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BBX7 interacts with BBX8 to accelerate flowering in chrysanthemum. BBX7与BBX8相互作用,促进菊花开花。
Pub Date : 2023-04-01 DOI: 10.1186/s43897-023-00055-2
Yiwen Zhai, Yuqing Zhu, Qi Wang, Guohui Wang, Yao Yu, Lijun Wang, Tao Liu, Shenhui Liu, Qian Hu, Sumei Chen, Fadi Chen, Jiafu Jiang

The quantitative control of FLOWERING LOCUS T (FT) activation is important for the floral transition in flowering plants. However, the flowering regulation mechanisms in the day-neutral, summer-flowering chrysanthemum plant remain unclear. In this study, the chrysanthemum BBX7 homolog CmBBX7 was isolated and its flowering function was identified. The expression of CmBBX7 showed a diurnal rhythm and CmBBX7 exhibited higher expression levels than CmBBX8. Overexpression of CmBBX7 in transgenic chrysanthemum accelerated flowering, whereas lines transfected with a chimeric repressor (pSRDX-CmBBX7) exhibited delayed flowering. Yeast single hybridization, luciferase, electrophoretic mobility shift, and chromatin immunoprecipitation assays showed that CmBBX7 directly targets CmFTL1. In addition, we found that CmBBX7 and CmBBX8 interact to positively regulate the expression of CmFTL1 through binding to its promoter. Collectively, these results highlight CmBBX7 as a key cooperator in the BBX8-FT module to control chrysanthemum flowering.

开花位点T(FT)激活的定量控制对于开花植物的花过渡是重要的。然而,夏花菊花植株的开花调控机制尚不清楚。本研究分离了菊花BBX7同源物CmBBX7,并对其开花功能进行了鉴定。CmBBX7的表达显示出昼夜节律,并且CmBBX7表现出比CmBBX8更高的表达水平。CmBBX7在转基因菊花中的过表达加速了开花,而用嵌合阻遏物(pSRDX-CmBBX7)转染的系表现出延迟开花。酵母单杂交、萤光素酶、电泳迁移率偏移和染色质免疫沉淀分析表明,CmBBX7直接靶向CmFTL1。此外,我们发现CmBBX7和CmBBX8相互作用,通过与启动子结合,正向调节CmFTL1的表达。总之,这些结果突出了CmBBX7作为BBX8-FT模块中控制菊花开花的关键合作者。
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引用次数: 0
Salicylic acid-related ribosomal protein CaSLP improves drought and Pst.DC3000 tolerance in pepper. 水杨酸相关核糖体蛋白CaSLP提高了辣椒对干旱和Pst.DC3000的耐受性。
Pub Date : 2023-03-14 DOI: 10.1186/s43897-023-00054-3
Huafeng Zhang, Yingping Pei, Qiang He, Wang Zhu, Maira Jahangir, Saeed Ul Haq, Abid Khan, Rugang Chen

The ribosomal protein contains complex structures that belong to polypeptide glycoprotein family, which are involved in plant growth and responses to various stresses. In this study, we found that capsicum annuum 40S ribosomal protein SA-like (CaSLP) was extensively accumulated in the cell nucleus and cell membrane, and the expression level of CaSLP was up-regulated by Salicylic acid (SA) and drought treatment. Significantly fewer peppers plants could withstand drought stress after CaSLP gene knockout. The transient expression of CaSLP leads to drought tolerance in pepper, and Arabidopsis's ability to withstand drought stress was greatly improved by overexpressing the CaSLP gene. Exogenous application of SA during spraying season enhanced drought tolerance. CaSLP-knockdown pepper plants demonstrated a decreased resistance of Pseudomonas syringae PV.tomato (Pst) DC3000 (Pst.DC3000), whereas ectopic expression of CaSLP increased the Pst.DC3000 stress resistance in Arabidopsis. Yeast two-hybrid (Y2H) and bimolecular fluorescence complementation (BiFC) results showed that CaNAC035 physically interacts with CaSLP in the cell nucleus. CaNAC035 was identified as an upstream partner of the CaPR1 promoter and activated transcription. Collectively the findings demonstrated that CaSLP plays an essential role in the regulation of drought and Pst.DC3000 stress resistance.

核糖体蛋白包含属于多肽糖蛋白家族的复杂结构,参与植物生长和对各种胁迫的反应。在本研究中,我们发现辣椒40S核糖体蛋白SA样(CaSLP)在细胞核和细胞膜中广泛积累,水杨酸(SA)和干旱处理上调了CaSLP的表达水平。在CaSLP基因敲除后,能够承受干旱胁迫的辣椒植株显著减少。CaSLP的瞬时表达导致了辣椒的耐旱性,过表达CaSLP基因大大提高了拟南芥的抗旱能力。SA在喷雾季节的外源施用增强了抗旱性。在拟南芥中,CaSLP敲低的辣椒植株表现出对丁香假单胞菌PV.tomato(Pst)DC3000(Pst.DC3000)的抗性降低,而CaSLP的异位表达增加了Pst.DC3000-胁迫抗性。酵母双杂交(Y2H)和双分子荧光互补(BiFC)结果表明,CaNAC035在细胞核中与CaSLP发生物理相互作用。CaNAC035被鉴定为CaPR1启动子的上游伴侣并激活转录。总之,研究结果表明,CaSLP在干旱和Pst.DC3000胁迫抗性的调节中发挥着重要作用。
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引用次数: 0
The NAC transcription factor MdNAC4 positively regulates nitrogen deficiency-induced leaf senescence by enhancing ABA biosynthesis in apple. NAC转录因子MdNAC4通过增强苹果ABA生物合成,正向调节缺氮诱导的叶片衰老。
IF 10.6 Q1 HORTICULTURE Pub Date : 2023-03-10 DOI: 10.1186/s43897-023-00053-4
Binbin Wen, Xuehui Zhao, Xingyao Gong, Wenzhe Zhao, Mingyue Sun, Xiude Chen, Dongmei Li, Ling Li, Wei Xiao

Although it is well established that nitrogen (N) deficiency induces leaf senescence, the molecular mechanism of N deficiency-induced leaf senescence remains largely unknown. Here, we show that an abscisic acid (ABA)-responsive NAC transcription factor (TF) is involved in N deficiency-induced leaf senescence. The overexpression of MdNAC4 led to increased ABA levels in apple calli by directly activating the transcription of the ABA biosynthesis gene MdNCED2. In addition, MdNAC4 overexpression promoted N deficiency-induced leaf senescence. Further investigation showed that MdNAC4 directly bound the promoter of the senescence-associated gene (SAG) MdSAG39 and upregulated its expression. Interestingly, the function of MdNAC4 in promoting N deficiency-induced leaf senescence was enhanced in the presence of ABA. Furthermore, we identified an interaction between the ABA receptor protein MdPYL4 and the MdNAC4 protein. Moreover, MdPYL4 showed a function similar to that of MdNAC4 in ABA-mediated N deficiency-induced leaf senescence. These findings suggest that ABA plays a central role in N deficiency-induced leaf senescence and that MdPYL4 interacts with MdNAC4 to enhance the response of the latter to N deficiency, thus promoting N deficiency-induced leaf senescence. In conclusion, our results provide new insight into how MdNAC4 regulates N deficiency-induced leaf senescence.

尽管氮缺乏诱导叶片衰老已经得到了很好的证实,但氮缺乏诱导的叶片衰老的分子机制在很大程度上仍然未知。在这里,我们发现脱落酸(ABA)反应性NAC转录因子(TF)参与了缺氮诱导的叶片衰老。MdNAC4的过表达通过直接激活ABA生物合成基因MdNCED2的转录而导致苹果愈伤组织中ABA水平的增加。此外,MdNAC4过表达促进了缺氮诱导的叶片衰老。进一步的研究表明,MdNAC4直接结合衰老相关基因(SAG)MdSAG39的启动子并上调其表达。有趣的是,在ABA存在的情况下,MdNAC4在促进缺氮诱导的叶片衰老中的作用增强。此外,我们鉴定了ABA受体蛋白MdPYL4和MdNAC4蛋白之间的相互作用。此外,在ABA介导的缺氮诱导的叶片衰老中,MdPYL4表现出与MdNAC4相似的功能。这些发现表明,ABA在缺氮诱导的叶片衰老中起着核心作用,并且MdPYL4与MdNAC4相互作用以增强后者对缺氮的反应,从而促进缺氮诱导叶片衰老。总之,我们的研究结果为MdNAC4如何调节缺氮诱导的叶片衰老提供了新的见解。
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引用次数: 0
Melon (Cucumis melo) fruit-specific monoterpene synthase. 甜瓜果实特异性单萜合酶。
Pub Date : 2023-03-03 DOI: 10.1186/s43897-023-00051-6
Kathrine H Davidson, Syamkumar Sivasankara Pillai, Yukihiro Nagashima, Jashbir Singh, Rita Metrani, Kevin M Crosby, John Jifon, Bhimanagouda Patil, Seyednami Niyakan, Xiaoning Qian, Hisashi Koiwa
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引用次数: 1
Telomere-to-telomere and haplotype-resolved genome of the kiwifruit Actinidia eriantha. 猕猴桃端粒到端粒和单倍型解析基因组。
IF 10.6 Q1 HORTICULTURE Pub Date : 2023-02-17 DOI: 10.1186/s43897-023-00052-5
Yingzhen Wang, Minhui Dong, Ying Wu, Feng Zhang, Wangmei Ren, Yunzhi Lin, Qinyao Chen, Sijia Zhang, Junyang Yue, Yongsheng Liu

Actinidia eriantha is a characteristic fruit tree featuring with great potential for its abundant vitamin C and strong disease resistance. It has been used in a wide range of breeding programs and functional genomics studies. Previously published genome assemblies of A. eriantha are quite fragmented and not highly contiguous. Using multiple sequencing strategies, we get the haplotype-resolved and gap-free genomes of an elite breeding line "Midao 31" (MD), termed MDHAPA and MDHAPB. The new assemblies anchored to 29 pseudochromosome pairs with a length of 619.3 Mb and 611.7 Mb, as well as resolved 27 and 28 gap-close chromosomes in a telomere-to-telomere (T2T) manner. Based on the haplotype-resolved genome, we found that most alleles experienced purifying selection and coordinately expressed. Owing to the high continuity of assemblies, we defined the centromeric regions of A. eriantha, and identified the major repeating monomer, which is designated as Ae-CEN153. This resource lays a solid foundation for further functional genomics study and horticultural traits improvement in kiwifruit.

尼日利亚猕猴桃具有丰富的维生素C和较强的抗病性,是一种极具潜力的特色果树。它已被广泛用于育种计划和功能基因组学研究。以前发表的A.eriantha基因组组装是相当零散的,并且不是高度连续的。采用多种测序策略,我们获得了一个名为MDHAPA和MDHAPB的优良育种系“米稻31”(MD)的单倍型解析和无缺口基因组。新的组装体锚定在29对长度分别为619.3Mb和611.7Mb的假染色体上,并以端粒到端粒(T2T)的方式解析了27条和28条间隙紧密的染色体。基于单倍型解析的基因组,我们发现大多数等位基因经历了纯化选择并协同表达。由于组装体的高度连续性,我们定义了A.eriantha的着丝粒区域,并鉴定了主要的重复单体,命名为Ae-CEN153。该资源为进一步开展猕猴桃功能基因组学研究和园艺性状改良奠定了坚实的基础。
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引用次数: 0
NtMYB12 requires for competition between flavonol and (pro)anthocyanin biosynthesis in Narcissus tazetta tepals. NtMYB12需要水仙中黄酮醇和(原)花青素生物合成之间的竞争。
Pub Date : 2023-02-08 DOI: 10.1186/s43897-023-00050-7
Jingwen Yang, Xi Wu, Cristina Belen Aucapiña, Deyu Zhang, Jiazhi Huang, Ziyuan Hao, Yu Zhang, Yujun Ren, Ying Miao

The color of flowers is one of the main characteristics adopted for plants to attract pollinators to ensure the reproductive success of the plant, they are also important in their ornamental appeal in Narcissus plant. In this study, we identified a NtMYB12 locus encoding an R2R3-MYB transcription factor. Comparative transcriptome analysis of loss- and gain- of NtMYB12 tissue relative to wild-type narcissus showed NtMYB12 was mainly involved in flavonol and phenylpropanoid metabolic pathways. Biochemical evidences of dual-luciferase activity and chromatin immunoprecipitation assay supported that MYB12 directly bound to promoters of NtFLS, NtLAR, and NtDFR that were cloned by genome walking assay, and activated NtFLS and NtLAR expression but repressed NtDFR expression. More interestingly, NtMYB12 can interact with NtbHLH1 and NtWD40-1 proteins via R3 domain that were selected by transcriptome-based WGCNA and confirmed by yeast two hybrid, bimolecular fluorescence complementation and coimmunoprecipitation assay. Interaction of NtMYB12 with NtbHLH1 and NtWD40-1 forming MYB-bHLH-WD40 triplex specially activated NtDFR and NtANS expression and promoted (pro)anthocyanin accumulation, while NtMYB12 alone activated NtFLS and NtLAR expression and accumulated flavonols, but repressed NtDFR expression. These results indicated that NtMYB12 alone or NtMYB12-bHLH1-WD40-1 triplex requires for competition of metabolism fluxes between flavonol and (pro)anthocyanin biosynthesis. NtMYB12 dually functions on flavonol and proanthocyanin biogenesis via physically binding to NtFLS and NtLAR promoter activating their expression and on (pro)anthocyanin biosynthesis via NtMYB12-NtWD40-NtbHLH (MBW) triplex activating NtDFR and NtANS expression. Requirement of NtMYB12 alone or MBW complex for the competition between flavonol and anthocyanin biosynthesis results in narcissus colorized petal traits.

花的颜色是植物吸引传粉者以确保植物繁殖成功的主要特征之一,它们在水仙植物中的观赏吸引力也很重要。在这项研究中,我们鉴定了一个编码R2R3-MYB转录因子的NtMYB12基因座。NtMYB12组织相对于野生型水仙的损失和获得的比较转录组分析表明,NtMYB12主要参与黄酮醇和苯丙烷代谢途径。双荧光素酶活性和染色质免疫沉淀分析的生化证据支持MYB12直接与基因组行走分析克隆的NtFLS、NtLAR和NtDFR启动子结合,并激活NtFLS和NtLAR的表达,但抑制NtDFR的表达。更有趣的是,NtMYB12可以通过基于转录组的WGCNA选择的R3结构域与NtbHLH1和NtWD40-1蛋白相互作用,并通过酵母双杂交、双分子荧光互补和共免疫沉淀分析证实。NtMYB12与NtbHLH1和NtWD40-1的相互作用形成MYB-bHLH-WD40三链,特别激活NtDFR和NtANS的表达并促进(原)花青素的积累,而NtMYB12单独激活NtFLS和NtLAR的表达并积累黄酮醇,但抑制NtDFR的表达。这些结果表明,单独的NtMYB12或NtMYB12-bHLH1-WD40-1三链体需要黄酮醇和(原)花青素生物合成之间的代谢通量竞争。NtMYB12通过与NtFLS和NtLAR启动子物理结合激活其表达,对黄酮醇和原花青素的生物合成具有双重作用,并通过NtMYB12-NtWD40-NtbHLH(MBW)三链激活NtDFR和NtANS表达,对(原)花青素的生物合成具有双重作用。单独的NtMYB12或MBW复合物对黄酮醇和花青素生物合成之间的竞争的需求导致水仙的花瓣着色特征。
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引用次数: 4
Applications of CRISPR/Cas genome editing in economically important fruit crops: recent advances and future directions. CRISPR/Cas基因组编辑在重要经济作物中的应用:最新进展和未来方向。
IF 10.6 Q1 HORTICULTURE Pub Date : 2023-01-28 DOI: 10.1186/s43897-023-00049-0
Zhimin Ma, Lijing Ma, Junhui Zhou

Fruit crops, consist of climacteric and non-climacteric fruits, are the major sources of nutrients and fiber for human diet. Since 2013, CRISPR/Cas (Clustered Regularly Interspersed Short Palindromic Repeats and CRISPR-Associated Protein) genome editing system has been widely employed in different plants, leading to unprecedented progress in the genetic improvement of many agronomically important fruit crops. Here, we summarize latest advancements in CRISPR/Cas genome editing of fruit crops, including efforts to decipher the mechanisms behind plant development and plant immunity, We also highlight the potential challenges and improvements in the application of genome editing tools to fruit crops, including optimizing the expression of CRISPR/Cas cassette, improving the delivery efficiency of CRISPR/Cas reagents, increasing the specificity of genome editing, and optimizing the transformation and regeneration system. In addition, we propose the perspectives on the application of genome editing in crop breeding especially in fruit crops and highlight the potential challenges. It is worth noting that efforts to manipulate fruit crops with genome editing systems are urgently needed for fruit crops breeding and demonstration.

水果作物由更年期和非更年期水果组成,是人类饮食营养和纤维的主要来源。自2013年以来,CRISPR/Cas(簇状规则间隔短回文重复序列和CRISPR相关蛋白)基因组编辑系统已在不同植物中广泛应用,在许多农业重要果树的遗传改良方面取得了前所未有的进展。在这里,我们总结了果树CRISPR/Cas基因组编辑的最新进展,包括破译植物发育和植物免疫背后的机制的努力。我们还强调了基因组编辑工具在果树应用方面的潜在挑战和改进,包括优化CRISPR/Cas盒的表达,提高CRISPR/Cas试剂的递送效率,增加基因组编辑的特异性,优化转化和再生系统。此外,我们还提出了基因组编辑在作物育种特别是果树育种中的应用前景,并强调了潜在的挑战。值得注意的是,果树育种和示范迫切需要利用基因组编辑系统操纵果树。
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引用次数: 0
Warming-induced changes of broccoli head to cauliflower-like curd in Brassica oleracea are regulated by DNA methylation as revealed by methylome and transcriptome co-profiling. 甲基组和转录组共同分析显示,升温诱导的甘蓝花椰菜头向花椰菜状凝乳的变化受到DNA甲基化的调节。
Pub Date : 2022-12-22 DOI: 10.1186/s43897-022-00047-8
Zilei Yao, Lu Yuan, Ke Liu, Tingjin Wang, Bin Liu, Yan Zhao, Susheng Gan, Liping Chen

Increasingly warming temperature impacts on all aspects of growth and development in plants. Flower development is a complex process that is very sensitive to ambient temperature, and warming temperatures often lead to abnormal flower development and remarkably reduce the quality and yield of inflorescent vegetables and many other crops, which can be exemplified by Brassica oleracea cv. Green Harmony F1, a broccoli cultivar, whose floral development is ceased at inflorescence meristem (at 28 °C) or floral primordium stage (at 22 °C), forming a cauliflower-like curd (28 °C) or intermediate curd (22 °C) instead of normal broccoli head at 16 °C. However, the underlying molecular regulatory mechanisms are not well understood. Here we report that warming temperature (28 °C or 22 °C) induced hypermethylation of the genome, especially the promoter regions of such sets of genes as ribosome biogenesis-related and others, leading to the suppression of the apex-highly-expressed distinctive genes, subsequently resulting in the abnormal floral development, as revealed by methylome and transcriptome co-profiling. The regulation of warming-induced abnormal floral development in broccoli was further verified by the fact that the DNA methylation inhibitor 5-azacytidine (5-azaC) released the expression of genes from the warming temperature-induced suppression, and restored the broccoli development to normalcy at warming temperature. The research provided new approaches to breeding broccoli and other crops for growing in wider or warmer temperature zones. Graphical Abstract.

日益变暖的温度影响着植物生长发育的各个方面。花的发育是一个复杂的过程,对环境温度非常敏感,温度升高往往会导致花的发育异常,并显著降低开花蔬菜和许多其他作物的质量和产量,例如花椰菜品种Brassica oleracea cv.Green Harmony F1,其花发育在花序分生组织停止(在28 °C)或花原基期(22 °C),形成花椰菜状的凝乳(28 °C)或中间凝乳(22 °C),而不是16岁时的普通西兰花头 °C。然而,潜在的分子调控机制尚不清楚。在这里,我们报道了变暖的温度(28 °C或22 °C)诱导基因组的超甲基化,特别是核糖体生物发生相关基因和其他基因的启动子区,导致顶端高表达的独特基因被抑制,随后导致异常的花发育,如甲基组和转录组共同分析所揭示的。DNA甲基化抑制剂5-氮杂胞苷(5-azaC)释放了升温诱导的抑制基因的表达,使西兰花在升温条件下恢复正常发育,进一步证实了对升温诱导的西兰花异常花发育的调控作用。这项研究为培育西兰花和其他作物在更宽或更温暖的温度区生长提供了新的方法。图形摘要。
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引用次数: 1
Hypothesis: the subcellular senescence sequence of a mesophyll cell mirrors the cell origin and evolution. 假说:叶肉细胞的亚细胞衰老序列反映了细胞的起源和进化。
IF 10.6 Q1 HORTICULTURE Pub Date : 2022-12-06 DOI: 10.1186/s43897-022-00048-7
Su-Sheng Gan
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引用次数: 0
How a single receptor-like kinase exerts diverse roles: lessons from FERONIA. 单一受体样激酶如何发挥不同的作用:FERONIA的经验教训。
Pub Date : 2022-11-18 DOI: 10.1186/s43897-022-00046-9
Gaopeng Wang, Zhifang Zhao, Xinhang Zheng, Wenfeng Shan, Jiangbo Fan

FERONIA (FER) is a member of the Catharanthus roseus receptor-like kinase 1-like (CrRLK1L) protein subfamily, which participates in reproduction, abiotic stress, biotic stress, cell growth, hormone response, and other molecular mechanisms of plants. However, the mechanism by which a single RLK is capable of mediating multiple signals and activating multiple cellular responses remains unclear. Here, we summarize research progress revealing the spatial-temporal expression of FER, along with its co-receptors and ligands determined the function of FER signaling pathway in multiple organs. The specificity of the FER signaling pathway is proposed to operate under a four-layered mechanism: (1) Spatial-temporal expression of FER, co-receptors, and ligands specify diverse functions, (2) Specific ligands or ligand combinations trigger variable FER signaling pathways, (3) Diverse co-receptors confer diverse FER perception and response modes, and (4) Unique downstream components that modify FER signaling and responses. Moreover, the regulation mechanism of the signaling pathway- appears to depend on the interaction among the ligands, RLK receptors, co-receptors, and downstream components, which may be a general mechanism of RLKs to maintain signal specificity. This review will provide a insight into understanding the specificity determination of RLKs signaling in both model and horticultural crops.

FERONIA(FER)是长春花受体样激酶1-样(CrRLK1L)蛋白亚家族的成员,参与植物的繁殖、非生物胁迫、生物胁迫、细胞生长、激素反应和其他分子机制。然而,单个RLK能够介导多种信号并激活多种细胞反应的机制尚不清楚。在这里,我们总结了揭示FER的时空表达的研究进展,以及它的共受体和配体决定了FER信号通路在多个器官中的功能。FER信号通路的特异性被认为是在四层机制下运作的:(1)FER、共受体和配体的时空表达指定了不同的功能,(2)特异性配体或配体组合触发可变的FER信号途径,(3)不同的共受体赋予不同的FER感知和反应模式,以及(4)修改FER信号和响应的独特下游组件。此外,信号通路的调节机制似乎取决于配体、RLK受体、共受体和下游成分之间的相互作用,这可能是RLK维持信号特异性的一般机制。这篇综述将深入了解RLKs信号在模型作物和园艺作物中的特异性测定。
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引用次数: 3
期刊
Molecular Horticulture
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