significantly higher serum FGF-23 (median control, 278.5 pg/mL; AKI, 2104.0 pg/mL; P = 0.02), and magnesium (median control, 2.0 mg/dL; AKI, 2.65 mg/dL; P = 0.043) concentrations than the control group. In cats, the AKI group had significantly higher FGF-23 (median control, 138 pg/mL; AKI, 1195 pg/mL; P = 0.015) concentrations than the control group. In addition, serum FGF-23 concentrations were a significant positive correlation with serum creatinine (r = 0.67, P = 0.018) and phosphate concentrations (r = 0.893, P < 0.001) in dogs.To the best of knowledge, the current study first reported that dogs and cats with AKI had increased serum FGF-23 concentrations. Moreover, dogs with AKI exhibited high serum magnesium concentrations.

急性肾损伤（AKI）是由突发性肾损害引起的。在AKI小鼠模型研究中，成纤维细胞生长因子-23 （FGF-23）血药浓度持续升高。此外，较高的FGF-23浓度与AKI向慢性肾脏疾病（CKD）的进展有关，AKI破坏了镁的体内平衡。然而，没有研究评估这些因素对患有AKI的狗和猫的影响。本研究旨在测量AKI狗和猫的血清FGF-23和镁浓度。这项横断面研究使用了医疗记录中的数据。AKI组包括6只狗和6只猫，而对照组包括8只狗和6只猫，它们都是客户拥有的动物。结果：AKI组狗血清FGF-23（对照组中值为278.5pg/mL； AKI组为2104.0pg/mL; P = 0.02）和镁（对照组中值为2.0mg/dL； AKI组为2.65mg/dL; P = 0.043）浓度显著高于对照组。在猫中，AKI组的FGF-23浓度显著高于对照组（对照组中位数为138pg/mL； AKI组为1195pg/mL； P = 0.015）。此外，血清FGF-23浓度与血清肌酐（r = 0.67, P = 0.018）和磷酸盐浓度（r = 0.893, P < 0.001）呈显著正相关。据我们所知，目前的研究首次报道了患有AKI的狗和猫的血清FGF-23浓度升高。此外，AKI犬表现出较高的血清镁浓度。

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引用次数: 0

Immune, stress, and redox status biomarkers in newborn calves: Dynamics in serum and saliva during the first week of life 新生牛犊的免疫、应激和氧化还原状态生物标志物：生命第一周血清和唾液的动态

IF 3.1 2区农林科学 Q1 VETERINARY SCIENCES

Veterinary journal

Pub Date : 2025-11-30 DOI: 10.1016/j.tvjl.2025.106524

F.G. Silva , J.J. Cerón , C. Conceição , L. Pardo-Marín , A. Muñoz , J.O.L. Cerqueira , S.R. Silva , E. Lamy

This study investigated serum and saliva immunity, stress, and redox status biomarkers in dairy calves during the first week of life. Blood and saliva samples were collected from 20 calves on days 0, 1, 2, and 7 for the analysis of biomarkers related to innate immunity (myeloperoxidase, calprotectin), cellular (adenosine deaminase), humoral immunity (gamma-glutamyl transferase), stress (α-amylase) and redox status (ferric reducing ability). Colostrum was given to all calves (3.8 ± 0.64 L) within 96 ± 73 min of birth. On day 7, some calves showed signs of neonatal calf diarrhea, most likely due to nutritional factors. All biomarkers were measurable in both fluids from day 0. Myeloperoxidase, Calprotectin and adenosine deaminase were higher in saliva than in serum and significantly increased after colostrum intake. Biomarkers of humoral immunity were higher in serum, increasing with colostrum intake. A positive correlation was found between gamma-glutamyl transferase and IgG and IgA in saliva. Biomarkers of stress and redox status did not appear to change with colostrum intake. Neonatal calf diarrhea led to increased concentrations of some of the biomarkers analyzed, such as myeloperoxidase and α-amylase. These results highlight the effect of colostrum on the cellular and humoral immunity of calves and provide insights into the potential use of saliva to monitor immune and stress status.

本研究调查了奶牛出生后第一周的血清和唾液免疫、应激和氧化还原状态生物标志物。在第0、1、2和7天采集20头犊牛的血液和唾液样本，分析与先天免疫（髓过氧化物酶、钙保护蛋白）、细胞（腺苷脱氨酶）、体液免疫（γ -谷氨酰转移酶）、应激（α-淀粉酶）和氧化还原状态（铁还原能力）相关的生物标志物。所有犊牛在出生后96±73min内饲喂初乳（3.8±0.64L）。在第7天，一些小牛表现出新生儿牛犊腹泻的迹象，很可能是由于营养因素。从第0天开始，两种液体中的所有生物标志物均可测量。唾液中髓过氧化物酶、钙保护蛋白和腺苷脱氨酶高于血清，初乳摄入后显著升高。体液免疫的生物标志物在血清中较高，随着初乳摄入量的增加而增加。唾液中γ -谷氨酰转移酶与IgG、IgA呈显著正相关。应激和氧化还原状态的生物标志物似乎没有随着初乳的摄入而改变。新生儿小牛腹泻导致一些生物标志物的浓度升高，如髓过氧化物酶和α-淀粉酶。这些结果强调了初乳对小牛细胞和体液免疫的影响，并为唾液监测免疫和应激状态的潜在用途提供了见解。

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引用次数: 0

Discovery proteomics to detect salivary biomarkers in dog and human periodontitis: Mass spectrometry-based analysis 发现蛋白质组学检测狗和人类牙周炎的唾液生物标志物：基于质谱的分析。

IF 3.1 2区农林科学 Q1 VETERINARY SCIENCES

Veterinary journal

Pub Date : 2025-11-29 DOI: 10.1016/j.tvjl.2025.106521

Paras Ahmad , Candace Lowe , Paulos Chumala , Walter L. Siqueira

Periodontitis, prevalent in humans and dogs, is a chronic inflammatory disease affecting the oral hard and soft tissues. Salivary proteomics and protein homology may offer a non-invasive diagnosis, benefiting both species due to similarities in disease etiology and progression. This study compared (i) the proteomics profile of healthy and periodontitis dogs using mass spectrometry; and (ii) the protein homology of the identified differentially expressed proteins between humans and dogs. Twenty dogs were examined over six months, divided into two groups: ten healthy (control) and ten with periodontitis. Saliva samples were collected and analyzed using mass spectrometry. The study identified 855 proteins in healthy dogs and 849 in dogs with periodontitis, with a 96 % overlap. Notable shared proteins included abnormal spindle-like microcephaly-associated protein homolog, DNA-dependent protein kinase catalytic subunit, and myosin-2, while specific proteins, including myosin-13 and fibrocystin, were unique to healthy dogs, and G2/mitotic-specific cyclin-B3 and hemoglobin subunit beta were unique to periodontitis dogs. In periodontitis dogs, proteins adenosine receptor A2b, apoptotic protease-activating factor 1, and calcitonin gene-related peptide 1 were upregulated, and cytotoxic T-lymphocyte protein 4, laforin, and lens fiber major intrinsic protein were downregulated. The proteins were involved in second messenger signaling and drug response pathways, with complex interaction networks identified. Homology between human and dog proteins ranged from 78.3 % to 100 %, suggesting that these shared proteins could be relevant for cross-species periodontal research and diagnostics.

牙周炎是一种影响口腔硬组织和软组织的慢性炎症性疾病，常见于人类和狗。唾液蛋白质组学和蛋白质同源性可以提供非侵入性诊断，由于疾病病因和进展的相似性，这两个物种都受益。本研究使用质谱法比较了健康犬和牙周炎犬的蛋白质组学特征；（ii）鉴定的人与狗之间差异表达蛋白的蛋白同源性。20只狗在6个月的时间里接受了检查，分为两组：10只健康（对照组）和10只患有牙周炎。唾液样本采集并使用质谱分析。该研究在健康狗身上发现了855种蛋白质，在牙周炎狗身上发现了849种蛋白质，有96%的重叠。值得注意的共同蛋白包括异常纺锤体样小头畸形相关蛋白同源物、dna依赖性蛋白激酶催化亚基和肌球蛋白-2，而特异性蛋白包括肌球蛋白-13和纤维囊蛋白是健康犬所特有的，G2/有丝分裂特异性细胞周期蛋白- b3和血红蛋白亚基β是牙周炎犬所特有的。在牙周炎犬中，腺苷受体A2b蛋白、凋亡蛋白酶激活因子1、降钙素基因相关肽1上调，细胞毒性t淋巴细胞蛋白4、去甲素、晶状体纤维主要内在蛋白下调。这些蛋白参与第二信使信号和药物反应途径，具有复杂的相互作用网络。人和狗蛋白的同源性在78.3%至100%之间，表明这些共享蛋白可能与跨物种牙周研究和诊断有关。

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引用次数: 0

Trained dogs can detect the odor of hemangiosarcoma in canine blood samples 经过训练的狗可以在狗的血液样本中发现血管肉瘤的气味。

IF 3.1 2区农林科学 Q1 VETERINARY SCIENCES

Veterinary journal

Pub Date : 2025-11-29 DOI: 10.1016/j.tvjl.2025.106522

Clara Wilson , Samantha Holden , Julianna King , Amritha Mallikarjun , Molly Buis , Cynthia M. Otto

Hemangiosarcoma (HSA) is a common, aggressive, and deadly vascular cancer in dogs that is usually diagnosed only at advanced stages. Because treatment options are limited once HSA is advanced, early detection is essential to improving survival and quality of life. Five trained bio-detection dogs were evaluated using double-blinded tests with automated olfactometer line-ups containing blood serum samples from dogs with confirmed HSA, non-cancerous diseases other than HSA (diseased controls), and healthy controls. All test samples were novel to the dogs. Across all 423 blinded trials, accuracy was 70.0 % (range = 57.1–78.6 %). First-trial accuracy, representing each dog’s initial response to a novel matched sample set, averaged 70.0 % (range = 58.3–83.3 %). When considering each dog’s first encounter with each sample, dogs achieved an overall sensitivity of 70.0 % and specificity of 70.0 %. A mixed-effects logistic regression showed that dogs alerted to HSA samples in 73.4 % of presentations, compared with 21.3 % of diseased controls and 17.1 % of healthy controls. Dogs were over 10 times more likely to alert to HSA than to diseased controls (OR = 10.2, p < .001) and over 13 times more likely than to healthy controls (OR = 13.3, p < .001). This study finds that trained dogs can distinguish serum samples from dogs with HSA from those of healthy and diseased controls, indicating that HSA produces a detectable odor signature. Conclusions are constrained by the limited number of HSA samples. These results suggest a potential feasibility of VOC-based detection for canine HSA.

血管肉瘤（HSA）是一种常见的、侵袭性的、致命的血管癌，通常只有在晚期才被诊断出来。由于一旦HSA进展，治疗选择有限，因此早期发现对于改善生存和生活质量至关重要。5只经过训练的生物检测犬采用双盲试验，采用自动嗅觉仪对血清样本进行评估，这些血清样本分别来自确诊HSA、非HSA的非癌性疾病（患病对照组）和健康对照组。所有的测试样本对狗来说都是新的。在所有423项盲法试验中，准确率为70.0%（范围= 57.1%至78.6%）。首试准确率，代表每只狗对新的匹配样本集的初始反应，平均为70.0%（范围= 58.3%至83.3%）。当考虑到每只狗第一次遇到每个样本时，狗的总体灵敏度为70.0%，特异性为70.0%。混合效应逻辑回归显示，73.4%的狗对HSA样本有反应，而患病对照组为21.3%，健康对照组为17.1%。狗对HSA的警觉性是患病对照的10倍以上(OR = 10.2, p

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引用次数: 0

Immunomodulatory and anti-proliferative effects of canine adipose-derived stromal vascular fraction on peripheral blood mononuclear cells: An in vitro model for therapeutic applications 犬脂肪源性基质血管组分对外周血单核细胞的免疫调节和抗增殖作用：用于治疗应用的体外模型

IF 3.1 2区农林科学 Q1 VETERINARY SCIENCES

Veterinary journal

Pub Date : 2025-11-28 DOI: 10.1016/j.tvjl.2025.106520

Hitesh Rana, Ratan Kumar Choudhary

This study investigated the immunomodulatory and anti-proliferative effects of canine adipose-derived stromal vascular fraction (SVF) on peripheral blood mononuclear cells (PBMCs) from healthy and lymphoma-affected dogs. SVF, a rich source of mesenchymal stem cells (MSCs), was isolated from canine periovarian adipose tissue and characterized by flow cytometry (CD45, CD44, and CD90), immunocytochemistry, and gene expression analyses (CD44, CD90, and CD105). Coculturing SVF with PBMCs showed that SVF significantly increased the population doubling time and decreased Ki-67 expression in PBMCs (3.5-fold) in a dose-dependent manner. Furthermore, SVF exhibited immunomodulatory properties by remarkably down-regulating the pro-inflammatory cytokine TNF-α (44-fold; p = 0.0003) and up-regulating the anti-inflammatory cytokine PTGS1 (88-fold; p = 0.008). These findings confirm that canine SVF contains functional MSCs and possesses significant immunomodulatory and anti-proliferative capabilities against PBMCs, suggesting its therapeutic potential. This study also demonstrates coculture of SVF and PBMC as an in vitro model for studying therapeutic applications of stem cells in canine inflammatory and proliferative conditions.

本研究探讨了犬脂肪源性基质血管组分（SVF）对健康犬和淋巴瘤犬外周血单个核细胞（PBMCs）的免疫调节和抗增殖作用。SVF是一种丰富的间充质干细胞（MSCs）来源，从犬骨膜脂肪组织中分离出来，并通过流式细胞术（CD45、CD44和CD90）、免疫细胞化学和基因表达分析（CD44、CD90和CD105）对其进行了表征。SVF与PBMCs共培养表明，SVF显著增加了PBMCs的群体倍增时间，降低了Ki-67的表达（3.5倍），并呈剂量依赖性。此外，SVF通过显著下调促炎细胞因子TNF-α（44倍，p = 0.0003）和上调抗炎细胞因子PTGS1（88倍，p = 0.008）表现出免疫调节特性。这些研究结果证实，犬SVF含有功能性MSCs，对PBMCs具有显著的免疫调节和抗增殖能力，提示其治疗潜力。本研究还证明了SVF和PBMC的共培养作为研究干细胞在犬炎症和增殖条件下的治疗应用的体外模型。

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引用次数: 0

Effects of classical music and supervisory authority on anxiety and surgical performance in veterinary students during first ovariohysterectomy 古典音乐和监督权威对兽医学生第一次卵巢子宫切除术中焦虑和手术表现的影响。

IF 3.1 2区农林科学 Q1 VETERINARY SCIENCES

Veterinary journal

Pub Date : 2025-11-28 DOI: 10.1016/j.tvjl.2025.106518

Atakan Cortu , Muhammed Albayrak , Halil Ozan Demirtas , Latif Emrah Yanmaz

Anxiety during the first live-animal surgery represents a major challenge in veterinary training, potentially affecting both performance and learning outcomes. Classical music has been suggested as a non-invasive strategy to moderate stress, while the degree of supervisory authority may further modulate students’ emotional and behavioural responses. This study evaluated the effects of classical music exposure and supervisory authority on perioperative anxiety and surgical performance in final-year veterinary students performing their first feline ovariohysterectomy. Forty students were randomly allocated into four groups (n = 10 each): assistant professor supervision without music (AP), assistant professor with music (AP-M), research assistant without music (RA), and research assistant with music (RA-M). Music consisted of Mozart’s Sonata for Two Pianos in D Major, K.448, delivered via headphones for 30 min preoperatively. Anxiety was measured using the State-Trait Anxiety Inventory before and after intervention and following surgery. Operative duration was recorded from skin incision to closure. The AP-M was associated with increased state anxiety, whereas no significant changes occurred in other groups. Operative times were longest in AP-M and RA, and shortest in RA-M. Trait anxiety correlated positively with operative duration, while state anxiety was not directly linked to performance. In conclusion, music may heighten anxiety under high-authority supervision but improve efficiency in less authoritative contexts. Educational strategies should prioritise supportive supervision and consider tailoring auditory interventions to optimise student wellbeing and performance.

第一次活体动物手术期间的焦虑是兽医培训的主要挑战，可能会影响表现和学习结果。古典音乐被认为是一种非侵入性的缓解压力的策略，而监管权威的程度可能会进一步调节学生的情绪和行为反应。本研究评估了古典音乐的暴露和监督权力对兽医学生围手术期焦虑和手术表现的影响，这些学生是第一次进行猫卵巢子宫切除术。40名学生被随机分为4组（n = 10）：助理教授指导无音乐（AP），助理教授有音乐（AP- m），研究助理无音乐（RA），研究助理有音乐（RA- m）。音乐由莫扎特D大调双钢琴奏鸣曲K.448组成，术前通过耳机播放30分钟。在干预前后和手术后使用状态-特质焦虑量表测量焦虑。记录从皮肤切口到闭合的手术时间。AP-M与状态焦虑增加有关，而其他组无明显变化。AP-M和RA组手术时间最长，RA- m组最短。特质焦虑与手术持续时间呈正相关，而状态焦虑与手术表现无直接关系。综上所述，音乐可能会增加高权威监督下的焦虑，但在不那么权威的环境下会提高效率。教育策略应优先考虑支持性监督，并考虑定制听觉干预，以优化学生的福祉和表现。

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引用次数: 0

Hemoperfusion for veterinary toxicities: International renal interest society best practices consensus guidelines 血液灌流治疗兽医毒性：国际肾脏利益协会最佳实践共识指南。

IF 3.1 2区农林科学 Q1 VETERINARY SCIENCES

Veterinary journal

Pub Date : 2025-11-28 DOI: 10.1016/j.tvjl.2025.106519

Emmanuelle M. Butty , Jeff Barnes , Erica Reineke , Cedric Dufayet , Jiwoong Her , Larry D. Cowgill

New adsorptive devices for hemoperfusion are available in veterinary medicine for the management of toxin exposures and life-threatening toxin decontamination. This document is offered as the currently recommended standard-of-care and best practice guidelines derived from the clinical experiences of experts treating toxicities with hemoperfusion. Much of the evidence available to support recommendations on extracorporeal management of veterinary intoxications is limited, inconsistent, indirect, or of poor quality. Consequently, a formal consensus methodology which relies heavily on expert opinion was used to validate the proposed guidelines of the International Renal Interest Society’s (IRIS) Hemoperfusion Study Group. An independently selected voting panel of noted authorities in veterinary extracorporeal medicine were charged to vote and comment on each Study Group recommendation in isolation and without supplemental rationale. To achieve consensus, a minimum of 75 % of the voting participants chose “strongly agree” or “agree” compared to neutral or disagree responses. As this is a rapidly evolving therapeutic modality, and the technologies are changing with similar pace, we expect these clinical guidelines will also evolve rapidly. It will be important to review updates from the manufacturers of hemoperfusion devices to obtain the most current treatment recommendations for specific adsorptive devices.

新的吸附装置血液灌流可用于兽医毒素暴露管理和危及生命的毒素净化。本文件是目前推荐的标准护理和最佳实践指南，源自专家治疗血液灌流毒性的临床经验。可用于支持兽医中毒体外管理建议的许多证据是有限的、不一致的、间接的或质量差的。因此，一个正式的共识方法，严重依赖于专家意见，被用来验证国际肾脏利益协会（IRIS）血液灌流研究小组提出的指南。由兽医体外医学知名机构组成的独立选出的投票小组负责对研究小组的每项建议进行单独投票和评论，且不提供补充理由。为了达成共识，至少75%的投票参与者选择了“非常同意”或“同意”，而不是中立或不同意的回答。由于这是一种快速发展的治疗方式，技术也在以类似的速度变化，我们预计这些临床指南也将迅速发展。回顾血液灌流装置制造商的最新情况，以获得针对特定吸附装置的最新治疗建议，这一点很重要。

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引用次数: 0

Epidemiology and clinical significance of Mycoplasma bovis infection in calf feedlots in Spain 西班牙犊牛饲养场牛支原体感染流行病学及临床意义。

IF 3.1 2区农林科学 Q1 VETERINARY SCIENCES

Veterinary journal

Pub Date : 2025-11-27 DOI: 10.1016/j.tvjl.2025.106517

Christian de la Fe , Xóchitl Hernández , Juan Alcázar , Antonio Sánchez , David del Olmo , Antonio Fernández , Héctor Ramírez , Ginés Luján , Joaquín Amores-Iniesta , Juan Carlos Corrales

The presence, dynamics and persistence of Mycoplasma (M.) bovis in 12 batches of grazing calves during the first month of stay at the feedlots was analyzed. Nasopharyngeal swabs were weekly collected between days 0 and 28 to detect the presence of M. bovis by culture and PCR. The animals were daily monitored for the presence of respiratory disease. The results showed the presence of different epidemiological situations at arrival, detecting the presence of uninfected or with a low prevalence groups or batches with a variable prevalence (13.3–86.7 %). Subsequently, at least one calf scored positive in all the batches 14 days after arrival and almost all calves (n = 166) resulted infected after 28 days. Calves infected with M. bovis tested positive in 3 of the 5 samples taken (mean 3.06). The presence of respiratory signs was statistically significant in infected vs. uninfected ones, with symptoms appearing earlier in batches where the initial prevalence of M. bovis was higher. The molecular characterization of the isolates confirmed the presence of more than one biotype circulating in the same batch or even calf, which could be associated to the presence of more severe clinical findings. Most of the isolates were classified as biotype st-3 but three biotypes were detected, resulting all of them multiresistant to macrolides, tetracyclines and lincomycin with a variable sensitivity to quinolones. Overall, the frequency of M. bovis infection highlights its significant epidemiological role in bovine respiratory diseases in calf fattening.

分析了12批放牧犊牛在饲养场停留第一个月期间牛支原体的存在、动态和持久性。在第0天至第28天之间每周收集鼻咽拭子，通过培养和PCR检测牛分枝杆菌的存在。每天监测这些动物是否有呼吸道疾病。结果表明，入境时存在不同的流行病学情况，发现存在未感染或低流行群体或批次，流行率变化（13.3-86.7%）。随后，在所有批次中，至少有一头小牛在抵达14天后呈阳性，几乎所有的小牛（n=166）在28天后感染。在采集的5个样本中，有3个样本检测出感染牛分枝杆菌的小牛呈阳性（平均3.06）。在感染与未感染人群中，呼吸道体征的存在具有统计学意义，在最初牛支原体流行率较高的批次中，症状出现得更早。分离物的分子特征证实，在同一批次甚至小牛中存在不止一种生物型，这可能与存在更严重的临床表现有关。多数分离株为st-3型，但检测到3种生物型，均对大环内酯类、四环素类和林可霉素多重耐药，对喹诺酮类药物敏感性不同。总体而言，牛支原体感染的频率突出了其在犊牛育肥过程中牛呼吸道疾病中的重要流行病学作用。

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引用次数: 0

The impact of medical ozone on renal ischemic/reperfusion injury: A prospective randomized controlled study in dogs 医用臭氧对狗肾缺血/再灌注损伤的影响：一项前瞻性随机对照研究。

IF 3.1 2区农林科学 Q1 VETERINARY SCIENCES

Veterinary journal

Pub Date : 2025-11-27 DOI: 10.1016/j.tvjl.2025.106509

Haytham Aamer , Eman M Nour , Ayman Refaie , Mohamed Youssef , Eiad A Ramzy , Sherry Khater , Maged El-Ashker

This study evaluated the protective effects of ozone therapy in dogs with experimentally induced renal ischemia–reperfusion injury (IRI). Fifteen clinically healthy male mongrel dogs were randomly allocated into five groups. Group 1 served as the sham-operated control. Bilateral renal ischemia was induced in Group 2 (IRI control) and in three additional treatment groups receiving ozone therapy once daily via rectal insufflation. Group 3 received ozone preconditioning before IRI (PRE/IRI), Group 4 received ozone postconditioning after IRI (POST/IRI), and Group 5 received both interventions (PRE/IRI/POST). Venous blood samples were collected at baseline (D0) and at D1, D3, W1, W2, and W4 to measure serum malondialdehyde (MDA), glutathione peroxidase (GSH-px), blood urea nitrogen (BUN), and creatinine. Ultrasound-guided renal biopsies were obtained at D0, D3, W1, W2, and W4 for histopathology and immunohistochemistry. Serum creatinine and BUN progressively increased in the IRI group but were significantly reduced in all ozone-treated groups (P = 0.002). The PRE/IRI/POST group showed the greatest improvement, with the lowest values by W4. Ozone preconditioning also modulated early oxidative stress, preventing the initial rise in MDA and partially preserving GSH-px activity. Histopathological examination revealed marked acute tubular necrosis in the IRI group, whereas combined pre- and postconditioning produced the highest tubular regeneration scores. Immunohistochemistry supported reduced renal injury in the treated groups, with lower caspase-3 expression and higher HIF-1α activation, most notably in the combined therapy group. These findings suggest that ozone therapy is a safe and potentially effective strategy to mitigate renal IRI. Combined ozone preconditioning and postconditioning provided the greatest protection by reducing oxidative stress and apoptosis while promoting tubular regeneration and may represent a promising adjunctive approach in clinical settings such as kidney transplantation.

本研究评价了臭氧治疗对实验性肾缺血再灌注损伤（IRI）犬的保护作用。临床健康雄性杂种犬15只，随机分为5组。第一组为假手术对照组。2组（IRI）和3个治疗组（每天1次）通过直肠注入臭氧治疗)诱导双侧肾缺血。第3组在IRI前进行臭氧预处理（PRE/IRI），第4组在IRI后进行臭氧后处理（POST/IRI），第5组同时进行预处理/IRI/POST。在基线（D0）和D1、D3、W1、W2、W4采集静脉血，测定血清丙二醛（MDA）、谷胱甘肽过氧化物酶（GSH-px）、尿素氮（BUN）和肌酐。在D0、D3、W1、W2和W4行超声引导肾活检，进行组织病理学和免疫组织化学检查。IRI组血清肌酐和BUN逐渐升高，而臭氧处理组均显著降低（P = 0.002）。PRE/IRI/POST组改善最大，在W4时达到最低值。臭氧预处理还可以调节早期氧化应激，阻止MDA的初始升高，并部分保持GSH-px活性。组织病理学检查显示IRI组有明显的急性肾小管坏死，而综合预处理和后处理的肾小管再生评分最高。免疫组化支持治疗组肾损伤减轻，caspase-3表达降低，HIF-1α活化升高，在联合治疗组最为明显。这些发现表明臭氧治疗是一种安全且潜在有效的减轻肾IRI的策略。联合臭氧预处理和后处理通过减少氧化应激和细胞凋亡提供了最大的保护，同时促进肾小管再生，可能是临床环境中有前途的辅助方法，如肾移植。

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引用次数: 0

The hidden cost of caring: Psychological burden among caregivers of dogs with cognitive dysfunction, cancer, and age-related decline 照顾的隐性成本：患有认知功能障碍、癌症和年龄相关衰退的狗的看护者的心理负担。

IF 3.1 2区农林科学 Q1 VETERINARY SCIENCES

Veterinary journal

Pub Date : 2025-11-26 DOI: 10.1016/j.tvjl.2025.106510

Darja Pavlin , Valentina Hlebec , May Doušak , Maja Zakošek Pipan

Canine cognitive dysfunction (CCD) is a neurodegenerative disorder like Alzheimer's disease in humans, but its impact on caregivers is poorly understood. This study examines the multidimensional burden of caregivers of ageing dogs, comparing caregivers of healthy ageing dogs (HAD), dogs with chronic oncological disease (COD) and dogs with CCD. In a cross-sectional survey of 516 Slovenian dog owners, seven areas of stress were investigated: time burden, work- life interference, emotional burden, social stigmatization, financial burden, perceived adequacy of care and veterinary support. Time burden was increased by unpredictable CCD symptoms (e.g. nocturnal restlessness, disorientation), especially in households with children or multiple pets (p = 0.005). Emotional distress was significantly increased in CCD caregivers due to feelings of stigmatization (p = 0.005), while COD caregivers reported no significant increase compared to HAD. Caregivers of dogs with CCD reported significantly greater stigmatization (p < 0.001) and financial burden (p < 0.001). While all groups reported similar perceived inadequacies in care, CCD caregivers expressed significantly lower confidence in performing positive care tasks (p = 0.041). Relationships with veterinarians did not differ between groups, although regression analysis identified age, education and number of pets as predictors of the dynamics between caregivers and veterinarians. This study shows that caring for dogs with CCD is significantly more stressful than caring for healthy older dogs and, in several areas, caring for dogs with chronic oncologic diseases. The results emphasize that caring for dogs with CCD is a complex, underestimated burden that is comparable to caring for dementia patients in humans and requires targeted interventions.

犬类认知功能障碍（CCD）是一种神经退行性疾病，类似于人类的阿尔茨海默病，但人们对其对护理人员的影响知之甚少。本研究考察了老龄犬照料者的多维负担，比较了健康老龄犬（HAD）、慢性肿瘤犬（COD）和CCD犬的照料者。在对516名斯洛文尼亚狗主人的横断面调查中，研究人员调查了七个方面的压力：时间负担、工作与生活的干扰、情感负担、社会污名化、经济负担、感知到的照顾和兽医支持的充足性。不可预测的CCD症状（如夜间不安、定向障碍）增加了时间负担，特别是在有儿童或多只宠物的家庭中（p=0.005）。由于污名感，CCD照顾者的情绪困扰显著增加（p=0.005），而COD照顾者与HAD相比没有显著增加。患有CCD的狗的照顾者报告了更大的耻辱（p < 0.001）和经济负担（p < 0.001）。虽然所有组都报告了类似的护理感知不足，但CCD护理人员对执行积极护理任务的信心明显较低（p = 0.041）。与兽医的关系在各组之间没有差异，尽管回归分析确定年龄，教育程度和宠物数量是护理人员和兽医之间动态的预测因素。这项研究表明，照顾患有CCD的狗狗比照顾健康的老年狗狗压力大得多，在某些领域，照顾患有慢性肿瘤疾病的狗狗压力更大。研究结果强调，照顾患有CCD的狗是一项复杂的、被低估的负担，与照顾人类痴呆症患者相当，需要有针对性的干预。

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引用次数: 0