Pub Date : 2022-02-13DOI: 10.1080/10496475.2022.2034701
Parismita Borgohain, P. Barua, Dhrubajyoti D. Das, J. Mahanta, B. Saikia, P. Dutta, L. R. Saikia
ABSTRACT This study evaluated the antifungal activity of the traditional medicinal plants used by agricultural workers to treat onychomycosis. Of all tested plants, methanol leaf extracts of Croton tiglium possessed antifungal activity against many isolates. Azulene, 9-Hexadecenoic acid, Octadecane, and 3-ethyl-5-(2-ethylbutyl) were the major antifungal compounds identified by GC-MS/MS. Broad ranges of antifungal properties of C. tiglium makes it a potential candidate for the treatment of onychomycosis without health hazard.
{"title":"Antifungal Activity of Selected Medicinal Plants Used by Indigenous People of Assam in India to Treat Onychomycosis","authors":"Parismita Borgohain, P. Barua, Dhrubajyoti D. Das, J. Mahanta, B. Saikia, P. Dutta, L. R. Saikia","doi":"10.1080/10496475.2022.2034701","DOIUrl":"https://doi.org/10.1080/10496475.2022.2034701","url":null,"abstract":"ABSTRACT This study evaluated the antifungal activity of the traditional medicinal plants used by agricultural workers to treat onychomycosis. Of all tested plants, methanol leaf extracts of Croton tiglium possessed antifungal activity against many isolates. Azulene, 9-Hexadecenoic acid, Octadecane, and 3-ethyl-5-(2-ethylbutyl) were the major antifungal compounds identified by GC-MS/MS. Broad ranges of antifungal properties of C. tiglium makes it a potential candidate for the treatment of onychomycosis without health hazard.","PeriodicalId":35803,"journal":{"name":"Journal of Herbs, Spices and Medicinal Plants","volume":"28 1","pages":"160 - 178"},"PeriodicalIF":0.0,"publicationDate":"2022-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44565229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-12-28DOI: 10.1080/10496475.2021.2015506
S. Paul, K. Naher, Sanzida Yeasmin, Rabindra Nath Acharrya, M. Golder, K. S. Ahmed, Hemayet Hossain, Shrabanti Dev, A. Das
ABSTRACT Ethanolic leaf extract of Tamarix indica was evaluated for the antioxidant and anticancer activities and toxicological profiles. The flavonoid, phenolic, and tannin contents of leaves were estimated as 5.3068 mg of QE g−1, 8.786 mg of GAE g−1, and 8.5216 mg of GAE g−1, respectively. In DPPH scavenging assay, T. indica leaves’ extract had an IC50 value of 74.38 μg mL−1. HPLC-DAD analysis identified catechin hydrate, vanillic acid, rutin hydrate, p-coumaric acid, and myricetin in different concentrations. In brine shrimp lethality bioassay, the IC50 value of the extract was 19.928 µg mL−1. Chemopreventive activity was evaluated using DMBA/croton oil-induced skin cancinogenic model mice. Oral treatment of the extract at different doses reduced the size, number, yield, and burden of tumor in skin carcinogenic mice. Natural antioxidants GSH and SOD increased with extract treatment. Additionally, reduction in blood bilirubin, SGPT, and SGOT levels were observed in extract treated mice. In acute toxicity study, no mortality was observed in mice justifying the safety of the extract.
{"title":"Phytochemicals, Antioxidant, and Chemopreventive Potential of Tamarix Indica Leaf Extracts","authors":"S. Paul, K. Naher, Sanzida Yeasmin, Rabindra Nath Acharrya, M. Golder, K. S. Ahmed, Hemayet Hossain, Shrabanti Dev, A. Das","doi":"10.1080/10496475.2021.2015506","DOIUrl":"https://doi.org/10.1080/10496475.2021.2015506","url":null,"abstract":"ABSTRACT Ethanolic leaf extract of Tamarix indica was evaluated for the antioxidant and anticancer activities and toxicological profiles. The flavonoid, phenolic, and tannin contents of leaves were estimated as 5.3068 mg of QE g−1, 8.786 mg of GAE g−1, and 8.5216 mg of GAE g−1, respectively. In DPPH scavenging assay, T. indica leaves’ extract had an IC50 value of 74.38 μg mL−1. HPLC-DAD analysis identified catechin hydrate, vanillic acid, rutin hydrate, p-coumaric acid, and myricetin in different concentrations. In brine shrimp lethality bioassay, the IC50 value of the extract was 19.928 µg mL−1. Chemopreventive activity was evaluated using DMBA/croton oil-induced skin cancinogenic model mice. Oral treatment of the extract at different doses reduced the size, number, yield, and burden of tumor in skin carcinogenic mice. Natural antioxidants GSH and SOD increased with extract treatment. Additionally, reduction in blood bilirubin, SGPT, and SGOT levels were observed in extract treated mice. In acute toxicity study, no mortality was observed in mice justifying the safety of the extract.","PeriodicalId":35803,"journal":{"name":"Journal of Herbs, Spices and Medicinal Plants","volume":"28 1","pages":"143 - 159"},"PeriodicalIF":0.0,"publicationDate":"2021-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47277957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-12-11DOI: 10.1080/10496475.2021.2014012
T. A. Mir, Muatasim Jan, R. Khare
ABSTRACT The indigenous knowledge and conservation status of medicinal plants were explored in the Bandipora district of the Kashmir Himalaya, Jammu and Kashmir, using semi-structured interviews and group discussions. A total of 71 plant species belonging to 43 families, with the predominance of Asteraceae and Lamiaceae were used in traditional medicine by the tribal communities in the area. Local communities used these plants for treating constipation, digestive disorders, fever, diarrhea, joint pain and as tonic. Medicinal use of plant species in various families was similar to previous reports from Kashmir Himalaya. Artemisia absinthium, Cichorium intybus, Foeniculum vulgare, Taraxacum campylodes and Mentha arvensis were frequently used due to their abundance in the study area.
{"title":"Ethnomedicinal Practices and Conservation Status of Medicinal Plants in the Bandipora District of Kashmir Himalaya","authors":"T. A. Mir, Muatasim Jan, R. Khare","doi":"10.1080/10496475.2021.2014012","DOIUrl":"https://doi.org/10.1080/10496475.2021.2014012","url":null,"abstract":"ABSTRACT The indigenous knowledge and conservation status of medicinal plants were explored in the Bandipora district of the Kashmir Himalaya, Jammu and Kashmir, using semi-structured interviews and group discussions. A total of 71 plant species belonging to 43 families, with the predominance of Asteraceae and Lamiaceae were used in traditional medicine by the tribal communities in the area. Local communities used these plants for treating constipation, digestive disorders, fever, diarrhea, joint pain and as tonic. Medicinal use of plant species in various families was similar to previous reports from Kashmir Himalaya. Artemisia absinthium, Cichorium intybus, Foeniculum vulgare, Taraxacum campylodes and Mentha arvensis were frequently used due to their abundance in the study area.","PeriodicalId":35803,"journal":{"name":"Journal of Herbs, Spices and Medicinal Plants","volume":"28 1","pages":"125 - 142"},"PeriodicalIF":0.0,"publicationDate":"2021-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45070070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-12-01DOI: 10.1080/10496475.2021.2008085
Aikaterina L. Stefi, N. Christodoulakis
ABSTRACT An integrated microscopical investigation of the secretive activity in the above ground parts such as the leaves, petioles, and young stems of the camphor tree (Cinnamomum camphora) was performed. Different kinds of cells were localized accumulating different metabolites as this was demonstrated by histochemical tests. The wide distribution of oil and mucilage cells and other idioblasts was set out. Virtually all palisade cells appeared to be involved in secondary metabolite production which may explain the complexity of the plant extracts and their wide range of therapeutic anti-inflammatory and anti-oxidative effects they have in traditional medicine.
{"title":"Leaf Structure and Histochemical Analysis of Camphor Tree (Cinnamomum Camphora), a Plant Used in Traditional Remedies","authors":"Aikaterina L. Stefi, N. Christodoulakis","doi":"10.1080/10496475.2021.2008085","DOIUrl":"https://doi.org/10.1080/10496475.2021.2008085","url":null,"abstract":"ABSTRACT An integrated microscopical investigation of the secretive activity in the above ground parts such as the leaves, petioles, and young stems of the camphor tree (Cinnamomum camphora) was performed. Different kinds of cells were localized accumulating different metabolites as this was demonstrated by histochemical tests. The wide distribution of oil and mucilage cells and other idioblasts was set out. Virtually all palisade cells appeared to be involved in secondary metabolite production which may explain the complexity of the plant extracts and their wide range of therapeutic anti-inflammatory and anti-oxidative effects they have in traditional medicine.","PeriodicalId":35803,"journal":{"name":"Journal of Herbs, Spices and Medicinal Plants","volume":"28 1","pages":"113 - 124"},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43149803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ABSTRACT Crude EtO1 (ethanol maceration) obtained from aerial portions of dill was divided and eluted by solvents, to obtain PEt (petroleum ether), Eth (ethyl acetate) and EtO2 (ethanol) fractions, evaluated for total phenolics and flavonoids and antioxidant (DPPH, ABTS and FRAP assay), anti-protein denaturation, and anti-enzymatic (tyrosinase and collagenase) activities. The PEt extract was separated by column chromatography, while the chemical composition and biological effects were examined by GC-MS. All the extracts contained phenolics and flavonoids and demonstrated antioxidant activities in all assays. PEt extracts showed anti-protein denaturation (IC50 40.92 µg mL−1). A potential of collagenase inhibitor (92.35–98.69%) similar to Epigallocatechin gallate (EGCG) was noted in all extracts. Five compounds (C3, C5A, C5B2b, C9-1 and C9C9 eluted from Pet extract) represented fatty acid and fatty alcohol (nonacosan-10-one, hexadecanoic acid, ethyl ester, octacosanol, 1-hexacosanol, and hexacosanol) as major components and demonstrated inhibition of protein-denaturation (22.85–42.35% except C9-1). The compounds C9C9 and C9-1 demonstrated collagenase inhibitor effect.
{"title":"Bioassay Guided-isolation of Phytochemicals and Their Biological Effects of Solvent Extracts of Dill (Anethum Graveolens) Aerial Portions","authors":"Nichakan Peerakam, Sanit Thongnest, Prapart Phoowiang, Kwanchayanawish Machana","doi":"10.1080/10496475.2021.1989734","DOIUrl":"https://doi.org/10.1080/10496475.2021.1989734","url":null,"abstract":"ABSTRACT Crude EtO1 (ethanol maceration) obtained from aerial portions of dill was divided and eluted by solvents, to obtain PEt (petroleum ether), Eth (ethyl acetate) and EtO2 (ethanol) fractions, evaluated for total phenolics and flavonoids and antioxidant (DPPH, ABTS and FRAP assay), anti-protein denaturation, and anti-enzymatic (tyrosinase and collagenase) activities. The PEt extract was separated by column chromatography, while the chemical composition and biological effects were examined by GC-MS. All the extracts contained phenolics and flavonoids and demonstrated antioxidant activities in all assays. PEt extracts showed anti-protein denaturation (IC50 40.92 µg mL−1). A potential of collagenase inhibitor (92.35–98.69%) similar to Epigallocatechin gallate (EGCG) was noted in all extracts. Five compounds (C3, C5A, C5B2b, C9-1 and C9C9 eluted from Pet extract) represented fatty acid and fatty alcohol (nonacosan-10-one, hexadecanoic acid, ethyl ester, octacosanol, 1-hexacosanol, and hexacosanol) as major components and demonstrated inhibition of protein-denaturation (22.85–42.35% except C9-1). The compounds C9C9 and C9-1 demonstrated collagenase inhibitor effect.","PeriodicalId":35803,"journal":{"name":"Journal of Herbs, Spices and Medicinal Plants","volume":"28 1","pages":"99 - 111"},"PeriodicalIF":0.0,"publicationDate":"2021-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41368721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-10-02DOI: 10.1080/10496475.2021.1943591
A. Bhaskar, Shailasree Sekhar
ABSTRACT The methanol extract of Delonix regia bark (DMeOH) was investigated for its anti-inflammatory property in kappa-carrageenan (Carr)-induced hind paw edema of rats. DMeOH was orally administered and evaluated as a measure of edema, tissue destruction, and neutrophil migration to the inflammation site. The pro-inflammatory mediators, p-p65 subunit of NFκB, interleukin (IL)-1β, cycloxygenase (COX)-2 and inducible nitric oxide synthase (iNOS) were analyzed. Catalase (CAT) and superoxide dismutase (SOD) enzyme activity assays and malondialdehye (MDA) were quantified. The LC-MS fingerprint of the extract identified 10 polyphenols, of which five were structurally characterized based on their [M-H]− peak, UV-vis absorption compared to published data. Anti-inflammatory studies revealed that pre-treatment with DMeOH (0.5 g kg−1) prior to Carr-injection inhibited paw edema abating tissue destruction with decreased neutrophil infiltration. Carr-injection resulted in increased NO(•-) production, iNOS transcript and p-p65 subunit of NF-κB protein. DMeOH feed inhibited NO(•-) production and iNOS transcript levels. A decreased expression of NF-κB p-p65 subunit, COX-2 and IL-1β at 0.5 g kg−1 b.w oral feed suggest that DMeOH exerted its anti-inflammatory property by inhibiting NFκB activation. An increase in the antioxidant enzyme activities of CAT (78%) and SOD (49%) with reduced MDA levels was recorded.
摘要:本文研究了帝龙树皮甲醇提取物(DMeOH)对卡帕-卡拉胶(Carr)诱导的大鼠后足水肿的抗炎作用。口服DMeOH，并评估其水肿、组织破坏和中性粒细胞向炎症部位迁移的程度。分析促炎介质、NFκB的p-p65亚基、白细胞介素(IL)-1β、环氧化酶(COX)-2和诱导型一氧化氮合酶(iNOS)。测定过氧化氢酶(CAT)、超氧化物歧化酶(SOD)活性及丙二醛(MDA)含量。提取液的LC-MS指纹图谱鉴定出10种多酚，其中5种多酚的[M-H]−峰和紫外-可见吸收光谱与已发表的数据进行了结构表征。抗炎研究显示，在carr注射前用DMeOH (0.5 g kg - 1)预处理可抑制足跖水肿，减轻组织破坏，减少中性粒细胞浸润。carr注射导致NO(•-)生成、iNOS转录和NF-κ b蛋白p-p65亚基升高。DMeOH饲料抑制NO(•-)生成和iNOS转录水平。0.5 g kg -1 b.w口服饲料可降低NF-κB p-p65亚基、COX-2和IL-1β的表达，表明DMeOH通过抑制NF-κB活化发挥其抗炎作用。随着MDA水平的降低，CAT(78%)和SOD(49%)的抗氧化酶活性升高。
{"title":"Delonix Regia Bark Alleviated Inflammation by Stimulating Defense Responses in Rats","authors":"A. Bhaskar, Shailasree Sekhar","doi":"10.1080/10496475.2021.1943591","DOIUrl":"https://doi.org/10.1080/10496475.2021.1943591","url":null,"abstract":"ABSTRACT The methanol extract of Delonix regia bark (DMeOH) was investigated for its anti-inflammatory property in kappa-carrageenan (Carr)-induced hind paw edema of rats. DMeOH was orally administered and evaluated as a measure of edema, tissue destruction, and neutrophil migration to the inflammation site. The pro-inflammatory mediators, p-p65 subunit of NFκB, interleukin (IL)-1β, cycloxygenase (COX)-2 and inducible nitric oxide synthase (iNOS) were analyzed. Catalase (CAT) and superoxide dismutase (SOD) enzyme activity assays and malondialdehye (MDA) were quantified. The LC-MS fingerprint of the extract identified 10 polyphenols, of which five were structurally characterized based on their [M-H]− peak, UV-vis absorption compared to published data. Anti-inflammatory studies revealed that pre-treatment with DMeOH (0.5 g kg−1) prior to Carr-injection inhibited paw edema abating tissue destruction with decreased neutrophil infiltration. Carr-injection resulted in increased NO(•-) production, iNOS transcript and p-p65 subunit of NF-κB protein. DMeOH feed inhibited NO(•-) production and iNOS transcript levels. A decreased expression of NF-κB p-p65 subunit, COX-2 and IL-1β at 0.5 g kg−1 b.w oral feed suggest that DMeOH exerted its anti-inflammatory property by inhibiting NFκB activation. An increase in the antioxidant enzyme activities of CAT (78%) and SOD (49%) with reduced MDA levels was recorded.","PeriodicalId":35803,"journal":{"name":"Journal of Herbs, Spices and Medicinal Plants","volume":"27 1","pages":"411 - 425"},"PeriodicalIF":0.0,"publicationDate":"2021-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45761769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-10-02DOI: 10.1080/10496475.2021.1947928
I. Sellem, A. Chakchouk-Mtibaa, S. Smaoui, L. Mellouli
ABSTRACT The biological activities of Inula graveolens in Chebba (Tunisia) salt marsh were screened. Total polyphenol (TPP), flavonoid (TF), and proanthocyanidin (TPC) contents were determined and the correlation between the phytochemistry and the antioxidant, antimicrobial, and anti-enzymatic activities was assessed. The polyphenols in the solvent extracts suggested that salinity stress may enhance the production of bioactive compounds. Antiradical activities and a potent acetylcholinesterase inhibitory power were observed. I. graveolens extracts were antimicrobial against Salmonella typhimurium, Micrococcus luteus, and Staphylococcus aureus. Total flavonoids correlated with the activity against M. luteus, while the total polyphenols content was not correlated with the antimicrobial activity.
{"title":"Total Polyphenol, Flavonoid, and Proanthocyanidin Contents and Biological Activities of Inula Graveolens Collected from Chebba (Tunisia) Salt Marsh","authors":"I. Sellem, A. Chakchouk-Mtibaa, S. Smaoui, L. Mellouli","doi":"10.1080/10496475.2021.1947928","DOIUrl":"https://doi.org/10.1080/10496475.2021.1947928","url":null,"abstract":"ABSTRACT The biological activities of Inula graveolens in Chebba (Tunisia) salt marsh were screened. Total polyphenol (TPP), flavonoid (TF), and proanthocyanidin (TPC) contents were determined and the correlation between the phytochemistry and the antioxidant, antimicrobial, and anti-enzymatic activities was assessed. The polyphenols in the solvent extracts suggested that salinity stress may enhance the production of bioactive compounds. Antiradical activities and a potent acetylcholinesterase inhibitory power were observed. I. graveolens extracts were antimicrobial against Salmonella typhimurium, Micrococcus luteus, and Staphylococcus aureus. Total flavonoids correlated with the activity against M. luteus, while the total polyphenols content was not correlated with the antimicrobial activity.","PeriodicalId":35803,"journal":{"name":"Journal of Herbs, Spices and Medicinal Plants","volume":"27 1","pages":"426 - 444"},"PeriodicalIF":0.0,"publicationDate":"2021-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/10496475.2021.1947928","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42245370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-10-02DOI: 10.1080/10496475.2021.1941476
M. Kundu, M. Biswas, A. Bhattacharjee, Susmita Das
ABSTRACT The study profiled the volatiles in Mentha longifolia, M. piperita and M. spicata and examined the effect of plant growth regulators (PGRs) - IAA, NaDK and SA on the modulations of volatile organic compounds (VOCs) in M. spicata. GC-MS was used to analyze and identify the compounds in hexane extractions from the selected Mentha sp. Sixteen metabolites belonging to monoterpenes, sesquiterpenes, esters, ethers, ketones, phenols and carbobicyclic compounds were detected. Some important metabolites viz., β-pinene, 1,8-cineol, 1,4-benzenediol, 2,5-dimethyl-, (Z)-β–caryophyllene, cis-β-farnesene, cis-muurola-4 (14),5-diene, germacrene D were found which increased in their relative concentrations compared to control after PGRs treatment.
{"title":"Volatile profiles of three Mentha species and the effect of plant growth regulators (PGRs) on the volatile organic compounds (VOCs) in Mentha spicata","authors":"M. Kundu, M. Biswas, A. Bhattacharjee, Susmita Das","doi":"10.1080/10496475.2021.1941476","DOIUrl":"https://doi.org/10.1080/10496475.2021.1941476","url":null,"abstract":"ABSTRACT The study profiled the volatiles in Mentha longifolia, M. piperita and M. spicata and examined the effect of plant growth regulators (PGRs) - IAA, NaDK and SA on the modulations of volatile organic compounds (VOCs) in M. spicata. GC-MS was used to analyze and identify the compounds in hexane extractions from the selected Mentha sp. Sixteen metabolites belonging to monoterpenes, sesquiterpenes, esters, ethers, ketones, phenols and carbobicyclic compounds were detected. Some important metabolites viz., β-pinene, 1,8-cineol, 1,4-benzenediol, 2,5-dimethyl-, (Z)-β–caryophyllene, cis-β-farnesene, cis-muurola-4 (14),5-diene, germacrene D were found which increased in their relative concentrations compared to control after PGRs treatment.","PeriodicalId":35803,"journal":{"name":"Journal of Herbs, Spices and Medicinal Plants","volume":"27 1","pages":"396 - 410"},"PeriodicalIF":0.0,"publicationDate":"2021-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/10496475.2021.1941476","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48791825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-08-19DOI: 10.1080/10496475.2021.1964012
S. S. Kadapatti, H. N. Murthy
ABSTRACT In vitro propagation method was developed for Andrographis macrobotrys for large-scale multiplication. Nodal explants were cultured on Murashige and Skoog (MS) medium containing cytokinins including 6-benzyl amino purine (BAP), kinetin (KN), 2-isopentyl adenine (2-iP) and thidiazuron (TDZ) at 2.5, 5, 7.5 or 10 μM L−1. Multiple axillary shoots (32 shoots per explants) were regenerated from nodal explants on MS medium supplemented with 7.5 μM L−1 BAP. Quarter strength MS medium supplemented with 5 μM L−1 indole-3-butyric acid (IBA) was effective for induction of roots from the regenerated shoots and on this medium optimal of 49 roots were regenerated from shoots. Micropropagated plants were transplanted to pots containing soil and coco-peat (1:1) mix and maintained in growth chamber at 25 ± 2°C, relative humidity 80%, irradiance of 100 µmol m−2 s−1 for 16-h photoperiod. After eight weeks, plants were transferred to field where soil was mixed with farmyard manure and sand.
{"title":"In Vitro Micropropagation of Andrographis macrobotrys","authors":"S. S. Kadapatti, H. N. Murthy","doi":"10.1080/10496475.2021.1964012","DOIUrl":"https://doi.org/10.1080/10496475.2021.1964012","url":null,"abstract":"ABSTRACT In vitro propagation method was developed for Andrographis macrobotrys for large-scale multiplication. Nodal explants were cultured on Murashige and Skoog (MS) medium containing cytokinins including 6-benzyl amino purine (BAP), kinetin (KN), 2-isopentyl adenine (2-iP) and thidiazuron (TDZ) at 2.5, 5, 7.5 or 10 μM L−1. Multiple axillary shoots (32 shoots per explants) were regenerated from nodal explants on MS medium supplemented with 7.5 μM L−1 BAP. Quarter strength MS medium supplemented with 5 μM L−1 indole-3-butyric acid (IBA) was effective for induction of roots from the regenerated shoots and on this medium optimal of 49 roots were regenerated from shoots. Micropropagated plants were transplanted to pots containing soil and coco-peat (1:1) mix and maintained in growth chamber at 25 ± 2°C, relative humidity 80%, irradiance of 100 µmol m−2 s−1 for 16-h photoperiod. After eight weeks, plants were transferred to field where soil was mixed with farmyard manure and sand.","PeriodicalId":35803,"journal":{"name":"Journal of Herbs, Spices and Medicinal Plants","volume":"28 1","pages":"89 - 98"},"PeriodicalIF":0.0,"publicationDate":"2021-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48996282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-08-17DOI: 10.1080/10496475.2021.1961180
N. Souilah, M. Miara, H. Bendif, K. Medjroubi, J. Snorek
ABSTRACT An ethnobotanical survey was conducted in Emdjez Edchich, Dakkich Rabeh, Sefisfa and Ali Hzila Ramdan of central Russikada province in northeastern Algeria to document the traditional knowledge on medicinal plants used by local populations. Data were analyzed using quantitative indices of sociodemographic data, fidelity level (FL), informant consensus factor (ICF) and use value (UV). A total of 280 participants were interviewed, most of whom were women between the ages of 20 and 40, with a medium level of education. The floristic analysis identified 73 taxa belonging to 43 botanical families, dominated by Lamiaceae and Apiaceae. Gastro-intestinal disease was the most treated and leaves were the most often used in traditional remedies prepared as tea infusions. The highest UV was observed for lentisk (Pistacia lentiscus, UV = 3.4); the highest value for FL was for 54 taxa, of which, 16 were used to treat gastro-intestinal diseases. The calculated ICF showed auditory infections, antiseptics, ocular infections, and neurological disease treatments. Thirty-five new species with 67 new therapeutic uses were identified.
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