Pub Date : 2025-03-18DOI: 10.1016/j.chemosphere.2025.144326
Dominika Sikora , Barbara Poniedziałek , Piotr Rzymski
Exposure to per- and polyfluoroalkyl substances (PFAS) through drinking water has emerged as a significant public health concern due to their persistent, bioaccumulative nature and adverse health effects. Drinking water is the primary non-occupational source of PFAS exposure. Recently, investigative journalism has alerted about the presumptive contamination of drinking water at various European sites, including Poland. However, direct data on PFAS concentrations in raw and treated drinking water in Poland remain scarce. Therefore, this study analyzed the concentrations of 20 PFAS in 18 drinking water samples from Poznań County in Greater Poland, where the contamination of drinking water with PFAS was also presumed. In most samples (14/18; 77.7 %), PFAS concentrations were below the method detection limit (<0.001 μg/L). Positive samples revealed total PFAS levels in the 0.0014–0.0041 μg/L range, decisively below the safety threshold established by the European Drinking Water Directive (<0.1 μg/L). Daily consumption of 1.5 or 2.0 L of water containing PFAS by a 70-kg adult would constitute 4.8–9.3 % or 6.4–18.6 % of tolerable intake set by the European Food Safety Authority. These findings suggest that drinking water in the studied area is not a significant source of PFAS exposure. Further research is recommended to assess PFAS contamination in drinking water across a broader geographic scope in Poland to better understand the national contamination status.
{"title":"Assessment of PFAS levels in drinking water: A case study from Poznań County (Poland)","authors":"Dominika Sikora , Barbara Poniedziałek , Piotr Rzymski","doi":"10.1016/j.chemosphere.2025.144326","DOIUrl":"10.1016/j.chemosphere.2025.144326","url":null,"abstract":"<div><div>Exposure to per- and polyfluoroalkyl substances (PFAS) through drinking water has emerged as a significant public health concern due to their persistent, bioaccumulative nature and adverse health effects. Drinking water is the primary non-occupational source of PFAS exposure. Recently, investigative journalism has alerted about the presumptive contamination of drinking water at various European sites, including Poland. However, direct data on PFAS concentrations in raw and treated drinking water in Poland remain scarce. Therefore, this study analyzed the concentrations of 20 PFAS in 18 drinking water samples from Poznań County in Greater Poland, where the contamination of drinking water with PFAS was also presumed. In most samples (14/18; 77.7 %), PFAS concentrations were below the method detection limit (<0.001 μg/L). Positive samples revealed total PFAS levels in the 0.0014–0.0041 μg/L range, decisively below the safety threshold established by the European Drinking Water Directive (<0.1 μg/L). Daily consumption of 1.5 or 2.0 L of water containing PFAS by a 70-kg adult would constitute 4.8–9.3 % or 6.4–18.6 % of tolerable intake set by the European Food Safety Authority. These findings suggest that drinking water in the studied area is not a significant source of PFAS exposure. Further research is recommended to assess PFAS contamination in drinking water across a broader geographic scope in Poland to better understand the national contamination status.</div></div>","PeriodicalId":276,"journal":{"name":"Chemosphere","volume":"377 ","pages":"Article 144326"},"PeriodicalIF":8.1,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143654527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-03-18DOI: 10.1016/j.chemosphere.2025.144318
Luong Van Duc, Daisuke Inoue, Michihiko Ike
This study investigated the combined and interactive effects of sulfate, salinity (NaCl), and ammonium on mesophilic anaerobic digestion using synthetic wastewater simulating concentrated municipal wastewater from the forward osmosis (FO) process. Batch anaerobic digestion experiments were conducted with varying concentrations of sulfate, NaCl, and ammonium. Complete sulfate reduction was observed in all test systems, regardless of the NaCl and ammonium concentration, indicating no significant inhibitory effect on sulfate-reducing bacteria (SRB). However, the increased toxicity of hydrogen sulfide produced by SRB under high concentrations of sulfate, NaCl, and ammonium inhibited methanogenic activity, resulting in reduced methane production. Despite this, methanogens, primarily Methanosarcina, tolerated low and moderate levels of sulfate, NaCl, and ammonium; thus, their coexistence with SRB (Desulfotomaculales) enabled efficient acetate utilization and methane production. The enhanced Methanosarcina activity was further confirmed through the antagonistic effects between NaCl and ammonium. No significant decrease in methane production was observed in the co-presence of 0.5 g/L sulfate, 10 g/L NaCl, and 1 g/L ammonium-nitrogen compared to the reference condition without the addition of these components. This study identified the inhibitory mechanisms resulting from sulfate, NaCl, and ammonium interactions, which may occur in FO-concentrated municipal wastewater. These findings offer insights for optimizing the FO process to maintain sulfate, NaCl, and ammonium concentrations below inhibitory levels, thereby ensuring efficient methane production.
{"title":"Combined inhibition of anaerobic digestion by sulfate, salinity, and ammonium: potential inhibitory factors in forward osmosis-concentrated municipal wastewater","authors":"Luong Van Duc, Daisuke Inoue, Michihiko Ike","doi":"10.1016/j.chemosphere.2025.144318","DOIUrl":"10.1016/j.chemosphere.2025.144318","url":null,"abstract":"<div><div>This study investigated the combined and interactive effects of sulfate, salinity (NaCl), and ammonium on mesophilic anaerobic digestion using synthetic wastewater simulating concentrated municipal wastewater from the forward osmosis (FO) process. Batch anaerobic digestion experiments were conducted with varying concentrations of sulfate, NaCl, and ammonium. Complete sulfate reduction was observed in all test systems, regardless of the NaCl and ammonium concentration, indicating no significant inhibitory effect on sulfate-reducing bacteria (SRB). However, the increased toxicity of hydrogen sulfide produced by SRB under high concentrations of sulfate, NaCl, and ammonium inhibited methanogenic activity, resulting in reduced methane production. Despite this, methanogens, primarily <em>Methanosarcina</em>, tolerated low and moderate levels of sulfate, NaCl, and ammonium; thus, their coexistence with SRB (Desulfotomaculales) enabled efficient acetate utilization and methane production. The enhanced <em>Methanosarcina</em> activity was further confirmed through the antagonistic effects between NaCl and ammonium. No significant decrease in methane production was observed in the co-presence of 0.5 g/L sulfate, 10 g/L NaCl, and 1 g/L ammonium-nitrogen compared to the reference condition without the addition of these components. This study identified the inhibitory mechanisms resulting from sulfate, NaCl, and ammonium interactions, which may occur in FO-concentrated municipal wastewater. These findings offer insights for optimizing the FO process to maintain sulfate, NaCl, and ammonium concentrations below inhibitory levels, thereby ensuring efficient methane production.</div></div>","PeriodicalId":276,"journal":{"name":"Chemosphere","volume":"377 ","pages":"Article 144318"},"PeriodicalIF":8.1,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143642066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Integrating carbon capture and utilization (CCU) technologies into wastewater treatment plants (WWTPs) is essential for mitigating greenhouse gas (GHG) emissions and enhancing environmental sustainability, but further advancements in process monitoring and control are critical to optimizing treatment performance. This study investigates the application of artificial intelligence (AI) modeling to enhance process monitoring and control in a novel integrated CCU biotechnology with a moving bed biofilm reactor (MBBR) sequenced with an algal photobioreactor (aPBR). This system reduces GHG and odour emissions simultaneously. Several machine learning (ML) models, including artificial neural networks (ANNs), support vector machines (SVM), random forest (RF), and least-squares boosting (LSBoost), were tested. The LSBoost was the most suitable for modeling the MBBR + aPBR system, exhibiting the highest accuracy in predicting CO2 (R2 = 0.97) and H2S (R2 = 0.95) emissions from the MBBR. LSBoost also achieved the highest accuracy for predicting CO2 (R2 = 0.85) and H2S (R2 = 0.97) outlet concentrations from the aPBR. These findings underscore the importance of aligning AI algorithms to the characteristics of the treatment technology. The proposed AI models outperformed conventional statistical methods, demonstrating their ability to capture the complex, nonlinear dynamics typical of processes in environmental technologies. This study highlights the potential of AI-driven monitoring and control systems to significantly improve the efficiency of CCU biotechnologies in WWTPs for climate change mitigation and sustainable wastewater management.
{"title":"Enhancing process monitoring and control in novel carbon capture and utilization biotechnology through artificial intelligence modeling: An advanced approach toward sustainable and carbon-neutral wastewater treatment","authors":"Stefano Cairone , Giuseppina Oliva , Fabiana Romano , Federica Pasquarelli , Aniello Mariniello , Antonis A. Zorpas , Simon J.T. Pollard , Kwang-Ho Choo , Vincenzo Belgiorno , Tiziano Zarra , Vincenzo Naddeo","doi":"10.1016/j.chemosphere.2025.144299","DOIUrl":"10.1016/j.chemosphere.2025.144299","url":null,"abstract":"<div><div>Integrating carbon capture and utilization (CCU) technologies into wastewater treatment plants (WWTPs) is essential for mitigating greenhouse gas (GHG) emissions and enhancing environmental sustainability, but further advancements in process monitoring and control are critical to optimizing treatment performance. This study investigates the application of artificial intelligence (AI) modeling to enhance process monitoring and control in a novel integrated CCU biotechnology with a moving bed biofilm reactor (MBBR) sequenced with an algal photobioreactor (aPBR). This system reduces GHG and odour emissions simultaneously. Several machine learning (ML) models, including artificial neural networks (ANNs), support vector machines (SVM), random forest (RF), and least-squares boosting (LSBoost), were tested. The LSBoost was the most suitable for modeling the MBBR + aPBR system, exhibiting the highest accuracy in predicting CO<sub>2</sub> (R<sup>2</sup> = 0.97) and H<sub>2</sub>S (R<sup>2</sup> = 0.95) emissions from the MBBR. LSBoost also achieved the highest accuracy for predicting CO<sub>2</sub> (R<sup>2</sup> = 0.85) and H<sub>2</sub>S (R<sup>2</sup> = 0.97) outlet concentrations from the aPBR. These findings underscore the importance of aligning AI algorithms to the characteristics of the treatment technology. The proposed AI models outperformed conventional statistical methods, demonstrating their ability to capture the complex, nonlinear dynamics typical of processes in environmental technologies. This study highlights the potential of AI-driven monitoring and control systems to significantly improve the efficiency of CCU biotechnologies in WWTPs for climate change mitigation and sustainable wastewater management.</div></div>","PeriodicalId":276,"journal":{"name":"Chemosphere","volume":"376 ","pages":"Article 144299"},"PeriodicalIF":8.1,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143637423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-03-17DOI: 10.1016/j.chemosphere.2025.144320
Isabella T. Tomasi , Inês Santos , Eren Gozubuyuk , Ounísia Santos , Rui A.R. Boaventura , Cidália M.S. Botelho
The global population faces increasing challenges in securing fresh water and food production. Aquaculture, a key source for obtaining protein, contributes significantly to environmental pollution, releasing suspended solids, organic matter, nitrogen, and phosphorus into water bodies. Some wastewater treatment methods can be expensive due to high equipment, energy, reagents, and maintenance costs, prompting the search for more sustainable alternatives. This study evaluates the performance of tannin-based coagulants from Castanea sativa shells (CS) for treating the recirculating water in an aquaculture plant. Two tannin-based coagulants, C1 and C2, were produced using ethanolamine (ETA) and NH4Cl, respectively. The efficiency in removing color, turbidity, organic matter, nitrogen, and phosphorus was compared to a commercial tannin-based coagulant (Tanfloc SG) and chemical ones (FeCl3 and Al2(SO4)3). Coagulant C2, at 10 mg L−1, removed 90 % of turbidity and 32 % of phosphorus, outperforming the Al-based coagulants (20 mg L−1). Moreover, CS-based coagulants reduced natural organic matter (NOM), expressed as UV absorbance at 254 nm, and dissolved organic carbon (DOC), though none significantly reduced the nitrogen levels. Toxicity tests indicated low bacterial inhibition, with C1 and C2 showing inhibition rates of 8–10 %. The sludge produced was rich in calcium (97–1500 mg g−1), magnesium (408–638 mg g−1), and potassium (347–894 mg g−1), making it suitable as a soil conditioner, though further research is necessary. Tannin-based coagulants, particularly from chestnut shells, offer a promising, sustainable solution for the post-treatment of the effluent from an aquaponics system on a fish farm while reducing organic sludge production.
{"title":"A sustainable solution for aquaculture wastewater treatment: Evaluation of tannin-based and conventional coagulants","authors":"Isabella T. Tomasi , Inês Santos , Eren Gozubuyuk , Ounísia Santos , Rui A.R. Boaventura , Cidália M.S. Botelho","doi":"10.1016/j.chemosphere.2025.144320","DOIUrl":"10.1016/j.chemosphere.2025.144320","url":null,"abstract":"<div><div>The global population faces increasing challenges in securing fresh water and food production. Aquaculture, a key source for obtaining protein, contributes significantly to environmental pollution, releasing suspended solids, organic matter, nitrogen, and phosphorus into water bodies. Some wastewater treatment methods can be expensive due to high equipment, energy, reagents, and maintenance costs, prompting the search for more sustainable alternatives. This study evaluates the performance of tannin-based coagulants from <em>Castanea sativa</em> shells (CS) for treating the recirculating water in an aquaculture plant. Two tannin-based coagulants, C1 and C2, were produced using ethanolamine (ETA) and NH<sub>4</sub>Cl, respectively. The efficiency in removing color, turbidity, organic matter, nitrogen, and phosphorus was compared to a commercial tannin-based coagulant (Tanfloc SG) and chemical ones (FeCl<sub>3</sub> and Al<sub>2</sub>(SO<sub>4</sub>)<sub>3</sub>). Coagulant C2, at 10 mg L<sup>−1</sup>, removed 90 % of turbidity and 32 % of phosphorus, outperforming the Al-based coagulants (20 mg L<sup>−1</sup>). Moreover, CS-based coagulants reduced natural organic matter (NOM), expressed as UV absorbance at 254 nm, and dissolved organic carbon (DOC), though none significantly reduced the nitrogen levels. Toxicity tests indicated low bacterial inhibition, with C1 and C2 showing inhibition rates of 8–10 %. The sludge produced was rich in calcium (97–1500 mg g<sup>−1</sup>), magnesium (408–638 mg g<sup>−1</sup>), and potassium (347–894 mg g<sup>−1</sup>), making it suitable as a soil conditioner, though further research is necessary. Tannin-based coagulants, particularly from chestnut shells, offer a promising, sustainable solution for the post-treatment of the effluent from an aquaponics system on a fish farm while reducing organic sludge production.</div></div>","PeriodicalId":276,"journal":{"name":"Chemosphere","volume":"377 ","pages":"Article 144320"},"PeriodicalIF":8.1,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143637120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-03-16DOI: 10.1016/j.chemosphere.2025.144312
Yuqing Gao , Yuzhou Jin , Yu Hu , Mingfang Li , Xuejiang Wang , Haiying Ma , Ya-nan Zhang
Composite pollution such as antibiotics and hexavalent chromium (Cr(VI)), has become a great challenge in wastewater treatment due to its severe toxicity. Herein, an advanced photoelectrocatalytic (PEC) system is constructed based on Ag/AgVO3@3D TiO2 photoelectrode to synchronously remove ofloxacin (OFL) and Cr(VI). The superior PEC performance of photoelectrode performance is attributed to the synergistic effect of heterojunction structure and the surface plasmon resonance (SPR) effect, characterized by COMSOL finite element simulation. Nearly 100 % removal of both OFL (k = 0.292 min−1) and Cr(VI) (k = 0.267 M−1 min−1) are achieved in 40 min. The promotion effect of anodic oxidation rate on cathodic reduction efficiency is studied by comparing the synchronous removal effect of other two co-existing pollutant systems (norfloxacin (NOR)-Cr(VI) and tetracycline (TC)-Cr(VI)). Main active species and intermediates are analyzed by EPR, Fukui index (f- and f+) and Methyl Phenyl Sulfoxide (PMSO) probing experiment. The stability and various influence factors are studied for practical application.
{"title":"Efficient photoelectrocatalytic simultaneous removal of antibiotic and Cr(VI) upon novel ternary plasma Ag/AgVO3@3D TiO2: Insight into the synergistic mechanism of redox","authors":"Yuqing Gao , Yuzhou Jin , Yu Hu , Mingfang Li , Xuejiang Wang , Haiying Ma , Ya-nan Zhang","doi":"10.1016/j.chemosphere.2025.144312","DOIUrl":"10.1016/j.chemosphere.2025.144312","url":null,"abstract":"<div><div>Composite pollution such as antibiotics and hexavalent chromium (Cr(VI)), has become a great challenge in wastewater treatment due to its severe toxicity. Herein, an advanced photoelectrocatalytic (PEC) system is constructed based on Ag/AgVO<sub>3</sub>@3D TiO<sub>2</sub> photoelectrode to synchronously remove ofloxacin (OFL) and Cr(VI). The superior PEC performance of photoelectrode performance is attributed to the synergistic effect of heterojunction structure and the surface plasmon resonance (SPR) effect, characterized by COMSOL finite element simulation. Nearly 100 % removal of both OFL (<em>k</em> = 0.292 min<sup>−1</sup>) and Cr(VI) (<em>k</em> = 0.267 M<sup>−1</sup> min<sup>−1</sup>) are achieved in 40 min. The promotion effect of anodic oxidation rate on cathodic reduction efficiency is studied by comparing the synchronous removal effect of other two co-existing pollutant systems (norfloxacin (NOR)-Cr(VI) and tetracycline (TC)-Cr(VI)). Main active species and intermediates are analyzed by EPR, Fukui index (<em>f</em><sup><em>-</em></sup> and <em>f</em><sup><em>+</em></sup>) and Methyl Phenyl Sulfoxide (PMSO) probing experiment. The stability and various influence factors are studied for practical application.</div></div>","PeriodicalId":276,"journal":{"name":"Chemosphere","volume":"376 ","pages":"Article 144312"},"PeriodicalIF":8.1,"publicationDate":"2025-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143632104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-03-16DOI: 10.1016/j.chemosphere.2025.144300
Haley Jo Brashears, Kayla Lea, Syed Rubaiyat Ferdous, Subham Dasgupta, Eric H. Baldwin, Lisa J. Bain
Phenolic antioxidants, such as 2,4-di-tert-butylphenol (2,4-DTBP), 2-tert-butyl phenol (2-BP), and 4-tert-butyl phenol (4-BP), are additives used in domestic water pipes, food packaging, paints, and other industrial products. As additives, they can leach from products and are frequently found in both environmental and human biological samples. Previous studies have demonstrated that 2,4-DTBP exposure can impair the differentiation of human iPS cells into somite- and sclerotome-like cells, and reduce key processes involved in osteoblast formation. Therefore, the goal of this study is to determine if 2-BP, 4-BP, 2,4-DTBP, and its metabolite 3,5-di-tert-butylcatechol (3,5-DTBC) impacts the development of cartilage and bone in vivo, using zebrafish as a model organism. Zebrafish embryos were exposed to increasing concentrations of each of the four chemicals from 1 h post fertilization (hpf) until 5 days post fertilization (dpf), and analyzed for markers of bone and cartilage development. At their highest concentrations tested, both 2-BP and 2,4-DTBP altered axial skeleton formation, with 76% and 61% of the zebrafish showing spinal curvatures, respectively. To corroborate these changes, the expression of marker transcripts were examined. 2-BP exposure reduced mRNA expression of the bone mineralization marker sparc by 1.6-fold. In contrast, 2,4-DTBP increased sparc transcript expression by 1.4-fold. All four compounds significantly upregulated sox9a, a chondrogenesis marker, between 1.4- to 5-fold. Changes in tail cartilage formation were noted using Alician blue staining, with 2,4-DTBP reducing width, length, and cartilage area of the tail, while 2-BP reduced the tail width but with increased the tail base, yielding a more straightened tail. Principle component analysis (PCA) demonstrated associations between sox9a, sparc, nrf2a, reactive oxygen species (ROS), and tail cartilage measurements, particularly in the 2,4-DTBP exposures, suggesting the involvement of nrf2a signaling in impairing cartilage formation. Overall, the study shows that each of the phenolic antioxidants differentially affects the development of bone and cartilage structures in zebrafish.
{"title":"Tert-butylphenol exposure alters cartilage and bone development in zebrafish","authors":"Haley Jo Brashears, Kayla Lea, Syed Rubaiyat Ferdous, Subham Dasgupta, Eric H. Baldwin, Lisa J. Bain","doi":"10.1016/j.chemosphere.2025.144300","DOIUrl":"10.1016/j.chemosphere.2025.144300","url":null,"abstract":"<div><div>Phenolic antioxidants, such as 2,4-di-<em>tert</em>-butylphenol (2,4-DTBP), 2-<em>tert</em>-butyl phenol (2-BP), and 4-<em>tert</em>-butyl phenol (4-BP), are additives used in domestic water pipes, food packaging, paints, and other industrial products. As additives, they can leach from products and are frequently found in both environmental and human biological samples. Previous studies have demonstrated that 2,4-DTBP exposure can impair the differentiation of human iPS cells into somite- and sclerotome-like cells, and reduce key processes involved in osteoblast formation. Therefore, the goal of this study is to determine if 2-BP, 4-BP, 2,4-DTBP, and its metabolite 3,5-di-<em>tert</em>-butylcatechol (3,5-DTBC) impacts the development of cartilage and bone <em>in vivo</em>, using zebrafish as a model organism. Zebrafish embryos were exposed to increasing concentrations of each of the four chemicals from 1 h post fertilization (hpf) until 5 days post fertilization (dpf), and analyzed for markers of bone and cartilage development. At their highest concentrations tested, both 2-BP and 2,4-DTBP altered axial skeleton formation, with 76% and 61% of the zebrafish showing spinal curvatures, respectively. To corroborate these changes, the expression of marker transcripts were examined. 2-BP exposure reduced mRNA expression of the bone mineralization marker <em>sparc</em> by 1.6-fold. In contrast, 2,4-DTBP increased <em>sparc</em> transcript expression by 1.4-fold. All four compounds significantly upregulated <em>sox9a</em>, a chondrogenesis marker, between 1.4- to 5-fold. Changes in tail cartilage formation were noted using Alician blue staining, with 2,4-DTBP reducing width, length, and cartilage area of the tail, while 2-BP reduced the tail width but with increased the tail base, yielding a more straightened tail. Principle component analysis (PCA) demonstrated associations between <em>sox9a</em>, <em>sparc</em>, <em>nrf2a</em>, reactive oxygen species (ROS), and tail cartilage measurements, particularly in the 2,4-DTBP exposures, suggesting the involvement of <em>nrf2a</em> signaling in impairing cartilage formation. Overall, the study shows that each of the phenolic antioxidants differentially affects the development of bone and cartilage structures in zebrafish.</div></div>","PeriodicalId":276,"journal":{"name":"Chemosphere","volume":"376 ","pages":"Article 144300"},"PeriodicalIF":8.1,"publicationDate":"2025-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143632105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study investigates the performance of various types of carbon membranes in reverse osmosis systems aimed at reducing salinity, nitrates, phosphates, and ammonia in aquaculture wastewater. As sustainable aquaculture practices become increasingly essential, effective treatment solutions are needed to mitigate pollution from nutrient-rich effluents. The research highlights several carbon membranes types, including carbon molecular sieves, activated carbon membranes, carbon nanotube membranes, and graphene oxide membranes, all of which demonstrate exceptional filtration capabilities due to their unique structural properties. Findings reveal that these carbon membranes can achieve removal efficiencies exceeding 90 % for critical pollutants, thereby significantly improving water quality and supporting environmental sustainability. The study also explores the development of hybrid membranes and nanocomposites, which enhance performance by combining the strengths of different materials, allowing for customized solutions tailored to the specific requirements of aquaculture wastewater treatment. Additionally, operational parameters such as pH, temperature, and feed water characteristics are crucial for maximizing membrane efficiency. The integration of real-time monitoring technologies is proposed to enable prompt adjustments to treatment processes, thereby improving system performance and reliability. Overall, this research emphasizes the importance of interdisciplinary collaboration among researchers and industry stakeholders to drive innovation in advanced filtration technologies. The findings underscore the substantial potential of carbon membranes in tackling the pressing water quality challenges faced by the aquaculture sector, ultimately contributing to the sustainability of aquatic ecosystems and ensuring compliance with environmental standards for future generations.
{"title":"Optimization of carbon membrane performance in reverse osmosis systems for reducing salinity, nitrates, phosphates, and ammonia in aquaculture wastewater","authors":"Sayyed Ali Moezzi , Saeedeh Rastgar , Monireh Faghani , Zahra Ghiasvand , Arash Javanshir Khoei","doi":"10.1016/j.chemosphere.2025.144304","DOIUrl":"10.1016/j.chemosphere.2025.144304","url":null,"abstract":"<div><div>This study investigates the performance of various types of carbon membranes in reverse osmosis systems aimed at reducing salinity, nitrates, phosphates, and ammonia in aquaculture wastewater. As sustainable aquaculture practices become increasingly essential, effective treatment solutions are needed to mitigate pollution from nutrient-rich effluents. The research highlights several carbon membranes types, including carbon molecular sieves, activated carbon membranes, carbon nanotube membranes, and graphene oxide membranes, all of which demonstrate exceptional filtration capabilities due to their unique structural properties. Findings reveal that these carbon membranes can achieve removal efficiencies exceeding 90 % for critical pollutants, thereby significantly improving water quality and supporting environmental sustainability. The study also explores the development of hybrid membranes and nanocomposites, which enhance performance by combining the strengths of different materials, allowing for customized solutions tailored to the specific requirements of aquaculture wastewater treatment. Additionally, operational parameters such as pH, temperature, and feed water characteristics are crucial for maximizing membrane efficiency. The integration of real-time monitoring technologies is proposed to enable prompt adjustments to treatment processes, thereby improving system performance and reliability. Overall, this research emphasizes the importance of interdisciplinary collaboration among researchers and industry stakeholders to drive innovation in advanced filtration technologies. The findings underscore the substantial potential of carbon membranes in tackling the pressing water quality challenges faced by the aquaculture sector, ultimately contributing to the sustainability of aquatic ecosystems and ensuring compliance with environmental standards for future generations.</div></div>","PeriodicalId":276,"journal":{"name":"Chemosphere","volume":"376 ","pages":"Article 144304"},"PeriodicalIF":8.1,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143629026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-03-15DOI: 10.1016/j.chemosphere.2025.144302
Arisbe Silva-Núñez , Xinhui Wang , Javier Donoso-Quezada , Eva C. Sonnenschein , Marie Vestergaard Lukassen , Per Juel Hansen , José González-Valdez , Thomas Ostenfeld Larsen
Prymnesium parvum is one of the main contributors to harmful algal blooms, mainly because of its ability to produce prymnesin, a toxin involved in marine specie deaths occurring in these events. At the same time, scientific works are reporting the existence of microalgae-derived extracellular vesicles in different microalgal strains, which as in other species participate in different cellular processes and intra- and intercellular communication. Now, knowing that each of the toxic Prymnesium parvum strains produce one of the three known types of prymnesin, strains PPSR01, SAG 18.97 and UTEX-2797 (that produce the C-type, B-type and A-type prymnesins, respectively) were selected to investigate the proteome of their extracellular vesicles and to elucidate their cellular functions under normal, nitrogen deficient and phosphorus deficient growth conditions. It was observed that although extracellular vesicle size and morphology did not vary significantly between strains, their proteins showed more differences among strains than among treatments. Nonetheless, it was determined that the extracellular vesicles were involved in metabolic processes, compound synthesis, gene expression and cell growth mechanisms. Additionally, significant changes among strains were found in the vesicular proteomes when these were grown in nitrogen-deficient media, whereas phosphorus deficiency only caused changes in the UTEX-2797 strain. Through metabolomic analysis, the extracellular vesicles derived from this last strain were found to transport prymnesin. Together, these findings highlight the role of microalgae-derived extracellular vesicles in the environmental stress response in P. parvum and their impact in algal blooms.
{"title":"Extracellular vesicles modulate metabolic processes in Prymnesium parvum, the causative species of algal blooms","authors":"Arisbe Silva-Núñez , Xinhui Wang , Javier Donoso-Quezada , Eva C. Sonnenschein , Marie Vestergaard Lukassen , Per Juel Hansen , José González-Valdez , Thomas Ostenfeld Larsen","doi":"10.1016/j.chemosphere.2025.144302","DOIUrl":"10.1016/j.chemosphere.2025.144302","url":null,"abstract":"<div><div><em>Prymnesium parvum</em> is one of the main contributors to harmful algal blooms, mainly because of its ability to produce prymnesin, a toxin involved in marine specie deaths occurring in these events. At the same time, scientific works are reporting the existence of microalgae-derived extracellular vesicles in different microalgal strains, which as in other species participate in different cellular processes and intra- and intercellular communication. Now, knowing that each of the toxic <em>Prymnesium parvum</em> strains produce one of the three known types of prymnesin, strains PPSR01, SAG 18.97 and UTEX-2797 (that produce the C-type, B-type and A-type prymnesins, respectively) were selected to investigate the proteome of their extracellular vesicles and to elucidate their cellular functions under normal, nitrogen deficient and phosphorus deficient growth conditions. It was observed that although extracellular vesicle size and morphology did not vary significantly between strains, their proteins showed more differences among strains than among treatments. Nonetheless, it was determined that the extracellular vesicles were involved in metabolic processes, compound synthesis, gene expression and cell growth mechanisms. Additionally, significant changes among strains were found in the vesicular proteomes when these were grown in nitrogen-deficient media, whereas phosphorus deficiency only caused changes in the UTEX-2797 strain. Through metabolomic analysis, the extracellular vesicles derived from this last strain were found to transport prymnesin. Together, these findings highlight the role of microalgae-derived extracellular vesicles in the environmental stress response in <em>P. parvum</em> and their impact in algal blooms.</div></div>","PeriodicalId":276,"journal":{"name":"Chemosphere","volume":"377 ","pages":"Article 144302"},"PeriodicalIF":8.1,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143628445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-03-14DOI: 10.1016/j.chemosphere.2025.144269
Peter Vermeiren , Sandrine Charles , Cynthia C. Muñoz
We aimed to address two common challenges for scientists working with observational data: “how to quantify the relationship between two observed (or measured) variables”, and, “how to account for censored values” (i.e., observations or measures whose value is only known to fall within a range). Quantifying the relationship between observed variables, and predicting one variable from the other (and vice versa), violates the assumption of standard regression regarding the existence of an independent, explanatory variable that is observed with no (or limited) uncertainty. To overcome this challenge, we developed and tested a Bayesian error-in-variables, EIV, regression model which accounts for uncertainty in variables orthogonally. Moreover, parameter estimation using Bayesian inference allowed the full parameter uncertainty to be propagated into probabilistic model predictions suitable for decision making. Alternative model formulations were applied to a dataset containing measured concentrations of organic pollutants in mothers and their eggs from the freshwater turtle Malaclemys terrapin and validated against an independent dataset of the turtle Chelydra serpentina. The best performing EIV model was then applied to the dataset again after censoring measurements in one or both variables. Here, independent likelihoods for both censored and uncensored data were formulated and then easily combined following the Bayesian implementation of the model. The EIV model performed well, as revealed by posterior predictive checks around 85%, and obtained comparable parameter estimates in both censored and uncensored cases. The resulting model allows scientists and decision-makers to quantitatively link variables, and make predictions from one variable to the next while accounting for uncertainties and censored data.
{"title":"Quantifying the relationship between observed variables with censored values using Bayesian error-in-variables regression","authors":"Peter Vermeiren , Sandrine Charles , Cynthia C. Muñoz","doi":"10.1016/j.chemosphere.2025.144269","DOIUrl":"10.1016/j.chemosphere.2025.144269","url":null,"abstract":"<div><div>We aimed to address two common challenges for scientists working with observational data: “how to quantify the relationship between two observed (or measured) variables”, and, “how to account for censored values” (i.e., observations or measures whose value is only known to fall within a range). Quantifying the relationship between observed variables, and predicting one variable from the other (and vice versa), violates the assumption of standard regression regarding the existence of an independent, explanatory variable that is observed with no (or limited) uncertainty. To overcome this challenge, we developed and tested a Bayesian error-in-variables, EIV, regression model which accounts for uncertainty in variables orthogonally. Moreover, parameter estimation using Bayesian inference allowed the full parameter uncertainty to be propagated into probabilistic model predictions suitable for decision making. Alternative model formulations were applied to a dataset containing measured concentrations of organic pollutants in mothers and their eggs from the freshwater turtle <em>Malaclemys terrapin</em> and validated against an independent dataset of the turtle <em>Chelydra serpentina</em>. The best performing EIV model was then applied to the dataset again after censoring measurements in one or both variables. Here, independent likelihoods for both censored and uncensored data were formulated and then easily combined following the Bayesian implementation of the model. The EIV model performed well, as revealed by posterior predictive checks around 85%, and obtained comparable parameter estimates in both censored and uncensored cases. The resulting model allows scientists and decision-makers to quantitatively link variables, and make predictions from one variable to the next while accounting for uncertainties and censored data.</div></div>","PeriodicalId":276,"journal":{"name":"Chemosphere","volume":"376 ","pages":"Article 144269"},"PeriodicalIF":8.1,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143620484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-03-14DOI: 10.1016/j.chemosphere.2025.144305
María Payá-García , Gemma M. Rodríguez-Muñiz , Marta Moreno-Torres , Erika Moro , Lucas Santos-Juanes , Ana M. Amat , Salvador Gil , José V. Castell , Virginie Lhiaubet-Vallet , Miguel A. Miranda
This work was undertaken to address the potential environmental impact of the UVA filter Uvinul® A Plus (DHHB) upon its biotransformation in humans. For this purpose, the putative human metabolite 3 was prepared by a three-step synthetic sequence involving the initial Koenigs-Knorr reaction of 2-(4-diethylamino-2-hydroxybenzoyl)benzoic acid (DHB) with acetobromo-α-d-glucuronic acid methyl ester, which afforded the corresponding peracetylated DHB-acyl glucuronide (1). Subsequent enzymatic deprotection with amano lipase A (LAS) led to the 2-(4-diethylamino-2-hydroxybenzoyl)benzoyl-β-D-glucuronide methyl ester (2). Final deprotection of compound 2 was achieved with porcine liver esterase (PLE), giving the target 2-(4-diethylamino-2-hydroxybenzoyl)benzoyl-β-D-glucuronide (3). The synthesized DHB-acyl glucuronide 3 was identical to the key phase II metabolite of DHHB in human hepatocytes. Acute toxicity of 2 and 3 was evaluated by means of the Aliivibrio fischeri bioluminescence inhibition assay, obtaining EC50 values of 22.1 mg L−1 and 105.1 mg L−1, respectively. According to the toxicity categories established by international consensus and considering that feasible concentrations of solar filters in aquatic ecosystems are several orders of magnitude lower, the glucuronide derivative of DHHB could in principle be considered as non-hazardous to the aquatic environment.
{"title":"The acyl glucuronide of 2-(4-diethylamino-2-hydroxybenzoyl)benzoic acid: Synthesis, structural assignment, occurrence as a human phase II metabolite of Uvinul® A Plus and acute aquatic toxicity","authors":"María Payá-García , Gemma M. Rodríguez-Muñiz , Marta Moreno-Torres , Erika Moro , Lucas Santos-Juanes , Ana M. Amat , Salvador Gil , José V. Castell , Virginie Lhiaubet-Vallet , Miguel A. Miranda","doi":"10.1016/j.chemosphere.2025.144305","DOIUrl":"10.1016/j.chemosphere.2025.144305","url":null,"abstract":"<div><div>This work was undertaken to address the potential environmental impact of the UVA filter Uvinul® A Plus (DHHB) upon its biotransformation in humans. For this purpose, the putative human metabolite <strong>3</strong> was prepared by a three-step synthetic sequence involving the initial Koenigs-Knorr reaction of 2-(4-diethylamino-2-hydroxybenzoyl)benzoic acid (DHB) with acetobromo-α-<span>d</span>-glucuronic acid methyl ester, which afforded the corresponding peracetylated DHB-acyl glucuronide (<strong>1</strong>). Subsequent enzymatic deprotection with amano lipase A (LAS) led to the 2-(4-diethylamino-2-hydroxybenzoyl)benzoyl-β-D-glucuronide methyl ester (<strong>2</strong>). Final deprotection of compound <strong>2</strong> was achieved with porcine liver esterase (PLE), giving the target 2-(4-diethylamino-2-hydroxybenzoyl)benzoyl-β-D-glucuronide (<strong>3</strong>). The synthesized DHB-acyl glucuronide <strong>3</strong> was identical to the key phase II metabolite of DHHB in human hepatocytes. Acute toxicity of <strong>2</strong> and <strong>3</strong> was evaluated by means of the <em>Aliivibrio fischeri</em> bioluminescence inhibition assay, obtaining EC<sub>50</sub> values of 22.1 mg L<sup>−1</sup> and 105.1 mg L<sup>−1</sup>, respectively. According to the toxicity categories established by international consensus and considering that feasible concentrations of solar filters in aquatic ecosystems are several orders of magnitude lower, the glucuronide derivative of DHHB could in principle be considered as non-hazardous to the aquatic environment.</div></div>","PeriodicalId":276,"journal":{"name":"Chemosphere","volume":"376 ","pages":"Article 144305"},"PeriodicalIF":8.1,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143620483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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