Aquatic Toxicology最新文献_第8页

Identification of Fentin chloride -induced scoliosis in zebrafish through the regulation of adrenaline by metabolomics and network pharmacology 通过代谢组学和网络药理学鉴定氯化芬丁对肾上腺素的调节对斑马鱼脊柱侧凸的影响

IF 4.3 2区环境科学与生态学 Q1 MARINE & FRESHWATER BIOLOGY

Aquatic Toxicology

Pub Date : 2025-11-03 DOI: 10.1016/j.aquatox.2025.107635

Hao Cheng , Shanshan Zhu , Wenyan Liu , Fasheng Liu , Jing Hu , Xinwei Xu , Xinjun Liao , Xiaowen Shi , Huiqiang Lu

Fentin chloride (TPTC) is an organotin compound used extensively in hull construction due to its excellent resistance to corrosion. To explore the biotoxicity of TPTC, In this study, the molecular mechanism of TPTC-induced scoliosis was investigated in the zebrafish model by combining metabolomics and network pharmacology. TPTC exposure causes scoliosis and neurotoxicity in zebrafish, and leads to increasing curvature angles with the increase in concentration. Metabolomic analysis identified 366 significant differential metabolites associated with TPTC-induced scoliosis. Network pharmacology revealed 32 intersecting targets between TPTC and scoliosis, and Kyoto Encyclopedia of Genes and Genomes pathway analysis demonstrated that these targets were mainly enriched in pathways, such as, neuroreceptor-ligand interactions, cGMP-PKG signaling, and adrenergic signaling. The combined analysis identified adrenergic receptors as the key targets of TPTC. ELISA revealed increased levels of adrenaline with the increase in TPTC concentrations. RT-qPCR showed that the expression levels of the Urotensin-Related Peptide genes (Urp1, Urp2) and their receptor genes (Utrs2r1), which regulate body axis curvature, are significantly increased downstream of the adrenaline signaling pathway. The use of an adrenaline inhibitor mitigated TPTC-induced scoliosis and the level of Urp1, Urp2 and Utrs2r1 nearly recovered to normal levels. Therefore, TPTC induces scoliosis by acting on adrenergic receptors to interfere with epinephrine and up-regulating the expression of downstream Urotensin-Related Peptide genes.

氯化芬丁（TPTC）是一种有机锡化合物，因其优异的耐腐蚀性而广泛应用于船体结构中。为了探讨TPTC的生物毒性，本研究采用代谢组学和网络药理学相结合的方法，在斑马鱼模型中研究TPTC诱导脊柱侧凸的分子机制。TPTC暴露导致斑马鱼脊柱侧凸和神经毒性，并导致弯曲角度随着浓度的增加而增加。代谢组学分析确定了366种与tptc诱导的脊柱侧凸相关的显著差异代谢物。网络药理学揭示了TPTC与脊柱侧凸之间的32个交叉靶点，京都基因与基因组百科全书通路分析表明，这些靶点主要富集于神经受体-配体相互作用、cGMP-PKG信号通路和肾上腺素能信号通路。综合分析发现肾上腺素能受体是TPTC的关键靶点。ELISA结果显示肾上腺素水平随着TPTC浓度的升高而升高。RT-qPCR结果显示，在肾上腺素信号通路下游，调节体轴曲率的尿紧张素相关肽基因（Urp1、Urp2）及其受体基因（Utrs2r1）的表达水平显著升高。肾上腺素抑制剂的使用减轻了tptc诱导的脊柱侧凸，Urp1、Urp2和Utrs2r1的水平几乎恢复到正常水平。因此，TPTC通过作用于肾上腺素能受体，干扰肾上腺素，上调下游尿紧张素相关肽基因的表达，从而诱导脊柱侧凸。

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引用次数: 0

Endocrine disruptive effects and mechanisms of isothiazolinone analogs in marine medaka (Oryzias melastigma) embryos and H295R cells 异噻唑啉酮类似物对海洋medaka胚胎和H295R细胞的内分泌干扰作用及其机制

IF 4.3 2区环境科学与生态学 Q1 MARINE & FRESHWATER BIOLOGY

Aquatic Toxicology

Pub Date : 2025-11-01 DOI: 10.1016/j.aquatox.2025.107633

Jiali Li , Lizhu Tang , Jing Li , Chenyan Hu , Jiahui Cao , Wenbing Zhang , Lianguo Chen

The application of isothiazolinones as antimicrobial agents has caused prevalent pollution of marine environment. However, the developmental toxicity and endocrine disrupting effects of different isothiazolinone analogs to marine organisms remain largely elusive. In the present study, marine medaka embryos were exposed to 0, 1, 3, and 10 μg/L of six representative isothiazolinone analogs, including isothiazole, methylisothiazolinone (MIT), methylchloroisothiazolinone (MCI), octylisothiazolinone (OIT), benzisothiazolinone (BIT), and dichlorocthylisothiazolinone (DCOIT), after which the anomalies in early embryogenesis and endocrine homeostasis were examined. In vivo results showed that OIT, BIT, and DCOIT were potently estrogenic, which was characterized by the higher estradiol (E₂) content and remarkable elevation of E₂/testosterone ratio in larval medaka. Correlation analysis found significantly positively relationship among OIT, BIT, and DCOIT, indicating their similar modes of toxicity. Besides, in vitro exposure using H295R cells was performed to gain more insights into endocrine disrupting mechanisms, further confirming the multiple disturbances of DCOIT in reproductive endocrine cascades, consisting of MAPK proteins, aromatase, and sex hormones. Overall, the present findings underscore the high susceptibility of marine organisms to the developmental and endocrine toxicity of isothiazolinones, especially during early embryonic stages. In the future, the marine ecological risks of isothiazolinones need to be evaluated urgently, thus facilitating chemical lifecycle management.

异噻唑啉酮类抗菌药物的应用对海洋环境造成了普遍的污染。然而，不同的异噻唑啉酮类似物对海洋生物的发育毒性和内分泌干扰作用在很大程度上仍然是难以捉摸的。本研究采用0、1、3、10 μg/L的异噻唑啉酮、甲基异噻唑啉酮（MIT）、甲基氯异噻唑啉酮（MCI）、辛基异噻唑啉酮（OIT）、苄基异噻唑啉酮（BIT）、二氯辛基异噻唑啉酮（DCOIT）等6种代表性异噻唑啉酮类似物对海洋medaka胚胎进行了早期胚胎发育异常和内分泌稳态的检测。体内实验结果表明，OIT、BIT和DCOIT具有强雌激素作用，其特点是雌二醇（E2）含量较高，E2/睾酮比显著升高。相关分析发现，OIT、BIT和DCOIT三者之间呈显著正相关，表明它们的毒性模式相似。此外，利用H295R细胞进行体外暴露，进一步了解内分泌干扰机制，进一步证实DCOIT在由MAPK蛋白、芳香化酶和性激素组成的生殖内分泌级联中的多重干扰。总的来说，目前的研究结果强调了海洋生物对异噻唑啉酮的发育和内分泌毒性的高度敏感性，特别是在早期胚胎阶段。未来，迫切需要对异噻唑啉酮类化合物的海洋生态风险进行评估，从而促进化学品生命周期管理。

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引用次数: 0

Biochemical and molecular stress responses to enrofloxacin in Mytilus galloprovincialis: transient or lasting effects? 对恩诺沙星的生化和分子应激反应：短暂或持久的影响？

IF 4.3 2区环境科学与生态学 Q1 MARINE & FRESHWATER BIOLOGY

Aquatic Toxicology

Pub Date : 2025-10-29 DOI: 10.1016/j.aquatox.2025.107626

J. Giannessi , C. Pretti , V. Meucci , L. Intorre , L. De Marchi , B. Gabbrielli , M. Baratti

Antibiotic contamination is an emerging threat in marine environments due to its potential to alter key physiological processes in non-target organisms. Among veterinary antibiotics, enrofloxacin (ENR), a widely used fluoroquinolone, is frequently detected in coastal waters. This study investigated the biological effects of ENR exposure in the Mediterranean mussel Mytilus galloprovincialis using an integrated approach combining gene expression, biochemical, and bioenergetic endpoints. Mussels were exposed to two environmentally relevant concentrations of ENR (5 and 500 ng/L) for 7 days (EXP7) and 14 days (EXP14), followed by a 14-day recovery period (REC). Gill tissues were analyzed for transcriptional changes in genes related to detoxification, oxidative stress, apoptosis, and energy metabolism. In addition, energy reserves, mitochondrial activity, and indicators of genotoxicity and antioxidant responses were measured. Results showed that ENR exposure modulated the expression of several genes, including early upregulation of abcb and cyp4y1, and downregulation of pkpyr and icdh, indicating activation of detoxification pathways and energy metabolism shifts. Biochemical analyses revealed increased mitochondrial activity (ETS), mobilization of energy reserves, and sustained elevation of GST activity, while DNA strand break levels confirmed a potential genotoxic effect. Despite the removal of the contaminant, most stress responses did not fully revert during recovery. These findings demonstrate that even low concentrations of ENR can disrupt mussel physiology at multiple levels, with effects persisting beyond exposure. The study underscores the importance of incorporating sub-lethal biomarkers into environmental risk assessments and supports the inclusion of veterinary antibiotics like ENR in marine monitoring programs to protect coastal ecosystem health.

抗生素污染是海洋环境中一个新兴的威胁，因为它有可能改变非目标生物的关键生理过程。在兽用抗生素中，恩诺沙星（ENR）是一种广泛使用的氟喹诺酮类药物，在沿海水域中经常被检测到。本研究采用结合基因表达、生化和生物能量终点的综合方法，研究了ENR暴露对地中海贻贝（Mytilus galloprovincialis）的生物学效应。将贻贝暴露于两种环境相关浓度的ENR（5和500 ng/L）中7天（EXP7）和14天（EXP14），然后进行14天的恢复期（REC）。分析鳃组织中与解毒、氧化应激、细胞凋亡和能量代谢相关的基因的转录变化。此外，还测量了能量储备、线粒体活性、遗传毒性和抗氧化反应指标。结果表明，ENR暴露可调节多个基因的表达，包括abcb和cyp4y1的早期上调，pkpyr和icdh的下调，表明解毒途径的激活和能量代谢的改变。生化分析显示线粒体活性（ETS）增加，能量储备动员，GST活性持续升高，而DNA链断裂水平证实了潜在的遗传毒性作用。尽管去除了污染物，但大多数应激反应在恢复过程中并没有完全恢复。这些发现表明，即使是低浓度的ENR也会在多个层面上破坏贻贝的生理机能，其影响持续到暴露后。该研究强调了将亚致死生物标志物纳入环境风险评估的重要性，并支持将兽医抗生素（如ENR）纳入海洋监测计划，以保护沿海生态系统的健康。

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引用次数: 0

Marine ecotoxicity evaluation of 10 per- and poly-fluoroalkyl acids using three USEPA short-term chronic bioassays 使用三种USEPA短期慢性生物测定法评价10种单氟和多氟烷基酸的海洋生态毒性

IF 4.3 2区环境科学与生态学 Q1 MARINE & FRESHWATER BIOLOGY

Aquatic Toxicology

Pub Date : 2025-10-28 DOI: 10.1016/j.aquatox.2025.107625

Nicholas T. Hayman , Zacharias Pandelides , Josiah Discar , Marienne A. Colvin , Gunther Rosen , Jennifer Arblaster , Jason Conder

Effects data for per- and poly-fluoroalkyl substances (PFAS) for marine aquatic life are lacking, limiting the assessment of site-specific ecological risks in marine ecosystems and development of marine water quality criteria. In this study, the toxicity of 10 PFAS (PFBA, PFHxA, PFOA, PFDA, PFBS, PFHxS, PFOS, PFDS, 6:2 FTS, and 8:2 FTS) were evaluated with three standard marine toxicity testing species: Mediterranean mussel (Mytilus galloprovincialis); purple sea urchin (Strongylocentrotus purpuratus); and giant kelp (Macrocystis pyrifera). Many tests failed to elicit responses exceeding 50 % adverse effect levels, despite approaching solubility limits. EC₅₀ values were able to be derived in only 43 % of the toxicity tests, and were generally above 1 mg/L, except for PFOS and PFDA based on the most sensitive of the three species (S. purpuratus and M. galloprovincialis), with EC₅₀s in the approximate 0.1 to 1 mg/L range. M. pyrifera, the macroalgae, was less sensitive than either invertebrate species. EC₅₀ values for all three species decreased with perfluoroalkyl carbon chain length, as increasing toxicity was observed in longer chained compounds, and a statistically significant relationship between chain length and EC₅₀ was detected for M. galloprovincialis. A number of other toxicological metrics (NOECs, LOECs, EC₁₀s, and EC₂₀s) were also generated. NOECs for sublethal endpoints were in the 0.01 to 100 mg/L range and were orders of magnitude higher than environmentally relevant concentrations. Based on the results in this study, adverse effects on these species and endpoints would not be expected to occur in the PFAS-impacted marine environment.

缺乏单氟烷基物质和多氟烷基物质对海洋水生生物的影响数据，限制了对海洋生态系统中特定地点生态风险的评估和海洋水质标准的制定。在这项研究中,10个pfa的毒性(PFBA PFHxA, PFOA、PFDA可以,PFHxS,卵圆孔未闭,pdf, 6:2 FTS,宣告FTS)进行评估和三个标准的海洋物种毒性测试:地中海贻贝(Mytilus galloprovincialis);紫海胆（strongylocentrrotus purpuratus）；巨藻（Macrocystis pyrifera）。尽管接近溶解度极限，但许多试验未能引起超过50%的不良反应水平。只有43%的毒性试验能够得出EC50值，一般都在1毫克/升以上，除了全氟辛烷磺酸和全氟辛烷磺酸，这是基于三种物种中最敏感的一种（紫癜脓毒杆菌和加洛省脓毒杆菌），EC50值大约在0.1至1毫克/升之间。大型藻类M. pyrifera的敏感性低于两种无脊椎动物。所有三种物种的EC50值都随着全氟烷基碳链长度的增加而降低，因为观察到链长化合物的毒性增加，并且在链长度与加galloprovincialis的EC50之间检测到具有统计学意义的关系。还生成了许多其他毒理学指标（noec、loec、ec10和ec20）。亚致死终点noec在0.01至100 mg/L范围内，比环境相关浓度高几个数量级。根据本研究的结果，在受pfas影响的海洋环境中，预计不会对这些物种和端点产生不利影响。

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引用次数: 0

Effects of pyrethroid insecticides on sex ratios in agile frogs (Rana dalmatina) 拟除虫菊酯类杀虫剂对敏捷蛙性比的影响

IF 4.3 2区环境科学与生态学 Q1 MARINE & FRESHWATER BIOLOGY

Aquatic Toxicology

Pub Date : 2025-10-28 DOI: 10.1016/j.aquatox.2025.107624

Emese Balogh , Szabolcs Hócza , Nikolett Ujhegyi , Andrea Kásler , Dóra Holly , Dávid Herczeg , János Ujszegi , Zoltán Gál , Orsolya I. Hoffmann , Veronika Bókony , Zsanett Mikó

Environmental pollutants have the potential to alter sex ratios in wildlife through sex-biased mortality. Furthermore, endocrine disruptors may cause sex reversal during early ontogeny in ectothermic vertebrates, resulting in a phenotypic sex that is not concordant with the genotypic sex encoded by the sex chromosomes. Despite the wide-ranging implications of these sex-ratio biasing effects, they are rarely studied in ecotoxicology, especially in a way that allows for disentangling the two mechanisms. We investigated these effects of two synthetic pyrethroids, deltamethrin and etofenprox, that are commonly used insecticides and have been linked to adverse effects on fish and amphibian biodiversity. We assessed the effects of environmentally relevant concentrations of these two pyrethroids on phenotypic sex ratio, sex-dependent mortality, and sex reversal in agile frogs (Rana dalmatina). Tadpoles from field-collected eggs were reared in mesocosms until metamorphosis by adding 0.03 or 0.3 μg/L of deltamethrin or etofenprox three times to the water. We observed no effect in three of the four treatment groups. However, in the lower-concentration etofenprox treatment, phenotypic sex ratio was male-biased two months post-metamorphosis, and genotypic sexing revealed that this was due to female-biased mortality during metamorphosis and not to sex reversal. Although the estimation certainty of these effects was somewhat limited, they highlight that not all sex-ratio distorting effects are caused by sex reversal. Therefore, ecotoxicological studies aiming to understand the endocrine distruptor effects of environmental contaminants should strive to separate the effects on sex determination and sex-dependent mortality.

环境污染物有可能通过性别偏向性死亡率改变野生动物的性别比例。此外，内分泌干扰物可能在变温脊椎动物的早期个体发育过程中引起性别逆转，导致表现型性别与性染色体编码的基因型性别不一致。尽管这些性别比例偏倚效应具有广泛的影响，但它们很少在生态毒理学中进行研究，特别是以一种允许分离这两种机制的方式。我们研究了两种合成拟除虫菊酯（溴氰菊酯和乙醚菊酯）的这些影响，这两种杀虫剂是常用的，并与对鱼类和两栖动物生物多样性的不利影响有关。我们评估了这两种拟除虫菊酯的环境相关浓度对敏捷蛙（Rana dalmatina）表型性比、性别依赖性死亡率和性别逆转的影响。将田间采卵的蝌蚪置于中胚层中饲养，分别在水中添加0.03或0.3 μg/L的溴氰菊酯或乙醚菊酯3次，直至蜕变。我们观察到四个治疗组中有三个没有效果。然而，在低浓度的乙托芬prox治疗中，表型性别比例在变态后两个月偏向于男性，基因型性别分析显示，这是由于变态期间的死亡率偏向于女性，而不是性别逆转。虽然这些影响的估计确定性有些有限，但他们强调，并非所有的性别比例扭曲效应都是由性别逆转引起的。因此，生态毒理学研究旨在了解环境污染物对内分泌干扰物的影响，应努力将其对性别决定和性别依赖性死亡率的影响分开。

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引用次数: 0

Molecular responses to pollution stress in glass eels (Anguilla anguilla): Gene expression changes associated with varying contamination levels and temperature across estuaries 玻璃鳗（Anguilla Anguilla）对污染应激的分子反应：与河口污染水平和温度变化相关的基因表达变化

IF 4.3 2区环境科学与生态学 Q1 MARINE & FRESHWATER BIOLOGY

Aquatic Toxicology

Pub Date : 2025-10-28 DOI: 10.1016/j.aquatox.2025.107623

Alvaro Gutierrez-Rodriguez , Claudia Nuñez-Moyano , Lucía Rivas-Iglesias , Susana Acle , Luis Jose Royo-Martin , Eva Garcia-Vazquez , Gonzalo Machado-Schiaffino

Understanding the effects of global change is crucial to aid in the conservation of critically endangered species such as the European eel (Anguilla anguilla). Emerging pollutants (microplastics) and legacy ones such as heavy metals, as well as thermal pollution, are deleterious for this species; however, their molecular impacts on glass eels entering estuaries remain understudied. Investigating the molecular signatures of environmental stress can provide insights into some of the factors contributing to A. anguilla population decline. In this study, expression changes in candidate genes associated with general and thermal stress (hsp70 & hsp90), oxidative stress (sod1, sod2, cat, gpx1), heavy metal detoxification (mt2), and neurotoxicity (ache) were measured in gill tissue of glass eels exposed to polystyrene (PS) microbeads in an aquarium, and also in individuals sampled from six Bay of Biscay estuaries with different levels of microplastics, heavy metals, nitrites, nitrates and thermal pollution. In the aquarium experiment, a significant positive correlation was found between hsp70 expression and PS concentration. On the other hand, in the glass eels sampled from the wild results showed that gene expression patterns tend to cluster based on estuary’s pollution levels. Specifically, hsp70 gene expression was upregulated in eels from the most polluted estuaries, suggesting signs of damage. This study highlights the molecular effects and signatures of emerging and legacy pollutants on the critically endangered European eel, under natural and controlled conditions.

了解全球变化的影响对保护欧洲鳗鲡（Anguilla Anguilla）等极度濒危物种至关重要。新出现的污染物（微塑料）和遗留的污染物（如重金属）以及热污染对该物种有害；然而，它们对进入河口的玻璃鳗的分子影响仍未得到充分研究。研究环境压力的分子特征可以深入了解导致安圭拉种群数量下降的一些因素。在这项研究中，我们测量了在水族箱中暴露于聚苯乙烯（PS）微珠的玻璃鳗鳃组织中与一般和热应激（hsp70和hsp90）、氧化应激（sod1、sod2、cat、gpx1）、重金属解毒（mt2）和神经毒性（ache）相关的候选基因的表达变化，以及在比斯开湾六个不同微塑料、重金属、亚硝酸盐、硝酸盐和热污染水平的河口取样的个体。在水缸实验中，hsp70的表达与PS浓度呈显著正相关。另一方面，在野外取样的玻璃鳗中，结果表明基因表达模式倾向于基于河口污染水平的聚类。具体来说，来自污染最严重的河口的鳗鱼的hsp70基因表达上调，表明有损伤的迹象。本研究强调了在自然和受控条件下，新兴和遗留污染物对极度濒危的欧洲鳗鱼的分子效应和特征。

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引用次数: 0

Bioconcentration, biotransformation, oxidative stress and neurochemical response of environmental concentration diazepam in yellowbelly pufferfish 环境浓度安定在黄腹河豚体内的生物浓度、生物转化、氧化应激及神经化学反应

IF 4.3 2区环境科学与生态学 Q1 MARINE & FRESHWATER BIOLOGY

Aquatic Toxicology

Pub Date : 2025-10-27 DOI: 10.1016/j.aquatox.2025.107620

Youyou Hu , Wenjie Hou , Yuping Zhang , Hongjie Zhu

The benzodiazepine diazepam is an emerging environmental contaminant that poses a potential threat to ecosystems and human health. However, there are knowledge gaps regarding tissue-specific accumulation and biotransformation, and the potential toxicity of diazepam in economically important fish at environmental concentrations. This study explored the tissue distribution and accumulation of diazepam and its metabolites in juvenile yellowbelly pufferfish after 35 days of exposure to environmentally relevant concentrations (2, 20, and 100 ng/L). Additionally, changes in oxidative stress markers and neurotransmitter levels were analyzed during a 35-day exposure period and a 7-day recovery period. Our results showed that after 35-day exposure, diazepam exhibited the highest accumulation in the liver tissue of yellowbelly pufferfish, followed by the brain tissue. Moreover, biotransformation occurred in vivo, including demethylation and hydroxylation. Significant differences in superoxide dismutase, catalase, and glutathione peroxidase enzyme activity between the medium and high-level treatment groups reflected the compensatory effect of antioxidant defenses, while the unchanged malondialdehyde levels indicated their strong antioxidant stress capacity. Furthermore, the down-regulation of key neurotransmitters (glutamate, γ-aminobutyric acid, dopamine, 5-hydroxytryptamine, and norepinephrine) in brain tissues during the exposure period and recovery period confirms the high potential for neurotoxicity at environmental concentrations of diazepam. These results provide fundamental support for an in-depth understanding of the tissue-specific accumulation and potential toxicity of diazepam and its metabolites to marine organisms.

苯二氮卓类地西泮是一种新兴的环境污染物，对生态系统和人类健康构成潜在威胁。然而，关于组织特异性积累和生物转化，以及地西泮在环境浓度下对经济上重要的鱼类的潜在毒性，存在知识空白。本研究探讨了地西泮及其代谢物在黄腹河豚幼鱼暴露于环境相关浓度（2、20和100 ng/L） 35天后的组织分布和积累。此外，在35天的暴露期和7天的恢复期，分析氧化应激标志物和神经递质水平的变化。我们的研究结果表明，暴露35天后，地西泮在黄腹河豚的肝脏组织中积累最多，其次是脑组织。此外，生物转化发生在体内，包括去甲基化和羟基化。中高水平处理组超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶活性差异显著，反映了抗氧化防御的代偿作用，而丙二醛水平不变，表明其抗氧化应激能力强。此外，在暴露期和恢复期，脑组织中关键神经递质（谷氨酸、γ-氨基丁酸、多巴胺、5-羟色胺和去甲肾上腺素）的下调证实了环境浓度的地西泮具有很高的神经毒性。这些结果为深入了解地西泮及其代谢物对海洋生物的组织特异性积累和潜在毒性提供了基础支持。

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引用次数: 0

Interactions between cyanobacteria and emerging contaminants in aqueous environments 蓝藻与水环境中新兴污染物之间的相互作用

IF 4.3 2区环境科学与生态学 Q1 MARINE & FRESHWATER BIOLOGY

Aquatic Toxicology

Pub Date : 2025-10-27 DOI: 10.1016/j.aquatox.2025.107621

Yichen Yang , Xiaohan Zhang , Lingshen Tan , Rui Xin , Yongzheng Ma , Zhiguang Niu

Cyanobacteria, prevalent in aquatic environments, are key contributors to the Earth's primary productivity and have historically transformed the planet's surface from anaerobic to aerobic conditions. However, due to improper disposal, a large number of emerging contaminants (ECs) were released into water bodies, which influenced the growth and physiological activities of cyanobacteria. Given the biological representativeness of cyanobacteria, their response to ECs serves as a model for understanding the impact of environmental stressors on other organisms. Thus, this review summarized the effects of ECs, including antibiotics, endocrine disrupting chemicals (EDCs), persistent organic pollutants (POPs), and microplastics (MPs) on cyanobacterial growth, morphology, and toxin production. It also explored the effect of cyanobacteria on ECs and reviewed their interactions with EC-related compounds. Additionally, the study highlighted that cyanobacteria could produce endocrine-disrupting toxins and antibiotic resistance genes (ARGs), while their secondary metabolites could also treat ECs by adsorption and settlement. Compared to the previous ones, this review innovatively focused on the bidirectional perspective. It not only examined the impact of ECs on cyanobacteria but also investigated the influence of cyanobacteria on ECs, particularly in terms of biosorption, biodegradation, and biotransformation. Consequently, this review offered a more balanced and holistic understanding of the interactions between cyanobacteria and ECs in aquatic systems, providing valuable insights into their coexistence and suggesting the potential of using cyanobacteria as an eco-friendly approach for EC remediation.

蓝藻普遍存在于水生环境中，是地球初级生产力的关键贡献者，并在历史上将地球表面从厌氧状态转变为有氧状态。然而，由于处理不当，大量的新兴污染物（ECs）被释放到水体中，影响了蓝藻的生长和生理活动。鉴于蓝藻的生物学代表性，它们对ECs的反应可以作为理解环境应激源对其他生物影响的模型。因此，本文综述了包括抗生素、内分泌干扰物（EDCs）、持久性有机污染物（POPs）和微塑料（MPs）在内的ECs对蓝藻生长、形态和毒素产生的影响。探讨了蓝藻对ECs的影响，并综述了它们与ECs相关化合物的相互作用。此外，该研究强调蓝藻可以产生内分泌干扰毒素和抗生素抗性基因（ARGs），其次级代谢物也可以通过吸附和沉降作用治疗ECs。与以往的研究相比，本文创新性地关注了双向视角。它不仅研究了ECs对蓝藻的影响，还研究了蓝藻对ECs的影响，特别是在生物吸附、生物降解和生物转化方面。因此，本综述对水生系统中蓝藻与EC之间的相互作用提供了更平衡和全面的理解，为它们的共存提供了有价值的见解，并提出了使用蓝藻作为EC修复的生态友好方法的潜力。

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引用次数: 0

Subchronic 2,6-dichloro-1,4-benzoquinone exposure induced dose-dependent cardiac functional failure via cytoskeletal remodeling in zebrafish cardiac tissue 亚慢性2,6-二氯-1,4-苯醌暴露通过斑马鱼心脏组织细胞骨骼重塑诱导剂量依赖性心功能衰竭

IF 4.3 2区环境科学与生态学 Q1 MARINE & FRESHWATER BIOLOGY

Aquatic Toxicology

Pub Date : 2025-10-27 DOI: 10.1016/j.aquatox.2025.107622

Xue Yang , Qiwei Zhang , Lihua Yang

The subchronic cardiotoxicity of 2,6-dichloro-1,4-benzoquinone (2,6-DCBQ), an unregulated disinfection byproduct with high environmental detection rates, remains poorly characterized. Using integrated multi-omics (transcriptomics, proteomics, phosphoproteomics) and histopathological analyses in zebrafish, this study systematically elucidated its dose-dependent (low-dose, 10 nM; medium-dose, 100 nM; high-dose, 1000 nM) cardiotoxicity, from adaptive remodeling to failure, over a 35-day exposure period. A reduction in atrioventricular inflow ranging from 81.4 % to 93.9 %, along with lipid droplet accumulation and Z-disc rupture, indicate a dose-dependent cardiac crisis induced by 2,6-DCBQ. Multi-omics analyses, revealed that the kinase cascade involving braf (Myhpc2_T1545), camk2a (Mybpc3_S291), and mark3b (Ttn.1_S28131) arranged dose-dependent cytoskeletal remodeling. High-dose exposure initiated an inflammation-cytoskeleton vicious cycle, wherein chemokine-driven collagen degradation exacerbated Z-disc rupture, while lipotoxic lipid droplets recruit inflammatory infiltrates, collectively escalating irreversible cardiac decompensation. These findings demonstrate that subchronic exposure to 2,6-DCBQ initiates cardiac remodeling, escalating cardiovascular susceptibility in exposed populations.

2,6-二氯-1,4-苯醌（2,6- dcbq）是一种不受监管的消毒副产物，具有很高的环境检出率，但其亚慢性心脏毒性的特征仍然很差。通过对斑马鱼进行综合多组学（转录组学、蛋白质组学、磷蛋白质组学）和组织病理学分析，本研究系统地阐明了其剂量依赖性（低剂量，10 nM；中剂量，100 nM；高剂量，1000 nM）的心脏毒性，从适应性重塑到衰竭，持续35天的暴露期。房室流减少81.4%至93.9%，同时伴有脂滴积聚和z盘破裂，表明2,6- dcbq诱导的剂量依赖性心脏危象。多组学分析显示，涉及braf （Myhpc2_T1545）、camk2a （Mybpc3_S291）和mark3b （Ttn.1_S28131）的激酶级联可进行剂量依赖性的细胞骨架重塑。高剂量暴露引发了炎症-细胞骨架的恶性循环，其中趋化因子驱动的胶原降解加剧了z盘破裂，而脂毒性脂滴招募炎症浸润，共同加剧了不可逆的心脏失代偿。这些发现表明，亚慢性暴露于2,6- dcbq会引发心脏重塑，增加暴露人群的心血管易感性。

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引用次数: 0

Morphological, histological and multi-omics analysis revealed the molecular response of female western mosquitofish (Gambusia affinis) to levonorgestrel exposure 形态学、组织学和多组学分析揭示了雌性西蚊鱼（Gambusia affinis）对左炔诺孕酮暴露的分子反应

IF 4.3 2区环境科学与生态学 Q1 MARINE & FRESHWATER BIOLOGY

Aquatic Toxicology

Pub Date : 2025-10-26 DOI: 10.1016/j.aquatox.2025.107619

Kaichun Chen , Tongfu Lu , Weijian Chen , Shengyue Lin , Dingxian Chen , Shuzheng Ye , Weiqian Liang , Sixun Li , Binhua Deng , Jian Gong , Chong Han , Qiang Li

In recent years, the use of synthetic progestogens in medicine has increased, and their presence is frequently detected in rivers. Therefore, an increasing number of researchers are beginning to focus on the impact they have on the reproduction and development of aquatic organisms. Levonorgestrel (LNG) is a synthetic progestin widely used in oral contraceptives and is also frequently detected in rivers. To assess the impact of LNG on mosquitofish (Gambusia affinis), in this study, adult female mosquitofish were exposed to LNG at concentrations of 0, 5, 50, and 500 ng/L for 36 consecutive days, followed by morphological, histological, hepatic transcriptomic and metabolomic analyses. The results indicated that exposure to LNG reduced the weight-to-length ratio of mosquitofish and simultaneously elongated their anal fins. Furthermore, at a concentration of 500 ng/L, the gonadal index and the proportion of late-stage oocytes in mosquitofish were significantly reduced, and the transcriptional expression of the genes Cyp19a1a, Cyp11a1a, Ara, and Foxl2 was markedly inhibited. Additionally, exposure to LNG led to hepatocyte degeneration and vacuolation. Transcriptomic and metabolomic analyses of the liver identified a large number of differentially expressed genes and metabolites, which were enriched in many metabolism-related pathways, such as the Insulin signaling pathway, Biosynthesis of amino acids, and Fatty acid metabolism. In summary, these results indicated that exposure to LNG could suppress ovarian development in female mosquitofish, leading to masculinization, and caused abnormalities in liver transcription and metabolism.

近年来，合成孕激素在医学上的使用有所增加，在河流中经常检测到它们的存在。因此，越来越多的研究者开始关注它们对水生生物繁殖发育的影响。左炔诺孕酮（LNG）是一种合成黄体酮，广泛用于口服避孕药，也经常在河流中检测到。为了评估LNG对食蚊鱼（Gambusia affinis）的影响，在本研究中，成年雌性食蚊鱼连续36天暴露于浓度为0、5、50和500 ng/L的LNG中，然后进行形态学、组织学、肝脏转录组学和代谢组学分析。结果表明，接触液化天然气降低了食蚊鱼的体重与长度比，同时延长了它们的肛门鳍。500 ng/L浓度显著降低了蚊鱼的性腺指数和后期卵母细胞比例，并显著抑制了Cyp19a1a、Cyp11a1a、Ara和Foxl2基因的转录表达。此外，暴露于LNG会导致肝细胞变性和空泡化。肝脏的转录组学和代谢组学分析发现了大量差异表达的基因和代谢物，这些基因和代谢物在许多代谢相关途径中富集，如胰岛素信号通路、氨基酸的生物合成和脂肪酸代谢。综上所述，这些结果表明，LNG暴露会抑制雌性蚊鱼卵巢发育，导致雄性化，并导致肝脏转录和代谢异常。

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引用次数: 0