Pub Date : 2021-08-24DOI: 10.15294/biosaintifika.v13i2.29496
Kristin Sangur, Alwi Smith, Meike Tomasoa
The mitotic index of the roots of pigeon pea can be the basis for determining the growth of pigeon pea. The purpose of this research was to determine the time of root cell division, to observe the mitotic phases, and to determine the mitotic index of pigeon pea root cells. The preparation of the pigeon pea was carried out for 4 days to grow the roots. The roots were cut off at 08.00, 08.15, and 08.30 WIT (Eastern Indonesian Time). The roots were cut 0.5-1cm. Carnoy’s solution was used as the fixative solution using the Squash technique. The prepared roots were then observed using an Olympus cx-22 microscope and an OptiLab camera with a magnification of 100x40. The data were descriptively analyzed to describe the images of mitotic phases and the mitotic index presentation in the root cells of pigeon pea. The results of this research showed that the cell division of the pigeon pea roots began at 08.00 WIT, which was marked by the presence of a lot of prophase. The next phases that appeared were prometaphase, metaphase, and anaphase which occurred from 08.15 to 08.30 with different numbers. The highest mitotic index occurred at 08.15, when most of the root cells underwent metaphase. This study succeeded in revealing that the optimum time for pigeon pea root cell division is 08.15 WIT. In the future, this research can help pigeon pea farmers in Southwest of Maluku to carry out vegetative reproduction which is closely related to this mitotic study.
{"title":"The Mitotic Index of Cajanus cajan from Kisar Island, in the Southwest of Maluku","authors":"Kristin Sangur, Alwi Smith, Meike Tomasoa","doi":"10.15294/biosaintifika.v13i2.29496","DOIUrl":"https://doi.org/10.15294/biosaintifika.v13i2.29496","url":null,"abstract":"The mitotic index of the roots of pigeon pea can be the basis for determining the growth of pigeon pea. The purpose of this research was to determine the time of root cell division, to observe the mitotic phases, and to determine the mitotic index of pigeon pea root cells. The preparation of the pigeon pea was carried out for 4 days to grow the roots. The roots were cut off at 08.00, 08.15, and 08.30 WIT (Eastern Indonesian Time). The roots were cut 0.5-1cm. Carnoy’s solution was used as the fixative solution using the Squash technique. The prepared roots were then observed using an Olympus cx-22 microscope and an OptiLab camera with a magnification of 100x40. The data were descriptively analyzed to describe the images of mitotic phases and the mitotic index presentation in the root cells of pigeon pea. The results of this research showed that the cell division of the pigeon pea roots began at 08.00 WIT, which was marked by the presence of a lot of prophase. The next phases that appeared were prometaphase, metaphase, and anaphase which occurred from 08.15 to 08.30 with different numbers. The highest mitotic index occurred at 08.15, when most of the root cells underwent metaphase. This study succeeded in revealing that the optimum time for pigeon pea root cell division is 08.15 WIT. In the future, this research can help pigeon pea farmers in Southwest of Maluku to carry out vegetative reproduction which is closely related to this mitotic study.","PeriodicalId":30622,"journal":{"name":"Biosaintifika Journal of Biology Biology Education","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47557049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-08-02DOI: 10.15294/biosaintifika.v13i2.30501
D. Lestari, E. R. Firdiana
Bauhinia winitii Craib (Fabaceae, Caesalpinioideae) is a woody climber which is currently included in endangered species list. B. winitii seeds are orthodox seeds in which it has hard coat morphologically. Therefore, B. winitii seeds often undergo physical dormancy which can be broken through immersion in certain liquid media. This study aimed to characterize the morphology of B. winitii seeds and determine the pre-sowing treatment method to accelerate the seeds germination of B. winitii. External morphological characterization was carried out by observing the seeds quantitative and qualitative parameters. Characterization of seeds internal morphology was conducted using a digital microscope. Physical dormancy breaking was carried out by immersion in warm and cold water for 24 hours. Morphological characterization data were analyzed descriptively, while seed germination data were analyzed quantitatively by using one-way ANOVA followed by LSD test (with confidence level of 95%). B. winitii has pod-shaped fruits with 2-6 seeds per pod, the seeds are 1.36 cm x 1 cm in size, 0.47 cm thick and weighed 0.5 g, oblong to conical in shape, smooth and shine surface, with light to dark brown in color. Seeds immersion treatment in warm water could break the physical dormancy of B. winitii seeds thus 63% of the seeds were able to germinate and it was significantly different compared to control and cold water immersion treatment. Technical to germinate B. winitii seed can be known from pre-sowing treatment. This study can be used as a reference for seed identification and germination technical of B. winitii seed.
{"title":"Morphological Characterization and Physical Dormancy of Bauhinia winitii Seed: Living Collection of Purwodadi Botanic Garden","authors":"D. Lestari, E. R. Firdiana","doi":"10.15294/biosaintifika.v13i2.30501","DOIUrl":"https://doi.org/10.15294/biosaintifika.v13i2.30501","url":null,"abstract":"Bauhinia winitii Craib (Fabaceae, Caesalpinioideae) is a woody climber which is currently included in endangered species list. B. winitii seeds are orthodox seeds in which it has hard coat morphologically. Therefore, B. winitii seeds often undergo physical dormancy which can be broken through immersion in certain liquid media. This study aimed to characterize the morphology of B. winitii seeds and determine the pre-sowing treatment method to accelerate the seeds germination of B. winitii. External morphological characterization was carried out by observing the seeds quantitative and qualitative parameters. Characterization of seeds internal morphology was conducted using a digital microscope. Physical dormancy breaking was carried out by immersion in warm and cold water for 24 hours. Morphological characterization data were analyzed descriptively, while seed germination data were analyzed quantitatively by using one-way ANOVA followed by LSD test (with confidence level of 95%). B. winitii has pod-shaped fruits with 2-6 seeds per pod, the seeds are 1.36 cm x 1 cm in size, 0.47 cm thick and weighed 0.5 g, oblong to conical in shape, smooth and shine surface, with light to dark brown in color. Seeds immersion treatment in warm water could break the physical dormancy of B. winitii seeds thus 63% of the seeds were able to germinate and it was significantly different compared to control and cold water immersion treatment. Technical to germinate B. winitii seed can be known from pre-sowing treatment. This study can be used as a reference for seed identification and germination technical of B. winitii seed.","PeriodicalId":30622,"journal":{"name":"Biosaintifika Journal of Biology Biology Education","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66971502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-04-29DOI: 10.15294/BIOSAINTIFIKA.V13I1.25954
Melycorianda Hubi Ndapamuri, M. Herawati, V. I. Meitiniarti
Sorghum stem bagasse waste is one of the materials with high cellulose content. It can be utilized in glucose production through enzymatic hydrolysis of cellulose by Trichoderma viride. This study aims to determine sorghum stem bagasse’s potential in producing glucose, assessing the time and concentration of sorghum stem bagasse in the hydrolysis process to produce glucose optimally and following SNI. Hydrolysis was carried out using a concentration of 5%, 8%, and 11% sorghum stem bagasse for 10, 15, and 20 days. The results showed that sorghum stem bagasse waste could produce glucose with an average glucose yield of 10.09% to 24.40 %. There is a tendency that increasing substrate concentration and hydrolysis time will increase the yield of liquid glucose. The treatment of 5% concentration of sorghum stem bagasse with a long hydrolysis time of 10 days can produce the highest liquid glucose, namely 24.40% with total dissolved solids of 7.40% Brix, the ash content of 0.26%, but 47.54% water content has not met SNI standards.Â
{"title":"Production of Sugar From Sweet Sorghum Stems with Hydrolysis Method Using Trichoderma viride","authors":"Melycorianda Hubi Ndapamuri, M. Herawati, V. I. Meitiniarti","doi":"10.15294/BIOSAINTIFIKA.V13I1.25954","DOIUrl":"https://doi.org/10.15294/BIOSAINTIFIKA.V13I1.25954","url":null,"abstract":"Sorghum stem bagasse waste is one of the materials with high cellulose content. It can be utilized in glucose production through enzymatic hydrolysis of cellulose by Trichoderma viride. This study aims to determine sorghum stem bagasse’s potential in producing glucose, assessing the time and concentration of sorghum stem bagasse in the hydrolysis process to produce glucose optimally and following SNI. Hydrolysis was carried out using a concentration of 5%, 8%, and 11% sorghum stem bagasse for 10, 15, and 20 days. The results showed that sorghum stem bagasse waste could produce glucose with an average glucose yield of 10.09% to 24.40 %. There is a tendency that increasing substrate concentration and hydrolysis time will increase the yield of liquid glucose. The treatment of 5% concentration of sorghum stem bagasse with a long hydrolysis time of 10 days can produce the highest liquid glucose, namely 24.40% with total dissolved solids of 7.40% Brix, the ash content of 0.26%, but 47.54% water content has not met SNI standards. ","PeriodicalId":30622,"journal":{"name":"Biosaintifika Journal of Biology Biology Education","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43911279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-04-20DOI: 10.15294/BIOSAINTIFIKA.V13I1.24794
A. Krisnawati, Nuryati Nuryati, M. Adie
One of soybean problem in Indonesia is leaf damage caused by the Spodoptera litura. The aim of the study was to identify the resistance of soybean genotypes againts army worm based on the preference index and morphophysiological characters. 150 soybean genotypes were grown under field condition from February to May 2018 in Malang (East Java, Indonesia). The preference test was conducted in the laboratory using G100H as resistant reference plant to armyworm. The result showed that the preference index was ranged from 0.09-1.82. A total of 105 genotypes showed a low preference index (resistant), 39 genotypes showed a high preference index (susceptible), and a genotype has similar preference index with the reference plant (has equal resistance with G100H). Based on the preference index and morphophysiological characters, 150 soybean genotypes can be grouped into eight clusters. A genotype from cluster VIII (Anjasmoro/Rajabasa-41) has characteristics of low leaf water content, dense leaf trichomes, and the preference index of 0.14. Those characteristics could be the attributes of the soybean genotypes that is resistant to S. litura. The Anjasmoro/Rajabasa-41 was recommended to be cultivated in Indonesian, or may be used as a gene source in the breeding program for S. litura resistance
{"title":"Assessment of Soybean Resistance to Leaf-feeding Insect, Spodoptera litura","authors":"A. Krisnawati, Nuryati Nuryati, M. Adie","doi":"10.15294/BIOSAINTIFIKA.V13I1.24794","DOIUrl":"https://doi.org/10.15294/BIOSAINTIFIKA.V13I1.24794","url":null,"abstract":"One of soybean problem in Indonesia is leaf damage caused by the Spodoptera litura. The aim of the study was to identify the resistance of soybean genotypes againts army worm based on the preference index and morphophysiological characters. 150 soybean genotypes were grown under field condition from February to May 2018 in Malang (East Java, Indonesia). The preference test was conducted in the laboratory using G100H as resistant reference plant to armyworm. The result showed that the preference index was ranged from 0.09-1.82. A total of 105 genotypes showed a low preference index (resistant), 39 genotypes showed a high preference index (susceptible), and a genotype has similar preference index with the reference plant (has equal resistance with G100H). Based on the preference index and morphophysiological characters, 150 soybean genotypes can be grouped into eight clusters. A genotype from cluster VIII (Anjasmoro/Rajabasa-41) has characteristics of low leaf water content, dense leaf trichomes, and the preference index of 0.14. Those characteristics could be the attributes of the soybean genotypes that is resistant to S. litura. The Anjasmoro/Rajabasa-41 was recommended to be cultivated in Indonesian, or may be used as a gene source in the breeding program for S. litura resistance","PeriodicalId":30622,"journal":{"name":"Biosaintifika Journal of Biology Biology Education","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45806133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-04-15DOI: 10.15294/BIOSAINTIFIKA.V13I1.28775
L. Soesanto, Clarissa Nadya Pradiptha, Endang Mugiastuti
Corn is a strategic cereal with economic value and always face the corn sheath blight in the field. An alternative safely and environmentally friendly disease control is the use of biological agent Pseudomonas fluorescens P60 that produced raw secondary metabolites. This study aimed to test the effectiveness of chitosan enriched P. fluorescens P60 raw secondary metabolites against leaf blight and to determine the effect on the growth of maize. The research was conducted at the Laboratory of Plant Protection and Experimental Farm, Faculty of Agriculture, Jenderal Soedirman University. In vitro test used a completely randomized design and in planta test used a randomized block design consisted of four treatments and six replicates. The treatments consisted of control and the secondary metabolites of P. fluorescens P60 enriched with chitosan 1, 2, and 3%. Variables observed were inhibition ability, chitinase and protease analysis qualitatively, incubation period, disease intensity, infection rate, AUDPC, crop height, crop fresh weight, and root fresh weight. The results showed that the secondary metabolites of P. fluorescens P60 enriched with chitosan 3% was effective in inhibiting the growth of Rhizoctonia solani as 63.3%, produced chitinase and protease, and could control the disease by delaying the incubation period as 79.05%, lowering the disease intensity as 68.68%, lowering the infection rate as 100%, and lowering AUDPC as 83.32%. The secondary metabolites of P. fluorescens P60 enriched with chitosan 3% was effective in improving plant growth by increasing plant fresh weight as 33.9% and root fresh weight as 43.09% compared to control. Chitosan could be used for improving effectiveness of antagonistic bacteria raw secondary metabolites. The raw secondary metabolites of bacterial antagonists could be improved their effectiveness against plant diseases by enrichment of chitosan.
{"title":"Raw Secondary Metabolites of Chitosan-enriched Pseudomonas fluorescens P60 to Control Corn Sheath Blight","authors":"L. Soesanto, Clarissa Nadya Pradiptha, Endang Mugiastuti","doi":"10.15294/BIOSAINTIFIKA.V13I1.28775","DOIUrl":"https://doi.org/10.15294/BIOSAINTIFIKA.V13I1.28775","url":null,"abstract":"Corn is a strategic cereal with economic value and always face the corn sheath blight in the field. An alternative safely and environmentally friendly disease control is the use of biological agent Pseudomonas fluorescens P60 that produced raw secondary metabolites. This study aimed to test the effectiveness of chitosan enriched P. fluorescens P60 raw secondary metabolites against leaf blight and to determine the effect on the growth of maize. The research was conducted at the Laboratory of Plant Protection and Experimental Farm, Faculty of Agriculture, Jenderal Soedirman University. In vitro test used a completely randomized design and in planta test used a randomized block design consisted of four treatments and six replicates. The treatments consisted of control and the secondary metabolites of P. fluorescens P60 enriched with chitosan 1, 2, and 3%. Variables observed were inhibition ability, chitinase and protease analysis qualitatively, incubation period, disease intensity, infection rate, AUDPC, crop height, crop fresh weight, and root fresh weight. The results showed that the secondary metabolites of P. fluorescens P60 enriched with chitosan 3% was effective in inhibiting the growth of Rhizoctonia solani as 63.3%, produced chitinase and protease, and could control the disease by delaying the incubation period as 79.05%, lowering the disease intensity as 68.68%, lowering the infection rate as 100%, and lowering AUDPC as 83.32%. The secondary metabolites of P. fluorescens P60 enriched with chitosan 3% was effective in improving plant growth by increasing plant fresh weight as 33.9% and root fresh weight as 43.09% compared to control. Chitosan could be used for improving effectiveness of antagonistic bacteria raw secondary metabolites. The raw secondary metabolites of bacterial antagonists could be improved their effectiveness against plant diseases by enrichment of chitosan.","PeriodicalId":30622,"journal":{"name":"Biosaintifika Journal of Biology Biology Education","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42043651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-04-15DOI: 10.15294/BIOSAINTIFIKA.V13I1.27665
S. Kasmiyati, E. Kristiani, M. Herawati, A. Sukmana
The medicinal plant-derived bioactive compounds have a potential for many biological activities, including antimicrobial activity. Artemisia cina is a medicinal plant from the Compositae family with the potential of having antitumor, antifungal, and antibacterial activity. This study aimed to determine the antibacterial activity and the flavonoid content of A. Cina’s ethyl acetate extract. Plants samples were extracted by ethyl acetate maceration method. Antibacterial activity was tested against Gram-negative bacteria (Escherichia coli and Pseudomonas aeruginosa) and Gram-positive bacteria (Bacillus subtilis and Staphylococcus aureus) by a disk diffusion method using 25, 50, and 100 mg/l extract concentrations. The flavonoid contents (quercetin and kaempferol) were measured using High-Performance Liquid Chromatography. The extracts of diploid and polyploid A. cina displayed some antibacterial activity, with the Gram-negative bacteria being more resistant than the Gram-positive counterpart. However, no significant difference was observed between the diploid and polyploid extracts. As for the flavonoid content, the highest quercetin content (0.5501 mg/ml) was found in the polyploid A. cina (J), while the highest kaempferol content (0.5818 mg/ml) was observed in the diploid A. cina (KJT). Although A. cina is widely grown in Indonesia, compared to other Artemisia species, A. cina has not been widely studied, especially its antibacterial potential and in related to its flavonoid content and the use of ethyl acetate as the extraction solvent.  This study reveals the potential of A. cina as a natural antibacterial agent.Â
{"title":"Antibacterial Activity and Flavonoids Content of Artemisia cina Berg. ex Poljakov Ethyl Acetate Extracts","authors":"S. Kasmiyati, E. Kristiani, M. Herawati, A. Sukmana","doi":"10.15294/BIOSAINTIFIKA.V13I1.27665","DOIUrl":"https://doi.org/10.15294/BIOSAINTIFIKA.V13I1.27665","url":null,"abstract":"The medicinal plant-derived bioactive compounds have a potential for many biological activities, including antimicrobial activity. Artemisia cina is a medicinal plant from the Compositae family with the potential of having antitumor, antifungal, and antibacterial activity. This study aimed to determine the antibacterial activity and the flavonoid content of A. Cina’s ethyl acetate extract. Plants samples were extracted by ethyl acetate maceration method. Antibacterial activity was tested against Gram-negative bacteria (Escherichia coli and Pseudomonas aeruginosa) and Gram-positive bacteria (Bacillus subtilis and Staphylococcus aureus) by a disk diffusion method using 25, 50, and 100 mg/l extract concentrations. The flavonoid contents (quercetin and kaempferol) were measured using High-Performance Liquid Chromatography. The extracts of diploid and polyploid A. cina displayed some antibacterial activity, with the Gram-negative bacteria being more resistant than the Gram-positive counterpart. However, no significant difference was observed between the diploid and polyploid extracts. As for the flavonoid content, the highest quercetin content (0.5501 mg/ml) was found in the polyploid A. cina (J), while the highest kaempferol content (0.5818 mg/ml) was observed in the diploid A. cina (KJT). Although A. cina is widely grown in Indonesia, compared to other Artemisia species, A. cina has not been widely studied, especially its antibacterial potential and in related to its flavonoid content and the use of ethyl acetate as the extraction solvent.  This study reveals the potential of A. cina as a natural antibacterial agent. ","PeriodicalId":30622,"journal":{"name":"Biosaintifika Journal of Biology Biology Education","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44877678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-04-12DOI: 10.15294/BIOSAINTIFIKA.V13I1.28141
S. Wahyuningsih, Amalia Fachrisa, Nabilatun Nisa’, Baskara Wiku Adi Kusuma, Nadia Shoukat, R. Ahmar, Na’ilah Insani Alifiyah
Sodium nitrite (NaNO2) found in vegetables, drinking water, and cured meats, can damage tissue because it is an oxidant. Plant phytochemicals such as quercetin are antioxidants. This study aimed to determine the potential of red okra pods ethanol extract (ROE) to repair kidney damage in mice (Mus musculus) induced by NaNO2. The red okra pods were extracted three times with saturated ethanol. The experiment used 36 male BALB/c mice aged 6-8 weeks and body weight of about 28 g. There are six research groups, namely, normal control, negative control (exposure to NaNO2 50 mg/kg BW), treatment of exposure to NaNO2 and administration of ROE at doses of 25, 50, 75, and 100 mg/kg BW. Sodium nitrite and ROE were given daily for 23 days by gavage. On day 24, the serum was isolated. Blood urea nitrogen (BUN) and creatinine (Cre) levels are measured to assess kidney function, as well as measuring the oxidant malondialdehyde (MDA) and the antioxidant enzyme of superoxide dismutase (SOD). The kidneys were made histological preparations and analyzed on the proximal convoluted tubule (PCT). All data were statistically analyzed (α=0.05). This study indicated that the administration of ROE at a 100 mg/kg BW dose is the most optimal in repairing damage to the PCT with increased normal cells and reduced necrosis. Besides, it degraded BUN, Cre, and MDA levels in the serum of mice exposed to NaNO2 compared to the other treatments. All doses of ROE promoted the SOD level. ROE restore kidney tissue, especially on PCT to normal. Kidney damage due to exposure to NaNO2 preservatives can be reduced by administering ROE. ROE prevents kidney damage through an increase in antioxidant enzymes. ROE can be used as a food ingredient as a source of antioxidants, thereby reducing the impact of oxidant compounds.
{"title":"Potential of Red Okra Extract (Abelmoschus esculentus L. Moench) to Restore Kidney Damage due to Sodium Nitrite","authors":"S. Wahyuningsih, Amalia Fachrisa, Nabilatun Nisa’, Baskara Wiku Adi Kusuma, Nadia Shoukat, R. Ahmar, Na’ilah Insani Alifiyah","doi":"10.15294/BIOSAINTIFIKA.V13I1.28141","DOIUrl":"https://doi.org/10.15294/BIOSAINTIFIKA.V13I1.28141","url":null,"abstract":"Sodium nitrite (NaNO2) found in vegetables, drinking water, and cured meats, can damage tissue because it is an oxidant. Plant phytochemicals such as quercetin are antioxidants. This study aimed to determine the potential of red okra pods ethanol extract (ROE) to repair kidney damage in mice (Mus musculus) induced by NaNO2. The red okra pods were extracted three times with saturated ethanol. The experiment used 36 male BALB/c mice aged 6-8 weeks and body weight of about 28 g. There are six research groups, namely, normal control, negative control (exposure to NaNO2 50 mg/kg BW), treatment of exposure to NaNO2 and administration of ROE at doses of 25, 50, 75, and 100 mg/kg BW. Sodium nitrite and ROE were given daily for 23 days by gavage. On day 24, the serum was isolated. Blood urea nitrogen (BUN) and creatinine (Cre) levels are measured to assess kidney function, as well as measuring the oxidant malondialdehyde (MDA) and the antioxidant enzyme of superoxide dismutase (SOD). The kidneys were made histological preparations and analyzed on the proximal convoluted tubule (PCT). All data were statistically analyzed (α=0.05). This study indicated that the administration of ROE at a 100 mg/kg BW dose is the most optimal in repairing damage to the PCT with increased normal cells and reduced necrosis. Besides, it degraded BUN, Cre, and MDA levels in the serum of mice exposed to NaNO2 compared to the other treatments. All doses of ROE promoted the SOD level. ROE restore kidney tissue, especially on PCT to normal. Kidney damage due to exposure to NaNO2 preservatives can be reduced by administering ROE. ROE prevents kidney damage through an increase in antioxidant enzymes. ROE can be used as a food ingredient as a source of antioxidants, thereby reducing the impact of oxidant compounds.","PeriodicalId":30622,"journal":{"name":"Biosaintifika Journal of Biology Biology Education","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46270795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-04-10DOI: 10.15294/BIOSAINTIFIKA.V13I1.27054
I. Darma, S. Sutomo, S. F. Hanum, R. Iryadi, A. Rahayu
Balinese Hindu community cannot be separated from plant in their life. The information on plant species and how to utilize them are easy to find but limited information about the potency of flowers in the Balinese Hindu community. Flowers have a meaning as sincerity, a symbol of Sang Hyang Widhi, the valour, and also have mythological values. This research aimed to determine the species of flowering plants that are often used for ceremonial and understand the meaning, the cultural conception related to conservation education. The study was carried out in four locations in Tabanan Regency (Baturiti, Penebel, Marga, and Tabanan). Data were collected using an interview with purposive sampling. Data analysis based on the value of Relative Frequency (FR) which is indicated plant most widely used. Â A total of 36 species of flowers are used in traditional Hindu religious ceremonies, of which 29 species are cultivated plants and 7 species are still wild. There are ten of plants which have high FR value, such as: Cananga odorata (FR 0.069), Impatiens balsamina (FR 0.069), Hydrangea macrophylla (FR 0.069), Plumeria acuminata (FR 0.069), Tagetes erecta (FR 0.069), Magnolia champaca (FR 0.069), Gomphrena globosa / bunga ratna (FR 0.047), Gardenia jasminoides / jempiring (FR 0.039), Nymphaea spp./ tunjung (FR 0.030), and Hibiscus rosa-sinensis L./ pucuk (FR 0.030). This research revealed the role of flower in Hindu ceremony and describe the strategic plant conservation due to culture of Balinese Hindu community. Finally, this research expected to documented local knowledge about flowers used as Hindu religious ceremonies, to provide an understanding of the meaning and conceptions of culture related to conservation education.
{"title":"Flowers and Value of Conservation in The Culture of Hindu Community in Bali","authors":"I. Darma, S. Sutomo, S. F. Hanum, R. Iryadi, A. Rahayu","doi":"10.15294/BIOSAINTIFIKA.V13I1.27054","DOIUrl":"https://doi.org/10.15294/BIOSAINTIFIKA.V13I1.27054","url":null,"abstract":"Balinese Hindu community cannot be separated from plant in their life. The information on plant species and how to utilize them are easy to find but limited information about the potency of flowers in the Balinese Hindu community. Flowers have a meaning as sincerity, a symbol of Sang Hyang Widhi, the valour, and also have mythological values. This research aimed to determine the species of flowering plants that are often used for ceremonial and understand the meaning, the cultural conception related to conservation education. The study was carried out in four locations in Tabanan Regency (Baturiti, Penebel, Marga, and Tabanan). Data were collected using an interview with purposive sampling. Data analysis based on the value of Relative Frequency (FR) which is indicated plant most widely used. Â A total of 36 species of flowers are used in traditional Hindu religious ceremonies, of which 29 species are cultivated plants and 7 species are still wild. There are ten of plants which have high FR value, such as: Cananga odorata (FR 0.069), Impatiens balsamina (FR 0.069), Hydrangea macrophylla (FR 0.069), Plumeria acuminata (FR 0.069), Tagetes erecta (FR 0.069), Magnolia champaca (FR 0.069), Gomphrena globosa / bunga ratna (FR 0.047), Gardenia jasminoides / jempiring (FR 0.039), Nymphaea spp./ tunjung (FR 0.030), and Hibiscus rosa-sinensis L./ pucuk (FR 0.030). This research revealed the role of flower in Hindu ceremony and describe the strategic plant conservation due to culture of Balinese Hindu community. Finally, this research expected to documented local knowledge about flowers used as Hindu religious ceremonies, to provide an understanding of the meaning and conceptions of culture related to conservation education.","PeriodicalId":30622,"journal":{"name":"Biosaintifika Journal of Biology Biology Education","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42076610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-04-10DOI: 10.15294/BIOSAINTIFIKA.V13I1.25264
Y. Lusiyanti, H. N. E. Surniyantoro, N. Rahajeng, Viria Agesti Suvifan, S. Purnami, L. Choridah
Individual responses to radiotherapy are often observed whether or not regimes with identical treatments were applied. Patient-related factors, the therapeutic process, and therefore the intrinsic factors of individual radiosensitivity are considered to be causing the variability of side effects. A preliminary evaluation was done on cytogenetic biomarkers found in cancer patients. The purpose of this present study was to assess the individual response of patients with cancers after radiation therapy. The sample obtained from 11 patients with different types of cancer as a case group and 12 people as a control group from a healthy volunteer. Blood samples were stimulated by an in vitro culture using phytohemagglutinin, and the cultures were assessed by using the Dicentric and Cytokinesis- Block Micronucleus (CBMN-) assay. These two methods were compared. The results showed that the overall dicentric chromosome and micronuclei in binucleate cells (MN/BNC) have a significantly higher frequency in the breast, head, and neck compared to extremity cancer. A high frequency of micronuclei in lymphocyte patients was seen after radiotherapy treatment but relatively not much higher compared to the range of micronuclei backgrounds in healthy people The CBMN is the most effective assay for evaluation of the cytogenetic studies in cancer patients because it is more radiosensitive to study individual responses. By evaluating the effects of radiotherapy based on DNA damage, the severity of radiation exposure can be studied. This study can be useful for researchers and related stakeholders in the application of radiotherapy.
{"title":"The Cytogenetic Effects on Peripheral Blood Lymphocytes in Cancer Patients After Radiation Therapy: Chromosome Aberrations and Micronuclei","authors":"Y. Lusiyanti, H. N. E. Surniyantoro, N. Rahajeng, Viria Agesti Suvifan, S. Purnami, L. Choridah","doi":"10.15294/BIOSAINTIFIKA.V13I1.25264","DOIUrl":"https://doi.org/10.15294/BIOSAINTIFIKA.V13I1.25264","url":null,"abstract":"Individual responses to radiotherapy are often observed whether or not regimes with identical treatments were applied. Patient-related factors, the therapeutic process, and therefore the intrinsic factors of individual radiosensitivity are considered to be causing the variability of side effects. A preliminary evaluation was done on cytogenetic biomarkers found in cancer patients. The purpose of this present study was to assess the individual response of patients with cancers after radiation therapy. The sample obtained from 11 patients with different types of cancer as a case group and 12 people as a control group from a healthy volunteer. Blood samples were stimulated by an in vitro culture using phytohemagglutinin, and the cultures were assessed by using the Dicentric and Cytokinesis- Block Micronucleus (CBMN-) assay. These two methods were compared. The results showed that the overall dicentric chromosome and micronuclei in binucleate cells (MN/BNC) have a significantly higher frequency in the breast, head, and neck compared to extremity cancer. A high frequency of micronuclei in lymphocyte patients was seen after radiotherapy treatment but relatively not much higher compared to the range of micronuclei backgrounds in healthy people The CBMN is the most effective assay for evaluation of the cytogenetic studies in cancer patients because it is more radiosensitive to study individual responses. By evaluating the effects of radiotherapy based on DNA damage, the severity of radiation exposure can be studied. This study can be useful for researchers and related stakeholders in the application of radiotherapy.","PeriodicalId":30622,"journal":{"name":"Biosaintifika Journal of Biology Biology Education","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41678869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-04-10DOI: 10.15294/BIOSAINTIFIKA.V13I1.27714
S. Sugiyono, Lucky Prayoga, E. Proklamasiningsih, Khavid Faozi, R. Prasetyo
Potato is an important food crop in both developed and developing countries and it is the fourth most important food crop after wheat, maize, and rice. Potato production in Indonesia has decreased in recent years, mainly due to the use of low quality tuber seed. The objective of this research was to determine the effect of plant materials, types of nutrients, and planting density on mini tuber formation, as well as to determine the best aeroponics conditions for mini tuber formation of Granola cultivar of potato. This research has been carried out experimentally using a Split-split Plot Design. The main plot was planting materials which consisted of plantlet and knol. The subplot was the type of nutrition media which consisted of Farran nutrition, Otazu nutrition, and AB-commercial mix. The sub-sub-plot was planting density which consisted of 50, 75, and 100 plants/m2. The research results showed that knol planted in aeroponics system on AB-commercial mix at a planting density of 75 plants/m2 resulted in the best mini-tuber production. In this study, for the first time three important factors for potato mini-tuber production have thoroughly been investigated in Indonesia. This finding will be used by Kledung Horticultural Seed Garden, Temanggung, for mass production of high-quality mini-tuber seed, which in turn will provide sufficient tuber seed for the farmer in its surrounding areas. By increasing the production of high-quality tuber seed, it is expected that it will contribute to the improvement of potato productivity and increase Indonesian potato production.
{"title":"The Improvement of Mini Tuber Production of Granola Potato Cultivar in Aeroponics System","authors":"S. Sugiyono, Lucky Prayoga, E. Proklamasiningsih, Khavid Faozi, R. Prasetyo","doi":"10.15294/BIOSAINTIFIKA.V13I1.27714","DOIUrl":"https://doi.org/10.15294/BIOSAINTIFIKA.V13I1.27714","url":null,"abstract":"Potato is an important food crop in both developed and developing countries and it is the fourth most important food crop after wheat, maize, and rice. Potato production in Indonesia has decreased in recent years, mainly due to the use of low quality tuber seed. The objective of this research was to determine the effect of plant materials, types of nutrients, and planting density on mini tuber formation, as well as to determine the best aeroponics conditions for mini tuber formation of Granola cultivar of potato. This research has been carried out experimentally using a Split-split Plot Design. The main plot was planting materials which consisted of plantlet and knol. The subplot was the type of nutrition media which consisted of Farran nutrition, Otazu nutrition, and AB-commercial mix. The sub-sub-plot was planting density which consisted of 50, 75, and 100 plants/m2. The research results showed that knol planted in aeroponics system on AB-commercial mix at a planting density of 75 plants/m2 resulted in the best mini-tuber production. In this study, for the first time three important factors for potato mini-tuber production have thoroughly been investigated in Indonesia. This finding will be used by Kledung Horticultural Seed Garden, Temanggung, for mass production of high-quality mini-tuber seed, which in turn will provide sufficient tuber seed for the farmer in its surrounding areas. By increasing the production of high-quality tuber seed, it is expected that it will contribute to the improvement of potato productivity and increase Indonesian potato production.","PeriodicalId":30622,"journal":{"name":"Biosaintifika Journal of Biology Biology Education","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41987411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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