Pub Date : 2021-08-17DOI: 10.1080/10496475.2021.1962474
P. Srinivasan, V. Subramaniyan, Thilaka Gk, Karthikeyan Krishnasamy, Sureshkumar Jeyalachagan, Manokar Palani
ABSTRACT The local people of the delta region of Thanjavur (Tamil Nadu, India) were surveyed to explore the therapeutic medicinal plants used in folk medicine in the study area. The people belonged to four agricultural rural villages in the study region. The medicinal plants data was validated using frequency citation (FC), relative frequency of citation (RFC), use value (UV), relative importance (RI), cultural importance index (CIi), informant agreement ratio (IAR), informant consensus factor (IFC) and fidelity level (FL). The survey identified 77 indigenous plants from 43 families, of which Fabaceae was used 12% followed by Rutaceae (6%), Lamiaceae and Cucurbitaceae (each 5%), Solanaceae (5%) and others (63%), used for treating 20 ailment categories. Among plant parts, leaves were the most used in the form of powder. Acorus calamus, Bauhinia racemosa, Borassus flabellifer, Myristica fragrans, Sphaeranthus indicus, Withania somnifera and Cissampelos andromorpha warrant further pharmacological investigations.
{"title":"A Survey on Medicinal Plant Knowledge among the Indigenous Communities (Tamilians) in the Delta Regions of Tamil Nadu, India","authors":"P. Srinivasan, V. Subramaniyan, Thilaka Gk, Karthikeyan Krishnasamy, Sureshkumar Jeyalachagan, Manokar Palani","doi":"10.1080/10496475.2021.1962474","DOIUrl":"https://doi.org/10.1080/10496475.2021.1962474","url":null,"abstract":"ABSTRACT The local people of the delta region of Thanjavur (Tamil Nadu, India) were surveyed to explore the therapeutic medicinal plants used in folk medicine in the study area. The people belonged to four agricultural rural villages in the study region. The medicinal plants data was validated using frequency citation (FC), relative frequency of citation (RFC), use value (UV), relative importance (RI), cultural importance index (CIi), informant agreement ratio (IAR), informant consensus factor (IFC) and fidelity level (FL). The survey identified 77 indigenous plants from 43 families, of which Fabaceae was used 12% followed by Rutaceae (6%), Lamiaceae and Cucurbitaceae (each 5%), Solanaceae (5%) and others (63%), used for treating 20 ailment categories. Among plant parts, leaves were the most used in the form of powder. Acorus calamus, Bauhinia racemosa, Borassus flabellifer, Myristica fragrans, Sphaeranthus indicus, Withania somnifera and Cissampelos andromorpha warrant further pharmacological investigations.","PeriodicalId":35803,"journal":{"name":"Journal of Herbs, Spices and Medicinal Plants","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48049614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-08-12DOI: 10.1080/10496475.2021.1963903
N. Chandra, Gajendra Singh, S. Lingwal, M. Bisht, L. Tewari, V. Joshi
ABSTRACT The ecological status of medicinal and aromatic plants species (MAPs) in the alpine region of the Western Himalaya was assessed to identify the current threats on these MAPs. Of the 400 MAPs reported from the alpine region of Uttarakhand, 50 highly used and commercially exploited MAPs were prioritized for assessment. A semistructured questionnaire survey was used in the representative sites (50) of the alpine landscape based on the information, parts used, locality of occurrence, and socioeconomic status. Field surveys were conducted using Rapid Mapping Exercise (RME) (4 plots of 1 × 1 m at every 50 m distance, total 40 plots in one transect) along the elevation and habitat gradient to assess the population status of species. The intensity of threat was evaluated using a rapid threat assessment (RTA) approach using eight criteria viz., plant parts used, life forms, habitat, endemism, population size, use pattern, trade, and threat status. The recorded magnitude of threats revealed that Angelica glauca, Nardostachys jatamansi, Aconitum heterophyllum, and Rheum australe were the most vulnerable. Roots/rhizomes (68%) were the most frequently used plant parts of MAPs, followed by inflorescence/fruit (19%).
{"title":"Ecological status of Alpine Medicinal and Aromatic Plants of Western Himalaya","authors":"N. Chandra, Gajendra Singh, S. Lingwal, M. Bisht, L. Tewari, V. Joshi","doi":"10.1080/10496475.2021.1963903","DOIUrl":"https://doi.org/10.1080/10496475.2021.1963903","url":null,"abstract":"ABSTRACT The ecological status of medicinal and aromatic plants species (MAPs) in the alpine region of the Western Himalaya was assessed to identify the current threats on these MAPs. Of the 400 MAPs reported from the alpine region of Uttarakhand, 50 highly used and commercially exploited MAPs were prioritized for assessment. A semistructured questionnaire survey was used in the representative sites (50) of the alpine landscape based on the information, parts used, locality of occurrence, and socioeconomic status. Field surveys were conducted using Rapid Mapping Exercise (RME) (4 plots of 1 × 1 m at every 50 m distance, total 40 plots in one transect) along the elevation and habitat gradient to assess the population status of species. The intensity of threat was evaluated using a rapid threat assessment (RTA) approach using eight criteria viz., plant parts used, life forms, habitat, endemism, population size, use pattern, trade, and threat status. The recorded magnitude of threats revealed that Angelica glauca, Nardostachys jatamansi, Aconitum heterophyllum, and Rheum australe were the most vulnerable. Roots/rhizomes (68%) were the most frequently used plant parts of MAPs, followed by inflorescence/fruit (19%).","PeriodicalId":35803,"journal":{"name":"Journal of Herbs, Spices and Medicinal Plants","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41647040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-07-14DOI: 10.1080/10496475.2021.1947431
Subhandu Patra, Shrishti Sharma
ABSTRACT The present study examined the Gond tribals for traditional knowledge over ethnomedicinal practices with an emphasis on its documentation, healing practices, and usages. Empirical fieldwork was conducted in Bilaspur district, of Chhattisgarh, India using semistructured interview, observation, and case study involving 30 key informants selected through purposive sampling. The study documented 65 medicinal plants species belonging to 36 families. . of which 37% species were trees, 28% herbs, 20% small trees, 6% climbers, and 9% species shrubs being used for treating 61 types of disease. Datura stramonium was most used plant by the traditional healers. Additionally, 23 plant species were also used as edibles.
{"title":"Contemporary Ethnomedicinal Practices among the Gond Tribe of Bilaspur District, Chhattisgarh, India","authors":"Subhandu Patra, Shrishti Sharma","doi":"10.1080/10496475.2021.1947431","DOIUrl":"https://doi.org/10.1080/10496475.2021.1947431","url":null,"abstract":"ABSTRACT The present study examined the Gond tribals for traditional knowledge over ethnomedicinal practices with an emphasis on its documentation, healing practices, and usages. Empirical fieldwork was conducted in Bilaspur district, of Chhattisgarh, India using semistructured interview, observation, and case study involving 30 key informants selected through purposive sampling. The study documented 65 medicinal plants species belonging to 36 families. . of which 37% species were trees, 28% herbs, 20% small trees, 6% climbers, and 9% species shrubs being used for treating 61 types of disease. Datura stramonium was most used plant by the traditional healers. Additionally, 23 plant species were also used as edibles.","PeriodicalId":35803,"journal":{"name":"Journal of Herbs, Spices and Medicinal Plants","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/10496475.2021.1947431","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42143997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-07-03DOI: 10.1080/10496475.2020.1806166
Annapurna Annayappa Shanthala, Abhirami Dilkalal, T. Umesh
ABSTRACT An in vitro protocol for direct and callus mediated regeneration of complete plantlets of Curcuma karnatakensis was established. For direct regeneration rhizome buds were used as explants and culturing them on MS medium supplemented with 2 mgL−1 6-Benzyladenine (BA) resulted with an average of 4.00 ± 0.57 shoots per explant. Callus induction and multiplication study revealed that the percentage of callus induction was better in rhizome explants (68% explants induced callus within 30 d of inoculation) compared to shoot base explants (54% explants induced in 60 d) on MS medium supplemented with 2 mgL−1 2,4-Dichlorophenoxyacetic acid. Rhizome derived calli were used to induce organogenic callus on medium with higher concentration of α-Naphthalene acetic acid (5 mgL−1) and further transferred to regeneration medium containing 0.5 mgL−1 NAA and 4mgL−1 BA which produced 5.33 ± 0.66 shoots per explant in 8 weeks of culture. In vitro regenerated plants were successfully acclimatized to greenhouse conditions with a survival rate of 85% and exhibited normal phenotypes.
{"title":"An Efficient Invitro Approach for Direct and Callus Mediated Regeneration of Curcuma karnatakensis – An Endemic Plant of Karnataka","authors":"Annapurna Annayappa Shanthala, Abhirami Dilkalal, T. Umesh","doi":"10.1080/10496475.2020.1806166","DOIUrl":"https://doi.org/10.1080/10496475.2020.1806166","url":null,"abstract":"ABSTRACT An in vitro protocol for direct and callus mediated regeneration of complete plantlets of Curcuma karnatakensis was established. For direct regeneration rhizome buds were used as explants and culturing them on MS medium supplemented with 2 mgL−1 6-Benzyladenine (BA) resulted with an average of 4.00 ± 0.57 shoots per explant. Callus induction and multiplication study revealed that the percentage of callus induction was better in rhizome explants (68% explants induced callus within 30 d of inoculation) compared to shoot base explants (54% explants induced in 60 d) on MS medium supplemented with 2 mgL−1 2,4-Dichlorophenoxyacetic acid. Rhizome derived calli were used to induce organogenic callus on medium with higher concentration of α-Naphthalene acetic acid (5 mgL−1) and further transferred to regeneration medium containing 0.5 mgL−1 NAA and 4mgL−1 BA which produced 5.33 ± 0.66 shoots per explant in 8 weeks of culture. In vitro regenerated plants were successfully acclimatized to greenhouse conditions with a survival rate of 85% and exhibited normal phenotypes.","PeriodicalId":35803,"journal":{"name":"Journal of Herbs, Spices and Medicinal Plants","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/10496475.2020.1806166","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46059066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-07-03DOI: 10.1080/10496475.2021.1911905
Walaa H El-Maadawy, S. S. Seif el-Din, S. Ezzat, O. Hammam, M. M. Safar, S. Saleh, N. El-Lakkany
ABSTRACT Despite rutin, extracted from black mulberry, has several pharmacological activities, its exact effect against hepatic fibrosis remains incompletely identified. Accordingly, this study investigates whether rutin is a promising candidate for treating hepatic fibrosis and to clarify its underlying antifibrotic mechanisms in vitro and in vivo. In vitro studies were performed on hepatic stellate cell line (HSC-T6) whereas liver fibrosis was established in rats via chronic thioacetamide (TAA)-intoxication. Rats were divided into (i) normal, (ii) TAA-intoxicated rats; TAA-intoxicated rats treated with (iii) silymarin or (iv) rutin. Levels of ALT, AST, platelet-derived growth factor-BB (PDGF-BB), tissue inhibitor metalloproteinases type-1 (TIMP-1), hydroxyproline and expression of proliferating cellular nuclear antigen (PCNA) together with histological changes were examined. Activities of rutin on TGF-β1, α-smooth muscle actin (α-SMA) and caspase-3 were measured in vitro and in vivo. Rutin exhibited no marked HSC-T6 cell death (IC50 = 460 µg.ml−1), however, it showed reduction in HSCs activation (low TGF-β1 level and α-SMA positive cells) and induced apoptosis (high caspase-3 positive cells). Rutin also ameliorated liver functions, reduced hepatic levels of PDGF-BB, TGF-β1, TIMP-1, hydroxyproline and restored PCNA, together with attenuation in fibrosis score (S1 vs S4). Rutin could be a promising candidate for treating hepatic fibrosis through down-regulation of HSCs activation and induction of apoptosis.
摘要尽管从黑桑椹中提取的芦丁具有多种药理活性，但其抗肝纤维化的确切作用尚不清楚。因此，本研究调查了芦丁是否是治疗肝纤维化的有前途的候选药物，并阐明了其在体外和体内潜在的抗纤维化机制。对肝星状细胞系（HSC-T6）进行了体外研究，而通过慢性硫代乙酰胺（TAA）中毒在大鼠中建立了肝纤维化。将大鼠分为（i）正常大鼠、（ii）TAA中毒大鼠；用（iii）水飞蓟素或（iv）芦丁处理的TAA中毒大鼠。检测ALT、AST、血小板衍生生长因子BB（PDGF-BB）、组织抑制剂金属蛋白酶-1（TIMP-1）、羟脯氨酸和增殖细胞核抗原（PCNA）的表达水平以及组织学变化。在体外和体内测定芦丁对TGF-β1、α-平滑肌肌动蛋白（α-SMA）和胱天蛋白酶-3的活性。芦丁没有表现出明显的HSC-T6细胞死亡（IC50=460µg.ml−1），但它显示出HSC活化减少（低TGF-β1水平和α-SMA阳性细胞）和诱导凋亡（高胱天蛋白酶-3阳性细胞）。芦丁还改善了肝功能，降低了肝脏PDGF-BB、TGF-β1、TIMP-1、羟脯氨酸的水平，并恢复了PCNA，同时降低了纤维化评分（S1 vs S4）。芦丁可以通过下调HSC的激活和诱导细胞凋亡来治疗肝纤维化，这是一种很有前途的候选药物。
{"title":"Rutin Ameliorates Hepatic Fibrosis via Targeting Hepatic Stellate Cells’ Activation, Proliferation and Apoptosis","authors":"Walaa H El-Maadawy, S. S. Seif el-Din, S. Ezzat, O. Hammam, M. M. Safar, S. Saleh, N. El-Lakkany","doi":"10.1080/10496475.2021.1911905","DOIUrl":"https://doi.org/10.1080/10496475.2021.1911905","url":null,"abstract":"ABSTRACT Despite rutin, extracted from black mulberry, has several pharmacological activities, its exact effect against hepatic fibrosis remains incompletely identified. Accordingly, this study investigates whether rutin is a promising candidate for treating hepatic fibrosis and to clarify its underlying antifibrotic mechanisms in vitro and in vivo. In vitro studies were performed on hepatic stellate cell line (HSC-T6) whereas liver fibrosis was established in rats via chronic thioacetamide (TAA)-intoxication. Rats were divided into (i) normal, (ii) TAA-intoxicated rats; TAA-intoxicated rats treated with (iii) silymarin or (iv) rutin. Levels of ALT, AST, platelet-derived growth factor-BB (PDGF-BB), tissue inhibitor metalloproteinases type-1 (TIMP-1), hydroxyproline and expression of proliferating cellular nuclear antigen (PCNA) together with histological changes were examined. Activities of rutin on TGF-β1, α-smooth muscle actin (α-SMA) and caspase-3 were measured in vitro and in vivo. Rutin exhibited no marked HSC-T6 cell death (IC50 = 460 µg.ml−1), however, it showed reduction in HSCs activation (low TGF-β1 level and α-SMA positive cells) and induced apoptosis (high caspase-3 positive cells). Rutin also ameliorated liver functions, reduced hepatic levels of PDGF-BB, TGF-β1, TIMP-1, hydroxyproline and restored PCNA, together with attenuation in fibrosis score (S1 vs S4). Rutin could be a promising candidate for treating hepatic fibrosis through down-regulation of HSCs activation and induction of apoptosis.","PeriodicalId":35803,"journal":{"name":"Journal of Herbs, Spices and Medicinal Plants","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/10496475.2021.1911905","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49325830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-14DOI: 10.1080/10496475.2021.1932006
R. Kolap, Prachi S. Kakade, R. Gacche, S. Zimare
ABSTRACT The antioxidant potential (% RSA and EC50) of solvent extracts of leaves and roots of Lobelia nicotianifolia Roth. was assessed using five in vitro assays. The EC50 values of methanolic extract were comparable to that of ascorbic acid for nitric oxide (30.67 µg mL−1) and hydrogen peroxide (81.01 µg mL−1) radical assays. The % RSA and EC50 correlated with the total phenolic content and total flavonoid content. The characterization by liquid chromatography-high-resolution mass spectrometry showed the presence of five flavonoids, six phenolics, three carotenoids, one anthraquinone, coumarin, hydroxyquinone, and isoflavonoid. Embelin, gallic acid, and quercetin were quantified by high-performance liquid chromatography.
{"title":"Assessment of Radical Scavenging Activity and Estimation of EC50 Values of Various Extracts of Leaves and Roots from Lobelia nicotianifolia Roth. (Wild Tobacco)","authors":"R. Kolap, Prachi S. Kakade, R. Gacche, S. Zimare","doi":"10.1080/10496475.2021.1932006","DOIUrl":"https://doi.org/10.1080/10496475.2021.1932006","url":null,"abstract":"ABSTRACT The antioxidant potential (% RSA and EC50) of solvent extracts of leaves and roots of Lobelia nicotianifolia Roth. was assessed using five in vitro assays. The EC50 values of methanolic extract were comparable to that of ascorbic acid for nitric oxide (30.67 µg mL−1) and hydrogen peroxide (81.01 µg mL−1) radical assays. The % RSA and EC50 correlated with the total phenolic content and total flavonoid content. The characterization by liquid chromatography-high-resolution mass spectrometry showed the presence of five flavonoids, six phenolics, three carotenoids, one anthraquinone, coumarin, hydroxyquinone, and isoflavonoid. Embelin, gallic acid, and quercetin were quantified by high-performance liquid chromatography.","PeriodicalId":35803,"journal":{"name":"Journal of Herbs, Spices and Medicinal Plants","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/10496475.2021.1932006","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48169417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-14DOI: 10.1080/10496475.2021.1934215
K. Akinwunmi, C. Amadi, M. Awodiran
ABSTRACT This study evaluated the ameliorative potential of the aqueous leaf extracts of Pterocarpus mildbraedii against crude oil-induced toxicity in rats. The effects of co-administration of the P. mildbraedii leaf extract with diets contaminated with water-soluble fraction of crude oil on biochemical and chromosomal indices in albino rats were studied. Rats were fed with the crude oil-contaminated diets and administered the aqueous P. mildbraedii leaf extract at 100, 300, or 500 mg kg−1 bwt for 6 weeks. The activities of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), and creatinine kinase-MB (CK-MB) as well as the concentrations of total protein, albumin, total bilirubin, creatinine, and urea in the plasma of experimental rats were estimated. Bone marrow cells obtained from the femurs of the rats were scored for chromosomal aberrations. Results showed that the administration of the P. mildbraedii leaf extract restored the levels of the plasma enzymes, blood proteins, urea, and creatinine close to normal control levels, which were altered by the crude oil-contaminated diet, and the number of chromosomal aberrations were fewer in treated rats than in the untreated intoxicated rats.
{"title":"The effect of aqueous leaf extracts of Pterocarpus mildbraedii on crude oil-induced toxicity in Albino rats","authors":"K. Akinwunmi, C. Amadi, M. Awodiran","doi":"10.1080/10496475.2021.1934215","DOIUrl":"https://doi.org/10.1080/10496475.2021.1934215","url":null,"abstract":"ABSTRACT This study evaluated the ameliorative potential of the aqueous leaf extracts of Pterocarpus mildbraedii against crude oil-induced toxicity in rats. The effects of co-administration of the P. mildbraedii leaf extract with diets contaminated with water-soluble fraction of crude oil on biochemical and chromosomal indices in albino rats were studied. Rats were fed with the crude oil-contaminated diets and administered the aqueous P. mildbraedii leaf extract at 100, 300, or 500 mg kg−1 bwt for 6 weeks. The activities of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), and creatinine kinase-MB (CK-MB) as well as the concentrations of total protein, albumin, total bilirubin, creatinine, and urea in the plasma of experimental rats were estimated. Bone marrow cells obtained from the femurs of the rats were scored for chromosomal aberrations. Results showed that the administration of the P. mildbraedii leaf extract restored the levels of the plasma enzymes, blood proteins, urea, and creatinine close to normal control levels, which were altered by the crude oil-contaminated diet, and the number of chromosomal aberrations were fewer in treated rats than in the untreated intoxicated rats.","PeriodicalId":35803,"journal":{"name":"Journal of Herbs, Spices and Medicinal Plants","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/10496475.2021.1934215","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44248825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-08DOI: 10.1080/10496475.2021.1934620
Anh Thi Van Pham, Minh Hien Luong, Hang Thi Thu Dinh, Thanh Phuong Mai, Q. Trinh, L. H. Luong
ABSTRACT In this study, the effect of Moringa oleifera extracts were investigated on an immunosuppressed model of cyclophosphamide-induced Swiss mice. M. oleifera seed and root extracts (2.0 g kg−1 body wt) were administered orally for 7 consecutive days; cyclophosphamide (CP) (200 mg kg−1 i.p.) was administered by IV on the 4th day. Results indicated that the extracts of the M. oleifera seeds and roots ameliorated the immunosuppressive effects of CP on delayed type hypersensitivity (DTH) response, leukocyte counts, relative organ weight, IgM concentration, serum TNF-α assay and micro-histological images. This study demonstrated in vivo the therapeutic effect of M. oleifera on the immune response in clinical application.
摘要本研究采用环磷酰胺诱导的瑞士小鼠免疫抑制模型，研究辣木提取物的作用。连续7天口服M.oleifera种子和根提取物（2.0 g kg−1体重）；第4天静脉注射环磷酰胺（CP）（200 mg kg−1 i.p.）。结果表明，油籽和油根提取物改善了CP对迟发型超敏反应、白细胞计数、相对器官重量、IgM浓度、血清TNF-α测定和显微组织学图像的免疫抑制作用。本研究在体内证明了油在临床应用中对免疫反应的治疗作用。
{"title":"Immunostimulatory Effect of Moringa oleifera Extracts on Cyclophosphamide-induced Immunosuppressed Mice","authors":"Anh Thi Van Pham, Minh Hien Luong, Hang Thi Thu Dinh, Thanh Phuong Mai, Q. Trinh, L. H. Luong","doi":"10.1080/10496475.2021.1934620","DOIUrl":"https://doi.org/10.1080/10496475.2021.1934620","url":null,"abstract":"ABSTRACT In this study, the effect of Moringa oleifera extracts were investigated on an immunosuppressed model of cyclophosphamide-induced Swiss mice. M. oleifera seed and root extracts (2.0 g kg−1 body wt) were administered orally for 7 consecutive days; cyclophosphamide (CP) (200 mg kg−1 i.p.) was administered by IV on the 4th day. Results indicated that the extracts of the M. oleifera seeds and roots ameliorated the immunosuppressive effects of CP on delayed type hypersensitivity (DTH) response, leukocyte counts, relative organ weight, IgM concentration, serum TNF-α assay and micro-histological images. This study demonstrated in vivo the therapeutic effect of M. oleifera on the immune response in clinical application.","PeriodicalId":35803,"journal":{"name":"Journal of Herbs, Spices and Medicinal Plants","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/10496475.2021.1934620","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41998112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-07DOI: 10.1080/10496475.2021.1935385
M. K. Chaudhary, Ankita Misra, U. Shankar, P. Agnihotri, S. Srivastava
ABSTRACT The metabolic variation of berberine and palmatine content in the roots of Berberis lycium was analyzed by HPTLC–UV for the identification of quality raw material (QRM) used in the industry. Ten natural population(s) were mapped, and samples were collected from different parts of Western Himalayas. Pharmacognostical parameters of each collected raw material were evaluated as per Ayurvedic pharmacopoeia of India (API) for quality. The HPTLC chromatogram was developed in n-propanol-water-formic acid (9:1:0.1 v/v/v) and quantified at λmax of 350 nm. Rf of palmatine and berberine was at 0.2 ± 0.08 and 0.33 ± 0.03, respectively, and was linear at a concentration range of 0.1–0.5 µg per spot for both markers. Limit of detection (LOD) and limit of quantification (LOQ) of palmatine and berberine were 5.25, 5.23 and 15.91, 15.86 ng per spot, respectively. Palmatine and berberine varied from 0.117% to 0.739% and 0.203% to 1.134% on dry weight basis. NB-03 was identified as an elite source based on cluster analysis of metabolic content(s).
{"title":"Intra-specific Variation of Alkaloid Content in Berberis lycium from the Western Himalaya","authors":"M. K. Chaudhary, Ankita Misra, U. Shankar, P. Agnihotri, S. Srivastava","doi":"10.1080/10496475.2021.1935385","DOIUrl":"https://doi.org/10.1080/10496475.2021.1935385","url":null,"abstract":"ABSTRACT The metabolic variation of berberine and palmatine content in the roots of Berberis lycium was analyzed by HPTLC–UV for the identification of quality raw material (QRM) used in the industry. Ten natural population(s) were mapped, and samples were collected from different parts of Western Himalayas. Pharmacognostical parameters of each collected raw material were evaluated as per Ayurvedic pharmacopoeia of India (API) for quality. The HPTLC chromatogram was developed in n-propanol-water-formic acid (9:1:0.1 v/v/v) and quantified at λmax of 350 nm. Rf of palmatine and berberine was at 0.2 ± 0.08 and 0.33 ± 0.03, respectively, and was linear at a concentration range of 0.1–0.5 µg per spot for both markers. Limit of detection (LOD) and limit of quantification (LOQ) of palmatine and berberine were 5.25, 5.23 and 15.91, 15.86 ng per spot, respectively. Palmatine and berberine varied from 0.117% to 0.739% and 0.203% to 1.134% on dry weight basis. NB-03 was identified as an elite source based on cluster analysis of metabolic content(s).","PeriodicalId":35803,"journal":{"name":"Journal of Herbs, Spices and Medicinal Plants","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/10496475.2021.1935385","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42881174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-04-15DOI: 10.1080/10496475.2021.1911904
Siti Pauliena Mohd Bohari, L. S. Chua, N. Adrus, Z. Rahmat, Hafedh Ahmed Abdullah Al-Moalemi
ABSTRACT This study investigated the phytochemicals, protein and cellulose profiles, and the antiradical, antibacterial and cytotoxicity activities of Orthosiphon aristatus. The cellulose profile of the herb had functional groups of C-H and C-O stretching, C = O ester, CH2 bending and scissoring using alkaline treatment and bleaching process. Rosmarinic acid was the most dominant compound in the plant extract and its fractions prepared from reflux extraction followed by column chromatography for sample clean-up. At least 8 protein bands could be seen from the polyacrylamide gel and transketolase with the molecular size, 73 kDa was identified. The antiradical activity of the protein fraction increased after trypsin hydrolysis. The antiradical activities of crude extract and fraction 2 achieved up to 90% inhibition at 80 and 40 µg mL−1, respectively. Fractionation increased the rosmarinic acid concentration, thus increasing the antioxidant capacity of fraction. Rosmarinic acid was the main contributor inhibiting the growth of Pseudomonas aeruginosa. Both crude extract and its fraction were non-cytotoxic as the 50% cell viability was 565.6 and 150.5 µg mL−1, respectively, using human fibroblasts.
{"title":"Biochemical Characterization of Orthosiphon Aristatus and Evaluation of Pharmacological Activities","authors":"Siti Pauliena Mohd Bohari, L. S. Chua, N. Adrus, Z. Rahmat, Hafedh Ahmed Abdullah Al-Moalemi","doi":"10.1080/10496475.2021.1911904","DOIUrl":"https://doi.org/10.1080/10496475.2021.1911904","url":null,"abstract":"ABSTRACT This study investigated the phytochemicals, protein and cellulose profiles, and the antiradical, antibacterial and cytotoxicity activities of Orthosiphon aristatus. The cellulose profile of the herb had functional groups of C-H and C-O stretching, C = O ester, CH2 bending and scissoring using alkaline treatment and bleaching process. Rosmarinic acid was the most dominant compound in the plant extract and its fractions prepared from reflux extraction followed by column chromatography for sample clean-up. At least 8 protein bands could be seen from the polyacrylamide gel and transketolase with the molecular size, 73 kDa was identified. The antiradical activity of the protein fraction increased after trypsin hydrolysis. The antiradical activities of crude extract and fraction 2 achieved up to 90% inhibition at 80 and 40 µg mL−1, respectively. Fractionation increased the rosmarinic acid concentration, thus increasing the antioxidant capacity of fraction. Rosmarinic acid was the main contributor inhibiting the growth of Pseudomonas aeruginosa. Both crude extract and its fraction were non-cytotoxic as the 50% cell viability was 565.6 and 150.5 µg mL−1, respectively, using human fibroblasts.","PeriodicalId":35803,"journal":{"name":"Journal of Herbs, Spices and Medicinal Plants","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/10496475.2021.1911904","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49125655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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