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Detection of Resistance Gene from Aeromonas hydrophila Isolated from Catfish Farming in Jambi 检测占碑省鲶鱼养殖中分离出的嗜水气单胞菌的抗药性基因
Pub Date : 2024-02-25 DOI: 10.29303/jppipa.v10i2.6868
Titis Wulandari, T. Wiyoko, T. W. Agrita, N. Hermana, Agustin Indrawati
Aeromonas hydrophila is one of the causative agents for Motile Aeromonas Septicemia (MAS) in catfish. MAS treatment is generally carried out by administering antibiotics. This study aimed to determine the antibiotic resistance profile of A. hydrophila and detect the presence of resistance genes. The samples are archived A. hydrophila isolates isolated from catfish ponds in the district of Muara Jambi, Bungo, Jambi Province, and Merangin total of 18 isolates. Testing the antibiotic resistance profile was carried out using the disk diffusion method against tetracycline, oxytetracycline, ampicillin, amoxicillin, ciprofloxacin, enrofloxacin, and streptomycin. The detected resistance genes were ampC, qnrA, qnrB, and qnrS. The results showed A. hydrophila isolates were resistant to tetracycline (100%), oxytetracycline (94.42%), ampicillin (77.70%), and amoxicillin (77.72%) and still sensitive to ciprofloxacin (83.32%), enrofloxacin (100 %), and streptomycin (100%). The ampC gene was found in 8 out of 14 isolates resistant to beta-lactam antibiotics (57.14%). The qnrA and qnrS genes were found in 3 out of 4 isolates resistant to quinolone antibiotics (75%), while qnrB was found in 1 isolate (25%). A. hydrophila isolated from catfish ponds in Jambi develops resistance to antibiotics and resistance genes found in isolates resistant to antibiotics.
嗜水气单胞菌是导致鲶鱼运动性嗜水气单胞菌败血症(MAS)的病原体之一。一般通过使用抗生素来治疗鲶鱼运动性气单胞菌败血症。本研究旨在确定嗜水气单胞菌的抗生素耐药性概况并检测耐药基因的存在。样本是从占碑省Muara Jambi、Bungo和Merangin地区的鲶鱼池塘中分离出的蚜茧蜂蝶菌,共18个。采用磁盘扩散法对四环素、土霉素、氨苄西林、阿莫西林、环丙沙星、恩诺沙星和链霉素进行了抗药性测试。检测到的抗药性基因有 ampC、qnrA、qnrB 和 qnrS。结果显示,水飞蓟马分离株对四环素(100%)、土霉素(94.42%)、氨苄西林(77.70%)和阿莫西林(77.72%)耐药,对环丙沙星(83.32%)、恩诺沙星(100%)和链霉素(100%)仍然敏感。在 14 个对 beta-内酰胺类抗生素耐药的分离物中,有 8 个(57.14%)发现了 ampC 基因。对喹诺酮类抗生素耐药的 4 个分离物中有 3 个(75%)发现了 qnrA 和 qnrS 基因,而 1 个分离物(25%)发现了 qnrB 基因。从占碑省的鲶鱼池塘中分离出的嗜水蝇会对抗生素产生抗药性,在对抗生素产生抗药性的分离物中发现了抗药性基因。
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引用次数: 0
Use of STEM Integrated Crosscutting Concepts Science Module on Science Curiosity of Junior High School STEM 综合横向概念科学模块对初中生科学好奇心的影响
Pub Date : 2024-02-25 DOI: 10.29303/jppipa.v10i2.5073
Fairuz Zakirah, Asri Widowati
One of the goals of learning science is to help students develop their science curiosity. Students' curiosity is a personality trait motivated by the desire to find a solution or fill a specific knowledge gap. Using science modules in science is expected to be a solution to stimulate student’s science curiosity. The purpose of this study was to determine the curiosity level of class VII students at SMP Negeri 1 Yogyakarta, especially on the temperature and heat topic. Type of this research is descriptive qualitative. Data analysis used the N-gain test to determine the increase in student’s science curiosity. The subjects in this study were students of class VII G SMP Negeri 1 Yogyakarta, who collected 32 students using purposive sampling technique. Data collection techniques consist of questionnaires and the data collection used in the form of pretest and posttest. Based on the data analysis there is difference in the pretest and posttest results with the average N-gain obtained is 0.69, which is in the medium category. It can be concluded that the use of STEM integrated Crosscutting Concepts can improve students’ science curiosity. This is evidenced by the difference in pretest and posttest, also the N-gain score which is in medium category.
学习科学的目标之一是帮助学生培养对科学的好奇心。学生的好奇心是一种个性特征,其动机是希望找到解决方案或填补特定的知识空白。在科学课中使用科学模块有望成为激发学生科学好奇心的一种解决方案。本研究的目的是确定日惹国立第一中学七年级学生的好奇心水平,尤其是对温度和热量主题的好奇心水平。研究类型为描述性定性研究。数据分析采用 N-增益测试来确定学生科学好奇心的增长情况。本研究的对象是日惹国立日惹第一中学七年级 G 班的学生,使用目的性抽样技术收集了 32 名学生。数据收集技术包括问卷调查,数据收集采用前测和后测的形式。根据数据分析,前测和后测结果存在差异,平均 N-gain为 0.69,属于中等水平。可以得出结论,使用 STEM 综合横切概念可以提高学生的科学好奇心。前测和后测的差异以及 N-gain(N-增益)得分属于中等水平,都证明了这一点。
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引用次数: 0
Application of Learning Media in Improving Higher Order Thinking Skills in Electronics Teaching Materials 在电子教材中应用学习媒体提高高阶思维能力
Pub Date : 2024-02-25 DOI: 10.29303/jppipa.v10i2.6256
M. Silitonga, M. Solihin, Muhammad Isnaini, H. Hutahaean
This research aims to create a learning multimedia application that can be used by students in learning. The method used is development research from Jan Van De Akker with formative evaluation from Martin Tessmer. The research stage consists of four stages, namely the preliminary research stage, prototype, summative evaluation, and systematic reflection and documentation. Based on user assessment, the learning multimedia is practical to use with a practicality level of 83% and is included in the good category. The effectiveness of multimedia at the summative evaluation stage is based on the post-test results. The results of the student posttest stated that 9 out of 12 students scored ≥75, meaning that 75% of the participants passed and this multimedia is effective. The results showed that the learning multimedia application can improve the higher-order thinking skills of students.
本研究旨在创建一个可供学生学习使用的学习多媒体应用程序。采用的方法是 Jan Van De Akker 的开发研究和 Martin Tessmer 的形成性评价。研究阶段包括四个阶段,即初步研究阶段、原型、总结性评价以及系统反思和记录。根据用户评估,学习多媒体的实用性为 83%,属于良好类。在终结性评价阶段,多媒体的有效性基于后测结果。学生后测结果表明,12 名学生中有 9 人得分≥75 分,这意味着 75% 的学员通过了测试,该多媒体是有效的。结果表明,学习多媒体应用可以提高学生的高阶思维能力。
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引用次数: 0
Effectiveness of Character-Based Comic Media on Environmental Conservation Sub-Material Learning Outcomes 人物漫画媒体对环境保护副教材学习成果的影响
Pub Date : 2024-02-25 DOI: 10.29303/jppipa.v10i2.6148
Lilis Indriyani, Kurnia Ningsih, Anisyah Yuniarti
The utilization of learning media is one of the alternatives to increase knowledge for students, especially biology learning. In addition, student character can be improved through media in which there is character insertion. Thus, an innovative learning media is needed to improve knowledge and character through character-based comics on environmental conservation submaterial. This research method is a quantitative experiment, with a pretest-posttest control group research design. The sample consisted of 28 control class students and 30 experimental class students. The instruments were 20 multiple choice questions and 22 character questionnaire statements. Data analysis techniques include knowledge assessment measured using the Independent samples t-test formula. The results of data analysis showed that the knowledge of experimental class students increased significantly, the learning outcomes in the experimental class showed a higher average of 86.67 while the control class was 74.46, the average difference in learning outcomes between experimental and control classes was evidenced by the independent samples t-test test value of 0.00 <0.05, and student character showed a percentage of 81.44% in the excellent category. It is concluded that character-based comic media on environmental conservation submaterial can help improve cognitive learning outcomes and student character.
利用学习媒体是增加学生知识,特别是生物学习知识的替代方法之一。此外,学生的品格也可以通过插入人物形象的媒体得到提高。因此,需要一种创新的学习媒体,通过基于人物的环境保护子材料漫画来提高学生的知识和品格。本研究方法为定量实验,采用前测-后测对照组研究设计。样本包括 28 名对照班学生和 30 名实验班学生。研究工具为 20 道选择题和 22 份人物问卷陈述。数据分析技术包括使用独立样本 t 检验公式测量知识评估。数据分析结果表明,实验班学生的知识水平明显提高,实验班的学习成果平均值为 86.67,而对照班为 74.46,实验班与对照班的学习成果平均值差异由独立样本 t 检验的检验值 0.00 <0.05 得到证明,学生品德表现为优秀类的比例为 81.44%。结论是,基于人物形象的环境保护子材料漫画媒体有助于提高认知学习成果和学生品格。
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引用次数: 0
Encyclopedia of Flora on Curiak Island to Improve Critical Thinking Skills of Science Education Students 库里亚克岛植物百科全书》提高科学教育专业学生的批判性思维能力
Pub Date : 2024-02-25 DOI: 10.29303/jppipa.v10i2.6128
Mella Mutika Sari, R. Yulinda, Desy Purwasih
This research aims to produce a flora encyclopedia product on Curiak Island that is suitable for improving critical thinking skills.  Type of Educational Design Research (EDR) research using Tessmer formative evaluation design. The formative evaluation used consists of four stages, namely the self-evaluation stage, expert review, one-to-one evaluation, and small group evaluation. Collecting product validation data using an encyclopedia assessment instrument. Data regarding validity is collected through an encyclopedia validation sheet by giving a score of 1, 2, 3, or 4 (1 = not good, 2 = quite good, 3 = good, and 4 = very good). Critical thinking assessment use essay with four indicators. The findings show that the product developed is feasible in terms of material, media and language. The test results show that there is a significant difference in students' critical thinking abilities before and after learning. Apart from that, the analysis indicator in critical thinking skills obtained the highest score in the medium category. These findings illustrate that the effectiveness of product expectations is effective in improving students' critical thinking abilities.
本研究旨在制作适合提高批判性思维能力的库里亚克岛植物百科全书产品。 采用泰瑟默形成性评价设计的教育设计研究(EDR)研究类型。所采用的形成性评价包括四个阶段,即自我评价阶段、专家评审、一对一评价和小组评价。使用百科全书评估工具收集产品验证数据。通过百科全书评估表收集有关有效性的数据,给出 1、2、3 或 4 分(1 = 不好,2 = 相当好,3 = 好,4 = 非常好)。批判性思维评估使用四项指标的论文。结果表明,所开发的产品在材料、媒体和语言方面都是可行的。测试结果表明,学生的批判性思维能力在学习前后有显著差异。此外,批判性思维能力的分析指标在中等类别中得分最高。这些结果说明,产品预期的有效性能有效提高学生的批判性思维能力。
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引用次数: 0
Influence of the STEM-Based Engineering Design Process Model on High School Students' Creative and Critical Thinking Abilities 基于 STEM 的工程设计过程模型对高中生创造性思维和批判性思维能力的影响
Pub Date : 2024-02-25 DOI: 10.29303/jppipa.v10i2.4765
Wulan Safitri, S. Suyanto, Wanda Agus Prasetya
STEM-based Engineering Design Process learning model as a learning innovation to improve creative and critical thinking skills. This research aims to determine the influence of the STEM-based Engineering Design Process learning model on creative and critical thinking skills in a multivariate manner, as well as the correlation between students' creative and critical thinking skills on the subject of environmental pollution. The design of this research is Quasi Experimental with a pretest-posttest control group design. The treatment given to the experimental class was learning using the STEM-based Engineering Design Process model, while the control class used the Discovery Learning model with a scientific approach. The results of this research show that there is an influence of the STEM-based Engineering Design Process model on the ability to think creatively and critically in a multivariate manner with a value of sig. in Hotelling's trace from the MANOVA test 0.000 ‹ 0.05, and there is a correlation between creative and critical thinking abilities and the sig value. from the pearson test ‹ 0.000 ‹ 0.05. In conclusion, the STEM-based Engineering Design Process model is able to improve creative and critical thinking abilities, and there is a correlation between creative and critical thinking abilities.
基于 STEM 的工程设计过程学习模式是一种提高创造性和批判性思维能力的学习创新。本研究旨在以多元方式确定基于 STEM 的工程设计过程学习模式对创造性思维和批判性思维能力的影响,以及学生在环境污染问题上的创造性思维和批判性思维能力之间的相关性。本研究采用前测-后测对照组的准实验设计。实验班的处理方法是使用基于 STEM 的工程设计过程模式进行学习,而对照班则使用科学方法的发现学习模式。研究结果表明,基于 STEM 的工程设计过程模式对创造性思维和批判性思维能力有多元影响, MANOVA 检验的 Hotelling's trace 的 sig 值为 0.000 ' 0.05,创造性思维和批判性思维能力之间存在相关性,pearson 检验的 sig 值为 0.000 ' 0.05。总之,基于 STEM 的工程设计过程模式能够提高创造性思维能力和批判性思维能力,并且创造性思维能力和批判性思维能力之间存在相关性。
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引用次数: 0
Application of Chitosan from Litopenaeus vannamei and Baglog Waste from Pleurotus ostreatus for Decolorizing Batik Wastewater 应用壳聚糖(从万年青中提取)和袋藻废料(从褐藻中提取)对蜡染废水进行脱色处理
Pub Date : 2024-02-25 DOI: 10.29303/jppipa.v10i2.5859
Swastika Oktavia, Siti Rohmah, Cory Novi
The batik sector generates wastewater containing pigment residue, posing a significant environmental threat if not managed effectively. L. vannamei shells yield chitin, convertible to chitosan for water treatment. Baglogs, comprising mycelium and cellulose, serve as a viable adsorbent. The purpose of this study was to learn about the use shell of L. vannamei and P. ostreatus baglog waste with different lengths of time to decolorization batik industry waste water. The method for testing involves deproteinization with NaOH 1M, demineralization with HCl 2M and deasetylation with NaOH 60%. Chitosan L. vannamei and baglog P. ostreatus (2:25 g/g) waste were put into a container containing 500 mL of batik wastewater with colour content with variations of 0, 12, 24, 48, 72, 96, and 120 hours. Further, the capacity of adsorption chitosan and baglog waste are determined using a UV-Vis spectrophotometer during the decolorization process in batik industry waste water using shell of L. vannamei and P. ostreatus baglog waste are 96 hours with a decolorization rate of 90.05%.
蜡染行业会产生含有颜料残留物的废水,如果不加以有效管理,会对环境造成严重威胁。凡纳米贝壳可产生甲壳素,可转化为壳聚糖用于水处理。由菌丝体和纤维素组成的袋涝是一种可行的吸附剂。本研究的目的是了解使用不同时间长度的凡纳米贝壳和奥斯特罗贝壳废料来脱色蜡染工业废水的情况。测试方法包括用 NaOH 1M 进行脱蛋白,用 HCl 2M 进行脱矿物质,用 NaOH 60% 进行脱乙酰基。将壳聚糖 L. vannamei 和 baglog P. ostreatus(2:25 g/g)废料放入装有 500 毫升蜡染废水的容器中,废水中的色素含量在 0、12、24、48、72、96 和 120 小时内变化。此外,使用紫外可见分光光度计测定了壳聚糖和袋藻废料在蜡染工业废水脱色过程中的吸附能力,使用凡纳米贝壳和袋藻废料脱色 96 小时,脱色率为 90.05%。
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引用次数: 0
Hematological Analysis of Red Bader Fish (Barbonymus altus) as a Biomarker in Assessing Pollution Status in Brantas River, Surabaya, East Java 作为评估东爪哇泗水 Brantas 河污染状况的生物标志物的红獾鱼(Barbonymus altus)血液学分析
Pub Date : 2024-02-25 DOI: 10.29303/jppipa.v10i2.5659
Arief Farhan Kurniawan Anasiru, A. Hertika, U. Yanuhar
The Brantas River has many functions for both humans and the aquatic organisms in it. The type of fish that is often found in the Brantas River in Surabaya is the red bader fish (Barbonymus altus). Hematology is a sensitive parameter and can be used as a marker in assessing water conditions. The aim of this research is to analyze the hematological profile of red bader fish (Barbonymus altus) to assess the condition of the Brantas River, Surabaya. The research was carried out at 3 locations on the Brantas River in Surabaya with 3 sampling times with an interval of 1 month starting in January – March 2022. The parameters observed were water quality (temperature, TSS, TDS, pH, DO, BOD, ammonia and heavy metals Hg) and hematological parameters (erythrocytes, hemoglobin, hematocrit, leukocytes and micronuclei). Analysis of the relationship between water quality parameters and hematology using the Correspondence Correlation Analysis (CCA) method. The results show that there are water quality parameters that do not meet quality standards and there are abnormal hematological parameters. CCA analysis shows that there is a correlation between water quality and hematological parameters with moderate concentrations (erythrocytes, hemoglobin and hematocrit) and strong concentrations (leukocytes and micronuclei).
布兰达斯河对人类和河中的水生生物都有许多功能。泗水 Brantas 河中常见的鱼类是红獾鱼(Barbonymus altus)。血液学是一种敏感参数,可用作评估水质状况的标志。本研究旨在分析红獾鱼(Barbonymus altus)的血液学特征,以评估泗水布兰达斯河的状况。研究在泗水布兰塔斯河的 3 个地点进行,从 2022 年 1 月到 3 月,共采样 3 次,每次间隔 1 个月。观察参数包括水质(温度、总悬浮固体、总溶解氧、pH 值、溶解氧、生化需氧量、氨氮和重金属汞)和血液学参数(红细胞、血红蛋白、血细胞比容、白细胞和微核)。采用对应相关分析法(CCA)分析水质参数与血液学之间的关系。结果显示,存在水质参数不达标和血液学参数异常的情况。CCA 分析表明,水质与血液学参数之间存在相关性,浓度适中(红细胞、血红蛋白和血细胞比容),浓度较高(白细胞和微核)。
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引用次数: 0
Development of Android-Based Interactive Mobile Learning with Guided Inquiry Models to Improve Higher Order Thinking Skills on Light Wave Material 开发基于 Android 的交互式移动学习与引导探究模式,以提高光波材料的高阶思维能力
Pub Date : 2024-02-25 DOI: 10.29303/jppipa.v10i2.4869
J. Penelitian, Pendidikan Ipa, Siti Meisaroh, Suparno
This research aims to produce Android-based interactive mobile learning with a guided inquiry model of light wave material that is able to improve students' HOTS abilities. This research is a 4D model type research. The research was conducted at MAN 1 Ponorogo, MAN 2 Ponorogo, and SMAN 1 Badegan. The research subjects were 149 class XI MIPA students who gave a pretest-posttest. Student pretest-posttest data were analyzed using 1-way ANOVA to determine the differences in students' HOTS skills in the control and experimental classes. Based on the 1-way ANOVA test, it was found that there were differences in students' HOTS abilities in the two classes. Classes that use Android-based interactive car learning get higher results compared to classes that don't use Android-based interactive car learning.
本研究旨在制作基于安卓系统的交互式移动学习,其光波材料的引导探究模型能够提高学生的 HOTS 能力。本研究是一项 4D 模型型研究。研究在波诺罗戈第一城域网(MAN 1 Ponorogo)、波诺罗戈第二城域网(MAN 2 Ponorogo)和巴德干第一城域网(SMAN 1 Badegan)进行。研究对象是 149 名 MIPA 第十一班学生,他们进行了前测-后测。学生的前测-后测数据通过单因素方差分析来确定对照班和实验班学生 HOTS 技能的差异。根据单因素方差分析,发现两个班级学生的 HOTS 能力存在差异。使用安卓交互式学车系统的班级与不使用安卓交互式学车系统的班级相比,获得了更高的成绩。
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引用次数: 0
Students’ and Teachers’ Perception on the Development of Local Wisdom Module to Increase Student Learning Outcome 师生对开发乡土智慧模块以提高学生学习成果的看法
Pub Date : 2024-02-25 DOI: 10.29303/jppipa.v10i2.5063
J. Penelitian, Pendidikan Ipa, Mellawen, F. Zannah, Tazkiyatunnafs Elhawwa
In this study we focus to analyze the teacher and student perception about the development of the local wisdom module, especially to increase learning outcome. The research method is descriptive and qualitative by used surveys to collect data. Data collection was carried out by distributing questionnaires to 2 elementary school teacher and to 20 student 5th grade students, SDN 3 Langkai in Palangkaraya city, Central Kalimantan. The collected data were analyzed using descriptive analysis. The results of this research showed that teachers have never developed their own teaching materials based on local potential in Central Kalimantan, while students are of the opinion that the use of teaching materials based on local potential will make learning activities more interesting. This research concluded that the development of local wisdom module especially about batang garing very important thing to do based on teacher and student perception and important to continue in the next research to develop the module.
在本研究中,我们重点分析了教师和学生对开发本地智慧模块的看法,尤其是对提高学习效果的看法。研究采用描述性和定性研究方法,通过调查收集数据。数据收集是通过向中加里曼丹省巴郎卡拉亚市 SDN 3 Langkai 的 2 名小学教师和 20 名五年级学生发放问卷的方式进行的。收集到的数据采用描述性分析方法进行分析。研究结果表明,在中加里曼丹,教师从未根据当地潜力开发过自己的教材,而学生则认为使用根据当地潜力开发的教材会使学习活动更有趣。本研究得出结论,根据教师和学生的看法,开发地方智慧模块,尤其是关于巴塘加林的模块非常重要,在下一次研究中继续开发该模块非常重要。
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引用次数: 0
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Jurnal Penelitian Pendidikan IPA
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