Pub Date : 2024-10-04DOI: 10.1016/j.jff.2024.106494
Xinyue Lin , Zongjun Li
Background
Avocado oil is an emerging health oil, which has attracted much attention in recent years due to its unique nutritional value and extensive health benefits.
Methods
This paper integrated the latest information on the nutritional composition and health benefits of avocado oil, through in-depth analysis, to clarify the rich nutrients in avocado oil and its positive effects on human health.
Objective
This paper aims to present a comprehensive and profound understanding of the framework of avocado oil.
Conclusion
Avocado oil is a nutritious cooking oil with multiple health benefits. In the future, the specific mechanism of its health benefits can be studied by multi-omics methods.
{"title":"Key components and multiple health functions of avocado oil: A review","authors":"Xinyue Lin , Zongjun Li","doi":"10.1016/j.jff.2024.106494","DOIUrl":"10.1016/j.jff.2024.106494","url":null,"abstract":"<div><h3>Background</h3><div>Avocado oil is an emerging health oil, which has attracted much attention in recent years due to its unique nutritional value and extensive health benefits.</div></div><div><h3>Methods</h3><div>This paper integrated the latest information on the nutritional composition and health benefits of avocado oil, through in-depth analysis, to clarify the rich nutrients in avocado oil and its positive effects on human health.</div></div><div><h3>Objective</h3><div>This paper aims to present a comprehensive and profound understanding of the framework of avocado oil.</div></div><div><h3>Conclusion</h3><div>Avocado oil is a nutritious cooking oil with multiple health benefits. In the future, the specific mechanism of its health benefits can be studied by multi-omics methods.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"122 ","pages":"Article 106494"},"PeriodicalIF":3.8,"publicationDate":"2024-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142426043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Peptides derived from fermented milk have demonstrated diverse bioactivities. In this study, we investigated the protective effects of Gln-Glu-Pro-Val (QEPV) against hydrogen peroxide (H2O2) induced oxidative stress in RAW 264.7 cells and further evaluated its potential in Drosophila melanogaster (D. melanogaster). Pre-incubation with QEPV in vitro dose-dependently promoted cell viability, suppressed oxidative damage, and increased antioxidant enzyme activities in a low-level oxidative stress model. QEPV remarkably extended lifespan under the low-level oxidative stress, validating its practical applicability. These effects were attributed to the upregulation of 5′ adenosine monophosphate-activated protein kinase (AMPK) and peroxisome proliferator-activated receptor alpha (PPARα) signaling pathways, as revealed by label-free proteomic analysis. To confirm the roles of these two signaling pathways, antagonists of AMPK (Dorsomorphin) and PPARα (GW6471) were applied in vitro and in vivo. The protective effects of QEPV were reversed by both antagonists. Overall, QEPV exhibits protective properties by selectively upregulating the AMPK and PPARα signaling pathways, indicating its potential application as an antioxidant food ingredient.
{"title":"Protective properties of milk-derived peptide QEPV in attenuating oxidative stress through AMPK/PPARα signaling pathways","authors":"Xiaojie Peng , Li Ma , Yuan Cheng , Guoyi Wu , Shanshan Xiao , Xingxing Zeng , Shaohui Zhang , Jiehui Zhou","doi":"10.1016/j.jff.2024.106503","DOIUrl":"10.1016/j.jff.2024.106503","url":null,"abstract":"<div><div>Peptides derived from fermented milk have demonstrated diverse bioactivities. In this study, we investigated the protective effects of Gln-Glu-Pro-Val (QEPV) against hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) induced oxidative stress in RAW 264.7 cells and further evaluated its potential in <em>Drosophila melanogaster</em> (<em>D. melanogaster</em>). Pre-incubation with QEPV <em>in vitro</em> dose-dependently promoted cell viability, suppressed oxidative damage, and increased antioxidant enzyme activities in a low-level oxidative stress model. QEPV remarkably extended lifespan under the low-level oxidative stress, validating its practical applicability. These effects were attributed to the upregulation of 5′ adenosine monophosphate-activated protein kinase (AMPK) and peroxisome proliferator-activated receptor alpha (PPARα) signaling pathways, as revealed by label-free proteomic analysis. To confirm the roles of these two signaling pathways, antagonists of AMPK (Dorsomorphin) and PPARα (GW6471) were applied <em>in vitro</em> and <em>in vivo</em>. The protective effects of QEPV were reversed by both antagonists. Overall, QEPV exhibits protective properties by selectively upregulating the AMPK and PPARα signaling pathways, indicating its potential application as an antioxidant food ingredient.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"122 ","pages":"Article 106503"},"PeriodicalIF":3.8,"publicationDate":"2024-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142426066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-04DOI: 10.1016/j.jff.2024.106492
Ayda Najmi , Negin Nikrad , Mehdi Ghaffari Sarghein , Mohammadreza Hosseinpour dogolsar , Mohammad Alizadeh
<div><h3>Context</h3><div>Ellagic acid demonstrates potential in the prevention and management of oxidative stress and inflammation markers; however, the effects of supplementation, optimal dosage, and timing are still uncertain.</div></div><div><h3>Objective</h3><div>This study aimed to assess the effects of ellagic acid supplementation on oxidative stress and inflammation markers such as malondialdehyde (MDA), total antioxidant capacity (TAC), and C-reactive protein (CRP).</div></div><div><h3>Data Sources</h3><div>A systematic search of databases (PubMed, ISI Web of Science, and Scopus) identified relevant studies until March 2024.</div></div><div><h3>Study Eligibility Criteria</h3><div>Eligible studies had to meet criteria including specific study designs (Randomized controlled clinical trials (RCT) or quasi-experimental), comparison of ellagic acid effects between control and intervention groups, and availability of data on ellagic acid’s impact on oxidative stress and inflammation markers. Outcome measures focused on markers of oxidative stress and inflammation such as MDA,TAC and CRP.</div></div><div><h3>Data Extraction</h3><div>Two independent reviewers collected information, such as specifics of ellagic acid intervention, study features, study methodology, participant attributes, intervention specifics, evaluated outcomes, and findings linked to the outcomes explored in the present study.</div></div><div><h3>Data Analysis</h3><div>Data from 5 eligible studies were analyzed, encompassing a total of 240 individuals for MDA levels, 171 participants for TAC levels, and 142 individuals for CRP levels. The results from six comparisons across five studies using a random-effects model showed that ellagic acid supplementation significantly reduced MDA levels (Weighted mean difference (WMD): −0.631 µM/L, 95 % confidence interval (CI): −1.009, −0.254; p: 0.001). Additionally, pooled data from 4 studies with 5 treatment arms indicated a significant increase in TAC levels with ellagic acid supplementation (WMD: 0.743 mg/dL, 95 % CI: 0.141, 1.345; p: 0.016). Analysis of 3 trials involving 142 individuals revealed a significant decrease in CRP levels by 3.00 ng/ml (95 % CI: −4.046, −1.954; p < 0.001) with ellagic acid supplementation. TAC levels significantly increased in individuals taking ellagic acid supplements at doses of 180 and 200 mg/day, with a meaningful enhancement in TAC in studies with an 8-week intervention but not in those lasting 12 weeks (P > 0.05). There was no significant correlation between changes in TAC levels and dosage (P, 0.06), but a significant association was found between the duration of ellagic acid supplementation. One of the basic limitations of this study is the scarcity of prior research, making the findings more of a summary and hypothesis generator for future studies.</div></div><div><h3>Conclusions</h3><div>Supplementation with ellagic acid demonstrates positive impacts on oxidative stress and inflammation when
{"title":"Ellagic acid supplementation on oxidative stress, antioxidative capacity and inflammation biomarkers: A systematic review and dose–response meta-analysis of randomized controlled trials","authors":"Ayda Najmi , Negin Nikrad , Mehdi Ghaffari Sarghein , Mohammadreza Hosseinpour dogolsar , Mohammad Alizadeh","doi":"10.1016/j.jff.2024.106492","DOIUrl":"10.1016/j.jff.2024.106492","url":null,"abstract":"<div><h3>Context</h3><div>Ellagic acid demonstrates potential in the prevention and management of oxidative stress and inflammation markers; however, the effects of supplementation, optimal dosage, and timing are still uncertain.</div></div><div><h3>Objective</h3><div>This study aimed to assess the effects of ellagic acid supplementation on oxidative stress and inflammation markers such as malondialdehyde (MDA), total antioxidant capacity (TAC), and C-reactive protein (CRP).</div></div><div><h3>Data Sources</h3><div>A systematic search of databases (PubMed, ISI Web of Science, and Scopus) identified relevant studies until March 2024.</div></div><div><h3>Study Eligibility Criteria</h3><div>Eligible studies had to meet criteria including specific study designs (Randomized controlled clinical trials (RCT) or quasi-experimental), comparison of ellagic acid effects between control and intervention groups, and availability of data on ellagic acid’s impact on oxidative stress and inflammation markers. Outcome measures focused on markers of oxidative stress and inflammation such as MDA,TAC and CRP.</div></div><div><h3>Data Extraction</h3><div>Two independent reviewers collected information, such as specifics of ellagic acid intervention, study features, study methodology, participant attributes, intervention specifics, evaluated outcomes, and findings linked to the outcomes explored in the present study.</div></div><div><h3>Data Analysis</h3><div>Data from 5 eligible studies were analyzed, encompassing a total of 240 individuals for MDA levels, 171 participants for TAC levels, and 142 individuals for CRP levels. The results from six comparisons across five studies using a random-effects model showed that ellagic acid supplementation significantly reduced MDA levels (Weighted mean difference (WMD): −0.631 µM/L, 95 % confidence interval (CI): −1.009, −0.254; p: 0.001). Additionally, pooled data from 4 studies with 5 treatment arms indicated a significant increase in TAC levels with ellagic acid supplementation (WMD: 0.743 mg/dL, 95 % CI: 0.141, 1.345; p: 0.016). Analysis of 3 trials involving 142 individuals revealed a significant decrease in CRP levels by 3.00 ng/ml (95 % CI: −4.046, −1.954; p < 0.001) with ellagic acid supplementation. TAC levels significantly increased in individuals taking ellagic acid supplements at doses of 180 and 200 mg/day, with a meaningful enhancement in TAC in studies with an 8-week intervention but not in those lasting 12 weeks (P > 0.05). There was no significant correlation between changes in TAC levels and dosage (P, 0.06), but a significant association was found between the duration of ellagic acid supplementation. One of the basic limitations of this study is the scarcity of prior research, making the findings more of a summary and hypothesis generator for future studies.</div></div><div><h3>Conclusions</h3><div>Supplementation with ellagic acid demonstrates positive impacts on oxidative stress and inflammation when","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"122 ","pages":"Article 106492"},"PeriodicalIF":3.8,"publicationDate":"2024-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142426042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-03DOI: 10.1016/j.jff.2024.106498
Biane Oliveira Philadelpho , Johnnie Elton Machado dos Santos , Emily Elaine Davis , Mariana Barros de Cerqueira e Silva , Eduardo Maffud Cilli , Ederlan de Souza Ferreira , Elvira González de Mejia
The anticancer effect of digested β-vignin and pure peptides from cowpea beans was investigated in cultivated human colon cancer cells. The effect of AQQSY on the cell cycle was similar to that of palbociclib, suggesting that AQQSY may function as a CDK-6-targeting agent. AQQSY peptide reduced the p-Rb/Rb ratio and induced apoptosis through an increase of Bax/Bcl-2 ratio, a decrease of XIAP, and activation of caspase-3. VIPASY peptide showed the potential to induce apoptosis through an increase in Bax/Bcl-2 ratio. The combination of VIPASY and AQQSY with palbociclib caused an additive effect on cell inhibition. Treatment with selected peptides from β-vignin digest caused cell cycle arrest by upregulation of p16 and apoptosis by increasing Bax/Bcl-2 ratio and caspase-8. The peptides and β-vignin digest treatments were more selective for cancer cells than the drugs. Additional research is necessary in vivo to understand the mechanisms of cowpea β-vignin-derived peptides in CRC treatment.
{"title":"Cowpea bean β-vignin-derived AQQSY peptide exerts an anticancer effect by inducing cell cycle arrest in the G0/G1 phase and modulating apoptotic signals","authors":"Biane Oliveira Philadelpho , Johnnie Elton Machado dos Santos , Emily Elaine Davis , Mariana Barros de Cerqueira e Silva , Eduardo Maffud Cilli , Ederlan de Souza Ferreira , Elvira González de Mejia","doi":"10.1016/j.jff.2024.106498","DOIUrl":"10.1016/j.jff.2024.106498","url":null,"abstract":"<div><div>The anticancer effect of digested β-vignin and pure peptides from cowpea beans was investigated in cultivated human colon cancer cells. The effect of AQQSY on the cell cycle was similar to that of palbociclib, suggesting that AQQSY may function as a CDK-6-targeting agent. AQQSY peptide reduced the p-Rb/Rb ratio and induced apoptosis through an increase of Bax/Bcl-2 ratio, a decrease of XIAP, and activation of caspase-3. VIPASY peptide showed the potential to induce apoptosis through an increase in Bax/Bcl-2 ratio. The combination of VIPASY and AQQSY with palbociclib caused an additive effect on cell inhibition. Treatment with selected peptides from β-vignin digest caused cell cycle arrest by upregulation of p16 and apoptosis by increasing Bax/Bcl-2 ratio and caspase-8. The peptides and β-vignin digest treatments were more selective for cancer cells than the drugs. Additional research is necessary in vivo to understand the mechanisms of cowpea β-vignin-derived peptides in CRC treatment.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"122 ","pages":"Article 106498"},"PeriodicalIF":3.8,"publicationDate":"2024-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142426041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-03DOI: 10.1016/j.jff.2024.106486
Nasmah K. Bastaki, Sahar A. Barhoush, Amani M. Al-Adsani
Diabetic retinopathy (DR) is a severe complication of diabetes mellitus (DM). Despite numerous studies exploring the anti-diabetic properties of garlic in managing DM symptoms, its impact on DR remains limited. This study aimed to test the effectiveness of garlic in managing DR in a streptozotocin-induced DR rat model. Rats were divided into three groups: normal control (NC), diabetic control (DC), and diabetic group (DG) treated with aqueous garlic extract (GE). Histological examination revealed a significant reduction in retinal thickness in DC rats compared to that in DG rats, which showed restored retinal thickness similar to the NC group. Immunohistochemical analyses revealed significant improvements in retinal vascular density, main artery diameter, and blood vessel numbers in DG rats compared to DC rats. The findings suggest that GE protects against DR. Furthermore, garlic could be recommended as a beneficial dietary supplement for diabetic patients to manage and delay the progression of DR.
糖尿病视网膜病变（DR）是糖尿病（DM）的一种严重并发症。尽管有许多研究探讨了大蒜在控制糖尿病症状方面的抗糖尿病特性，但其对糖尿病视网膜病变的影响仍然有限。本研究旨在测试大蒜在链脲佐菌素诱导的 DR 大鼠模型中控制 DR 的有效性。大鼠分为三组：正常对照组（NC）、糖尿病对照组（DC）和使用大蒜水提取物（GE）治疗的糖尿病组（DG）。组织学检查显示，DC 组大鼠的视网膜厚度明显减少，而 DG 组大鼠的视网膜厚度恢复得与 NC 组相似。免疫组化分析表明，与 DC 组相比，DG 组大鼠的视网膜血管密度、主动脉直径和血管数量均有明显改善。这些研究结果表明，大蒜素可预防 DR。此外，大蒜可作为一种有益的膳食补充剂推荐给糖尿病患者，以控制和延缓 DR 的进展。
{"title":"Garlic extract (Allium sativum L.) attenuates neurodegeneration and microvascular damage in rats with streptozotocin-induced diabetic retinopathy","authors":"Nasmah K. Bastaki, Sahar A. Barhoush, Amani M. Al-Adsani","doi":"10.1016/j.jff.2024.106486","DOIUrl":"10.1016/j.jff.2024.106486","url":null,"abstract":"<div><div>Diabetic retinopathy (DR) is a severe complication of diabetes mellitus (DM). Despite numerous studies exploring the anti-diabetic properties of garlic in managing DM symptoms, its impact on DR remains limited. This study aimed to test the effectiveness of garlic in managing DR in a streptozotocin-induced DR rat model. Rats were divided into three groups: normal control (NC), diabetic control (DC), and diabetic group (DG) treated with aqueous garlic extract (GE). Histological examination revealed a significant reduction in retinal thickness in DC rats compared to that in DG rats, which showed restored retinal thickness similar to the NC group. Immunohistochemical analyses revealed significant improvements in retinal vascular density, main artery diameter, and blood vessel numbers in DG rats compared to DC rats. The findings suggest that GE protects against DR. Furthermore, garlic could be recommended as a beneficial dietary supplement for diabetic patients to manage and delay the progression of DR.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"122 ","pages":"Article 106486"},"PeriodicalIF":3.8,"publicationDate":"2024-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142426064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-01DOI: 10.1016/j.jff.2024.106493
Yifei Wang, Haiyang Xu, Yan Gao, Bonian Zhao
Age-related macular degeneration (AMD) is a degenerative disease that severely impairs vision, often accompanied by oxidative stress damage, cellular apoptosis, and inflammatory responses in the retina. Green tea, which is rich in polyphenols, typically exhibits strong antioxidant, anti-apoptotic, and anti-inflammatory properties. In this study, an AMD rat model was induced through a combination of intense light exposure and intraperitoneal injection of sodium iodate. An integrated systems biology approach, combining metabolomics and transcriptomics analysis, was employed to investigate the pharmacological basis of green tea extract (GTE) intervention, elucidate key mechanisms of action, and screen for potential biomarkers. The results indicated that GTE significantly improved oxidative stress damage and inflammatory responses in the retina of AMD rats. Additionally, GTE inhibited retinal cell apoptosis by regulating the expression of caspase-3 protein. Integrated metabolomics and transcriptomics analyses revealed that GTE activates Glycerophospholipid metabolism, Ether lipid metabolism, Retinol metabolism, Arachidonic acid metabolism, and Vascular smooth muscle contraction signaling pathways in the retina of AMD rats. This activation was achieved through the modulation of gene expressions such as Pla2g3, Mboat1, and Awat2, influencing the levels of retinol, prostaglandin F2α, and multiple phospholipid metabolites in the retina. qRT-PCR experiments confirmed that GTE can regulate the expression levels of several key genes involved in inflammatory responses, phospholipid metabolism, and retinol metabolism, thereby enhancing its therapeutic activity against AMD. In summary, these findings confirm the substantial therapeutic potential of green tea in the treatment of AMD, providing a foundation for clinical applications and drug development.
{"title":"Integrated analysis of transcriptomics and metabolomics reveals the mechanisms underlying green tea intervention in age-related macular degeneration","authors":"Yifei Wang, Haiyang Xu, Yan Gao, Bonian Zhao","doi":"10.1016/j.jff.2024.106493","DOIUrl":"10.1016/j.jff.2024.106493","url":null,"abstract":"<div><div>Age-related macular degeneration (AMD) is a degenerative disease that severely impairs vision, often accompanied by oxidative stress damage, cellular apoptosis, and inflammatory responses in the retina. Green tea, which is rich in polyphenols, typically exhibits strong antioxidant, anti-apoptotic, and anti-inflammatory properties. In this study, an AMD rat model was induced through a combination of intense light exposure and intraperitoneal injection of sodium iodate. An integrated systems biology approach, combining metabolomics and transcriptomics analysis, was employed to investigate the pharmacological basis of green tea extract (GTE) intervention, elucidate key mechanisms of action, and screen for potential biomarkers. The results indicated that GTE significantly improved oxidative stress damage and inflammatory responses in the retina of AMD rats. Additionally, GTE inhibited retinal cell apoptosis by regulating the expression of caspase-3 protein. Integrated metabolomics and transcriptomics analyses revealed that GTE activates Glycerophospholipid metabolism, Ether lipid metabolism, Retinol metabolism, Arachidonic acid metabolism, and Vascular smooth muscle contraction signaling pathways in the retina of AMD rats. This activation was achieved through the modulation of gene expressions such as <em>Pla2g3</em>, <em>Mboat1</em>, and <em>Awat2</em>, influencing the levels of retinol, prostaglandin F2α, and multiple phospholipid metabolites in the retina. qRT-PCR experiments confirmed that GTE can regulate the expression levels of several key genes involved in inflammatory responses, phospholipid metabolism, and retinol metabolism, thereby enhancing its therapeutic activity against AMD. In summary, these findings confirm the substantial therapeutic potential of green tea in the treatment of AMD, providing a foundation for clinical applications and drug development.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"122 ","pages":"Article 106493"},"PeriodicalIF":3.8,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142357938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-30DOI: 10.1016/j.jff.2024.106490
Hye-Bin Lee , Yu Ra Lee , Guijae Yoo , Sangeun Yim , Hee-Kyoung Son , Choon Gil Kang , Jae Hyeok Jo , Eunjung Lee , Ho-Young Park
Interactions between the gut, adipose, and liver tissues play important roles in metabolic endotoxemia and gut dysbiosis. This study explored the effectiveness of Curcuma longa rhizome extract (CRE) in improving high-fat diet (HFD)-induced metabolic disorders and modulating gut environments. CRE treatment inhibited lipid accumulation in 3T3-L1 white adipocytes and activated thermogenesis-related genes in T37i brown adipocytes. CRE was administered to HFD-fed mice for 8 weeks, and serum, feces, colon, white adipose, and liver tissue were analyzed. CRE ameliorated the symptoms of metabolic disorders in mice with HFD-induced obesity by suppressing body weight and fat mass gain, adipocyte size, insulin resistance, and hepatic steatosis. CRE regulated gut axis-based mechanisms by inhibiting gut permeability, metabolic endotoxemia, and de novo lipogenesis, and promoting gut barrier integrity. Serum metabolites were negatively correlated with most biomarkers for metabolic disorders. Therefore, CRE could alleviate metabolic disorders by improving the intestinal environment and modulating the gut axis.
{"title":"Curcuma longa rhizome extract activates brown adipocytes and inhibits lipogenesis in high-fat diet-fed mice","authors":"Hye-Bin Lee , Yu Ra Lee , Guijae Yoo , Sangeun Yim , Hee-Kyoung Son , Choon Gil Kang , Jae Hyeok Jo , Eunjung Lee , Ho-Young Park","doi":"10.1016/j.jff.2024.106490","DOIUrl":"10.1016/j.jff.2024.106490","url":null,"abstract":"<div><div>Interactions between the gut, adipose, and liver tissues play important roles in metabolic endotoxemia and gut dysbiosis. This study explored the effectiveness of <em>Curcuma longa</em> rhizome extract (CRE) in improving high-fat diet (HFD)-induced metabolic disorders and modulating gut environments. CRE treatment inhibited lipid accumulation in 3T3-L1 white adipocytes and activated thermogenesis-related genes in T37i brown adipocytes. CRE was administered to HFD-fed mice for 8 weeks, and serum, feces, colon, white adipose, and liver tissue were analyzed. CRE ameliorated the symptoms of metabolic disorders in mice with HFD-induced obesity by suppressing body weight and fat mass gain, adipocyte size, insulin resistance, and hepatic steatosis. CRE regulated gut axis-based mechanisms by inhibiting gut permeability, metabolic endotoxemia, and de novo lipogenesis, and promoting gut barrier integrity. Serum metabolites were negatively correlated with most biomarkers for metabolic disorders. Therefore, CRE could alleviate metabolic disorders by improving the intestinal environment and modulating the gut axis.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"122 ","pages":"Article 106490"},"PeriodicalIF":3.8,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142357935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-30DOI: 10.1016/j.jff.2024.106480
M.R. Biondi Ryan , B.J. Kim , Y. Qu , D.C. Dallas
Milk-derived whey protein isolate (WPI) and glycomacropeptide (GMP) have an array of bioactivities such as antibacterial and immunomodulation. However, it is unclear whether they exert their bioactive functions beyond the gastrointestinal tract. Therefore, this study aimed to detect GMP-derived fragments in blood post-in vitro digestion and spiking and determine the presence of WPI- or GMP-derived peptides in vivo from the blood of three young adults and 15 elderly adults post-ingestion of a WPI shake. Milk-derived peptides were extracted from all plasma samples using size exclusion chromatography and solid phase extraction and analyzed using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Forty-three peptides were detected in the digested GMP-spiked plasma samples. Seventy-five milk-derived peptides were discovered in the plasma of one WPI-fed subject, many of which were homologous to bioactive sequences. No milk peptides were identified in other subjects’ plasma samples. This work provides evidence for inter-individual variation in milk-derived peptide absorption.
{"title":"Detection of milk-derived peptides in human blood post-digestion, using LC-MS/MS","authors":"M.R. Biondi Ryan , B.J. Kim , Y. Qu , D.C. Dallas","doi":"10.1016/j.jff.2024.106480","DOIUrl":"10.1016/j.jff.2024.106480","url":null,"abstract":"<div><div>Milk-derived whey protein isolate (WPI) and glycomacropeptide (GMP) have an array of bioactivities such as antibacterial and immunomodulation. However, it is unclear whether they exert their bioactive functions beyond the gastrointestinal tract. Therefore, this study aimed to detect GMP-derived fragments in blood post-in vitro digestion and spiking and determine the presence of WPI- or GMP-derived peptides in vivo from the blood of three young adults and 15 elderly adults post-ingestion of a WPI shake. Milk-derived peptides were extracted from all plasma samples using size exclusion chromatography and solid phase extraction and analyzed using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Forty-three peptides were detected in the digested GMP-spiked plasma samples. Seventy-five milk-derived peptides were discovered in the plasma of one WPI-fed subject, many of which were homologous to bioactive sequences. No milk peptides were identified in other subjects’ plasma samples. This work provides evidence for inter-individual variation in milk-derived peptide absorption.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"122 ","pages":"Article 106480"},"PeriodicalIF":3.8,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142357937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Current treatments for osteosarcoma, the most common primary bone cancer, including surgery, radiotherapy, and chemotherapy, are largely ineffective against metastatic and chemo-resistant cases, emphasizing the need for novel therapies. In this study, we investigated (20S)-Protopanaxatriol [(20S)-PPT], a natural product from Panax notoginseng, an herb used both as food and medicine, for its ability to enhance osteosarcoma sensitivity to ferroptosis induced by inducers such as RSL3, ML210, and IKE. Specifically, (20S)-PPT increased lipid peroxidation in osteosarcoma cells exposed to RSL3. This effect was mediated by ACSL4 upregulation, as ACSL4 knockdown reversed (20S)-PPT-sensitized ferroptosis. Importantly, (20S)-PPT significantly reduced tumor growth in xenograft models when combined with IKE. Collectively, our study confirms that combining (20S)-PPT with ferroptosis inducers offers a promising novel strategy for treating osteosarcoma.
骨肉瘤是最常见的原发性骨癌，目前的治疗方法包括手术、放疗和化疗，但对转移性和化疗耐药病例基本无效，因此需要新型疗法。在这项研究中，我们研究了(20S)-Protopanaxatriol [(20S)-PPT]--一种来自三七的天然产物，三七是一种既可食用又可药用的草药--是否能增强骨肉瘤对由RSL3、ML210和IKE等诱导剂诱导的铁变态反应的敏感性。具体来说，(20S)-PPT 增加了暴露于 RSL3 的骨肉瘤细胞的脂质过氧化反应。这种效应是由ACSL4上调介导的，因为ACSL4敲除可逆转（20S）-PPT敏化的铁变态反应。重要的是，(20S)-PPT 与 IKE 联用可显著降低异种移植模型中的肿瘤生长。总之，我们的研究证实，将（20S）-PPT 与铁突变诱导剂结合使用，为治疗骨肉瘤提供了一种前景广阔的新策略。
{"title":"A natural protopanaxatriol from Panax notoginseng enhances osteosarcoma sensitivity to ferroptosis via ASCL4 upregulation","authors":"Zhuo Chen, Renhua Ni, Yuanyu Hu, Yiyuan Yang, Yun Tian","doi":"10.1016/j.jff.2024.106488","DOIUrl":"10.1016/j.jff.2024.106488","url":null,"abstract":"<div><div>Current treatments for osteosarcoma, the most common primary bone cancer, including surgery, radiotherapy, and chemotherapy, are largely ineffective against metastatic and chemo-resistant cases, emphasizing the need for novel therapies. In this study, we investigated (20S)-Protopanaxatriol [(20S)-PPT], a natural product from Panax notoginseng, an herb used both as food and medicine, for its ability to enhance osteosarcoma sensitivity to ferroptosis induced by inducers such as RSL3, ML210, and IKE. Specifically, (20S)-PPT increased lipid peroxidation in osteosarcoma cells exposed to RSL3. This effect was mediated by ACSL4 upregulation, as ACSL4 knockdown reversed (20S)-PPT-sensitized ferroptosis. Importantly, (20S)-PPT significantly reduced tumor growth in xenograft models when combined with IKE. Collectively, our study confirms that combining (20S)-PPT with ferroptosis inducers offers a promising novel strategy for treating osteosarcoma.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"122 ","pages":"Article 106488"},"PeriodicalIF":3.8,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142357936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-29DOI: 10.1016/j.jff.2024.106481
Yifan Chen , Jie Zhou , Xiaoyang Liu , Lijun You
Alcoholic liver disease is one of the main causes of global liver disease-related deaths, which has emerged as a major health concern for people. In this study, a peptide was prepared from Pinctada Martensii meat (named HPM-3), and its protective effect against alcohol-induced liver damage in mice was investigated. The results indicated that intervention of HPM-3 significantly decreased the levels of transaminase and lactate dehydrogenase in serum, and improved the antioxidant enzyme activities in the liver. Besides, HPM-3 could alleviate alcohol-induced liver injury by mediating Keap1/Nrf2 signaling pathway. Moreover, HPM-3 could preserve the diversity of the gut microbiota, and restore the relative abundance of some microbial communities that were impacted by alcohol intervention. HPM-3 intervention also affected the secretion of L-glutamine and other associated metabolites and mediated the related metabolic pathways. The results would help to clarify the mechanisms of protective effects of marine peptides against alcohol-induced liver injury.
{"title":"The mechanisms of protective effect of a peptide from Pinctada martensii meat against alcohol-induced liver injury in mice","authors":"Yifan Chen , Jie Zhou , Xiaoyang Liu , Lijun You","doi":"10.1016/j.jff.2024.106481","DOIUrl":"10.1016/j.jff.2024.106481","url":null,"abstract":"<div><div>Alcoholic liver disease is one of the main causes of global liver disease-related deaths, which has emerged as a major health concern for people. In this study, a peptide was prepared from <em>Pinctada Martensii</em> meat (named HPM-3), and its protective effect against alcohol-induced liver damage in mice was investigated. The results indicated that intervention of HPM-3 significantly decreased the levels of transaminase and lactate dehydrogenase in serum, and improved the antioxidant enzyme activities in the liver. Besides, HPM-3 could alleviate alcohol-induced liver injury by mediating Keap1/Nrf2 signaling pathway. Moreover, HPM-3 could preserve the diversity of the gut microbiota, and restore the relative abundance of some microbial communities that were impacted by alcohol intervention. HPM-3 intervention also affected the secretion of L-glutamine and other associated metabolites and mediated the related metabolic pathways. The results would help to clarify the mechanisms of protective effects of marine peptides against alcohol-induced liver injury.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"122 ","pages":"Article 106481"},"PeriodicalIF":3.8,"publicationDate":"2024-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142357934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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