Aquatic Toxicology最新文献_第9页

Unlocking the combined impact of microplastics and emerging contaminants on fish: A review and meta-analysis 揭示微塑料和新污染物对鱼类的综合影响：综述与荟萃分析

IF 4.1 2区环境科学与生态学 Q1 MARINE & FRESHWATER BIOLOGY

Aquatic Toxicology

Pub Date : 2024-11-25 DOI: 10.1016/j.aquatox.2024.107176

Weiming Wu , Rupeng Du , Zhuoyun Chen , Weiwen Li , Xiaomei Huang , Zhong Pan

Microplastics (MPs) possess unique adsorptive properties that render their surfaces prone to absorbing other contaminants. When interacting with these emerging contaminants, MPs may have unpredictable negative impacts on fish. Prior studies have primarily concentrated on the impact of single contaminants, while investigations into combined pollution have not received adequate attention. Therefore, research on combined pollution holds greater practical significance. The physiological indicators of fish affected by emerging contaminants and the mechanisms behind these effects are not yet fully clear. To address this issue, a meta-analysis was performed to evaluate the impact of combined pollution of MPs-containing emerging contaminants on various aspects of fish health, encompassing behavior, consumption, development, and reproduction, along with the assessment of oxidative stress and neurotoxicity of fish. The results of the meta-analysis indicated that combined pollution adversely impacted fish reproduction, development, oxidative stress, and neurotoxicity. Importantly, significant differences were observed between fish species regarding their susceptibility to function and oxidative stress. Further investigation into the mechanisms of the impact of combined pollution on fish revealed that the magnitude of this impact is closely associated with the characteristics of the MPs themselves. MPs with higher adsorption capacities tend to lead to more severe consequences, while the impact of MPs with lower adsorption capacities relies more on their toxicity. Nevertheless, a close correlation between the duration of exposure to combined pollution and the level of oxidative stress in fish was not identified. Through a systematic analysis of existing studies, this review not only explored the cumulative effects of combined pollution on fish but also highlighted the intricate nature of such pollution within aquatic ecosystems. It contributes to the growing body of knowledge on the subject and emphasizes the need for further research to unravel the complexities associated with the combined impact of MPs-containing emerging contaminants on aquatic life.

微塑料（MPs）具有独特的吸附特性，使其表面容易吸附其他污染物。当微塑料与这些新出现的污染物相互作用时，可能会对鱼类产生不可预测的负面影响。之前的研究主要集中在单一污染物的影响上，而对综合污染的调查还没有引起足够的重视。因此，对综合污染的研究具有更大的现实意义。鱼类受新出现的污染物影响的生理指标以及这些影响背后的机制尚不完全清楚。为解决这一问题，我们进行了一项荟萃分析，以评估含 MPs 新兴污染物的综合污染对鱼类健康各方面的影响，包括行为、消费、发育和繁殖，以及鱼类氧化应激和神经毒性的评估。荟萃分析的结果表明，综合污染会对鱼类的繁殖、发育、氧化应激和神经毒性产生不利影响。重要的是，不同种类的鱼类对功能和氧化应激的易感性存在明显差异。对综合污染对鱼类影响机理的进一步研究表明，这种影响的程度与多孔介质本身的特性密切相关。吸附能力较强的多孔介质往往会导致更严重的后果，而吸附能力较弱的多孔介质的影响更多地依赖于其毒性。然而，并没有发现鱼类暴露于综合污染的时间长短与氧化应激水平之间存在密切联系。通过对现有研究的系统分析，本综述不仅探讨了综合污染对鱼类的累积效应，还强调了此类污染在水生生态系统中错综复杂的性质。该综述有助于加深对这一主题的了解，并强调有必要开展进一步研究，以揭示含有 MPs 的新兴污染物对水生生物的综合影响的复杂性。

{"title":"Unlocking the combined impact of microplastics and emerging contaminants on fish: A review and meta-analysis","authors":"Weiming Wu , Rupeng Du , Zhuoyun Chen , Weiwen Li , Xiaomei Huang , Zhong Pan","doi":"10.1016/j.aquatox.2024.107176","DOIUrl":"10.1016/j.aquatox.2024.107176","url":null,"abstract":"<div><div>Microplastics (MPs) possess unique adsorptive properties that render their surfaces prone to absorbing other contaminants. When interacting with these emerging contaminants, MPs may have unpredictable negative impacts on fish. Prior studies have primarily concentrated on the impact of single contaminants, while investigations into combined pollution have not received adequate attention. Therefore, research on combined pollution holds greater practical significance. The physiological indicators of fish affected by emerging contaminants and the mechanisms behind these effects are not yet fully clear. To address this issue, a meta-analysis was performed to evaluate the impact of combined pollution of MPs-containing emerging contaminants on various aspects of fish health, encompassing behavior, consumption, development, and reproduction, along with the assessment of oxidative stress and neurotoxicity of fish. The results of the meta-analysis indicated that combined pollution adversely impacted fish reproduction, development, oxidative stress, and neurotoxicity. Importantly, significant differences were observed between fish species regarding their susceptibility to function and oxidative stress. Further investigation into the mechanisms of the impact of combined pollution on fish revealed that the magnitude of this impact is closely associated with the characteristics of the MPs themselves. MPs with higher adsorption capacities tend to lead to more severe consequences, while the impact of MPs with lower adsorption capacities relies more on their toxicity. Nevertheless, a close correlation between the duration of exposure to combined pollution and the level of oxidative stress in fish was not identified. Through a systematic analysis of existing studies, this review not only explored the cumulative effects of combined pollution on fish but also highlighted the intricate nature of such pollution within aquatic ecosystems. It contributes to the growing body of knowledge on the subject and emphasizes the need for further research to unravel the complexities associated with the combined impact of MPs-containing emerging contaminants on aquatic life.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"278 ","pages":"Article 107176"},"PeriodicalIF":4.1,"publicationDate":"2024-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142722764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Glyphosate and glyphosate-based herbicides induce Poecilia reticulata to maintain redox equilibrium during and after coexposure to iron oxide nanoparticles (y-Fe2O3) 草甘膦和草甘膦除草剂诱导网状水蛭在共暴露于氧化铁纳米颗粒（y-Fe2O3）期间和之后维持氧化还原平衡

IF 4.1 2区环境科学与生态学 Q1 MARINE & FRESHWATER BIOLOGY

Aquatic Toxicology

Pub Date : 2024-11-24 DOI: 10.1016/j.aquatox.2024.107175

João Marcos de Lima Faria , Mariana Morozesk , Iara da Costa Souza , Victória Costa da Silva , Luiz Arthur Mendes Bataus , Simone Maria Teixeira de Sabóia-Morais , Marisa Narciso Fernandes

Iron oxide nanoparticles (IONPs) are being increasingly recognized as viable materials for environmental remediation due to their capacity to adsorb contaminants such as glyphosate (GLY) on their surfaces. Nevertheless, the ecotoxicological implications of IONPs associated with GLY necessitate thorough evaluation to ascertain the safety of such remediation strategies. In this context, the present investigation was conducted to examine hepatic biomarkers pertinent to the redox system, as well as ultrastructural hepatic alterations in Poecilia reticulata, following a 21-day exposure to environmentally relevant concentrations of IONPs, iron ions (Fe), and glyphosate in its pure form (GLY) as well as a commercial glyphosate-based herbicide (GBH). After this exposure, the fish underwent a 21-day recovery in uncontaminated water. The results indicated an increase in the activity of catalase (CAT) and glutathione S-transferase (GST) and in the concentration of glutathione (GSH) in the animals subjected to IONP+GBH and IONP+GLY treatments. This biochemical response persisted for the duration of both the exposure and recovery phases. Concurrently, hepatocytes displayed mitochondria with increased electron density, augmented lipid droplet accumulation, and expanded necrotic areas within the hepatic tissue. In contrast, fish exposed solely to IONPs exhibited sustained redox homeostasis throughout the investigative timeline. These findings suggest that the coexposure toxicity of IONP+GLY and IONP+GBH is attributable to the agent adsorbed onto the IONPs and that P. reticulata could maintain an active antioxidant defense mechanism throughout the entire study period.

氧化铁纳米颗粒（IONPs）由于其表面吸附草甘膦（GLY）等污染物的能力，越来越被认为是环境修复的可行材料。然而，与GLY相关的IONPs的生态毒理学影响需要进行彻底的评估，以确定这种修复策略的安全性。在此背景下，本研究旨在检测与氧化还原系统相关的肝脏生物标志物，以及网状水蛭在暴露于环境相关浓度的离子、铁离子（Fe）、纯草甘膦（GLY）以及商业草甘膦除草剂（GBH） 21天后肝脏超微结构的变化。暴露后，这些鱼在未受污染的水中恢复了21天。结果表明，IONP+GBH和IONP+GLY处理动物过氧化氢酶（CAT）和谷胱甘肽s -转移酶（GST）活性和谷胱甘肽（GSH）浓度均升高。这种生化反应在暴露和恢复阶段都持续存在。同时，肝细胞显示线粒体电子密度增加，脂滴积聚增加，肝组织内坏死区域扩大。相比之下，在整个研究时间内，仅暴露于IONPs的鱼表现出持续的氧化还原稳态。这些结果表明，IONP+GLY和IONP+GBH的共暴露毒性可归因于IONP上吸附的物质，并且在整个研究期间，网状假丝藤可以保持活跃的抗氧化防御机制。

{"title":"Glyphosate and glyphosate-based herbicides induce Poecilia reticulata to maintain redox equilibrium during and after coexposure to iron oxide nanoparticles (y-Fe2O3)","authors":"João Marcos de Lima Faria , Mariana Morozesk , Iara da Costa Souza , Victória Costa da Silva , Luiz Arthur Mendes Bataus , Simone Maria Teixeira de Sabóia-Morais , Marisa Narciso Fernandes","doi":"10.1016/j.aquatox.2024.107175","DOIUrl":"10.1016/j.aquatox.2024.107175","url":null,"abstract":"<div><div>Iron oxide nanoparticles (IONPs) are being increasingly recognized as viable materials for environmental remediation due to their capacity to adsorb contaminants such as glyphosate (GLY) on their surfaces. Nevertheless, the ecotoxicological implications of IONPs associated with GLY necessitate thorough evaluation to ascertain the safety of such remediation strategies. In this context, the present investigation was conducted to examine hepatic biomarkers pertinent to the redox system, as well as ultrastructural hepatic alterations in <em>Poecilia reticulata</em>, following a 21-day exposure to environmentally relevant concentrations of IONPs, iron ions (Fe), and glyphosate in its pure form (GLY) as well as a commercial glyphosate-based herbicide (GBH). After this exposure, the fish underwent a 21-day recovery in uncontaminated water. The results indicated an increase in the activity of catalase (CAT) and glutathione S-transferase (GST) and in the concentration of glutathione (GSH) in the animals subjected to IONP+GBH and IONP+GLY treatments. This biochemical response persisted for the duration of both the exposure and recovery phases. Concurrently, hepatocytes displayed mitochondria with increased electron density, augmented lipid droplet accumulation, and expanded necrotic areas within the hepatic tissue. In contrast, fish exposed solely to IONPs exhibited sustained redox homeostasis throughout the investigative timeline. These findings suggest that the coexposure toxicity of IONP+GLY and IONP+GBH is attributable to the agent adsorbed onto the IONPs and that <em>P. reticulata</em> could maintain an active antioxidant defense mechanism throughout the entire study period.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"279 ","pages":"Article 107175"},"PeriodicalIF":4.1,"publicationDate":"2024-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142743683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Maternal Daphnia magna exposure to the antidepressant sertraline causes molting disorder, multi-generational reproductive and serotonergic dysfunction 母水蚤接触抗抑郁药舍曲林会导致蜕皮障碍、多代生殖和血清素能障碍

IF 4.1 2区环境科学与生态学 Q1 MARINE & FRESHWATER BIOLOGY

Aquatic Toxicology

Pub Date : 2024-11-23 DOI: 10.1016/j.aquatox.2024.107161

Chenglong Dong , Liqiang Wang , Nikolai Barulin , Juan José Alava , Shaoquan Liu , Dongmei Xiong

Sertraline, one of the most commonly used antidepressants, has exhibited a progressively escalating trend in usage over the course of the last decades years, which have been exacerbated by the COVID-19 pandemic. Here, this study assessed the transgenerational effects of sertraline on the aquatic microcrustacean Daphnia magna, a parthenogenetic model species. The parental D. magna (G0) were exposed to environmentally relevant concentrations of sertraline (0.1 and 10 μg/L) for 21 days at individual and population level, and observed exposure triggered specific increased fecundity and desynchronized molting. These alterations were partially inherited through three subsequent non-exposed generations (G1, G2, and G3), as evidenced by increased fecundity and disordered molting in G1, reduced fecundity in G2, and reduced body size of G3-offspring. The molt-related genes neverland 1 and hormone receptor 3 were significantly different to the control group simultaneously only in the exposed generation, which may well be responsible for the molting asynchrony. Vitellogenin plays an important role in reproduction, and our results indicate that its abnormal expression persists up to G3, which was highly correlated with the expression of serotonin transporter, the drug target of sertraline. This finding suggested that sertraline possesses a sustained reproductive toxicity and disrupting potential and may be associated with serotonin dysregulation caused by compensatory feedback of serotonin transporter. In combination with male birth and upregulation of doublesex and vitellogenin, sertraline was deemed to trigger a self-defense response of D. magna, known as “abandon-ship” by increasing reproductive inputs. However, no males was found in individual reproduction test in each generation, which may suggest some interaction between sertraline and population density. Our findings emphasize that the toxic effects of sertraline can be transferred to unexposed generations, even with different adverse consequences, implying that future studies need to focus on transgenerational delayed effects and the underlying mechanisms.

舍曲林是最常用的抗抑郁药物之一，在过去几十年中，其使用量呈逐步上升趋势，而 COVID-19 的流行则加剧了这一趋势。本研究评估了舍曲林对水生微型甲壳动物大型水蚤（一种孤雌生殖的模式物种）的跨代影响。亲本大型蚤（G0）暴露于环境相关浓度的舍曲林（0.1 和 10 μg/L）中 21 天，观察暴露在个体和种群水平上引发的特异性繁殖力增加和非同步蜕皮。这些改变部分遗传给随后的三个非暴露世代（G1、G2 和 G3），表现为 G1 后代繁殖力增加和蜕皮紊乱，G2 后代繁殖力降低，G3 后代体型缩小。与蜕皮相关的基因 neverland 1 和激素受体 3 只有在暴露的一代中才同时与对照组有显著差异，这很可能是造成蜕皮不同步的原因。卵黄素在生殖过程中起着重要作用，我们的研究结果表明，卵黄素的异常表达一直持续到G3，这与舍曲林的药物靶点--5-羟色胺转运体的表达高度相关。这一结果表明，舍曲林具有持续的生殖毒性和破坏潜力，可能与血清素转运体的代偿反馈引起的血清素失调有关。舍曲林与雄性个体的出生以及双倍体素和卵黄素的上调结合在一起，被认为会通过增加生殖输入引发一种被称为 "弃船 "的自卫反应。然而，在每一代的个体繁殖试验中均未发现雄性个体，这可能表明舍曲林与种群密度之间存在某种相互作用。我们的研究结果表明，舍曲林的毒性作用可以转移到未暴露的世代，甚至产生不同的不良后果，这意味着未来的研究需要关注跨代延迟效应及其内在机制。

{"title":"Maternal Daphnia magna exposure to the antidepressant sertraline causes molting disorder, multi-generational reproductive and serotonergic dysfunction","authors":"Chenglong Dong , Liqiang Wang , Nikolai Barulin , Juan José Alava , Shaoquan Liu , Dongmei Xiong","doi":"10.1016/j.aquatox.2024.107161","DOIUrl":"10.1016/j.aquatox.2024.107161","url":null,"abstract":"<div><div>Sertraline, one of the most commonly used antidepressants, has exhibited a progressively escalating trend in usage over the course of the last decades years, which have been exacerbated by the COVID-19 pandemic. Here, this study assessed the transgenerational effects of sertraline on the aquatic microcrustacean <em>Daphnia magna</em>, a parthenogenetic model species. The parental <em>D. magna</em> (G0) were exposed to environmentally relevant concentrations of sertraline (0.1 and 10 μg/L) for 21 days at individual and population level, and observed exposure triggered specific increased fecundity and desynchronized molting. These alterations were partially inherited through three subsequent non-exposed generations (G1, G2, and G3), as evidenced by increased fecundity and disordered molting in G1, reduced fecundity in G2, and reduced body size of G3-offspring. The molt-related genes <em>neverland 1</em> and <em>hormone receptor 3</em> were significantly different to the control group simultaneously only in the exposed generation, which may well be responsible for the molting asynchrony. Vitellogenin plays an important role in reproduction, and our results indicate that its abnormal expression persists up to G3, which was highly correlated with the expression of <em>serotonin transporter</em>, the drug target of sertraline. This finding suggested that sertraline possesses a sustained reproductive toxicity and disrupting potential and may be associated with serotonin dysregulation caused by compensatory feedback of <em>serotonin transporter</em>. In combination with male birth and upregulation of <em>doublesex</em> and <em>vitellogenin</em>, sertraline was deemed to trigger a self-defense response of <em>D. magna</em>, known as “abandon-ship” by increasing reproductive inputs. However, no males was found in individual reproduction test in each generation, which may suggest some interaction between sertraline and population density. Our findings emphasize that the toxic effects of sertraline can be transferred to unexposed generations, even with different adverse consequences, implying that future studies need to focus on transgenerational delayed effects and the underlying mechanisms.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"278 ","pages":"Article 107161"},"PeriodicalIF":4.1,"publicationDate":"2024-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142691188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characteristics and potential human health risks of Paralytic Shellfish Toxins identified in eight species of bivalves from South Yellow Sea Mudflat 在南黄海滩涂八种双壳贝类中发现的麻痹性贝类毒素的特征及其对人类健康的潜在风险

IF 4.1 2区环境科学与生态学 Q1 MARINE & FRESHWATER BIOLOGY

Aquatic Toxicology

Pub Date : 2024-11-23 DOI: 10.1016/j.aquatox.2024.107174

Cheng Ding , Jinling Wu , Qingyuan Guo , Jiaxuan Luan , Kai Yang , Zhaoxia Li , Xuan Li , Jianwei Yu , Feng Liang , Bairen Yang , Tianming Chen

The consumption of bivalves contaminated with paralytic shellfish toxins (PSTs) poses a serious risk to human health. However, the presence of PSTs in bivalves from the South Yellow Sea Mudflat remains unclear. This study comprehensively examined the characteristics and potential health risks of PSTs in eight species of bivalves from the South Yellow Sea Mudflat across four seasons. Typical PSTs, including STX, dcNeoSTX, GTX1, GTX2, GTX3, and GTX4, were detected in white clams, clams, short-necked clams, blue mussels, razor clams, mussels, scallops, and oysters. Significant differences of PSTs concentrations among bivalves across different seasons were detected using Kruskal-Wallis tests (p < 0.05), with the highest PSTs concentrations found in mussels (20.46 μg/individual) during autumn. Furthermore, Pearson tests revealed significant positive correlations between PSTs concentrations and shell length, shell height, shell width, and soft tissue wet weight, indicating that larger bivalves contain higher PSTs levels. The highest dietary toxin intake (DTI) of PSTs across the four seasons was found in mussels (2.138 μgSTX eq. kg⁻¹ bw day⁻¹) during autumn. Notably, the exposure risk index (ERI) from bivalve consumption for male consumers was 1.23 ± 0.819, which was higher than that for female consumers (1.102 ± 0.735). The ERI of PSTs for children aged 2–7 and the elderly over 65 were 1.448 ± 0.957 and 1.316 ± 0.874, respectively, which were higher than those for other age groups, indicating that children and the elderly are more sensitive to PSTs. It is important to note that most ERIs of PSTs from total tissues were higher than 1 (potential risk), while ERIs of PSTs from non-digestive tissues were lower than 1, suggesting that potential health risks could be reduced by removing the digestive tissues of bivalves before consumption. This study provides valuable information for mitigating health risks associated with bivalve consumption.

食用被麻痹性贝类毒素（PSTs）污染的双壳贝类会对人类健康造成严重危害。然而，南黄海泥滩双壳贝类中是否存在 PSTs 仍不清楚。这项研究全面考察了南黄海滩涂八种双壳贝类四季中 PSTs 的特征和潜在健康风险。在白蛤、文蛤、短颈蛤、蓝贻贝、蛏子、贻贝、扇贝和牡蛎中检测到了典型的 PSTs，包括 STX、dcNeoSTX、GTX1、GTX2、GTX3 和 GTX4。通过 Kruskal-Wallis 检验发现，不同季节双壳贝类的 PSTs 浓度存在显著差异（p < 0.05），其中秋季贻贝的 PSTs 浓度最高（20.46 μg/个）。此外，Pearson 检验表明，PSTs 浓度与贝壳长度、贝壳高度、贝壳宽度和软组织湿重之间存在显著的正相关关系，表明较大的双壳贝类含有较高的 PSTs 含量。在四个季节中，贻贝的 PSTs 膳食毒素摄入量（DTI）最高，为秋季（2.138 μgSTX eq. kg-¹ bw day-¹）。值得注意的是，男性消费者从双壳贝类摄入的风险指数为 1.23 ± 0.819，高于女性消费者（1.102 ± 0.735）。2 至 7 岁儿童和 65 岁以上长者的摄入量ERI 分别为 1.448 ± 0.957 和 1.316 ± 0.874，高于其他年龄组，表明儿童和长者对PST更为敏感。值得注意的是，总组织中 PST 的 ERI 值大多高于 1（潜在风险），而非消化组织中 PST 的 ERI 值则低于 1，这表明食用前去除双壳贝类的消化组织可降低潜在的健康风险。这项研究为降低与食用双壳贝类有关的健康风险提供了有价值的信息。

{"title":"Characteristics and potential human health risks of Paralytic Shellfish Toxins identified in eight species of bivalves from South Yellow Sea Mudflat","authors":"Cheng Ding , Jinling Wu , Qingyuan Guo , Jiaxuan Luan , Kai Yang , Zhaoxia Li , Xuan Li , Jianwei Yu , Feng Liang , Bairen Yang , Tianming Chen","doi":"10.1016/j.aquatox.2024.107174","DOIUrl":"10.1016/j.aquatox.2024.107174","url":null,"abstract":"<div><div>The consumption of bivalves contaminated with paralytic shellfish toxins (PSTs) poses a serious risk to human health. However, the presence of PSTs in bivalves from the South Yellow Sea Mudflat remains unclear. This study comprehensively examined the characteristics and potential health risks of PSTs in eight species of bivalves from the South Yellow Sea Mudflat across four seasons. Typical PSTs, including STX, dcNeoSTX, GTX1, GTX2, GTX3, and GTX4, were detected in white clams, clams, short-necked clams, blue mussels, razor clams, mussels, scallops, and oysters. Significant differences of PSTs concentrations among bivalves across different seasons were detected using Kruskal-Wallis tests (<em>p</em> < 0.05), with the highest PSTs concentrations found in mussels (20.46 μg/individual) during autumn. Furthermore, Pearson tests revealed significant positive correlations between PSTs concentrations and shell length, shell height, shell width, and soft tissue wet weight, indicating that larger bivalves contain higher PSTs levels. The highest dietary toxin intake (DTI) of PSTs across the four seasons was found in mussels (2.138 μgSTX eq. kg⁻¹ bw day⁻¹) during autumn. Notably, the exposure risk index (ERI) from bivalve consumption for male consumers was 1.23 ± 0.819, which was higher than that for female consumers (1.102 ± 0.735). The ERI of PSTs for children aged 2–7 and the elderly over 65 were 1.448 ± 0.957 and 1.316 ± 0.874, respectively, which were higher than those for other age groups, indicating that children and the elderly are more sensitive to PSTs. It is important to note that most ERIs of PSTs from total tissues were higher than 1 (potential risk), while ERIs of PSTs from non-digestive tissues were lower than 1, suggesting that potential health risks could be reduced by removing the digestive tissues of bivalves before consumption. This study provides valuable information for mitigating health risks associated with bivalve consumption.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"278 ","pages":"Article 107174"},"PeriodicalIF":4.1,"publicationDate":"2024-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142722766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Establishment of a cell culture from Daphnia magna as an in vitro model for (eco)toxicology assays: Case study using Bisphenol A as a representative cytotoxic and endocrine disrupting chemical 大水蚤细胞培养物作为体外（生态）毒理学分析模型的建立：双酚a作为细胞毒性和内分泌干扰化学物的代表性案例研究

IF 4.1 2区环境科学与生态学 Q1 MARINE & FRESHWATER BIOLOGY

Aquatic Toxicology

Pub Date : 2024-11-23 DOI: 10.1016/j.aquatox.2024.107173

Sreevidya CP , Manoj Kumar TM , Soumya Balakrishnan , Suresh Kunjiraman , Manomi Sarasan , Jason T. Magnuson , Jayesh Puthumana

Bisphenol A (BPA) is a widely used industrial compound found in polycarbonate plastics, epoxy resin, and various polymer materials, leading to its ubiquitous presence in the environment. The toxicity of BPA to aquatic organisms has been well documented following in vivo exposure scenarios, with known cytotoxic and endocrine-disrupting effects. As such, BPA was used in this study as a well-characterized chemical to implement more ethical and resource-efficient scientific practices in toxicity testing through new approach methods (NAMs). Due to the frequent use of Daphnia spp. as a model organism in toxicology, we developed an in vitro cell culture system from Daphnia magna embryos, with optimized medium to support cell longevity. The cultures were maintained for up to two months, demonstrating their stability and suitability for cytotoxicity studies. Using this novel system, lethal concentration 50 (LC₅₀) values were determined at the 24 and 48 h time points following BPA exposure. Subsequently, oxidative stress, endocrine disruption, and DNA damage were assessed through gene expression, activity assays, and a comet assay in BPA-exposed cells. LC₅₀ values of 52 µM and 20 µM BPA were calculated after 24 and 48 h exposures, respectively. BPA cells exposed to 20 and 52 µM had significantly increased GSH, GPx, and GST activity levels. mRNA expression analysis revealed significant upregulations in the expression of hsp70, hsp90, gst, gpx, vtg1, and cyp4, with downregulations of sod, cat, and ecr following BPA exposure. Furthermore, comet assays showed a significantly higher level of DNA damage induced by BPA compared to controls, with greater comet and tail lengths. This study established a novel in vitro Daphnia model, using BPA as a case study for determining toxic effects, further highlighting the importance and applicability of utilizing alternative methods in ecotoxicological research through reducing animal use.

双酚A （BPA）是一种广泛使用的工业化合物，存在于聚碳酸酯塑料、环氧树脂和各种聚合物材料中，导致其在环境中无处不在。BPA对水生生物的毒性在体内暴露后已被充分证明，具有已知的细胞毒性和内分泌干扰作用。因此，在本研究中，双酚a作为一种特性良好的化学物质，通过新的方法（NAMs）在毒性测试中实施更符合伦理和资源效率的科学实践。由于水蚤在毒理学研究中经常被用作模式生物，我们建立了水蚤大胚胎体外细胞培养体系，并优化了培养基以支持细胞寿命。培养物保存长达两个月，证明其稳定性和细胞毒性研究的适用性。利用这种新系统，测定了BPA暴露后24和48小时的致死浓度50 （LC50）值。随后，通过bpa暴露细胞的基因表达、活性测定和彗星测定来评估氧化应激、内分泌干扰和DNA损伤。分别计算52µM和20µM双酚a暴露24和48 h后的LC50值。暴露于20和52µM的BPA细胞显著增加GSH、GPx和GST活性水平。mRNA表达分析显示，BPA暴露后，hsp70、hsp90、gst、gpx、vtg1和cyp4的表达显著上调，sod、cat和ecr的表达下调。此外，彗星实验显示BPA引起的DNA损伤水平明显高于对照组，彗星和尾巴长度也更大。本研究建立了一种新的水蚤体外模型，以双酚a为案例研究确定毒性效应，进一步强调了通过减少动物使用来利用替代方法进行生态毒理学研究的重要性和适用性。

{"title":"Establishment of a cell culture from Daphnia magna as an in vitro model for (eco)toxicology assays: Case study using Bisphenol A as a representative cytotoxic and endocrine disrupting chemical","authors":"Sreevidya CP , Manoj Kumar TM , Soumya Balakrishnan , Suresh Kunjiraman , Manomi Sarasan , Jason T. Magnuson , Jayesh Puthumana","doi":"10.1016/j.aquatox.2024.107173","DOIUrl":"10.1016/j.aquatox.2024.107173","url":null,"abstract":"<div><div>Bisphenol A (BPA) is a widely used industrial compound found in polycarbonate plastics, epoxy resin, and various polymer materials, leading to its ubiquitous presence in the environment. The toxicity of BPA to aquatic organisms has been well documented following <em>in vivo</em> exposure scenarios, with known cytotoxic and endocrine-disrupting effects. As such, BPA was used in this study as a well-characterized chemical to implement more ethical and resource-efficient scientific practices in toxicity testing through new approach methods (NAMs). Due to the frequent use of <em>Daphnia</em> spp. as a model organism in toxicology, we developed an <em>in vitro</em> cell culture system from <em>Daphnia magna</em> embryos, with optimized medium to support cell longevity. The cultures were maintained for up to two months, demonstrating their stability and suitability for cytotoxicity studies. Using this novel system, lethal concentration 50 (LC<sub>50</sub>) values were determined at the 24 and 48 h time points following BPA exposure. Subsequently, oxidative stress, endocrine disruption, and DNA damage were assessed through gene expression, activity assays, and a comet assay in BPA-exposed cells. LC<sub>50</sub> values of 52 µM and 20 µM BPA were calculated after 24 and 48 h exposures, respectively. BPA cells exposed to 20 and 52 µM had significantly increased GSH, GPx, and GST activity levels. mRNA expression analysis revealed significant upregulations in the expression of <em>hsp70, hsp90, gst, gpx, vtg1</em>, and <em>cyp4</em>, with downregulations of <em>sod, cat</em>, and <em>ecr</em> following BPA exposure. Furthermore, comet assays showed a significantly higher level of DNA damage induced by BPA compared to controls, with greater comet and tail lengths. This study established a novel <em>in vitro Daphnia</em> model, using BPA as a case study for determining toxic effects, further highlighting the importance and applicability of utilizing alternative methods in ecotoxicological research through reducing animal use.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"278 ","pages":"Article 107173"},"PeriodicalIF":4.1,"publicationDate":"2024-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142744511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integration of physiology, microbiota and metabolomics reveals toxic response of zebrafish gut to co-exposure to polystyrene nanoplastics and arsenic 生理学、微生物群和代谢组学的整合揭示了斑马鱼肠道对同时暴露于聚苯乙烯纳米塑料和砷的毒性反应

IF 4.1 2区环境科学与生态学 Q1 MARINE & FRESHWATER BIOLOGY

Aquatic Toxicology

Pub Date : 2024-11-19 DOI: 10.1016/j.aquatox.2024.107172

Guoxin Li , Min Lv , Haitao Yu , Heng zhang , Dandan Zhang , Qingsong Li , Lei Wang , Yicheng Wu

Both nanoplastic (NP) particles and arsenic (As) are widespread in aquatic environments and pose a combined risk of exposure to aquatic organisms. How the gut of aquatic organisms responds to combined risk of exposure is still unclear. In this study, zebrafish (Danio rerio) were subjected to three distinct As stress environments: only As group (10 μg/L), and As combined with different concentrations of polystyrene (PS) NPs (1 mg/L and 10 mg/L) groups for 21 days via semi-static waterborne exposure. The physiological responses to combined stress, the diversity of gut microorganisms, and the metabolomic response of the gut were investigated. The findings indicated that PSNPs were prevalent in the intestines of zebrafish in the co-exposed group. Furthermore, the administration of 1 mg/L and 10 mg/L of PSNPs in the co-exposed group was observed to elevate As levels in the intestines by 24.88% and 76.95%, respectively, in comparison to As treatment alone. Simultaneous exposure of the gut to PSNPs and As resulted in increased contents/activities of MDA, SOD, CAT, and GST, and a decrease in contents/activities of GSH and GPx, when compared to As exposure alone. Additionally, the combined exposure led to an elevated expression of the Cu/Zn-sod, Mn-sod, gpx, and cat genes. The combined treatment with NPs and As resulted in an increase in the abundance of Proteobacteria and Fusobacteriota at the phylum level, as well as a significant increase in the abundance of Cetobacterium, Rhodococcus, and Bacteroides at the genus level. Non-targeted metabolomics analyses suggest that metabolic pathways affected by co-exposure include glycerophospholipid metabolism, glycerolipid metabolism, ABC transporters and autophagy. The findings of this study are of considerable significance for the evaluation of the toxicological impact of co-existing pollutants.

纳米塑料（NP）颗粒和砷（As）都广泛存在于水生环境中，并对水生生物构成综合暴露风险。水生生物的肠道如何应对综合暴露风险仍不清楚。在这项研究中，斑马鱼（Danio rerio）通过半静态水传播暴露于三种不同的砷胁迫环境：仅砷组（10 μg/L）和砷与不同浓度的聚苯乙烯（PS）NPs（1 mg/L和10 mg/L）组，为期21天。研究人员对综合应激的生理反应、肠道微生物的多样性以及肠道的代谢组学反应进行了调查。研究结果表明，共同暴露组斑马鱼肠道中普遍存在 PSNPs。此外，与单独施用砷处理相比，在共同暴露组中施用 1 毫克/升和 10 毫克/升的 PSNPs 可使斑马鱼肠道中的砷含量分别升高 24.88% 和 76.95%。与单独暴露于 As 相比，肠道同时暴露于 PSNPs 和 As 会导致 MDA、SOD、CAT 和 GST 的含量/活性增加，而 GSH 和 GPx 的含量/活性降低。此外，联合暴露还导致 Cu/Zn-sod、Mn-sod、gpx 和 cat 基因的表达升高。氮氧化物和砷的联合处理导致门级的变形菌和镰刀菌丰度增加，以及属级的鲸杆菌、红球菌和乳杆菌丰度显著增加。非靶向代谢组学分析表明，受共同暴露影响的代谢途径包括甘油磷脂代谢、甘油酯代谢、ABC 转运体和自噬。这项研究的结果对于评估共存污染物的毒理学影响具有重要意义。

{"title":"Integration of physiology, microbiota and metabolomics reveals toxic response of zebrafish gut to co-exposure to polystyrene nanoplastics and arsenic","authors":"Guoxin Li , Min Lv , Haitao Yu , Heng zhang , Dandan Zhang , Qingsong Li , Lei Wang , Yicheng Wu","doi":"10.1016/j.aquatox.2024.107172","DOIUrl":"10.1016/j.aquatox.2024.107172","url":null,"abstract":"<div><div>Both nanoplastic (NP) particles and arsenic (As) are widespread in aquatic environments and pose a combined risk of exposure to aquatic organisms. How the gut of aquatic organisms responds to combined risk of exposure is still unclear. In this study, zebrafish (<em>Danio rerio</em>) were subjected to three distinct As stress environments: only As group (10 μg/L), and As combined with different concentrations of polystyrene (PS) NPs (1 mg/L and 10 mg/L) groups for 21 days via semi-static waterborne exposure. The physiological responses to combined stress, the diversity of gut microorganisms, and the metabolomic response of the gut were investigated. The findings indicated that PSNPs were prevalent in the intestines of zebrafish in the co-exposed group. Furthermore, the administration of 1 mg/L and 10 mg/L of PSNPs in the co-exposed group was observed to elevate As levels in the intestines by 24.88% and 76.95%, respectively, in comparison to As treatment alone. Simultaneous exposure of the gut to PSNPs and As resulted in increased contents/activities of MDA, SOD, CAT, and GST, and a decrease in contents/activities of GSH and GPx, when compared to As exposure alone. Additionally, the combined exposure led to an elevated expression of the <em>Cu/Zn-sod, Mn-sod, gpx</em>, and <em>cat</em> genes. The combined treatment with NPs and As resulted in an increase in the abundance of Proteobacteria and Fusobacteriota at the phylum level, as well as a significant increase in the abundance of <em>Cetobacterium, Rhodococcus</em>, and <em>Bacteroides</em> at the genus level. Non-targeted metabolomics analyses suggest that metabolic pathways affected by co-exposure include glycerophospholipid metabolism, glycerolipid metabolism, ABC transporters and autophagy. The findings of this study are of considerable significance for the evaluation of the toxicological impact of co-existing pollutants.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"278 ","pages":"Article 107172"},"PeriodicalIF":4.1,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142722765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The role of chorion integrity on the bioaccumulation and toxicity of selenium nanoparticles in Japanese medaka (Oryzias latipes) 绒毛膜完整性对纳米硒粒子在日本青鳉体内的生物累积和毒性的影响

IF 4.1 2区环境科学与生态学 Q1 MARINE & FRESHWATER BIOLOGY

Aquatic Toxicology

Pub Date : 2024-11-19 DOI: 10.1016/j.aquatox.2024.107170

Hongxing Chen , Melissa Chernick , Wu Dong , Lingtian Xie , David E. Hinton

Selenium nanoparticles (nano-Se) have a wide range of biomedical and agricultural applications. However, there is little information on the potential toxicity of nano-Se once it enters the environment, particularly in fish. The first line of defense from contaminants that embryonic fish have is the chorion, but the degree to which the chorion protects the developing embryo is unknown. Japanese medaka (Oryzias latipes) embryos were exposed to nano-Se in a wide range of concentrations (0.1-400 µM). The importance of chorion integrity was evaluated by exposing embryos to 16 nm nano-Se under four degrees of dechorionation: intact, roughened, partially-dechorionated, fully-dechorionated. Then, effects of particle size on embryos and larvae were determined using four sizes of nano-Se particles (16, 25-50, 50, 100 nm). The results showed that nano-Se exposure reduced survival, development, and hatching. Nano-Se was observed to adsorb on the chorion, with the amount decreasing with increased degree of dechorionation. Toxicity increased with increasing degree of dechorionation, and smaller-sized nano-Se crossed intact chorion more readily and resulted in higher toxicity than larger ones. In larvae, nano-Se accumulated on the skin and was more toxic compared to embryos. This study demonstrated the importance of the chorion in protecting developing embryos and effects of nanoparticle size on its bioavailability and subsequent toxicity.

纳米硒（nano-Se）具有广泛的生物医学和农业用途。然而，纳米硒一旦进入环境，尤其是鱼类体内，其潜在毒性方面的信息却很少。胚胎鱼类抵御污染物的第一道防线是绒毛膜，但绒毛膜对发育中胚胎的保护程度尚不清楚。日本青鳉（Oryzias latipes）胚胎暴露于各种浓度（0.1-400 µM）的纳米硒。通过将胚胎暴露在 16 纳米纳米硒中，评估了绒毛膜完整性的重要性，绒毛膜完整性包括四种程度的去绒毛膜化：完整、粗糙、部分去绒毛膜化和完全去绒毛膜化。然后，使用四种尺寸的纳米硒颗粒（16、25-50、50、100 nm）测定了颗粒尺寸对胚胎和幼虫的影响。结果表明，纳米硒暴露会降低胚胎和幼虫的存活、发育和孵化率。纳米硒被观察到吸附在绒毛膜上，其数量随着脱绒程度的增加而减少。毒性随绒毛膜脱落程度的增加而增加，尺寸较小的纳米硒比尺寸较大的纳米硒更容易穿过完整的绒毛膜，导致毒性增加。在幼虫体内，纳米硒积聚在皮肤上，与胚胎相比毒性更大。这项研究证明了绒毛膜在保护发育中胚胎方面的重要性，以及纳米粒子的大小对其生物利用率和后续毒性的影响。

{"title":"The role of chorion integrity on the bioaccumulation and toxicity of selenium nanoparticles in Japanese medaka (Oryzias latipes)","authors":"Hongxing Chen , Melissa Chernick , Wu Dong , Lingtian Xie , David E. Hinton","doi":"10.1016/j.aquatox.2024.107170","DOIUrl":"10.1016/j.aquatox.2024.107170","url":null,"abstract":"<div><div>Selenium nanoparticles (nano-Se) have a wide range of biomedical and agricultural applications. However, there is little information on the potential toxicity of nano-Se once it enters the environment, particularly in fish. The first line of defense from contaminants that embryonic fish have is the chorion, but the degree to which the chorion protects the developing embryo is unknown. Japanese medaka (<em>Oryzias latipes</em>) embryos were exposed to nano-Se in a wide range of concentrations (0.1-400 µM). The importance of chorion integrity was evaluated by exposing embryos to 16 nm nano-Se under four degrees of dechorionation: intact, roughened, partially-dechorionated, fully-dechorionated. Then, effects of particle size on embryos and larvae were determined using four sizes of nano-Se particles (16, 25-50, 50, 100 nm). The results showed that nano-Se exposure reduced survival, development, and hatching. Nano-Se was observed to adsorb on the chorion, with the amount decreasing with increased degree of dechorionation. Toxicity increased with increasing degree of dechorionation, and smaller-sized nano-Se crossed intact chorion more readily and resulted in higher toxicity than larger ones. In larvae, nano-Se accumulated on the skin and was more toxic compared to embryos. This study demonstrated the importance of the chorion in protecting developing embryos and effects of nanoparticle size on its bioavailability and subsequent toxicity.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"278 ","pages":"Article 107170"},"PeriodicalIF":4.1,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142691187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Derivation of toxicity parameters from field data: Analysis of lake zooplankton species responses to metals and acidity 野外数据中毒性参数的推导：湖泊浮游动物对金属和酸度的反应分析

IF 4.1 2区环境科学与生态学 Q1 MARINE & FRESHWATER BIOLOGY

Aquatic Toxicology

Pub Date : 2024-11-19 DOI: 10.1016/j.aquatox.2024.107169

E. Tipping , W. Keller , B.A. Edwards , S. Lofts

The WHAM-F_TOXβ model describes the toxic effects of mixtures of protons and metal cations towards biological species, using a set of intrinsic parameters for the cations (α_H, α_M*) and a sensitivity parameter (β) for each species. We applied the model to extensive water chemistry and zooplankton species occurrence data for four lakes contaminated with acidity and metals (Al, Ni, Cu, Zn) at Sudbury, Ontario, over the period 1973-2018, during which cation contamination declined, and zooplankton species numbers increased. Assuming that the appearance of a species resulted solely from decreases in water toxicity, and that α_H and α_M* values previously derived from laboratory toxicity test data could be applied in the field, we used the field data to estimate values of β for individual lake zooplankton species. Results for lake-species pairs with 20 or more species occurrences (from six samplings per year) were analysed. In most cases, the number of occurrences increased over time from zero to five or six per year, then remained at the high level. For a minority of pairs, occurrences per year increased initially, but subsequently declined, and so data only from the initial period were used to estimate β. The β values derived for the lake zooplankton are reasonably consistent with values derived from laboratory data for a range of other species. The findings support the application of WHAM-F_TOXβ to describe toxic effects of mixtures of cations in the field, and the toxicity model might be combined with ecological theory to interpret natural population responses.

WHAM-FTOXβ模型描述了质子和金属阳离子混合物对生物物种的毒性作用，使用了一组阳离子的本征参数（αH, αM*）和每个物种的灵敏度参数（β）。我们将该模型应用于1973年至2018年期间安大略省萨德伯里四个被酸性和金属（Al, Ni, Cu, Zn）污染的湖泊的广泛水化学和浮游动物物种发生数据，在此期间阳离子污染下降，浮游动物物种数量增加。假设一种浮游动物的出现完全是由于水毒性的降低，并且αH和αM*值可以应用于现场，我们使用现场数据来估计单个湖泊浮游动物的β值。分析了每年6次采样中出现20种或更多物种的湖泊物种对的结果。在大多数情况下，发生的次数随着时间的推移从零增加到每年五或六次，然后保持在高水平。对于少数对，每年的发生率最初增加，但随后下降，因此仅使用最初时期的数据来估计β。湖泊浮游动物的β值与一系列其他物种的实验室数据的值相当一致。研究结果支持WHAM-FTOXβ在野外描述阳离子混合物毒性效应的应用，并可将毒性模型与生态学理论相结合来解释自然种群反应。

{"title":"Derivation of toxicity parameters from field data: Analysis of lake zooplankton species responses to metals and acidity","authors":"E. Tipping , W. Keller , B.A. Edwards , S. Lofts","doi":"10.1016/j.aquatox.2024.107169","DOIUrl":"10.1016/j.aquatox.2024.107169","url":null,"abstract":"<div><div>The WHAM-<em>F</em><sub>TOX</sub><em>β</em> model describes the toxic effects of mixtures of protons and metal cations towards biological species, using a set of intrinsic parameters for the cations (<em>α</em><sub>H</sub>, <em>α</em><sub>M</sub>*) and a sensitivity parameter (<em>β</em>) for each species. We applied the model to extensive water chemistry and zooplankton species occurrence data for four lakes contaminated with acidity and metals (Al, Ni, Cu, Zn) at Sudbury, Ontario, over the period 1973-2018, during which cation contamination declined, and zooplankton species numbers increased. Assuming that the appearance of a species resulted solely from decreases in water toxicity, and that <em>α</em><sub>H</sub> and <em>α</em><sub>M</sub>* values previously derived from laboratory toxicity test data could be applied in the field, we used the field data to estimate values of <em>β</em> for individual lake zooplankton species. Results for lake-species pairs with 20 or more species occurrences (from six samplings per year) were analysed. In most cases, the number of occurrences increased over time from zero to five or six per year, then remained at the high level. For a minority of pairs, occurrences per year increased initially, but subsequently declined, and so data only from the initial period were used to estimate <em>β</em>. The <em>β</em> values derived for the lake zooplankton are reasonably consistent with values derived from laboratory data for a range of other species. The findings support the application of WHAM-<em>F</em><sub>TOX</sub><em>β</em> to describe toxic effects of mixtures of cations in the field, and the toxicity model might be combined with ecological theory to interpret natural population responses.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"279 ","pages":"Article 107169"},"PeriodicalIF":4.1,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142756109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of perfluoroalkyl acids on nitrogen release, transformation and microbial community during the debris decomposition of Alisma orientale and Iris pseudacorus 全氟烷基酸对东方鸢尾和假鸢尾碎屑分解过程中氮释放、转化和微生物群落的影响

IF 4.1 2区环境科学与生态学 Q1 MARINE & FRESHWATER BIOLOGY

Aquatic Toxicology

Pub Date : 2024-11-19 DOI: 10.1016/j.aquatox.2024.107171

Xiaoqing Li , Zulin Hua , Jianyun Zhang , Junliang Jin , Yifan Wang

The release of nutrients into water during debris decomposition is a serious concern, leading to severe environmental pollution. To understand the effects of extensively present emerging contaminants (such as perfluoroalkyl acids (PFAAs)) on the nitrogen (N) release and transformation, the concentration dynamics of different N species in surrounding water and changes in microbial communities on biofilm during the 70-days decomposition of two typical submerged macrophyte (Alisma orientale and Iris pseudacorus) debris were studied. The results showed that large amounts of N species (especially organic and ammonium N) were released during decomposition. PFAAs with a low concentration (1 μg/L) could stimulate total N (TN) release, whereas PFAAs with a high concentration (≥ 10 μg/L) might have inhibited TN release. Higher intensities of ammonification, nitrosification, and denitrification, but lower intensities of nitrification were observed in water in the presence of PFAAs. Microbiota associated with organic matter hydrolysis, nitrification and denitrification, as well as PFAA degrading/tolerant bacteria, were beneficial and might have occupied dominant states. Redundancy analysis showed that PFAAs were positively associated with the amounts of nitrate, denitrifiers, and azotobacteria but negatively correlated with the TN, ammonia, nitrite, organic N, and nitrosobacteria amounts (p = 0.0002). The complete N metabolism pathway was identified using PICRUSt and KEGG. Functional genes related to ammonification (0.76‰–2.16‰), N reduction (3.43‰–5.05‰), and assimilation (0.81‰–2.16‰) were more abundant than others in all treatments. This study provides a more comprehensive understanding of N cycling during debris decomposition under the increasingly intractable threat of emerging contaminants in aquatic ecosystems.

碎片分解过程中向水中释放营养物质是一个令人严重关切的问题，会导致严重的环境污染。为了了解广泛存在的新污染物（如全氟烷基酸（PFAAs））对氮（N）释放和转化的影响，研究了两种典型水下大型植物（东方鸢尾和假鸢尾）残片在 70 天分解过程中，周围水体中不同氮物种的浓度动态以及生物膜上微生物群落的变化。结果表明，分解过程中释放出大量氮（尤其是有机氮和铵态氮）。低浓度（1 μg/L）的全氟辛烷磺酸可刺激总氮（TN）的释放，而高浓度（≥ 10 μg/L）的全氟辛烷磺酸可能会抑制 TN 的释放。在含有全氟辛烷磺酸的水中，氨化、亚硝化和反硝化的强度较高，但硝化的强度较低。与有机物水解、硝化和反硝化相关的微生物群以及全氟辛烷磺酸降解菌/耐受菌对水体有益，并可能占据主导地位。冗余分析表明，全氟辛烷磺酸与硝酸盐、反硝化菌和氮细菌的数量呈正相关，但与 TN、氨氮、亚硝酸盐、有机氮和亚硝基细菌的数量呈负相关（p = 0.0002）。利用 PICRUSt 和 KEGG 确定了完整的氮代谢途径。在所有处理中，与氨化（0.76‰-2.16‰）、氮还原（3.43‰-5.05‰）和同化（0.81‰-2.16‰）相关的功能基因都比其他功能基因丰富。在水生生态系统中新出现的污染物的威胁日益严重的情况下，这项研究为人们更全面地了解碎屑分解过程中的氮循环提供了依据。

{"title":"Effects of perfluoroalkyl acids on nitrogen release, transformation and microbial community during the debris decomposition of Alisma orientale and Iris pseudacorus","authors":"Xiaoqing Li , Zulin Hua , Jianyun Zhang , Junliang Jin , Yifan Wang","doi":"10.1016/j.aquatox.2024.107171","DOIUrl":"10.1016/j.aquatox.2024.107171","url":null,"abstract":"<div><div>The release of nutrients into water during debris decomposition is a serious concern, leading to severe environmental pollution. To understand the effects of extensively present emerging contaminants (such as perfluoroalkyl acids (PFAAs)) on the nitrogen (N) release and transformation, the concentration dynamics of different N species in surrounding water and changes in microbial communities on biofilm during the 70-days decomposition of two typical submerged macrophyte (<em>Alisma orientale</em> and <em>Iris pseudacorus</em>) debris were studied. The results showed that large amounts of N species (especially organic and ammonium N) were released during decomposition. PFAAs with a low concentration (1 μg/L) could stimulate total N (TN) release, whereas PFAAs with a high concentration (≥ 10 μg/L) might have inhibited TN release. Higher intensities of ammonification, nitrosification, and denitrification, but lower intensities of nitrification were observed in water in the presence of PFAAs. Microbiota associated with organic matter hydrolysis, nitrification and denitrification, as well as PFAA degrading/tolerant bacteria, were beneficial and might have occupied dominant states. Redundancy analysis showed that PFAAs were positively associated with the amounts of nitrate, denitrifiers, and azotobacteria but negatively correlated with the TN, ammonia, nitrite, organic N, and nitrosobacteria amounts (<em>p</em> = 0.0002). The complete N metabolism pathway was identified using PICRUSt and KEGG. Functional genes related to ammonification (0.76‰–2.16‰), N reduction (3.43‰–5.05‰), and assimilation (0.81‰–2.16‰) were more abundant than others in all treatments. This study provides a more comprehensive understanding of N cycling during debris decomposition under the increasingly intractable threat of emerging contaminants in aquatic ecosystems.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"278 ","pages":"Article 107171"},"PeriodicalIF":4.1,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142691185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impacts of nanopolystyrene and/or phoxim exposure at environmentally relevant concentrations on the intestinal histopathology, intestinal microbiota, and metabolome in Eriocheir sinensis 暴露于环境相关浓度的纳米多苯乙烯和/或辛硫磷对中华鳖肠道组织病理学、肠道微生物群和代谢组的影响

IF 4.1 2区环境科学与生态学 Q1 MARINE & FRESHWATER BIOLOGY

Aquatic Toxicology

Pub Date : 2024-11-18 DOI: 10.1016/j.aquatox.2024.107168

Shuquan Ding , Shunli Che , Mengting Huang , Yuan Ma , Longteng Shen , Jianbin Feng , Xilei Li

Nanopolystyrene (NP) pollution in aquatic environments has become an increasing concern. Phoxim (PHO), one of the major organophosphorus pesticides, has also been detected in aquatic environments, posing serious health risks to crustaceans. This study aimed to assess the detrimental effects of NP and/or PHO exposure at environmentally relevant concentrations on the intestinal histopathology, intestinal microbiota, and metabolome of adult crabs (Eriocheir sinensis) for 21 days. Our study revealed significant histopathological abnormalities in the intestines. In all the exposure groups, there was a discovery of vacuolar degeneration occurring in epithelial cells. Additionally, the peritrophic membrane exhibited thinning after NP or PHO single exposure, while thickening was observed after co-exposure. Exposure to NP and/or PHO disrupted the intestinal microbiota homeostasis, as evidenced by the proliferation of pathogenic bacteria and suppression of beneficial bacteria. Notably, PHO exposure resulted in increased abundance of pathogenic bacteria (Spiroplasma and Arcobacter) and decreased abundance of beneficial bacteria (Bacteroides). Analysis of the metabolome revealed that exposure to NP and/or PHO led to alterations in the metabolic profile as well as several critical pathways. Among these, the upregulation of arachidonic acid metabolism, ABC transporters, and biosynthesis of amino acids was observed in both NP single exposure and co-exposure, while PHO single exposure downregulated these pathways. Additionally, NP and/or PHO exposure downregulated neuroactive ligand-receptor interaction. Spearman correlation analysis revealed that the significant reduction of some differentially expressed metabolites (DEMs) was potentially regulated by the low-abundance bacterial genera following exposure to NP and/or PHO. And these DEMs have a role in anti-inflammatory or antioxidant properties. Collectively, our results offer novel perspectives on the intestinal toxicity of crustaceans by NP and/or PHO at environmentally relevant concentrations.

水生环境中的纳米多苯乙烯（NP）污染日益受到关注。水生环境中也检测到了主要有机磷农药之一的辛硫磷（PHO），对甲壳类动物的健康构成严重威胁。本研究旨在评估暴露于环境相关浓度的 NP 和/或 PHO 21 天对中华绒螯蟹成蟹肠道组织病理学、肠道微生物群和代谢组的有害影响。我们的研究发现，螃蟹的肠道组织病理学出现了明显的异常。在所有暴露组中，都发现上皮细胞发生空泡变性。此外，单一暴露于 NP 或 PHO 后，营养周膜变薄，而共同暴露后，营养周膜增厚。暴露于 NP 和/或 PHO 会破坏肠道微生物群的平衡，表现为致病菌的增殖和有益菌的抑制。值得注意的是，暴露于 PHO 会导致致病菌（螺原体和弧菌）的数量增加和有益菌（乳杆菌）的数量减少。对代谢组的分析表明，暴露于 NP 和/或 PHO 会导致代谢概况以及几种关键途径的改变。其中，花生四烯酸代谢、ABC 转运体和氨基酸的生物合成在 NP 单一暴露和共同暴露中都出现了上调，而 PHO 单一暴露则下调了这些途径。此外，NP 和/或 PHO 暴露会降低神经活性配体与受体之间的相互作用。斯皮尔曼相关分析表明，暴露于 NP 和/或 PHO 后，一些差异表达代谢物（DEMs）的显著减少可能受低丰度细菌属的调控。而这些 DEMs 具有抗炎或抗氧化作用。总之，我们的研究结果为研究环境相关浓度的 NP 和/或 PHO 对甲壳动物肠道的毒性提供了新的视角。

{"title":"Impacts of nanopolystyrene and/or phoxim exposure at environmentally relevant concentrations on the intestinal histopathology, intestinal microbiota, and metabolome in Eriocheir sinensis","authors":"Shuquan Ding , Shunli Che , Mengting Huang , Yuan Ma , Longteng Shen , Jianbin Feng , Xilei Li","doi":"10.1016/j.aquatox.2024.107168","DOIUrl":"10.1016/j.aquatox.2024.107168","url":null,"abstract":"<div><div>Nanopolystyrene (NP) pollution in aquatic environments has become an increasing concern. Phoxim (PHO), one of the major organophosphorus pesticides, has also been detected in aquatic environments, posing serious health risks to crustaceans. This study aimed to assess the detrimental effects of NP and/or PHO exposure at environmentally relevant concentrations on the intestinal histopathology, intestinal microbiota, and metabolome of adult crabs (<em>Eriocheir sinensis</em>) for 21 days. Our study revealed significant histopathological abnormalities in the intestines. In all the exposure groups, there was a discovery of vacuolar degeneration occurring in epithelial cells. Additionally, the peritrophic membrane exhibited thinning after NP or PHO single exposure, while thickening was observed after co-exposure. Exposure to NP and/or PHO disrupted the intestinal microbiota homeostasis, as evidenced by the proliferation of pathogenic bacteria and suppression of beneficial bacteria. Notably, PHO exposure resulted in increased abundance of pathogenic bacteria (<em>Spiroplasma</em> and <em>Arcobacter</em>) and decreased abundance of beneficial bacteria (<em>Bacteroides</em>). Analysis of the metabolome revealed that exposure to NP and/or PHO led to alterations in the metabolic profile as well as several critical pathways. Among these, the upregulation of arachidonic acid metabolism, ABC transporters, and biosynthesis of amino acids was observed in both NP single exposure and co-exposure, while PHO single exposure downregulated these pathways. Additionally, NP and/or PHO exposure downregulated neuroactive ligand-receptor interaction. Spearman correlation analysis revealed that the significant reduction of some differentially expressed metabolites (DEMs) was potentially regulated by the low-abundance bacterial genera following exposure to NP and/or PHO. And these DEMs have a role in anti-inflammatory or antioxidant properties. Collectively, our results offer novel perspectives on the intestinal toxicity of crustaceans by NP and/or PHO at environmentally relevant concentrations.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"278 ","pages":"Article 107168"},"PeriodicalIF":4.1,"publicationDate":"2024-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142691149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0