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Ball-milled biochar stabilizes antimony in contaminated soils by driving rhizosphere metabolite-bacteria synergy 球磨生物炭通过驱动根际代谢物-细菌协同作用稳定污染土壤中的锑
IF 5.8 2区 生物学 Q1 AGRICULTURAL ENGINEERING Pub Date : 2026-01-13 DOI: 10.1016/j.biombioe.2026.108946
Gratien Twagirayezu , Hongguang Cheng , Antong Xia , Zhibing Wu , Mohamed Abo-Eldahab , Dan Xing , Deng Linbo , Yanyou Wu
The contamination of soil with antimony (Sb) presents a significant risk to ecological systems and public health. Conventional biochar offers limited mitigation of Sb toxicity for plants grown in highly contaminated soil. Ball milling can enhance the physicochemical properties of biochar; however, the effects of ball-milled biochar on biological interactions in the rhizosphere remain unknown. This study aimed to evaluate the effects of ball-milled biochar on the rhizosphere microenvironment, Sb accumulation, and maize growth in soil from an abandoned Sb smelting site. Three pot treatments were set up, which were soil alone (CK), soil amended with non-ball-milled biochar (SBC) and ball-milled biochar (SQBC). Each treatment was sown with maize seedlings. The results revealed that the reduction of Sb accumulation in maize accounted for 95% and 66% in SQBC treatment relative to CK and SBC treatments (P < 0.001). This immobilization was achieved through a profound shift in Sb speciation that significantly increased the stable residual fraction. Maize biomass was significantly higher in SQBC than in its counterparts (P < 0.001). Ball-milled biochar enriched beneficial bacterial taxa such as Pseudomonadota and Bacillota and drove a restructuring of the rhizosphere metabolome in Sb-polluted soils. Key detoxification pathways, including steroid hormone biosynthesis, alpha-linolenic acid metabolism, and alkaloid biosynthesis, were significantly upregulated in SQBC. This study suggests that amendment of Sb polluted soil with ball-milled biochar can create a rhizosphere environment that selectively enriches a beneficial microbiome and stimulates a detoxifying metabolome, thereby reducing Sb pollution and enhancing plant growth.
土壤锑污染对生态系统和公众健康构成重大威胁。传统的生物炭对在高污染土壤中生长的植物减轻锑毒性的作用有限。球磨可提高生物炭的理化性能;然而,球磨生物炭对根际生物相互作用的影响尚不清楚。本研究旨在评价球磨生物炭对废弃锑冶炼场地土壤根际微环境、锑积累和玉米生长的影响。设置了3种盆栽处理,分别为土壤单独处理(CK)、土壤非球磨生物炭(SBC)和球磨生物炭(SQBC)。每个处理都播种玉米幼苗。结果表明,与对照和SBC处理相比,SQBC处理降低了玉米Sb积累量的95%和66% (P < 0.001)。这种固定化是通过Sb物种形成的深刻转变实现的,该转变显著增加了稳定的残余组分。玉米生物量在SQBC中显著高于对照(P < 0.001)。球磨生物炭丰富了有益细菌分类群,如假单胞菌和芽孢杆菌,并推动了硒污染土壤根际代谢组的重组。关键的解毒途径,包括类固醇激素的生物合成、α -亚麻酸代谢和生物碱的生物合成,在SQBC中显著上调。本研究表明,用球磨生物炭改良锑污染土壤可以创造一个选择性丰富有益微生物群和刺激解毒代谢组的根际环境,从而减少锑污染,促进植物生长。
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引用次数: 0
Sustainable valorization of coffee pulp: Evaluation of the pretreatment effect on the phenolic compounds recovery and anaerobic digestion of the residual biomass 咖啡浆的可持续增值:预处理对酚类化合物回收和残余生物质厌氧消化效果的评价
IF 5.8 2区 生物学 Q1 AGRICULTURAL ENGINEERING Pub Date : 2026-01-13 DOI: 10.1016/j.biombioe.2026.108969
Abdulfetah Sherefa Arega , Konstantina Tsigkou , Shimelis Kebede Kassahun , Irini Angelidaki
Coffee pulp is the main solid waste derived from wet processing, posing environmental risks if not properly handled. However, its valorization can benefit both the environment and the regional economy. This study examines the holistic approach of phenolic compounds extraction, coupled with the further valorization of the residual biomass through anaerobic digestion, while an economic assessment indicates the most favored pathway. Several pretreatment methods were considered (squeezing, grinding, ultrasounds (US), microwaves, and supercritical CO2 (SC CO2)), followed by the evaluation of the extract content in total phenolics and flavonoids, while the anaerobic digestion process for CH4 production followed. The US and SC CO2 methods showed maximum phenolics extraction efficiency up to 58.3 % and 35.2 % respectively, compared to the reference. The biochemical methane potential tests also indicated higher methane yields for the cases of SC CO2 (up to 31 %) and US (up to 22 %), although the rest of the pretreatment methods were not very promising. Finally, an economic assessment was conducted using the zero net benefit approach to investigate the minimum phenolic mixture selling price and the anaerobic digestion contribution on the profitability. Accordingly, the US pretreatment method was found to be the most efficient, with mixed phenolics selling price of 15.3 €/kg, namely 66 % less than a typical selling price, marking the US as the most economically feasible option. Overall, the study showed the importance of combining pretreatment and phenolic compounds extraction along with anaerobic digestion of the residual biomass for a sustainable valorization of coffee pulp to improve the environment and economic feasibility.
咖啡浆是湿法加工过程中产生的主要固体废物,如果处理不当,会造成环境风险。然而,它的增值对环境和区域经济都有好处。本研究考察了酚类化合物提取的整体方法,以及通过厌氧消化对残余生物质的进一步增值,而经济评估表明了最受欢迎的途径。研究了几种预处理方法(挤压、研磨、超声(US)、微波和超临界CO2 (SC CO2)),然后评估了提取物中总酚类物质和总黄酮的含量,然后进行了厌氧消化生产CH4的工艺。与参考方法相比,美国和SC CO2方法的酚类提取效率分别达到58.3%和35.2%。生化甲烷潜力测试还表明,SC CO2(高达31%)和US(高达22%)的甲烷产量更高,尽管其他预处理方法的前景不太乐观。最后,采用零净效益法进行了经济评估,考察了酚类混合物的最低销售价格和厌氧消化对盈利能力的贡献。因此,美国预处理方法被认为是最有效的,混合酚类化合物的销售价格为15.3欧元/公斤,比典型的销售价格低66%,这标志着美国是最经济可行的选择。综上所述,该研究表明,将预处理和酚类化合物提取结合起来,并对剩余生物质进行厌氧消化,对于咖啡浆的可持续增值具有重要意义,可以改善环境和经济可行性。
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引用次数: 0
RSM-ANN sequential optimization of biomass, lipid yield, and biodiesel quality from Chlorella salina 盐渍小球藻生物量、脂质产率和生物柴油质量的RSM-ANN序次优化
IF 5.8 2区 生物学 Q1 AGRICULTURAL ENGINEERING Pub Date : 2026-01-13 DOI: 10.1016/j.biombioe.2026.108977
Mohamed A. Hassaan , Nesma I.M. Abdelaziz , Murat Yılmaz , Ahmed M. Ibrahim , Mohammed S. Hassouna , Ahmed El Nemr
The urgent demand for sustainable biofuels that do not compromise food security has intensified efforts to optimize Chlorella salina (C. salina) for biomass and lipid production. This study employed a sequential experimental-modelling approach, utilizing Response Surface Methodology (RSM) in conjunction with Artificial Neural Network (ANN) validation, to optimize growth parameters that influence biomass, lipid productivity, and biodiesel attributes. When cultivated in F/2 medium, C. salina entered the stationary phase on day 9, achieving a maximum cell density of 5.39 × 106 cells/mL after 12 days, with a peak biomass yield of 312 mg L−1. The highest lipid content (26.11 %) and productivity (6.79 mg L−1 d−1) were recorded under the same conditions after 12 days. Gas chromatography revealed saturated fatty acids (SFA) at 44.04 %, while Basal SAG medium yielded maximum monounsaturated (MUFA, 36.73 %) and polyunsaturated fatty acids (PUFA, 18.23 %). RSM models exhibited excellent predictive power (R2 > 0.97), corroborated by the ANN, which showed strong alignment with the experimental data. Multi-response optimization via desirability function identified optimal conditions: 27 days of cultivation, N2 at 800 ppm, NaHCO3 at 100 ppm, and CO2 at 13 ppm. This validated sequential strategy provides a robust framework for optimizing complex biological systems, enhancing the economic feasibility of algal biofuels.
对不危及粮食安全的可持续生物燃料的迫切需求,加大了对盐小球藻(C. salina)生物质和脂质生产的优化努力。本研究采用顺序实验建模方法,利用响应面法(RSM)结合人工神经网络(ANN)验证,优化影响生物量、脂质生产力和生物柴油属性的生长参数。在F/2培养基中培养时,第9天盐藻进入固定期,12天后细胞密度达到5.39 × 106个/mL,峰值生物量为312 mg L−1。在相同条件下,12天后油脂含量最高(26.11%),产率最高(6.79 mg L−1 d−1)。气相色谱分析显示饱和脂肪酸(SFA)含量为44.04%,而基础SAG培养基中单不饱和脂肪酸(MUFA)和多不饱和脂肪酸(PUFA)含量最高,分别为36.73%和18.23%。RSM模型表现出优异的预测能力(R2 > 0.97),这一点得到了人工神经网络的证实,与实验数据有很强的一致性。通过期望函数进行多响应优化,确定了最佳条件:培养27天,N2浓度为800 ppm, NaHCO3浓度为100 ppm, CO2浓度为13 ppm。这种经过验证的顺序策略为优化复杂的生物系统提供了一个强大的框架,提高了藻类生物燃料的经济可行性。
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引用次数: 0
Unveiling the mechanisms of integrated pretreatment of ultrasonication and ascorbic acid for methane production from waste activated sludge 揭示了超声与抗坏血酸联合预处理废活性污泥产甲烷的机理
IF 5.8 2区 生物学 Q1 AGRICULTURAL ENGINEERING Pub Date : 2026-01-13 DOI: 10.1016/j.biombioe.2026.108937
Akshaya K , Rangabhashiyam Selvasembian
Anaerobic digestion is widely used for stabilizing waste activated sludge and recovering methane, but its efficiency is limited by the slow hydrolysis of complex organic matter. This study investigates a novel combined pretreatment approach using ascorbic acid (ASA) and ultrasonication (US) to enhance sludge solubilization and methane yield. The effectiveness of ASA, US, and their integration (ASA–US) was evaluated in terms of solubilized COD, biopolymer release, methane production, and microbial community shifts. The optimal combined pretreatment (0.04 g/g TSS ASA and 1.625 W/mL US) achieved 21.7 % COD solubilization—higher than ASA (7.6 %) and US (14.2 %) alone. Methane yield increased by 109.6 % compared to the control, reaching 285 ± 4 mL/g VS for ASA-US pretreated sludge. Kinetic modelling (Modified Gompertz and Logistic models) confirmed the enhanced biodegradability of the pretreated sludge. Microbial analysis revealed a notable enrichment of hydrolytic and acidogenic taxa such as Firmicutes and Clostridia, with a concurrent reduction in Proteobacteria, indicating a community shift favouring methanogenesis. Mechanistically, ASA promoted EPS disruption and deflocculation, while US-induced cavitation facilitated microbial cell lysis, collectively enhancing hydrolysis. These findings demonstrate that ASA–US pretreatment is an effective and synergistic strategy to improve methane recovery from waste activated sludge.
厌氧消化被广泛用于稳定废活性污泥和回收甲烷,但其效率受到复杂有机物水解缓慢的限制。本研究研究了一种新的联合预处理方法,利用抗坏血酸(ASA)和超声波(US)来提高污泥的增溶性和甲烷产量。从溶解COD、生物聚合物释放、甲烷产量和微生物群落转移等方面评价了ASA、US及其整合(ASA - US)的有效性。最佳联合预处理(0.04 g/g TSS - ASA和1.625 W/mL US)的COD溶出率为21.7%,高于单独使用ASA(7.6%)和US(14.2%)。与对照组相比,甲烷产量增加了109.6%,ASA-US预处理污泥的甲烷产量达到285±4 mL/g VS。动力学模型(修正的Gompertz和Logistic模型)证实了预处理污泥的生物降解性增强。微生物分析显示,水解和产酸类群如厚壁菌门和梭状芽胞杆菌的显著富集,同时变形菌门的减少,表明群落转向有利于产甲烷。在机制上,ASA促进了EPS的破坏和反絮凝,而us诱导的空化促进了微生物细胞的裂解,共同促进了水解。上述结果表明,ASA-US预处理是提高废活性污泥甲烷回收率的有效协同策略。
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引用次数: 0
Corrigendum to “Separation and purification of N-acetylglucosamine from the fermentation broth of corynebacterium glutamicum by electrodeionisation method” [Biomass Bioenergy 202 (2025) 108165] “用电去离子法从谷氨酸棒状杆菌发酵液中分离和纯化n -乙酰氨基葡萄糖”的勘误表[生物质生物能源202 (2025)108165]
IF 6 2区 生物学 Q1 AGRICULTURAL ENGINEERING Pub Date : 2026-01-13 DOI: 10.1016/j.biombioe.2025.108924
Xiqin Zhou, Hao He, Minxuan Wang, Zemin Li, Jie Wei, Gaojie Pan, Hui Cao, Tianwei Tan
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引用次数: 0
Discovery of a lignocellulosic hydrolysate-tolerant Saccharomyces cerevisiae strain and elucidation of a novel stress resistance mechanism 一株木质纤维素水解耐受性酿酒酵母菌的发现及一种新的抗逆性机制的阐明
IF 6 2区 生物学 Q1 AGRICULTURAL ENGINEERING Pub Date : 2026-01-12 DOI: 10.1016/j.biombioe.2026.108941
Zhengyue Zhang, Linjia Jiang, Hanyu Wang, Qian Li, Sardar Ali, Yulei Chen, Jiaye Tang, Jiwei Shen, Wenli Xin, Lingling Feng, Menggen Ma
Lignocellulosic hydrolysates are rich in inhibitory compounds, which severely hinder the performance of Saccharomyces cerevisiae in bio-based production processes. In this study, we employed adaptive laboratory evolution (ALE) over 90 serial transfers under increasing inhibitor concentrations to generate strain 40B, exhibiting broad-spectrum tolerance. Phenotypic analysis revealed significantly improved growth and fermentation performance in high-inhibitor hydrolysate conditions. Integrated genomic and transcriptomic analyses identified key tolerance mechanisms, including enhanced antioxidant defense, energy metabolism, membrane integrity, and notably, the upregulation of amide-tRNA synthetases—a previously unreported adaptation in yeast. These changes supported elevated TCA cycle activity, reduced ROS levels, and improved organelle stability under inhibitor stress. Overexpression of YEF1, FDH1, and CRZ1 conferred increased inhibitor tolerance, while mutations in PTC4, ISC1, and GPA1 were found to be pivotal in modulating stress responses. This finding advances microbial stress response understanding and addresses gaps in designing robust microbial cell factories for sustainable biomass conversion.
木质纤维素水解物富含抑制化合物,严重阻碍了酿酒酵母在生物基生产过程中的性能。在本研究中,我们采用适应性实验室进化(ALE)方法,在增加抑制剂浓度的条件下,经过90次连续转移,产生了具有广谱耐受性的菌株40B。表型分析显示,在高抑制剂水解条件下,生长和发酵性能显著提高。整合基因组学和转录组学分析确定了关键的耐受性机制,包括增强抗氧化防御,能量代谢,膜完整性,特别是酰胺- trna合成酶的上调,这是酵母中以前未报道的适应性。这些变化支持在抑制剂胁迫下TCA循环活性升高、ROS水平降低和细胞器稳定性改善。YEF1、FDH1和CRZ1的过表达增加了抑制剂的耐受性,而PTC4、ISC1和GPA1的突变被发现在调节应激反应中起关键作用。这一发现促进了对微生物应激反应的理解,并解决了设计稳健的微生物细胞工厂以实现可持续生物质转化的空白。
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引用次数: 0
Green one-step valorization of biomass residue into tunable nitrogen/sulfur dual-doped hierarchical porous carbon for eco-friendly energy storage 生物质残渣一步绿色增值成可调氮/硫双掺杂分层多孔碳用于生态能源储存
IF 5.8 2区 生物学 Q1 AGRICULTURAL ENGINEERING Pub Date : 2026-01-12 DOI: 10.1016/j.biombioe.2026.108971
Zhong-Tao Xiong, Xiao-Fan Wu, Min Yang
Developing green pathways to convert biomass residues into advanced carbon materials plays a crucial role in promoting carbon neutrality and renewable energy utilization. In this study, bamboo-derived residues were adopted as the carbon feed material, L-methionine provided nitrogen and sulfur functionalities, and KMnO4 served as a co-activating agent. The proportional control strategy coupled with one-step carbonization enabled the formation of coral-like carbon architectures (BWC-Met) with N and S dual incorporation and cross-linked porosity. BWC-Met-2 outperformed its counterparts owing to its high accessible surface (1385.62 m2 g−1) and superior charge-storage capability (460.2 F g−1 at 1 A g−1). At a power density of 500 W kg−1, the symmetric supercapacitor assembled from BWC-Met-2 exhibited an energy density of 8.6 Wh·kg−1. Even after 10,000 charge-discharge cycles, the device preserved 94.2 % of its initial capacitance, indicating outstanding cycling durability. The proposed green and straightforward fabrication approach enables controllable generation of porous carbon frameworks from renewable biomass residues, while simultaneously enhancing the capacitive output of the devices. This strategy provides a promising avenue for advancing bio-derived carbon materials toward next-generation energy storage applications.
开发将生物质残渣转化为先进碳材料的绿色途径对促进碳中和和可再生能源利用具有重要作用。本研究以竹基残基为碳料,l -蛋氨酸提供氮和硫官能团,KMnO4作为共活化剂。比例控制策略与一步炭化相结合,形成了具有N和S双重结合和交联孔隙度的类珊瑚碳结构(BWC-Met)。BWC-Met-2由于其高可达表面(1385.62 m2 g−1)和优越的电荷存储能力(在1 A g−1时为460.2 F g−1)而优于同类材料。在功率密度为500 W kg−1时,BWC-Met-2组装的对称超级电容器的能量密度为8.6 Wh·kg−1。即使在10,000次充放电循环后,该设备仍保留了94.2%的初始电容,表明其出色的循环耐久性。提出的绿色和直接的制造方法能够从可再生生物质残留物中可控地产生多孔碳框架,同时提高设备的电容输出。这一策略为推进生物衍生碳材料向下一代储能应用提供了一条有前途的途径。
{"title":"Green one-step valorization of biomass residue into tunable nitrogen/sulfur dual-doped hierarchical porous carbon for eco-friendly energy storage","authors":"Zhong-Tao Xiong,&nbsp;Xiao-Fan Wu,&nbsp;Min Yang","doi":"10.1016/j.biombioe.2026.108971","DOIUrl":"10.1016/j.biombioe.2026.108971","url":null,"abstract":"<div><div>Developing green pathways to convert biomass residues into advanced carbon materials plays a crucial role in promoting carbon neutrality and renewable energy utilization. In this study, bamboo-derived residues were adopted as the carbon feed material, <em>L</em>-methionine provided nitrogen and sulfur functionalities, and KMnO<sub>4</sub> served as a co-activating agent. The proportional control strategy coupled with one-step carbonization enabled the formation of coral-like carbon architectures (BWC-Met) with N and S dual incorporation and cross-linked porosity. BWC-Met-2 outperformed its counterparts owing to its high accessible surface (1385.62 m<sup>2</sup> g<sup>−1</sup>) and superior charge-storage capability (460.2 F g<sup>−1</sup> at 1 A g<sup>−1</sup>). At a power density of 500 W kg<sup>−1</sup>, the symmetric supercapacitor assembled from BWC-Met-2 exhibited an energy density of 8.6 Wh·kg<sup>−1</sup>. Even after 10,000 charge-discharge cycles, the device preserved 94.2 % of its initial capacitance, indicating outstanding cycling durability. The proposed green and straightforward fabrication approach enables controllable generation of porous carbon frameworks from renewable biomass residues, while simultaneously enhancing the capacitive output of the devices. This strategy provides a promising avenue for advancing bio-derived carbon materials toward next-generation energy storage applications.</div></div>","PeriodicalId":253,"journal":{"name":"Biomass & Bioenergy","volume":"209 ","pages":"Article 108971"},"PeriodicalIF":5.8,"publicationDate":"2026-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145962540","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Introducing chloroform as cyclopropanation precursor for the efficient synthesis of high-energy fuels 介绍了氯仿作为高效合成高能燃料的环丙烷前体
IF 5.8 2区 生物学 Q1 AGRICULTURAL ENGINEERING Pub Date : 2026-01-12 DOI: 10.1016/j.biombioe.2026.108945
Wenbin Huang , Jiahao Wang , Liyu Chang , Hui Li , Long Liu , Yanqiang Zhang
Cyclopropanation of biomass alkenes is a novel method for the high-energy rocket fuel preparation. Here we report a pathway for the rapid access to cyclopropane-based fuels, i.e., dichlorocyclopropanation of alkenes and dechlorination. Specifically, CHCl3 is deprotonated and dechlorinated to generate highly active dichlorocarbene. The subsequent cyclopropanation of biomass monoalkene (β-pinene), dialkene (limonene) and trialkene (myrcene) with dichlorocarbene affords dichlorocyclopropane compounds, which are dechlorinated by the metal sodium to get three high energetic fuels (ρ: 0.84–0.89 g mL−1, 42.75–43.46 MJ kg−1). The resulted monocyclopropane and dicyclopropane-based fuels have the two-step yields as 84 % and 76 %, while lower yield for tricyclopropane as 42 %. Our research provides a straightforward method for the synthesis of high-energy rocket fuels, which exhibits the competitive potential for industrial applications.
生物质烯烃环丙烷化是制备高能火箭燃料的一种新方法。在这里,我们报告了快速获取环丙烷基燃料的途径,即烯烃的二氯环丙烷化和脱氯。具体来说,CHCl3被去质子化和去氯化,生成高活性的二氯苯。生物质单烯(β-蒎烯)、二烯(柠檬烯)和三烯(myrcene)与二氯甲烷进行环丙烷反应,得到二氯环丙烷化合物,金属钠对其进行脱氯,得到三种高能燃料(ρ: 0.84-0.89 g mL−1,42.75-43.46 MJ kg−1)。所得单环丙烷和双环丙烷基燃料的两步产率分别为84%和76%,而三环丙烷的两步产率较低,为42%。我们的研究为高能火箭燃料的合成提供了一种简单的方法,具有工业应用的竞争潜力。
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引用次数: 0
Enzyme-mimetic nanomaterials trigger transcriptional activation of xylose-utilizing genes in Saccharomyces cerevisiae for enhanced lignocellulosic bioethanol production 模拟酶纳米材料触发酿酒酵母中木糖利用基因的转录激活,以增强木质纤维素生物乙醇的生产
IF 5.8 2区 生物学 Q1 AGRICULTURAL ENGINEERING Pub Date : 2026-01-12 DOI: 10.1016/j.biombioe.2026.108944
Mamata S. Singhvi , Beom Soo Kim
In this study, the primary aim was to establish an environmentally sustainable method for converting corn cob biomass waste (CCBW) into simple sugars using CeFe3O4NPs through the simultaneous pretreatment and hydrolysis (SPH) process at the fermenter level. The hydrolysis reaction using NPs yielded a maximum of 5.95 ± 0.102 g/L glucose and 4.22 ± 0.143 g/L xylose within 24 h at 50 °C in a 7.0 L fermenter scale. The released sugars during the SPH process explain how CeFe3O4NPs work as oxidase and cellulase-hemicellulase enzymes. The study also investigates the auxiliary effect of enzymes in combination with CeFe3O4NPs on raw CCBW hydrolysis. The small amounts of in-house generated cellulase-hemicellulase enzymes were used, resulting in higher glucose levels (20.10 ± 1.12 g/L) and xylose (14.9 ± 0.88 g/L) sugars being liberated. Saccharomyces cerevisiae converted these sugars into 15.2 ± 0.87 g/L of bioethanol with a productivity rate of 1.27 g/L/h using a hydrolysate. The presence of CeFe3O4NPs has led to the use of xylose in S. cerevisiae, a new observation that highlights the potential role of CeFe3O4NPs in enhancing the expression of genes responsible for xylose utilization. Transcriptomic analysis of yeast cells treated with CeFe3O4NPs confirmed upregulation of key xylose-utilizing genes, XKS1 and YJR096W, which was validated by real-time PCR. This is the first report on such studies proving the role of CeFe3O4NPs in upregulating important xylose-utilizing genes, namely Xylulokinase (XKS1) and Xylose reductase (XR, YJR096W). This study could be a breakthrough in producing 2G biofuels by utilizing both C5 and C6 sugars at commercial scale.
在本研究中,主要目的是建立一种环境可持续的方法,通过发酵水平的预处理和水解(SPH)过程,利用CeFe3O4NPs将玉米芯生物质废物(CCBW)转化为单糖。在7.0 L发酵规模下,在50°C条件下,24 h内,NPs水解得到葡萄糖5.95±0.102 g/L,木糖4.22±0.143 g/L。SPH过程中释放的糖解释了CeFe3O4NPs如何作为氧化酶和纤维素酶-半纤维素酶起作用。本研究还考察了酶与CeFe3O4NPs结合对原CCBW水解的辅助作用。使用少量内部生成的纤维素酶-半纤维素酶,导致更高的葡萄糖水平(20.10±1.12 g/L)和木糖(14.9±0.88 g/L)被释放。酿酒酵母利用水解产物将这些糖转化为15.2±0.87 g/L的生物乙醇,产率为1.27 g/L/h。CeFe3O4NPs的存在导致酿酒酵母对木糖的利用,这一新的观察结果强调了CeFe3O4NPs在增强木糖利用基因表达方面的潜在作用。用CeFe3O4NPs处理酵母细胞的转录组学分析证实了关键木糖利用基因XKS1和YJR096W的上调,并通过实时PCR验证了这一点。这是同类研究中首次证实CeFe3O4NPs在木糖激酶(XKS1)和木糖还原酶(XR, YJR096W)等重要木糖利用基因上调中的作用。这项研究可能是利用C5和C6糖在商业规模上生产2G生物燃料的突破。
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引用次数: 0
Low intensity microwave assisted bacterial pretreatment of food waste for energy efficient biomethanation 低强度微波辅助细菌预处理食物垃圾的节能生物甲烷化
IF 5.8 2区 生物学 Q1 AGRICULTURAL ENGINEERING Pub Date : 2026-01-12 DOI: 10.1016/j.biombioe.2026.108947
S. Kavitha , S. Bharathi , Yukesh Kannah Ravi , V. Pugalenthi , J. Rajesh Banu
The complex nature and high organic content of Food Waste (FW) are responsible for mass transfer limitations during biological pretreatment (BP), which in turn reduces the biomethane yield during subsequent anaerobic digestion (AD). A novel energy-efficient microwave (MW) mediated BP was developed as an alternative to conventional energy-intensive pretreatments to enhance the FW hydrolysis and to overcome the limitation of BP to achieve greater biomethane yield. Microwave treatment (MWT) was used as a first-stage treatment, and the lowest specific energy (SE) of 14.4, 36.0, 76.8, and 86.4 MJ/kg TS were required to achieve 5, 10, 15, and 20 % Chemical Oxygen Demand (COD) solubilization respectively. Upon subjecting this condition to BP, MWT+BP with 5 % COD solubilization significantly improved FW hydrolysis from 5.0 to 16.5 % with relatively low pretreatment energy (14.8 MJ/kg TS). However, other conditions (MWT+BP with 10, 15, and 20 %) resulted in a marginal increase in COD solubilization (17.0, 19.0, and 22.1 %, respectively), yet they required higher input energy (36.4, 77.2, and 86.8 MJ/kg TS, respectively). This indicates that the differences in biogas yield among MWT+BP samples (5, 10, 15, and 20 %) were insignificant (368, 383, 409, and 439 mL/gVS) and did not compensate for the additional energy they warranted during MWT. The net energy to valorize FW was calculated to be only positive for MWT+BP with 5 % solubilization (+298 MJ). It achieves an energy ratio greater than 1, making the process scalable and efficient.
食物垃圾(FW)的复杂性质和高有机含量是生物预处理(BP)过程中传质受限的原因,这反过来又降低了随后厌氧消化(AD)过程中的生物甲烷产量。研究了一种新型的微波介导BP技术,以提高FW的水解效率,克服BP技术的局限性,从而提高生物甲烷的产量。第一阶段采用微波处理(MWT),最低比能(SE)分别为14.4、36.0、76.8和86.4 MJ/kg TS,可实现5%、10%、15%和20%的化学需氧量(COD)增溶。在此条件下,添加5% COD的MWT+BP在相对较低的预处理能量(14.8 MJ/kg TS)下显著提高了FW的水解率,从5.0提高到16.5%。然而,其他条件(MWT+BP分别为10%、15%和20%)对COD的增溶作用略有增加(分别为17.0%、19.0%和22.1%),但它们需要更高的输入能量(分别为36.4、77.2和86.8 MJ/kg TS)。这表明MWT+BP样品(5%、10%、15%和20%)的沼气产量差异不显著(368,383,409和439 mL/gVS),并且不能补偿它们在MWT期间所需的额外能量。经计算,当增溶量为5% (+298 MJ)时,MWT+BP的净能仅为正。它实现了大于1的能量比,使该过程可扩展且高效。
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Biomass & Bioenergy
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