Pub Date : 2021-04-03DOI: 10.1080/10496475.2021.1891173
S. E. El Sawi, A. Sleem, Rana M. Merghany
ABSTRACT The in vivo antioxidant, anti-inflammatory, and anti-hyperglycemic activities of Salvia splendens extracts (100 mg kg−1d−1 P.O.) in male albino rats were studied. The total 70% methanolic, total aqueous and successive ethyl acetate extracts demonstrated antioxidant and anti-inflammatory activities. The results suggested that S. splendens may have supportive role in controlling diabetic conditions due to the presence of poly-phenolic compounds.
{"title":"In Vivo Antioxidant, anti-Inflammatory and anti-Hyperglycemic Effects of Salvia Splendens Aerial Parts in Male Albino Rat Models","authors":"S. E. El Sawi, A. Sleem, Rana M. Merghany","doi":"10.1080/10496475.2021.1891173","DOIUrl":"https://doi.org/10.1080/10496475.2021.1891173","url":null,"abstract":"ABSTRACT The in vivo antioxidant, anti-inflammatory, and anti-hyperglycemic activities of Salvia splendens extracts (100 mg kg−1d−1 P.O.) in male albino rats were studied. The total 70% methanolic, total aqueous and successive ethyl acetate extracts demonstrated antioxidant and anti-inflammatory activities. The results suggested that S. splendens may have supportive role in controlling diabetic conditions due to the presence of poly-phenolic compounds.","PeriodicalId":35803,"journal":{"name":"Journal of Herbs, Spices and Medicinal Plants","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/10496475.2021.1891173","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49454356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-04-03DOI: 10.1080/10496475.2021.1891178
N. Malge, A.M.P.W. Bandara, W. Keerthirathna, D. Dissanayake, Patharage Kamal Perera, C. Witharana, L. Peiris
ABSTRACT The antioxidant, cytotoxic, and anti-cancer potential of aqueous root and rhizome extract (ARRE) of Smilax zeylanica was investigated against human liver carcinoma cells. The phytochemical composition was evaluated by standard methods, and confirmed by thin-layer chromatography. Free radical scavenging activity was determined by DPPH assay, cytotoxic activity by brine shrimp lethality assay, and anti-cancer potential was investigated using liver carcinoma HepG2 cells. Phytochemical analysis revealed the presence of saponins, flavonoids, proanthocyanins, tannins, and polyphenols. The total phenolic and flavonoid content varied from 0.28 to 5.26 mg gallic acid equivalent 100 mg−1 and 0.25–50.26 quercetin equivalent 100 mg−1, respectively. There was a linear relationship between the free radical scavenging activity and phytochemical concentrations with an IC50 value of 108 ± 1.2 µg mL−1 compared to the standard (IC50: 2.59 ± 0.02 µg mL−1). The brine shrimp lethality assay showed strong cell killing ability (IC50: 84 ± 0.7 µg mL−1) and the extract induced apoptosis of HepG2 cells (77 ± 1.52%) at 300 µg mL−1, comparable to the positive control (camptothecin).
{"title":"Antioxidant and Anti-proliferative Activities of Smilax Zeylanica Root and Rhizome Extract against Liver Carcinoma Cells","authors":"N. Malge, A.M.P.W. Bandara, W. Keerthirathna, D. Dissanayake, Patharage Kamal Perera, C. Witharana, L. Peiris","doi":"10.1080/10496475.2021.1891178","DOIUrl":"https://doi.org/10.1080/10496475.2021.1891178","url":null,"abstract":"ABSTRACT The antioxidant, cytotoxic, and anti-cancer potential of aqueous root and rhizome extract (ARRE) of Smilax zeylanica was investigated against human liver carcinoma cells. The phytochemical composition was evaluated by standard methods, and confirmed by thin-layer chromatography. Free radical scavenging activity was determined by DPPH assay, cytotoxic activity by brine shrimp lethality assay, and anti-cancer potential was investigated using liver carcinoma HepG2 cells. Phytochemical analysis revealed the presence of saponins, flavonoids, proanthocyanins, tannins, and polyphenols. The total phenolic and flavonoid content varied from 0.28 to 5.26 mg gallic acid equivalent 100 mg−1 and 0.25–50.26 quercetin equivalent 100 mg−1, respectively. There was a linear relationship between the free radical scavenging activity and phytochemical concentrations with an IC50 value of 108 ± 1.2 µg mL−1 compared to the standard (IC50: 2.59 ± 0.02 µg mL−1). The brine shrimp lethality assay showed strong cell killing ability (IC50: 84 ± 0.7 µg mL−1) and the extract induced apoptosis of HepG2 cells (77 ± 1.52%) at 300 µg mL−1, comparable to the positive control (camptothecin).","PeriodicalId":35803,"journal":{"name":"Journal of Herbs, Spices and Medicinal Plants","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/10496475.2021.1891178","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46976574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-04-03DOI: 10.1080/10496475.2021.1891175
Selma Silabdi, M. Khali, G. Tenore, P. Stiuso, D. Vanacore, E. Novellino
ABSTRACT The anti-hyperlipidemic potential of Algerian date palm fruits of three varieties [Deglet nour (DN), Ghars (GH), Degla baida (DB)] and its influence on oxidative stress were explored in rat models using human hepatocellular carcinoma cells (HepG2). Phenolic profile and total phenolic content of the extracts revealed in vitro antioxidant potential, which was confirmed using HepG2 cells. Reduction of TBARS level and increase in NO levels were observed in cell medium. In vivo assays showed that administration of date extracts influenced the hypolipidemic effect in rats by decreasing total cholesterol (−51% for DB, −31% for GH and −30% for DN), triacylglycerol (−61% for DN, −54% for DB and −32% for GH) and LDL-Cholesterol, while increasing HDL-Cholesterol level, indicating strong cardioprotective properties of date fruit.
{"title":"Phoenix dactylifera polyphenols improve plasma lipid profile in hyperlipidemic rats and oxidative stress on HepG2 cells","authors":"Selma Silabdi, M. Khali, G. Tenore, P. Stiuso, D. Vanacore, E. Novellino","doi":"10.1080/10496475.2021.1891175","DOIUrl":"https://doi.org/10.1080/10496475.2021.1891175","url":null,"abstract":"ABSTRACT The anti-hyperlipidemic potential of Algerian date palm fruits of three varieties [Deglet nour (DN), Ghars (GH), Degla baida (DB)] and its influence on oxidative stress were explored in rat models using human hepatocellular carcinoma cells (HepG2). Phenolic profile and total phenolic content of the extracts revealed in vitro antioxidant potential, which was confirmed using HepG2 cells. Reduction of TBARS level and increase in NO levels were observed in cell medium. In vivo assays showed that administration of date extracts influenced the hypolipidemic effect in rats by decreasing total cholesterol (−51% for DB, −31% for GH and −30% for DN), triacylglycerol (−61% for DN, −54% for DB and −32% for GH) and LDL-Cholesterol, while increasing HDL-Cholesterol level, indicating strong cardioprotective properties of date fruit.","PeriodicalId":35803,"journal":{"name":"Journal of Herbs, Spices and Medicinal Plants","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/10496475.2021.1891175","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45346373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-04-03DOI: 10.1080/10496475.2021.1891177
Liton Sikder, Mirxat Alim, Bushra Saieda, M. T. Islam, M. Martorell, J. Sharifi‐Rad, S. Khan
ABSTRACT The phytochemical composition of the ethanolic extract of Crinum viviparum was determined and the anti-bacterial, anti-inflammatory, thrombolytic, and anti-diarrheal activities were investigated by applying disc diffusion, egg albumin (in vitro), clot lysis, and castor oil-induced diarrhea model in mice (in vivo), respectively. The results suggested that the crude ethanolic extract of the plant contained alkaloids, tannins, flavonoids, steroids, and terpenoids. The extract was anti-bacterial against Escherichia coli, Bacillus subtilis, Staphylococcus aureus and Salmonella enterica. The plant showed anti-inflammatory and thrombolytic activities in a concentration-dependent manner. C. viviparum extract was anti-diarrheal in Swiss albino mice at 250 and 500 mg kg−1.
{"title":"Pharmacological Activities of Crinum viviparum: A Laboratory-based Study","authors":"Liton Sikder, Mirxat Alim, Bushra Saieda, M. T. Islam, M. Martorell, J. Sharifi‐Rad, S. Khan","doi":"10.1080/10496475.2021.1891177","DOIUrl":"https://doi.org/10.1080/10496475.2021.1891177","url":null,"abstract":"ABSTRACT The phytochemical composition of the ethanolic extract of Crinum viviparum was determined and the anti-bacterial, anti-inflammatory, thrombolytic, and anti-diarrheal activities were investigated by applying disc diffusion, egg albumin (in vitro), clot lysis, and castor oil-induced diarrhea model in mice (in vivo), respectively. The results suggested that the crude ethanolic extract of the plant contained alkaloids, tannins, flavonoids, steroids, and terpenoids. The extract was anti-bacterial against Escherichia coli, Bacillus subtilis, Staphylococcus aureus and Salmonella enterica. The plant showed anti-inflammatory and thrombolytic activities in a concentration-dependent manner. C. viviparum extract was anti-diarrheal in Swiss albino mice at 250 and 500 mg kg−1.","PeriodicalId":35803,"journal":{"name":"Journal of Herbs, Spices and Medicinal Plants","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/10496475.2021.1891177","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46714229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-04-03DOI: 10.1080/10496475.2021.1891179
S. Loganathan, K. Selvam, Vairakkannu Sivasakthi, P. Prakash, Myilswamy Yamuna, K. Lalitha, M. Shivakumar, S. Senthil Nathan
ABSTRACT Knoxia sumatrensis leaf extracts in methanol were analyzed for the phytochemical compositions, antioxidant properties (DPPH, ABTS, and hydroxyl radical assay), anti-proliferative activity (lung cancer A549 and breast cancer MCF-7 cell lines), and mosquito larvicidal properties. The GC-MS analysis showed the presence of eight bioactive compounds of which the hexadecanoic Acid, 2-Propyl-, methyl Ester, and 2,3-anhydro-D-Mannosan were the two major compounds present. The bioactive compounds present included phenolics and flavonoids (55.0 ± 1.3 mg GAE g−1 d.w. and 42.5 ± 0.5 mg RE g−1 d.w.). FT-IR analysis detected the presence of aldehydes and anhydrites functional groups. Antioxidant properties were positively associated with the secondary metabolites. The anti-proliferative activity of methanolic leaf extracts showed the IC50 values 135.82 and 181.73 μg mL−1 for A549 and MCF-7, respectively. The methanolic extract also showed larvicidal activity on three mosquito species with highest toxicity on Culex quinquefasciatus larvae with LC50 4.84 mg mL−1 and LC90 19.33 mg mL−1.
{"title":"Phytochemical and Pharmacological Evaluation of Methanolic Extract of Knoxia sumatrensis Leaves","authors":"S. Loganathan, K. Selvam, Vairakkannu Sivasakthi, P. Prakash, Myilswamy Yamuna, K. Lalitha, M. Shivakumar, S. Senthil Nathan","doi":"10.1080/10496475.2021.1891179","DOIUrl":"https://doi.org/10.1080/10496475.2021.1891179","url":null,"abstract":"ABSTRACT Knoxia sumatrensis leaf extracts in methanol were analyzed for the phytochemical compositions, antioxidant properties (DPPH, ABTS, and hydroxyl radical assay), anti-proliferative activity (lung cancer A549 and breast cancer MCF-7 cell lines), and mosquito larvicidal properties. The GC-MS analysis showed the presence of eight bioactive compounds of which the hexadecanoic Acid, 2-Propyl-, methyl Ester, and 2,3-anhydro-D-Mannosan were the two major compounds present. The bioactive compounds present included phenolics and flavonoids (55.0 ± 1.3 mg GAE g−1 d.w. and 42.5 ± 0.5 mg RE g−1 d.w.). FT-IR analysis detected the presence of aldehydes and anhydrites functional groups. Antioxidant properties were positively associated with the secondary metabolites. The anti-proliferative activity of methanolic leaf extracts showed the IC50 values 135.82 and 181.73 μg mL−1 for A549 and MCF-7, respectively. The methanolic extract also showed larvicidal activity on three mosquito species with highest toxicity on Culex quinquefasciatus larvae with LC50 4.84 mg mL−1 and LC90 19.33 mg mL−1.","PeriodicalId":35803,"journal":{"name":"Journal of Herbs, Spices and Medicinal Plants","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/10496475.2021.1891179","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47221570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-03-12DOI: 10.1080/10496475.2021.1891174
Mouna Mokhtari, S. Chabani, Soumia Mouffouk, M. Aberkane, A. Dibi, M. Benkhaled, Hamada Haba
ABSTRACT The chemical composition and anti-inflammatory, antihemolytic, antioxidant, and antibacterial activities of solvent extracts of Thymus algeriensis in petroleum ether, chloroform, and n-butanol were evaluated. Ten compounds including four flavonoids, four phenolics, and two triterpenoids were isolated and identified by chromatography and spectroscopy. The n-butanol extract had the greatest antioxidant activity and inhibited hemolysis with a value of IC50 at 322.85 ± 0.87 µg mL−1. The chloroform extract exhibited the highest anti-inflammatory effect with 45.27% inhibition. The extracts demonstrated antibacterial activity against the tested strains.
{"title":"Phytochemicals, Antihemolytic, Anti-inflammatory, Antioxidant, and Antibacterial Activities from Thymus Algeriensis","authors":"Mouna Mokhtari, S. Chabani, Soumia Mouffouk, M. Aberkane, A. Dibi, M. Benkhaled, Hamada Haba","doi":"10.1080/10496475.2021.1891174","DOIUrl":"https://doi.org/10.1080/10496475.2021.1891174","url":null,"abstract":"ABSTRACT The chemical composition and anti-inflammatory, antihemolytic, antioxidant, and antibacterial activities of solvent extracts of Thymus algeriensis in petroleum ether, chloroform, and n-butanol were evaluated. Ten compounds including four flavonoids, four phenolics, and two triterpenoids were isolated and identified by chromatography and spectroscopy. The n-butanol extract had the greatest antioxidant activity and inhibited hemolysis with a value of IC50 at 322.85 ± 0.87 µg mL−1. The chloroform extract exhibited the highest anti-inflammatory effect with 45.27% inhibition. The extracts demonstrated antibacterial activity against the tested strains.","PeriodicalId":35803,"journal":{"name":"Journal of Herbs, Spices and Medicinal Plants","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/10496475.2021.1891174","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41766691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-03-09DOI: 10.1080/10496475.2021.1891181
Chilivery Ravi Kiran, C. Rajkuberan, A. Sangilimuthu, F. Hakkim, H. Bakshi, Sowmya Priya Manoharan
ABSTRACT The calcium oxalate reduction capacity of Argemone mexicana was demonstrated in vivo in ethylene glycol-induced urolithic rats. A. mexicana leaves extract at 400 and 750 mg kg−1 b.w. demonstrated protection of renal tubes, kidney cells and reduced the levels of serum creatinine and calcium compared to the urolithiasis induced group. Further, A. mexicana restored blood and urine markers to normal before and after treatment in urolithiasis rats and caused the breakdown of calcium oxalate crystals in the urinary tract as observed by microscopy. The A. mexicana extract protected from renal damage and repaired and increased diuresis activity thereby completely excreted calcium oxalate crystals in renal tubes. Analysis of methanolic extract of A. mexicana showed the presence of a 3,4-dihydro-3-hydroxy-7-(7-methyloctyl)naphthalen-2(1 H)-one as the bioactive compound which may be attributed to the antiurolithiatic activity.
摘要:在乙二醇诱导的尿结石大鼠体内，研究了银银酮还原草酸钙的能力。与尿石症诱导组相比，400和750 mg kg - 1 b.w.的墨西哥牛蒡叶提取物显示出对肾管、肾细胞的保护作用，并降低了血清肌酐和钙的水平。此外，在显微镜下观察到，墨西哥刺槐使尿石症大鼠治疗前后的血液和尿液指标恢复正常，并引起尿路草酸钙晶体的分解。墨西哥菊提取物保护肾脏免受损害，修复和增加利尿活性，从而完全排出肾管中的草酸钙晶体。对墨西哥香甲醇提取物进行分析，发现其主要活性物质为3,4-二氢-3-羟基-7-(7-甲基新基)萘-2(1 H)- 1，其抗尿石活性可能与此有关。
{"title":"Intrinsic Evaluation of Antiurolithiatic Capacity of Argemone mexicana L. in Wistar Albino Rats","authors":"Chilivery Ravi Kiran, C. Rajkuberan, A. Sangilimuthu, F. Hakkim, H. Bakshi, Sowmya Priya Manoharan","doi":"10.1080/10496475.2021.1891181","DOIUrl":"https://doi.org/10.1080/10496475.2021.1891181","url":null,"abstract":"ABSTRACT The calcium oxalate reduction capacity of Argemone mexicana was demonstrated in vivo in ethylene glycol-induced urolithic rats. A. mexicana leaves extract at 400 and 750 mg kg−1 b.w. demonstrated protection of renal tubes, kidney cells and reduced the levels of serum creatinine and calcium compared to the urolithiasis induced group. Further, A. mexicana restored blood and urine markers to normal before and after treatment in urolithiasis rats and caused the breakdown of calcium oxalate crystals in the urinary tract as observed by microscopy. The A. mexicana extract protected from renal damage and repaired and increased diuresis activity thereby completely excreted calcium oxalate crystals in renal tubes. Analysis of methanolic extract of A. mexicana showed the presence of a 3,4-dihydro-3-hydroxy-7-(7-methyloctyl)naphthalen-2(1 H)-one as the bioactive compound which may be attributed to the antiurolithiatic activity.","PeriodicalId":35803,"journal":{"name":"Journal of Herbs, Spices and Medicinal Plants","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/10496475.2021.1891181","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41937681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-03-02DOI: 10.1080/10496475.2021.1891182
O. Enechi, I. Okagu, C. Ezumezu
ABSTRACT This study evaluated the effects of methanol extract of Peltophorum pterocarpum stem bark (MEPTS) on blood glucose level and lipid profile of Plasmodium berghei ANKA 65-parasitized mice. The levels of total cholesterol (TC), low- and high-density lipoproteins (LDL and HDL, respectively), triacylglycerols (TAG) and blood glucose in parasitized mice treated with MEPTS (100, 200 or 400 mg kg−1 b.w. d−1, p.o) and artemether/lumenfantrin (28 mg kg−1 b.w. d−1, p.o) were determined and compared with malaria control. Alkaloids, saponins, flavonoids, tannins, glycosides, steroids, carotenoids, anthocyanins, anthraquinones, terpenoids, and phenols were detected in the extract. On days 1, 2, and 3, there were progressive increase in malaria parasitemia in malaria control compared with parasitized-MEPTS-administered mice that had dose-dependent decrease in malaria parasitemia. Malaria control group showed hyperlipidemia and hypoglycemia compared to normal mice, which was ameliorated by the administration of MEPTS, and artemether/lumenfantrin. These findings suggest that MEPTS may have potent antimalarial activity and modulated lipid dysfunction and hypoglycemia generated by malaria.
摘要本研究评价了紫杉茎皮甲醇提取物(MEPTS)对伯氏疟原虫ANKA 65感染小鼠血糖水平和血脂的影响。测定经MEPTS(100、200或400 mg kg - 1 b.w. d - 1, p.o)和蒿甲醚/lumenfantrin (28 mg kg - 1 b.w. d - 1, p.o)处理的寄生小鼠的总胆固醇(TC)、低脂蛋白和高密度脂蛋白(LDL和HDL)、三酰甘油(TAG)和血糖水平，并与疟疾对照进行比较。提取液中检出生物碱、皂苷、黄酮类、单宁、糖苷、类固醇、类胡萝卜素、花青素、蒽醌、萜类和酚类。在第1、2和3天，疟疾控制组的疟疾寄生虫率逐渐增加，而给药mepts的小鼠疟疾寄生虫率呈剂量依赖性下降。与正常小鼠相比，疟疾对照组出现高脂血症和低血糖症，给予MEPTS和蒿甲醚/流明芬肽可改善这一情况。这些发现表明，MEPTS可能具有有效的抗疟疾活性，并可调节疟疾引起的脂质功能障碍和低血糖。
{"title":"Methanol extract of Peltophorum Pterocarpum stem bark modulates Plasmodium berghei ANKA 65-Induced hypoglycemia and lipid dysfunction in Mice","authors":"O. Enechi, I. Okagu, C. Ezumezu","doi":"10.1080/10496475.2021.1891182","DOIUrl":"https://doi.org/10.1080/10496475.2021.1891182","url":null,"abstract":"ABSTRACT This study evaluated the effects of methanol extract of Peltophorum pterocarpum stem bark (MEPTS) on blood glucose level and lipid profile of Plasmodium berghei ANKA 65-parasitized mice. The levels of total cholesterol (TC), low- and high-density lipoproteins (LDL and HDL, respectively), triacylglycerols (TAG) and blood glucose in parasitized mice treated with MEPTS (100, 200 or 400 mg kg−1 b.w. d−1, p.o) and artemether/lumenfantrin (28 mg kg−1 b.w. d−1, p.o) were determined and compared with malaria control. Alkaloids, saponins, flavonoids, tannins, glycosides, steroids, carotenoids, anthocyanins, anthraquinones, terpenoids, and phenols were detected in the extract. On days 1, 2, and 3, there were progressive increase in malaria parasitemia in malaria control compared with parasitized-MEPTS-administered mice that had dose-dependent decrease in malaria parasitemia. Malaria control group showed hyperlipidemia and hypoglycemia compared to normal mice, which was ameliorated by the administration of MEPTS, and artemether/lumenfantrin. These findings suggest that MEPTS may have potent antimalarial activity and modulated lipid dysfunction and hypoglycemia generated by malaria.","PeriodicalId":35803,"journal":{"name":"Journal of Herbs, Spices and Medicinal Plants","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/10496475.2021.1891182","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46868933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-03-01DOI: 10.1080/10496475.2021.1891176
H. Mohammed, A. Sallau, Humphrey Chukwu Nzelibe, A. Muhammad, O. Erukainure, B. Musa, M. K. Anigo
ABSTRACT The antihyperlipidemic and antioxidant effects of Mentha piperita leaves extracts in Poloxamer 407-induced hyperlipidemic rats were investigated. Antihyperlipidemic activities of hexane, ethyl acetate, and methanol extracts (100, 200, or 300 mg kg−1 b.w.) were evaluated by lipid profile analysis. Twelve (12) fractions (F1 to F12) were collected from the most antihyperlipidemic extract (ethyl acetate at 100 mg kg−1) and, assayed in vivo for antihyperlipidemic activity. The effects of the most antihyperlipidemic fraction (F2) on redox sensitive biomarkers (SOD, CAT, GSH, MDA) and lipoprotein lipase (LPL) activity at cellular and molecular levels were evaluated. Fraction-2 (F2) was further subjected to GC-MS and LC-MS analysis for identification of nonpolar and polar components. The F2 treatment decreased the level of MDA with concomitant enhancement of GSH, LPL activity in the liver and serum and up regulated the expression of SOD, CAT, and LPL genes in liver and blood relative to the untreated rats. GC-MS and LC-MS revealed oleic acid, vaccenic acid, 3, 4-dicaffeoylquinic acid, coumarin, and triacontane as the probable bioactive components with reported antihyperlipidemic and antioxidant effects.
摘要:研究薄荷叶提取物对poloxam407诱导的高脂血症大鼠的降血脂和抗氧化作用。通过脂质谱分析评估己烷、乙酸乙酯和甲醇提取物(100、200或300 mg kg - 1 b.w)的降血脂活性。从最有效的抗高血脂提取物(乙酸乙酯浓度为100 mg kg - 1)中收集12 (F1至F12)个部分，并在体内测定其抗高血脂活性。在细胞和分子水平上评估了抗高脂血症部分(F2)对氧化还原敏感生物标志物(SOD、CAT、GSH、MDA)和脂蛋白脂肪酶(LPL)活性的影响。部分-2 (F2)进一步进行GC-MS和LC-MS分析，以鉴定非极性和极性成分。与未处理大鼠相比，F2处理降低了MDA水平，同时提高了肝脏和血清中GSH、LPL活性，上调了肝脏和血液中SOD、CAT和LPL基因的表达。GC-MS和LC-MS显示油酸、异丙酸、3,4 -二咖啡酰奎宁酸、香豆素和三康烷可能是具有抗高脂血症和抗氧化作用的生物活性成分。
{"title":"Bioactive Fraction of Mentha piperita Leaves Extract Stimulates Lipoprotein Lipase at Cellular and Molecular Levels via Augmentation of Antioxidant Defense System in Hyperlipidemic Rats","authors":"H. Mohammed, A. Sallau, Humphrey Chukwu Nzelibe, A. Muhammad, O. Erukainure, B. Musa, M. K. Anigo","doi":"10.1080/10496475.2021.1891176","DOIUrl":"https://doi.org/10.1080/10496475.2021.1891176","url":null,"abstract":"ABSTRACT The antihyperlipidemic and antioxidant effects of Mentha piperita leaves extracts in Poloxamer 407-induced hyperlipidemic rats were investigated. Antihyperlipidemic activities of hexane, ethyl acetate, and methanol extracts (100, 200, or 300 mg kg−1 b.w.) were evaluated by lipid profile analysis. Twelve (12) fractions (F1 to F12) were collected from the most antihyperlipidemic extract (ethyl acetate at 100 mg kg−1) and, assayed in vivo for antihyperlipidemic activity. The effects of the most antihyperlipidemic fraction (F2) on redox sensitive biomarkers (SOD, CAT, GSH, MDA) and lipoprotein lipase (LPL) activity at cellular and molecular levels were evaluated. Fraction-2 (F2) was further subjected to GC-MS and LC-MS analysis for identification of nonpolar and polar components. The F2 treatment decreased the level of MDA with concomitant enhancement of GSH, LPL activity in the liver and serum and up regulated the expression of SOD, CAT, and LPL genes in liver and blood relative to the untreated rats. GC-MS and LC-MS revealed oleic acid, vaccenic acid, 3, 4-dicaffeoylquinic acid, coumarin, and triacontane as the probable bioactive components with reported antihyperlipidemic and antioxidant effects.","PeriodicalId":35803,"journal":{"name":"Journal of Herbs, Spices and Medicinal Plants","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/10496475.2021.1891176","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41417554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-02-26DOI: 10.1080/10496475.2021.1891180
K. Akinwunmi, Esther Odunsi, C. Amadi, Ezekiel G Ajayi
ABSTRACT The anti-inflammatory and antibacterial activity of the methanolic extract and fractions of Chrysanthemum procumetens leaves were evaluated. The extract was obtained from dried leaf samples using 70% methanol and screened for phytochemical constituents. The crude extract was partitioned successively with n-hexane, ethyl acetate and butanol. Membrane stability and inhibition of albumin denaturation assays were done to evaluate the in vitro anti-inflammatory activity. Antibacterial activity was determined using six test bacteria strains of both Gram negative and Gram positive bacteria. Phytochemical screening tested positive for flavonoids, alkaloids, saponins, steroids, tannins and cardiac glycosides. The crude extract had the highest total phenol (344.67 ± 11.26 mg GAE g−1) and flavonoids (230.30 ± 22.11 mg RE g−1) while the butanol fraction had the lowest total phenol and total flavonoids (117.57 ± 9.40 mg GAE g−1 and 100.60 ± 1.99 mg RE g−1, respectively). The crude extracts and fractions exhibited concentration-dependent anti-inflammatory activity, comparable to Ibuprofen. The anti-bacterial activity of the crude extract and ethyl acetate fraction of C. procumetens were positive for Pseudomonas aeruginosa with inhibition zone of 24.30 ± 0.02 mm; 15.5 ± 0.02 mm and MIC of 1.70 ± 0.01 mg mL−1 and 2.00 ± 0.03 mg mL−1, respectively.
{"title":"Anti-inflammatory and Antibacterial Potentials of the Leaf Extract and Fractions of Chrysanthemum procumetens","authors":"K. Akinwunmi, Esther Odunsi, C. Amadi, Ezekiel G Ajayi","doi":"10.1080/10496475.2021.1891180","DOIUrl":"https://doi.org/10.1080/10496475.2021.1891180","url":null,"abstract":"ABSTRACT The anti-inflammatory and antibacterial activity of the methanolic extract and fractions of Chrysanthemum procumetens leaves were evaluated. The extract was obtained from dried leaf samples using 70% methanol and screened for phytochemical constituents. The crude extract was partitioned successively with n-hexane, ethyl acetate and butanol. Membrane stability and inhibition of albumin denaturation assays were done to evaluate the in vitro anti-inflammatory activity. Antibacterial activity was determined using six test bacteria strains of both Gram negative and Gram positive bacteria. Phytochemical screening tested positive for flavonoids, alkaloids, saponins, steroids, tannins and cardiac glycosides. The crude extract had the highest total phenol (344.67 ± 11.26 mg GAE g−1) and flavonoids (230.30 ± 22.11 mg RE g−1) while the butanol fraction had the lowest total phenol and total flavonoids (117.57 ± 9.40 mg GAE g−1 and 100.60 ± 1.99 mg RE g−1, respectively). The crude extracts and fractions exhibited concentration-dependent anti-inflammatory activity, comparable to Ibuprofen. The anti-bacterial activity of the crude extract and ethyl acetate fraction of C. procumetens were positive for Pseudomonas aeruginosa with inhibition zone of 24.30 ± 0.02 mm; 15.5 ± 0.02 mm and MIC of 1.70 ± 0.01 mg mL−1 and 2.00 ± 0.03 mg mL−1, respectively.","PeriodicalId":35803,"journal":{"name":"Journal of Herbs, Spices and Medicinal Plants","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/10496475.2021.1891180","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46439568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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