Wellcome Open Research最新文献

We present a genome assembly from an individual Berberis vulgaris (Streptophyta; Magnoliopsida; Ranunculales; Berberidaceae). The genome sequence has a total length of 1,297.50 megabases. Most of the assembly is scaffolded into 14 chromosomal pseudomolecules. The mitochondrial and plastid genome assemblies have lengths of 786.62 kilobases and 166.26 kilobases, respectively.

We present a genome assembly from an individual male specimen of Gymnocheta viridis (tachinid fly; Arthropoda; Insecta; Diptera; Tachinidae). The genome sequence has a total length of 600.30 megabases. Most of the assembly (98.1%) is scaffolded into 6 chromosomal pseudomolecules, including the X sex chromosome. The mitochondrial genome has also been assembled and is 19.34 kilobases in length. Gene annotation of this assembly on Ensembl identified 12,716 protein-coding genes.

We present a genome assembly from a haploid Ulota crispa gametophyte (the crisped pincushion; Streptophyta; Bryopsida; Orthotrichales; Orthotrichaceae). The genome sequence spans 275.00 megabases. Most of the assembly is scaffolded into 11 chromosomal pseudomolecules. The mitochondrial and plastid genome assemblies have lengths of 104.64 kilobases and 123.54 kilobases, respectively.

We present a genome assembly from a specimen of Pyrus communis (the pear; Streptophyta; Magnoliopsida; Rosales; Rosaceae). The genome sequence has a total length of 487.30 megabases. Most of the assembly is scaffolded into 17 chromosomal pseudomolecules. The mitochondrial and plastid genome assemblies have lengths of 443.53 kilobases and 159.93 kilobases, respectively. Gene annotation of this assembly on Ensembl identified 37,713 protein-coding genes.

We present a genome assembly from an individual male Ochropleura leucogaster (Freyer, 1831) (Radford's Flame Shoulder; Arthropoda; Insecta; Lepidoptera; Noctuidae). The genome sequence has a total length of 545.70 megabases. Most of the assembly (99.93%) is scaffolded into 31 chromosomal pseudomolecules, including the Z sex chromosome. The mitochondrial genome has also been assembled and is 15.37 kilobases in length. Gene annotation of this assembly on Ensembl identified 12,155 protein-coding genes.

Background: Given the low levels of coronavirus disease 2019 (COVID-19) vaccine coverage in sub-Saharan Africa (sSA), despite high levels of natural severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) exposures, strategies for extending the breadth and longevity of naturally acquired immunity are warranted. Designing such strategies will require a good understanding of naturally acquired immunity.

Methods: We measured whole-spike immunoglobulin G (IgG) and spike-receptor binding domain (RBD) total immunoglobulins (Igs) on 585 plasma samples collected longitudinally over five successive time points within six months of COVID-19 diagnosis in 309 COVID-19 patients. We measured antibody-neutralising potency against the wild-type (Wuhan) SARS-CoV-2 pseudovirus in a subset of 51 patients over three successive time points. Binding and neutralising antibody levels and potencies were then tested for correlations with COVID-19 severities.

Results: Rates of seroconversion increased from day 0 (day of PCR testing) to day 180 (six months) (63.6% to 100 %) and (69.3 % to 97%) for anti-spike-IgG and anti-spike-RBD binding Igs, respectively. Levels of these binding antibodies peaked at day 28 (p<0.01) and were subsequently maintained for six months without significant decay (p>0.99). Similarly, antibody-neutralising potencies peaked at day 28 (p<0.01) but declined by three-fold, six months after COVID-19 diagnosis (p<0.01). Binding antibody levels were highly correlated with neutralising antibody potencies at all the time points analysed (r>0.60, p<0.01). Levels and potencies of binding and neutralising antibodies increased with disease severity.

Conclusions: Most COVID-19 patients generated SARS-CoV-2 specific binding antibodies that remained stable in the first six months of infection. However, the respective neutralising antibodies decayed three-fold by month-six of COVID-19 diagnosis suggesting that they are short-lived, consistent with what has been observed elsewhere in the world. Thus, regular vaccination boosters are required to sustain the high levels of anti-SARS-CoV-2 naturally acquired neutralising antibody potencies in our population.

We present a genome assembly from an individual male Venusia cambrica (the Welsh Wave; Arthropoda; Insecta; Lepidoptera; Geometridae). The genome sequence spans 470.40 megabases. Most of the assembly is scaffolded into 38 chromosomal pseudomolecules, including the Z sex chromosome. The mitochondrial genome has also been assembled and is 16.44 kilobases in length. Gene annotation of this assembly on Ensembl identified 17,931 protein-coding genes.

We present a genome assembly from an individual male Tolmerus cingulatus (Brown Heath Robberfly; Arthropoda; Insecta; Diptera; Asilidae). The genome sequence has a total length of 280.00 megabases. Most of the assembly (88.86%) is scaffolded into 6 chromosomal pseudomolecules, including the X and Y sex chromosomes. The mitochondrial genome has also been assembled and is 20.2 kilobases in length. Gene annotation of this assembly on Ensembl identified 12,047 protein-coding genes.

We present a genome assembly from an individual male specimen of Plusia festucae (Gold Spot; Arthropoda; Insecta; Lepidoptera; Noctuidae). The genome sequence has a total length of 422.50 megabases. Most of the assembly (99.92%) is scaffolded into 31 chromosomal pseudomolecules, including the Z sex chromosome. The mitochondrial genome has also been assembled and is 15.3 kilobases in length. Gene annotation of this assembly on Ensembl identified 19,273 protein-coding genes.