Pub Date : 2026-02-02DOI: 10.1016/j.biombioe.2026.109043
Fazal Um Min Allah, Rodrigo Nogueira De Sousa, Elena Trim, Adriano da Silva Marques, Monica Carvalho
{"title":"Techno-economic assessment of bioenergy with carbon capture and storage for Brazilian thermoelectric power plants","authors":"Fazal Um Min Allah, Rodrigo Nogueira De Sousa, Elena Trim, Adriano da Silva Marques, Monica Carvalho","doi":"10.1016/j.biombioe.2026.109043","DOIUrl":"https://doi.org/10.1016/j.biombioe.2026.109043","url":null,"abstract":"","PeriodicalId":253,"journal":{"name":"Biomass & Bioenergy","volume":"18 1","pages":""},"PeriodicalIF":6.0,"publicationDate":"2026-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146110593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Comprehensive experimental analysis of pyrolytic gas composition from various lignocellulosic biomass","authors":"Silvia Pena Meneses, Jean-François Largeau, Khaled Loubar, François Ricoul, Jérôme Bellettre","doi":"10.1016/j.biombioe.2026.109038","DOIUrl":"https://doi.org/10.1016/j.biombioe.2026.109038","url":null,"abstract":"","PeriodicalId":253,"journal":{"name":"Biomass & Bioenergy","volume":"176 1","pages":""},"PeriodicalIF":6.0,"publicationDate":"2026-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146110830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-02-02DOI: 10.1016/j.biombioe.2026.109039
Elianny Da Silva, Juan A. Cecilia, Benjamin Torres-Olea, Mahesh Eledath-Changarath, Marie Krečmarová, Juan F. Sánchez-Royo, Rita Sánchez-Tovar, Adrian García, Benjamin Solsona
{"title":"Valorization of biomass-derived compounds using residual sludge from water treatment as a catalytic support","authors":"Elianny Da Silva, Juan A. Cecilia, Benjamin Torres-Olea, Mahesh Eledath-Changarath, Marie Krečmarová, Juan F. Sánchez-Royo, Rita Sánchez-Tovar, Adrian García, Benjamin Solsona","doi":"10.1016/j.biombioe.2026.109039","DOIUrl":"https://doi.org/10.1016/j.biombioe.2026.109039","url":null,"abstract":"","PeriodicalId":253,"journal":{"name":"Biomass & Bioenergy","volume":"89 1","pages":""},"PeriodicalIF":6.0,"publicationDate":"2026-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146110594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-02-02DOI: 10.1016/j.biombioe.2026.109025
Bisheswar Karmakar, Rishya Prava Chatterjee, Rhithuparna D., Sankha Chakrabortty, Rashid Imran Ahmad Khan, Gopinath Halder
Catalysed biodiesel synthesis requires an extended duration and produces large amounts of wastewater while incurring significant catalyst costs. In contrast, the uncatalysed conversion of suitable feedstock using superheated alcohols can provide sufficient energy, which means activation energy no longer affects reactivity. This eliminates catalyst requirement in addition to drastically reducing reaction time, as well as simplifying product separation and recovery of alcohols or by-products. The current study reports on uncatalysed alcoholysis of a binary blend made from mahua oil (MI) and waste cooking oil (WCO) to synthesize biodiesel using superheated blends of methanol and 2-propanol. Batch experiments identified ranges for six parameters suitable for soft-computational optimization and these parameters were then optimized via central composite design (CCD). A biodiesel yield of 98.9 % (predicted yield of 99.03 %) could be attained by reacting 600 g of WCO-MI oil blend in a 3:1 ratio using methanol and 2-propanol in a 6:5 ratio after preheating the alcohol mixture to 135 °C. The reaction proceeded optimally at 250 °C, taking 8 min retention time to be completed. Experimental results were analysed for reliability using ANOVA study, and data validation was carried out using artificial neural network (ANN). From ANOVA studies, it is seen that retention time has the most prominent impact on the process with a contribution factor of 48.82 %, while methanol to 2-propanol ratio has the lowest contribution factor of 0.25 %. The ANN model established adopting a 6-10-1 algorithm showed an R2 value of 0.987, thereby reaching great accuracy which confirms its competence for reliable predictions.
催化合成生物柴油需要较长的时间，产生大量的废水，同时产生大量的催化剂成本。相反，使用过热的醇对合适的原料进行无催化转化可以提供足够的能量，这意味着活化能不再影响反应性。这消除了催化剂的需求，除了大大减少反应时间，以及简化产品分离和回收的醇或副产物。本研究报道了用甲醇和2-丙醇的过热混合物对麻花油（MI）和废食用油（WCO）二元混合物进行无催化醇解合成生物柴油。批量实验确定了适合软计算优化的6个参数范围，并通过中心复合设计（CCD）对这些参数进行优化。将600 g WCO-MI油以3:1的比例与甲醇和2-丙醇以6:5的比例反应，将酒精混合物预热至135℃，可获得98.9%的生物柴油产率（预测产率为99.03%）。反应在250°C下进行最佳，保留时间为8 min。采用方差分析对实验结果进行信度分析，并采用人工神经网络（ANN）对数据进行验证。由方差分析可知，保留时间对工艺的影响最为突出，贡献因子为48.82%，而甲醇与2-丙醇比的贡献因子最低，为0.25%。采用6-10-1算法建立的人工神经网络模型的R2值为0.987，具有较高的准确率，证实了其可靠预测的能力。
{"title":"Superheated non-catalytic transesterification of binary mixture of Madhuca indica and waste cooking oil towards biodiesel synthesis","authors":"Bisheswar Karmakar, Rishya Prava Chatterjee, Rhithuparna D., Sankha Chakrabortty, Rashid Imran Ahmad Khan, Gopinath Halder","doi":"10.1016/j.biombioe.2026.109025","DOIUrl":"https://doi.org/10.1016/j.biombioe.2026.109025","url":null,"abstract":"Catalysed biodiesel synthesis requires an extended duration and produces large amounts of wastewater while incurring significant catalyst costs. In contrast, the uncatalysed conversion of suitable feedstock using superheated alcohols can provide sufficient energy, which means activation energy no longer affects reactivity. This eliminates catalyst requirement in addition to drastically reducing reaction time, as well as simplifying product separation and recovery of alcohols or by-products. The current study reports on uncatalysed alcoholysis of a binary blend made from mahua oil (MI) and waste cooking oil (WCO) to synthesize biodiesel using superheated blends of methanol and 2-propanol. Batch experiments identified ranges for six parameters suitable for soft-computational optimization and these parameters were then optimized via central composite design (CCD). A biodiesel yield of 98.9 % (predicted yield of 99.03 %) could be attained by reacting 600 g of WCO-MI oil blend in a 3:1 ratio using methanol and 2-propanol in a 6:5 ratio after preheating the alcohol mixture to 135 °C. The reaction proceeded optimally at 250 °C, taking 8 min retention time to be completed. Experimental results were analysed for reliability using ANOVA study, and data validation was carried out using artificial neural network (ANN). From ANOVA studies, it is seen that retention time has the most prominent impact on the process with a contribution factor of 48.82 %, while methanol to 2-propanol ratio has the lowest contribution factor of 0.25 %. The ANN model established adopting a 6-10-1 algorithm showed an R<ce:sup loc=\"post\">2</ce:sup> value of 0.987, thereby reaching great accuracy which confirms its competence for reliable predictions.","PeriodicalId":253,"journal":{"name":"Biomass & Bioenergy","volume":"74 1","pages":""},"PeriodicalIF":6.0,"publicationDate":"2026-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146098297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-02-01DOI: 10.1016/j.biombioe.2026.109040
Roberto Carlos Campos-Flores, Santiago Iván Suárez-Vázquez, Eric Trably, Nicolas Bernet, Arquímedes Cruz-López
The production of biofuels from lignocellulosic biomass is one of the most promising alternatives for generating clean energy. However, various challenges arise when using this recalcitrant biomass in biotechnological processes for the production of biofuels (ethanol, biogas, biohydrogen, and methane), such as low yields due to the structural complexity of lignocellulose. In this sense, this study evaluated a biological pretreatment of a lignocellulosic waste mixture consisting of fruit peels (FRP) and brewer's spent grain (BSG), through solid-state fermentation (SSF) using the white-rot fungi Trametes versicolor and Pleurotus ostreatus. Different pretreatment conditions were assessed, including duration (15, 25, and 35 days), temperature (28 and 32 °C), and fungal strain (T. versicolor and P. ostreatus), to determine their impact on reducing sugars concentration and delignification and improving the bioaccesibility of the biomass in further bioenergy obtention process.
{"title":"White-rot fungi as a sustainable pretreatment approach to improve lignocellulosic biomass conversion for biohydrogen production","authors":"Roberto Carlos Campos-Flores, Santiago Iván Suárez-Vázquez, Eric Trably, Nicolas Bernet, Arquímedes Cruz-López","doi":"10.1016/j.biombioe.2026.109040","DOIUrl":"https://doi.org/10.1016/j.biombioe.2026.109040","url":null,"abstract":"The production of biofuels from lignocellulosic biomass is one of the most promising alternatives for generating clean energy. However, various challenges arise when using this recalcitrant biomass in biotechnological processes for the production of biofuels (ethanol, biogas, biohydrogen, and methane), such as low yields due to the structural complexity of lignocellulose. In this sense, this study evaluated a biological pretreatment of a lignocellulosic waste mixture consisting of fruit peels (FRP) and brewer's spent grain (BSG), through solid-state fermentation (SSF) using the white-rot fungi <ce:italic>Trametes versicolor</ce:italic> and <ce:italic>Pleurotus ostreatus</ce:italic>. Different pretreatment conditions were assessed, including duration (15, 25, and 35 days), temperature (28 and 32 °C), and fungal strain (<ce:italic>T. versicolor</ce:italic> and <ce:italic>P. ostreatus</ce:italic>), to determine their impact on reducing sugars concentration and delignification and improving the bioaccesibility of the biomass in further bioenergy obtention process.","PeriodicalId":253,"journal":{"name":"Biomass & Bioenergy","volume":"275 1","pages":""},"PeriodicalIF":6.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146098298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-01-31DOI: 10.1016/j.biombioe.2026.109012
Ana Luiza Slama de Freitas , Tim G.W. Engels , Salvador Bertran Llorens , Alessia Lasorsa , Patrick C.A. van der Wel , Hero J. Heeres , Peter J. Deuss
Specific wastewater streams from wastewater treatment facilities contain components with significant valorization potential, including cellulosic material derived from waste toilet paper. Quantifying cellulose and other carbohydrate content and identifying impurities is essential for developing efficient valorization strategies. In this study, tertiary cellulose (Recell®) obtained from a wastewater treatment facility was characterized to determine its overall chemical composition. Extraction with hexane, ethanol, and water gave a extractives removal of 9.3, 2.8, and 2.9 wt%, respectively mostly identified as fatty acids and esters (GC-MS). Detailed analyses of the extractive-free samples revealed the presence of cellulose (60 wt%), xylans (12 wt%), ash (8 wt%), proteins (5 wt%), and 2.5 wt% of residue after acid hydrolysis, which is normally associated with lignin. However, it was unequivocally proven (NMR, py-GC-MS) that lignin is not present and that this residue can be in part associated with plastic particles and proteins. After enzymatic digestion (48 h batch time), the glucose yield was 45 wt% for crude Recell® and 54 wt% for the extractive-free samples of the feedstock, and 81 and 90 wt% based on cellulose content, respectively. This shows that Recell® material is particularly cellulose-rich and that it can be readily converted into glucose. Removal of extractives can further enhance the enzymatic hydrolysis to facilitate valorization of this abundant residue stream.
{"title":"In-depth characterization of tertiary cellulose from wastewater treatment facilities","authors":"Ana Luiza Slama de Freitas , Tim G.W. Engels , Salvador Bertran Llorens , Alessia Lasorsa , Patrick C.A. van der Wel , Hero J. Heeres , Peter J. Deuss","doi":"10.1016/j.biombioe.2026.109012","DOIUrl":"10.1016/j.biombioe.2026.109012","url":null,"abstract":"<div><div>Specific wastewater streams from wastewater treatment facilities contain components with significant valorization potential, including cellulosic material derived from waste toilet paper. Quantifying cellulose and other carbohydrate content and identifying impurities is essential for developing efficient valorization strategies. In this study, tertiary cellulose (Recell®) obtained from a wastewater treatment facility was characterized to determine its overall chemical composition. Extraction with hexane, ethanol, and water gave a extractives removal of 9.3, 2.8, and 2.9 wt%, respectively mostly identified as fatty acids and esters (GC-MS). Detailed analyses of the extractive-free samples revealed the presence of cellulose (60 wt%), xylans (12 wt%), ash (8 wt%), proteins (5 wt%), and 2.5 wt% of residue after acid hydrolysis, which is normally associated with lignin. However, it was unequivocally proven (NMR, py-GC-MS) that lignin is not present and that this residue can be in part associated with plastic particles and proteins. After enzymatic digestion (48 h batch time), the glucose yield was 45 wt% for crude Recell® and 54 wt% for the extractive-free samples of the feedstock, and 81 and 90 wt% based on cellulose content, respectively. This shows that Recell® material is particularly cellulose-rich and that it can be readily converted into glucose. Removal of extractives can further enhance the enzymatic hydrolysis to facilitate valorization of this abundant residue stream.</div></div>","PeriodicalId":253,"journal":{"name":"Biomass & Bioenergy","volume":"210 ","pages":"Article 109012"},"PeriodicalIF":5.8,"publicationDate":"2026-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146089480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-01-31DOI: 10.1016/j.biombioe.2026.109029
Xiaodong Chu, Jianwu Zhang, Yongdong Chen, Zhenyan Liu, Zhi Wang
{"title":"Enhancement techniques for anaerobic digestion of lignocellulosic biomass: a review on substrate pre-regulation and process enhancement","authors":"Xiaodong Chu, Jianwu Zhang, Yongdong Chen, Zhenyan Liu, Zhi Wang","doi":"10.1016/j.biombioe.2026.109029","DOIUrl":"https://doi.org/10.1016/j.biombioe.2026.109029","url":null,"abstract":"","PeriodicalId":253,"journal":{"name":"Biomass & Bioenergy","volume":"91 1","pages":""},"PeriodicalIF":6.0,"publicationDate":"2026-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146095823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study investigated the efficacy of bioaugmentation in enhancing biomethane yield from the anaerobic co-digestion of carbohydrate- and lipid-protein-rich wastes with sewage sludge. Four microbial treatments were evaluated: two novel strains isolated from anaerobic sludge (Bacillus sp. MH1 and Serratia sp. MH2) and two reference strains (Lactobacillus casei and Saccharomyces cerevisiae). These were tested individually and as two defined consortia (L. casei-MH1 and S. cerevisiae-MH2) in batch reactors under mesophilic conditions (35 °C) over 150 days. The MH1 treatment, both as a pure culture and in a consortium, demonstrated superior performance. Its success is mechanistically linked to proficient enzymatic hydrolysis of proteins via serine proteases, leading to a diverse profile of fermentation intermediates and the establishment of lactate-driven syntrophic partnerships. Conversely, the MH2 treatment significantly enhanced methane yields by facilitating the breakdown of recalcitrant lignocellulosic compounds, generating key precursors like acetate and propionate, and promoting alcohol-based syntrophy. The inclusion of reference strains, however, was correlated with a reduced hydrolysis rate. Kinetic analyses revealed that bioaugmentation with the isolated strains altered the fermentation dynamics, shifting the cumulative biogas production from a sigmoidal to a stepped profile. This was characterized by two distinct lag and logarithmic phases, indicating a more complex microbial succession that challenges conventional kinetic modeling. The MH1 treatment achieved the highest cumulative biomethane volume (12,244 mL), specific yield (0.608 m3/kg-VSdegraded), and volatile solids reduction (68 %). Kinetic parameters confirmed its superiority, exhibiting the maximum hydrolysis rate constant (k = 0.022 d−1) and daily biogas production rate (Rm = 6.6 m3/kg-VSdegraded.d).
{"title":"Bioaugmentation with novel Bacillus and Seramator strains enhances biomethane yield in anaerobic co-digestion of carbohydrate/lipid-protein wastes","authors":"Milad Hasani-Andevari , Bahar Shahnavaz , Mohsen Karrabi , Raf Dewil","doi":"10.1016/j.biombioe.2025.108906","DOIUrl":"10.1016/j.biombioe.2025.108906","url":null,"abstract":"<div><div>This study investigated the efficacy of bioaugmentation in enhancing biomethane yield from the anaerobic co-digestion of carbohydrate- and lipid-protein-rich wastes with sewage sludge. Four microbial treatments were evaluated: two novel strains isolated from anaerobic sludge (<em>Bacillus</em> sp. MH1 and <em>Serratia</em> sp. MH2) and two reference strains (<em>Lactobacillus casei</em> and <em>Saccharomyces cerevisiae</em>). These were tested individually and as two defined consortia (<em>L. casei</em>-MH1 and <em>S. cerevisiae</em>-MH2) in batch reactors under mesophilic conditions (35 °C) over 150 days. The MH1 treatment, both as a pure culture and in a consortium, demonstrated superior performance. Its success is mechanistically linked to proficient enzymatic hydrolysis of proteins via serine proteases, leading to a diverse profile of fermentation intermediates and the establishment of lactate-driven syntrophic partnerships. Conversely, the MH2 treatment significantly enhanced methane yields by facilitating the breakdown of recalcitrant lignocellulosic compounds, generating key precursors like acetate and propionate, and promoting alcohol-based syntrophy. The inclusion of reference strains, however, was correlated with a reduced hydrolysis rate. Kinetic analyses revealed that bioaugmentation with the isolated strains altered the fermentation dynamics, shifting the cumulative biogas production from a sigmoidal to a stepped profile. This was characterized by two distinct lag and logarithmic phases, indicating a more complex microbial succession that challenges conventional kinetic modeling. The MH1 treatment achieved the highest cumulative biomethane volume (12,244 mL), specific yield (0.608 m<sup>3</sup>/kg-VS<sub>degraded</sub>), and volatile solids reduction (68 %). Kinetic parameters confirmed its superiority, exhibiting the maximum hydrolysis rate constant (k = 0.022 d<sup>−1</sup>) and daily biogas production rate (R<sub>m</sub> = 6.6 m<sup>3</sup>/kg-VS<sub>degraded</sub>.d).</div></div>","PeriodicalId":253,"journal":{"name":"Biomass & Bioenergy","volume":"210 ","pages":"Article 108906"},"PeriodicalIF":5.8,"publicationDate":"2026-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146089484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-01-30DOI: 10.1016/j.biombioe.2026.108942
Evelin D.S.S. Franco , Danusia F. Lima , Camila P. Dantas , Ricardo de Souza Menezes , Olivia M.C. de Oliveira , Antônio FS. Queiroz , Ícaro T.A. Moreira
The enzyme alkane hydroxylase is useful in the recycling of petroleum effluents or compounds from the petroleum industry, such as wastewater, waste petroleum, and low molecular weight polyethylene. However, to minimize the generation of unwanted waste and promote a safe and sustainable return to the industry, optimizing the production and purification of the enzyme is essential. The main objective of this article was to present the results related to the optimization of enzyme production and purification. Optimization was performed using response surface methodology in the Origin 2026 program. The MINITAB® Release 22 software was used to generate three factorial designs and perform the Response Surface Methodology analysis. To purify this enzyme, three filtration steps were applied. The optimal activities for alkane hydroxylase in petroleum and glucose substrates were 94.3 U/L and 5.64 U/L, respectively, at temperatures of 35 °C and 30 °C, pH values of 7 and 7.5, with a maximum estimated at 7.33 and optimal oxygenation of 80 %. To avoid generating unnecessary waste for the same response, it was decided to work only with PD7 in the second experimental design. Enzymatic activity varied significantly with temperature for the enzyme produced in glucose by the RA2 strain, mainly at levels of 30–37 °C. For PD7, this variation occurred in relation to the pre-inoculum factor. Although the optimization procedure was effective in obtaining the purified enzyme, the specific activity was low. It is believed that adding another purification step could resolve this problem.
{"title":"Optimization of operational conditions for the production and purification of the alkane hydroxylase enzyme for Bioremediation of Petroleum Waste","authors":"Evelin D.S.S. Franco , Danusia F. Lima , Camila P. Dantas , Ricardo de Souza Menezes , Olivia M.C. de Oliveira , Antônio FS. Queiroz , Ícaro T.A. Moreira","doi":"10.1016/j.biombioe.2026.108942","DOIUrl":"10.1016/j.biombioe.2026.108942","url":null,"abstract":"<div><div>The enzyme alkane hydroxylase is useful in the recycling of petroleum effluents or compounds from the petroleum industry, such as wastewater, waste petroleum, and low molecular weight polyethylene. However, to minimize the generation of unwanted waste and promote a safe and sustainable return to the industry, optimizing the production and purification of the enzyme is essential. The main objective of this article was to present the results related to the optimization of enzyme production and purification. Optimization was performed using response surface methodology in the Origin 2026 program. The MINITAB® Release 22 software was used to generate three factorial designs and perform the Response Surface Methodology analysis. To purify this enzyme, three filtration steps were applied. The optimal activities for alkane hydroxylase in petroleum and glucose substrates were 94.3 U/L and 5.64 U/L, respectively, at temperatures of 35 °C and 30 °C, pH values of 7 and 7.5, with a maximum estimated at 7.33 and optimal oxygenation of 80 %. To avoid generating unnecessary waste for the same response, it was decided to work only with PD7 in the second experimental design. Enzymatic activity varied significantly with temperature for the enzyme produced in glucose by the RA2 strain, mainly at levels of 30–37 °C. For PD7, this variation occurred in relation to the pre-inoculum factor. Although the optimization procedure was effective in obtaining the purified enzyme, the specific activity was low. It is believed that adding another purification step could resolve this problem.</div></div>","PeriodicalId":253,"journal":{"name":"Biomass & Bioenergy","volume":"210 ","pages":"Article 108942"},"PeriodicalIF":5.8,"publicationDate":"2026-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146089482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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