Colorectal cancer is the third most common cancer worldwide and preventative methods are currently desired. Oligonol derived from lychee is a polyphenol that reported to improve lifestyle-related diseases, but its effect on intestinal carcinogenesis has not been clarified. Thus, we investigated the effects of oligonol on Wnt/β-catenin signaling pathway in vitro and its effects on intestinal polyp development in vivo using Apc-mutant mice. As a result, oligonol treatment inhibited TCF/LEF reporter activity through non-phosphorylated β-catenin (active form) degradation in human colon cancer cells. Apc-mutant mice given oligonol for 8 weeks resulted in the suppression of intestinal polyp development along with inhibition of mRNA levels of c-Myc and LGR5, which are both regulated by the TCF/LEF transcription factor. Moreover, reduction of serum triglyceride levels was observed after oligonol administration. It is expected that polyphenols from lychee may become a new natural ingredient for cancer prevention in the future.
{"title":"Oligonol inhibits mouse intestinal tumorigenesis through Wnt signal regulation","authors":"Takahiro Hamoya, Shingo Miyamoto, Ryo Arita, Suzuko Ohsako, Michihiro Mutoh","doi":"10.1016/j.jff.2025.107100","DOIUrl":"10.1016/j.jff.2025.107100","url":null,"abstract":"<div><div>Colorectal cancer is the third most common cancer worldwide and preventative methods are currently desired. Oligonol derived from lychee is a polyphenol that reported to improve lifestyle-related diseases, but its effect on intestinal carcinogenesis has not been clarified. Thus, we investigated the effects of oligonol on Wnt/β-catenin signaling pathway <em>in vitro</em> and its effects on intestinal polyp development <em>in vivo</em> using <em>Apc</em>-mutant mice<em>.</em> As a result, oligonol treatment inhibited TCF/LEF reporter activity through non-phosphorylated β-catenin (active form) degradation in human colon cancer cells. <em>Apc</em>-mutant mice given oligonol for 8 weeks resulted in the suppression of intestinal polyp development along with inhibition of mRNA levels of <em>c-Myc</em> and <em>LGR5</em>, which are both regulated by the TCF/LEF transcription factor. Moreover, reduction of serum triglyceride levels was observed after oligonol administration. It is expected that polyphenols from lychee may become a new natural ingredient for cancer prevention in the future.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"135 ","pages":"Article 107100"},"PeriodicalIF":4.0,"publicationDate":"2025-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145517587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-11-10DOI: 10.1016/j.jff.2025.107092
Anastasia V. Romanov , Tyler N. Graf , Warren Vidar , Dereje D. Jima , Nicholas H. Oberlies , Zhenquan Jia , Ayalew Ligaba-Osena
Brown tef (Eragrostis tef) grain extracts increase cellular glutathione (GSH) in THP-1 monocytes, however, the molecular mechanisms remained unclear. In this study, we found that the tef extracts induced luciferase activity by 19-fold in HEK293 cells stably expressing the luciferase gene under the control of the nuclear factor erythroid 2-related factor 2 (Nrf2)–antioxidant response element, indicating the activation of the antioxidant pathway. RNA-seq analysis revealed that the tef extract enhanced the expression of 87 genes, including some regulated by the Nrf2 transcription factor. Differentially expressed genes included ferritin heavy chain pseudogenes, tumor necrosis factor (TNF)-α, and solute carrier genes. Using LC-MS, we identified compounds that were differentially enriched in the active tef fraction as compared to the inactive fraction, including vernolic and α-linolenic acid, which were enhanced by over 60-fold. In summary, our findings indicate that tef grains contain phytochemicals that enhance the antioxidant levels through the Nrf2-signaling pathway.
{"title":"Studying the molecular mechanisms of tef (Eragrostis tef) antioxidant properties in human THP-1 monocytes","authors":"Anastasia V. Romanov , Tyler N. Graf , Warren Vidar , Dereje D. Jima , Nicholas H. Oberlies , Zhenquan Jia , Ayalew Ligaba-Osena","doi":"10.1016/j.jff.2025.107092","DOIUrl":"10.1016/j.jff.2025.107092","url":null,"abstract":"<div><div>Brown tef (<em>Eragrostis tef</em>) grain extracts increase cellular glutathione (GSH) in THP-1 monocytes, however, the molecular mechanisms remained unclear. In this study, we found that the tef extracts induced luciferase activity by 19-fold in HEK293 cells stably expressing the luciferase gene under the control of the nuclear factor erythroid 2-related factor 2 (Nrf2)–antioxidant response element, indicating the activation of the antioxidant pathway. RNA-seq analysis revealed that the tef extract enhanced the expression of 87 genes, including some regulated by the Nrf2 transcription factor. Differentially expressed genes included <em>ferritin heavy chain pseudogenes,</em> tumor necrosis factor (TNF)-α, and <em>solute carrier</em> genes. Using LC-MS, we identified compounds that were differentially enriched in the active tef fraction as compared to the inactive fraction, including vernolic and α-linolenic acid, which were enhanced by over 60-fold. In summary, our findings indicate that tef grains contain phytochemicals that enhance the antioxidant levels through the Nrf2-signaling pathway.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"135 ","pages":"Article 107092"},"PeriodicalIF":4.0,"publicationDate":"2025-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145517586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-11-09DOI: 10.1016/j.jff.2025.107094
Han Yang , Yansong Gao , Fang Li , Chenli Chu , Lingli Gong , Songyi Lin , Zhiqiang Lu , Lingming Kong
Walnut protein hydrolysates exert neuroprotection. This study further evaluated hydrolysates from walnut albumin, globulin, prolamin, glutelin by alkaline, neutral protease, and trypsin by detecting radical-scavenging and anti-oxidant/inflammatory activities in lipopolysaccharide-stimulated BV2 microglia. Further, novel memory-enhancing bioactive peptides were identified by in-silico experiments and cell validation. Among hydrolysates, alkaline protease-derived glutelin hydrolysates (WAGluP) exhibited strongest bioactivity, showing higher radical-scavenging capacity, increased the activities of superoxide-dismutase (SOD) and glutathione-peroxidase (GSH-PX) by 19.961 and 4.359 U/mg prot, and reduced nitric-oxide (NO, −13.129 μmol/g prot) production. Moreover, WAGluP decreased tumor-necrosis-factor-α (TNF-α, −21.2 %) and interleukin-6 (IL-6, −12.3 %) levels. Additionally, LWPF, WLPF, and YFPF were identified as potential memory-enhancing candidates by peptidomic, PeptideRanker, and molecular docking from WAGluP, in which LWPF and WLPF increased SOD activity by 71.9 and 54.01 %, and GSH-PX activity by 87.32 and 99.12 %. Overall, WAGluP and its peptides exert multitarget antioxidant and anti-inflammatory effects with memory-enhancing potential, supporting walnut-glutelin-peptides for neuroprotective functional-foods.
{"title":"Walnut (Juglans regia L.) glutelin-derived peptides as novel candidates for cognitive enhancement: targeting oxidative stress and neuroinflammation","authors":"Han Yang , Yansong Gao , Fang Li , Chenli Chu , Lingli Gong , Songyi Lin , Zhiqiang Lu , Lingming Kong","doi":"10.1016/j.jff.2025.107094","DOIUrl":"10.1016/j.jff.2025.107094","url":null,"abstract":"<div><div>Walnut protein hydrolysates exert neuroprotection. This study further evaluated hydrolysates from walnut albumin, globulin, prolamin, glutelin by alkaline, neutral protease, and trypsin by detecting radical-scavenging and anti-oxidant/inflammatory activities in lipopolysaccharide-stimulated BV2 microglia. Further, novel memory-enhancing bioactive peptides were identified by in-silico experiments and cell validation. Among hydrolysates, alkaline protease-derived glutelin hydrolysates (WAGluP) exhibited strongest bioactivity, showing higher radical-scavenging capacity, increased the activities of superoxide-dismutase (SOD) and glutathione-peroxidase (GSH-PX) by 19.961 and 4.359 U/mg prot, and reduced nitric-oxide (NO, −13.129 μmol/g prot) production. Moreover, WAGluP decreased tumor-necrosis-factor-α (TNF-α, −21.2 %) and interleukin-6 (IL-6, −12.3 %) levels.<!--> <!--> Additionally, LWPF, WLPF, and YFPF were identified as potential memory-enhancing candidates by peptidomic, PeptideRanker, and molecular docking from WAGluP, in which LWPF and WLPF increased SOD activity by 71.9 and 54.01 %, and GSH-PX activity by 87.32 and 99.12 %. Overall, WAGluP and its peptides exert multitarget antioxidant and anti-inflammatory effects with memory-enhancing potential, supporting walnut-glutelin-peptides for neuroprotective functional-foods.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"135 ","pages":"Article 107094"},"PeriodicalIF":4.0,"publicationDate":"2025-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145517588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-11-09DOI: 10.1016/j.jff.2025.107095
Rodrigue Brossaud , Valentine S. Moullé , Marina Giblaine , Sergine Even , Marie-Bernadette Maillard , Séverine Chevalier , Thibauld Oullier , Eric Guédon , Yves Le Loir , Moustapha Cissé , Hélène Falentin , Michel Neunlist
Amyloid-ß (Aß) induced alterations in gut functions, such as reduced motility and compromised barrier integrity contribute to age-related frailty. Therefore, developing fermentation-based products to prevent age-related gut disorders represents a major therapeutic interest. Primary cultures of enteric nervous system and Caco-2 monolayers were exposed to Aß oligomers following pretreatment with individual or a combination of candidate microbial derived metabolites (butyrate, gamma-aminobutyric acid, and indole derivatives), or a candidate metabolite-enriched fermented dairy product that we subsequently developed. Results showed that individual metabolites significantly preserved markers of neuronal connectivity (beta-tubulin III and synaptophysin) and stabilized tight junction proteins (zonula occludens-1 and occludin), reducing Aß-induced dysfunction. Furthermore, enhanced protective effects of the fermented dairy products enriched with microbial metabolites were observed, suggesting a promising dietary strategy for supporting gut health in aging and Alzheimer's disease, although in vivo validation is needed.
{"title":"Microbial metabolites alleviate Aß-induced alterations of intestinal epithelial barrier and enteric nervous system connectivity","authors":"Rodrigue Brossaud , Valentine S. Moullé , Marina Giblaine , Sergine Even , Marie-Bernadette Maillard , Séverine Chevalier , Thibauld Oullier , Eric Guédon , Yves Le Loir , Moustapha Cissé , Hélène Falentin , Michel Neunlist","doi":"10.1016/j.jff.2025.107095","DOIUrl":"10.1016/j.jff.2025.107095","url":null,"abstract":"<div><div>Amyloid-ß (Aß) induced alterations in gut functions, such as reduced motility and compromised barrier integrity contribute to age-related frailty. Therefore, developing fermentation-based products to prevent age-related gut disorders represents a major therapeutic interest. Primary cultures of enteric nervous system and Caco-2 monolayers were exposed to Aß oligomers following pretreatment with individual or a combination of candidate microbial derived metabolites (butyrate, gamma-aminobutyric acid, and indole derivatives), or a candidate metabolite-enriched fermented dairy product that we subsequently developed. Results showed that individual metabolites significantly preserved markers of neuronal connectivity (beta-tubulin III and synaptophysin) and stabilized tight junction proteins (zonula occludens-1 and occludin), reducing Aß-induced dysfunction. Furthermore, enhanced protective effects of the fermented dairy products enriched with microbial metabolites were observed, suggesting a promising dietary strategy for supporting gut health in aging and Alzheimer's disease, although in vivo validation is needed.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"135 ","pages":"Article 107095"},"PeriodicalIF":4.0,"publicationDate":"2025-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145517585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-11-08DOI: 10.1016/j.jff.2025.107096
Lu Zhou , Wen Jiang , Fei Pei , Yang Ji , Anxiang Su , Gaoxing Ma , Yimeng Zhang , Qiuhui Hu , Ning Ma
P.eryngii extruded rice (PEER) has been demonstrated to be a medium glycemic index food. To investigate its hypoglycemic potential, this study established a type 2 diabetic mouse model using streptozotocin (STZ) combined with a high-fat diet, followed by an 8 wk. dietary intervention. The results showed that, compared to the model control (MC) group, PEER intervention significantly lowered blood glucose, improved oral glucose tolerance (OGTT), reduced blood lipids (TC, TG, and LDL-C decreased by 18.22 %, 19.65 %, and 52.25 %, respectively) and insulin levels. In addition, PEER significantly upregulated the relative expression of IR, IRS-1, IRS-2, PI3K, and GSK-3β genes in the liver, altered the composition and structural proportion of gut microbiota in mice and notably increased the abundance of beneficial bacteria such as Akkermansia. In conclusion, PEER exhibited a significant hypoglycemic effect in type 2 diabetic mice, providing a theoretical basis for developing P. eryngii-based hypoglycemic products.
{"title":"Pleurotus eryngii extruded rice improved HFD/STZ-induced type 2 diabetic mice by activating PI3K/AKT signaling pathway and regulating gut microbiota","authors":"Lu Zhou , Wen Jiang , Fei Pei , Yang Ji , Anxiang Su , Gaoxing Ma , Yimeng Zhang , Qiuhui Hu , Ning Ma","doi":"10.1016/j.jff.2025.107096","DOIUrl":"10.1016/j.jff.2025.107096","url":null,"abstract":"<div><div><em>P.eryngii</em> extruded rice (PEER) has been demonstrated to be a medium glycemic index food. To investigate its hypoglycemic potential, this study established a type 2 diabetic mouse model using streptozotocin (STZ) combined with a high-fat diet, followed by an 8 wk. dietary intervention. The results showed that, compared to the model control (MC) group, PEER intervention significantly lowered blood glucose, improved oral glucose tolerance (OGTT), reduced blood lipids (TC, TG, and LDL-C decreased by 18.22 %, 19.65 %, and 52.25 %, respectively) and insulin levels. In addition, PEER significantly upregulated the relative expression of IR, IRS-1, IRS-2, PI3K, and GSK-3β genes in the liver, altered the composition and structural proportion of gut microbiota in mice and notably increased the abundance of beneficial bacteria such as Akkermansia. In conclusion, PEER exhibited a significant hypoglycemic effect in type 2 diabetic mice, providing a theoretical basis for developing <em>P. eryngii</em>-based hypoglycemic products.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"135 ","pages":"Article 107096"},"PeriodicalIF":4.0,"publicationDate":"2025-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145464268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-11-07DOI: 10.1016/j.jff.2025.107078
Simonetta Resta , Elena Bardi , Daniele D'Arrigo , Alberto Favaro , Alice Bondi , Tommaso Bonanzinga
This systematic review evaluates probiotics, paraprobiotics, and postbiotics for modulating gut microbiota along the gut–joint axis in osteoarthritis (OA) animal models. Following PRISMA guidelines and PICOS criteria, 532 articles were screened; seven met inclusion. All used rodent OA models (ACLT, PMM, DMM, MIA). Interventions included Lactobacillus acidophilus, L. pentosus, S. thermophilus, C. butyricum, heat-killed L. rhamnosus, short-chain fatty acids, gallic acid, GABA, and bile acid metabolites (UDCA, GUDCA). Treatments reshaped microbiota, enriched beneficial taxa, and restored gut barrier integrity. At the joint level, they reduced IL-1β, TNF-α, and IL-6, increased IL-10 and IL-4, mitigated pain, and promoted cartilage homeostasis by upregulating COL2, SOX9, ACAN, and downregulating MMPs and ADAMTS. None explored immune mechanisms, dose-related adverse effects, or sex-related differences. Overall, interventions consistently improved gut microbiota, inflammation, and cartilage protection, supporting therapeutic potential. However, methodological heterogeneity prevents conclusions on superiority, highlighting the need for standardized models and outcomes.
{"title":"Gut microbiota-targeted interventions for gut-joint axis modulation in experimental osteoarthritis: a systematic review of animal studies","authors":"Simonetta Resta , Elena Bardi , Daniele D'Arrigo , Alberto Favaro , Alice Bondi , Tommaso Bonanzinga","doi":"10.1016/j.jff.2025.107078","DOIUrl":"10.1016/j.jff.2025.107078","url":null,"abstract":"<div><div>This systematic review evaluates probiotics, paraprobiotics, and postbiotics for modulating gut microbiota along the gut–joint axis in osteoarthritis (OA) animal models. Following PRISMA guidelines and PICOS criteria, 532 articles were screened; seven met inclusion. All used rodent OA models (ACLT, PMM, DMM, MIA). Interventions included <em>Lactobacillus acidophilus</em>, <em>L. pentosus, S. thermophilus, C. butyricum, heat-killed L. rhamnosus</em>, short-chain fatty acids, gallic acid, GABA, and bile acid metabolites (UDCA, GUDCA). Treatments reshaped microbiota, enriched beneficial taxa, and restored gut barrier integrity. At the joint level, they reduced IL-1β, TNF-α, and IL-6, increased IL-10 and IL-4, mitigated pain, and promoted cartilage homeostasis by upregulating COL2, SOX9, ACAN, and downregulating MMPs and ADAMTS. None explored immune mechanisms, dose-related adverse effects, or sex-related differences. Overall, interventions consistently improved gut microbiota, inflammation, and cartilage protection, supporting therapeutic potential. However, methodological heterogeneity prevents conclusions on superiority, highlighting the need for standardized models and outcomes.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"135 ","pages":"Article 107078"},"PeriodicalIF":4.0,"publicationDate":"2025-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145464269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The gut–brain axis exerts critical control over neuroinflammation and neuronal resilience in traumatic brain injury (TBI) and neurodegenerative disorders. Dysregulated immune signaling, oxidative stress, and microbial dysbiosis are key pathological drivers. This review integrates current evidence on three dietary polyphenols, quercetin, allicin, and ferulic acid, that strengthen intestinal barrier integrity, remodel gut microbiota, and modulate neuroinflammatory cascades through antioxidant, anti-inflammatory, and gene-regulatory pathways. Emphasizing a mechanism-to-formulation translation, we link molecular bioactivity with the challenges of bioavailability and highlight food-grade delivery platforms such as encapsulation and microbiota-targeted systems to enhance stability, transport, and efficacy. By combining perspectives from foodomics, nutrigenomics, and neurobiology, this review proposes design principles for developing next-generation functional foods aimed at mitigating TBI- and aging-associated neurodegeneration. The work uniquely bridges nutritional biochemistry, microbiome modulation, and food processing to guide translational neuroprotective interventions.
{"title":"Dietary polyphenols and gut–brain axis modulation: the neuroprotective role of quercetin, allicin, and ferulic acid in traumatic brain injury and neurodegeneration","authors":"Ramachandran Chelliah , Sulav Ghimeray , Srinivas Mettu , Kaliyan Barathikannan , Shuai Wei , Simpy Sanyal , Manideep Pabba , Fawzi Banat , Deog-Hwan Oh","doi":"10.1016/j.jff.2025.107091","DOIUrl":"10.1016/j.jff.2025.107091","url":null,"abstract":"<div><div>The gut–brain axis exerts critical control over neuroinflammation and neuronal resilience in traumatic brain injury (TBI) and neurodegenerative disorders. Dysregulated immune signaling, oxidative stress, and microbial dysbiosis are key pathological drivers. This review integrates current evidence on three dietary polyphenols, quercetin, allicin, and ferulic acid, that strengthen intestinal barrier integrity, remodel gut microbiota, and modulate neuroinflammatory cascades through antioxidant, anti-inflammatory, and gene-regulatory pathways. Emphasizing a mechanism-to-formulation translation, we link molecular bioactivity with the challenges of bioavailability and highlight food-grade delivery platforms such as encapsulation and microbiota-targeted systems to enhance stability, transport, and efficacy. By combining perspectives from foodomics, nutrigenomics, and neurobiology, this review proposes design principles for developing next-generation functional foods aimed at mitigating TBI- and aging-associated neurodegeneration. The work uniquely bridges nutritional biochemistry, microbiome modulation, and food processing to guide translational neuroprotective interventions.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"135 ","pages":"Article 107091"},"PeriodicalIF":4.0,"publicationDate":"2025-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145464266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-11-06DOI: 10.1016/j.jff.2025.107093
Qiang Yang , Ying Cai , Zhibo Wang , Sifan Guo , Shi Qiu , Aihua Zhang
Gardenia fructus (GF), a medicinal and edible herb with over 2,000 years of use, possesses diverse bioactive compounds including iridoids, crocins, and organic acids. Herein, we summarize the ethnomedicinal applications, phytochemistry, pharmacological activities, and safety concerns. GF exhibits a wide range of pharmacological activities, notably hepatoprotective, renoprotective, and neuroprotective effects. However, dose-dependent hepatotoxicity and nephrotoxicity - primarily linked to genipin, a metabolite of geniposide - pose significant safety challenges. Mechanisms involve oxidative stress, mitochondrial dysfunction, bile acid dysregulation, and inflammatory cascade activation. The field lacks sufficient clinical translation, comprehensive bioactivity analysis, and stringent quality control. Future research should prioritize elucidating molecular toxicity pathways, developing quality control benchmarks include geniposide, and advancing clinical trials to balance efficacy with safety. This positions GF as a sustainable resource for nutraceuticals and functional foods, necessitating rigorous safety assessments for rational utilization.
{"title":"From traditional medicinal use to functional food: The pharmacological activity, hepatotoxicity mechanism and detoxification strategies of gardenia fructus","authors":"Qiang Yang , Ying Cai , Zhibo Wang , Sifan Guo , Shi Qiu , Aihua Zhang","doi":"10.1016/j.jff.2025.107093","DOIUrl":"10.1016/j.jff.2025.107093","url":null,"abstract":"<div><div>Gardenia fructus (GF), a medicinal and edible herb with over 2,000 years of use, possesses diverse bioactive compounds including iridoids, crocins, and organic acids. Herein, we summarize the ethnomedicinal applications, phytochemistry, pharmacological activities, and safety concerns. GF exhibits a wide range of pharmacological activities, notably hepatoprotective, renoprotective, and neuroprotective effects. However, dose-dependent hepatotoxicity and nephrotoxicity - primarily linked to genipin, a metabolite of geniposide - pose significant safety challenges. Mechanisms involve oxidative stress, mitochondrial dysfunction, bile acid dysregulation, and inflammatory cascade activation. The field lacks sufficient clinical translation, comprehensive bioactivity analysis, and stringent quality control. Future research should prioritize elucidating molecular toxicity pathways, developing quality control benchmarks include geniposide, and advancing clinical trials to balance efficacy with safety. This positions GF as a sustainable resource for nutraceuticals and functional foods, necessitating rigorous safety assessments for rational utilization.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"135 ","pages":"Article 107093"},"PeriodicalIF":4.0,"publicationDate":"2025-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145464265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
To further explore how Cistanche tubulosa extract (CE) alleviates alcoholic liver disease, this study examines its action through metabolic pathways beyond the established Nrf2 mechanism. Bioactive compounds in CE were first analyzed and identified using UHPLC-MS/MS. Next, an acute alcohol injury model was established, and the degree of liver injury alleviation was assessed by measuring serum biochemical indicators. Untargeted metabolomics and transcriptomics were then used to analyze the relationship between metabolites and genes, with final validation conducted through qPCR. The results showed that pretreatment with CE significantly alleviated changes in ALT, AST, TC, TG, MDA, CAT, GSH, SOD, IL-6, IL-1β, and TNF-α caused by alcohol. Multi-omics analysis revealed that CE exerts a hepatoprotective effect by regulating amino acid metabolism pathways, particularly through upregulating GOT1 expression. CE shows potential as a functional food ingredient for protecting the liver from alcohol-induced injury.
{"title":"Potential of Cistanche tubulosa extract in attenuating acute alcoholic liver injury via modulation of oxidative stress and lipid metabolism","authors":"Dilireba Shataer , Yuhang Chang , Mamateli Obul , Aikebaier Jumai , Yu Wang , Aerziguli Abulizi , Yuanyuan Hou , Haipeng Liu","doi":"10.1016/j.jff.2025.107090","DOIUrl":"10.1016/j.jff.2025.107090","url":null,"abstract":"<div><div>To further explore how <em>Cistanche tubulosa</em> extract (CE) alleviates alcoholic liver disease, this study examines its action through metabolic pathways beyond the established <em>Nrf2</em> mechanism. Bioactive compounds in CE were first analyzed and identified using UHPLC-MS/MS. Next, an acute alcohol injury model was established, and the degree of liver injury alleviation was assessed by measuring serum biochemical indicators. Untargeted metabolomics and transcriptomics were then used to analyze the relationship between metabolites and genes, with final validation conducted through qPCR. The results showed that pretreatment with CE significantly alleviated changes in ALT, AST, TC, TG, MDA, CAT, GSH, SOD, IL-6, IL-1<em>β</em>, and TNF-<em>α</em> caused by alcohol. Multi-omics analysis revealed that CE exerts a hepatoprotective effect by regulating amino acid metabolism pathways, particularly through upregulating <em>GOT1</em> expression. CE shows potential as a functional food ingredient for protecting the liver from alcohol-induced injury.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"135 ","pages":"Article 107090"},"PeriodicalIF":4.0,"publicationDate":"2025-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145464224","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Cyclodextrans (CIs) are cycloisomaltooligosaccharides that are known to function as dextran analogues and are possible inhibitors of dental plaque formation. CIs have a structure in which 7 to 12 glucose molecules are cyclically linked by α-1,6 bonds. We examined the inhibitory effects of CIs on the induction of dental caries by Streptococcus mutans. The inhibitory effects for bacterial growth, anti-enzymatic activity, and biofilm formation were analyzed. Additionally, the inhibitory effect of CIs on the induction of dental caries was investigated using a rat caries model. The presence of CIs resulted in reduced bacterial growth and biofilm formation. Kinetic analysis of the results showed that the inhibitory effect of CIs on anti-enzymatic activity is competitive. Furthermore, the caries scores with CIs were lower than those without CIs in both diet and drinking experiments. These results suggest that CIs possess strong anticaries activity and may be useful as a dietary supplement.
{"title":"Inhibitory effect of cyclodextran on the induction of dental caries by Streptococcus mutans","authors":"Haruka Asaumi, Sakuya Matsuura, Kana Goto, Daiki Matsuoka, Keiko Tabata, Shuhei Naka, Michiyo Matsumoto-Nakano","doi":"10.1016/j.jff.2025.107077","DOIUrl":"10.1016/j.jff.2025.107077","url":null,"abstract":"<div><div>Cyclodextrans (CIs) are cycloisomaltooligosaccharides that are known to function as dextran analogues and are possible inhibitors of dental plaque formation. CIs have a structure in which 7 to 12 glucose molecules are cyclically linked by α-1,6 bonds. We examined the inhibitory effects of CIs on the induction of dental caries by <em>Streptococcus mutans</em>. The inhibitory effects for bacterial growth, anti-enzymatic activity, and biofilm formation were analyzed. Additionally, the inhibitory effect of CIs on the induction of dental caries was investigated using a rat caries model. The presence of CIs resulted in reduced bacterial growth and biofilm formation. Kinetic analysis of the results showed that the inhibitory effect of CIs on anti-enzymatic activity is competitive. Furthermore, the caries scores with CIs were lower than those without CIs in both diet and drinking experiments. These results suggest that CIs possess strong anticaries activity and may be useful as a dietary supplement.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"135 ","pages":"Article 107077"},"PeriodicalIF":4.0,"publicationDate":"2025-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145464223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
扫码关注我们
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号