Background and aims: The abnormal increase in blood cholesterol can cause many problems. Statins have a cholesterol-lowering effect, but they also have adverse effects. Garlic prevents the formation of cholesterol due to its antibiotic properties. This study aimed to investigate the comparative effect of garlic powder and atorvastatin on hypercholesterolemia-induced reproductive failure in female rats. Methods: In the present experimental study, 48 adult female Wistar rats were divided into eight groups (n=6), including control, atorvastatin (10 mg/kg/d; orally), atorvastatin (20 mg/kg/d; orally), garlic powder (100 mg/kg/d; orally), hypercholesterolemia (1.5 mg/kg/d of cholesterol; orally), hypercholesterolemia + atorvastatin (10 mg/kg/d), hypercholesterolemia + atorvastatin (20 mg/kg/d), and hypercholesterolemia + garlic powder. After 30 days, rats were euthanized and blood samples were obtained from their heart for serological assessments. The right ovary was transferred to 10% formalin for histological analyses, and the left ovary was transferred to a −80°C freezer for evaluation of oxidative stress markers. Data were statistically analyzed by ANOVA and Tukey’s test using SPSS version 24.0 (P<0.05). Results: The number of healthy primordial, primary, secondary, and antral follicles, catalase activity, total antioxidant capacity (TAOC) as well as estrogen and progesterone levels were lower in hypercholesterolemic rats compared to controls (P<0.001). Additionally, the number of the atretic primary, secondary, and antral follicles and malondialdehyde (MDA) levels were higher in hypercholesterolemic rats (P<0.001). However, garlic powder and atorvastatin 10 improved alterations in the mentioned parameters (P=0.99). Conclusion: The results showed that hypercholesterolemia could have adverse effects on rat ovaries. However, the garlic powder improves ovarian toxicity in hypercholesterolemia rats better than atorvastatin.
{"title":"Comparative effects of garlic (Allium sativum) powder and atorvastatin in female reproductive system of hypercholesterolemic rats: A histological and biochemical evaluation","authors":"Sima Jafari, F. Farokhi, A. Sadeghi","doi":"10.34172/jsums.2021.22","DOIUrl":"https://doi.org/10.34172/jsums.2021.22","url":null,"abstract":"Background and aims: The abnormal increase in blood cholesterol can cause many problems. Statins have a cholesterol-lowering effect, but they also have adverse effects. Garlic prevents the formation of cholesterol due to its antibiotic properties. This study aimed to investigate the comparative effect of garlic powder and atorvastatin on hypercholesterolemia-induced reproductive failure in female rats. Methods: In the present experimental study, 48 adult female Wistar rats were divided into eight groups (n=6), including control, atorvastatin (10 mg/kg/d; orally), atorvastatin (20 mg/kg/d; orally), garlic powder (100 mg/kg/d; orally), hypercholesterolemia (1.5 mg/kg/d of cholesterol; orally), hypercholesterolemia + atorvastatin (10 mg/kg/d), hypercholesterolemia + atorvastatin (20 mg/kg/d), and hypercholesterolemia + garlic powder. After 30 days, rats were euthanized and blood samples were obtained from their heart for serological assessments. The right ovary was transferred to 10% formalin for histological analyses, and the left ovary was transferred to a −80°C freezer for evaluation of oxidative stress markers. Data were statistically analyzed by ANOVA and Tukey’s test using SPSS version 24.0 (P<0.05). Results: The number of healthy primordial, primary, secondary, and antral follicles, catalase activity, total antioxidant capacity (TAOC) as well as estrogen and progesterone levels were lower in hypercholesterolemic rats compared to controls (P<0.001). Additionally, the number of the atretic primary, secondary, and antral follicles and malondialdehyde (MDA) levels were higher in hypercholesterolemic rats (P<0.001). However, garlic powder and atorvastatin 10 improved alterations in the mentioned parameters (P=0.99). Conclusion: The results showed that hypercholesterolemia could have adverse effects on rat ovaries. However, the garlic powder improves ovarian toxicity in hypercholesterolemia rats better than atorvastatin.","PeriodicalId":318974,"journal":{"name":"Journal of Shahrekord University of Medical Sciences","volume":"31 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122273727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background and aims: The aim of the present study was to investigate the main causes of traumatic spinal cord injury (TSCI), along with the relationship between SCI patients’ demographic characteristics and related treatments. Methods: In general, 608 patients suffering from TSCI and referring to Ayatollah Kashani hospital, Shahrekord in 2016-2017 were enrolled in this cross-sectional study. Patients’ demographic characteristics, level of injury, the severity of the injury, injury cause, and duration of hospitalization (DOH) were obtained according to their files and medical records. Several months after discharge, the patients were examined by a neurosurgeon, and the treatment outcome was recorded in specific checklists. Results: The mean age of the injured individuals was 34.2±16.9 years and the majority of the injured (70.2%) were males. ASIA grades E and D were also reported in 50.3% and 25% of the injured at admission, respectively. Further, grade E injury was observed in 77.1% of the injured at discharge. Medicinal, non-surgical, and surgical treatments were used for 53.8%, 25.8%, and 20.4% of patients, respectively. The levels of injury, treatment strategy, and clinical outcomes were significantly different based on the ASIA grades at admission (P<0.001, for all items). There was a significant difference among DOH (day) in terms of the level of injury, ASIA grades during admission, treatment strategy, and treatment outcomes in different individuals (P<0.001, for all items). Conclusion: Regarding the relationship between the treatment strategy, the ASIA grade, and the outcome of the treatment, it seems necessary to perform surgical or rehabilitative interventions for each person in accordance with demographic characteristics.
{"title":"Relationship between demographic characteristics, spinal impairment, and interventional strategies in the clinical outcome of spinal cord injury patients","authors":"M. Dayani, Sepideh Safdarian, A. Rostamzadeh","doi":"10.34172/jsums.2021.18","DOIUrl":"https://doi.org/10.34172/jsums.2021.18","url":null,"abstract":"Background and aims: The aim of the present study was to investigate the main causes of traumatic spinal cord injury (TSCI), along with the relationship between SCI patients’ demographic characteristics and related treatments. Methods: In general, 608 patients suffering from TSCI and referring to Ayatollah Kashani hospital, Shahrekord in 2016-2017 were enrolled in this cross-sectional study. Patients’ demographic characteristics, level of injury, the severity of the injury, injury cause, and duration of hospitalization (DOH) were obtained according to their files and medical records. Several months after discharge, the patients were examined by a neurosurgeon, and the treatment outcome was recorded in specific checklists. Results: The mean age of the injured individuals was 34.2±16.9 years and the majority of the injured (70.2%) were males. ASIA grades E and D were also reported in 50.3% and 25% of the injured at admission, respectively. Further, grade E injury was observed in 77.1% of the injured at discharge. Medicinal, non-surgical, and surgical treatments were used for 53.8%, 25.8%, and 20.4% of patients, respectively. The levels of injury, treatment strategy, and clinical outcomes were significantly different based on the ASIA grades at admission (P<0.001, for all items). There was a significant difference among DOH (day) in terms of the level of injury, ASIA grades during admission, treatment strategy, and treatment outcomes in different individuals (P<0.001, for all items). Conclusion: Regarding the relationship between the treatment strategy, the ASIA grade, and the outcome of the treatment, it seems necessary to perform surgical or rehabilitative interventions for each person in accordance with demographic characteristics.","PeriodicalId":318974,"journal":{"name":"Journal of Shahrekord University of Medical Sciences","volume":"37 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114491017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Zahra Keivani Hafshejani, Z. Lorigooini, F. Deris, Neda Akbari, F. Farahbod, S. Fazelian, F. Moradi, M. Dehghan
Background and aims: Osteoporosis is one of the most common metabolic bone diseases with systemic involvement of the body skeleton. The Peganum harmala seed contains high amounts of carboline alkaloids, which have been shown to have positive effects on bone formation in animal studies. In the present study, the effect of an oral capsule of P. harmala seed on bone density was evaluated in menopausal women prone to osteoporosis. Methods: In this randomized controlled clinical trial, 100 women referring to the orthopedic clinic with a diagnosis of osteoporosis were included and divided into the intervention group treated with calcium D (500 mg) twice a day, Osteofos (70 mg) per week, and P. harmala (500 mg) twice‐a‐day, and the control group treated with calcium D and Osteofos. Before and three months after the intervention, patients were evaluated for osteoporosis using bone densitometry. Finally, independent t-test, paired t-test, and repeated measures ANOVA were used for statistical analysis. Results: The mean bone mineral density (BMD) of the femur before and after the intervention showed significant improvements in the intervention and control groups (P<0.001). The mean differences in BMD before and after the intervention were significant in both control and intervention groups with higher improvements in the intervention group (P<0.001). Although the mean BMD of the spine before the intervention was not significantly different between the two groups (P=0.167), it was better in the intervention group after the intervention (P=0.030). Conclusion: The findings of the present study confirmed the beneficial effects of P. harmala on osteoporosis while the lack of any changes in liver enzymes.
{"title":"Effect of oral capsule of Peganum harmala seeds on bone density in menopausal women prone to osteoporosis","authors":"Zahra Keivani Hafshejani, Z. Lorigooini, F. Deris, Neda Akbari, F. Farahbod, S. Fazelian, F. Moradi, M. Dehghan","doi":"10.34172/jsums.2021.23","DOIUrl":"https://doi.org/10.34172/jsums.2021.23","url":null,"abstract":"Background and aims: Osteoporosis is one of the most common metabolic bone diseases with systemic involvement of the body skeleton. The Peganum harmala seed contains high amounts of carboline alkaloids, which have been shown to have positive effects on bone formation in animal studies. In the present study, the effect of an oral capsule of P. harmala seed on bone density was evaluated in menopausal women prone to osteoporosis. Methods: In this randomized controlled clinical trial, 100 women referring to the orthopedic clinic with a diagnosis of osteoporosis were included and divided into the intervention group treated with calcium D (500 mg) twice a day, Osteofos (70 mg) per week, and P. harmala (500 mg) twice‐a‐day, and the control group treated with calcium D and Osteofos. Before and three months after the intervention, patients were evaluated for osteoporosis using bone densitometry. Finally, independent t-test, paired t-test, and repeated measures ANOVA were used for statistical analysis. Results: The mean bone mineral density (BMD) of the femur before and after the intervention showed significant improvements in the intervention and control groups (P<0.001). The mean differences in BMD before and after the intervention were significant in both control and intervention groups with higher improvements in the intervention group (P<0.001). Although the mean BMD of the spine before the intervention was not significantly different between the two groups (P=0.167), it was better in the intervention group after the intervention (P=0.030). Conclusion: The findings of the present study confirmed the beneficial effects of P. harmala on osteoporosis while the lack of any changes in liver enzymes.","PeriodicalId":318974,"journal":{"name":"Journal of Shahrekord University of Medical Sciences","volume":"352 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122843198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Shabanian, A. Khoshdel, Majid Dezfouli, F. Famouri
Background and aims: Preterm birth can cause high morbidity and mortality in women. Previous evidence has confirmed the association between zinc (Zn) deficiency in x women and some pregnancy complications. This study investigated the association between serum Zn concentration in pregnant women and preterm birth. Methods: This case-control study focused on evaluating 76 pregnant women with preterm birth (case group) and 62 pregnant women with term birth (control group) and was conducted in the obstetrics ward of Hajar hospital, Shahrekord, Iran in 2014. The Zn level was measured by spectrophotometry and data were analyzed by SPSS, version 15. Results: The prevalence of Zn deficiency was 95.6%. The mean of serum Zn concentration was 39.62±11.83 and 59.81±8.8 in the preterm and term delivery groups, respectively (P<0.001). Similarly, the mean of serum Zn concentrations was 43.06±15.6 and 50.46±13.8 in women with and without the rupture of pregnancy membranes, respectively (P=0.01). Based on the findings, the serum Zn concentration was not significantly associated with parity (P=0.634). Conclusion: Although a decrease in the serum Zn concentration could lead to premature rupture of membranes during pregnancy and preterm birth, it could not be considered as the main factor for preterm birth. In addition, Zn deficiency was highly prevalent in pregnant women. Therefore, nutritional interventions should be performed to prevent complications due to the deficiency of micronutrients such as Zn so that to increase health maintenance in mothers and children.
{"title":"Study of the association between serum zinc concentration in pregnant women and preterm birth","authors":"S. Shabanian, A. Khoshdel, Majid Dezfouli, F. Famouri","doi":"10.34172/jsums.2021.17","DOIUrl":"https://doi.org/10.34172/jsums.2021.17","url":null,"abstract":"Background and aims: Preterm birth can cause high morbidity and mortality in women. Previous evidence has confirmed the association between zinc (Zn) deficiency in x women and some pregnancy complications. This study investigated the association between serum Zn concentration in pregnant women and preterm birth. Methods: This case-control study focused on evaluating 76 pregnant women with preterm birth (case group) and 62 pregnant women with term birth (control group) and was conducted in the obstetrics ward of Hajar hospital, Shahrekord, Iran in 2014. The Zn level was measured by spectrophotometry and data were analyzed by SPSS, version 15. Results: The prevalence of Zn deficiency was 95.6%. The mean of serum Zn concentration was 39.62±11.83 and 59.81±8.8 in the preterm and term delivery groups, respectively (P<0.001). Similarly, the mean of serum Zn concentrations was 43.06±15.6 and 50.46±13.8 in women with and without the rupture of pregnancy membranes, respectively (P=0.01). Based on the findings, the serum Zn concentration was not significantly associated with parity (P=0.634). Conclusion: Although a decrease in the serum Zn concentration could lead to premature rupture of membranes during pregnancy and preterm birth, it could not be considered as the main factor for preterm birth. In addition, Zn deficiency was highly prevalent in pregnant women. Therefore, nutritional interventions should be performed to prevent complications due to the deficiency of micronutrients such as Zn so that to increase health maintenance in mothers and children.","PeriodicalId":318974,"journal":{"name":"Journal of Shahrekord University of Medical Sciences","volume":"288 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122795614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N. Xodadadi, A. Saeidinia, M. Zeinoddini, R. Khalilzadeh
Background and aims: Human interferon beta-1a (hIFNβ-1a) is a 22.5-kDa glycoprotein used to treat diseases such as multiple sclerosis (MS). Because of appropriate post-translation modifications, protein isolation, and lack of toxicity in Chinese hamster ovary (CHO) cells, we cloned hIFNβ-1a encoding sequence into these cells by recombinant DNA technology to achieve stable expression of this recombinant protein. Methods: The hIFNβ-1a encoding sequence was designed based on the CHO cells’ codon usage and the Gene Bank data, and then syntactically constructed in the pUC57 vector. After confirmation, the synthesized sequence was cloned into the pcDNA3.1 expression vector by using EcoRI and XhoI sites via Escherichia coli DH5α competent cells. Then, the recombinant vector pcDNA-hHIFNβ1a was linearized by BglII and transfected into the CHO cells using lipofectamine. The transfected cells were proliferated and screened by gentamicin. Certain concentrations of zinc sulfate, DMSO, and glycerol were used to enhance protein expression. Finally, the recombinant protein expression was qualitatively evaluated using different techniques. Results: The hIFNβ1a integrity was confirmed by DNA sequencing and specific software. The construction and sub-cloning of hIFNβ1a-pcDNA3.1 in E. coli were confirmed by colony-PCR with specific primers and restriction enzyme mapping. The screening of transfected CHO cells was performed using gentamicin. The protein expression was confirmed by RT-PCR, MTT assay, SDS-PAGE, and Western blot. Comparison of the optimized and control samples demonstrated that chemical treatment enhanced the protein expression. Conclusion: We achieved the stable clones of CHO cells expressing the active form of human interferon beta.
{"title":"Optimization of human interferon beta protein expression in Chinese hamster ovary cells","authors":"N. Xodadadi, A. Saeidinia, M. Zeinoddini, R. Khalilzadeh","doi":"10.34172/jsums.2021.11","DOIUrl":"https://doi.org/10.34172/jsums.2021.11","url":null,"abstract":"Background and aims: Human interferon beta-1a (hIFNβ-1a) is a 22.5-kDa glycoprotein used to treat diseases such as multiple sclerosis (MS). Because of appropriate post-translation modifications, protein isolation, and lack of toxicity in Chinese hamster ovary (CHO) cells, we cloned hIFNβ-1a encoding sequence into these cells by recombinant DNA technology to achieve stable expression of this recombinant protein. Methods: The hIFNβ-1a encoding sequence was designed based on the CHO cells’ codon usage and the Gene Bank data, and then syntactically constructed in the pUC57 vector. After confirmation, the synthesized sequence was cloned into the pcDNA3.1 expression vector by using EcoRI and XhoI sites via Escherichia coli DH5α competent cells. Then, the recombinant vector pcDNA-hHIFNβ1a was linearized by BglII and transfected into the CHO cells using lipofectamine. The transfected cells were proliferated and screened by gentamicin. Certain concentrations of zinc sulfate, DMSO, and glycerol were used to enhance protein expression. Finally, the recombinant protein expression was qualitatively evaluated using different techniques. Results: The hIFNβ1a integrity was confirmed by DNA sequencing and specific software. The construction and sub-cloning of hIFNβ1a-pcDNA3.1 in E. coli were confirmed by colony-PCR with specific primers and restriction enzyme mapping. The screening of transfected CHO cells was performed using gentamicin. The protein expression was confirmed by RT-PCR, MTT assay, SDS-PAGE, and Western blot. Comparison of the optimized and control samples demonstrated that chemical treatment enhanced the protein expression. Conclusion: We achieved the stable clones of CHO cells expressing the active form of human interferon beta.","PeriodicalId":318974,"journal":{"name":"Journal of Shahrekord University of Medical Sciences","volume":"2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128247105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Najmeh Asgharzadeh, Fatemeh Hajihasani, Z. Lorigooini, M. Mardani, Hossein Amini Khoei, M. Moradi, Mehrdad Shahrani Korrani
Background and aims: Depression is one of the most common psychiatric disorders with serious impacts on individuals, and is often associated with physiological symptoms. In this study, we investigated the antidepressant effects of Kelussia odoratissima Mozaffarian extract in male mice. Methods: A total of 56 male mice (weight: 25-35 g; age: 6-8 weeks) were used. K. odoratissima Mozaffarian hydroalcoholic extract was prepared by maceration method. The forced swim test, open field test, and splash test were used to investigate the antidepressant effects. The mice were assigned into eight equal groups (n=7 each) as follows: receiving 25, 50, 75, and 100 mg/kg of K. odoratissima Mozaffarian extract; receiving 5 mg/kg reserpine; receiving 5 mg/kg reserpine along with 20 mg fluoxetine; and normal saline. All injections were done intraperitoneally for one week before the test. Malondialdehyde (MDA) levels and antioxidant capacity of serum and brain were also measured in all groups. Statistical analysis was performed by one-way ANOVA and Tukey’s test. Results: Extract of K. odoratissima Mozaffarian significantly decreased the immobility time in forced swim test (P<0.001). The extract also significantly increased splash time and elapsed time in the open field test, which was statistically significant compared with reserpinated mice (P<0.001). Reserpine increased MDA levels and decreased the antioxidant capacity of serum and brain, whereas hydroalcoholic extract of K. odoratissima decreased MDA dose-dependently and increased antioxidant capacity (P<0.001). Conclusion: The results of this study showed that hydroalcoholic extract of K. odoratissima has antidepressant effects, but further studies are necessary to investigate the involved mechanisms.
{"title":"Evaluation of antidepressant effects of hydroalcoholic extract of Kelussia odoratissima Mozaffarian in male mice","authors":"Najmeh Asgharzadeh, Fatemeh Hajihasani, Z. Lorigooini, M. Mardani, Hossein Amini Khoei, M. Moradi, Mehrdad Shahrani Korrani","doi":"10.34172/JSUMS.2021.07","DOIUrl":"https://doi.org/10.34172/JSUMS.2021.07","url":null,"abstract":"Background and aims: Depression is one of the most common psychiatric disorders with serious impacts on individuals, and is often associated with physiological symptoms. In this study, we investigated the antidepressant effects of Kelussia odoratissima Mozaffarian extract in male mice. Methods: A total of 56 male mice (weight: 25-35 g; age: 6-8 weeks) were used. K. odoratissima Mozaffarian hydroalcoholic extract was prepared by maceration method. The forced swim test, open field test, and splash test were used to investigate the antidepressant effects. The mice were assigned into eight equal groups (n=7 each) as follows: receiving 25, 50, 75, and 100 mg/kg of K. odoratissima Mozaffarian extract; receiving 5 mg/kg reserpine; receiving 5 mg/kg reserpine along with 20 mg fluoxetine; and normal saline. All injections were done intraperitoneally for one week before the test. Malondialdehyde (MDA) levels and antioxidant capacity of serum and brain were also measured in all groups. Statistical analysis was performed by one-way ANOVA and Tukey’s test. Results: Extract of K. odoratissima Mozaffarian significantly decreased the immobility time in forced swim test (P<0.001). The extract also significantly increased splash time and elapsed time in the open field test, which was statistically significant compared with reserpinated mice (P<0.001). Reserpine increased MDA levels and decreased the antioxidant capacity of serum and brain, whereas hydroalcoholic extract of K. odoratissima decreased MDA dose-dependently and increased antioxidant capacity (P<0.001). Conclusion: The results of this study showed that hydroalcoholic extract of K. odoratissima has antidepressant effects, but further studies are necessary to investigate the involved mechanisms.","PeriodicalId":318974,"journal":{"name":"Journal of Shahrekord University of Medical Sciences","volume":"21 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115777228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Fateme Sheida, Manijeh Hamzehpour, M. Sohrabi, Z. Alizadeh
Background and aims: Recently, silver nanoparticles (AgNPs) have received much attention for their possible usage in various fields. This study examined the effect of AgNPs on the histopathological changes in the prostate of rats. Methods: In this study, 40 male adult Wistar rats were divided into five equal groups (n=8 in each group). AgNPs were given orally to the four experimental groups at doses of 30, 125, 300, and 700 mg/kg for 28 consecutive days. The control group received deionized water. After performing hematoxylin and eosin (H & E) staining and Masson’s trichrome staining, the histological changes in the prostate of rats were evaluated. Results: Histological evaluation showed that the acinar epithelial height and alveolar folds decreased, but vacuoles in the epithelial cells and accumulation of blood vessel increased in the groups treated with AgNPs at doses of 30 and 125 mg/kg. The collagen content also increased significantly in these groups (30 mg/kg: P=0.03 and 125 mg/kg: P=0.002). Furthermore, the groups treated with AgNPs at doses of 300 and 700 mg/kg showed relative normalization acini and epithelial lining and the amount of their content. Conclusion: According to the results of current study, oral administration of AgNPs for 28 days had effects on prostate, indicating the toxicity of AgNPs.
{"title":"Histological modifications of the rat prostate following oral administration of silver nanoparticles","authors":"Fateme Sheida, Manijeh Hamzehpour, M. Sohrabi, Z. Alizadeh","doi":"10.34172/JSUMS.2021.08","DOIUrl":"https://doi.org/10.34172/JSUMS.2021.08","url":null,"abstract":"Background and aims: Recently, silver nanoparticles (AgNPs) have received much attention for their possible usage in various fields. This study examined the effect of AgNPs on the histopathological changes in the prostate of rats. Methods: In this study, 40 male adult Wistar rats were divided into five equal groups (n=8 in each group). AgNPs were given orally to the four experimental groups at doses of 30, 125, 300, and 700 mg/kg for 28 consecutive days. The control group received deionized water. After performing hematoxylin and eosin (H & E) staining and Masson’s trichrome staining, the histological changes in the prostate of rats were evaluated. Results: Histological evaluation showed that the acinar epithelial height and alveolar folds decreased, but vacuoles in the epithelial cells and accumulation of blood vessel increased in the groups treated with AgNPs at doses of 30 and 125 mg/kg. The collagen content also increased significantly in these groups (30 mg/kg: P=0.03 and 125 mg/kg: P=0.002). Furthermore, the groups treated with AgNPs at doses of 300 and 700 mg/kg showed relative normalization acini and epithelial lining and the amount of their content. Conclusion: According to the results of current study, oral administration of AgNPs for 28 days had effects on prostate, indicating the toxicity of AgNPs.","PeriodicalId":318974,"journal":{"name":"Journal of Shahrekord University of Medical Sciences","volume":"15 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127832944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
E. Banitalebi, Zahra Tavasoli, T. Jafari, M. Mardaniyan Ghahfarrokhi
Background and aims: The purpose of this study was to investigate the effect of eight weeks of resistance training (RT) using elastic bands together with green coffee supplement on novel hepatic steatosis biomarkers (hepatic steatosis index [HSI] and Framingham steatosis index [FSI]) in middle-aged obese women. Materials and Methods: This study was a double-blind randomized clinical trial performed on the basis of the CONSORT Statement at Shahrekord University in Iran (summer 2018). For this purpose, 60 obese women aged 30-60 years with a body mass index (BMI) of >30 kg/ m2 were selected to participate in this study and then they were randomly assigned to one of the following four groups: green coffee (GC, n=15), resistance training and placebo (RT+P, n=15), green coffee and resistance training (GC+RT, n=15), and placebo groups (P, n=15). Results: The results showed no significant differences in FSI (P=0.822) and HSI (P=0.752) between four groups. However, there were significant increases in high density lipoprotein (HDL) in the RT+P (P=0.050) and GC+RT groups (P=0.032) and significant decreases in total cholesterol (TC) in the RT+P (P=0.023), GC+RT (P=0.006), and GC groups (P=0.041). Conclusion: The reduction of hepatic steatosis biomarkers in these individuals may require a longer period of resistance workouts or other exercises.
{"title":"The effect of elastic resistance band training with green coffee supplementation on novel hepatic steatosis biomarkers in obese women: A randomized controlled trial","authors":"E. Banitalebi, Zahra Tavasoli, T. Jafari, M. Mardaniyan Ghahfarrokhi","doi":"10.34172/JSUMS.2020.30","DOIUrl":"https://doi.org/10.34172/JSUMS.2020.30","url":null,"abstract":"Background and aims: The purpose of this study was to investigate the effect of eight weeks of resistance training (RT) using elastic bands together with green coffee supplement on novel hepatic steatosis biomarkers (hepatic steatosis index [HSI] and Framingham steatosis index [FSI]) in middle-aged obese women. Materials and Methods: This study was a double-blind randomized clinical trial performed on the basis of the CONSORT Statement at Shahrekord University in Iran (summer 2018). For this purpose, 60 obese women aged 30-60 years with a body mass index (BMI) of >30 kg/ m2 were selected to participate in this study and then they were randomly assigned to one of the following four groups: green coffee (GC, n=15), resistance training and placebo (RT+P, n=15), green coffee and resistance training (GC+RT, n=15), and placebo groups (P, n=15). Results: The results showed no significant differences in FSI (P=0.822) and HSI (P=0.752) between four groups. However, there were significant increases in high density lipoprotein (HDL) in the RT+P (P=0.050) and GC+RT groups (P=0.032) and significant decreases in total cholesterol (TC) in the RT+P (P=0.023), GC+RT (P=0.006), and GC groups (P=0.041). Conclusion: The reduction of hepatic steatosis biomarkers in these individuals may require a longer period of resistance workouts or other exercises.","PeriodicalId":318974,"journal":{"name":"Journal of Shahrekord University of Medical Sciences","volume":"60 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129272869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Elham Ghorbani Jazar, Seyedeh Parisa Chavoshi Tarzjani, Z. Sadeghi, Shekoofe Alaie, S. A. Shahzadeh Fazeli
Multiple sclerosis (MS) is a chronic disease characterized by degeneration of the central nervous system (CNS). High levels of Neurofilament heavy chain (NEFH) in cerebrospinal fluid (CSF) is associated with MS. 40 MS patients and 40 controls genotyped by polymerase chain reaction (PCR) and Sanger sequencing. Genotypic and allelic distributions were compared between cases and controls. Fisher test was used to estimate the risk of MS associated with genotypes. We showed that NEFH, 1084-244G>A gene polymorphism, has no significant association with the susceptibility or severity of MS in Iranian patients (P = 0.737). Further prospective studies are required for confirmation.
{"title":"Neurofilament heavy chain gene polymorphism and risk of multiple sclerosis","authors":"Elham Ghorbani Jazar, Seyedeh Parisa Chavoshi Tarzjani, Z. Sadeghi, Shekoofe Alaie, S. A. Shahzadeh Fazeli","doi":"10.34172/JSUMS.2020.25","DOIUrl":"https://doi.org/10.34172/JSUMS.2020.25","url":null,"abstract":"Multiple sclerosis (MS) is a chronic disease characterized by degeneration of the central nervous system (CNS). High levels of Neurofilament heavy chain (NEFH) in cerebrospinal fluid (CSF) is associated with MS. 40 MS patients and 40 controls genotyped by polymerase chain reaction (PCR) and Sanger sequencing. Genotypic and allelic distributions were compared between cases and controls. Fisher test was used to estimate the risk of MS associated with genotypes. We showed that NEFH, 1084-244G>A gene polymorphism, has no significant association with the susceptibility or severity of MS in Iranian patients (P = 0.737). Further prospective studies are required for confirmation.","PeriodicalId":318974,"journal":{"name":"Journal of Shahrekord University of Medical Sciences","volume":"11 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134629555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background and aims: There are many articles about the effects of different training methods on lipid profiles in the elderly. The objective of the present study was to investigate the influence of training on the lipid profile in the elderly. Methods: In this study, databases of PubMed, Embase, Scopus, ScienceDirect, Web of Science, SID, Magiran, and Google Scholar were searched. Intervention effects were presented as mean difference (MD) with a random-effects model. Subgroup analysis and sensitivity analysis were performed to study heterogeneity, following the primary screening of the full text of the articles. Results: A total of 23 trials with 1654 individuals were included in the meta-analysis. Levels of high-density lipoprotein cholesterol (HDL-C) (MD = 0.47 mg/dL; P<0.001, SE = 0.08, V = 0.01, 95% CI = 0.31 0.63, Z = 5.73, 19 trials), low-density lipoprotein cholesterol (LDL-C) (MD = -0.46; P<0.001, SE = 0.11, V = 0.01, 95% CI = -0.68 -0.25, Z = -4.24), triglyceride (MD = -0.62; P=0.001, SE = 0.12, V = 0.01, 95% CI = -0.86 -0.38, Z = -5.03, 20 trials), and total cholesterol (TC) (MD = -0.33; P<0.001, SE = 0.09, V = 0.01, 95% CI = -0.52 -0.15, Z = -3.57, 16 trials) were investigated. Following sensitivity analysis and heterogeneity testing, the results were still strong and impressive. Conclusion: Lipid profiles improved in training groups, indicating higher levels of HDL-C and lower levels of LDL-C, TC, and triglyceride. Overall, training leads to a better lipid profile. However, closer scrutiny seems necessary.
背景与目的:关于不同训练方法对老年人血脂的影响的文章很多。本研究的目的是探讨训练对老年人血脂的影响。方法:检索PubMed、Embase、Scopus、ScienceDirect、Web of Science、SID、Magiran、Google Scholar等数据库。干预效果用随机效应模型表示为平均差值(MD)。在对文章全文进行初步筛选后，进行亚组分析和敏感性分析以研究异质性。结果:荟萃分析共纳入23项试验，涉及1654名个体。高密度脂蛋白胆固醇(HDL-C)水平(MD = 0.47 mg/dL;P<0.001, SE = 0.08, V = 0.01, 95% CI = 0.31 0.63, Z = 5.73, 19项试验)，低密度脂蛋白胆固醇(LDL-C) (MD = -0.46;P<0.001, SE = 0.11, V = 0.01, 95% CI = -0.68 -0.25, Z = -4.24)，甘油三酯(MD = -0.62;P=0.001, SE = 0.12, V = 0.01, 95% CI = -0.86 -0.38, Z = -5.03, 20项试验)和总胆固醇(TC) (MD = -0.33;P<0.001, SE = 0.09, V = 0.01, 95% CI = -0.52 -0.15, Z = -3.57，共16项试验)。经过敏感性分析和异质性检验，结果仍然是强有力的和令人印象深刻的。结论:脂质谱在训练组中有所改善，表明HDL-C水平较高，LDL-C、TC和甘油三酯水平较低。总的来说，训练可以改善血脂。然而，更仔细的审查似乎是必要的。
{"title":"Effect of exercise training on lipid profiles in the elderly: A systematic review and meta-analysis of randomized controlled trials","authors":"M. Ghafari, M. Faramarzi, Timoty Baghurst","doi":"10.34172/JSUMS.2020.32","DOIUrl":"https://doi.org/10.34172/JSUMS.2020.32","url":null,"abstract":"Background and aims: There are many articles about the effects of different training methods on lipid profiles in the elderly. The objective of the present study was to investigate the influence of training on the lipid profile in the elderly. Methods: In this study, databases of PubMed, Embase, Scopus, ScienceDirect, Web of Science, SID, Magiran, and Google Scholar were searched. Intervention effects were presented as mean difference (MD) with a random-effects model. Subgroup analysis and sensitivity analysis were performed to study heterogeneity, following the primary screening of the full text of the articles. Results: A total of 23 trials with 1654 individuals were included in the meta-analysis. Levels of high-density lipoprotein cholesterol (HDL-C) (MD = 0.47 mg/dL; P<0.001, SE = 0.08, V = 0.01, 95% CI = 0.31 0.63, Z = 5.73, 19 trials), low-density lipoprotein cholesterol (LDL-C) (MD = -0.46; P<0.001, SE = 0.11, V = 0.01, 95% CI = -0.68 -0.25, Z = -4.24), triglyceride (MD = -0.62; P=0.001, SE = 0.12, V = 0.01, 95% CI = -0.86 -0.38, Z = -5.03, 20 trials), and total cholesterol (TC) (MD = -0.33; P<0.001, SE = 0.09, V = 0.01, 95% CI = -0.52 -0.15, Z = -3.57, 16 trials) were investigated. Following sensitivity analysis and heterogeneity testing, the results were still strong and impressive. Conclusion: Lipid profiles improved in training groups, indicating higher levels of HDL-C and lower levels of LDL-C, TC, and triglyceride. Overall, training leads to a better lipid profile. However, closer scrutiny seems necessary.","PeriodicalId":318974,"journal":{"name":"Journal of Shahrekord University of Medical Sciences","volume":"47 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122659808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：