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Application of Biomacromolecules in the Creation of Next-Generation Plant-Based Foods Designed for Improved Health and Sustainability. 生物大分子在创造下一代植物性食品中的应用,旨在改善健康和可持续性。
IF 5.4 2区 化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2026-02-27 DOI: 10.1021/acs.biomac.5c02661
Jaekun Ryu, David Julian McClements

Livestock animals are an important source of nutritious protein-rich foods in the human diet, including meat, milk, and eggs. However, animal welfare, environmental, sustainability, and health concerns have led to increasing interest in the development of alternatives to these animal-based products. Plant-based analogs of animal foods have emerged as a viable alternative; however, it is often challenging to accurately simulate the physicochemical, sensory, and nutritional properties of the animal products they are designed to replace. Biomacromolecules, such as proteins and polysaccharides isolated from plants, algae, and microbes, are one of the most important functional ingredients used to formulate these products. In this perspective review, we focus on the structural and functional roles of the primary biomacromolecules used in these systems: nonanimal proteins and polysaccharides. We then discuss the application of these ingredients in the formulation of milk, meat, and egg analogs, with a focus on how processing operations, proteins, and polysaccharides determine their properties. Finally, we discuss current challenges and offer insights into some of the possible future directions.

牲畜是人类饮食中营养丰富的蛋白质食物的重要来源,包括肉、奶和蛋。然而,动物福利、环境、可持续性和健康问题导致人们对开发这些动物性产品的替代品越来越感兴趣。动物性食品的植物性类似物已经成为一种可行的替代品;然而,准确地模拟它们所要取代的动物产品的物理化学、感官和营养特性往往是具有挑战性的。生物大分子,如从植物、藻类和微生物中分离的蛋白质和多糖,是用于配制这些产品的最重要的功能成分之一。在本文中,我们将重点介绍在这些系统中使用的主要生物大分子:非动物蛋白和多糖的结构和功能作用。然后,我们讨论了这些成分在牛奶、肉类和鸡蛋类似物的配方中的应用,重点是加工操作、蛋白质和多糖如何决定它们的性质。最后,我们讨论了当前的挑战,并提供了一些可能的未来方向的见解。
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引用次数: 0
Chemically Modified Alginate Grafted Penicillanic Acid Derivatives for Antibacterial Electrospun Composite Nanofibers: Electrospinnability, Drug Delivery and Cytocompatibility 化学修饰海藻酸酯接枝青霉酸衍生物的抗菌电纺复合纳米纤维:电纺性、药物传递性和细胞相容性。
IF 5.4 2区 化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2026-02-26 Epub Date: 2026-02-13 DOI: 10.1021/acs.biomac.6c00044
Yanan Bu , Jiji Fan , Shengli Lu , Xiuqiong Chen , Huiqiong Yan , Qiang Lin
Electrospinning of pure alginate is challenging due to its rigid molecular conformation and high conductivity, while conventional blends with carrier polymers severely dilute its intrinsic bioactivity. To overcome this, we developed a chemical strategy to graft hydrophobic 6-aminopenicillanic acid (6-APA) onto alginate. Among three synthetic routes, the oxidative–reductive amination-derived alginate grafted penicillanic acid derivative (RA-OSA-APA) showed dramatically enhanced flexibility, with a 10-fold lower chain entanglement concentration and a 60-fold reduced rheological crossover frequency versus pristine alginate. Blending RA-OSA-APA with poly(vinyl alcohol) (PVA) enabled the production of bead-free nanofibers with high functional biopolymer content. These composite nanofibers exhibited high encapsulation efficiency (EE) for triclosan (TCA), tunable sustained release (73–86% over 810 min), excellent cell viability (>85%), and potent antibacterial activity against Staphylococcus aureus and Escherichia coli. This work establishes oxidative–reductive amination as an effective method to concurrently solve alginate’s electrospinning processability issue and engineer advanced multifunctionality for bioactive wound dressings.
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纯海藻酸盐具有刚性分子构象和高导电性,静电纺丝具有挑战性,而与载体聚合物的常规共混严重稀释了其固有的生物活性。为了克服这个问题,我们开发了一种化学策略,将6-氨基青霉酸(6-APA)接枝到海藻酸盐上。在三种合成路线中,氧化还原氨基衍生藻酸酯接枝青霉酸衍生物(RA-OSA-APA)的柔韧性显著增强,与原始藻酸酯相比,其链缠结浓度降低了10倍,流变交叉频率降低了60倍。将RA-OSA-APA与聚乙烯醇(PVA)共混,可以生产出具有高功能生物聚合物含量的无珠纳米纤维。这些复合纳米纤维对三氯生(TCA)具有高包封率(EE)、可调缓释(73-86%)、良好的细胞活力(bb0 - 85%)和对金黄色葡萄球菌和大肠杆菌的有效抗菌活性。本研究建立了氧化还原胺化法作为解决海藻酸盐静电纺丝加工性问题的有效方法,并设计了生物活性伤口敷料的先进多功能。
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引用次数: 0
Photo-Cross-linking-Induced Morphology Change of Nonspherical Poly(trimethylene carbonate)-Based Polymer Self-Assemblies 光交联诱导非球形聚碳酸三甲酯基聚合物自组装体的形貌变化。
IF 5.4 2区 化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2026-02-23 DOI: 10.1021/acs.biomac.5c02265
Yuzhe Ma, , , Suzhen Wang, , , Zhezhe Li, , , Wenxiang Chen, , , Xiaofang Wang, , , Qiang Liu, , and , Hailong Che*, 

Nonspherical polymer self-assembled nanoparticles represent a versatile class of nanocompartments, and their anisotropic morphological features endow them with tailored functionalities, rendering them highly promising for applications in nanomedicine. However, precise control over the structure and functionality of nonspherical assemblies remains a critical challenge. In this work, we synthesized amphiphilic block copolymers composed of a hydrophilic poly(ethylene glycol) (PEG) block and a hydrophobic coumarin-functionalized poly(trimethylene carbonate) (PTMC) segment. In the presence of salts, the formulation of nonspherical assemblies (e.g., tubular polymersomes and wormlike micelles) via osmotic pressure was obtained. Upon UV light irradiation, [2+2] cycloaddition of coumarin moieties was triggered, driving a morphological transformation from tubular polymersomes to wormlike micelles. More importantly, this photoinduced structural rearrangement was exploited to construct a light-responsive cargo release system, where the morphological transition directly modulates cargo release behavior. This study not only establishes an effective strategy for the photomediated reshaping of polymer nanocompartments but also highlights the potential of photoresponsive anisotropic particles as advanced smart delivery vehicles.

非球形聚合物自组装纳米颗粒代表了一种多用途的纳米隔室,其各向异性的形态特征赋予了它们定制的功能,使它们在纳米医学中具有很大的应用前景。然而,对非球面组件的结构和功能的精确控制仍然是一个关键的挑战。在这项工作中,我们合成了由亲水聚乙二醇(PEG)嵌段和疏水香豆素功能化聚碳酸三甲酯(PTMC)段组成的两亲性嵌段共聚物。在盐的存在下,通过渗透压获得了非球形组装体(例如管状聚合体和虫状胶束)的配方。在紫外光照射下,香豆素部分的[2+2]环加成被触发,驱动从管状聚合体到虫状胶束的形态转变。更重要的是,这种光诱导结构重排被用于构建光响应的货物释放系统,其中形态转变直接调节货物释放行为。本研究不仅建立了一种有效的聚合物纳米室的光介导重塑策略,而且强调了光响应各向异性粒子作为先进智能递送载体的潜力。
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引用次数: 0
Correction to “Series of In Situ Photoinduced Polymer Graftings for Sensitive Detection of Protein Biomarkers via Cascade Amplification of Liquid Crystal Signals” 修正了“通过液晶信号级联扩增对蛋白质生物标志物进行敏感检测的一系列原位光诱导聚合物接枝”。
IF 5.4 2区 化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2026-02-14 DOI: 10.1021/acs.biomac.6c00206
Xi Wu, , , Xiaokang Ding*, , and , Fu-Jian Xu*, 
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引用次数: 0
Retraction of “3D Protein-Based Bilayer Artificial Skin for the Guided Scarless Healing of Third-Degree Burn Wounds in Vivo” “三维蛋白双层人工皮肤在体内引导三度烧伤创面无瘢痕愈合”的研究
IF 5.4 2区 化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2026-02-10 DOI: 10.1021/acs.biomac.6c00028
Mazaher Gholipourmalekabadi*, , , Alexander M. Seifalian, , , Aleksandra M. Urbanska, , , Mir Davood Omrani, , , John G. Hardy, , , Zahra Madjd, , , Seyed Mahmoud Hashemi, , , Hossein Ghanbarian*, , , Peiman Brouki Milan, , , Masoud Mozafari, , , Rui L. Reis, , , Subhas C. Kundu, , and , Ali Samadikuchaksaraei, 
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引用次数: 0
Thermosensitive Polypeptide Hydrogel Encapsulating Doxorubicin-Loaded Hollow Mesoporous Silica and CpG Nanocomplex for Chemo-Immunotherapy 热敏多肽水凝胶包封阿霉素负载中空介孔二氧化硅和CpG纳米复合物用于化学免疫治疗。
IF 5.4 2区 化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2026-02-09 Epub Date: 2026-01-14 DOI: 10.1021/acs.biomac.5c01835
Yunan Yuan , Jiaxuan Yang , Yijun Wu , Fujiang Li , Yan Rong , Huayu Tian , Chaoliang He
Chemo-immunotherapy has been an emerging synergistic strategy for melanoma treatment. However, major challenges still remain, including side effects of chemotherapeutic agents and insufficient efficacy of immunotherapy. In the present work, we designed a thermosensitive polypeptide hydrogel-based drug delivery system to achieve the codelivery of doxorubicin (DOX) and a Toll-like receptor (TLR)-9 agonist, CpG. The hydrogel system was engineered by incorporating cancer cell membrane enveloped hollow mesoporous silica loaded with DOX and the mPEG-ss-PEI/CpG nanocomplex, resulting in an enhanced therapeutic effect. Drug-loaded hydrogel system exhibited sustained drug release, enhanced immune cell activation, and induction of immunogenic cell death (ICD) of tumor cells. In vivo antitumor studies revealed that the drug-loaded hydrogel effectively inhibited tumor growth, and promoted expansion of CD8+ T cells and maturation of dendritic cells (DCs), facilitating favorable modulation of the tumor microenvironment. Hence, the developed drug-loaded hydrogel system has considerable potential as a platform for combinatorial chemo-immunotherapy in melanoma treatment.
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化学免疫疗法已成为一种新兴的黑色素瘤治疗协同策略。然而,主要的挑战仍然存在,包括化疗药物的副作用和免疫治疗的疗效不足。在本研究中,我们设计了一种基于热敏多肽水凝胶的药物递送系统,以实现阿霉素(DOX)和toll样受体(TLR)-9激动剂CpG的共递送。该水凝胶体系是通过将癌细胞膜包裹的中空介孔二氧化硅和负载DOX的mPEG-ss-PEI/CpG纳米复合物结合在一起而设计的,从而增强了治疗效果。负载药物的水凝胶系统具有持续的药物释放、增强免疫细胞激活和诱导肿瘤细胞免疫原性死亡(ICD)的特性。体内抗肿瘤研究表明,载药水凝胶可有效抑制肿瘤生长,促进CD8+ T细胞的扩增和树突状细胞(dc)的成熟,有利于肿瘤微环境的调节。因此,开发的药物负载水凝胶系统具有相当大的潜力,作为黑色素瘤联合化疗免疫治疗的平台。
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引用次数: 0
A Versatile Multidrug Coloaded Nanoplatform Integrating Photothermal-Differentiation-Chemotherapy for Breast Cancer Stemness Inhibition 一种集成光热-分化-化疗的多功能多药负载纳米平台用于乳腺癌干细胞抑制。
IF 5.4 2区 化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2026-02-09 Epub Date: 2026-01-09 DOI: 10.1021/acs.biomac.5c01675
Xiangmei Pan , Shan Zhu , Weiwei Cu , Long Jin , Jianrong Chen , Xiaoyu Zhang , Wei Zhang , Tie Wang
Cancer stem cells (CSCs) are a subpopulation of tumor cells with strong tumorigenic ability and high resistance to conventional therapeutic strategies due to the protected niche and poor drug penetration. While self-assembled nanosystems based on small-molecule self-assembly show therapeutic promise, limitations such as low targeting and unstable drug release still constrain their applications. In this study, we developed CD44-targeted RHID (ICG-DOX@RA-HA-DOX) nanocomplexes with a shell of hyaluronic acid-retinoic acid-doxorubicin (RA-HA-DOX) conjugates and a core of DOX-indocyanine green (ICG), which exhibited sustained and pH-responsive release properties. The released DOX and ICG could synergistically eliminate bulk tumors via chemotherapy and photothermal therapy. Concurrently, the released RA could promote CSC differentiation, further reducing stemness, self-renewal, and mammosphere formation, thereby enhancing the therapeutic sensitivity of CSCs to combined therapy. This integrated photothermal-differentiation-chemotherapy approach demonstrated strong antitumor efficacy both in vitro and in vivo, providing a promising nanotherapeutic strategy against CSC-driven malignancies.
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肿瘤干细胞(Cancer stem cells, CSCs)是一类肿瘤细胞亚群,具有较强的致瘤能力,由于其生态位受到保护,药物渗透性差,对常规治疗策略具有较高的耐药性。虽然基于小分子自组装的自组装纳米系统显示出治疗前景,但诸如低靶向性和药物释放不稳定等局限性仍然限制了它们的应用。在这项研究中,我们开发了靶向cd44的RHID (ICG-DOX@RA-HA-DOX)纳米复合物,其外壳为透明质酸-视黄酸-阿霉素(RA-HA-DOX)偶联物,核心为dox -吲哚菁绿(ICG),具有持续和ph响应释放特性。释放的DOX和ICG可通过化疗和光热疗法协同消除大块肿瘤。同时,释放的RA可促进CSC分化,进一步减少干细胞的干性、自我更新和乳腺球形成,从而提高CSC对联合治疗的治疗敏感性。这种综合光热-分化-化疗方法在体外和体内均显示出强大的抗肿瘤功效,为治疗csc驱动的恶性肿瘤提供了一种有前景的纳米治疗策略。
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引用次数: 0
Tuning the Viscoelasticity of Supramolecular Alginate Hydrogels via Homoternary FGG–Peptide–Cucurbit[8]uril Complexes 通过同三元fg -肽-瓜b[8] - uril配合物调节超分子海藻酸盐水凝胶的粘弹性。
IF 5.4 2区 化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2026-02-09 Epub Date: 2026-01-07 DOI: 10.1021/acs.biomac.5c01486
Nataliya Debera, Inge S. A. de Heer, Pascal Jonkheijm
Cell–ECM communication plays a critical role in the correct tissue development, disease progression, and therapeutic outcomes. The interest in controlling the mechanical properties of the ECM-mimetic systems has changed from the classical concept of elastic networks to mimic the viscoelastic behavior of the native tissue. Recently, the use of supramolecular chemistry has emerged as a promising strategy to achieve this behavior. In this work, alginate-based hydrogels were developed with a dual cross-linking system comprising dynamic cucurbit[8]­uril host–guest homoternary complexes and covalent photo-cross-linking of methacrylate groups. By adjusting the ratio of covalent to dynamic bonds, control over the stress relaxation time scale was achieved, which offers an entry to mimic the viscoelastic properties of native soft tissues. Furthermore, this hydrogel formulation was found to be noncytotoxic and promotes cell survival, attachment, and alignment.
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细胞- ecm通讯在正确的组织发育、疾病进展和治疗结果中起着关键作用。控制ecm模拟系统力学性能的兴趣已经从经典的弹性网络概念转变为模拟天然组织的粘弹性行为。最近,使用超分子化学已经成为实现这种行为的一种有前途的策略。在这项工作中,开发了海藻酸盐为基础的水凝胶,其双交联体系包括动态葫芦bbbbl主-客同源配合物和甲基丙烯酸酯基团的共价光交联。通过调整共价键与动态键的比例,实现了对应力松弛时间尺度的控制,这为模拟天然软组织的粘弹性特性提供了一个入口。此外,这种水凝胶配方被发现是无细胞毒性的,并促进细胞存活,附着和排列。
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引用次数: 0
Topical Carrier-Free Delivery of Finasteride and Peptides for Enhanced Hair Growth 局部无载体递送非那雄胺和多肽促进头发生长。
IF 5.4 2区 化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2026-02-09 Epub Date: 2026-01-06 DOI: 10.1021/acs.biomac.5c01874
Won Young Chung , Ji-eun Kim , Jae Yun Lee , Suin Kim , Woo-jin Jeong
Finasteride is widely used to treat androgenetic alopecia; however, concerns regarding systemic side effects limit its long-term use. Here, we developed a peptide-based, carrier-free topical delivery system that enhances hair growth while minimizing systemic exposure to finasteride. The system employs a skin- and cell-penetrating peptide with intrinsic anti-inflammatory activity as both the delivery vehicle and a therapeutic component. Upon coassembly with finasteride via a compositionally tuned hydrophobic block, the peptide formed well-defined nanocomplexes (NCs) that synergistically improved dermal papilla cell viability. In vivo, the NCs promoted hair regeneration to a level comparable to, or exceeding, that of 5% minoxidil, despite delivering approximately 40-fold less finasteride than the standard oral dose. Biochemical analyses confirmed accelerated transition of hair follicles from the catagen to anagen phase. This topical carrier-free strategy enhances finasteride efficacy while reducing side effects and offers a versatile platform for dermatological drug delivery.
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非那雄胺被广泛用于治疗雄激素性脱发;然而,对全身副作用的担忧限制了其长期使用。在这里,我们开发了一种基于肽的,无载体的局部给药系统,可以促进头发生长,同时最大限度地减少全身暴露于非那雄胺。该系统采用具有固有抗炎活性的皮肤和细胞穿透肽作为递送载体和治疗成分。在与非那雄胺通过组成调整的疏水块共组装后,肽形成了明确的纳米复合物(NCs),协同提高了真皮乳头细胞的活力。在体内,NCs促进头发再生的水平相当于或超过5%米诺地尔的水平,尽管非那雄胺比标准口服剂量少约40倍。生化分析证实毛囊从生长期向生长期加速过渡。这种局部无载体策略增强了非那雄胺的功效,同时减少了副作用,并为皮肤病药物递送提供了一个多功能平台。
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引用次数: 0
Design of a Mechanically Tough and Robust Lubricating Hydrogel via an Interpenetrating Hydrophilic–Hydrophobic Polymer Network 通过互穿亲疏水聚合物网络设计机械韧性和坚固的润滑水凝胶。
IF 5.4 2区 化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2026-02-09 Epub Date: 2026-01-02 DOI: 10.1021/acs.biomac.5c02455
Lei Tang, Yiling Shen, Ruixue Huang, Yue Chen, Hailan Zhou, Shuyao Liu, Hongwu Chu, Guiyin Zhou
Hydrogels show great potential for mimicking human weight-bearing tissues due to their extremely high water content and desirable behavior, including softness and elasticity. However, developing joint cartilage-mimicking hydrogels with both superior mechanical properties and stable lubrication remains challenging. This study presents a self-assembled heterostructure hydrogel approach. A mechanically robust hydrogel with sustained lubrication properties is achieved by incorporating a hydrophilic network into a hydrophobic polyethyl acrylate (PEA) matrix. Two polymer networks interweave at the microstructural level, generating water-rich and water-poor phases. Outstanding load-bearing capacity is achieved by the flexible hydrophilic polymer network efficiently dispersing impact stress into the rigid hydrophobic network. Meanwhile, a hydrated lubricating layer forms on the hydrophilic network’s surface, ensuring sustained lubrication. Moreover, the hydrophobic PEA network incorporation limits swelling in the hydrophilic network, imparting exceptionally stable antiswelling properties to the hydrogel. This study demonstrates that the heterostructure hydrogel maintains stable mechanical properties in aqueous solutions while providing lubricity, offering a novel approach to developing biomimetic materials with mechanical robustness and sustained lubricity.
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水凝胶由于其极高的含水量和理想的性能,包括柔软性和弹性,显示出模仿人体负重组织的巨大潜力。然而,开发具有优异机械性能和稳定润滑的关节软骨模拟水凝胶仍然具有挑战性。本研究提出了一种自组装异质结构水凝胶方法。通过将亲水性网络结合到疏水性聚丙烯酸酯(PEA)基质中,可以获得具有持续润滑性能的机械坚固的水凝胶。两个聚合物网络在微观结构水平上相互交织,产生富水相和贫水相。优异的承载能力是通过柔性亲水性聚合物网络有效地分散冲击应力到刚性疏水网络。同时,在亲水网络表面形成一层水合润滑层,确保持续润滑。此外,疏水PEA网络的掺入限制了亲水网络中的膨胀,赋予水凝胶异常稳定的抗膨胀性能。本研究表明,异质结构水凝胶在提供润滑性的同时在水溶液中保持稳定的力学性能,为开发具有机械稳健性和持续润滑性的仿生材料提供了新的途径。
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引用次数: 0
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Biomacromolecules
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