Aquatic Toxicology最新文献

{"title":"Lithium enhanced plasmid-mediated conjugative transfer of antimicrobial resistance genes in Escherichia coli: Different concentrations and mechanisms","authors":"Jiaxing Li ,&nbsp;Dongzhe Sun ,&nbsp;Jiayi Wu ,&nbsp;Fen Liu ,&nbsp;Yaqi Xu ,&nbsp;Yuanhao Wang ,&nbsp;Xiaoxi Shui ,&nbsp;Qingyang Li ,&nbsp;Baohua Zhao","doi":"10.1016/j.aquatox.2025.107263","DOIUrl":"10.1016/j.aquatox.2025.107263","url":null,"abstract":"<div><div>Conjugative transfer, a pivotal mechanism in the transmission of antimicrobial resistance genes, is susceptible to various environmental pollutants. As an emerging contaminant, lithium (Li) has garnered much attention due to its extensive applications. This research investigated the effects of Li on conjugative transfer process, examining biochemical and omics perspectives. Results revealed that Li could increase the conjugative transfer frequency of both donor and recipient via different mechanisms at varying concentrations. At 0.1 mg/L LiCl, a notable increase in conjugative transfer frequency occurred without ROS elevation. However, the surge of ROS was identified as a crucial regulator at 100 mg/L LiCl, as eliminating ROS would significantly decrease the conjugative transfer frequency. Besides, comparative transcriptome analysis revealed consistent variations in “SOS response”, “quorum sensing” and “oxidative phosphorylation” pathways at both 0.1 mg/L and 100 mg/L LiCl concentrations, suggesting their pivotal roles as targets for Li regulation and is independent of Li concentration. While genes related to “conjugative transfer”, “pili”, “outer membrane protein” and “antioxidant enzyme” were only significantly regulated by 100 mg/L LiCl, possible to be the specific reasons for High (100 mg/L) LiCl increased conjugative transfer frequency. This study reveals the distinct effects and mechanisms of different concentration of Li on conjugative transfer in <em>E. coli</em>, providing a theoretical basis for the understanding of the environmental effects of Li.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"279 ","pages":"Article 107263"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143057615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sublethal effects of atrazine concentrations exposure on tadpoles of Dendropsophus minutus: Evaluation of redox status, micronuclei frequencies and comet assay as biomarkers","authors":"Alice Tâmara de Carvalho Lopes ,&nbsp;Marcelino Benvindo-Souza ,&nbsp;Daiany Folador Sotero ,&nbsp;Thays Millena Alves Pedroso ,&nbsp;Acácio Arlem Tomaz ,&nbsp;Andreya Gonçalves Costa ,&nbsp;Antônia Regina dos Santos Gois ,&nbsp;Thiago Bernardi Vieira ,&nbsp;Rogério Pereira Bastos ,&nbsp;Daniela de Melo e Silva","doi":"10.1016/j.aquatox.2025.107260","DOIUrl":"10.1016/j.aquatox.2025.107260","url":null,"abstract":"<div><div>Atrazine (ATZ) is an herbicide that can persist in terrestrial and aquatic environments and potentially cause significant harm to amphibian health. Therefore, the Brazilian National Environment Council (CONAMA) sets the limit concentration of ATZ in waters at 2μg/L. Our study evaluated the genotoxic, mutagenic, and biochemical alterations in <em>Dendropsophus minutus</em> tadpoles in the 25 Gosner stage, to acute exposure (96h) of ATZ (T1 - 0.02µg/L, T2 - 0.04µg/L, T3 - 0.08µg/L, T4 - 2µg/L). The comet assay showed all concentrations caused DNA damage with an increase to T2, T3, and T4. In the micronucleus test (MN) and Erythrocyte Nuclear Abnormality test (ENA), T3 and T4 accumulated more anucleated (AN), binucleated cells (BC) and ENAs. Redox imbalance was not detected. Therefore, we conclude that the concentrations tested are not safe for the health and development of <em>D. minutus</em> tadpoles, and the CONAMA limit needs to be reviewed since all tadpoles presented DNA damage. More studies are necessary to identify other alterations that ATZ can cause in the tadpole health of tropical species. Therefore, implementing public policies aimed at safeguarding the lives of both adult and juvenile amphibians is imperative for the conservation of biodiversity and ecosystem stability.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"279 ","pages":"Article 107260"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143072597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seasonality of cyanobacteria and eukaryotes in Lake Geneva and the impacts of cyanotoxins on growth of the model ciliate Tetrahymena pyriformis","authors":"Niveen Ismail ,&nbsp;Paul Seguin ,&nbsp;Lola Pricam ,&nbsp;Elisabeth M.L. Janssen ,&nbsp;Tamar Kohn ,&nbsp;Bas W. Ibelings ,&nbsp;Anna Carratalà","doi":"10.1016/j.aquatox.2025.107262","DOIUrl":"10.1016/j.aquatox.2025.107262","url":null,"abstract":"<div><div>Toxic cyanobacteria are likely to be favored by global warming and other human impacts, posing significant threats to aquatic ecosystems. While cyanobacterial blooms in eutrophic lakes are widely investigated, the dynamics of cyanobacteria and the effects of their toxins and bioactive metabolites on the plankton communities in mesotrophic and oligotrophic lakes are less well understood. Here we investigated seasonal dynamics of cyanobacteria, eukaryotic algae and cyanotoxins in oligo-mesotrophic Lake Geneva—the largest and deepest lake in western Europe. High-throughput sequencing of the 16S rRNA genes in 143 samples along a water column revealed that Lake Geneva hosts diverse, co-dominant cyanobacterial genera, including <em>Planktothrix, Cyanobium, Pseudanabaena</em>, and <em>Aphanizomenon.</em> The abundance of the <em>mcyA</em> gene marker for microcystin production was highly correlated with total cyanobacteria abundance, obtained from qPCR of the 16S rRNA genes. Targeted LC-HRMS/MS analysis demonstrated peak concentrations of cyanotoxins in September and December 2021 at the deep chlorophyll-a maximum layer, reaching up to 1474 ng/l for anabaenopeptins and 144 ng/l for microcystins. The toxin peaks did not correlate with the abundance or variations in the cyanobacteria or eukaryote community, but they were correlated in time with seasonal lows in the abundances of ciliates (18S rRNA analysis). Laboratory exposure tests demonstrated that growth of the model ciliate <em>Tetrahymena pyriformis</em> was inhibited by Microcystin-RR and Anabaenopeptin A at environmentally relevant concentrations in the ng/l-range, in natural lake water, synthetic freshwater, and growth media spiked with the cyanotoxins. Our findings suggest that even low concentrations (in the ng/l-range) of microcystins and anabaenopeptins, reduce growth of ciliates such as <em>T. pyriformis</em> and can be expected to have wider impacts on the eukaryote communities.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"279 ","pages":"Article 107262"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143072596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptional modulation in Mediterranean Mussel Mytilus galloprovincialis following exposure to four pharmaceuticals widely distributed in coastal areas","authors":"Ilaria Bernardini ,&nbsp;Marica Mezzelani ,&nbsp;Michela Panni ,&nbsp;Giulia Dalla Rovere ,&nbsp;Alessandro Nardi ,&nbsp;Ouafa El Idrissi ,&nbsp;Luca Peruzza ,&nbsp;Stefania Gorbi ,&nbsp;Serena Ferraresso ,&nbsp;Luca Bargelloni ,&nbsp;Tomaso Patarnello ,&nbsp;Francesco Regoli ,&nbsp;Massimo Milan","doi":"10.1016/j.aquatox.2025.107255","DOIUrl":"10.1016/j.aquatox.2025.107255","url":null,"abstract":"<div><div>Ecotoxicological risk and the mode of action of human drugs on non-target marine animals remain unclear, keeping a gap of knowledge on risks related to ecosystem disruption and chemical contamination of food chains.</div><div>Understanding these impacts is critical to developing proper waste management practices and regulatory frameworks to prevent long-term environmental and human health problems. This study investigates the impacts of Gemfibrozil, Metformin, Ramipril, and Venlafaxine, individually and combined on <em>Mytilus galloprovincialis</em> over 30 days and assesses persistent effects post-recovery using RNA-seq and 16S rRNA microbiota profiling. All pharmaceuticals caused few changes in the microbiota while gene expression analyses highlighted drug-specific alterations. Gemfibrozil exposure led to alterations in lipid and fatty acid metabolism, suggesting a similar mode of action to that observed in target species. Metformin significantly impacted the mussels' energy metabolism, with disruptions in specific genes and pathways potentially related to glucose uptake and insulin signaling. Metformin was also the treatment leading to the most significant changes in predicted functional profiles of the microbiota, suggesting that it may influence the microbiota's potential to interact with host glucose metabolism. Ramipril exposure resulted in the up-regulation of stress response and cell cycle regulation pathways and Venlafaxine induced changes in serotonin and synapse pathways, indicating potential similarities in mechanisms of action with target species. Mixture of the four pharmaceuticals severely impacted mussel physiology, including impairment of oxidative phosphorylation and compensatory activation of several pathways involved in energy metabolism. Despite recovery after depuration, changes in stress and energy related metabolism pathways suggests potential persistent effects from combined pharmaceutical exposure. Notably, the up-regulation of mTOR1 signaling in all treatments after 30 days underscores its key role in coordinating bivalve stress responses. The Transcriptomic Hazard Index (THI) calculated for each treatment indicates major/severe hazards after exposure that decreased to slight/moderate hazards after depuration.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"279 ","pages":"Article 107255"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143142723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physicochemical behavior and ecological risk of biofilm-mediated microplastics in aquatic environments","authors":"Ranran Zhou ,&nbsp;Xirong Huang ,&nbsp;Yongtao Ni,&nbsp;Zewei Ma,&nbsp;Hengchen Wei,&nbsp;Qijie Jin,&nbsp;Zhuhong Ding","doi":"10.1016/j.aquatox.2024.107209","DOIUrl":"10.1016/j.aquatox.2024.107209","url":null,"abstract":"<div><div>The prevalence of microplastics (MPs) in aquatic environments has become the core of environmental pollution. In recent years, the inevitable biological aging process of MPs in natural environments has attracted researchers’ attention. Such biofilm-mediated MPs, colonized by microorganisms, affect the physicochemical behavior and potential ecological risks of MPs. Therefore, it is critical to understand the impact of MPs’ biofilm formation on the environmental fate and toxicity of MPs. This review presented a comprehensive discussion of the impact of biofilm formation on unique carrier effects and toxicological effects of MPs in aquatic environments. First, the biofilm formation process on MPs, the compositions of microorganisms in biofilm and the factors influencing biofilm formation were briefly summarized. Second, the sorption of pollutants and enrichment of antibiotic resistance genes onto biofilm-mediated MPs were discussed. Third, the potential effects of biofilm-mediated MPs on gut microbiota were analyzed. Finally, gaps in the field that require further investigations were put forward. This review emphasized that biofilm-mediated MPs have higher environmental risks and ecotoxicity, which is helpful in providing new insights for pollution prevention and control of new pollutant MPs.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"279 ","pages":"Article 107209"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142870569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A multi-comprehensive approach to assess the responses of the Mediterranean mussel Mytilus galloprovincialis (Lamarck, 1919) to a simulation of a diesel-oil mixture spill","authors":"F. Bertini ,&nbsp;V. Catania ,&nbsp;L. Scirè Calabrisotto ,&nbsp;M. Dara ,&nbsp;L. Bisanti ,&nbsp;C. La Corte ,&nbsp;M. Staropoli ,&nbsp;D. Piazzese ,&nbsp;M.G. Parisi ,&nbsp;D. Parrinello ,&nbsp;M. Cammarata","doi":"10.1016/j.aquatox.2024.107188","DOIUrl":"10.1016/j.aquatox.2024.107188","url":null,"abstract":"<div><div>Oil spills are a major cause of pollution impacting marine ecosystems. In this work, the effects of short-term exposure to three different concentrations of a hydrocarbon mixture (HC), that simulated the action of such an event, were investigated on <em>Mytilus galloprovincialis</em> specimens. Physiological effects were measured using a battery of biomarkers consisting of cellular activity (phagocytosis), immune-related enzymes, chaperonins (HSP70 and HSC70), and histomorphological alterations. Different concentrations of HC led to a significant decrease in phagocytosis, especially following high concentrations. Immune-related enzymes evaluated in hemolymph and digestive gland extract showed up-regulation, suggesting the activation of antioxidant, detoxicant, and inflammatory responses. Morphological alterations of digestive gland tubules were observed after exposure to the HC. HSP70 and HSC70 activity was up regulated following the treatments, indicating their involvement in maintaining organism homeostasis. In addition, the diversity and composition of hemolymph and digestive gland microbiota exposed to HC were analyzed by automated ribosomal intergenic spacer analysis (ARISA) and a Next Generation Sequencing (NGS) approach to evaluate the connection with hydrocarbon contamination. Metagenomic analysis revealed significant differences in the hemolymph and digestive gland microbiota composition between mussels exposed and unexposed to HC. Exposure to increasing HC concentrations had a positive effect on microbial diversity with clear adaptative responses, and an increase in the relative abundance of several known degrading bacterial genera, including <em>Alcanivorax, Roseovarius, Pseudomonas, Vibrio, Oleibacter</em>. These results show the utility of a multi-comprehensive approach to evaluating functional adaptation in terms of immunological dysfunctions and microbiota alteration in the sentinel organism <em>M. galloprovincialis</em>.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"279 ","pages":"Article 107188"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142790002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of ammonia on the survival and physiology of juveniles and adults of Macrobrachium acanthurus (Caridea: Palaemonidae)","authors":"Esthephany K.S. Miranda ,&nbsp;Daniela P. Garçon ,&nbsp;Claudia C. Brazão ,&nbsp;Luis Carlos Ferreira de Almeida ,&nbsp;Giovana Bertini","doi":"10.1016/j.aquatox.2025.107261","DOIUrl":"10.1016/j.aquatox.2025.107261","url":null,"abstract":"<div><div>Water quality is a key factor influencing the survival and health of aquatic organisms. This study aims to assess the effects of ammonia exposure on <em>Macrobrachium acanthurus</em>, including juveniles, adult males, and ovigerous females. <em>Macrobrachium acanthurus</em> were exposed to total ammonia nitrogen (TAN) concentrations of 0, 20, 40, 60, and 80 mg L⁻¹ for 96 h to assess survival, total hemocyte count (THC), gill damage, and recovery capacity. The median lethal concentration (LC₅₀) of TAN after 96 h of exposure was 54.5 mg L⁻¹ (3.1 mg L⁻¹ NH₃) for juveniles, 50.4 mg L⁻¹ (2.9 mg L⁻¹ NH₃) for adult males, and 59.2 mg L⁻¹ (3.4 mg L⁻¹ NH₃) for ovigerous females. To avoid sub-lethal effects, TAN levels should remain below 5.0 mg L⁻¹ (0.3 mg L⁻¹ NH₃) at 26 °C and pH 8.0, ensuring animal welfare from the nursery stage onward. Reversible and moderate gill lesions occurred at 20 and 40 mg L⁻¹ of TAN, while 60 and 80 mg L⁻¹ resulted in irreversible damage. However, gill recovery was observed after exposure to 20 mg L⁻¹ of TAN. Concentrations above LC₅₀ affected innate immune response, regardless of sex, with changes in THC. These findings demonstrate the sensitivity of <em>M. acanthurus</em> to ammonia exposure and provide results of safe concentrations. Those results will contribute to species conservation and inform sustainable aquaculture practices for native freshwater species in Brazil.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"280 ","pages":"Article 107261"},"PeriodicalIF":4.1,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143147314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mass spectrometry imaging enables detection of MPs and their effects in Daphnia magna following acute exposure","authors":"Lidia Molina-Millán ,&nbsp;Eva Cuypers ,&nbsp;Laia Navarro-Martín ,&nbsp;Albert Menéndez-Pedriza ,&nbsp;Kimberly Garcia ,&nbsp;Marta Gual ,&nbsp;Carlos Barata ,&nbsp;Berta Cillero-Pastor ,&nbsp;Ron M.A. Heeren","doi":"10.1016/j.aquatox.2025.107253","DOIUrl":"10.1016/j.aquatox.2025.107253","url":null,"abstract":"<div><div>Microplastics (MPs) are continuously found in soil and water environments. Within aquatic ecosystems, filter-feeding organisms are unable to discriminate MPs from food particles while fish may intentionally ingest MPs by mistaking them for prey. In both cases, MPs can accumulate in tissues with subsequent implications for human and environmental health. The modes of action of MPs are still not fully understood and hence the toxicological effects of these pollutants cannot be fully evaluated. This study aims to improve our understanding of the modes of action and toxicological effects of MPs using a multimodal approach. In the present study, <em>Daphnia magna</em> was deployed as a model to investigate the acute effects of MPs by exposing <em>D. magna</em> specimens for 24 h to fluorophore-coated polyethylene MPs. A multimodal approach, combining fluorescence imaging and mass spectrometry imaging (MSI), was employed to assess the implications of MPs exposures. Fluorescent microscopy revealed a significant accumulation of MPs in the gut of <em>D. magna</em> after acute exposure. Secondary ion mass spectrometry (SIMS) and matrix-assisted laser desorption/ionization (MALDI) imaging were used to study the distribution and potential metabolic effects in exposed <em>D. magna</em>. ToF-SIMS revealed specific fragmentation patterns for polyethylene MPs, with the <em>m/z</em> 43 ion being the most suitable for identifying polyethylene MPs in biological tissue samples. MALDI-MSI showed specific ion types for the eye, gut, optical ganglion, first antennae, and egg tissues of <em>D. magna</em>. MSI data revealed minor alterations in specific regions of <em>D. magna</em>, such as eggs and gut, of <em>D. magna</em> after MPs exposure. The local changes were mainly found in the nucleotide and lipid metabolism within the eggs. In the gut, changes between control and MPs-exposed groups were potentially linked to plastic additives. Overall, the results of this work underline the potential of multimodal approaches based on MSI to study challenging pollutants, such as MPs, and their interactions with tissues.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"280 ","pages":"Article 107253"},"PeriodicalIF":4.1,"publicationDate":"2025-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143147311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of steroid hormones and their mixtures on western mosquitofish (Gambusia affinis)","authors":"Chen-Si Wang ,&nbsp;Guo-Yong Huang ,&nbsp;Dong-Qiao Lei ,&nbsp;Guang-Guo Ying","doi":"10.1016/j.aquatox.2024.107167","DOIUrl":"10.1016/j.aquatox.2024.107167","url":null,"abstract":"<div><div>Steroid hormones, including estrone (E1), androstadienedione (ADD), and androstenedione (AED), are prevalent in aquatic ecosystems and pose ecological risks due to their disruptive influence on fish populations. However, little consideration has been given to the endocrine disrupting effects of fish exposed to complex mixtures of hormones in the real world. In this study, adult female western mosquitofish (<em>Gambusia affinis</em>) were exposed to two concentrations of E1 (100 ng/L for E1L and 5,000 ng/L for E1H), ADD (100 ng/L for ADDL and 10,000 ng/L for ADDH), and AED (100 ng/L for AEDL and 10,000 ng/L for AEDH) as well as four binary mixture treatments (ADDL+E1L, ADDH+E1H, AEDL+E1L, and AEDH+E1H). After 42 d, their basic physiological parameters, secondary sex characteristics, gonadal health, embryo numbers, and HPG axis-related gene expression were evaluated. Results showed that the P/D ratio of hemal spines in AEDH+E1H exhibited a pronounced reduction, approximately half that of E1H. Moreover, the number of embryos in ADDH+E1H and AEDH+E1H was reduced by approximately 3-fold compared to E1H. Correspondingly, <em>G. affinis</em> exposure to ADDH+E1H and AEDH+E1H increased the proportion of degenerated oocytes. Exposure to combined treatments led to significant changes in the transcription of HPG axis-related genes in fish and displayed a certain degree of interaction. Furthermore, cluster heatmap analysis of target genes demonstrated that ADD+E1 and AED+E1 (both high and low concentrations) were far apart from ADD, AED and E1. Collectively, these observations imply the presence of antagonistic interactions in combined treatments, and the negative impact on the growth, maturation, and endocrine system of <em>G. affinis</em> varies accordingly.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"278 ","pages":"Article 107167"},"PeriodicalIF":4.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142724554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of functional groups of polystyrene nanoplastics on the neurodevelopmental toxicity of acrylamide in the early life stage of zebrafish","authors":"Haohan Yang,&nbsp;Linghui Kong,&nbsp;Zhuoyu Chen,&nbsp;Jun Wu","doi":"10.1016/j.aquatox.2024.107177","DOIUrl":"10.1016/j.aquatox.2024.107177","url":null,"abstract":"<div><div>Polystyrene nanoplastics (PS NPs) and acrylamide (ACR), both emerging contaminants, have been found to be related to neurotoxicity. However, the effects of PS NPs on ACR-induced neurodevelopmental toxicity remain unclear. In this study, anionic carboxyl polystyrene nanoplastics (PS NPs-COOH), cationic amino polystyrene nanoplastics (PS NPs-NH₂) and unmodified PS NPs were selected to investigate their interaction with ACR. A serious of the neurotoxicity biomarkers from individual to molecular level were evaluated to explore the specific mechanisms. The results indicated that the unmodified PS NPs had the most significant impact on embryonic development at low concentrations in combination with ACR. The toxicity of the other two functionalized PS NPs increased with concentration, exhibiting a clear dose-response relationship. Meanwhile, all three kinds of PS NPs significantly enhanced the impacts of ACR on the locomotion behavior of zebrafish larvae. Analysis of zebrafish nervous system development showed that PS NPs-COOH exhibit greater toxicity to the central nervous system. In contrast, PS NPs-NH₂ had a more significant impact on the motor nervous system. Gene expression analysis revealed that ACR and PS NPs significantly affected the expression levels of neurodevelopmental related genes, including <em>Neurog1, Elavl3, Gfap, Gap43, Mbpa, Shha</em>. PS NPs modified with functional groups could induce corresponding neurotoxicity by affecting genes expression related to neuronal differentiation, motor neuron, and axonal development. Based on the comprehensive biomarker response index, the order of the impacts of NPs on the neurotoxicity of ACR was PS NPs-COOH &gt; PS NPs-NH₂ &gt; PS NPs. In conclusion, this study provides new insights into the interactive biological effects of NPs and ACR on zebrafish embryo, contributing to a better understanding of their environmental risk to aquatic ecosystem.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"278 ","pages":"Article 107177"},"PeriodicalIF":4.1,"publicationDate":"2024-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142744410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}