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Characteristics of seaweed caraginan Kappaphycus alvarezii on cultivation system with different seed weight 不同种子重量栽培系统上的海藻卡拉胶 Kappaphycus alvarezii 的特征
Pub Date : 2024-02-13 DOI: 10.19027/jai.23.1.71-78
Muhammad Rasnijal, Ardana Kurniaji, Anton Anton, Budiyati Budiyati, Diana Putri Renitasari, Achmad Suhermanto, Mugi Mulyono, I. S. Djunaidah, Sinung Rahardjo, Sinar Pagi Sektiana, Ridwan Ridwan
Seaweed (Kappaphycus alvarezii) could be a source of carrageenan needed for industrial sector. The purpose of this study to analyze the characteristics of carrageenan seaweed cultivated with bag and non-bag cultivation systems and different seed weights. The research was conducted in the waters of Bone Bay and the Laboratory of the Indonesian Center for Brackish Water Cultivation and Fisheries Extension in Maros. This study used a factorial completely randomized design (CRD) consisting of factor A (using bags and non-bags), and factor B (seed weight 15, 50, 75, 100, and 125 g). The results showed that the cultivation method factor, seed weight factor, and their interaction had an effect on carrageenan yield, gel strength, ash content and water content (P <0.05). Different cultivation systems with different initial seed weight combinations showed different best results for each parameter. The highest value of yield of carrageenan was observed in the bag culture system with a seed weight of 100 g, namely 29.18 ± 1.10%. The highest value of gel strength was observed in the non-bagged cultivation system with a seed weight of 75 g, namely 1344.69 ± 18.43 g/cm2. The highest value of ash content was found in the non-bagged cultivation system with 125 g of seed weight, namely 30.02 ± 0.13%. The highest value of water content was found in the bag culture system at a seed weight of 15 g, namely 38.63 ± 0.26%. Different cultivation methods and seed weight resulted in other carrageenan characteristics of seaweed for each parameter.   Keywords: gel strength, ash content, moisture content, yield   ABSTRAK   Rumput laut (Kappaphycus alvarezii) memiliki potensi sebagai sumber karaginan yang banyak dibutuhkan untuk bidang industri. Penelitian ini bertujuan untuk menganalisis karakteristik karaginan rumput laut yang dibudidayakan dengan sistem budidaya kantong dan non kantong serta berat bibit yang berbeda. Penelitian dilaksanakan di perairan Teluk Bone dan Laboratorium Balai Riset Budidaya Air Payau dan Penyuluhan Peikanan Maros. Percobaan penelitian dilakukan melalui rancangan acak lengkap (RAL) faktorial yang terdiri faktor A (memakai kantong dan non kantong), faktor B (berat bibit 15, 50, 75, 100, dan 125 g). Hasil penelitian menunjukkan bahwa faktor metode budidaya, berat bibit dan interaksi keduanya berpengaruh terhadap rendeman karaginan, kekuatan gel, kadar air, kadar abu (P<0,05). Penggunaan sistem budidaya yang berbeda dengan kombinasi berat awal bibit berbeda menunjukkan hasil terbaik yang tidak sama pada tiap parameter. Nilai tertinggi rendeman keraginan teramati pada sistem budidaya kantong dengan berat bibit 100 g yakni 29,18 ± 1,10 %. Nilai tertinggi kekuatan gel teramati pada sistem budidaya non kantong dengan berat bibit 50 g yaitu 1344,69 ± 18,43 g/cm2. Nilai kadar abu tertinggi pada sistem budidaya non kantong dengan berat bibit 125 g yaitu 30,02 ± 0,13%. Nilai kadar air tertinggi pada sistem budidaya kantong dengan berat bibit 15 g yaitu
海藻(Kappaphycus alvarezii)可作为工业部门所需的卡拉胶来源。本研究的目的是分析用袋式和非袋式栽培系统以及不同种子重量栽培的卡拉胶海藻的特性。研究在马罗斯的骨湾水域和印度尼西亚咸水养殖和渔业推广中心实验室进行。该研究采用了因子完全随机设计(CRD),包括因子 A(使用袋和不使用袋)和因子 B(种子重量 15、50、75、100 和 125 克)。结果表明,栽培方法因子、种子重量因子及其交互作用对卡拉胶产量、凝胶强度、灰分含量和含水量均有影响(P <0.05)。不同的栽培系统和不同的初始种子重量组合对各参数都显示出不同的最佳结果。在种子重量为 100 克的袋栽培系统中,卡拉胶产量最高,为 29.18 ± 1.10%。种子重量为 75 克的非袋装栽培系统的凝胶强度值最高,为 1344.69 ± 18.43 克/平方厘米。种子重量为 125 克的非袋装栽培系统的灰分含量最高,为 30.02 ± 0.13%。在种子重量为 15 克的袋栽培系统中,水分含量最高,为 38.63 ± 0.26%。不同的培养方法和种子重量导致海藻的卡拉胶特性各不相同。 关键词:凝胶强度、灰分含量、水分含量、产量 ABSTRAK Rumput laut (Kappaphycus alvarezii) memiliki potensi sebagai sumber karaginan yang banyak dibutuhkan untuk bidang industri.它的作用是管理从传统和非传统的植物系统中分离出来的茎叶植物,以及植物的茎叶。在特卢克骨和巴尤岛空气污染与马罗斯山脉空气污染实验室进行了检测。通过 A 项(记住重量和不记住重量)、B 项(重量分别为 15、50、75、100 和 125 克)的 RAL(Rancangan acak lengkap)实验来确定检测项目。实验结果表明,芽苞模式、茎秆重量和茎秆间距对茎秆重量、凝胶重量、空气重量和茎秆重量的影响均小于 0.05(P<0.05)。与其他参数相比,该系统具有更高的可靠性。100 克体重的芽孔系统的平均畸变率为 29.18 ± 1.10 %。在 50 克重的非肛门系统中,凝胶的凝结率为 1344.69 ± 18.43 克/平方厘米。在 125 克重的非悬挂式喷射系统中,空气中的凝胶含量为 30.02 ± 0.13%。在 15 克重的芽苞生长系统中,空气的比例为 38.63 ± 0.26%。新陈代谢法和掺入量的计算显示,在不同的参数下,新陈代谢法和掺入量的计算显示不同的新陈代谢法和掺入量的计算显示不同的新陈代谢法和掺入量的计算显示不同的新陈代谢法。 关键字:凝胶、空气、渲染
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引用次数: 0
Evaluation of Cinnamaldehyde Administration to Feed with Different Protein Energy Levels and Ratios to Pacific whiteleg Shrimp (Litopenaeus vannamei) 在太平洋南美白对虾(Litopenaeus vannamei)不同蛋白质能量水平和比例的饲料中添加肉桂醛的评估
Pub Date : 2024-02-13 DOI: 10.19027/jai.23.1.79-91
A. Hendriana, M. Setiawati, D. Jusadi, M. Suprayudi, J. Ekasari, D. Wahjuningrum
This study aims to evaluate the growth performance and carbohydrate metabolism of Pacific whiteleg shrimp Litopenaeus vannamei given cinnamaldehyde levels in protein and different feed energy ratios. The study used a completely randomized design (CRD) with six treatments and three replications. The treatment consisted of control treatment C013 (32% protein feed with C/P of 13 without the addition of cinnamaldehyde), treatment C0.0513 (32% protein feed with 13 C/P added 0.05% of cinnamaldehyde), C0.0514 (28% protein feed with 14 C/P added of 0.05% cinnamaldehyde), C0.114 (28% protein feed with C/P of 14 added of 0.10% cinnamaldehyde), C0.0515 treatment (28% protein feed with 15 C/P added of 0.05% cinnamaldehyde), and 0.115 C treatment (28% protein feed with 15 C/P added of 0.10% cinnamaldehyde). The study was conducted for 56 days of maintenance in a 76 L volume aquarium using Pacific whiteleg shrimp measuring 1.38 ± 0.01 g at a density of 15 individuals per aquarium. The results showed that the C0.0513 treatment produced growth performance, including the final average weight and specific growth rate (SGR), which were significantly different (P<0.05) and resulted in protein retention, lipid retention, and feed conversion ratio (FCR) which were higher than the control treatment (C013). In addition, the highest parameters of hexokinase (hk) and phosphoenolpyruvate carboxykinase (pepck) were produced by treatment C0.0513 and significantly different (P<0.05) from the control treatment (C013). This study showed that the C0.0513 treatment was the best non-protein energy source to increase growth performance through carbohydrate metabolism in Pacific whiteleg shrimp.   Keyword: Carbohydrate metabolism, growth, feed energy ratio, cinnamaldehyde, Pacific whiteleg shrimp   ABSTRAK   Penelitian ini bertujuan untuk mengevaluasi kinerja pertumbuhan dan metabolisme karbohidrat udang vaname Litopenaeus vannamei yang diberikan kadar sinamaldehid pada protein dan rasio energi pakan berbeda.  Penelitian menggunakan Rancangan Acak Lengkap (RAL) dengan enam perlakuan dan tiga ulangan.  Adapun perlakuan terdiri dari perlakuan  kontrol C013 (pakan protein 32% dengan C/P sebesar 13 tanpa penambahan sinamaldehid), perlakuan C0.0513 (pakan protein 32% dengan C/P sebesar 13 ditambahakan sinamaldehid sebesar 0.05%), perlakuan C0.0514 (pakan protein 28% dengan C/P sebesar 14 ditambahakan sinamaldehid sebesar 0.05%), perlakuan C0.114 (pakan protein 28% dengan C/P sebesar 14 ditambahakan sinamaldehid sebesar 0.10%), perlakuan C0.0515 (pakan protein 28% dengan C/P sebesar 15 ditambahakan sinamaldehid sebesar 0.05%), dan perlakuan C 0.115 (pakan protein 28% dengan C/P sebesar 15 ditambahakan sinamaldehid sebesar 0.10%). Penelitian dilakukan selama 56 hari pemeliharaan pada akuarium volume 76 L menggunakan udang vaname berukuran 1.38 ± 0.01 g dengan kepadatan 15 ekor per akuarium. Hasil penelitian menunjukkan bahwa perlakuan C0.0513 menghasilkan kinerja pertumbuhan meliputi
本研究旨在评估太平洋南美白对虾(Litopenaeus vannamei)在肉桂醛蛋白质水平和不同饲料能量比条件下的生长性能和碳水化合物代谢。研究采用完全随机设计(CRD),六个处理三个重复。处理包括对照处理 C013(32% 蛋白质饲料,C/P 为 13,不添加肉桂醛)、处理 C0.0513(32% 蛋白质饲料,C/P 为 13,添加 0.05% 肉桂醛)、处理 C0.0514(28% 蛋白质饲料,C/P 为 14,添加 0.05%肉桂醛)、C0.114(28%蛋白质饲料,14C/P添加0.10%肉桂醛)、C0.0515处理(28%蛋白质饲料,15C/P添加0.05%肉桂醛)和0.115C处理(28%蛋白质饲料,15C/P添加0.10%肉桂醛)。该研究在一个容积为 76 L 的水族箱中进行了 56 天的养护,使用的是规格为 1.38 ± 0.01 g 的太平洋白对虾,密度为每个水族箱 15 只。结果表明,C0.0513 处理产生的生长性能,包括最终平均体重和特定生长率(SGR),与对照处理(C013)相比有显著差异(P<0.05),并导致蛋白质保留率、脂质保留率和饲料转化率(FCR)更高。此外,C0.0513 处理产生的己糖激酶(hk)和磷酸烯醇丙酮酸羧激酶(pepck)参数最高,与对照处理(C013)相比差异显著(P<0.05)。该研究表明,C0.0513 处理是通过碳水化合物代谢提高太平洋白对虾生长性能的最佳非蛋白质能量来源。 关键词:碳水化合物代谢 生长 饲料能量比 肉桂醛 太平洋白对虾 ABSTRAK Penelitian ini bertujuan untuk mengevaluasi kinerja pertumbuhan and metabolisme karbohidrat udang vaname Litopenaeus vannamei yang diberikan kadar sinamaldehid pada protein and rasio energi pakan berbeda. 这种鱼类可通过藻类和大型藻类的适应性来提高藻类生长速度(RAL)。 对照组 C013(蛋白质含量为 32%,C/P 为 13,蛋白质含量为 0.0513(蛋白质含量为 32%,C/P 为 13,蛋白质含量为 0.0513),对照组 C0.0513(蛋白质含量为 32%,C/P 为 13,蛋白质含量为 0.0513),对照组 C0.0513(蛋白质含量为 32%,C/P 为 13,蛋白质含量为 0.0513)。0513(蛋白质含量为 32%,C/P 含量为 13,而 sinamaldehid 含量为 0.05%)、C0.0514(蛋白质含量为 28%,C/P 含量为 14,而 sinamaldehid 含量为 0.05%)、C0.0513(蛋白质含量为 32%,C/P 含量为 13,而 sinamaldehid 含量为 0.05%)、C0.0514(蛋白质含量为 28%,C/P 含量为 14,而 sinamaldehid 含量为 0.05%), perlakuan C0.114 (pakan protein 28% dengan C/P sebesar 14 ditambahakan sinamaldehid sebesar 0.10%), perlakuan C0.0515(蛋白质含量为 28%,C/P 含量为 15,膳食纤维含量为 0.05%)和 C 0.115(蛋白质含量为 28%,C/P 含量为 15,膳食纤维含量为 0.10%)。56天后,在容积为76升的池塘中生长的鱼类每池可产1.38(± 0.01)克鳗鱼,每池可产15克鳗鱼。试验结果表明,C0.0513 的饲养条件能显著提高饲料转化率(FCR)和特定生长率(SGR)(P<0.05),同时与对照饲养条件(C013)相比,蛋白质回收率、脂肪回收率和饲料转化率(FCR)也有所提高。此外,褐藻糖激酶(hk)和磷酸烯醇丙酮酸羧激酶(pepck)参数在对照组(C013)中的平均值为 0.0513,而在对照组(C013)中的平均值为 P<0.05。该研究表明,C0.0513 是非蛋白质能量总和的主要来源,它可以通过万年青的新陈代谢产生能量。 相关资料:胰岛素代谢、胰岛素、胰岛素胰岛素、胰岛素胰岛素、胰岛素胰岛素
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引用次数: 0
Evaluation of Cinnamaldehyde Administration to Feed with Different Protein Energy Levels and Ratios to Pacific whiteleg Shrimp (Litopenaeus vannamei) 在太平洋南美白对虾(Litopenaeus vannamei)不同蛋白质能量水平和比例的饲料中添加肉桂醛的评估
Pub Date : 2024-02-13 DOI: 10.19027/jai.23.1.79-91
A. Hendriana, M. Setiawati, D. Jusadi, M. Suprayudi, J. Ekasari, D. Wahjuningrum
This study aims to evaluate the growth performance and carbohydrate metabolism of Pacific whiteleg shrimp Litopenaeus vannamei given cinnamaldehyde levels in protein and different feed energy ratios. The study used a completely randomized design (CRD) with six treatments and three replications. The treatment consisted of control treatment C013 (32% protein feed with C/P of 13 without the addition of cinnamaldehyde), treatment C0.0513 (32% protein feed with 13 C/P added 0.05% of cinnamaldehyde), C0.0514 (28% protein feed with 14 C/P added of 0.05% cinnamaldehyde), C0.114 (28% protein feed with C/P of 14 added of 0.10% cinnamaldehyde), C0.0515 treatment (28% protein feed with 15 C/P added of 0.05% cinnamaldehyde), and 0.115 C treatment (28% protein feed with 15 C/P added of 0.10% cinnamaldehyde). The study was conducted for 56 days of maintenance in a 76 L volume aquarium using Pacific whiteleg shrimp measuring 1.38 ± 0.01 g at a density of 15 individuals per aquarium. The results showed that the C0.0513 treatment produced growth performance, including the final average weight and specific growth rate (SGR), which were significantly different (P<0.05) and resulted in protein retention, lipid retention, and feed conversion ratio (FCR) which were higher than the control treatment (C013). In addition, the highest parameters of hexokinase (hk) and phosphoenolpyruvate carboxykinase (pepck) were produced by treatment C0.0513 and significantly different (P<0.05) from the control treatment (C013). This study showed that the C0.0513 treatment was the best non-protein energy source to increase growth performance through carbohydrate metabolism in Pacific whiteleg shrimp.   Keyword: Carbohydrate metabolism, growth, feed energy ratio, cinnamaldehyde, Pacific whiteleg shrimp   ABSTRAK   Penelitian ini bertujuan untuk mengevaluasi kinerja pertumbuhan dan metabolisme karbohidrat udang vaname Litopenaeus vannamei yang diberikan kadar sinamaldehid pada protein dan rasio energi pakan berbeda.  Penelitian menggunakan Rancangan Acak Lengkap (RAL) dengan enam perlakuan dan tiga ulangan.  Adapun perlakuan terdiri dari perlakuan  kontrol C013 (pakan protein 32% dengan C/P sebesar 13 tanpa penambahan sinamaldehid), perlakuan C0.0513 (pakan protein 32% dengan C/P sebesar 13 ditambahakan sinamaldehid sebesar 0.05%), perlakuan C0.0514 (pakan protein 28% dengan C/P sebesar 14 ditambahakan sinamaldehid sebesar 0.05%), perlakuan C0.114 (pakan protein 28% dengan C/P sebesar 14 ditambahakan sinamaldehid sebesar 0.10%), perlakuan C0.0515 (pakan protein 28% dengan C/P sebesar 15 ditambahakan sinamaldehid sebesar 0.05%), dan perlakuan C 0.115 (pakan protein 28% dengan C/P sebesar 15 ditambahakan sinamaldehid sebesar 0.10%). Penelitian dilakukan selama 56 hari pemeliharaan pada akuarium volume 76 L menggunakan udang vaname berukuran 1.38 ± 0.01 g dengan kepadatan 15 ekor per akuarium. Hasil penelitian menunjukkan bahwa perlakuan C0.0513 menghasilkan kinerja pertumbuhan meliputi
本研究旨在评估太平洋南美白对虾(Litopenaeus vannamei)在肉桂醛蛋白质水平和不同饲料能量比条件下的生长性能和碳水化合物代谢。研究采用完全随机设计(CRD),有六个处理和三次重复。处理包括对照处理 C013(32% 蛋白质饲料,C/P 为 13,不添加肉桂醛)、处理 C0.0513(32% 蛋白质饲料,C/P 为 13,添加 0.05% 肉桂醛)、处理 C0.0514(28% 蛋白质饲料,C/P 为 14,添加 0.05%肉桂醛)、C0.114(28%蛋白质饲料,14C/P添加0.10%肉桂醛)、C0.0515处理(28%蛋白质饲料,15C/P添加0.05%肉桂醛)和0.115C处理(28%蛋白质饲料,15C/P添加0.10%肉桂醛)。该研究在一个容积为 76 L 的水族箱中进行了 56 天的养护,使用的是规格为 1.38 ± 0.01 g 的太平洋白对虾,密度为每个水族箱 15 只。结果表明,C0.0513 处理产生的生长性能,包括最终平均体重和特定生长率(SGR),与对照处理(C013)相比有显著差异(P<0.05),并导致蛋白质保留率、脂质保留率和饲料转化率(FCR)更高。此外,C0.0513 处理产生的己糖激酶(hk)和磷酸烯醇丙酮酸羧激酶(pepck)参数最高,与对照处理(C013)相比差异显著(P<0.05)。该研究表明,C0.0513处理是通过碳水化合物代谢提高太平洋白对虾生长性能的最佳非蛋白能源。 关键词碳水化合物代谢 生长 饲料能量比 肉桂醛 太平洋南美白对虾 ABSTRACT This study aimed to evaluate the growth performance and carbohydrate metabolism of Litopenaeus vannamei vaname shrimp given cinnamaldehyde levels at different protein and feed energy ratios. 该研究采用完全随机设计(CRD),有六个处理和三个重复。 05%)、处理 C0.114(28% 蛋白质饲料,C/P 为 14 加 0.10%的窦甲醛)、处理 C0.0515(28% 蛋白质饲料,C/P 为 15 加 0.05%的窦甲醛)和处理 C0.115(28% 蛋白质饲料,C/P 为 15 加 0.10%的窦甲醛)。该研究在一个 76 L 的水族箱中饲养了 56 天,使用的是规格为 1.38 ± 0.01 g 的凡纳滨对虾,密度为每个水族箱 15 尾。结果表明,与对照处理(C013)相比,C0.0513 处理的最终平均体重和特定生长率(SGR)有显著差异(P<0.05),并产生了更高的蛋白质保留率、脂肪保留率和饲料转化率(FCR)。此外,C0.0513 处理产生的六磷酸酶(hk)和磷酸烯醇丙酮酸羧激酶(pepck)参数最高,与对照处理(C013)相比差异显著(P<0.05)。本研究表明,C0.0513 处理是通过碳水化合物代谢改善凡纳滨对虾生长性能的最佳非蛋白能源处理。 关键词:碳水化合物代谢 生长 饲料能量比 sinamaldehyde 香芒对虾
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引用次数: 0
Evaluation of single and multispecies probiotic applications for the prevention of Motile Aeromonads Septicaemia in gourami, Osphronemus gourami 评估单一和多物种益生菌应用于预防鮨科鱼类动气单胞菌败血症的效果
Pub Date : 2024-01-26 DOI: 10.19027/jai.23.1.56-70
Agista Tsani Untsa, W. Widanarni, Sukenda, Usamah Afiff, M. Yuhana
The aim of this study was to evaluate single and multispecies probiotic applications for the prevention of Motile Aeromonads Septicaemia (MAS) disease in gourami, Osphronemus gourami. The experiment consisted of the in vitro inhibition test and the in vivo application of probiotics in gourami. The in vivo assay, consisted of five treatments and five replicates, namely: negative control (K-); positive control (K+); (B) fish fed with  supplementation of 1% (v/w) probiotic Bacillus NP5 RifR cells and challenged with Aeromonas hydrophila RifR; (L) fish fed with supplemention of probiotic Lactobacillus plantarum CipR 1% (v/w); challenged with A. hydrophila RifR; (BL) fish fed with mixture supplementation of Bacillus NP5 RifR 0.5% (v/w)+L. plantarum CipR 0.5%; infected with A. hydrophila RifR. Fish (29.57 ± 1.00 g) were reared in a 45 L volume aquaria with a rearing density of 10 fish/m3 for 50 days, with   feeding trial three times a day. The challenge test was conducted by intramuscular injection with pathogenic A. hydrophila RifR cells (106 CFU/mL) on day 41st. In vitro test results showed that single and multispecies probiotics significantly (P<0.05) inhibited A.hydrophila RifR. In vivo experiment showed that probiotic supplementation treatments improved the growth performance, and microbiota diversity in the gut.  The immune responses, fish resistance to A. hydrophila RifR and gourami survival rate in all treatments of supplemented feed were significantly higher compared to the positive control. The best treatment, multispecies probiotics significantly (P<0.05) improved the survival of gourami 96.67% post infection with A. hydrophila RifR.   Keywords: A. hydrophila, Bacillus NP5, L. plantarum, O. gourami, probiotic   ABSTRAK   Penelitian ini bertujuan untuk mengevaluasi aplikasi probiotik tunggal dan multispesies dalam pencegahan penyakit Motile Aeromonad Septicaemia (MAS) yang disebabkan oleh Aeromonas hydrophila pada ikan gurami (Osphronemus gourami). Penelitian terdiri atas uji penghambatan in vitro bakteri probiotik tunggal dan multispesies terhadap A. hydrophila RifR, dan uji in vivo aplikasi probiotik untuk gurami. Uji in vivo, terdiri dari lima perlakuan dan lima ulangan, yaitu: kontrol negatif (K-) kontrol positif (K+); (B) ikan dengan pakan suplementasi Bacillus NP5 RifR 1% dan diinfeksi A. hydrophila RifR; (L) pakan komersial dengan penambahan probiotik L. plantarum CipR 1% dan infeksi A. hydrophila RifR; (BL) ikan dengan suplementasi campuran Bacillus NP5 RifR 0.5% dan L. plantarum CipR 0.5% serta diinfeksi A. hydrophila RifR. Benih gurami (29.57 ± 1.00 g) dipelihara di dalam akuarium bervolume 45 L dengan kepadatan 10 ekor/m3 selama 50 hari, dengan pakan bersuplemen probiotik 3 kali sehari. Uji tantang dilakukan dengan menginjeksikan suspensi sel patogen A. hydrophila RifR (106 CFU/mL) secara intramuscular pada hari ke 41. Hasil uji in vitro menunjukkan bahwa probiotik tunggal dan multispesies dapat menghambat pertumbuhan sel A.h
本研究的目的是评估单一物种和多物种益生菌在预防口蹄鲉运动性气单胞菌败血症(MAS)方面的应用。实验包括体外抑制试验和体内应用益生菌。体内试验包括五个处理和五个重复,即:阴性对照组(K-);阳性对照组(K+);(B)喂食 1%(体积分数)益生菌芽孢杆菌 NP5 RifR 细胞并受到嗜水气单胞菌 RifR 挑战的鱼;(L)喂食 1%(体积分数)益生菌植物乳杆菌 CipR 并受到嗜水气单胞菌 RifR 挑战的鱼;(A)喂食 1%(体积分数)益生菌芽孢杆菌 NP5 RifR 细胞并受到嗜水气单胞菌 RifR 挑战的鱼;(B)喂食 1%(体积分数)益生菌芽孢杆菌 NP5 RifR 细胞并受到嗜水气单胞菌 RifR 挑战的鱼。(L)喂食益生菌植物乳杆菌 CipR 1%(体积分数)的鱼;感染蚜虫 RifR;(BL)喂食 NP5 芽孢杆菌 RifR 0.5%(体积分数)+植物乳杆菌 CipR 0.5%(体积分数)的鱼;感染蚜虫 RifR。鱼(29.57 ± 1.00 g)在容积为 45 L 的水族箱中饲养 50 天,饲养密度为 10 尾/立方米,每天喂食三次。第 41 天,通过肌肉注射致病性姬蛙 RifR 细胞(106 CFU/mL)进行挑战试验。体外试验结果表明,单一益生菌和多菌种益生菌对蚜虫 RifR 有明显的抑制作用(P<0.05)。体内实验表明,补充益生菌能改善鱼的生长性能和肠道微生物群的多样性。 与阳性对照相比,所有添加益生菌的饲料处理的免疫反应、鱼类对蚜虫 RifR 的抵抗力和小龙虾存活率都显著提高。最佳处理多物种益生菌能明显提高(P<0.05)感染虹彩嗜水性蛛形纲RifR后东方豚的存活率96.67%。 关键词嗜水气单胞菌;NP5芽孢杆菌;植物乳杆菌;O. gourami;益生菌 ABSTRAK Penelitian ini bertujuan untuk mengevaluasi aplikasi probiotik tunggal and multispesies dalam pencegahan penyakit Motile Aeromonad Septicaemia (MAS) yang disebabkan oleh Aeromonas hydrophila pada ikan gurami (Osphronemus gourami)。该项目包括体外对嗜水气单胞菌 RifR 和体内益生菌对嗜水气单胞菌的增殖和多型性的研究。活体益生菌包括:阴性对照组(K-)和阳性对照组(K+);(B)添加 1%的 NP5 RifR 杆菌并同时添加 A. hydrophila RifR;(C)添加 1%的 NP5 RifR 杆菌并同时添加 A. hydrophila RifR;(D)添加 1%的 NP5 RifR 杆菌并同时添加 A. hydrophila RifR;(E)添加 1%的 NP5 RifR 杆菌并同时添加 A. hydrophila RifR。水虱 RifR;(L) 以植物酵母菌 CipR 1% 和水虱 RifR 为增殖益生菌的水生动物组;(BL) 以 NP5 杆菌 RifR 0.5% 和植物酵母菌 CipR 0.5% 为增殖益生菌的水生动物组,并以水虱 RifR 为增殖益生菌的水生动物组。在 50 个月后,在容积为 45 升、每立方米 10 微克的水池中添加了益生菌(29.57 ± 1.00 克),自此以后每年添加 3 次益生菌。41 天内,肌肉注射病原嗜水杆 菌 RifR 悬浮液(106 CFU/mL),以稀释微生物。体外实验结果表明,嗜水木虱 RifR 的多型性和多型性益生菌能显著提高嗜水木虱 RifR 在人体器官和人参中的浓度(P<0.05)。活体研究显示,大型益生菌和多型益生菌可提高藻类的抗病能力和微生物群的数量。水飞蓟马对 RifR 的耐受性和对水飞蓟马的耐受性与益生菌的作用是成正比的。 多型益生菌对嗜水蝇 RifR 的影响显著(P<0.05),达到 96.67%。 Kata kunci: A. hydrophila, Bacillus sp. NP5, L. plantarum, O. goramy, probiotik
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引用次数: 0
The The effectiveness of galangal (Alpinia galanga) extract for the treatment of sangkuriang catfish (Clarias gariepinus) infected Aeromonas hydrophila 高良姜(Alpinia galanga)提取物治疗受嗜水气单胞菌感染的山瑞鲶(Clarias gariepinus)的有效性
Pub Date : 2024-01-26 DOI: 10.19027/jai.23.1.44-55
Yuli Andriani, Desy Setyo Wati, I. Zidni, Roffi Grandiosa Herman
The purpose of this research is to find out the optimal dose of galangal extract to treatment of sangkuriang catfish infected with Aeromonas hydrophila, the fish fry used are 5-7 cm in size. October-November 2022 was the time the research was carried out. The experimental method used in this study was a completely randomized design (CRD) with 4 treatments and 3 replications. The treatment used was immersing with galangal extract for 24 hours with doses of 0 mg/L (A), 250 mg/L (B), 500 mg/L (C) and 750 mg/L (D). Maintenance of test fish for 14 days. Aeromonas hydrophila bacteria are used to infect fish with a density of 108 CFU/ml by immersing 10 ml in 10 liters of water. Clinical symptoms of infection, clinical symptoms of recovery period, survival rate and water quality were used as parameters in this research. The results showed that the use of galangal extract for treatment of sangkuriang catfish fry infected with Aeromonas hydrophila by immersion method for 24 hours at a concentration of 500 mg/L was the best treatment with the survival rate of 90%.
本研究的目的是找出高良姜提取物治疗感染嗜水气单胞菌的桑库良鲶鱼的最佳剂量,所用鱼苗的大小为 5-7 厘米。研究时间为 2022 年 10 月至 11 月。本研究采用的实验方法是完全随机设计(CRD),有 4 个处理和 3 次重复。处理方法是将高良姜提取物浸泡 24 小时,剂量分别为 0 毫克/升(A)、250 毫克/升(B)、500 毫克/升(C)和 750 毫克/升(D)。试验鱼维持 14 天。将嗜水气单胞菌以 108 CFU/ml 的密度感染鱼类,方法是将 10 毫升浸入 10 升水中。感染的临床症状、恢复期的临床症状、存活率和水质被作为本研究的参数。结果表明,使用高良姜提取物治疗感染嗜水气单胞菌的桑吉祥鲶鱼苗,浸泡法24小时,浓度为500毫克/升,是最佳治疗方法,存活率为90%。
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引用次数: 0
The presence of disease in growing spiny lobster Panulirus spp. in floating net cages at Tanjung Putus Island, Lampung 楠榜丹绒普陀斯岛浮动网箱中生长的棘龙虾(Panulirus spp.
Pub Date : 2024-01-12 DOI: 10.19027/jai.23.1.24-35
S. Sukenda, Mira Lastriliah, W. Widanarni, Irzal Efendi, Dendi Hidayatullah
Spiny lobster is a member of crustaceans that has high economic value. The demand for export markets in Asian, European and American countries as well as locally, is quite high with an expensive selling price. However, there are obstacles that often occur in spiny lobster rearing cultivation, namely infectious diseases that will cause high mortality. The purpose of this study was to investigate infectious diseases in rearing spiny lobster Panulirus spp. that were kept in floating net cages at Tanjung Putus, Lampung. The research method involved sampling five spiny lobsters in floating net cages, observation of clinical symptoms, bacterial isolation, identification of bacteria, detection of Milky Hemolymph Disease of Spiny Lobster (MHD-SL) through polymerase chain reaction (PCR) analysis and histopathology. The sampling location was at PT. Saibatin Perikanan Indonesia. The bacteria Vibrio parahaemolyticus and Vibrio alginolyticus were detected. Five lobsters were infected by MHD-SL, characterized by milky white hemolymph. All five lobster samples were positively infected with Milky Hemolymph Disease of Spiny Lobster (MHD-SL) through PCR analysis. Histopathological observations showed pathological microanatomical changes in the lobster hepatopancreas tissue, indicating that the tissue changes, in the form of encapsulation and infiltration were due to Rickettsia-like bacteria (RLB) infection. The management of spiny lobster stocking density, maintenance biosecurity, and increased lobster immunity must be implemented in order to prevent MHD-SL disease in floating net cages.   Keywords: bacteria, histopathology, MHD-SL, Panulirus spp.   ABSTRAK   Lobster merupakan anggota crustacea yang memiliki nilai ekonomis yang tinggi. Permintaan pasar ekspor pada negara Asia, Eropa, dan Amerika maupun lokal cukup tinggi dengan nilai jual yang mahal. Akan tetapi, terdapat kendala yang sering terjadi pada budidaya pembesaran lobster yaitu infeksi penyakit yang akan menyebabkan tingginya mortalitas Tujuan penelitian ini adalah untuk menginvestigasi penyakit infeksi pada pembesaran lobster Panulirus spp. yang dipelihara di keramba jaring apung Tanjung Putus, Lampung. Metode penelitian ini meliputi pengambilan sampel lobster sebanyak lima ekor dalam karamba jarring apung (KJA), pengamatan gejala klinis, isolasi bakteri, identifikasi bakteri, deteksi Milky Hemolymph Disease of Spiny Lobster (MHD-SL) melalui analisis Polymerase Chain Reaction (PCR), dan histopatologi. Lokasi pengambilan sampel di PT. Saibatin Perikanan Indonesia, Lampung. Terdapat bakteri Vibrio parahaemolyticus dan V. alginolyticus. Lima ekor lobster terinfeksi oleh MHD-SL yang ditandai dengan hemolim berwarna putih susu. Lima sampel lobster positif terinfeksi penyakit Milky Hemolymph Disease of Spiny Lobster (MHD-SL) melalui analisis PCR. Hasil pengamatan histopatologi terdapat perubahan mikroanatomi patologi pada jaringan hepatopankreas lobster menunjukkan bahwa adanya perubahan jaringan berupa
刺龙虾属于甲壳类动物,具有很高的经济价值。亚洲、欧洲和美洲国家以及本地出口市场的需求量相当大,售价昂贵。然而,刺龙虾饲养养殖过程中经常会遇到一些障碍,即传染病会导致高死亡率。本研究的目的是调查在楠榜丹绒普陀浮动网箱中饲养的刺龙虾(Panulirus spp.)的传染病。研究方法包括在浮动网笼中抽取五只棘龙虾样本、观察临床症状、分离细菌、鉴定细菌、通过聚合酶链式反应(PCR)分析和组织病理学检测棘龙虾乳状血淋巴病(MHD-SL)。取样地点位于印度尼西亚PT.Saibatin Perikanan Indonesia。检测到副溶血性弧菌和溶藻弧菌。五只龙虾感染了MHD-SL,血淋巴呈乳白色。通过聚合酶链式反应(PCR)分析,所有五个龙虾样本均被确诊感染了棘龙虾乳白色血淋巴病(MHD-SL)。组织病理学观察显示,龙虾肝胰腺组织发生了病理微观解剖学变化,表明这些组织变化以包裹和浸润的形式出现,是由于类立克次体细菌(RLB)感染所致。为了预防浮游网箱中的刺龙虾MHD-SL病,必须对刺龙虾的放养密度进行管理,维护生物安全,并提高龙虾的免疫力。 关键词:细菌 组织病理学 MHD-SL Panulirus spp. ABSTRAK Lobster merupakan anggota crustacea yang memiliki nilai ekonomis yang tinggi.龙虾在亚洲、欧洲和美洲及内陆地区的养殖量一直居高不下。在楠榜丹绒普陀山的龙虾饲养场,正在对龙虾的死亡原因进行调查。该研究的方法包括龙虾的病原体、临床研究、龙虾分离、龙虾鉴定、通过聚合酶链式反应(PCR)分析检测棘龙虾的乳状淋巴结病(MHD-SL)以及组织病理学。Lokasi pengambilan sampel di PT.Saibatin Perikanan Indonesia, Lampung.Terdapat bakteri Vibrio parahaemolyticus dan V. alginolyticus.MHD-SL 提供的利马贻贝龙虾的血液中含有大量的致病菌。通过聚合酶链反应(PCR)分析,龙虾样品中的阳性结果为棘龙虾乳状淋巴结病(MHD-SL)。组织病理学研究表明,龙虾的肝脏中存在大量的立克次体样细菌(RLB),这表明龙虾的肝脏中存在大量的立克次体样细菌(RLB)。龙虾的饲养管理、龙虾的生物安全以及龙虾的免疫系统都是为了在 KJA 减少 MHD-SL 的感染。 Kata kunci: bakteri, histopatologi, MHD-SL, Panulirus spp.
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引用次数: 0
Inulin Inulin supplementation in feed as a prebiotic for red tilapia Oreochromis sp. 在饲料中添加菊粉菊粉作为红罗非鱼的益生元
Pub Date : 2024-01-12 DOI: 10.19027/jai.23.1.36-43
Agustina Agustina, Adi Susanto
The objective of this study was to assess the impact of inulin as a prebiotic in feed on red tilapia (Oreochromis sp.) growth performance, hematological parameters, feed utilization, digestive enzyme activity, and the quantity of lactic acid bacteria in the intestine. This study has a fully randomized design with four treatments and three replications, namely inulin at doses of 0, 1, 2, and 4 g/kg feed. One-way ANOVA was used to assess data on growth performance, feed utilization, survival rate, digestive enzyme activity, and the amount of lactic acid bacteria, while the hematological parameters were analyzed descriptively. Red tilapia fry weighing an average of 5.27 ± 0.45 g was raised for 30 days in plastic container containing 40 L of water at a density of 15 fish per container (375 fish/m3). Fish were fed three times a day at satiation. Red tilapia-fed diet supplemented with 4 g/kg inulin of feed had higher body weight increase, protein efficiency ratio, digestive enzyme activity, and amount of lactic acid bacteria while food conversion ratio was lower compared to other treatments (p<0.05). Meanwhile, the specific growth rate and survival rate were not different from the control group. Hematological parameters were within the normal range for all treatments. Thus, the supplementation of inulin in feed of 4 g/kg has the potential as a prebiotic for red tilapia.   Keywords: digestive enzyme activity, inulin, lactic acid bacteria, prebiotic, red tilapia   ABSTRAK   Tujuan dari penelitian ini adalah untuk mengkaji pengaruh inulin sebagai prebiotik pada pakan terhadap kinerja pertumbuhan, parameter hematologi, pemanfaatan pakan, aktivitas enzim pencernaan, dan kuantitas bakteri asam laktat di usus ikan nila merah (Oreochromis sp.). Penelitian ini memiliki rancangan acak lengkap dengan empat perlakuan dan tiga ulangan yaitu inulin dengan dosis 0; 1; 2; dan 4 g inulin/kg pakan. One-way ANOVA digunakan untuk menilai data kinerja pertumbuhan, pemanfaatan pakan, kelangsungan hidup, aktivitas enzim pencernaan, dan jumlah bakteri asam laktat, sedangkan parameter hematologi dianalisis secara deskriptif. Benih ikan nila merah dengan berat rata-rata 5,27 ± 0,45 g dipelihara selama 30 hari dalam wadah plastik dengan volume air 40 L dan kepadatan 15 ekor per wadah (375 ekor/m3). Ikan diberi makan tiga kali sehari secara at satiation. Ikan nila merah yang diberi suplemen inulin 4 g/kg pakan menunjukkan pertumbuhan bobot, rasio efisiensi protein, aktivitas enzim pencernaan, dan jumlah bakteri asam laktat yang lebih besar, sedangkan konversi pakan lebih rendah dibandingkan dengan perlakuan lainnya (p<0,05). Parameter laju pertumbuhan spesifik dan tingkat kelangsungan hidup tidak berbeda dengan kelompok kontrol Parameter hematologi berada dalam kisaran normal untuk semua perlakuan. Suplementasi inulin pada pakan sebanyak 4 g/kg berpotensi sebagai prebiotik ikan nila merah.   Kata kunci: aktivitas enzim pencernaan, bakteri asam laktat, ikan nila merah, inu
本研究旨在评估饲料中添加菊粉作为益生元对红罗非鱼(Oreochromis sp.)生长性能、血液学参数、饲料利用率、消化酶活性和肠道乳酸菌数量的影响。本研究采用完全随机设计,四个处理三个重复,即菊粉剂量为 0、1、2 和 4 克/千克饲料。采用单因素方差分析评估了生长性能、饲料利用率、存活率、消化酶活性和乳酸菌数量等数据,并对血液学参数进行了描述性分析。红罗非鱼苗平均体重为 5.27 ± 0.45 克,在装有 40 升水的塑料容器中养殖 30 天,密度为每个容器 15 条鱼(375 条/立方米)。每天喂食三次,直至鱼吃饱。与其他处理相比,红罗非鱼饲喂添加 4 g/kg 菊粉的饲料,体重增加率、蛋白质效率比、消化酶活性和乳酸菌数量都较高,而食物转化率较低(p<0.05)。同时,特定生长率和存活率与对照组无差异。所有处理的血液指标均在正常范围内。因此,在饲料中添加 4 克/千克的菊粉可作为红罗非鱼的益生元。 关键词消化酶活性 菊粉 乳酸菌 益生素 红色罗非鱼 ABSTRAK Tujuan dari penelitian ini adalah untuk mengkaji pengaruh inulin sebagai prebiotik pada pakan terhadap kinerja pertumbuhan、这些营养成分包括血液学参数、蛋白质含量、胰蛋白酶活性和鲑鱼(Oreochromis sp.).该研究表明,菊粉的剂量为 0、1、2 和 4 克菊粉/千克,可提高鲤鱼的生长速度,增加鲤鱼的饲养量。通过单因素方差分析,可以得出胰蛋白酶、胰蛋白激酶、胰蛋白酶活性、胰蛋白酶胰岛素和胰蛋白酶胰岛素含量等数据,同时还可以得出血液学参数。在 30 天内,该动物的体重为 5.27 ± 0.45 克,而塑料容器的容积为 40 升,每容器的重量为 15 克(375 克/立方米)。从现在开始到饱和为止,每小时可使用一次。从每公斤 4 克菊粉中摄入的菊粉能提高面包的饱腹感、蛋白质的效率、胰蛋白酶的活性和面包的口感,而从每公斤中摄入的菊粉能降低面包的口感(P<0.05)。血液学参数与对照组的血液学参数相比,正常值与每千克血浆中的正常值相差甚远。每公斤 4 克的菊粉可作为益生菌补充剂。 关键字:酵母、烘焙、婴幼儿、菊粉、益生元
{"title":"Inulin Inulin supplementation in feed as a prebiotic for red tilapia Oreochromis sp.","authors":"Agustina Agustina, Adi Susanto","doi":"10.19027/jai.23.1.36-43","DOIUrl":"https://doi.org/10.19027/jai.23.1.36-43","url":null,"abstract":"The objective of this study was to assess the impact of inulin as a prebiotic in feed on red tilapia (Oreochromis sp.) growth performance, hematological parameters, feed utilization, digestive enzyme activity, and the quantity of lactic acid bacteria in the intestine. This study has a fully randomized design with four treatments and three replications, namely inulin at doses of 0, 1, 2, and 4 g/kg feed. One-way ANOVA was used to assess data on growth performance, feed utilization, survival rate, digestive enzyme activity, and the amount of lactic acid bacteria, while the hematological parameters were analyzed descriptively. Red tilapia fry weighing an average of 5.27 ± 0.45 g was raised for 30 days in plastic container containing 40 L of water at a density of 15 fish per container (375 fish/m3). Fish were fed three times a day at satiation. Red tilapia-fed diet supplemented with 4 g/kg inulin of feed had higher body weight increase, protein efficiency ratio, digestive enzyme activity, and amount of lactic acid bacteria while food conversion ratio was lower compared to other treatments (p<0.05). Meanwhile, the specific growth rate and survival rate were not different from the control group. Hematological parameters were within the normal range for all treatments. Thus, the supplementation of inulin in feed of 4 g/kg has the potential as a prebiotic for red tilapia. \u0000  \u0000Keywords: digestive enzyme activity, inulin, lactic acid bacteria, prebiotic, red tilapia \u0000  \u0000ABSTRAK \u0000  \u0000Tujuan dari penelitian ini adalah untuk mengkaji pengaruh inulin sebagai prebiotik pada pakan terhadap kinerja pertumbuhan, parameter hematologi, pemanfaatan pakan, aktivitas enzim pencernaan, dan kuantitas bakteri asam laktat di usus ikan nila merah (Oreochromis sp.). Penelitian ini memiliki rancangan acak lengkap dengan empat perlakuan dan tiga ulangan yaitu inulin dengan dosis 0; 1; 2; dan 4 g inulin/kg pakan. One-way ANOVA digunakan untuk menilai data kinerja pertumbuhan, pemanfaatan pakan, kelangsungan hidup, aktivitas enzim pencernaan, dan jumlah bakteri asam laktat, sedangkan parameter hematologi dianalisis secara deskriptif. Benih ikan nila merah dengan berat rata-rata 5,27 ± 0,45 g dipelihara selama 30 hari dalam wadah plastik dengan volume air 40 L dan kepadatan 15 ekor per wadah (375 ekor/m3). Ikan diberi makan tiga kali sehari secara at satiation. Ikan nila merah yang diberi suplemen inulin 4 g/kg pakan menunjukkan pertumbuhan bobot, rasio efisiensi protein, aktivitas enzim pencernaan, dan jumlah bakteri asam laktat yang lebih besar, sedangkan konversi pakan lebih rendah dibandingkan dengan perlakuan lainnya (p<0,05). Parameter laju pertumbuhan spesifik dan tingkat kelangsungan hidup tidak berbeda dengan kelompok kontrol Parameter hematologi berada dalam kisaran normal untuk semua perlakuan. Suplementasi inulin pada pakan sebanyak 4 g/kg berpotensi sebagai prebiotik ikan nila merah. \u0000  \u0000Kata kunci: aktivitas enzim pencernaan, bakteri asam laktat, ikan nila merah, inu","PeriodicalId":32090,"journal":{"name":"Jurnal Akuakultur Indonesia","volume":" 7","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139624161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Identification of Kappaphycus alvarezii seaweed based on phylogenetic and carrageenan conten 根据系统发育和卡拉胶含量鉴定 Kappaphycus alvarezii 海藻
Pub Date : 2024-01-10 DOI: 10.19027/jai.23.1.1-11
G. I. Satriani, D. Soelistyowati, A. Alimuddin, H. Arfah, Irzal Effendi
Increasing seaweed production requires accurate information regarding the genetic sources of seeds used. Identifying the seaweed species Kappaphycus molecular is one of the solutions to ensure seaweed cultivators choose seeds for their cultivation businesses. Molecular identification is essential for the system traceability of seaweed products and the creation of databases regarding species variant information Kappaphycus alvarezii cultivation as potential data collection for developing and genetically breeding seaweed seeds. To date, there is no information on the genetic potential of K. alvarezii cultivated in various seaweed cultivation centers in Indonesia. This study aimed to obtain phylogenetic details based on identification of the genetic source using DNA molecular markers barcoding rbcL and analysis of carrageenan content using the Fourier transform infra-red (FTIR) spectrum. The results of DNA sequencing analysis and FTIR testing of 16 varieties of seaweed seedlings obtained from various cultivation centers in Indonesia showed 99% similarity with K. alvarezii, a producer of kappa carrageenan.   Keywords: DNA sequencing, phylogenetics, haplotypes, kappa-carrageenan, rbcL     ABSTRAK   Peningkatan produksi rumput laut memerlukan informasi yang akurat mengenai kepastian sumber genetik bibit yang digunakan. Identifikasi spesies rumput laut Kappaphycus secara molekuler merupakan salah satu solusi untuk memberikan kepastian pada pembudidaya rumput laut untuk memilih bibit bagi usaha budidaya. Identifikasi molekuler sangat penting dalam sistem traceability produk rumput laut dan pembuatan basis data mengenai informasi varian spesies Kappaphycus alvarezii budidaya sebagai pendataan potensi untuk pengembangan dan pemuliaan bibit rumput laut secara genetis. Sampai saat ini belum tersedia informasi mengenai potensi genetik rumput laut K. alvarezii yang dibudidayakan di berbagai sentra budidaya rumput laut di Indonesia. Penelitian ini bertujuan untuk memperoleh informasi filogenetik berdasarkan identifikasi sumber genetiknya menggunakan penanda molekuler DNA barcoding rbcL serta analisis kandungan karaginannya menggunakan spektrum Fourier Transform Infra-Red (FTIR). Dari hasil analisis sekuensing DNA dan pengujian FTIR terhadap 16 varietas bibit rumput laut yang diperoleh di berbagai sentra budidaya di Indonesia menghasilkan 99% kemiripan yang tinggi dengan K. alvarezii penghasil kappa karagenan.   Kata kunci: DNA sekuensing, filogenetik, haplotipe, kappa-karagenan, rbcL
要提高海藻产量,就必须准确了解所用种子的基因来源。分子鉴定海藻品种 Kappaphycus 是确保海藻种植者为其种植业务选择种子的解决方案之一。分子鉴定对于海藻产品的系统可追溯性和创建有关 Kappaphycus alvarezii 栽培物种变异信息的数据库至关重要,可作为开发和遗传育种海藻种子的潜在数据收集。迄今为止,还没有关于印度尼西亚各海藻培育中心培育的 K. alvarezii 遗传潜力的信息。本研究旨在利用 DNA 分子标记条形码 rbcL 鉴定基因来源,并利用傅立叶变换红外光谱分析卡拉胶含量,从而获得系统发育的详细信息。对从印度尼西亚不同栽培中心获得的16个品种的海藻苗进行DNA测序分析和傅立叶变换红外光谱检测的结果表明,它们与K. alvarezii的相似度为99%,K. alvarezii是kappa卡拉胶的生产者。 关键词:K:DNA 测序 系统发育 单倍型 kappa 角叉菜胶 rbcL ABSTRACT Increasing seaweed production requires accurate information on the genetic source of the seedlings used.Kappaphycus 海藻物种的分子鉴定是为海藻养殖者提供确定性以选择栽培种子的一种解决方案。分子鉴定对于海藻产品的可追溯系统和建立有关栽培卡帕藻变种信息的数据库非常重要,可为海藻种子的遗传开发和育种收集潜在数据。到目前为止,还没有关于印度尼西亚各海藻培育中心培育的 K. alvarezii 海藻遗传潜力的信息。本研究旨在利用分子标记 DNA 条形码 rbcL 鉴定基因来源,并利用傅立叶变换红外光谱(FTIR)分析卡拉胶含量,从而获得系统发育信息。对印度尼西亚不同培育中心获得的 16 种海藻苗进行 DNA 测序分析和傅立叶变换红外光谱检测的结果表明,这些海藻苗与生产卡帕卡拉胶的 K. alvarezii 的相似度高达 99%。 关键词DNA 测序 系统发育 单倍型 卡帕卡拉胶 rbcL
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引用次数: 0
The influence of biofloc system on Vibrio composition, the growth and the gut microvilli performance of the Pacific white shrimp Penaeus vannamei 生物絮凝物系统对太平洋南美白对虾弧菌组成、生长和肠道微绒毛性能的影响
Pub Date : 2024-01-10 DOI: 10.19027/jai.23.1.12-23
Muhamad Gustilatov, W. Widanarni, J. Ekasari, Pande Gde Sasmita Julyantoro, D. Waturangi
Biofloc technology has been shown to have a positive impact on shrimp culture by controlling pathogenic Vibrio. This study aimed to analyze the effect of biofloc on the Vibrio composition in water and shrimp gut, as well as the growth performance and microvilli of Pacific white shrimp (Penaeus vannamei). Shrimp post-larvae measuring 0.66 ± 0.02 g were reared in a glass aquarium (working volume 22 L) of 6 units with a density of 150 shrimp m-3 for 28 days. The treatments included rearing in the biofloc system with C/N ratio 10, and without a biofloc system as a control. The results showed that Vibrio was highly prevalent in the control gut (4.13%) and biofloc water (3.77%), but only a few were found in the control water (0.16%) and biofloc-treated gut (0.11%). V. hepatarius (1.10%) and V. nereis (1.06%) were found to dominate the Vibrio bacterial community in the biofloc system maintenance media, while Vibrio sp. Hep-1b-8 (2.26%) and V. parahaemolyticus (0.80%) known as a pathogenic bacteria dominated the control shrimp gut. The biofloc system significantly increased the digestive enzyme activity, growth performance, and microvilli length in the shrimp gut. In conclusion, the application of a biofloc system in shrimp culture can affect the composition and abundance of bacterial communities in both the culture environment and the shrimp gut, and improve growth performance with higher digestive enzyme activity and longer microvilli in the gut.   Keywords: biocontrol, biofloc, microbiota, shrimp, Vibrio   ABSTRAK   Teknologi bioflok menunjukkan dampak positif pada kegiatan budidaya udang vaname dengan mengendalikan bakteri patogen salah satunya Vibrio. Penelitian ini bertujuan untuk menganalisis efek bioflok terhadap komposisi Vibrio pada  air dan usus udang, serta kinerja pertumbuhan dan mikrovili udang vaname (Penaeus vanamei). Udang post-larva berukuran 0.66 ± 0.02 g dipelihara pada akuarium kaca (volume air 22 L) sebanyak 6 unit dengan kepadatan 150 ekor m-3 selama 28 hari. Perlakuan meliputi pemeliharaan udang pada sistem bioflok dengan rasio C/N 10, dan tanpa sistem bioflok sebagai perlakuan kontrol. Hasil pengamatan menunjukkan kelimpahan Vibrio yang cukup tinggi pada usus perlakuan kontrol (4.13%) dan air perlakuan bioflok (3.77%), tetapi sangat sedikit ditemukan pada air perlakuan kontrol dan usus perlakuan bioflok. Selanjutnya, V. hepatarius (2.26%) dan V. nereis (1.06%) terdeteksi mendominasi komunitas bakteri Vibrio di media pemeliharaan pada sistem bioflok, sedangkan Vibrio sp. Hep-1b-8 (2.26%) dan V. parahaemolyticus (0.80%) mendominasi usus udang vaname pada perlakuan kontrol. Sistem bioflok juga mampu meningkatkan aktivitas enzim pencernaan, performa pertumbuhan, dan panjang mikrovili usus secara signifikan (P < 0.05). Kesimpulan dari penelitian ini adalah penerapan sistem bioflok pada budidaya udang vaname mampu memengaruhi komposisi dan kelimpahan komunitas bakteri Vibrio pada lingkungan budidaya maupun pada usus udang, serta
生物絮团技术通过控制致病弧菌对对虾养殖产生了积极影响。本研究旨在分析生物絮凝物对水体和对虾肠道中弧菌成分的影响,以及对太平洋白对虾(Penaeus vannamei)生长性能和微绒毛的影响。在 6 个单位的玻璃水族箱(工作容积 22 升)中饲养对虾后幼体(0.66 ± 0.02 克),密度为 150 虾 m-3,饲养 28 天。处理包括在 C/N 比为 10 的生物絮凝系统中饲养和不使用生物絮凝系统作为对照。结果表明,弧菌在对照组肠道(4.13%)和生物絮凝物水(3.77%)中的流行率很高,但在对照组水(0.16%)和生物絮凝物处理过的肠道(0.11%)中只发现了少数弧菌。在生物絮凝系统维护介质中,肝弧菌(1.10%)和奈雷弧菌(1.06%)在弧菌群落中占主导地位,而在对照组对虾肠道中,Hep-1b-8弧菌(2.26%)和副溶血性弧菌(0.80%)(被称为致病菌)占主导地位。生物絮凝物系统大大提高了对虾肠道中消化酶的活性、生长性能和微绒毛长度。总之,在对虾养殖中应用生物絮凝物系统可以影响养殖环境和对虾肠道中细菌群落的组成和丰度,并通过提高消化酶活性和延长肠道微绒毛来改善生长性能。 关键词:生物控制;生物絮团;微生物群;对虾;弧菌 ABSTRAK Teknologi bioflok menunjukkan dampak positif pada kegiatan budidaya udang vaname dengan mengendalikan bakteri patogen salah satunya Vibrio.该研究的目的是提高空气和鱼类中弧菌的生物流动性,以及万年青藻(Penaeus vanamei)的生长和繁殖能力。28 天后,在 6 个单位的水槽(空气容积 22 升)中,在 150 ekor m-3 的温度下,饵料中的饵料含量为 0.66 ± 0.02 克。生物流体系统和生物流体对照系统分别在 C/N 10 和 C/N 10 范围内对乌塘的生长进行了监测。结果表明,对照组空气中的弧菌(4.13%)和生物流体中的空气中的弧菌(3.77%)都很高,而对照组空气中的弧菌和生物流体中的弧菌都很低。在生物流系统中,肝弧菌(2.26%)和尼氏弧菌(1.06%)在培养基中形成了弧菌群体,而 Hep-1b-8 型弧菌(2.26%)和副溶血性弧菌(0.80%)在对照组中形成了弧菌群体。生物流系统也能显著提高肠道蠕动能力、肠道蠕动性能和肠道蠕动潜能(P < 0.05)。从笔者的研究结果来看,在乌当芽孢杆菌中使用生物流体系统可提高芽孢杆菌和乌当弧菌的组成和繁殖能力,同时提高弧菌的繁殖能力和弧菌的传播能力。 Kata kunci: 生物流、生物控制、微生物区系、香草、弧菌
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引用次数: 0
Nursery of red tilapia Oreochromis niloticus in a small-scale aquaponics system with different stocking densities 在不同放养密度的小型鱼菜共生系统中培育红罗非鱼
Pub Date : 2023-11-24 DOI: 10.19027/jai.22.2.210-219
Y. Hadiroseyani, Anita May Sharly, Dadang Shafruddin, A. Vinasyiam
Aquaponics is a fish and hydroponic plant-rearing system that utilises fish waste as a plant nutrient. Because water quality can be maintained at an optimal level for fish in the system to enhance farming productivity. This study was conducted to evaluate the effects of fish density on the nursery stage of red tilapia in a small-scale aquaponic system. The study was conducted using a completely randomized design at three levels of stocking density, namely 250 fish/m2, 375 fish/m2, and 500 fish/m2 of red tilapia at an initial size of 6.8 ± 0.35 cm, with 30 net pots of bok choy plant as co-culture. The results that the increase in fish population escalated the productivity of both fish and vegetable in 30 days farming period. The stocking density did not affect fish survival 99 ± 0.82%, 97 ± 1.27%, and 97 ± 0.68%, respectively (p>0.05), but supported a better specific growth rate, namely 3.09 ± 0.30%/day; 3.57 ± 0.23%/day; 4.03 ± 0.04%/day (p<0.05). Interestingly, the smallest coefficient of diversity and lowest feed conversion ratio were obtained at 500 fish/m3, whereas absolute length growth did not differ. Bok choy production increased with fish density, namely 2.82 ± 0.06 kg; 2.88 ± 0.08 kg; 3.17 ± 0.10 kg, respectively (p<0.05). The water quality parameter values were almost identical in all treatments, except for the lowest bacterial abundance gained at stocking density of 500 fish/m3. In conclusion, the aquaponic system can be used to nurseries tilapia seeds at high stocking densities for production efficiency.   Keywords: aquaculture, bok choy, hydroponics, intensification   ABSTRAK   Akuaponik adalah sistem pemeliharaan ikan dan tanaman hidroponik yang memanfaatkan kotoran ikan sebagai nutrisi tanaman. Karena kualitas air dapat dipertahankan pada tingkat optimal untuk ikan dalam sistem akuaponik untuk meningkatkan produktivitas budidaya. Penelitian ini dilakukan mengevaluasi pengaruh peningkatan kepadatan ikan pada tahap pendederan ikan nila merah dalam sistem akuaponik skala kecil. Penelitian dilakukan dengan menggunakan rancangan acak lengkap pada tiga tingkat kepadatan tebar yaitu 250 ekor/m2, 375 ikan/m2, dan ikan nila merah sebanyak 500 ekor/m2 dengan ukuran awal 6,8 ± 0,35 cm, pada masing-masing sistem aquaponik dengan 30 net pot tanaman bok choy. Hasil penelitian menunjukkan peningkatan populasi ikan telah meningkatkan produktivitas ikan dan sayuran dalam masa budidaya 30 hari. Peningkatan padat penebaran tidak berpengaruh terhadap kelangsungan hidup ikan yaitu berturut-turut sebesar 99 ± 0,82%, 97 ± 1,27%, dan 97 ± 0,68% (p>0,05), namun mampu mendukung laju pertumbuhan spesifik yang lebih baik yaitu 3,09 ± 0,30%/hari; 3,57 ± 0,23%/hari; dan 4,03 ± 0,04%/hari (p<0,05). Menariknya, koefisien keanekaragaman terkecil dan rasio konversi pakan terendah diperoleh pada kepadatan 500 ekor/m3, sedangkan pertumbuhan panjang absolut tidak berbeda. Produksi bok choy meningkat seiring dengan kepadatan ikan yaitu berturut-turut 2,82 ± 0,06
鱼菜共生是一种利用鱼类排泄物作为植物养分的鱼类和水耕植物饲养系统。因为在该系统中,水质可以保持在鱼类的最佳水平,从而提高养殖产量。本研究旨在评估鱼类密度对小型水生植物栽培系统中红色罗非鱼育苗阶段的影响。研究采用完全随机设计法,在初始规格为 6.8 ± 0.35 厘米的红罗非鱼的三个放养密度水平,即 250 条鱼/平方米、375 条鱼/平方米和 500 条鱼/平方米,与 30 个网盆的大白菜共同养殖。结果表明,在 30 天的养殖期内,鱼类数量的增加提高了鱼类和蔬菜的产量。放养密度并不影响鱼的存活率,分别为 99 ± 0.82%、97 ± 1.27%和 97 ± 0.68%(p>0.05),但支持较好的特定生长率,即 3.09 ± 0.30%/天;3.57 ± 0.23%/天;4.03 ± 0.04%/天(p0.05),但支持较好的特定增长率,即 3.09 ± 0.30%/天;3.57 ± 0.23%/天;4.03 ± 0.04%/天(p<0.05)。有趣的是,鱼的密度为 500 尾/立方米时,多样性系数最小,饲料转化率最低,而绝对长度的增长没有差异。大白菜产量随着鱼密度的增加而增加,分别为 2.82 ± 0.06 kg、2.88 ± 0.08 kg 和 3.17 ± 0.10 kg(p<0.05)。除放养密度为 500 尾/立方米时细菌丰度最低外,所有处理的水质参数值几乎相同。总体而言,鱼类和植物产量在放养密度为 500 尾/立方米时最高。总之,水培系统可用于罗非鱼育苗,高密度放养可提高生产效率。 关键词:水产养殖、水培、集约化、白鲑鱼
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Jurnal Akuakultur Indonesia
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