cAMP responsive element modulator (CREM) is one of the most important transcription factors expressed in male testes which connect the extra cellular signals to gene regulation. CREM expression switches from a repressor to an activator in testes during spermatogenesis. Any alteration in this switch leads to different testicular patterns. Methods: Total RNA was extracted from Eighty eight testicular biopsy and analyzed for CREM expression profiling. Normal spermatogenesis showed expression of CREM2and gamma repressor, the same finding was detected in Hypospermatogenesis group but with another longer repressor belong to alpha repressor type, 95% of Round spermatid maturation arrest group showed two repressor patterns, and 5% showed two activator transcripts of CREM2 activator. Sertoli cells only group didn’t show any activator transcripts of CREM. In conclusion studying the mechanisms of interaction between CREM repressors and activators in the normal and impaired spermatogenesis may lead to better understanding of genetic control of the spermatogenesis and consequently to better treatment options.
{"title":"EXPRESSION PROFILING OF CREM GENE IN TESTIS WITH NORMAL AND IMPAIRED SPERMATOGENESIS IN EGYPTIAN MALES","authors":"M. E. Hamshary, A. Issa, M. Khalifa, K. Shaeer","doi":"10.21608/EJGC.2014.9941","DOIUrl":"https://doi.org/10.21608/EJGC.2014.9941","url":null,"abstract":"cAMP responsive element modulator (CREM) is one of the most important transcription factors expressed in male testes which connect the extra cellular signals to gene regulation. CREM expression switches from a repressor to an activator in testes during spermatogenesis. Any alteration in this switch leads to different testicular patterns. Methods: Total RNA was extracted from Eighty eight testicular biopsy and analyzed for CREM expression profiling. Normal spermatogenesis showed expression of CREM2and gamma repressor, the same finding was detected in Hypospermatogenesis group but with another longer repressor belong to alpha repressor type, 95% of Round spermatid maturation arrest group showed two repressor patterns, and 5% showed two activator transcripts of CREM2 activator. Sertoli cells only group didn’t show any activator transcripts of CREM. In conclusion studying the mechanisms of interaction between CREM repressors and activators in the normal and impaired spermatogenesis may lead to better understanding of genetic control of the spermatogenesis and consequently to better treatment options.","PeriodicalId":31811,"journal":{"name":"Egyptian Journal of Genetics and Cytology","volume":"43 1","pages":"199-205"},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68480353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The Allium cepa assay is an efficient test for chemical screening and in situ monitoring for genotoxicity of environmental contaminants. This test has been used widely to study genotoxicity of many chemicals pollutions revealing that these compounds can induce chromosomal aberrations in root meristems of Allium cepa. In this study, we aimed to determine genotoxic effects of some textile azo dyes by using the Allium cepa chromosome aberrations test and random amplification of polymorphic DNA (RAPD) analyses. The onion (Allium cepa L.) roots were exposed to different concentrations of three textile azo dyes. The mitotic index of samples exposed to EC50 (500 μg/ml) of selected azo dyes for RLB, RN and SGL was 10.8, 10.3 and 8.8, respectively. The results indicated that the root length of Allium cepa reduced with an increasing azo dye concentration. A random amplification of polymorphic DNA (RAPD) analysis from the extracted DNA was carried out using ten 10-base pair random primers. Ten primers produced 54 bands between 100-1600 base pairs in gel electrophoresis. The number of disappearing bands in profiles was differenced from one to five bands of azo dyes treatment compared to total bands in control and new bands were appeared in treatments. Obtained results from this study revealed that the total chromosomal aberrations and RAPD profiles were performed as useful tool for detection and biomarker assays for the evaluation of genotoxic effects on textile azo dyes polluted plants.
{"title":"CYTOGENOTOXICITY EVALUATION OF WATER CONTAMI- NATED WITH SOME TEXTILE AZO DYES USING RAPD MARK- ERS AND CHROMOSOMAL ABERRATIONS OF ONION (Allium cepa) ROOT CELLS","authors":"G. M. Hassan, A. Yassein","doi":"10.21608/EJGC.2014.9932","DOIUrl":"https://doi.org/10.21608/EJGC.2014.9932","url":null,"abstract":"The Allium cepa assay is an efficient test for chemical screening and in situ monitoring for genotoxicity of environmental contaminants. This test has been used widely to study genotoxicity of many chemicals pollutions revealing that these compounds can induce chromosomal aberrations in root meristems of Allium cepa. In this study, we aimed to determine genotoxic effects of some textile azo dyes by using the Allium cepa chromosome aberrations test and random amplification of polymorphic DNA (RAPD) analyses. The onion (Allium cepa L.) roots were exposed to different concentrations of three textile azo dyes. The mitotic index of samples exposed to EC50 (500 μg/ml) of selected azo dyes for RLB, RN and SGL was 10.8, 10.3 and 8.8, respectively. The results indicated that the root length of Allium cepa reduced with an increasing azo dye concentration. A random amplification of polymorphic DNA (RAPD) analysis from the extracted DNA was carried out using ten 10-base pair random primers. Ten primers produced 54 bands between 100-1600 base pairs in gel electrophoresis. The number of disappearing bands in profiles was differenced from one to five bands of azo dyes treatment compared to total bands in control and new bands were appeared in treatments. Obtained results from this study revealed that the total chromosomal aberrations and RAPD profiles were performed as useful tool for detection and biomarker assays for the evaluation of genotoxic effects on textile azo dyes polluted plants.","PeriodicalId":31811,"journal":{"name":"Egyptian Journal of Genetics and Cytology","volume":"43 1","pages":"39-57"},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68479593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Water is important for all the living things but nowadays, it has been polluted with inorganic contaminants which are discharged from industries. This study was carried out to evaluate the potential of Rhizobium leguminosarum, Azotobacter chrocoocum, Serratia marcescns and Bacillus circulans to survive in effluent obtained from Alexandria and Sadat City and the ability of these bacteria to remove metals from effluent. Samples of effluent were assessed for various physicochemical parameters like pH, Electrical conductivity (EC), concentration of salts, osmotic pressure, biological oxygen demand (BOD) (35 and 65), chemical oxygen demand (COD) (94 and 135) in Alexandria and Sadat City, respectively. The pH was 7.58 and 7.78, respectively compared with tap water (control) pH from Sadat City and Alexandria 7.4-7.2, respectively. It was found that different the concentrations of media containing effluent obtained from Alexandria and Sadat City having negative effect on bacterial growth and the more effective of Sadat City effluent, except Bacillus circulans had the same trend. The examined bacteria were capable of removing metals from different sources of effluent (Alexandria and Sadat City), Cu++ ions was highest removed by Rhizobium leguminosarum (44% and 39%), Azotobacter chrocoocum (42% and 50%), Serratia marcescns (50% and 50%) and Bacillus circulans (36% and 46%, respectively). The SDS- PAGE profiles of the extracted protein showed change in gene expression for protein profiles comparing with control. The incubation these bacteria in effluent increase superoxide dismutase (SOD) isozyme activity.
{"title":"EFFECT OF EFFLUENT ON BACTERIA COMPARING WITH TAP WATER","authors":"H. Ahmed, Ragaa A. Homouda","doi":"10.21608/EJGC.2014.9933","DOIUrl":"https://doi.org/10.21608/EJGC.2014.9933","url":null,"abstract":"Water is important for all the living things but nowadays, it has been polluted with inorganic contaminants which are discharged from industries. This study was carried out to evaluate the potential of Rhizobium leguminosarum, Azotobacter chrocoocum, Serratia marcescns and Bacillus circulans to survive in effluent obtained from Alexandria and Sadat City and the ability of these bacteria to remove metals from effluent. Samples of effluent were assessed for various physicochemical parameters like pH, Electrical conductivity (EC), concentration of salts, osmotic pressure, biological oxygen demand (BOD) (35 and 65), chemical oxygen demand (COD) (94 and 135) in Alexandria and Sadat City, respectively. The pH was 7.58 and 7.78, respectively compared with tap water (control) pH from Sadat City and Alexandria 7.4-7.2, respectively. It was found that different the concentrations of media containing effluent obtained from Alexandria and Sadat City having negative effect on bacterial growth and the more effective of Sadat City effluent, except Bacillus circulans had the same trend. The examined bacteria were capable of removing metals from different sources of effluent (Alexandria and Sadat City), Cu++ ions was highest removed by Rhizobium leguminosarum (44% and 39%), Azotobacter chrocoocum (42% and 50%), Serratia marcescns (50% and 50%) and Bacillus circulans (36% and 46%, respectively). The SDS- PAGE profiles of the extracted protein showed change in gene expression for protein profiles comparing with control. The incubation these bacteria in effluent increase superoxide dismutase (SOD) isozyme activity.","PeriodicalId":31811,"journal":{"name":"Egyptian Journal of Genetics and Cytology","volume":"43 1","pages":"59-71"},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68479662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Water stress effects on three barley (Hordeum vulgare L.) genotypes in field and lab were studied. The genotypic responses varied among growth stages according to water stress. Lab experiments showed that germination percentage, shoot length, root length and total dry mater were the most effective traits between sensitive and tolerant genotypes. Giza 126 and California Marriott were tolerant and stable under different stress levels, while the sensitive variety Giza 129 showed sharp decrease in germination percentage, shoot length and total dry mater. Giza 126 recorded the highest yield and yield component values under stress condition as well as in well-irrigated condition, followed by California Marriott under the stress condition. Giza 129 ranked last under stress condition. Our results showed that the HVB23D SSR primer and the specific primer LEA generated specific bands occurred in the studied drought tolerant barley genotypes (Giza 126 and California Marriott), while they were absent in the sensitive genotype Giza 129.
{"title":"MOLECULAR AND FIELD ANALYSIS OF SOME BARLEY GENO-TYPES FOR WATER STRESS TOLERANCE","authors":"M. E. El-denary, E. E. EL-Shawy","doi":"10.21608/EJGC.2014.9940","DOIUrl":"https://doi.org/10.21608/EJGC.2014.9940","url":null,"abstract":"Water stress effects on three barley (Hordeum vulgare L.) genotypes in field and lab were studied. The genotypic responses varied among growth stages according to water stress. Lab experiments showed that germination percentage, shoot length, root length and total dry mater were the most effective traits between sensitive and tolerant genotypes. Giza 126 and California Marriott were tolerant and stable under different stress levels, while the sensitive variety Giza 129 showed sharp decrease in germination percentage, shoot length and total dry mater. Giza 126 recorded the highest yield and yield component values under stress condition as well as in well-irrigated condition, followed by California Marriott under the stress condition. Giza 129 ranked last under stress condition. Our results showed that the HVB23D SSR primer and the specific primer LEA generated specific bands occurred in the studied drought tolerant barley genotypes (Giza 126 and California Marriott), while they were absent in the sensitive genotype Giza 129.","PeriodicalId":31811,"journal":{"name":"Egyptian Journal of Genetics and Cytology","volume":"43 1","pages":"187-198"},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68480337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The present investigations were aimed to develop a high efficiency of in vitro regeneration and genetic transformation systems of Moringa oleifera Lam from nodal segments of young aseptically grown seedlings using Agrobacterium-mediated transformation approach. Frequency of responded explants and number of shoots per explant were recorded during the course of the regeneration experiment. Regeneration capacity of nodal segments was evaluated on Murashige and Skoog (MS) media supplemented with 18 different combinations of plant growth regulators of benzylaminopurine (BAP), Zeatine (Zea) and naphthaleneacetic acid (NAA). Application of 1.0 mg/l BAP individually was found to be superior in terms of highest number of responded explants (95.7%) as well as the highest average (6.6) of axillary shoot developments per explant with direct emerging of adventitious shoots escaping callus formations. Well developed shoots subjected to rooting media supplemented with IAA or IBA or their different combinations. The most successful rooting events (100%) for regenerated shoots were obtained on rooting media containing ½ MS salts and supplemented with 1.0 mg/l IBA along with 0.5 mg/l IAA within three weeks maximum. The plant transformation vector pBIN121 harbors both the uidA (GUS) and NPTII (kanamycin resistant) genes were used to establish the Agrobacterium-mediated transformation experiment. Number of Putative transformed young shoots that developed onto Kanamycin selective regeneration medium were recorded representing 39.6% transformation efficiency. PCR analysis was carried out to verify successful transformation and gene integration for both GUS and NPTll genes in randomly selected young shoots while Histochemical GUS assay confirmed the successful expression of GUS gene in different parts of the putative transgenic plantlets.
{"title":"DEVELOPMENT OF in vitro REGENERATION AND Agrobacterium MEDIATED TRANSFORMATION SYSTEMS FOR Moringa oleifera","authors":"D. Abbas","doi":"10.21608/EJGC.2014.9935","DOIUrl":"https://doi.org/10.21608/EJGC.2014.9935","url":null,"abstract":"The present investigations were aimed to develop a high efficiency of in vitro regeneration and genetic transformation systems of Moringa oleifera Lam from nodal segments of young aseptically grown seedlings using Agrobacterium-mediated transformation approach. Frequency of responded explants and number of shoots per explant were recorded during the course of the regeneration experiment. Regeneration capacity of nodal segments was evaluated on Murashige and Skoog (MS) media supplemented with 18 different combinations of plant growth regulators of benzylaminopurine (BAP), Zeatine (Zea) and naphthaleneacetic acid (NAA). Application of 1.0 mg/l BAP individually was found to be superior in terms of highest number of responded explants (95.7%) as well as the highest average (6.6) of axillary shoot developments per explant with direct emerging of adventitious shoots escaping callus formations. Well developed shoots subjected to rooting media supplemented with IAA or IBA or their different combinations. The most successful rooting events (100%) for regenerated shoots were obtained on rooting media containing ½ MS salts and supplemented with 1.0 mg/l IBA along with 0.5 mg/l IAA within three weeks maximum. The plant transformation vector pBIN121 harbors both the uidA (GUS) and NPTII (kanamycin resistant) genes were used to establish the Agrobacterium-mediated transformation experiment. Number of Putative transformed young shoots that developed onto Kanamycin selective regeneration medium were recorded representing 39.6% transformation efficiency. PCR analysis was carried out to verify successful transformation and gene integration for both GUS and NPTll genes in randomly selected young shoots while Histochemical GUS assay confirmed the successful expression of GUS gene in different parts of the putative transgenic plantlets.","PeriodicalId":31811,"journal":{"name":"Egyptian Journal of Genetics and Cytology","volume":"43 1","pages":"99-111"},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68479804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Our previous studies showed that allelopathic activity and momilactone B concentration in rice seedlings were increased (6.9 times) in the presence of barnyardgrass seedlings. In this study we attempt to demonstrate the response of rice to the grass and Upregulation of the putative genes that encode phenyl-alanine ammonialyase (PAL), O-methyltransferase, triosephosphate-isomerase, and cytochrome P450, which are involved in phenolic allelochemicals synthesis. Suppression subtractive hybridization (SSH) technique was used in this study to investigate the up-regulate expression of genes in Rikuto Norin22 exposed to low N levels and cocultured with/without barnyardgrass in hydroponics. PAL is the first key enzyme in phenylpropanoid metabolism that can be regulated by various biotic and abiotic factors to different extents depending on different plant species and P450 is directly involved in the formation of pcoumaric acid. Rice genotypes, Giza182 like accession Rikuto Norin22 has strong allelopathic activity. In addition, our studies indicate that the accessions Giza182 is restorer line (data is not shown). So, we recommend using accession Giza182 as parental line in producing hybrid rice that has high yield and strong allelopathy like the hybrid (IR69625A x Giza182).
{"title":"ANALYSIS OF GENE EXPRESSIONS ASSOCIATED WITH INCREASED ALLELOPATHY IN RICE (Oryza sativa L.) UNDER LOW NITROGEN TREATMENT","authors":"M. E. El-denary, E. ElShamey","doi":"10.21608/EJGC.2014.9936","DOIUrl":"https://doi.org/10.21608/EJGC.2014.9936","url":null,"abstract":"Our previous studies showed that allelopathic activity and momilactone B concentration in rice seedlings were increased (6.9 times) in the presence of barnyardgrass seedlings. In this study we attempt to demonstrate the response of rice to the grass and Upregulation of the putative genes that encode phenyl-alanine ammonialyase (PAL), O-methyltransferase, triosephosphate-isomerase, and cytochrome P450, which are involved in phenolic allelochemicals synthesis. Suppression subtractive hybridization (SSH) technique was used in this study to investigate the up-regulate expression of genes in Rikuto Norin22 exposed to low N levels and cocultured with/without barnyardgrass in hydroponics. PAL is the first key enzyme in phenylpropanoid metabolism that can be regulated by various biotic and abiotic factors to different extents depending on different plant species and P450 is directly involved in the formation of pcoumaric acid. Rice genotypes, Giza182 like accession Rikuto Norin22 has strong allelopathic activity. In addition, our studies indicate that the accessions Giza182 is restorer line (data is not shown). So, we recommend using accession Giza182 as parental line in producing hybrid rice that has high yield and strong allelopathy like the hybrid (IR69625A x Giza182).","PeriodicalId":31811,"journal":{"name":"Egyptian Journal of Genetics and Cytology","volume":"43 1","pages":"113-131"},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68479918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The antioxidant activity status is important to determine the nutritional value of fruits and vegetables. Increased antioxidant levels not only have high benefits in preventing widespread human diseases, including cancer and cardiovascular pathogens but also it enhances shelf life as well. This study was carried out to test the effects of 100 mM NaCl on some antioxidants (vitamins C and E) and some monosaccharides (glucose and fructose) of TYLCV virus-free tomato plants (Solanum lycopersicon) cultivar Castle Rock. The plants were treated at 30 days old with 100 mM NaCl for the next 45 days. At 75 days old, mature leaves were harvested and tested for TYLCV infection, as this virus affects the amount of antioxidants in tomato plants. Plants were kept in cages to avoid infection with TYLCV. The plants gave negative results, confirming that they are virus-free. Levels of vitamin C and vitamin E were determined by a reverse-phase HPLC technique while levels of glucose and fructose were determined using UPLC-MS in 75 days treated tomato leaves. Plants showed an increase in vitamin C, α-tochopherol, glucose and a slight increase in fructose contents versus the control plants. These results are of great importance from the nutritional and health points of view where salt stress improved the plant quality by increasing the concentrations of important antioxidants (vitamin C and vitamin E). In conclusion, the use of controlled salinity level can be an effective method to produce tomatoes of good nutritional quality and with higher market price.
水果和蔬菜的抗氧化活性状况是决定其营养价值的重要因素。增加抗氧化水平不仅对预防广泛传播的人类疾病有很高的好处，包括癌症和心血管病原体，而且还能延长保质期。本试验研究了100 mM NaCl处理对脱毒番茄“城堡岩”抗氧化剂(维生素C和维生素E)和单糖(葡萄糖和果糖)的影响。在植株生长30 d时，用100 mM NaCl处理45 d。在75天大的时候，收获成熟的叶片并测试TYLCV感染，因为这种病毒会影响番茄植株中抗氧化剂的数量。将植物置于笼中以避免感染TYLCV。这些植物给出了阴性结果，证实它们是无病毒的。采用反相高效液相色谱法测定处理75天的番茄叶片中维生素C和维生素E的含量，UPLC-MS测定处理75天的番茄叶片中葡萄糖和果糖的含量。与对照植物相比，植物的维生素C、α-生育酚、葡萄糖含量增加，果糖含量略有增加。从营养和健康的角度来看，盐胁迫通过增加重要抗氧化剂(维生素C和维生素E)的浓度来改善植株品质。综上所述，控制盐度是生产营养品质高、市场价格高的番茄的有效方法。
{"title":"IMPROVEMENT OF SOME NUTRITIONAL VALUES OF TOMATOES VIA SALINITY","authors":"Nahla El-Sherif, L. El-Gaied","doi":"10.21608/EJGC.2014.9937","DOIUrl":"https://doi.org/10.21608/EJGC.2014.9937","url":null,"abstract":"The antioxidant activity status is important to determine the nutritional value of fruits and vegetables. Increased antioxidant levels not only have high benefits in preventing widespread human diseases, including cancer and cardiovascular pathogens but also it enhances shelf life as well. This study was carried out to test the effects of 100 mM NaCl on some antioxidants (vitamins C and E) and some monosaccharides (glucose and fructose) of TYLCV virus-free tomato plants (Solanum lycopersicon) cultivar Castle Rock. The plants were treated at 30 days old with 100 mM NaCl for the next 45 days. At 75 days old, mature leaves were harvested and tested for TYLCV infection, as this virus affects the amount of antioxidants in tomato plants. Plants were kept in cages to avoid infection with TYLCV. The plants gave negative results, confirming that they are virus-free. Levels of vitamin C and vitamin E were determined by a reverse-phase HPLC technique while levels of glucose and fructose were determined using UPLC-MS in 75 days treated tomato leaves. Plants showed an increase in vitamin C, α-tochopherol, glucose and a slight increase in fructose contents versus the control plants. These results are of great importance from the nutritional and health points of view where salt stress improved the plant quality by increasing the concentrations of important antioxidants (vitamin C and vitamin E). In conclusion, the use of controlled salinity level can be an effective method to produce tomatoes of good nutritional quality and with higher market price.","PeriodicalId":31811,"journal":{"name":"Egyptian Journal of Genetics and Cytology","volume":"43 1","pages":"133-142"},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68479970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Mohammed, E. Metry, I. Ismail, H. El-Shishtawy, G. G. El-Karim, T. N. El-Din
The potato tuber moth, Phthorimaea operculella (Zeller), is the major destructive insect of potato. The larvae attack foliage and tubers in the field and in storage as well. Over the past years, there was a tremendous effort to produce transgenic potato cultivar that is resistant to the larval infestation of potato tuber moth. ‘Spunta’ potato cultivar was previously transformed with Bt-cry5 gene under 35S promoter construct. Three transgenic ‘Spunta’ lines, ‘Spunta G2’, ‘Spunta G3’ and ‘Spunta 6a-3’, were selected for their high resistance to PTM attack. After 14 years of storage, these three lines are subjected to in vitro micropropagation, tuberization and reevaluation. Both PCR and RT-PCR confirmed the presence of cry5 gene within ‘Spunta’ genome and its expression within potato plant tissues. Amplified fragment length polymorphism (AFLP) analysis revealed that the percentage of polymorphism among the three lines and non-transgenic ranged from 0% to 10.6%. Two lines ‘Spunta G3’ and ‘Spunta 6a-3’ were selected for field agronomical performances as steps for environmental safety as well as PTM challenge. Both lines are highly resistant to PTM compared to the control, only 3% of the yield shows susceptibility to larval penetration of the tubers.
{"title":"EVALUATION OF TRANSGENIC POTATO LINES RESISTANCE TO POTATO TUBER MOTH, Phthorimaea operculella (ZELLER)","authors":"A. Mohammed, E. Metry, I. Ismail, H. El-Shishtawy, G. G. El-Karim, T. N. El-Din","doi":"10.21608/EJGC.2014.9939","DOIUrl":"https://doi.org/10.21608/EJGC.2014.9939","url":null,"abstract":"The potato tuber moth, Phthorimaea operculella (Zeller), is the major destructive insect of potato. The larvae attack foliage and tubers in the field and in storage as well. Over the past years, there was a tremendous effort to produce transgenic potato cultivar that is resistant to the larval infestation of potato tuber moth. ‘Spunta’ potato cultivar was previously transformed with Bt-cry5 gene under 35S promoter construct. Three transgenic ‘Spunta’ lines, ‘Spunta G2’, ‘Spunta G3’ and ‘Spunta 6a-3’, were selected for their high resistance to PTM attack. After 14 years of storage, these three lines are subjected to in vitro micropropagation, tuberization and reevaluation. Both PCR and RT-PCR confirmed the presence of cry5 gene within ‘Spunta’ genome and its expression within potato plant tissues. Amplified fragment length polymorphism (AFLP) analysis revealed that the percentage of polymorphism among the three lines and non-transgenic ranged from 0% to 10.6%. Two lines ‘Spunta G3’ and ‘Spunta 6a-3’ were selected for field agronomical performances as steps for environmental safety as well as PTM challenge. Both lines are highly resistant to PTM compared to the control, only 3% of the yield shows susceptibility to larval penetration of the tubers.","PeriodicalId":31811,"journal":{"name":"Egyptian Journal of Genetics and Cytology","volume":"43 1","pages":"173-186"},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68480244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
E. A. Eissa, G. Farahat, B. Mahmoud, E. A. El-Full
The aims of the present study were to characterize the possible genetic and productive traits differences associated with the plumage colour in two genotypes of Japanese quail. Productive performance and DNA markers were used to identify these genotypes. Genotype effect showed that the brown genotype had significantly heavier body weight (BW) at 7, 14 and 21 days of age and longer shank length (SL) at all studied ages, except for 1 and 14 days than the white genotype. Sex effect on BW and SL was significant at all studied ages, except for one day old, females had significantly higher BW and SL than males. The interaction effect between genotype and sex on BW was significant at all studied ages except for one day old. The interaction effect between genotype and sex on SL was significant at all studied ages, except for 1 and 14 days. Brown genotype had significantly heavier BW at first egg than the white genotype. The white genotype had significantly higher shape index than brown genotype. The brown genotype matured at earlier age (P≤0.05) than the white genotype and had shorter days (P≤0.05) to produce the first 30 eggs, and had shorter days (P>0.05) to produce the first 10 eggs than the brown genotype. Brown genotype laid significantly more number of eggs during the first, the second and the first two months than the white genotype and had significant higher egg mass during the different study periods. From the present results, it can be concluded that brown genotype had favored growth traits and most of egg production related traits during annual egg production. The level of polymorphism among two Japanese quail genotypes brown and white, was estimated using two PCR-based marker techniques RAPD and ISSRs. Six RAPD and six ISSRs primers were employed to find out genetic variations and relationships among these genotypes of quail. RAPD and ISSRs analysis generated a total number of 442 and 467 amplicons representing a level of polymorphism of 74.24% and 72.86%, and an average number of polymorphic fragments/ primer of 8.17 and 8.5, respectively. The genetic relationships among the 10 individuals of quail were estimated in terms of similarity using Dice coefficients. The genetic similarity ranged from 0.00-1.00 for RAPD, ISSRs, and RAPD and ISSRs combination. The intergenotype relationships among the two quail genotypes based on RAPD, ISSRs, and RAPD and ISSRs combination revealed the highest genetic similarity between the genotype brown male (5) and brown female (2), white female (7) and brown female (1), and white female (6) and brown female (1), respectively. The intergenotype relationships among the two quail genotypes based on RAPD, ISSRs, and RAPD and ISSRs combination revealed the lowest genetic similarity between the genotype brown female (2) and brown female (1), white female (7) and white female (6), and white female (7) and white female (6), respectively. The RAPD based dendrogram clustered the brown genotype female and male, and white g
{"title":"PRODUCTIVE PERFORMANCE AND MOLECULAR GENETIC CHARACTERIZATION OF BROWN AND WHITE JAPANESE QUAIL GENOTYPES USING RAPD AND ISSRs-PCR MARKERS","authors":"E. A. Eissa, G. Farahat, B. Mahmoud, E. A. El-Full","doi":"10.21608/EJGC.2014.9934","DOIUrl":"https://doi.org/10.21608/EJGC.2014.9934","url":null,"abstract":"The aims of the present study were to characterize the possible genetic and productive traits differences associated with the plumage colour in two genotypes of Japanese quail. Productive performance and DNA markers were used to identify these genotypes. Genotype effect showed that the brown genotype had significantly heavier body weight (BW) at 7, 14 and 21 days of age and longer shank length (SL) at all studied ages, except for 1 and 14 days than the white genotype. Sex effect on BW and SL was significant at all studied ages, except for one day old, females had significantly higher BW and SL than males. The interaction effect between genotype and sex on BW was significant at all studied ages except for one day old. The interaction effect between genotype and sex on SL was significant at all studied ages, except for 1 and 14 days. Brown genotype had significantly heavier BW at first egg than the white genotype. The white genotype had significantly higher shape index than brown genotype. The brown genotype matured at earlier age (P≤0.05) than the white genotype and had shorter days (P≤0.05) to produce the first 30 eggs, and had shorter days (P>0.05) to produce the first 10 eggs than the brown genotype. Brown genotype laid significantly more number of eggs during the first, the second and the first two months than the white genotype and had significant higher egg mass during the different study periods. From the present results, it can be concluded that brown genotype had favored growth traits and most of egg production related traits during annual egg production. The level of polymorphism among two Japanese quail genotypes brown and white, was estimated using two PCR-based marker techniques RAPD and ISSRs. Six RAPD and six ISSRs primers were employed to find out genetic variations and relationships among these genotypes of quail. RAPD and ISSRs analysis generated a total number of 442 and 467 amplicons representing a level of polymorphism of 74.24% and 72.86%, and an average number of polymorphic fragments/ primer of 8.17 and 8.5, respectively. The genetic relationships among the 10 individuals of quail were estimated in terms of similarity using Dice coefficients. The genetic similarity ranged from 0.00-1.00 for RAPD, ISSRs, and RAPD and ISSRs combination. The intergenotype relationships among the two quail genotypes based on RAPD, ISSRs, and RAPD and ISSRs combination revealed the highest genetic similarity between the genotype brown male (5) and brown female (2), white female (7) and brown female (1), and white female (6) and brown female (1), respectively. The intergenotype relationships among the two quail genotypes based on RAPD, ISSRs, and RAPD and ISSRs combination revealed the lowest genetic similarity between the genotype brown female (2) and brown female (1), white female (7) and white female (6), and white female (7) and white female (6), respectively. The RAPD based dendrogram clustered the brown genotype female and male, and white g","PeriodicalId":31811,"journal":{"name":"Egyptian Journal of Genetics and Cytology","volume":"43 1","pages":"73-98"},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68479720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The ISSR markers have been used in order to determine genetic variation and relationship between two clover ecotypes. Six primers for ISSR-PCR technique and succeeded reliable amplicons for different types of Egyptian clover ecotypes. The results revealed polymorphisms level re-vealed by ISSR primers. HB-10 ISSR-primer was better than of the rest ISSR primers in polymorphic 100%. The Fahl monocut ecotype had 29 present bands, 2 absent bands in 31 total bands; also Fahl have two unique bands. The multicut ecotype Gemmiza 1, Giza 6 and Serw1 were given 20 present, 11 absent; 21 present and 10 absent and 23 present and 11 absent respectively. The three unique bands were appeared in two ecotypes. Fahl was given two with HB-11 and HB-13; the Serw multicut cultivar had one unique bands with HB-08. Similarity indices among the four Egyptian clover cultivars based on ISSR analysis was estimated the highest value was appeared between Fahl and Gemmiza 1 as well as Giza 6 and Serw 1 followed by Fahl and Serw 1. The lowest similarity value was appeared between Gemmiza 1 and Serw 1 followed by Gemmiza 1 and Giza 6.
{"title":"GENETIC VARIATION BETWEEN TWO ECOTYPES OF EGYPTIAN CLOVER BY ISSR TECHNIQUE","authors":"E. Zayed, M. Sayed","doi":"10.21608/EJGC.2013.9965","DOIUrl":"https://doi.org/10.21608/EJGC.2013.9965","url":null,"abstract":"The ISSR markers have been used in order to determine genetic variation and relationship between two clover ecotypes. Six primers for ISSR-PCR technique and succeeded reliable amplicons for different types of Egyptian clover ecotypes. The results revealed polymorphisms level re-vealed by ISSR primers. HB-10 ISSR-primer was better than of the rest ISSR primers in polymorphic 100%. The Fahl monocut ecotype had 29 present bands, 2 absent bands in 31 total bands; also Fahl have two unique bands. The multicut ecotype Gemmiza 1, Giza 6 and Serw1 were given 20 present, 11 absent; 21 present and 10 absent and 23 present and 11 absent respectively. The three unique bands were appeared in two ecotypes. Fahl was given two with HB-11 and HB-13; the Serw multicut cultivar had one unique bands with HB-08. Similarity indices among the four Egyptian clover cultivars based on ISSR analysis was estimated the highest value was appeared between Fahl and Gemmiza 1 as well as Giza 6 and Serw 1 followed by Fahl and Serw 1. The lowest similarity value was appeared between Gemmiza 1 and Serw 1 followed by Gemmiza 1 and Giza 6.","PeriodicalId":31811,"journal":{"name":"Egyptian Journal of Genetics and Cytology","volume":"42 1","pages":"247-258"},"PeriodicalIF":0.0,"publicationDate":"2013-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68478242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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