Pub Date : 2023-01-01DOI: 10.21626/vestnik/2023-2/10
L. L. Kvachakhiya, V. K. Shormanov, Elena V. Maksina
Mexidol (3-hydroxy-6-methyl-2-ethylpyridine succinate) is a drug that exhibits neuroprotective, antihypoxic, anti-ischemic, nootropic, anti-stress and geroprotective effects and can suppress or slow down oxidation reactions. Оbjective: to develop a selective and simple method for assessing the content of mexidol in tablets. Materials and methods. The objects of the study were mexidol (3-hydroxy-6-methyl-2-ethylpyridine succinate) and Mexidol tablets 0.125 g. Derivative spectrophotometry was used as an analysis method. Results. The ability of mexidol to intensively absorb electromagnetic radiation in the quartz ultraviolet region was revealed. This phenomenon is due to the fact that the analyte molecule contains a number of chromophore groups: a system of conjugated aromatic bonds of the pyridine ring, unpaired electrons at the nitrogen atom, and an enol hydroxyl conjugated with the aromatic system In the region of 292 nm, there is a linear dependence of the derivatives of the 2nd order of the specific absorption coefficient of mexidol on the amount of analyte in the photometric solution (range 2.5-50.0 μg/ml). A calibration graph has been constructed, and its equation has been calculated by the least-squares method. The correlation coefficient exceeds 0.999. The limits of detection and quantification are 1.25 and 2.5 µg/ml, respectively. The technique is characterized by the necessary reproducibility and correctness. The values of the standard deviation and relative standard deviation in the determination of the analyte using the proposed method (n=6; P=0.95) in the tablet mass are 1.16 and 0.012, respectively, in tablets of 0.125 g, 1.32 and 0.013, respectively. Conclusion. A simple and selective method has been developed for estimating the quantitative content of mexidol in 0.125 g tablets based on the calculation of second-order derivatives of spectra.
{"title":"Determination of Mexidol in tablets by the method of derivative spectrophotometry","authors":"L. L. Kvachakhiya, V. K. Shormanov, Elena V. Maksina","doi":"10.21626/vestnik/2023-2/10","DOIUrl":"https://doi.org/10.21626/vestnik/2023-2/10","url":null,"abstract":"Mexidol (3-hydroxy-6-methyl-2-ethylpyridine succinate) is a drug that exhibits neuroprotective, antihypoxic, anti-ischemic, nootropic, anti-stress and geroprotective effects and can suppress or slow down oxidation reactions. Оbjective: to develop a selective and simple method for assessing the content of mexidol in tablets. Materials and methods. The objects of the study were mexidol (3-hydroxy-6-methyl-2-ethylpyridine succinate) and Mexidol tablets 0.125 g. Derivative spectrophotometry was used as an analysis method. Results. The ability of mexidol to intensively absorb electromagnetic radiation in the quartz ultraviolet region was revealed. This phenomenon is due to the fact that the analyte molecule contains a number of chromophore groups: a system of conjugated aromatic bonds of the pyridine ring, unpaired electrons at the nitrogen atom, and an enol hydroxyl conjugated with the aromatic system In the region of 292 nm, there is a linear dependence of the derivatives of the 2nd order of the specific absorption coefficient of mexidol on the amount of analyte in the photometric solution (range 2.5-50.0 μg/ml). A calibration graph has been constructed, and its equation has been calculated by the least-squares method. The correlation coefficient exceeds 0.999. The limits of detection and quantification are 1.25 and 2.5 µg/ml, respectively. The technique is characterized by the necessary reproducibility and correctness. The values of the standard deviation and relative standard deviation in the determination of the analyte using the proposed method (n=6; P=0.95) in the tablet mass are 1.16 and 0.012, respectively, in tablets of 0.125 g, 1.32 and 0.013, respectively. Conclusion. A simple and selective method has been developed for estimating the quantitative content of mexidol in 0.125 g tablets based on the calculation of second-order derivatives of spectra.","PeriodicalId":24060,"journal":{"name":"Курский научно-практический вестник «Человек и его здоровье»","volume":"32 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72937053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.21626/vestnik/2023-2/04
S. Povetkin
Objective is to present a review of the literature data concerning the personalization of pharmacotherapy in patients with chronic heart failure with low left ventricular ejection fraction. The article discusses modern approaches to phenotyping patients with chronic heart failure with a low left ventricular ejection fraction (HFrEF). Various criteria of phenotyping are considered, both technologically complex and having the prospect of implementation into real practice in the future, and currently being implemented. For the current period, the most acceptable approach from a practical point of view remains the phenotyping of patients with HFrEF, based on clinical, hemodynamic parameters, which allows prioritizing the appointment of medicines. The article discusses the choice of optimal classes of drugs that affect the prognosis of patients with HFrEF, based on taking into account such markers as blood pressure, heart rate, presence of atrial fibrillation, presence and degree of renal dysfunction, electrolyte balance, a combination of these parameters. A phenotypic approach to the treatment of patients with HFrEF will help to ensure the personalization of pharmacotherapy for heart failure with maximum benefit for a particular patient.
{"title":"Phenotypic approach to personalization of pharmacotherapy in patients with congenetive heart failure with low left ventricular ejection fraction","authors":"S. Povetkin","doi":"10.21626/vestnik/2023-2/04","DOIUrl":"https://doi.org/10.21626/vestnik/2023-2/04","url":null,"abstract":"Objective is to present a review of the literature data concerning the personalization of pharmacotherapy in patients with chronic heart failure with low left ventricular ejection fraction. The article discusses modern approaches to phenotyping patients with chronic heart failure with a low left ventricular ejection fraction (HFrEF). Various criteria of phenotyping are considered, both technologically complex and having the prospect of implementation into real practice in the future, and currently being implemented. For the current period, the most acceptable approach from a practical point of view remains the phenotyping of patients with HFrEF, based on clinical, hemodynamic parameters, which allows prioritizing the appointment of medicines. The article discusses the choice of optimal classes of drugs that affect the prognosis of patients with HFrEF, based on taking into account such markers as blood pressure, heart rate, presence of atrial fibrillation, presence and degree of renal dysfunction, electrolyte balance, a combination of these parameters. A phenotypic approach to the treatment of patients with HFrEF will help to ensure the personalization of pharmacotherapy for heart failure with maximum benefit for a particular patient.","PeriodicalId":24060,"journal":{"name":"Курский научно-практический вестник «Человек и его здоровье»","volume":"27 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74732967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.21626/vestnik/2023-2/09
Evgeniya V. Kompantseva, Darya N. Lutsenko, E. R. Garcia, A. Ozerov, T. M. Dementieva
The original biologically active compound (BAS) N-[2-[4-oxo-3(4H)-quinazolinyl]propionyl]-guanidine (VMA-13-15), which has cardioprotective and neuroprotective activity, was synthesized on the basis of the FSUE at VolgSMU of the Ministry of Health of the Russian Federation. In accordance with the XIV State Pharmacopoeia of the Russian Federation, one of the criteria for quality control of pharmaceutical substances is the indicator "Related Impurities". Based on the synthesis scheme and the structure of the analyzed BAS as a technological impurity, we can assume the presence of the initial compound - - unsubstituted quinazoline-4(3H)-one, due to the possible incomplete flow of the reaction. Objective: the development and validation evaluation of the methodology for determining the technological impurity (quinazoline-4(3H)-one) to include it in the draft regulatory document for the proposed BAS VMA-13-15 as a pharmaceutical substance. Materials and methods. The capillary electrophoretic conditions proposed earlier by us for the determination of the BAS under study were chosen for the analysis, under which the compounds in question are well separated as clear peaks with a migration time of 6.59 min for N-[2-[4-oxo-3(4H)-quinazolinyl]propionyl]-guanidine and 4.37 min for quinazoline-4(3H)-one. Results. As a result of this research, the methodology for determining the indicator "Related Impurities" in BAS VMA-13-15 using capillary electrophoresis system was developed. The developed technique was validated in accordance with the requirements of SF RF of the XIV edition and proved to be acceptable for determination of the identified technological impurity quinazoline-4(3H)-one. Conclusion. The developed technique makes it possible to detect an impurity in the content of 0.1% or more.
{"title":"Development of a method for determining related impurities in a new biologically active compound of cardioprotective action by capillary electrophoresis","authors":"Evgeniya V. Kompantseva, Darya N. Lutsenko, E. R. Garcia, A. Ozerov, T. M. Dementieva","doi":"10.21626/vestnik/2023-2/09","DOIUrl":"https://doi.org/10.21626/vestnik/2023-2/09","url":null,"abstract":"The original biologically active compound (BAS) N-[2-[4-oxo-3(4H)-quinazolinyl]propionyl]-guanidine (VMA-13-15), which has cardioprotective and neuroprotective activity, was synthesized on the basis of the FSUE at VolgSMU of the Ministry of Health of the Russian Federation. In accordance with the XIV State Pharmacopoeia of the Russian Federation, one of the criteria for quality control of pharmaceutical substances is the indicator \"Related Impurities\". Based on the synthesis scheme and the structure of the analyzed BAS as a technological impurity, we can assume the presence of the initial compound - - unsubstituted quinazoline-4(3H)-one, due to the possible incomplete flow of the reaction. Objective: the development and validation evaluation of the methodology for determining the technological impurity (quinazoline-4(3H)-one) to include it in the draft regulatory document for the proposed BAS VMA-13-15 as a pharmaceutical substance. Materials and methods. The capillary electrophoretic conditions proposed earlier by us for the determination of the BAS under study were chosen for the analysis, under which the compounds in question are well separated as clear peaks with a migration time of 6.59 min for N-[2-[4-oxo-3(4H)-quinazolinyl]propionyl]-guanidine and 4.37 min for quinazoline-4(3H)-one. Results. As a result of this research, the methodology for determining the indicator \"Related Impurities\" in BAS VMA-13-15 using capillary electrophoresis system was developed. The developed technique was validated in accordance with the requirements of SF RF of the XIV edition and proved to be acceptable for determination of the identified technological impurity quinazoline-4(3H)-one. Conclusion. The developed technique makes it possible to detect an impurity in the content of 0.1% or more.","PeriodicalId":24060,"journal":{"name":"Курский научно-практический вестник «Человек и его здоровье»","volume":"13 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76822149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.21626/vestnik/2023-2/05
A. Yasenyavskaya, A. Tsibizova, L. Andreeva, N. Myasoedov, M. Samotrueva
Objective: to investigate immunotropic activity of the argenine-containing neuropeptide compound Lys-Lys-Arg-Arg-Pro-Gly-Pro in conditions of "social" stress. Material and methods. The study was conducted on mongrel male rats of 6 months of age using a model of "social" stress based on the provision of permanent residence of animals in conditions of sensory contact, as a result of which submissive and aggressive types of behavior were formed. All animals were divided into groups: control I - rats who were in a cage one at a time and received water for injection in an equivalent volume; control II - groups of stressed animals with aggressive and submissive types of behavior; three groups of stressed rats were intraperitoneally injected with Lys-Lys-Arg-Arg-Pro-Gly-Pro at a dose of 100 mcg/kg/day and the comparison drug Imunofan (Arginyl-alpha-aspartyl-lysyl-valyl-thyrosyl-argininum) at an average therapeutic dose of 0.7 mcg/kg/day. The following indicators were evaluated: delayed hypersensitivity reaction index, antibody titer of direct hemagglutination reaction, phagocytic activity of peripheral blood neutrophils, leukocyte formula indicators. Results. The results obtained in the course of this study indicate that the arginine-containing neuropeptide compound Lys-Lys-Arg-Arg-Pro-Gly-Pro contributes to the correction of immune changes that occur against the background of exposure to chronic "social" stress, namely, an increase in the delayed hypersensitivity reaction index, the level of anti-erythrocyte antibodies, the total number of leukocytes and a decrease in phagocytic activity of peripheral blood neutrophils (phagocytic number and phagocytic index), as well as restoration of the percentage ratio of elements of the leukocyte formula. Conclusion. Thus, the study of the immunotropic activity of the argenine-containing neuropeptide compound Lys-Lys-Arg-Arg-Pro-Gly-Pro showed the presence of an immunocorrecting effect on the cellular and humoral links of immunity, which actualizes the need for a detailed study of the immune mechanisms of this compound.
{"title":"Evaluation of the immunotropic activity of neuropeptide compound Lys-Lys-Arg-Arg-Pro-Gly-Pro in conditions of \"social\" stress","authors":"A. Yasenyavskaya, A. Tsibizova, L. Andreeva, N. Myasoedov, M. Samotrueva","doi":"10.21626/vestnik/2023-2/05","DOIUrl":"https://doi.org/10.21626/vestnik/2023-2/05","url":null,"abstract":"Objective: to investigate immunotropic activity of the argenine-containing neuropeptide compound Lys-Lys-Arg-Arg-Pro-Gly-Pro in conditions of \"social\" stress. Material and methods. The study was conducted on mongrel male rats of 6 months of age using a model of \"social\" stress based on the provision of permanent residence of animals in conditions of sensory contact, as a result of which submissive and aggressive types of behavior were formed. All animals were divided into groups: control I - rats who were in a cage one at a time and received water for injection in an equivalent volume; control II - groups of stressed animals with aggressive and submissive types of behavior; three groups of stressed rats were intraperitoneally injected with Lys-Lys-Arg-Arg-Pro-Gly-Pro at a dose of 100 mcg/kg/day and the comparison drug Imunofan (Arginyl-alpha-aspartyl-lysyl-valyl-thyrosyl-argininum) at an average therapeutic dose of 0.7 mcg/kg/day. The following indicators were evaluated: delayed hypersensitivity reaction index, antibody titer of direct hemagglutination reaction, phagocytic activity of peripheral blood neutrophils, leukocyte formula indicators. Results. The results obtained in the course of this study indicate that the arginine-containing neuropeptide compound Lys-Lys-Arg-Arg-Pro-Gly-Pro contributes to the correction of immune changes that occur against the background of exposure to chronic \"social\" stress, namely, an increase in the delayed hypersensitivity reaction index, the level of anti-erythrocyte antibodies, the total number of leukocytes and a decrease in phagocytic activity of peripheral blood neutrophils (phagocytic number and phagocytic index), as well as restoration of the percentage ratio of elements of the leukocyte formula. Conclusion. Thus, the study of the immunotropic activity of the argenine-containing neuropeptide compound Lys-Lys-Arg-Arg-Pro-Gly-Pro showed the presence of an immunocorrecting effect on the cellular and humoral links of immunity, which actualizes the need for a detailed study of the immune mechanisms of this compound.","PeriodicalId":24060,"journal":{"name":"Курский научно-практический вестник «Человек и его здоровье»","volume":"42 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90790332","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.21626/vestnik/2023-1/09
I. L. Drozdova, Daria D. Bogoslovskikh
The genus Sisymbrium L. of Brassicaceae family has up to 90 species of single and perennial herbaceous plants growing in temperate regions of both hemispheres. In Russia there are up to 20 species, of which the most common is Sisymbrium officinale (L.) Scop. This species is widely used in traditional medicine around the world as an expectorant, restorative, diuretic, anthelmintic, for diseases of the digestive and respiratory systems. It is included in the Pharmacopoeia of France. However, it is not included in the State Pharmacopoeia of Russia; the pharmacopoeia article for medicinal plant raw materials has not been approved. The possibility of using a new species in scientific medicine makes it necessary to study the morphological and anatomical structure of the Sisymbrium officinale. Objective: to perform morphological (external) and anatomical (internal) analysis of the structural features of the reproductive organs (flowers and fruits) of the medicinal herb; to establish the signs by which it is possible to make a reliable diagnosis. Materials and methods. The study of morphological and anatomical features of reproductive organs (flowers and fruits) of Sisymbrium officinale was carried out in accordance with the articles of the State Pharmacopoeia of the Russian Federation of the XIV edition. Freshly harvested, dried and fixed raw materials were used for the study. Results. For the first time macro- and microdiagnostic signs of reproductive organs (flowers and fruits) of Sisymbrium officinale were described. Conclusion. For the first time macroscopic and microscopic signs of reproductive organs (flowers and fruits) of Sisymbrium officinale have been identified and described, which can be used to determine the authenticity of raw materials, as well as to develop sections «External signs» and «Microscopic signs» of modern regulatory documentation.
{"title":"Morphological and anatomical structure of flowers and fruits of Sisymbrium officinale (L.) Scop.","authors":"I. L. Drozdova, Daria D. Bogoslovskikh","doi":"10.21626/vestnik/2023-1/09","DOIUrl":"https://doi.org/10.21626/vestnik/2023-1/09","url":null,"abstract":"The genus Sisymbrium L. of Brassicaceae family has up to 90 species of single and perennial herbaceous plants growing in temperate regions of both hemispheres. In Russia there are up to 20 species, of which the most common is Sisymbrium officinale (L.) Scop. This species is widely used in traditional medicine around the world as an expectorant, restorative, diuretic, anthelmintic, for diseases of the digestive and respiratory systems. It is included in the Pharmacopoeia of France. However, it is not included in the State Pharmacopoeia of Russia; the pharmacopoeia article for medicinal plant raw materials has not been approved. The possibility of using a new species in scientific medicine makes it necessary to study the morphological and anatomical structure of the Sisymbrium officinale. Objective: to perform morphological (external) and anatomical (internal) analysis of the structural features of the reproductive organs (flowers and fruits) of the medicinal herb; to establish the signs by which it is possible to make a reliable diagnosis. Materials and methods. The study of morphological and anatomical features of reproductive organs (flowers and fruits) of Sisymbrium officinale was carried out in accordance with the articles of the State Pharmacopoeia of the Russian Federation of the XIV edition. Freshly harvested, dried and fixed raw materials were used for the study. Results. For the first time macro- and microdiagnostic signs of reproductive organs (flowers and fruits) of Sisymbrium officinale were described. Conclusion. For the first time macroscopic and microscopic signs of reproductive organs (flowers and fruits) of Sisymbrium officinale have been identified and described, which can be used to determine the authenticity of raw materials, as well as to develop sections «External signs» and «Microscopic signs» of modern regulatory documentation.","PeriodicalId":24060,"journal":{"name":"Курский научно-практический вестник «Человек и его здоровье»","volume":"24 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90900145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.21626/vestnik/2023-2/11
N. N. Shvets, Yu.A. Sukhomlinov, R. Bubenchikov
Objective: study of the antiradical and antioxidant activity of water, water-alcohol and alcohol extracts obtained from alfalfa sickle grass. Materials and methods. As an object of research of the antiradical and antioxidant activities of water, water-alcohol and alcohol extracts of alfalfa sickle, the grass of alfalfa sickle in the flowering stage, which was harvested in the Russian Federation in the Kursk region, was considered. The antioxidant activity was evaluated using a titrimetric method based on the result of the interaction of substances of a reducing nature that are present in extracts from alfalfa sickle grass and potassium permanganate used as a titrant. Antiradical activity was studied by spectrophotometric method based on the ability of extracts to inactivate DPPH (2,2-diphenyl-1-picrylhydrazyl). The content of phenolic compounds (direct spectrophotometry) in terms of chlorogenic acid and flavonoids (differential spectrophotometry) in terms of rutin was determined. Statistical analysis was used to process the data obtained as a result of experiments. Results. Processing of the results of data obtained during experiments to determine the level of antioxidant and antiradical activity for various concentrations of aqueous, water-alcohol and alcohol extractants from alfalfa sickle grass confirmed the presence of such effects for all types of extracts studied. Under experimental conditions with the use of 30% ethanol as an extractant, the maximum atioxidant activity was observed at the level of 58.55%. The use of 96% ethanol as an extractant showed similarly the maximum antioxidant activity, at the level of 56.63%, which is slightly less, but significantly exceeds other indicators. The content of flavonoids was fixed at the maximum level (0.51%) when using 30% ethanol. Under the same conditions, phenolic compounds were recorded with an index of 0.88%. The results obtained visualize a direct correlation between the level of antioxidant activity of the studied object of the herb Medicago falcata L. and the level of phenolic compounds in it. As for the use of 96% ethanol as an extractant, then in this extraction the content of hydroxycinnamic acids and flavonoids was less (0.14% and 0.04% respectively). Conclusion. The resulting data from a series of experiments on alfalfa sickle grass collected during the flowering phase confirm the prospects of medicinal plant raw materials Medicago falcata L. as a raw material having antioxidant activity, which makes it possible to consider it as a basis for continuing pharmacological research.
{"title":"Study of the antioxidant activity of the herb alfalfa sickle (Medicago falcata L.)","authors":"N. N. Shvets, Yu.A. Sukhomlinov, R. Bubenchikov","doi":"10.21626/vestnik/2023-2/11","DOIUrl":"https://doi.org/10.21626/vestnik/2023-2/11","url":null,"abstract":"Objective: study of the antiradical and antioxidant activity of water, water-alcohol and alcohol extracts obtained from alfalfa sickle grass. Materials and methods. As an object of research of the antiradical and antioxidant activities of water, water-alcohol and alcohol extracts of alfalfa sickle, the grass of alfalfa sickle in the flowering stage, which was harvested in the Russian Federation in the Kursk region, was considered. The antioxidant activity was evaluated using a titrimetric method based on the result of the interaction of substances of a reducing nature that are present in extracts from alfalfa sickle grass and potassium permanganate used as a titrant. Antiradical activity was studied by spectrophotometric method based on the ability of extracts to inactivate DPPH (2,2-diphenyl-1-picrylhydrazyl). The content of phenolic compounds (direct spectrophotometry) in terms of chlorogenic acid and flavonoids (differential spectrophotometry) in terms of rutin was determined. Statistical analysis was used to process the data obtained as a result of experiments. Results. Processing of the results of data obtained during experiments to determine the level of antioxidant and antiradical activity for various concentrations of aqueous, water-alcohol and alcohol extractants from alfalfa sickle grass confirmed the presence of such effects for all types of extracts studied. Under experimental conditions with the use of 30% ethanol as an extractant, the maximum atioxidant activity was observed at the level of 58.55%. The use of 96% ethanol as an extractant showed similarly the maximum antioxidant activity, at the level of 56.63%, which is slightly less, but significantly exceeds other indicators. The content of flavonoids was fixed at the maximum level (0.51%) when using 30% ethanol. Under the same conditions, phenolic compounds were recorded with an index of 0.88%. The results obtained visualize a direct correlation between the level of antioxidant activity of the studied object of the herb Medicago falcata L. and the level of phenolic compounds in it. As for the use of 96% ethanol as an extractant, then in this extraction the content of hydroxycinnamic acids and flavonoids was less (0.14% and 0.04% respectively). Conclusion. The resulting data from a series of experiments on alfalfa sickle grass collected during the flowering phase confirm the prospects of medicinal plant raw materials Medicago falcata L. as a raw material having antioxidant activity, which makes it possible to consider it as a basis for continuing pharmacological research.","PeriodicalId":24060,"journal":{"name":"Курский научно-практический вестник «Человек и его здоровье»","volume":"20 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80282517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.21626/vestnik/2023-1/01
D. Yarovaya, O. Bashkina, Elena V. Krasilova
Coronavirus infection (COVID-19), caused by severe acute respiratory syndrome 2 (SARS-CoV-2) RNA virus, has become a global problem in the world. Its consequences are of great importance for the health status of both adults and children, since the number of lethal outcomes and life-threatening complications has reached high rates. Of particular importance is coronavirus damage of important organs in childhood, due to the possible impairment of their functions and the development of later distant complications. Thus, the formation of acute kidney injury (AKI) in children is an independent risk factor for hospital mortality and disability. An important role in the development of this pathological condition is given to the development of endothelial dysfunction due to the activation of a number of pro-inflammatory cytokines and other biomarkers of endothelial disorders. Endothelitis, endothelial injury, endothelial cell dysfunction, and impaired microcirculatory function in various vascular beds all contribute markedly to life-threatening complications of COVID-19 such as venous thromboembolism and multiple organ failure. Endothelial dysfunction is the main determinant of microvascular disorders due to a shift in vascular balance towards greater vasoconstriction and subsequent organ ischemia, inflammation with concomitant tissue edema and procoagulant condition. In general, understanding the mechanisms of endothelial activation and dysfunction during COVID-19 infection can contribute to early detection of individuals, especially children, at risk of severe complications. Further study of endothelial dysfunction in the pathogenesis of AKI in children with COVID-19 is promising to determine a personalized approach to the management of pediatric patients with COVID-19.
{"title":"Effects of coronavirus infection on markers of endothelial dysfunction in children with renal pathology","authors":"D. Yarovaya, O. Bashkina, Elena V. Krasilova","doi":"10.21626/vestnik/2023-1/01","DOIUrl":"https://doi.org/10.21626/vestnik/2023-1/01","url":null,"abstract":"Coronavirus infection (COVID-19), caused by severe acute respiratory syndrome 2 (SARS-CoV-2) RNA virus, has become a global problem in the world. Its consequences are of great importance for the health status of both adults and children, since the number of lethal outcomes and life-threatening complications has reached high rates. Of particular importance is coronavirus damage of important organs in childhood, due to the possible impairment of their functions and the development of later distant complications. Thus, the formation of acute kidney injury (AKI) in children is an independent risk factor for hospital mortality and disability. An important role in the development of this pathological condition is given to the development of endothelial dysfunction due to the activation of a number of pro-inflammatory cytokines and other biomarkers of endothelial disorders. Endothelitis, endothelial injury, endothelial cell dysfunction, and impaired microcirculatory function in various vascular beds all contribute markedly to life-threatening complications of COVID-19 such as venous thromboembolism and multiple organ failure. Endothelial dysfunction is the main determinant of microvascular disorders due to a shift in vascular balance towards greater vasoconstriction and subsequent organ ischemia, inflammation with concomitant tissue edema and procoagulant condition. In general, understanding the mechanisms of endothelial activation and dysfunction during COVID-19 infection can contribute to early detection of individuals, especially children, at risk of severe complications. Further study of endothelial dysfunction in the pathogenesis of AKI in children with COVID-19 is promising to determine a personalized approach to the management of pediatric patients with COVID-19.","PeriodicalId":24060,"journal":{"name":"Курский научно-практический вестник «Человек и его здоровье»","volume":"8 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85589399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.21626/vestnik/2023-2/06
A. Kaplin
Diabetes mellitus, as one of the most common socially significant diseases, has not lost its relevance for decades. Experimental models for studying this endocrinopathy are of scientific value not only in historical terms, but also greatly enrich modern ideas about various pathophysiological aspects of its course. Objective - to develop and test a new method for inducing diabetes mellitus (DM) with relative insulin deficiency for modeling gestational pathology. Materials and methods. The study was carried out on mature white female rats (30 individuals) of the Wistar line weighing 200-250 g. Using the method of pacreato-slenectomy described in the work, DM with relative insulin deficiency was induced in animals, after which ten female rats with hyperglycemia were transplanted to intact males. Results. Diabetes mellitus in laboratory animals was induced by removing the gastrosplenic part of the pancreas, which made it possible to reproduce the state of rather long-term hyperglycemia (up to 61 days of the experiment) with a high degree of both early and late survival. Female rats selected for the second stage of the experiment were able to become pregnant from intact males and carry offspring, while maintaining stable elevated blood glucose levels throughout the entire gestation period. Conclusion. The experimental model proposed by the authors can provide an opportunity for a long-term study of DM with relative insulin deficiency, as well as testing of new antidiabetic drugs, including against the background of pregnancy.
{"title":"New experimental model of diabetes mellitus for the study of gestational pathology","authors":"A. Kaplin","doi":"10.21626/vestnik/2023-2/06","DOIUrl":"https://doi.org/10.21626/vestnik/2023-2/06","url":null,"abstract":"Diabetes mellitus, as one of the most common socially significant diseases, has not lost its relevance for decades. Experimental models for studying this endocrinopathy are of scientific value not only in historical terms, but also greatly enrich modern ideas about various pathophysiological aspects of its course. Objective - to develop and test a new method for inducing diabetes mellitus (DM) with relative insulin deficiency for modeling gestational pathology. Materials and methods. The study was carried out on mature white female rats (30 individuals) of the Wistar line weighing 200-250 g. Using the method of pacreato-slenectomy described in the work, DM with relative insulin deficiency was induced in animals, after which ten female rats with hyperglycemia were transplanted to intact males. Results. Diabetes mellitus in laboratory animals was induced by removing the gastrosplenic part of the pancreas, which made it possible to reproduce the state of rather long-term hyperglycemia (up to 61 days of the experiment) with a high degree of both early and late survival. Female rats selected for the second stage of the experiment were able to become pregnant from intact males and carry offspring, while maintaining stable elevated blood glucose levels throughout the entire gestation period. Conclusion. The experimental model proposed by the authors can provide an opportunity for a long-term study of DM with relative insulin deficiency, as well as testing of new antidiabetic drugs, including against the background of pregnancy.","PeriodicalId":24060,"journal":{"name":"Курский научно-практический вестник «Человек и его здоровье»","volume":"20 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78943170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.21626/vestnik/2023-1/07
N. G. Sarkisyan, E. Gaisina, M. N. Dobrinskaya, Milana A. Shmakova, P. Astryukhina, Artyom S. Goskov
Studies (domestic and foreign) on the effect of vitamin D on muscle strength, muscle recovery rate, maximum oxygen consumption and prevention of skeletal muscle injury reduction have been analyzed. At the same time it was found that athletes are susceptible to hypovitaminosis no less often than standard population groups due to higher physical loads. It is unequivocally possible to say that vitamin D supplementation improves the physical fitness of athletes and can correct their condition in the autumn-spring period. Objective: an analysis of literature data based on the significance of the pharmacological effects of vitamin D in the functioning of the musculoskeletal system of the body and its role of improving athletic performance. Material and methods. The Pubmed/MEDLINE digital scientific library was chosen as the main source of information. Additionally, the search included the repositories of open access preprints from eLIBRARY.RU for 2016-2022. Key words for the search query were "vitamin D," "calcitriol," "sports," and "athletes." The results of the study are reported taking into account PRISMA recommendations. Results. Duration and quality of insolation directly affect vitamin D levels. Additional sun exposure combined with the use of vitamin D preparations contributes to the elimination of vitamin D deficiency. Vitamin D deficiency can lead to decreased intranuclear VDR concentrations and decreased VDR gene expression, which contributes to myopathy following atrophy of type IIA muscle fibers. Additional enrichment of special electrolyte solutions with water-soluble vitamin D is reasonable. Conclusion. Continuous monitoring of the concentration of 25(OH)D in the blood serum of athletes is an integral part in the development of recommendations for improving health and sports performance in training and competitive regimes. Individual correction of vitamin D should be carried out in accordance with changes in its level in each athlete.
{"title":"Literature review: pharmacological effects of vitamin D in the body to enhance athletic performance","authors":"N. G. Sarkisyan, E. Gaisina, M. N. Dobrinskaya, Milana A. Shmakova, P. Astryukhina, Artyom S. Goskov","doi":"10.21626/vestnik/2023-1/07","DOIUrl":"https://doi.org/10.21626/vestnik/2023-1/07","url":null,"abstract":"Studies (domestic and foreign) on the effect of vitamin D on muscle strength, muscle recovery rate, maximum oxygen consumption and prevention of skeletal muscle injury reduction have been analyzed. At the same time it was found that athletes are susceptible to hypovitaminosis no less often than standard population groups due to higher physical loads. It is unequivocally possible to say that vitamin D supplementation improves the physical fitness of athletes and can correct their condition in the autumn-spring period. Objective: an analysis of literature data based on the significance of the pharmacological effects of vitamin D in the functioning of the musculoskeletal system of the body and its role of improving athletic performance. Material and methods. The Pubmed/MEDLINE digital scientific library was chosen as the main source of information. Additionally, the search included the repositories of open access preprints from eLIBRARY.RU for 2016-2022. Key words for the search query were \"vitamin D,\" \"calcitriol,\" \"sports,\" and \"athletes.\" The results of the study are reported taking into account PRISMA recommendations. Results. Duration and quality of insolation directly affect vitamin D levels. Additional sun exposure combined with the use of vitamin D preparations contributes to the elimination of vitamin D deficiency. Vitamin D deficiency can lead to decreased intranuclear VDR concentrations and decreased VDR gene expression, which contributes to myopathy following atrophy of type IIA muscle fibers. Additional enrichment of special electrolyte solutions with water-soluble vitamin D is reasonable. Conclusion. Continuous monitoring of the concentration of 25(OH)D in the blood serum of athletes is an integral part in the development of recommendations for improving health and sports performance in training and competitive regimes. Individual correction of vitamin D should be carried out in accordance with changes in its level in each athlete.","PeriodicalId":24060,"journal":{"name":"Курский научно-практический вестник «Человек и его здоровье»","volume":"9 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84644949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.21626/vestnik/2023-2/01
V. Lazarenko, E. A. Bobrovskaya, I. S. Ivanov, V. Lipatov, V. Mishustin, Elizaveta I. Godunova
Objective: to assess the immune status of systemic and local blood flow after femoral-popliteal bypass surgery in patients with and without restenosis. Materials and methods. The analysis of the treatment of 82 patients who underwent femoral-popliteal bypass surgery was carried out. Depending on the condition of the reconstruction zone after 12 months, the patients were divided into two groups: group I - 21 patients without restenosis, group II - 61 patients with the development of restenosis. The analysis of IgA, IgM, IgG, circulating immune complexes (CIC), interleukin (IL)-6, IL-1 in the systemic and local blood flow before and after surgery was performed. Results. In patients with the development of restenosis of the reconstruction zone, the level of IgA in the systemic and IgA, IgM in the local bloodstream was initially lower, the level of CIC, IL-1, IL-6 in the systemic and IgG, IL-1, IL-6 in the local bloodstream was higher, after surgery, the level of IgA in the local bloodstream was lower, higher - IgM in systemic and IL-6 in local blood flow in comparison with patients without restenosis. The correlation between the immune status and the development of restenosis was established: before surgery in the systemic circulation - IgA (r=-0.31, p<0.01), CIC (r=0.39, p<0.001), IL-6 (r=0.25, p<0.05), IL-1 (r=0.26, p<0.05), in the local bloodstream - IgA (r=-0.26, p<0.05), IgM (r=-0.30, p<0.01), IgG (r=0.26, p<0.05), IL-6 (r=0.30, p<0.01), IL-1 (r=0.29, p<0.01); after surgery in the systemic circulation - IgM (r=0.33, p<0.01), in the local - IgA (r=-0.24, p<0.05), IL-6 (r=0.40, p<0.001). The relationship between the degree of chronic arterial insufficiency (r=0.22, p<0.05), the ankle- brachial index after surgery (r=-0.27, p<0.05) with the development of restenosis was revealed. Conclusion. The interrelation of the immune status of the systemic and local blood flow before and after surgery and restenosis in the anastomosis zone after femur-popliteal bypass surgery has been shown. The study of the immune status will make it possible to develop an algorithm for restenosis prevention.
{"title":"Immune status in developing restenosis in patients with occlusive-stenotic lesion of the femoral-popliteal arterial segment after femoral-popliteal bypass surgery","authors":"V. Lazarenko, E. A. Bobrovskaya, I. S. Ivanov, V. Lipatov, V. Mishustin, Elizaveta I. Godunova","doi":"10.21626/vestnik/2023-2/01","DOIUrl":"https://doi.org/10.21626/vestnik/2023-2/01","url":null,"abstract":"Objective: to assess the immune status of systemic and local blood flow after femoral-popliteal bypass surgery in patients with and without restenosis. Materials and methods. The analysis of the treatment of 82 patients who underwent femoral-popliteal bypass surgery was carried out. Depending on the condition of the reconstruction zone after 12 months, the patients were divided into two groups: group I - 21 patients without restenosis, group II - 61 patients with the development of restenosis. The analysis of IgA, IgM, IgG, circulating immune complexes (CIC), interleukin (IL)-6, IL-1 in the systemic and local blood flow before and after surgery was performed. Results. In patients with the development of restenosis of the reconstruction zone, the level of IgA in the systemic and IgA, IgM in the local bloodstream was initially lower, the level of CIC, IL-1, IL-6 in the systemic and IgG, IL-1, IL-6 in the local bloodstream was higher, after surgery, the level of IgA in the local bloodstream was lower, higher - IgM in systemic and IL-6 in local blood flow in comparison with patients without restenosis. The correlation between the immune status and the development of restenosis was established: before surgery in the systemic circulation - IgA (r=-0.31, p<0.01), CIC (r=0.39, p<0.001), IL-6 (r=0.25, p<0.05), IL-1 (r=0.26, p<0.05), in the local bloodstream - IgA (r=-0.26, p<0.05), IgM (r=-0.30, p<0.01), IgG (r=0.26, p<0.05), IL-6 (r=0.30, p<0.01), IL-1 (r=0.29, p<0.01); after surgery in the systemic circulation - IgM (r=0.33, p<0.01), in the local - IgA (r=-0.24, p<0.05), IL-6 (r=0.40, p<0.001). The relationship between the degree of chronic arterial insufficiency (r=0.22, p<0.05), the ankle- brachial index after surgery (r=-0.27, p<0.05) with the development of restenosis was revealed. Conclusion. The interrelation of the immune status of the systemic and local blood flow before and after surgery and restenosis in the anastomosis zone after femur-popliteal bypass surgery has been shown. The study of the immune status will make it possible to develop an algorithm for restenosis prevention.","PeriodicalId":24060,"journal":{"name":"Курский научно-практический вестник «Человек и его здоровье»","volume":"28 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80125271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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