Aquatic Toxicology最新文献_第8页

Prolonged curcumin supplementation causes tissue-specific antioxidant responses in adult oysters: Potential implications for resilience against abiotic and biotic stressors in the aquaculture industry

IF 4.1 2区环境科学与生态学 Q1 MARINE & FRESHWATER BIOLOGY

Aquatic Toxicology

Pub Date : 2025-02-06 DOI: 10.1016/j.aquatox.2025.107282

Heloísa Bárbara Gabe , Karine Amabile Taruhn , Danielle Ferraz Mello , Melody Lebrun , Christine Paillard , Charlotte Corporeau , Alcir Luiz Dafre , Rafael Trevisan

Aquatic animals inhabiting marine coastal environments are highly susceptible to environmental fluctuations and pollution, exemplified by widespread mass mortalities induced by marine bacteria or viruses. Enhancing antioxidant defenses presents a promising strategy to mitigate such environmental stressors. We postulated that supplementation of oysters with natural compounds such as flavonoids, exemplified by curcumin (CUR), could effectively bolster their antioxidant protection. Adult Pacific oysters were supplemented with CUR (30 μM) in seawater for 2, 4, 8, and 16 days. CUR metabolites progressively accumulated in gills, mantle, and digestive glands. Notably, oyster antioxidant response was significantly augmented, as evidenced by elevated glutathione (GSH) levels, and enhanced activities of glutathione reductase (GR), thioredoxin reductase (TrxR), and glutathione S-transferase (GST) after 4, 8, and 16 days of CUR supplementation. This response was tissue-specific, with the most pronounced increase in gills, followed by mantle, whereas digestive gland exhibited minimal response. After being supplemented with CUR for 8 days, oysters were subjected to antioxidant-disrupting agents such as N-ethylmaleimide (NEM), 1‑chloro-2,4-dinitrobenzene (CDNB). Both chemicals reduced antioxidant protection in untreated animals. However, CUR supplementation prevented these redox-disrupting effects, suggesting the potential ability of CUR to counteract antioxidant stressors. The effects of 8 days of CUR supplementation were also tested against the lethal effects of the pathogens V. tapetis, V, alginolyticus, and V. anguillarum, but CUR failed to induce immunological protection. The antioxidant protection induced by CUR holds promise for application in aquaculture to bolster animal health and resilience against abiotic stressors. Further research is needed to investigate the long-term impact of CUR supplementation and its role against biotic stressors, such as bacterial and viral infections.

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引用次数: 0

Influence of CYP1A and AhR modulation on polycyclic aromatic hydrocarbon-induced developmental defects in Japanese medaka

IF 4.1 2区环境科学与生态学 Q1 MARINE & FRESHWATER BIOLOGY

Aquatic Toxicology

Pub Date : 2025-02-05 DOI: 10.1016/j.aquatox.2025.107267

Shusaku Fukugami , Masatoshi Yamasaki , Emiko Kokushi , Seiichi Uno

Polycyclic aromatic hydrocarbons (PAHs) are known to induce developmental malformations in fish embryos. However, the interaction between aryl hydrocarbon receptor (AhR) and cytochrome P450 (CYP) in PAH-induced development defects remains unclear. Therefore, we investigated the effects of the CYP1A inhibitor piperonylbutoxide (PBO) and the AhR antagonist CH223191 (CH) on the development of Japanese medaka (Oryzias Latipes) embryos exposed to different PAHs. Japanese medaka embryos were exposed to three conditions: PAH alone, PAH and PBO, and PAH and CH. Microscopic observations were performed to examine the presence of developmental defects. Although neither phenanthrene (Phe) nor fluoranthene (Flu) induced morphological malformations in larvae, benzo(a)anthracene (BaA) exposure induced craniofacial deformities in the larvae. Additionally, BaA and PBO co-exposure significantly increased the rate and severity of malformations. Pyrene (Pyr) exposure induced craniofacial defects, cardiac hypertrophy, pericardial edema, and spinal curvature, which were attenuated by exposure to either CH or PBO. Collectively, these findings suggest that structurally different PAHs exert their toxic effects via distinct mechanisms during fish development.

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引用次数: 0

The key molecular mechanisms of antagonism induced by combined exposure to erythromycin and roxithromycin in Chlorella pyrenoidosa

IF 4.1 2区环境科学与生态学 Q1 MARINE & FRESHWATER BIOLOGY

Aquatic Toxicology

Pub Date : 2025-02-04 DOI: 10.1016/j.aquatox.2025.107269

Feifan Wu , Xiangjie Pan , Yuhao Zhou , Yan Zhu , Kai Liu , Wei Li , Jiangang Han

Emerging pollutants such as antibiotics have raised great concern in recent years, but the complex coexistence of multiple antibiotics in the environment poses a new challenge in the accurate assessment of the toxicity of antibiotics to aquatic organisms such as microalgae. In this study, the mechanism of action of a combination of erythromycin (ERY) and roxithromycin (ROX) on Chlorella pyrenoidosa was illustrated based on the physiological–biochemical response and transcriptomic analysis. The results revealed an inhibitory effect on the biomass of C. pyrenoidosa at 14 d in all treatment groups, whereas an antagonistic effect was observed in the coexposure groups. The photosystem was the main target despite the existence of multiple compensatory mechanisms, such as expanding the antenna size and initiating alternative electron carriers. The intercept of electrons on the donor side of PSI limited the production of energy, whereas the adjustment of the content and ratio of pigments strengthened microalgal adaptation. Enzymes and genes related to the degradation of exogenous compounds, including cytochrome P450 (P450), glutathione S-transferase (GST) and ABC transporters, mediated the detoxification of antibiotics. The upregulated expression of related genes induced by coexposure increased resistance and explained the antagonistic effects. The shift in energy allocation by increasing the proportion of lipids met the urgent requirements of microalgal physiological activities. This study reemphasizes the modes of interactions between multiple antibiotics and provides new insights into the mechanisms of antagonism induced by combinations of antibiotics.

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引用次数: 0

Vitamin C alleviates intestinal damage induced by 17α-methyltestosterone in Carassius auratus

IF 4.1 2区环境科学与生态学 Q1 MARINE & FRESHWATER BIOLOGY

Aquatic Toxicology

Pub Date : 2025-02-02 DOI: 10.1016/j.aquatox.2025.107266

Haiyan Zhao , Tongyao Li , Chenyang Li , Zijun Xiong , Weiya Rong , Lu Cao , Gen Chen , Qing Liu , Yu Liu , Xianzong Wang , Shaozhen Liu

17α-Methyltestosterone (MT), an environmental endocrine-disrupting chemical (EDC), is widely present in aquatic environments, posing potential threats to the health of aquatic organisms. This study aimed to explore the protective effects of Vitamin C (VC) against MT-induced intestinal injury in Carassius auratus and evaluate the optimal VC dosage. C. auratus were exposed to 50 ng/L MT with 0, 25, 50, and 150 mg/kg VC for 7, 14, and 21 d. Intestinal pathological changes were assessed using paraffin sections, digestive enzyme activity was measured, RT-qPCR was used to analyze the expression of genes related to the intestinal barrier and inflammation, and 16S rDNA sequencing was conducted to evaluate the intestinal flora. MT exposure caused villus damage, vacuolization, and free lymphocytes. Additionally, it upregulated TNF-α and Claudin-12 expression and downregulated IL-10, Occludin, and Muc2 expression, exacerbating intestinal inflammation, damaging barrier function, and reducing digestive enzyme activity. VC at 25 and 50 mg/kg significantly alleviated MT-induced damage by restoring villus length and mitigating the downregulation of anti-inflammatory factors and tight junction protein-related genes while inhibiting TNF-α mRNA expression. 16S rDNA sequencing revealed that MT disrupted the intestinal flora and increased the abundance of harmful bacteria such as Acinetobacter, whereas VC promoted Lactobacillus production and enhanced digestive enzyme activity. We hypothesize that MT exposure promotes the growth of harmful bacteria in the intestines, leading to inflammation and damage to the mucosal barrier. 25 and 50 mg/kg VC can mitigate MT-induced intestinal injury by regulating the intestinal microbiota and have potential protective effects.

{"title":"Vitamin C alleviates intestinal damage induced by 17α-methyltestosterone in Carassius auratus","authors":"Haiyan Zhao , Tongyao Li , Chenyang Li , Zijun Xiong , Weiya Rong , Lu Cao , Gen Chen , Qing Liu , Yu Liu , Xianzong Wang , Shaozhen Liu","doi":"10.1016/j.aquatox.2025.107266","DOIUrl":"10.1016/j.aquatox.2025.107266","url":null,"abstract":"<div><div>17α-Methyltestosterone (MT), an environmental endocrine-disrupting chemical (EDC), is widely present in aquatic environments, posing potential threats to the health of aquatic organisms. This study aimed to explore the protective effects of Vitamin C (VC) against MT-induced intestinal injury in <em>Carassius auratus</em> and evaluate the optimal VC dosage. <em>C. auratus</em> were exposed to 50 ng/L MT with 0, 25, 50, and 150 mg/kg VC for 7, 14, and 21 d. Intestinal pathological changes were assessed using paraffin sections, digestive enzyme activity was measured, RT-qPCR was used to analyze the expression of genes related to the intestinal barrier and inflammation, and 16S rDNA sequencing was conducted to evaluate the intestinal flora. MT exposure caused villus damage, vacuolization, and free lymphocytes. Additionally, it upregulated <em>TNF-α</em> and <em>Claudin-12</em> expression and downregulated <em>IL-10, Occludin,</em> and <em>Muc2</em> expression, exacerbating intestinal inflammation, damaging barrier function, and reducing digestive enzyme activity. VC at 25 and 50 mg/kg significantly alleviated MT-induced damage by restoring villus length and mitigating the downregulation of anti-inflammatory factors and tight junction protein-related genes while inhibiting <em>TNF-α</em> mRNA expression. 16S rDNA sequencing revealed that MT disrupted the intestinal flora and increased the abundance of harmful bacteria such as <em>Acinetobacter</em>, whereas VC promoted <em>Lactobacillus</em> production and enhanced digestive enzyme activity. We hypothesize that MT exposure promotes the growth of harmful bacteria in the intestines, leading to inflammation and damage to the mucosal barrier. 25 and 50 mg/kg VC can mitigate MT-induced intestinal injury by regulating the intestinal microbiota and have potential protective effects<em>.</em></div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"280 ","pages":"Article 107266"},"PeriodicalIF":4.1,"publicationDate":"2025-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143147315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Combined effects of polymethylmethacrylate microplastics with arsenic and copper on the euryhaline rotifer Proales similis 聚甲基丙烯酸甲酯微塑料与砷和铜对广盐轮虫的联合作用。

IF 4.1 2区环境科学与生态学 Q1 MARINE & FRESHWATER BIOLOGY

Aquatic Toxicology

Pub Date : 2025-02-01 DOI: 10.1016/j.aquatox.2024.107214

Uriel Arreguin-Rebolledo , Federico Páez-Osuna , Gladys Valencia-Castañeda , Mario Alberto Arzate-Cárdenas , Mariana V. Capparelli

Aquatic organisms are typically exposed to chemical mixtures, including microplastics and metal(loid)s. However, most research has primarily focused on the toxicity of individual chemicals, potentially overestimating their risks to aquatic life. This study examined the combined effects of polymethylmethacrylate microplastics (PMMA-MPs) with As and Cu at environmentally relevant concentrations on the euryhaline rotifer Proales similis. The 48-h EC₅₀ of PMMA-MPs (1.5–11.5 μm) was determined using concentrations from 1.0 to 100 mg/L. These concentrations were then combined with 0.41 mg/L As or 0.034 mg/L Cu. The combined effects of PMMA-MPs (0.031–1.0 mg/L) with As (0.025–0.2 mg/L) and Cu (0.0025–0.02 mg/L) at environmental concentrations were assessed through five-day chronic toxicity reproductive tests. The population growth rate was the endpoint for both acute and chronic testing. The EC₅₀ of PMMA-MPs for P. similis was 44.0 mg/L, but this value significantly decreased when PMMA-MPs were co-exposed with the tested metal(loid)s. The tested mixtures of PMMA-MPs with As and Cu reduced growth rates by 13 – 48 % and 11 – 35 %, respectively, compared to the individual exposure to each chemical. This indicates that PMMA-MPs alter the toxic behavior of these metal(loid)s and vice versa. The present findings reveal that the combination of environmentally relevant concentrations of MPs (modeled as PMMA-MPs) with As and Cu can induce synergistic effects in marine rotifers. These results highlight the importance of investigating pollution in realistic scenarios that many aquatic invertebrates face.

水生生物通常暴露于化学混合物中，包括微塑料和金属（胶体）。然而，大多数研究主要集中在个别化学品的毒性上，可能高估了它们对水生生物的风险。本研究考察了聚甲基丙烯酸甲酯微塑料（PMMA-MPs）与环境相关浓度的砷和铜对泛盐轮虫的联合作用。采用1.0 ~ 100 mg/L浓度测定PMMA-MPs (1.5 ~ 11.5 μm) 48h EC50。然后将这些浓度与0.41 mg/L的砷或0.034 mg/L的铜混合。通过5天慢性毒性生殖试验，评估环境浓度下PMMA-MPs （0.031-1.0 mg/L）与砷（0.025-0.2 mg/L）和铜（0.0025-0.02 mg/L）的联合效应。人口增长率是急性和慢性试验的终点。PMMA-MPs的EC50值为44.0 mg/L，但当PMMA-MPs与所测金属（样物质）共暴露时，EC50值显著降低。测试的PMMA-MPs与As和Cu的混合物与单独暴露于每种化学物质相比，分别降低了13 - 48%和11 - 35%的生长速率。这表明PMMA-MPs改变了这些金属（类）s的毒性行为，反之亦然。本研究结果表明，环境相关浓度的MPs（建模为PMMA-MPs）与as和Cu的结合可以诱导海洋轮虫的协同效应。这些结果强调了在许多水生无脊椎动物面临的现实情况下调查污染的重要性。

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引用次数: 0

Histopathological and histochemical analysis of the digestive tract of adult horseshoe crabs under mercury stress 汞胁迫下成年鲎消化道的组织病理学和组织化学分析

IF 4.1 2区环境科学与生态学 Q1 MARINE & FRESHWATER BIOLOGY

Aquatic Toxicology

Pub Date : 2025-02-01 DOI: 10.1016/j.aquatox.2024.107202

Wanling Zhang , Mujiao Xie , Xiaohai Chen , Ziwei Ying , Yuyuan Bao , Yang-Guang Gu , Xiaoyong Xie

Mercury (Hg) contamination is a major environmental concern that continuously impacts marine organisms, including the mangrove horseshoe crab (Carcinoscorpius rotundicauda). As a vulnerable species, C. rotundicauda holds significant ecological and evolutionary value, making it particularly susceptible to Hg pollution and deserving of focused conservation efforts. This study presents the first comprehensive histopathological and histochemical analysis of mercury toxicity in the digestive tract of adult C. rotundicauda. Through both qualitative and quantitative analyses of histopathological and histochemical alterations, we evaluated the effects of acute Hg exposure (0.5 mg/L and 5 mg/L) on the esophagus, stomach, and intestines over time. The results revealed significant dose- and time-dependent tissue damage, with the stomach exhibiting the most pronounced alterations, including epithelial detachment, vacuolation caused by fibers degradation in the loose connective tissue, and muscle layer disruption, followed by the esophagus and intestines. Additionally, mucous cell density in the hindgut submucosa decreased by 30 % after 96 h of acute Hg exposure (0.5 and 5 mg/L HgCl₂), indicating a weakened protective barrier. The tissue damage of the digestive tract may further have a negative impact on the health of the adult horseshoe crab, thus threatening the survival of C. rotundicauda population and reducing the biodiversity of the mangrove ecosystem. These findings underscore the critical threat posed by Hg pollution to the digestive system of C. rotundicauda and offer novel insights into the toxicological impact on marine invertebrates. Considering the tissue-damaging effects of Hg on adult horseshoe crabs, this study underscores the importance of regular monitoring of Hg contamination in natural habitats. The results offer valuable guidance for future habitat restoration and effective management of existing habitats.

汞污染是一个主要的环境问题，不断影响海洋生物，包括红树林马蹄蟹（Carcinoscorpius rotundicauda）。作为一种易危物种，圆尾螺具有重要的生态和进化价值，对汞污染尤为敏感，值得重点保护。本研究提出的第一个全面的组织病理学和组织化学分析汞毒性在消化道的成年rotundicauda。通过组织病理学和组织化学变化的定性和定量分析，我们评估了急性汞暴露（0.5 mg/L和5 mg/L）对食管、胃和肠道的影响。结果显示了明显的剂量和时间依赖性组织损伤，胃表现出最明显的改变，包括上皮脱离，疏松结缔组织纤维降解引起的空泡化，肌肉层破坏，其次是食道和肠道。此外，在急性汞暴露（0.5和5 mg/L HgCl2） 96小时后，后肠粘膜下层的粘膜细胞密度下降了30%，表明保护屏障减弱。消化道的组织损伤可能进一步对成年马蹄蟹的健康产生负面影响，从而威胁到圆尾蟹种群的生存，降低红树林生态系统的生物多样性。这些发现强调了汞污染对圆尾螺消化系统的严重威胁，并为海洋无脊椎动物的毒理学影响提供了新的见解。考虑到汞对成年马蹄蟹的组织损伤作用，本研究强调了在自然栖息地定期监测汞污染的重要性。研究结果为今后生境恢复和现有生境的有效管理提供了有价值的指导。

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引用次数: 0

Polystyrene microplastics exacerbate acetochlor-induced reproductive toxicity and transgenerational effects in zebrafish 聚苯乙烯微塑料加剧乙氯诱导的斑马鱼生殖毒性和跨代效应

IF 4.1 2区环境科学与生态学 Q1 MARINE & FRESHWATER BIOLOGY

Aquatic Toxicology

Pub Date : 2025-02-01 DOI: 10.1016/j.aquatox.2024.107208

Yang Wang , Chaofan Ma , Zenglong Chen , Yinping Guo , Yuan Jing , Xiaolei Chen , Hongwu Liang

Microplastic (MPs) can adsorb co-existing pollutants, and alter their behavior and toxicity. Meanwhile, amide herbicides like acetochlor (ACT) are widely used in agriculture, with potential endocrine-disrupting effects that raise ecological concerns. The aim of this research was to examine the effects of MPs on the reproductive endocrine disruption caused by ACT and the effects of maternal transmission. Zebrafish were employed in this study to assess the reproductive toxicity of ACT alone and in combination with polystyrene microplastics (PS-MPs) of different sizes (200 nm and 2 μm) and concentrations (0.1 and 1 mg/L) over a 63-day exposure experiment. The results indicated that ACT was concentrated in zebrafish tissues in the order: intestine > liver > gill > brain > gonad > muscle. PS-MPs increased ACT bioaccumulation, worsened gonadal damage, led to abnormalities in hormone levels, and caused disruptions in HPG axis gene expression, further exacerbating the reproductive toxicity. Maternal transfer of ACT affected offspring growth, thyroid function, and HPT axis gene expression, with nanoplastics (NPS) amplifying these adverse effects. This study offers crucial insights into the ecological hazards posed by ACT and PS-MPs, emphasizing the increased toxicity due to PS-MPs.

微塑料（MPs）可以吸附共存的污染物，并改变它们的行为和毒性。与此同时，乙草胺（ACT）等酰胺类除草剂被广泛用于农业，其潜在的内分泌干扰效应引起了生态问题。本研究的目的是检查MPs对ACT引起的生殖内分泌紊乱的影响以及母体传播的影响。本研究利用斑马鱼进行了为期63天的暴露实验，以评估ACT单独暴露和不同尺寸（200 nm和2 μm）、不同浓度（0.1和1 mg/L）的聚苯乙烯微塑料（PS-MPs）的生殖毒性。结果表明，ACT在斑马鱼组织中的富集顺序为：肠；肝脏在吉尔比;大脑比;性腺祝辞肌肉。PS-MPs增加ACT生物蓄积，加重性腺损伤，导致激素水平异常，破坏HPG轴基因表达，进一步加剧生殖毒性。母体转移ACT影响后代生长、甲状腺功能和HPT轴基因表达，而纳米塑料（NPS）放大了这些不利影响。这项研究对ACT和PS-MPs的生态危害提供了重要的见解，强调了PS-MPs引起的毒性增加。

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引用次数: 0

Cetacean species found stranded along Croatian coast of the Adriatic Sea as bioindicators of non-essential trace elements in the environment 在克罗地亚亚得里亚海沿岸发现搁浅的鲸类动物，作为环境中非必需微量元素的生物指标

IF 4.1 2区环境科学与生态学 Q1 MARINE & FRESHWATER BIOLOGY

Aquatic Toxicology

Pub Date : 2025-02-01 DOI: 10.1016/j.aquatox.2024.107206

Marija Sedak , Maja Đokić , Nina Bilandžić , Tomislav Gomerčić , Miroslav Benić , Manuela Zadravec , Martina Đuras

In tissues of toothed whales from the Adriatic Sea (muscle, liver, kidney, lung, spleen, adipose tissue and skin) the concentrations of cadmium (Cd), lead (Pb) and arsenic (As) were analysed. In total, 186 dolphins were analysed; 155 bottlenose (Tursiops truncatus), 25 striped (Stenella coeruleoalba) and 6 Risso's dolphins (Grampus griseus). Cadmium concentrations in tissue samples ranged from 0.001 mg/kg in muscle to 16.8 mg/kg wet weight in kidney. Arsenic concentrations in dolphin samples ranged from 0.010 to 12.9 mg/kg ww. The lowest As concentration was found in spleen and highest in liver of bottlenose dolphin. Cadmium and As levels in Risso's dolphins showed higher concentrations in all tissues in comparison to bottlenose and striped dolphins. >50 % of the measured Pb values for all three species of dolphins and examined tissues were lower than 0.1 mg/kg. The accumulation of Cd and As during the lifetime was confirmed. None of the dolphins analysed in this study were exposed to concentrations of Cd in the liver higher than 20 mg/kg wet weight, which can cause renal failure in marine mammals. Numerous species of marine mammals inhabit coastal environments alongside humans and utilize similar food sources, such as fish and cephalopods. Consequently, these mammals can function as valuable indicators of public health concerns.

分析了亚得里亚海齿鲸组织（肌肉、肝脏、肾脏、肺、脾脏、脂肪组织和皮肤）中镉（Cd）、铅（Pb）和砷（As）的浓度。总共分析了186只海豚；155只宽吻海豚（Tursiops truncatus）， 25只条纹海豚（Stenella coeruleoalba）和6只里索海豚（grpus griseus）。组织样品中的镉浓度范围从肌肉中的0.001 mg/kg到肾脏中的16.8 mg/kg湿重。海豚样本中的砷浓度为0.010至12.9 mg/kg ww。宽吻海豚的砷含量以脾脏最低，肝脏最高。与宽吻海豚和条纹海豚相比，里索海豚体内的镉和砷含量在所有组织中的浓度都更高。所有三种海豚和被检查组织中50%的铅测量值低于0.1 mg/kg。证实了镉和砷在其一生中的积累。在这项研究中分析的海豚中，没有一只暴露在肝脏中镉浓度高于20毫克/公斤湿重的环境中，这可能导致海洋哺乳动物肾功能衰竭。许多种类的海洋哺乳动物与人类一起生活在沿海环境中，并利用类似的食物来源，如鱼类和头足类动物。因此，这些哺乳动物可以作为公共卫生问题的有价值的指标。

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引用次数: 0

Dietary chlorogenic acid supplementation protects against lipopolysaccharide-induced oxidative stress, inflammation and apoptosis in intestine of amur ide (Leuciscus waleckii) 饲粮中添加绿原酸对豚鼠肠道脂多糖诱导的氧化应激、炎症和细胞凋亡具有保护作用。

IF 4.1 2区环境科学与生态学 Q1 MARINE & FRESHWATER BIOLOGY

Aquatic Toxicology

Pub Date : 2025-02-01 DOI: 10.1016/j.aquatox.2024.107223

Yintao Wang , Sitong Meng , Denglai Li , Siying Liu , Liang LI , Lifang Wu

In this study, the alleviative effects of chlorogenic acid (CGA) on oxidative stress, inflammation and apoptosis of amur ide (Leuciscus waleckii) induced by lipopolysaccharide (LPS) were evaluated. Using a 2 × 2 factorial design, amur ide were irregularity divided into 4 groups and fed two diets with 0.00 % (CK and LPS), 0.04 % CGA(CGA and LC). After 4-week feeding trial, LPS challenge was executed. Results showed that 0.04 % CGA alleviated LPS-induced intestinal barrier dysfunction by decreasing the levels of 5-HT, D-LA, ET-1 and DAO in serum, increasing ZO-1, Occludin-α, Claudin-c, Claudin-f mRNA, and ZO-1, Occludin, Claudin-1 protein expression, improving intestinal morphology. Moreover, 0.04 % CGA alleviated LPS-induced inflammation and apoptosis by up-regulating TGF-β and Bcl-2 mRNA, down-regulating NF-κBp65, TNF-α, Bax, Caspase-3, Caspase-9 mRNA and NF-κBp65, Bax, Caspase-3 protein expression. 0.04 %CGA reversed LPS-induced the reduction of GSH-PX, CAT, T-SOD and T-AOC in intestines, whereas MDA showed the opposite result. 0.04 % alleviated LPS-induced the decrease of Nrf2, HO-1, CAT, SOD mRNA and Nrf2 protein expression, the increase of Keap1 mRNA. Summary, this study suggested that 0.04 % of dietary CGA alleviated LPS-induced intestinal oxidative stress, inflammation and apoptosis of amur ide.

本研究探讨了绿原酸（CGA）对脂多糖（LPS）诱导的白桦（Leuciscus waleckii）氧化应激、炎症和凋亡的缓解作用。采用2 × 2因子设计，随机分为4组，分别饲喂0.00% （CK + LPS）和0.04% CGA（CGA + LC）的饲粮。饲喂4周后，进行LPS攻毒。结果表明，0.04% CGA可通过降低血清5-HT、D-LA、ET-1和DAO水平，提高ZO-1、Occludin-α、Claudin-c、Claudin-f mRNA和ZO-1、Occludin、Claudin-1蛋白表达，改善肠道形态，减轻lps诱导的肠道屏障功能障碍。0.04% CGA通过上调TGF-β和Bcl-2 mRNA，下调NF-κBp65、TNF-α、Bax、Caspase-3、Caspase-9 mRNA和NF-κBp65、Bax、Caspase-3蛋白表达，减轻lps诱导的炎症和凋亡。0.04% CGA能逆转lps诱导的肠内GSH-PX、CAT、T-SOD和T-AOC的降低，而MDA则相反。0.04%可减轻lps诱导的Nrf2、HO-1、CAT、SOD mRNA和Nrf2蛋白表达的降低，Keap1 mRNA表达的升高。综上所述，本研究提示，饲粮中添加0.04%的CGA可减轻lps诱导的小鼠肠道氧化应激、炎症和凋亡。

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引用次数: 0

Response of the photosynthetic physiology of Ulva lactuca to Cu toxicity under ocean acidification 海洋酸化条件下海藻光合生理对Cu毒性的响应。

IF 4.1 2区环境科学与生态学 Q1 MARINE & FRESHWATER BIOLOGY

Aquatic Toxicology

Pub Date : 2025-02-01 DOI: 10.1016/j.aquatox.2024.107222

Tianpeng Xu , Zhouyue Lu , Cheng Chen , Yuxin Xie , Jing Ma , Juntian Xu

Ocean acidification can significantly affect the physiological performance of macroalgae. While copper (Cu) is an essential element for macroalgae and has been extensively studied, the interactive effects of ocean acidification and Cu on these organisms remain less understood. In this study, we measured the photosynthetic characteristics of Ulva lactuca exposed to varying Cu concentrations at two CO₂ levels (415 ppmv, low concentration; 1000 ppmv, high concentration). The results indicated that during chronic toxicity testing, the growth of juvenile U. lactuca significantly increased at Cu concentrations of 0.001 μM, 0.01 μM, and 0.1 μM regardless of low CO₂ concentrations or high CO₂ concentrations condition. In acute toxicity tests, elevated Cu concentrations negatively impacted the growth rate, yield, and photosynthetic rate of U. lactuca under low CO₂ concentrations. Conversely, high CO₂ concentrations enhanced the photosynthetic capacity of U. lactuca with increased Cu concentrations, while the growth rate significantly decreased at Cu concentration of 1.5 μM. Additionally, the activities of peroxidase (POD) and ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) increased, with an enhancement of malondialdehyde (MDA) content at 1.5 μM Cu under high CO₂ conditions. However, the structure of the chloroplast thylakoid was disrupted by elevated Cu concentrations. These findings suggest that low Cu concentrations promote the growth of U. lactuca, whereas high Cu concentrations inhibit algal growth, and ocean acidification may exacerbate the adverse effects of Cu on U. lactuca in acute toxicity tests.

海洋酸化会显著影响大型藻类的生理性能。虽然铜（Cu）是大型藻类的必需元素，并已被广泛研究，但海洋酸化和Cu对这些生物的相互作用仍然知之甚少。在本研究中，我们测量了在两个CO2水平(415 ppmv，低浓度；1000 ppmv，高浓度)。结果表明：在慢性毒性试验中，无论低CO2浓度还是高CO2浓度，铜浓度分别为0.001 μM、0.01 μM和0.1 μM时，乳藻幼鱼的生长均显著增加；在急性毒性试验中，在低CO2浓度下，Cu浓度升高对U. lactuca的生长速率、产量和光合速率有负面影响。相反，随着Cu浓度的增加，高CO2浓度可以增强U. lactuca的光合能力，而在Cu浓度为1.5 μM时，生长速率显著降低。此外，在1.5 μM Cu浓度下，随着丙二醛（MDA）含量的增加，过氧化物酶（POD）和核酮糖-1,5-二磷酸羧化酶/加氧酶（Rubisco）活性增加。然而，叶绿体类囊体的结构被Cu浓度升高所破坏。这些结果表明，低Cu浓度可促进乳藻的生长，而高Cu浓度可抑制藻类的生长，并且在急性毒性试验中，海洋酸化可能加剧Cu对乳藻的不良影响。

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