Pub Date : 2022-10-25DOI: 10.19027/jai.21.2.178-185
D. Liubana, Joi Alfreddi Surbakt, Claudya Zyta Tobu
ABSTRACT Sand sea cucumber (Holothuria scabra) is a high economic value as a food source. Sea cucumbers contain beneficial bioactive compounds for health. This study determined the sea cucumber growth cultured in an integrated multi-trophic aquaculture (IMTA) with different water exchange systems in Lifuleo waters from October to December, 2020. This study was performed in an experimental method with three different exchange system treatments and three replications, namely once every 2 days (tank A), every day (tank B), and water circulation every day (tank C). Briefly, sand sea cucumbers with 116.72 ± 117.91 g body weight and 11.06 ± 11.83 cm length were maintained for 45 days with different water exchange systems and fed with Eucheuma cottonii. The results showed that the best water exchange systems to increase sand sea cucumber production was tank A with 100% survival rate, 1.84 ± 0.06%/day specific growth rate, and 1714.96 ± 34.13 μm length growth rate. The water quality parameters were also optimal during the sand sea cucumber with the integrated system. Keywords: Multi-trophic, growth, circulation, integrated system, sand sea cucumber ABSTRAK Teripang pasir (Holothuria scabra) mempunyai nilai ekonomis tinggi dan diperdagangkan sebagai bahan pangan. Selain itu teripang juga mengandung senyawa bioaktif yang bermanfaat untuk kesehatan. Penelitian ini mengkaji tentang pertumbuhan teripang yang dibudidayakan secara integrated multi-trophic aquaculture (IMTA) dengan sistem pergantian air yang berbeda di perairan Lifuleo pada bulan Oktober sampai Desember 2020. Penelitian ini dilakukan dengan metode eksperimental dengan tiga perlakuan 3 ulangan dengan sistem pergantian air 2 hari sekali (bak A), pergantian air setiap hari (bak B) dan sirkulasi air dilakukan setiap hari (bak C). Secara singkat, teripang dengan bobot badan 116.72 ± 117.91 gr dan panjang 11.06 ± 11.83 cm dipelihara selama 45 hari dengan sistem pergantian air dan diberi makan dengan Eucheuma cottonii. Hasil penelitian menunjukkan bahwa media perlakuan teripang dengan sistem pergantian air yang berbeda terbaik untuk meningkatkan produksi teripang adalah bak A dengan tingkat kelangsungan hidup 100, laju pertumbuhan spesifik harian 1.84 ± 0.06%/hari dan laju pertumbuhan panjang 1714.96 ± 34.13 µm. Parameter kualitas air juga menunjukkan kualitas yang optimal pada budidaya teripang secara terintegrasi. Kata kunci: Multitropic, pertumbuhan, sirkulasi, terintegrasi, teripang pasir
沙海参(Holothuria scabra)具有很高的经济价值。海参含有有益健康的生物活性化合物。本研究于2020年10 - 12月对利富里奥海域不同换水系统的综合多营养养殖(IMTA)海参生长进行了研究。试验采用3种不同换水系统处理，3个重复，即每2 d 1次(A池)、每天1次(B池)、每天1次(C池)。以体重为116.72±117.91 g、体长为11.06±11.83 cm的沙海参为研究对象，在不同换水系统下饲养45 d，以棉真菊为饲料。结果表明，提高沙海参产量的最佳换水系统为A池，其成活率为100%，比生长率为1.84±0.06%/d，长度为1714.96±34.13 μm。采用该综合系统对沙海参养殖过程中的水质参数进行了优化。关键词:多营养、生长、循环、综合系统、沙海参Selain itu teripang juga mengandung senyawa bioakf yang manfak untuk keshaan。Penelitian ini mengkaji tenang pertumbuhan teripang dibudidayakan secara综合多营养水产养殖(IMTA)登干系统pertianan air yang berbeda di perairan Lifuleo padbulan 2020年10月3日12月Penelitian ini dilakukan dengan方法实验dengan tiga perlakan 3 ulangan dengan system perengan air 2 hari sekali (bak A)， perganan dengan hari (bak B) dan sirkulasi air dilakukan setiap hari (bak C). Secara singkat, teripang dengan bobot badan 116.72±117.91 gr dan panjang 11.06±11.83 cm dipelihara selama 45 hari dengan system perengan air dan diberi makan dengan Eucheuma cottonii。Hasil penelitian menunjukkan bahwa media perlakuan teripang dengan system perperantian air yang berbeda terbaik untuk meningkatkan produksi teripang adalah bak A dengan tingkat kelangsungan hidup 100, laju pertumbuhan spifik harian 1.84±0.06%/hari dan laju pertumbuhan panjang 1714.96±34.13µm。参数kualitas air juga menunjukkan kualitas yang最优pada buddaya teripang secara terintegrasi。Kata kunci:多品种，pertumbuhan, sirkulasi, terintegrasi, teripang pasir
{"title":"Cultured sand sea cucumber growth with different water exchange systems","authors":"D. Liubana, Joi Alfreddi Surbakt, Claudya Zyta Tobu","doi":"10.19027/jai.21.2.178-185","DOIUrl":"https://doi.org/10.19027/jai.21.2.178-185","url":null,"abstract":"ABSTRACT \u0000Sand sea cucumber (Holothuria scabra) is a high economic value as a food source. Sea cucumbers contain beneficial bioactive compounds for health. This study determined the sea cucumber growth cultured in an integrated multi-trophic aquaculture (IMTA) with different water exchange systems in Lifuleo waters from October to December, 2020. This study was performed in an experimental method with three different exchange system treatments and three replications, namely once every 2 days (tank A), every day (tank B), and water circulation every day (tank C). Briefly, sand sea cucumbers with 116.72 ± 117.91 g body weight and 11.06 ± 11.83 cm length were maintained for 45 days with different water exchange systems and fed with Eucheuma cottonii. The results showed that the best water exchange systems to increase sand sea cucumber production was tank A with 100% survival rate, 1.84 ± 0.06%/day specific growth rate, and 1714.96 ± 34.13 μm length growth rate. The water quality parameters were also optimal during the sand sea cucumber with the integrated system. \u0000Keywords: Multi-trophic, growth, circulation, integrated system, sand sea cucumber \u0000 \u0000ABSTRAK \u0000Teripang pasir (Holothuria scabra) mempunyai nilai ekonomis tinggi dan diperdagangkan sebagai bahan pangan. Selain itu teripang juga mengandung senyawa bioaktif yang bermanfaat untuk kesehatan. Penelitian ini mengkaji tentang pertumbuhan teripang yang dibudidayakan secara integrated multi-trophic aquaculture (IMTA) dengan sistem pergantian air yang berbeda di perairan Lifuleo pada bulan Oktober sampai Desember 2020. Penelitian ini dilakukan dengan metode eksperimental dengan tiga perlakuan 3 ulangan dengan sistem pergantian air 2 hari sekali (bak A), pergantian air setiap hari (bak B) dan sirkulasi air dilakukan setiap hari (bak C). Secara singkat, teripang dengan bobot badan 116.72 ± 117.91 gr dan panjang 11.06 ± 11.83 cm dipelihara selama 45 hari dengan sistem pergantian air dan diberi makan dengan Eucheuma cottonii. Hasil penelitian menunjukkan bahwa media perlakuan teripang dengan sistem pergantian air yang berbeda terbaik untuk meningkatkan produksi teripang adalah bak A dengan tingkat kelangsungan hidup 100, laju pertumbuhan spesifik harian 1.84 ± 0.06%/hari dan laju pertumbuhan panjang 1714.96 ± 34.13 µm. Parameter kualitas air juga menunjukkan kualitas yang optimal pada budidaya teripang secara terintegrasi. \u0000Kata kunci: Multitropic, pertumbuhan, sirkulasi, terintegrasi, teripang pasir","PeriodicalId":32090,"journal":{"name":"Jurnal Akuakultur Indonesia","volume":"23 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80847066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ABSTRACT This study aims to determine dose of black cumin flour in fish feed diets which can improve the disease resistance of tilapia against the attack of A. hydrophila. This study was used tilapia juvenile with a size of 7 – 8 cm. This study was conducted from March – May 2021 and the experimental method is completely randomized design (CRD) with 5 treatments and 3 replications. The treatment used were addition of black cumin flour into fish feed with doses 0 (A), 20 (B), 35 (C), 50 (D), and 65 (E) g/Kg feed. The treatments fed by supplemental diets contained black cumin flour was conducted for 14 days, then a challenge test was carried out with the infection of A. hydrophila bacteria by immersion for14 days. A. hydrophila bacteria used to infect fish had a density of 108 CFU/mL. The Parameters used were total leukocyte, hematocrit, total leukocyte differential, clinical symptoms, survival rate and water quality. The result showed that black cumin flour was effective to increase the immunity system of tilapia. The dose of 50 g/Kg feed was effective showed increase the highest percentage increase in white blood cells of 64.56%, compared to treatments B, C, and E which showed an increase in white blood cells of 47.40%, 48.82% and 1.2%, respectively. Treatment A decreased the percentage of white blood cells by 19.52%. The 50 g dose of black cumin showed the highest percentage value of hematocrit and leukocyte differential in tilapia fry, respectively 34%, lymphocytes 84.3%, monocytes 6%, and neutrophils 9.7% with the fastest recovery period (4 days) and after the challenge test resulted in the highest survival rate of 95%. Keywords: Aeromonas hydrophila, tilapia juvenile, black cumin, immune system ABSTRAK Penelitian ini bertujuan untuk menentukan dosis tepung jintan hitam dalam formula pakan ikan yang dapat meningkatkan ketahanan tubuh ikan nila terhadap serangan bakteri A. hydrophila. Penelitian ini menggunakan benih ikan nila dengan ukuran 7-8 cm. Penelitian ini dilaksanakan pada bulan Maret – Mei 2021 dan metode eksperimen yang digunakan adalah model Rancangan Acak Lengkap dengan lima perlakuan dan tiga ulangan. Perlakuan yang digunakan adalah penambahan tepung jintan hitam pada pakan dengan dosis 0 (A), 20 (B), 35 (C) 50 (D) dan 65 (E) g/Kg pakan. Pemeliharaan ikan uji yang diberikan perlakuan yang mengandung tepung jintan hitam dilakukan selama 14 hari, setelah itu dilanjutkan uji tantang menggunakan bakteri A. hydrophila dengan metode immersi selama 14 hari. Bakteri A. hydrophila yang digunakan untuk menginfeksi ikan memiliki kepadatan 108 CFU/mL. Parameter yang digunakan yaitu leukosit, hematokrit, differensial leukosit, gejala klinis, kelangsungan hidup dan kualitas air. Hasil penelitian memperlihatkan tepung jintan hitam dapat meningkatkan ketahanan tubuh ikan nila. Dosis jintan hitam 50 g per 1 kg pakan paling efektif, terlihat dari persentase peningkatan sel darah putih tertinggi sebesar 64.56%, dibandingkan perlakuan B, C, dan E
摘要本研究旨在研究在罗非鱼饲料中添加黑孜然粉，以提高罗非鱼对嗜水拟虫的抗病性。本研究使用罗非鱼幼鱼，尺寸为7 - 8厘米。本研究于2021年3 - 5月进行，实验方法为完全随机设计(CRD)， 5个处理，3个重复。分别在饲料中添加0 (A)、20 (B)、35 (C)、50 (D)和65 (E) g/Kg饲料的黑孜然粉。饲喂添加黑孜然粉的饲粮14 d后，进行嗜水单孢杆菌浸渍攻毒试验14 d。用于感染鱼类的嗜水芽胞杆菌的密度为108 CFU/mL。使用的参数有总白细胞、血细胞比容、总白细胞差、临床症状、存活率和水质。结果表明，黑孜然粉能有效提高罗非鱼的免疫系统。50 g/Kg添加量有效，白细胞增加率最高，为64.56%，而B、C和E处理的白细胞增加率分别为47.40%、48.82%和1.2%。A组白细胞百分率下降19.52%。50 g剂量的黑孜然对罗非鱼鱼苗的红细胞压比和白细胞分化率最高，分别为34%、84.3%、6%和9.7%，恢复时间最快(4 d)，攻毒试验后存活率最高，达95%。关键词:嗜水气单胞菌，罗非鱼幼鱼，黑孜然，免疫系统Penelitian ini mongunakan benikan nila dengan ukuran 7-8 cm。Penelitian ini dilaksanakan pada bulan市场- Mei 2021丹方法，实验yang digunakan adalah模型ranancan Acak Lengkap dengan lima perlakuan dan tiga ulangan。Perlakuan yang digunakan adalah penambahan tepung jintan hitam padpakan dengan剂量0 (A)， 20 (B)， 35 (C) 50 (D)和65 (E) g/Kg pakan。peneliharaan ikan uji yang diberikan perlakuan yang mengandung tepunang jintan hitam dilakukan selama 14 hari, setelah itu dilanjutkan uji tantanang menggunakan bakteri A.亲水细菌登根方法immersi selama 14 hari。嗜水巴克特菌yang digunakan untuk menginfeksi ikan memiliki kepadatan 108 CFU/mL。参数阳地谷那坎白血病、白细胞、差性白血病、格伽拉klinis、克兰松干hidup丹夸利塔空气。Hasil penelitian memperlihatkan tepung jintan hitam dapat meningkatkan kettahanan tubuh ikan nila。剂量金坛hitam 50 g / 1公斤pakan木栅efektif, terlihat达里语persentase peningkatan选取darah putih tertinggi sebesar 64.56%, dibandingkan perlakuan B, C, E丹杨mas mas menunjukan peningkatan选取darah putih sebesar 47.40%, 48.82%丹1.2%。Perlakuan A mengalami penurunan代表了sebesar的19.52%。多斯金坦坦50 g menunjukkan nilai代表酶血液病、差异白质、白斑、白斑、白斑、白斑、白斑、白斑、白斑、白斑、白斑、白斑、白斑、白斑、白斑、白斑、白斑、白斑、白斑、白斑、白斑、白斑、白斑、白斑、白斑、白斑、白斑、白斑、白斑、白斑、白斑、白斑、白斑、白斑、白斑等。嗜水气单胞菌，嗜水气单胞菌，嗜水气单胞菌，嗜水气单胞菌，嗜水气单胞菌，嗜水气单胞菌，嗜水气单胞菌
{"title":"Utilizing of black cumin (Nigella sativa) flour to increase the immunity system of tilapia (Oreochromis niloticus) against Aeromonas hydrophila bacteria attack","authors":"Norma Rizki Silviana, Rosidah, Wahyuniar Pamungkas, Roffi Grandiosa","doi":"10.19027/jai.21.2.161-177","DOIUrl":"https://doi.org/10.19027/jai.21.2.161-177","url":null,"abstract":"ABSTRACT \u0000This study aims to determine dose of black cumin flour in fish feed diets which can improve the disease resistance of tilapia against the attack of A. hydrophila. This study was used tilapia juvenile with a size of 7 – 8 cm. This study was conducted from March – May 2021 and the experimental method is completely randomized design (CRD) with 5 treatments and 3 replications. The treatment used were addition of black cumin flour into fish feed with doses 0 (A), 20 (B), 35 (C), 50 (D), and 65 (E) g/Kg feed. The treatments fed by supplemental diets contained black cumin flour was conducted for 14 days, then a challenge test was carried out with the infection of A. hydrophila bacteria by immersion for14 days. A. hydrophila bacteria used to infect fish had a density of 108 CFU/mL. The Parameters used were total leukocyte, hematocrit, total leukocyte differential, clinical symptoms, survival rate and water quality. The result showed that black cumin flour was effective to increase the immunity system of tilapia. The dose of 50 g/Kg feed was effective showed increase the highest percentage increase in white blood cells of 64.56%, compared to treatments B, C, and E which showed an increase in white blood cells of 47.40%, 48.82% and 1.2%, respectively. Treatment A decreased the percentage of white blood cells by 19.52%. The 50 g dose of black cumin showed the highest percentage value of hematocrit and leukocyte differential in tilapia fry, respectively 34%, lymphocytes 84.3%, monocytes 6%, and neutrophils 9.7% with the fastest recovery period (4 days) and after the challenge test resulted in the highest survival rate of 95%. \u0000Keywords: Aeromonas hydrophila, tilapia juvenile, black cumin, immune system \u0000ABSTRAK \u0000Penelitian ini bertujuan untuk menentukan dosis tepung jintan hitam dalam formula pakan ikan yang dapat meningkatkan ketahanan tubuh ikan nila terhadap serangan bakteri A. hydrophila. Penelitian ini menggunakan benih ikan nila dengan ukuran 7-8 cm. Penelitian ini dilaksanakan pada bulan Maret – Mei 2021 dan metode eksperimen yang digunakan adalah model Rancangan Acak Lengkap dengan lima perlakuan dan tiga ulangan. Perlakuan yang digunakan adalah penambahan tepung jintan hitam pada pakan dengan dosis 0 (A), 20 (B), 35 (C) 50 (D) dan 65 (E) g/Kg pakan. Pemeliharaan ikan uji yang diberikan perlakuan yang mengandung tepung jintan hitam dilakukan selama 14 hari, setelah itu dilanjutkan uji tantang menggunakan bakteri A. hydrophila dengan metode immersi selama 14 hari. Bakteri A. hydrophila yang digunakan untuk menginfeksi ikan memiliki kepadatan 108 CFU/mL. Parameter yang digunakan yaitu leukosit, hematokrit, differensial leukosit, gejala klinis, kelangsungan hidup dan kualitas air. Hasil penelitian memperlihatkan tepung jintan hitam dapat meningkatkan ketahanan tubuh ikan nila. Dosis jintan hitam 50 g per 1 kg pakan paling efektif, terlihat dari persentase peningkatan sel darah putih tertinggi sebesar 64.56%, dibandingkan perlakuan B, C, dan E ","PeriodicalId":32090,"journal":{"name":"Jurnal Akuakultur Indonesia","volume":"125 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76051904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-13DOI: 10.19027/jai.21.2.152-160
Infa Minggawati, Frid Agustinus, Tania Serezova Augusta, Candra Putra Pahawang, Toto Francisco
ABSTRACT This study aimed to determine types, prevalence, and intensity of ectoparasites and endoparasites that infect kerandang fish Channa pleurophtalma and catfish Clarias batrachus. Sampling was carried out in Sebangau River, while identification was carried out at the Fish Quarantine, Quality Control, and Safety Station, Palangka Raya. Examination of ectoparasite infection included bilateral body mucus, caudal fin, and gills. Endoparasite examination was done by observing the fish organs, such as liver, blood, meat, intestines, and stomach. The parasite analysis was determined by calculating the prevalence and intensity. In kerandang fish, the ectoparasites were identified as Trichodina sp., Gyrodactylus sp., Epistylis sp., Dactylogyrus sp., while the endoparasites were Camallanus sp. and Neoechinorhyncus. In catfish, the ectoparasites were identified as Trichodina sp., Dactylogyrus sp., Myxobolus sp., and Costia sp., while the endoparasites were Camallanus sp. Dominant ectoparasite that infected fish was Dactylogyrus sp. on fish gills. For endoparasites, the dominant endoparasites were identified as Neoechinorhyncus in kerandang fish and Camallanus sp. in catfish. The highest prevalence was found in Dactylogyrus sp. at 27%. The prevalence was categorized as a frequent infection. The highest ectoparasite intensity level was obtained from Trichodina sp. at 20.3 ind/fish in kerandang fish and Dactylogyrus sp. at 12.2 ind/fish in catfish. This intensity level is categorized in a medium intensity. Keywords: Channa pleurophtalma, Clarias batrachus, ectoparasites, endoparasites ABSTRAK Penelitian ini bertujuan untuk mengetahui jenis ektoparasit dan endoparasit yang menginfeksi ikan kerandang Channa pleurophtalma dan ikan lele Clarias batrachus serta prevalensi dan intensitas parasitnya. Pengambilan sampel dilakukan di sungai Sebangau sedangkan identifikasi dilaksanakan di Stasiun Karantina Ikan, Pengendalian Mutu dan Keamanan Hasil Perikanan Palangka Raya. Pemeriksaaan infeksi ektoparasit meliputi; lendir tubuh bilateral, sirip ekor, dan insang. Pemeriksaan endoparasit dilakukan dengan cara mengamati bagian organ tubuh ikan seperti hati, darah, daging, usus, dan lambung. Untuk mengetahui tingkat serangan parasit pada ikan dianalisis dengan menghitung prevalensi dan intensitas. Pada ikan kerandang parasit yang teridentifikasi ektoparasit adalah Trichodina sp., Gyrodactylus sp., Epistylis sp., Dactylogyrus sp., dan endoparasit adalah Camallanus sp., dan Neoechinorhyncus. Pada ikan lele parasit ektoparasit yang teridentifikasi adalah Trichodina sp., Dactylogyrus sp., Myxobolus sp., dan Costia sp., sedangkan pada endoparasit adalah Camallanus sp. Ektoparasit yang dominan menginfeksi kedua ikan ini adalah Dactylogyrus yang terdapat pada insang. Sedangkan untuk endoparasit, pada ikan kerandang endoparasit yang dominan adalah Neoechinorhyncus dan endoparasit pada ikan lele adalah Camallanus sp. Prevalensi tertinggi pada ikan kerandang dan ik
摘要:本研究旨在确定感染克兰当鱼(Channa pleurophtalma)和鲶鱼(Clarias batrachus)的外寄生虫和内寄生虫的类型、流行率和强度。在Sebangau河进行了抽样，在Palangka Raya的鱼类检疫、质量控制和安全站进行了鉴定。外寄生虫感染检查包括双侧体粘液、尾鳍和鳃。通过观察鱼的肝、血、肉、肠、胃等脏器进行内寄生虫检查。寄生虫分析通过计算流行率和强度来确定。在克兰当鱼中，外寄生虫鉴定为Trichodina sp.、Gyrodactylus sp.、Epistylis sp.、Dactylogyrus sp.，内寄生虫鉴定为Camallanus sp.和Neoechinorhyncus。鲶鱼体表寄生虫鉴定为Trichodina sp.、Dactylogyrus sp.、Myxobolus sp.和Costia sp.，体表寄生虫鉴定为Camallanus sp.，鱼鳃上的优势体表寄生虫为Dactylogyrus sp.。对内寄生虫，鉴定出优势内寄生虫为克兰塘鱼的Neoechinorhyncus和鲶鱼的Camallanus sp.。Dactylogyrus sp.感染率最高，为27%。流行率被归类为频繁感染。外寄生虫强度最高的是毛霉属，在克兰当鱼中为20.3 ind/条，在鲶鱼中为12.2 ind/条。该强度级别属于中等强度。【关键词】中华绒螯虾、棘螯虾、棘螯虾、棘螯虾、棘螯虾、棘螯虾、棘螯虾。彭甘比兰的样本dilaksanakan di sungai Sebangau sedangkan鉴定为dilaksanakan di Stasiun Karantina Ikan, Pengendalian Mutu dan Keamanan Hasil Perikanan Palangka Raya。美利普氏寄生虫;Lendir tubuh双边，sirip ekor, Dan insang。Pemeriksaan内寄生虫dilakukan dengan cara mengamati bagian organ tububui, kan seperti hati, darah, daging, usus, dan lambung。孟山都、孟山都、孟山都、孟山都、孟山都、孟山都、孟山都、孟山都、孟山都、孟山都、孟山都。adalah内生寄生虫，adalah Camallanus sp.， neechinorhyncus .。寄生蜂，寄生蜂，寄生蜂，寄生蜂，寄生蜂，寄生蜂，寄生蜂，寄生蜂，寄生蜂，寄生蜂，寄生蜂，寄生蜂，寄生蜂，寄生蜂，寄生蜂，寄生蜂，寄生蜂，寄生蜂，寄生蜂，寄生蜂，寄生蜂Sedangkan untuk内寄生虫，paada ikan kerandang内寄生虫yang dominan adalah新人猿内寄生虫paada ikan leele adalah Camallanus sp. Prevalensi tertinggi paada ikan kerandang dankan leele adalah Dactylogyrus yitu masing-masing 27%。Berdasarkan hasil prevalensi parasit pakan kerandang danikkan lelek kedalam kategori infeksi sering。Intensitas tertinggi篇鱼kerandang adalah Trichodina sp. dengan汝20.3印第安纳州/ ekor丹篇鱼乐乐adalah Dactylogyrus sp. dengan汝Intensitas 12.2印第安纳州/ ekor。Dari hasil intensitas parasit padadkedua like an termasuk dalam kategori intensitas sedang。研究方向:胸膜绦虫，棘球绦虫，外寄生物，内寄生物
{"title":"Identification and prevalence of ectoparasites and endoparasites in kerandang fish Channa pleurophtalma and catfish Clarias batrachus captured from Sebangau River","authors":"Infa Minggawati, Frid Agustinus, Tania Serezova Augusta, Candra Putra Pahawang, Toto Francisco","doi":"10.19027/jai.21.2.152-160","DOIUrl":"https://doi.org/10.19027/jai.21.2.152-160","url":null,"abstract":"ABSTRACT \u0000 \u0000This study aimed to determine types, prevalence, and intensity of ectoparasites and endoparasites that infect kerandang fish Channa pleurophtalma and catfish Clarias batrachus. Sampling was carried out in Sebangau River, while identification was carried out at the Fish Quarantine, Quality Control, and Safety Station, Palangka Raya. Examination of ectoparasite infection included bilateral body mucus, caudal fin, and gills. Endoparasite examination was done by observing the fish organs, such as liver, blood, meat, intestines, and stomach. The parasite analysis was determined by calculating the prevalence and intensity. In kerandang fish, the ectoparasites were identified as Trichodina sp., Gyrodactylus sp., Epistylis sp., Dactylogyrus sp., while the endoparasites were Camallanus sp. and Neoechinorhyncus. In catfish, the ectoparasites were identified as Trichodina sp., Dactylogyrus sp., Myxobolus sp., and Costia sp., while the endoparasites were Camallanus sp. Dominant ectoparasite that infected fish was Dactylogyrus sp. on fish gills. For endoparasites, the dominant endoparasites were identified as Neoechinorhyncus in kerandang fish and Camallanus sp. in catfish. The highest prevalence was found in Dactylogyrus sp. at 27%. The prevalence was categorized as a frequent infection. The highest ectoparasite intensity level was obtained from Trichodina sp. at 20.3 ind/fish in kerandang fish and Dactylogyrus sp. at 12.2 ind/fish in catfish. This intensity level is categorized in a medium intensity. \u0000Keywords: Channa pleurophtalma, Clarias batrachus, ectoparasites, endoparasites \u0000 \u0000ABSTRAK \u0000 \u0000Penelitian ini bertujuan untuk mengetahui jenis ektoparasit dan endoparasit yang menginfeksi ikan kerandang Channa pleurophtalma dan ikan lele Clarias batrachus serta prevalensi dan intensitas parasitnya. Pengambilan sampel dilakukan di sungai Sebangau sedangkan identifikasi dilaksanakan di Stasiun Karantina Ikan, Pengendalian Mutu dan Keamanan Hasil Perikanan Palangka Raya. Pemeriksaaan infeksi ektoparasit meliputi; lendir tubuh bilateral, sirip ekor, dan insang. Pemeriksaan endoparasit dilakukan dengan cara mengamati bagian organ tubuh ikan seperti hati, darah, daging, usus, dan lambung. Untuk mengetahui tingkat serangan parasit pada ikan dianalisis dengan menghitung prevalensi dan intensitas. Pada ikan kerandang parasit yang teridentifikasi ektoparasit adalah Trichodina sp., Gyrodactylus sp., Epistylis sp., Dactylogyrus sp., dan endoparasit adalah Camallanus sp., dan Neoechinorhyncus. Pada ikan lele parasit ektoparasit yang teridentifikasi adalah Trichodina sp., Dactylogyrus sp., Myxobolus sp., dan Costia sp., sedangkan pada endoparasit adalah Camallanus sp. Ektoparasit yang dominan menginfeksi kedua ikan ini adalah Dactylogyrus yang terdapat pada insang. Sedangkan untuk endoparasit, pada ikan kerandang endoparasit yang dominan adalah Neoechinorhyncus dan endoparasit pada ikan lele adalah Camallanus sp. Prevalensi tertinggi pada ikan kerandang dan ik","PeriodicalId":32090,"journal":{"name":"Jurnal Akuakultur Indonesia","volume":"135 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86299760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-05DOI: 10.19027/jai.21.2.142-151
A. N. Putra, M. B. Syamsunarno, D. Rohayati, Yuhana Marta Sarah, Ghulam Abdul Matien
Rice bran is being fermented using Aspergillus niger in order to increase its usage as a raw material for the catfish diet. This research aims to evaluate the potentials of rice bran fermented with A. niger to replace soybean meal in catfish Clarias sp diet. There were two research stages, first the fermentation test of A. niger on rice bran that used a completely randomized factorial design consisting of 2 factors, namely the dose of A. niger (0, 0.5, 1, 1.5, and 2 g/100 g) and incubation time (0. 24 and 48 hours). Secondly, the substitution test of soybean meal with fermented rice bran on five different of substitution levels (0, 15, 20, 35, and 40%) in the catfish diet. The results showed that the fermentation of A. niger of 2 % with an incubation period of 24 hours in rice bran resulted in the smallest crude fiber and fraction content, and increased protein and amino acid including serine, threonine, alanine, methionine, isoleucine, and lysine. The specific growth rate in the substitution treatment of 0-20% was significantly (P<0.05) higher than that of 35-40%. There was also no difference (P>0.05) in the growth performance of catfish in the 0 to 20% substitution treatment. Also, replacing soybean meal with fermented rice bran up to 20% did not have a negative effect on the growth performance of catfish. It was concluded that fermented rice bran could be used as raw material for catfish.
{"title":"Evaluation of fermented rice bran to replace soybean meal in the diet of Clarias sp. fingerling","authors":"A. N. Putra, M. B. Syamsunarno, D. Rohayati, Yuhana Marta Sarah, Ghulam Abdul Matien","doi":"10.19027/jai.21.2.142-151","DOIUrl":"https://doi.org/10.19027/jai.21.2.142-151","url":null,"abstract":"Rice bran is being fermented using Aspergillus niger in order to increase its usage as a raw material for the catfish diet. This research aims to evaluate the potentials of rice bran fermented with A. niger to replace soybean meal in catfish Clarias sp diet. There were two research stages, first the fermentation test of A. niger on rice bran that used a completely randomized factorial design consisting of 2 factors, namely the dose of A. niger (0, 0.5, 1, 1.5, and 2 g/100 g) and incubation time (0. 24 and 48 hours). Secondly, the substitution test of soybean meal with fermented rice bran on five different of substitution levels (0, 15, 20, 35, and 40%) in the catfish diet. The results showed that the fermentation of A. niger of 2 % with an incubation period of 24 hours in rice bran resulted in the smallest crude fiber and fraction content, and increased protein and amino acid including serine, threonine, alanine, methionine, isoleucine, and lysine. The specific growth rate in the substitution treatment of 0-20% was significantly (P<0.05) higher than that of 35-40%. There was also no difference (P>0.05) in the growth performance of catfish in the 0 to 20% substitution treatment. Also, replacing soybean meal with fermented rice bran up to 20% did not have a negative effect on the growth performance of catfish. It was concluded that fermented rice bran could be used as raw material for catfish.","PeriodicalId":32090,"journal":{"name":"Jurnal Akuakultur Indonesia","volume":"11 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88545210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-08-31DOI: 10.19027/jai.21.2.133-141
I. Diatin, Irzal Effendi, Y. Hadiroseyani, T. Budiardi, Virta Rizki Hernanda, Nidwidyanthi Nidwidyanthi, Apriana Vinasyiam
Indonesia merupakan salah satu negara yang melakukan ekspor lobster ke negara Hongkong, Vietnam, China, dan Singapura, dengan nilai ekspor lobster Indonesia mengalami rata-rata pertumbuhan 3,54% pertahun. Produksi lobster tersebut masih mengandalkan hasil tangkapan, karena budidaya lobster belum banyak dilakukan di Indonesia. Pantai Barat Provinsi Lampung dikenal sebagai daerah tangkapan lobster yang dimanfaatkan oleh nelayan setempat sebagai sumber pendapatan utama. Untuk mengetahui potensi ekonomi dari budidaya lobster, maka dilakukan penelitian dengan tujuan menganalisis ketersediaan benih bening lobster (BBL) dan rantai pasok lobster sebagai suplai benih untuk kebutuhan budidaya lobster. Metode penelitian ini dilakukan dengan metode survei di Kecamatan Krui Selatan, Kabupaten Pesisir Barat, Lampung. Responden dalam penelitian adalah para nelayan penangkap BBL dan pengepul BBL. Penentuan responden dilakukan dengan metode snowball sampling. Hasil penelitian menunjukkan bahwa jenis lobster utama yang ditangkap adalah jenis lobster pasir dan lobster Mutiara. Hasil tangkapan nelayan lobster pada bulan gelap yaitu rata-rata 121 340 ekor BBL/bulan, sedangkan pada bulan terang rata-rata 4 680 ekor BBL/bulan, sehinnga dalam setahun benih yang dihasilkan mencapai 1.500.000 ekor BBL. BBL hasil tangkapan nelayan akan dijual pengepul kecil, selanjutnya ke pengepul besar, distributor dan eksportir. BBL terdistribusi ke berbagai wilayah mencakup Lampung, Bengkulu, Jambi, Palembang dan Jakarta. Marjin pemasaran yang diperoleh pengepul kecil dan besar mencapai 15 %. Produksi benih lobster yang melimpah di perairan Pantai Pesisir Barat Lampung sangat berpotensi untuk pengembangan budidaya pendederan lobster sebagai sumber ekonomi baru.
{"title":"Analisis Ketersediaan Benih Bening Lobster (BBL) Hasil Tangkapan Alam untuk Kegiatan Budidaya Pendederan Lobster","authors":"I. Diatin, Irzal Effendi, Y. Hadiroseyani, T. Budiardi, Virta Rizki Hernanda, Nidwidyanthi Nidwidyanthi, Apriana Vinasyiam","doi":"10.19027/jai.21.2.133-141","DOIUrl":"https://doi.org/10.19027/jai.21.2.133-141","url":null,"abstract":"Indonesia merupakan salah satu negara yang melakukan ekspor lobster ke negara Hongkong, Vietnam, China, dan Singapura, dengan nilai ekspor lobster Indonesia mengalami rata-rata pertumbuhan 3,54% pertahun. Produksi lobster tersebut masih mengandalkan hasil tangkapan, karena budidaya lobster belum banyak dilakukan di Indonesia. Pantai Barat Provinsi Lampung dikenal sebagai daerah tangkapan lobster yang dimanfaatkan oleh nelayan setempat sebagai sumber pendapatan utama. Untuk mengetahui potensi ekonomi dari budidaya lobster, maka dilakukan penelitian dengan tujuan menganalisis ketersediaan benih bening lobster (BBL) dan rantai pasok lobster sebagai suplai benih untuk kebutuhan budidaya lobster. Metode penelitian ini dilakukan dengan metode survei di Kecamatan Krui Selatan, Kabupaten Pesisir Barat, Lampung. Responden dalam penelitian adalah para nelayan penangkap BBL dan pengepul BBL. Penentuan responden dilakukan dengan metode snowball sampling. Hasil penelitian menunjukkan bahwa jenis lobster utama yang ditangkap adalah jenis lobster pasir dan lobster Mutiara. Hasil tangkapan nelayan lobster pada bulan gelap yaitu rata-rata 121 340 ekor BBL/bulan, sedangkan pada bulan terang rata-rata 4 680 ekor BBL/bulan, sehinnga dalam setahun benih yang dihasilkan mencapai 1.500.000 ekor BBL. BBL hasil tangkapan nelayan akan dijual pengepul kecil, selanjutnya ke pengepul besar, distributor dan eksportir. BBL terdistribusi ke berbagai wilayah mencakup Lampung, Bengkulu, Jambi, Palembang dan Jakarta. Marjin pemasaran yang diperoleh pengepul kecil dan besar mencapai 15 %. Produksi benih lobster yang melimpah di perairan Pantai Pesisir Barat Lampung sangat berpotensi untuk pengembangan budidaya pendederan lobster sebagai sumber ekonomi baru.","PeriodicalId":32090,"journal":{"name":"Jurnal Akuakultur Indonesia","volume":"69 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82858194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-08-30DOI: 10.19027/jai.21.2.118-124
Amalia Sutriana, Ahmad Baihaqi, Iwan Hasri, Nurliana, Hafizuddin Hafizuddin
ABSTRACT The aim of this study was to analyze the effect of spawnprim hormone induction with different doses on gonadal maturity of tinfoil barb fish broodstock (Barbonymus schwanenfeldii). A completely randomized design (CRD) was used in this study with 4 treatments and 3 replications. Fish broodstock in group A (control) was not given any treatment, while fish broodstocks in groups B, C, and D were injected with spawnprim hormone at doses of 0.3 ml/kg body weight, 0.6 ml/kg body weight, and 0.9 ml/kg body weight, respectively. The measured parameters were gonad maturity level, broodstock weight gain, egg diameter, and fecundity. Data were analyzed using one way analysis of variance (ANOVA). The result showed that the gonad maturity level (GML) in groups B, C, and D (GML III and IV) was better than group A (GML I). Statistical analysis showed that the induction of spawnprim hormone on tinfoil barb broodstocks significantly affect (P<0.05) broodstock weight gain, egg diameter, and fecundity. The optimal dose of spawnprim hormone for tinfoil barb was 0.6 ml/kg body weight, with the average broodstock weight gain, egg diameter, and fecundity were 0.011 kg, 1.55 mm, and 102.15 eggs, respectively. The induction of spawnprim hormone has a positive effect on gonad maturation of tinfoil barb fish (B. schwanenfeldii), with the optimum dose is 0.6 ml/kg fish Keywords: gonad maturity, tinfoil barb fish, spawnprim ABSTRAK Penelitian ini bertujuan untuk menganalisis pengaruh penggunaan hormon spawnprim dengan dosis yang berbeda terhadap kematangan gonad induk ikan lemeduk Barbonymus schwanenfeldii. Metode yang digunakan pada penelitian ini adalah metode rancangan acak lengkap (RAL) dengan 4 kelompok perlakuan dan 3 kali pengulangan. Ikan pada kelompok A (kontrol) tidak diberikan perlakuan, sedangkan ikan pada kelompok B, C, dan D masing-masing diinjeksi hormon spawnprim dengan dosis 0.3 ml/kg bobot badan; 0.6 ml/kg bobot badan; dan 0,9 ml/kg bobot badan. Parameter yang diamati adalah persentase induk matang gonad akhir, pertambahan bobot induk, pertambahan diameter telur, dan fekunditas. Data dianalisis dengan menggunakan analisis varians (ANAVA) pola satu arah. Hasil penelitian menunjukkan tingkat kematangan gonad (TKG) pada kelompok perlakuan B, C, dan D (TKG III dan IV) lebih baik dari kelompok A (TKG I). Hasil uji ANAVA menunjukkan bahwa pemberian hormon spawnprim pada induk ikan lemeduk berpengaruh nyata (P<0.05) terhadap pertambahan bobot induk, pertambahan diameter telur, dan fekunditas. Tingkat kematangan gonad terbaik pada penelitian ini diperoleh pada kelompok perlakuan C yang diinduksi hormon spawnprim dengan dosis 0.6 ml/kg bobot badan induk ikan dengan rata - rata pertambahan bobot induk, pertambahan diameter telur, dan fekunditas masing-masing adalah 0.011 kg, 1.55 mm, dan 102.15 butir telur. Induksi hormon spawnprim menimbulkan pengaruh yang positif terhadap pertambahan bobot induk, diameter telur dan fekunditas ikan lemeduk (B
摘要本研究旨在分析不同剂量的卵泡激素诱导对锡箔倒钩鱼(Barbonymus schwanenfeldii)种鱼性腺成熟的影响。本研究采用完全随机设计(CRD)， 4个处理，3个重复。A组(对照)不进行任何处理，B组、C组和D组分别按0.3 ml/kg体重、0.6 ml/kg体重和0.9 ml/kg体重注射促卵黄激素。测定的参数为性腺成熟度、亲鱼增重、卵径和繁殖力。数据分析采用单因素方差分析(ANOVA)。结果表明，B、C和D组(GML III和IV组)的性腺成熟度水平(GML)均优于A组(GML I组)。统计分析表明，诱导产蛋激素对锡纸钩鱼的增重、卵径和繁殖力有显著影响(P<0.05)。锡箔倒钩产卵激素的最佳用量为0.6 ml/kg体重，平均增重0.011 kg，平均卵径1.55 mm，平均产卵量102.15个卵。诱导产蛋激素对锡纸倒钩鱼(B. schwanenfeldii)性腺成熟有积极影响，最佳剂量为0.6 ml/kg鱼。关键词:性腺成熟，锡纸倒钩鱼，产卵激素，产卵激素，雌雄同体，雌雄同体。4 . kelompok . perlakuan . 3 . kali penpangangan。Ikan pakadkelpok A(对照)titak diberikan perlakuan, sedangkan pakadkelpok B, C, dan D - masing-masing diinjeksi激素产卵量0.3 ml/kg bobot badan;巴丹0.6 ml/kg;丹0、9 ml/kg bobot巴丹。参数yang diamati adalah代表酶induk matang性腺akhir, pertambahan bobot induk, pertambahan直径telur, danfekunditas。数据分析:登甘蒙古纳坎分析变异数(ANAVA)。Hasil penelitian menunjukkan tingkat kematangan gonad (TKG) pada kelompok perlakuan B, C, dan D (TKG III daniv) lebih baik dari kelompok A (TKG I). Hasil uji ANAVA menunjukkan bahwa pemberian hormon产卵prim pada induk ikan lemeduk berpengaruh nyata (P<0.05) terhadap pertambahan bobot induk, pertambahan直径telur, dan fekunditas。Tingkat kematangan gonad terbaik pada penelitian ini diperoleh pada kelompok perlakuan C yang diinduksi激素产卵量0.6 ml/kg bobodan danan - rata - rata pertambahan boboduk, pertambahan直径长度，dan fekunditas masing- masalah 0.011 kg, 1.55 mm, dan 102.15但长度。赤潮激素产卵量为0.6 ml/kg，赤潮激素产卵量为0.6 ml/kg，赤潮激素产卵量为0.6 ml/kg，赤潮激素产卵量为0.6 ml/kg。Kata kunci: kematangan性腺，ikkan lemeduk，产卵
{"title":"Hormonal induction of gonad maturation in female tinfoil barb fish (Barbonymus schwanenfeldii) using spawnprim hormone","authors":"Amalia Sutriana, Ahmad Baihaqi, Iwan Hasri, Nurliana, Hafizuddin Hafizuddin","doi":"10.19027/jai.21.2.118-124","DOIUrl":"https://doi.org/10.19027/jai.21.2.118-124","url":null,"abstract":"ABSTRACT \u0000 \u0000The aim of this study was to analyze the effect of spawnprim hormone induction with different doses on gonadal maturity of tinfoil barb fish broodstock (Barbonymus schwanenfeldii). A completely randomized design (CRD) was used in this study with 4 treatments and 3 replications. Fish broodstock in group A (control) was not given any treatment, while fish broodstocks in groups B, C, and D were injected with spawnprim hormone at doses of 0.3 ml/kg body weight, 0.6 ml/kg body weight, and 0.9 ml/kg body weight, respectively. The measured parameters were gonad maturity level, broodstock weight gain, egg diameter, and fecundity. Data were analyzed using one way analysis of variance (ANOVA). The result showed that the gonad maturity level (GML) in groups B, C, and D (GML III and IV) was better than group A (GML I). Statistical analysis showed that the induction of spawnprim hormone on tinfoil barb broodstocks significantly affect (P<0.05) broodstock weight gain, egg diameter, and fecundity. The optimal dose of spawnprim hormone for tinfoil barb was 0.6 ml/kg body weight, with the average broodstock weight gain, egg diameter, and fecundity were 0.011 kg, 1.55 mm, and 102.15 eggs, respectively. The induction of spawnprim hormone has a positive effect on gonad maturation of tinfoil barb fish (B. schwanenfeldii), with the optimum dose is 0.6 ml/kg fish \u0000 \u0000Keywords: gonad maturity, tinfoil barb fish, spawnprim \u0000 \u0000ABSTRAK \u0000 \u0000Penelitian ini bertujuan untuk menganalisis pengaruh penggunaan hormon spawnprim dengan dosis yang berbeda terhadap kematangan gonad induk ikan lemeduk Barbonymus schwanenfeldii. Metode yang digunakan pada penelitian ini adalah metode rancangan acak lengkap (RAL) dengan 4 kelompok perlakuan dan 3 kali pengulangan. Ikan pada kelompok A (kontrol) tidak diberikan perlakuan, sedangkan ikan pada kelompok B, C, dan D masing-masing diinjeksi hormon spawnprim dengan dosis 0.3 ml/kg bobot badan; 0.6 ml/kg bobot badan; dan 0,9 ml/kg bobot badan. Parameter yang diamati adalah persentase induk matang gonad akhir, pertambahan bobot induk, pertambahan diameter telur, dan fekunditas. Data dianalisis dengan menggunakan analisis varians (ANAVA) pola satu arah. Hasil penelitian menunjukkan tingkat kematangan gonad (TKG) pada kelompok perlakuan B, C, dan D (TKG III dan IV) lebih baik dari kelompok A (TKG I). Hasil uji ANAVA menunjukkan bahwa pemberian hormon spawnprim pada induk ikan lemeduk berpengaruh nyata (P<0.05) terhadap pertambahan bobot induk, pertambahan diameter telur, dan fekunditas. Tingkat kematangan gonad terbaik pada penelitian ini diperoleh pada kelompok perlakuan C yang diinduksi hormon spawnprim dengan dosis 0.6 ml/kg bobot badan induk ikan dengan rata - rata pertambahan bobot induk, pertambahan diameter telur, dan fekunditas masing-masing adalah 0.011 kg, 1.55 mm, dan 102.15 butir telur. Induksi hormon spawnprim menimbulkan pengaruh yang positif terhadap pertambahan bobot induk, diameter telur dan fekunditas ikan lemeduk (B","PeriodicalId":32090,"journal":{"name":"Jurnal Akuakultur Indonesia","volume":"4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77078013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-08-30DOI: 10.19027/jai.21.2.125-132
Munti Yuhana, Andreas Tambun, W. Widanarni, U. Afiff
This study aimed to evaluate the effectivity of microencapsulated synbiotic (MS), Bacillus sp. NP5 and mannan oligosaccharide (MOS) dietary in different feeding frequencies in Pacific white shrimp culture field experiment. The MS was administered as a feed supplementation to enhance the immunity for prevention against co-infection with WSSV (White Spot Syndrome Virus) and Vibrio harveyi. The synbiotic was microencapsulated by the spray dryer method. Shrimps were reared in the floating net cages in the pond. Treatments included the administration of MS at different frequencies i.e, daily (A), twice a week (B), once a week (C), and without MS supplementation (consisted of negative and positive controls) with a feeding rate of 6% of shrimp biomass (5 times a day). During the challenge trial, shrimps were removed and further reared in plastic tanks, for 7 days. The shrimps (except negative control treatment) were intramuscularly injected by WSSV filtrate at the infective dosage of 10-4 copies.ml-1. Twenty four hours after WSSV injection the shrimps were immersed in the water-containing cells suspension of V. harveyi at the cell’s population dosage of 106 CFU.ml-1. Immune responses were observed for 7 days after experimental infection. The shrimps that have been treated with daily MS supplementation (A) showed better immune responses i.e., total haemocyte counts, phenoloxidase, respiratory burst, and the lower pathogenic cells abundance in the intestine compared to other treatments groups.
{"title":"Field Experiment: Synbiotic Microcapsule Dietary Supplementation Improved the Health Status of the White Shrimp under the Challenge of Coinfection Diseases through Improvement the Beneficial Microbial Composition.","authors":"Munti Yuhana, Andreas Tambun, W. Widanarni, U. Afiff","doi":"10.19027/jai.21.2.125-132","DOIUrl":"https://doi.org/10.19027/jai.21.2.125-132","url":null,"abstract":"This study aimed to evaluate the effectivity of microencapsulated synbiotic (MS), Bacillus sp. NP5 and mannan oligosaccharide (MOS) dietary in different feeding frequencies in Pacific white shrimp culture field experiment. The MS was administered as a feed supplementation to enhance the immunity for prevention against co-infection with WSSV (White Spot Syndrome Virus) and Vibrio harveyi. The synbiotic was microencapsulated by the spray dryer method. Shrimps were reared in the floating net cages in the pond. Treatments included the administration of MS at different frequencies i.e, daily (A), twice a week (B), once a week (C), and without MS supplementation (consisted of negative and positive controls) with a feeding rate of 6% of shrimp biomass (5 times a day). During the challenge trial, shrimps were removed and further reared in plastic tanks, for 7 days. The shrimps (except negative control treatment) were intramuscularly injected by WSSV filtrate at the infective dosage of 10-4 copies.ml-1. Twenty four hours after WSSV injection the shrimps were immersed in the water-containing cells suspension of V. harveyi at the cell’s population dosage of 106 CFU.ml-1. Immune responses were observed for 7 days after experimental infection. The shrimps that have been treated with daily MS supplementation (A) showed better immune responses i.e., total haemocyte counts, phenoloxidase, respiratory burst, and the lower pathogenic cells abundance in the intestine compared to other treatments groups.","PeriodicalId":32090,"journal":{"name":"Jurnal Akuakultur Indonesia","volume":"50 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79386331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-07-27DOI: 10.19027/jai.21.2.109-127
T. Budiardi, I. Effendi, Muhammad Aghistni Rahman, Apriana Vinasyiam
The growth rate highly varies in nurseries of eel. Variations in size lead to competition in obtaining feed, this causes stunting of smaller fish. This situation leads to high production costs due to poor feed utilization efficiency. Grading needs to be done periodically to improve nursery production performance. Water quality is controlled by a recirculation system that can support production performance through the degradation of toxic compounds. This study aims to analyze the production and nursery performance of graded eel (Anguilla bicolor bicolor) that graded in the same batch in a recirculation system. Completely randomized design (CRD) consisting of three treatments with four replications was used in this research. The treatments included nursery of graded eels for 60 days in three groups of initial weight size, namely 0,35±0,00 g (A); 0,50±0,00 g (B); and 1,04±0,00 g (C). There were 344 eels (A), 239 eels (B), and 116 eels (C) in each replication. The best nursery production performance was obtained in the treatment of 1,04±0,00 g, and the best nursery business performance was obtained in the treatment of 0,50±0,00 g.
鳗鲡苗圃的生长速率差异很大。体型的差异导致了获取饲料的竞争，这导致了较小的鱼类发育迟缓。这种情况导致饲料利用效率低，生产成本高。分级需要定期进行，以提高苗圃生产性能。水质由再循环系统控制，该系统可以通过降解有毒化合物来支持生产性能。本研究旨在分析在循环系统中同一批次分级鳗鲡(Anguilla bicolor bicolor)的生产和苗圃性能。本研究采用完全随机设计(CRD)，包括3个处理，4个重复。按初始体重分别为0、35±0 000 g (A) 3组，分级育苗60 d;0.50±0.000 g (B);和1,04±0,000 g (C)。每个复制中分别有344条(A)、239条(B)和116条(C)鳗鱼。苗圃生产性能在1.04±0万g处理下达到最佳，苗圃经营性能在0.50±0万g处理下达到最佳。
{"title":"KINERJA PRODUKSI PENDEDERAN IKAN SIDAT Anguilla bicolor bicolor HASIL GRADING PADA SISTEM RESIRKULASI","authors":"T. Budiardi, I. Effendi, Muhammad Aghistni Rahman, Apriana Vinasyiam","doi":"10.19027/jai.21.2.109-127","DOIUrl":"https://doi.org/10.19027/jai.21.2.109-127","url":null,"abstract":"The growth rate highly varies in nurseries of eel. Variations in size lead to competition in obtaining feed, this causes stunting of smaller fish. This situation leads to high production costs due to poor feed utilization efficiency. Grading needs to be done periodically to improve nursery production performance. Water quality is controlled by a recirculation system that can support production performance through the degradation of toxic compounds. This study aims to analyze the production and nursery performance of graded eel (Anguilla bicolor bicolor) that graded in the same batch in a recirculation system. Completely randomized design (CRD) consisting of three treatments with four replications was used in this research. The treatments included nursery of graded eels for 60 days in three groups of initial weight size, namely 0,35±0,00 g (A); 0,50±0,00 g (B); and 1,04±0,00 g (C). There were 344 eels (A), 239 eels (B), and 116 eels (C) in each replication. The best nursery production performance was obtained in the treatment of 1,04±0,00 g, and the best nursery business performance was obtained in the treatment of 0,50±0,00 g.","PeriodicalId":32090,"journal":{"name":"Jurnal Akuakultur Indonesia","volume":"62 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87148790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-07-25DOI: 10.19027/jai.21.2.93-108
Mahatma Kresnha Radityo, Rosidah, Walim Lili, Roffi Grandiosa Herman
This research aims to determine the optimal dose of chitosan that is appropriate to be given to commercial feed in increasing the immune system of sangkuriang catfish fry. The research was carried out in April – June 2021 at the Aquaculture Laboratory and Biotechnology Laboratory, Faculty of Fisheries and Marine Sciences, Padjadjaran University. This research used an experimental method of Completely Randomized Design (CRD) with five treatments and three replications. The treatment used was the addition of chitosan to feed with doses of 0 g (A), 2.5 g (B), 5 g (C), 7.5 g (D), and 10 g (E). Maintenance of test fish for 14 days. Experimental fish were infected by Aeromonas hydrophila bacteria with a density of 108 CFU/ml with 10 ml immersion method. Parameters observed included white blood cells, leukocyte differential, clinical symptoms, survival and water quality. Data on white blood cell count, and survival were analyzed by ANOVA, if there was a significant difference, Duncan's test was performed. Leukocyte differential data, clinical symptoms and water quality were analyzed descriptively. The results showed that the addition of chitosan at a dose of 10 g was effective for improving the immune system performance of catfish fry. During 14 days of chitosan administration, the white blood cell count increased by 41.60% from the value of 13.96 × 104 cells/mm3 to 23.90 × 104 cells/mm3. The 10 g chitosan treatment was an effective dose to stimulate differential leukocytes in catfish to improve the immune system, it was also marked by the fastest clinical symptom recovery period and the highest survival rate after the challenge test, which was 96.6%.
本研究旨在确定商品饲料中适宜添加壳聚糖以提高桑库良鲶鱼鱼苗免疫系统的最佳剂量。该研究于2021年4月至6月在Padjadjaran大学渔业和海洋科学学院水产养殖实验室和生物技术实验室进行。本研究采用完全随机设计(CRD)试验方法，5个处理，3个重复。在饲料中添加壳聚糖，剂量分别为0 g (A)、2.5 g (B)、5 g (C)、7.5 g (D)和10 g (E)。试验鱼维持14天。实验鱼采用10ml浸渍法感染嗜水气单胞菌，感染密度为108 CFU/ml。观察指标包括白细胞、白细胞分化、临床症状、存活率和水质。白细胞计数和生存率数据采用方差分析，如有显著差异，进行邓肯检验。描述性分析白细胞鉴别数据、临床症状和水质。结果表明，添加10 g剂量的壳聚糖可有效提高鲶鱼鱼苗的免疫系统性能。在给药14 d期间，白细胞计数从13.96 × 104细胞/mm3增加到23.90 × 104细胞/mm3，增加了41.60%。10 g壳聚糖是刺激鲶鱼体内分化白细胞改善免疫系统的有效剂量，其临床症状恢复期最快，攻毒试验后存活率最高，为96.6%。
{"title":"The Effectiveness of Chitosan to Increase Body Resistance of Sangkuriang Catfish (Clarias gariepinus) Against Aeromonas hydrophila Attack","authors":"Mahatma Kresnha Radityo, Rosidah, Walim Lili, Roffi Grandiosa Herman","doi":"10.19027/jai.21.2.93-108","DOIUrl":"https://doi.org/10.19027/jai.21.2.93-108","url":null,"abstract":"This research aims to determine the optimal dose of chitosan that is appropriate to be given to commercial feed in increasing the immune system of sangkuriang catfish fry. The research was carried out in April – June 2021 at the Aquaculture Laboratory and Biotechnology Laboratory, Faculty of Fisheries and Marine Sciences, Padjadjaran University. This research used an experimental method of Completely Randomized Design (CRD) with five treatments and three replications. The treatment used was the addition of chitosan to feed with doses of 0 g (A), 2.5 g (B), 5 g (C), 7.5 g (D), and 10 g (E). Maintenance of test fish for 14 days. Experimental fish were infected by Aeromonas hydrophila bacteria with a density of 108 CFU/ml with 10 ml immersion method. Parameters observed included white blood cells, leukocyte differential, clinical symptoms, survival and water quality. Data on white blood cell count, and survival were analyzed by ANOVA, if there was a significant difference, Duncan's test was performed. Leukocyte differential data, clinical symptoms and water quality were analyzed descriptively. The results showed that the addition of chitosan at a dose of 10 g was effective for improving the immune system performance of catfish fry. During 14 days of chitosan administration, the white blood cell count increased by 41.60% from the value of 13.96 × 104 cells/mm3 to 23.90 × 104 cells/mm3. The 10 g chitosan treatment was an effective dose to stimulate differential leukocytes in catfish to improve the immune system, it was also marked by the fastest clinical symptom recovery period and the highest survival rate after the challenge test, which was 96.6%.","PeriodicalId":32090,"journal":{"name":"Jurnal Akuakultur Indonesia","volume":"94 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79222527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Muhammad Arif Mulya, Fachriyan Hasmi Pasaribu, Usamah Afiff, Munti Yuhana
ABSTRACT This study aimed to conduct the characterization and molecular detection of the pathogenicity and antibiotic-resistance genes in Vibrio parahaemolyticus, as the causative agent of vibriosis in Pacific white shrimp. The V. parahaemolyticus isolates were collected from the shrimp’s hepatopancreas, before biochemical test and polymerase chain reaction (PCR) sequencing of the 16S rRNA gene confirmation. The hemolysis test and PCR were applied to detect the presence of virulence genes, namely toxR, thermostable direct haemolysin (tdh), and tdh-related haemolysin (trh). The Kirby-Bauer method was used for characterizing the resistance patterns against ampicillin (AMP), tetracycline (TET), cyprofloxacin (CIP), enrofloxacin (ENR), and chloramphenicol (CHL). The biochemical tests and PCR-16SrRNA gene sequencing confirmed that 12 isolates belonged to V. parahaemolyticus that were further verified by amplification of the toxR gene in 382 bp (100% of the isolates). The alpha hemolysis activity was also confirmed by the amplicon of 199 bp in all isolates. All V. parahaemolyticus isolates showed their resistance to AMP and 42% of the isolates were TET-resistant. However, no resistance was shown to CIP, ENR, and CHL. The PCR-based analysis resulted a detectable resistance gene of ampC (42% of the isolates) and tetB (83% of the isolates). Keywords: antibiotics, shrimp, resistance, virulency, Vibrio parahaemolyticus ABSTRAK Penelitian ini bertujuan untuk melakukan karakterisasi dan deteksi molekular dari gen patogenisitas dan resistansi antibiotik pada Vibrio parahaemolyticus, agen penyebab vibriosis pada udang vaname. Isolat V. parahaemolyticus dikoleksi dari hepatopankreas, diuji secara biokimiawi dan selanjutnya dikonfirmasi dengan polymerase chain reaction (PCR)-sekuensing dari gen 16S rRNA. Tes hemolisis dan metode PCR diterapkan untuk mendeteksi keberadaan gen virulensi toxR, thermostable direct haemolysin (tdh) and tdh-related haemolysin (trh). Metode Kirby Bauer digunakan untuk karakterisasi pola resistansi terhadap ampisilin (AMP), tetrasiklin (TET), kloramfenikol (CHL), siprofloksasin (CIP) dan enrofloksasin (ENR). Uji biokimia dan sekuensing gen PCR-16SrRNA memastikan bahwa 12 isolat adalah V. parahaemolyticus yang selanjutnya diverifikasi dengan amplifikasi gen toxR berukuran 382 bp (100% isolat). Aktivitas alfa hemolisis juga dikonfirmasi dengan amplikon PCR (199 bp) di semua isolat. Seluruh isolat V. parahaemolyticus menunjukkan resistansinya terhadap AMP, 42% resistan TET, tidak ada resistansi yang ditunjukkan pada CIP, ENR dan CHL. Analisis berbasis PCR menghasilkan gen resistan yang terdeteksi dari gen ampC (42% isolat) dan gen tetB (83% isolat). Kata kunci: Antibiotik, udang, resistansi, virulensi, Vibrio parahaemolyticus
摘要本研究旨在对太平洋白对虾弧菌病病原副溶血性弧菌的致病性和耐药基因进行鉴定和分子检测。从对虾肝胰脏中采集副溶血性弧菌分离株，进行生化检测和16S rRNA基因测序确认。采用溶血试验和PCR检测毒力基因toxR、耐热直接溶血素(tdh)和tdh相关溶血素(trh)的存在。采用Kirby-Bauer法鉴定菌株对氨苄西林(AMP)、四环素(TET)、环丙沙星(CIP)、恩诺沙星(ENR)和氯霉素(CHL)的耐药模式。生化试验和PCR-16SrRNA基因测序证实12株分离株为副溶血性弧菌，并扩增382 bp(100%)毒株的toxR基因。所有分离株的α溶血活性扩增量均为199 bp。所有副溶血性弧菌分离株均对AMP耐药，42%的分离株对tet耐药。然而，对CIP、ENR和CHL没有显示出耐药性。基于pcr的分析结果检测到ampC(42%)和tetB(83%)的耐药基因。【关键词】抗生素，对虾，耐药性，毒力，副溶血性弧菌分离株副溶血性弧菌双孔氏肝胰腺、双孔氏弧菌生物克隆、双孔氏弧菌登根聚合酶链反应(PCR)-双孔氏弧菌16S rRNA。溶血方法PCR检测了溶血酶、毒杆菌、抗热直接溶血素(tdh)和tdh相关溶血素(trh)。方法Kirby Bauer digunakan untuk karakterisasi耐药的抗菌药物有:抗菌氨苄西林(AMP)、四环素(TET)、氯氨苯尼科尔(CHL)、siprofloksasin (CIP)和enrofloksasin (ENR)。Uji biokimia dan sekuensing gen PCR-16SrRNA memastikan bahwa 12 isolat adalah V.副溶血性V. yang selanjutnya diverfikasi dengan amplifikasi gen toxR berukuran 382 bp(100%分离)。α活血菌(Aktivitas alfa hemisjuga dikonfirmasi dengan扩增子PCR (199 bp))中Seluruh isolat相关拮抗menunjukkan resistansinya terhadap AMP, 42%阻力春节,有些ada resistansi杨ditunjukkan篇CIP, ENR丹的背影。基于PCR分析，分离得到蒙哈西坎根耐药菌ampC(42%)和根tetB(83%)。Kata kunci:抗生素，udang，耐药，病毒，副溶血性弧菌
{"title":"Characterization and molecular detection of pathogenicity and antibioticresistance genes in Vibrio parahaemolyticus isolated from pacific white shrimp","authors":"Muhammad Arif Mulya, Fachriyan Hasmi Pasaribu, Usamah Afiff, Munti Yuhana","doi":"10.19027/jai.21.1.81-92","DOIUrl":"https://doi.org/10.19027/jai.21.1.81-92","url":null,"abstract":"ABSTRACT \u0000 \u0000This study aimed to conduct the characterization and molecular detection of the pathogenicity and antibiotic-resistance genes in Vibrio parahaemolyticus, as the causative agent of vibriosis in Pacific white shrimp. The V. parahaemolyticus isolates were collected from the shrimp’s hepatopancreas, before biochemical test and polymerase chain reaction (PCR) sequencing of the 16S rRNA gene confirmation. The hemolysis test and PCR were applied to detect the presence of virulence genes, namely toxR, thermostable direct haemolysin (tdh), and tdh-related haemolysin (trh). The Kirby-Bauer method was used for characterizing the resistance patterns against ampicillin (AMP), tetracycline (TET), cyprofloxacin (CIP), enrofloxacin (ENR), and chloramphenicol (CHL). The biochemical tests and PCR-16SrRNA gene sequencing confirmed that 12 isolates belonged to V. parahaemolyticus that were further verified by amplification of the toxR gene in 382 bp (100% of the isolates). The alpha hemolysis activity was also confirmed by the amplicon of 199 bp in all isolates. All V. parahaemolyticus isolates showed their resistance to AMP and 42% of the isolates were TET-resistant. However, no resistance was shown to CIP, ENR, and CHL. The PCR-based analysis resulted a detectable resistance gene of ampC (42% of the isolates) and tetB (83% of the isolates). \u0000Keywords: antibiotics, shrimp, resistance, virulency, Vibrio parahaemolyticus \u0000 \u0000ABSTRAK \u0000 \u0000Penelitian ini bertujuan untuk melakukan karakterisasi dan deteksi molekular dari gen patogenisitas dan resistansi antibiotik pada Vibrio parahaemolyticus, agen penyebab vibriosis pada udang vaname. Isolat V. parahaemolyticus dikoleksi dari hepatopankreas, diuji secara biokimiawi dan selanjutnya dikonfirmasi dengan polymerase chain reaction (PCR)-sekuensing dari gen 16S rRNA. Tes hemolisis dan metode PCR diterapkan untuk mendeteksi keberadaan gen virulensi toxR, thermostable direct haemolysin (tdh) and tdh-related haemolysin (trh). Metode Kirby Bauer digunakan untuk karakterisasi pola resistansi terhadap ampisilin (AMP), tetrasiklin (TET), kloramfenikol (CHL), siprofloksasin (CIP) dan enrofloksasin (ENR). Uji biokimia dan sekuensing gen PCR-16SrRNA memastikan bahwa 12 isolat adalah V. parahaemolyticus yang selanjutnya diverifikasi dengan amplifikasi gen toxR berukuran 382 bp (100% isolat). Aktivitas alfa hemolisis juga dikonfirmasi dengan amplikon PCR (199 bp) di semua isolat. Seluruh isolat V. parahaemolyticus menunjukkan resistansinya terhadap AMP, 42% resistan TET, tidak ada resistansi yang ditunjukkan pada CIP, ENR dan CHL. Analisis berbasis PCR menghasilkan gen resistan yang terdeteksi dari gen ampC (42% isolat) dan gen tetB (83% isolat). \u0000Kata kunci: Antibiotik, udang, resistansi, virulensi, Vibrio parahaemolyticus","PeriodicalId":32090,"journal":{"name":"Jurnal Akuakultur Indonesia","volume":"44 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85240659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：