The contamination of soil with antimony (Sb) presents a significant risk to ecological systems and public health. Conventional biochar offers limited mitigation of Sb toxicity for plants grown in highly contaminated soil. Ball milling can enhance the physicochemical properties of biochar; however, the effects of ball-milled biochar on biological interactions in the rhizosphere remain unknown. This study aimed to evaluate the effects of ball-milled biochar on the rhizosphere microenvironment, Sb accumulation, and maize growth in soil from an abandoned Sb smelting site. Three pot treatments were set up, which were soil alone (CK), soil amended with non-ball-milled biochar (SBC) and ball-milled biochar (SQBC). Each treatment was sown with maize seedlings. The results revealed that the reduction of Sb accumulation in maize accounted for 95% and 66% in SQBC treatment relative to CK and SBC treatments (P < 0.001). This immobilization was achieved through a profound shift in Sb speciation that significantly increased the stable residual fraction. Maize biomass was significantly higher in SQBC than in its counterparts (P < 0.001). Ball-milled biochar enriched beneficial bacterial taxa such as Pseudomonadota and Bacillota and drove a restructuring of the rhizosphere metabolome in Sb-polluted soils. Key detoxification pathways, including steroid hormone biosynthesis, alpha-linolenic acid metabolism, and alkaloid biosynthesis, were significantly upregulated in SQBC. This study suggests that amendment of Sb polluted soil with ball-milled biochar can create a rhizosphere environment that selectively enriches a beneficial microbiome and stimulates a detoxifying metabolome, thereby reducing Sb pollution and enhancing plant growth.
扫码关注我们
求助内容:
应助结果提醒方式:
