Pub Date : 2025-01-01DOI: 10.1016/j.jff.2024.106637
Kaiyue Liu , Ke Zhang , Yang Yang , Ying Zong , Zhongmei He , Weijia Chen , Jianming Li , Rui Du
The medicinal use of pilose antler was first described in Shennong's Classic of Materia Medica for treating kidney yang deficiency. Therefore, in this study, sika deer antler protein (SDAP) was used as a component analysis and its impacts on the immunomodulatory effects of RAW264.7 cells and kidney yang deficiency mice, as well as the intestinal flora of mice, were investigated. The results showed that SDAP could regulate the secretion of associated factors and the expression of genes and proteins of NF-κB-related signaling pathway in RAW264.7 cells induced by lipopolysaccharide and kidney yang deficiency mice. Additionally, SDAP could regulate intestinal homeostasis by increasing the abundance of beneficial bacteria Lactobacillus and Clostridium, reducing the abundance of pathogenic bacteria Proteobacteria, and maintaining some bacterial homeostasis in kidney-yang deficiency mice, so as to decrease intestinal edema, effectively improve intestinal microecology, and improve the immune function of the organism.
{"title":"Effects of sika deer antler protein on immune regulation and intestinal microbiota in mice","authors":"Kaiyue Liu , Ke Zhang , Yang Yang , Ying Zong , Zhongmei He , Weijia Chen , Jianming Li , Rui Du","doi":"10.1016/j.jff.2024.106637","DOIUrl":"10.1016/j.jff.2024.106637","url":null,"abstract":"<div><div>The medicinal use of pilose antler was first described in Shennong's Classic of Materia Medica for treating kidney yang deficiency. Therefore, in this study, sika deer antler protein (SDAP) was used as a component analysis and its impacts on the immunomodulatory effects of RAW264.7 cells and kidney yang deficiency mice, as well as the intestinal flora of mice, were investigated. The results showed that SDAP could regulate the secretion of associated factors and the expression of genes and proteins of NF-κB-related signaling pathway in RAW264.7 cells induced by lipopolysaccharide and kidney yang deficiency mice. Additionally, SDAP could regulate intestinal homeostasis by increasing the abundance of beneficial bacteria Lactobacillus and Clostridium, reducing the abundance of pathogenic bacteria Proteobacteria, and maintaining some bacterial homeostasis in kidney-yang deficiency mice, so as to decrease intestinal edema, effectively improve intestinal microecology, and improve the immune function of the organism.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"124 ","pages":"Article 106637"},"PeriodicalIF":3.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143160142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-01DOI: 10.1016/j.jff.2024.106626
Abdulrahman H. Laftah , Nawfal Alhelfi , Sadeq K. Al Salait , Ammar B. Altemimi , Mohammad Reza Tabandeh , Efstathia Tsakali , Jan F.M. Van Impe , Ahmed A. Abd El-Maksoud , Tarek Gamal Abedelmaksoud
This study evaluated the effect of green tea and Moringa oleifera extracts (at 1 % and 2 % concentration) on DOX-treated breast cancer mice. 36 Balb/C female mice with breast cancer were split into healthy mice, 4 T1 cell cancer-induced, and Doxorubicin-induced malignancies. Assessments included weight, tumor size, liver enzymes, antioxidant enzymes, oxidative stress markers, pro-inflammatory cytokines, gene expressions for apoptosis and inflammation (BAX, BCL2, NLRP3, NFKB), and liver tissues were histopathologically examined. DOX increased liver enzymes and may have damaged the liver. The hepatoprotective effects of these extracts, notably at 2 %, were apparent in their enzyme reduction. Liver CAT, GPX, and SOD activity increased and TOS and OSI levels decreased due to the extracts. Additionally, the herbal treatment lowered pro-inflammatory cytokine levels and regulated apoptosis-related gene expression, lowering BAX and increasing BCL2, promoting cell survival, and reducing inflammation. Herbal extracts can reduce NLRP3/NFKB expression in DOX-treated mice's livers, depending on dosage. Histopathological evaluation showed that highly-dose therapy reduced hepatocyte degradation, inflammatory cell infiltration, localized necrosis, and blood vessel congestion. Finally, green tea and Moringa oleifera extracts protect against DOX-induced hepatotoxicity.
{"title":"Mitigation of doxorubicin-induced liver toxicity in mice breast cancer model by green tea and Moringa oleifera combination: Targeting apoptosis, inflammation, and oxidative stress","authors":"Abdulrahman H. Laftah , Nawfal Alhelfi , Sadeq K. Al Salait , Ammar B. Altemimi , Mohammad Reza Tabandeh , Efstathia Tsakali , Jan F.M. Van Impe , Ahmed A. Abd El-Maksoud , Tarek Gamal Abedelmaksoud","doi":"10.1016/j.jff.2024.106626","DOIUrl":"10.1016/j.jff.2024.106626","url":null,"abstract":"<div><div>This study evaluated the effect of green tea and <em>Moringa oleifera</em> extracts (at 1 % and 2 % concentration) on DOX-treated breast cancer mice. 36 Balb/C female mice with breast cancer were split into healthy mice, 4 T1 cell cancer-induced, and Doxorubicin-induced malignancies. Assessments included weight, tumor size, liver enzymes, antioxidant enzymes, oxidative stress markers, pro-inflammatory cytokines, gene expressions for apoptosis and inflammation (BAX, BCL2, NLRP3, NFKB), and liver tissues were histopathologically examined. DOX increased liver enzymes and may have damaged the liver. The hepatoprotective effects of these extracts, notably at 2 %, were apparent in their enzyme reduction. Liver CAT, GPX, and SOD activity increased and TOS and OSI levels decreased due to the extracts. Additionally, the herbal treatment lowered pro-inflammatory cytokine levels and regulated apoptosis-related gene expression, lowering <strong>BAX</strong> and increasing BCL2, promoting cell survival, and reducing inflammation. Herbal extracts can reduce NLRP3/NFKB expression in DOX-treated mice's livers, depending on dosage. Histopathological evaluation showed that highly-dose therapy reduced hepatocyte degradation, inflammatory cell infiltration, localized necrosis, and blood vessel congestion. Finally, green tea and <em>Moringa oleifera</em> extracts protect against DOX-induced hepatotoxicity.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"124 ","pages":"Article 106626"},"PeriodicalIF":3.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143160173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sustainability in the fruit industry and protection of local cultivars are nowadays important tasks for consumers and authorities. Phenolic compounds are interesting to explore because they play an important role in fruit-related health benefits. The phenolic profile of the regional and commercial Spanish apple cultivars was performed by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). For a deeper understanding of their anti-inflammatory and antioxidant potential, the 5-lipoxigenase inhibition and NO− scavenging were evaluated. Moreover, cellular viability was determined to perform MTT assay in HepG2 and HeLa cell lines to discard cytotoxicity. The results showed that procyanidin B2 (PB2), 3-caffeoylquinic acid, epicatechin and 4-hydroxy benzoic acid were the most abundant phenolic compounds. Autochthonous apple cultivars showed better 5-LOX/NO− inhibition and nontoxic effects in the tested cell lines with an interesting profile of phenolic compounds. Local autochthonous apples can be considered a better source of bioactive compounds than the commercial samples.
{"title":"Valorization of local regional apple (Malus domestica Borkh.) cultivars versus commercial samples from Spain: Phenolic compounds by HPLC-MS/MS, cytotoxicity and biological potential on nitric oxide radicals and lipoxygenase inhibition","authors":"Adrián Millán-Laleona , Pilar Cebollada , Giovanni Caprioli , Diletta Piatti , Filippo Maggi , Ana Pina , Carlota Gómez-Rincón , Víctor López","doi":"10.1016/j.jff.2024.106631","DOIUrl":"10.1016/j.jff.2024.106631","url":null,"abstract":"<div><div>Sustainability in the fruit industry and protection of local cultivars are nowadays important tasks for consumers and authorities. Phenolic compounds are interesting to explore because they play an important role in fruit-related health benefits. The phenolic profile of the regional and commercial Spanish apple cultivars was performed by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). For a deeper understanding of their anti-inflammatory and antioxidant potential, the 5-lipoxigenase inhibition and NO<sup>−</sup> scavenging were evaluated. Moreover, cellular viability was determined to perform MTT assay in HepG2 and HeLa cell lines to discard cytotoxicity. The results showed that procyanidin B2 (PB2), 3-caffeoylquinic acid, epicatechin and 4-hydroxy benzoic acid were the most abundant phenolic compounds. Autochthonous apple cultivars showed better 5-LOX/NO<sup>−</sup> inhibition and nontoxic effects in the tested cell lines with an interesting profile of phenolic compounds. Local autochthonous apples can be considered a better source of bioactive compounds than the commercial samples.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"124 ","pages":"Article 106631"},"PeriodicalIF":3.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143160231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-01DOI: 10.1016/j.jff.2024.106651
Xiujuan Li , Lun Liu , Song Wei
Gynostemma pentaphyllum (GP), recognized as both an edible vegetable and health tea, has been consumed for thousands of years. Chemical research indicates GP primarily contains active ingredients including saponins, polysaccharides, and flavonoids. Notably, saponins and polysaccharides are among the main active components of GP, which have been extensively utilized in medicine, food, healthcare, and cosmetics due to their bioactivities including anti-tumor, anti-oxidation, hypoglycemic, cardioprotective, neuroprotective and anti-inflammatory activities. We conducted searches in various online databases, including Elsevier, Springer, PubMed, Web of Science, and China Knowledge Network (CNKI), to gather literature on the traditional uses, structural characteristics, and biological activities of the active ingredients of GP published prior to October 2024. In this review, GP saponins were categorized and GP polysaccharides discovered in the last ten years were highlighted. Then, the bioactivities of GP are summarized and lays a solid foundation for its development as a functional health food.
{"title":"Gynostemma pentaphyllum: A review on its traditional uses, phytochemistry and pharmacology","authors":"Xiujuan Li , Lun Liu , Song Wei","doi":"10.1016/j.jff.2024.106651","DOIUrl":"10.1016/j.jff.2024.106651","url":null,"abstract":"<div><div><em>Gynostemma pentaphyllum</em> (GP), recognized as both an edible vegetable and health tea, has been consumed for thousands of years. Chemical research indicates GP primarily contains active ingredients including saponins, polysaccharides, and flavonoids. Notably, saponins and polysaccharides are among the main active components of GP, which have been extensively utilized in medicine, food, healthcare, and cosmetics due to their bioactivities including anti-tumor, anti-oxidation, hypoglycemic, cardioprotective, neuroprotective and anti-inflammatory activities. We conducted searches in various online databases, including Elsevier, Springer, PubMed, Web of Science, and China Knowledge Network (CNKI), to gather literature on the traditional uses, structural characteristics, and biological activities of the active ingredients of GP published prior to October 2024. In this review, GP saponins were categorized and GP polysaccharides discovered in the last ten years were highlighted. Then, the bioactivities of GP are summarized and lays a solid foundation for its development as a functional health food.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"124 ","pages":"Article 106651"},"PeriodicalIF":3.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143159501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-01DOI: 10.1016/j.jff.2024.106628
Ji Gu , Yongchao Wang , Shanshan Xiao , Yunye Zheng , Zhengxuan Wang , Yaping Liu , Limeng Zhang , Guiguang Cheng
Nicotiana tabacum L. seed oil is rich in unsaturated fatty acids and has potential applications in functional or nutritional foods. The present study aimed to investigate the protective effects of Nicotiana tabacum L. seed oil (NC89 and BS4) against D-galactose (D-gal) -induced brain damage in aging mice and the potential molecular mechanisms. Combined with GC/MS component analysis and network pharmacology prediction, the aging related PI3K/Akt/Nrf2 pathway was found for further verification. Histopathological results showed that NC89 and BS4 could significantly improve the cellular damage of liver, brain, kidney and heart tissues in D-gal-induced aging mice. NC89 and BS4 exerted antioxidant capacity by increasing the activities of SOD, CAT, GSH-Px and T-AOC in serum, liver and brain tissues, and reducing the accumulation of MDA in plasma. In addition, NC89 and BS4 also reduced the contents of serum TNF-α and IL-6, showing an anti-inflammatory effect. Notably, NC89 and BS4 protect cranial nerves by increasing AChe activity in the brain. Western blotting results showed that NC89 and BS4 increased the ratios of p-PI3K/PI3K and p-Akt/Akt, as well as the protein expression of Nrf2, NQO1 and HO-1 in brain tissues. Therefore, the potential protective mechanism of NC89 and BS4 on the liver and brain may be related to the activation of the PI3K/Akt/Nrf2 pathway and the inhibition of inflammatory responses. This study provides new insights into the biological activities of NC89 and BS4.
{"title":"Protective effects of seed oil from Nicotiana tabacum L. On D-galactose induced liver and brain damages of mice by regulating PI3K/Akt/Nrf2 signaling pathway","authors":"Ji Gu , Yongchao Wang , Shanshan Xiao , Yunye Zheng , Zhengxuan Wang , Yaping Liu , Limeng Zhang , Guiguang Cheng","doi":"10.1016/j.jff.2024.106628","DOIUrl":"10.1016/j.jff.2024.106628","url":null,"abstract":"<div><div><em>Nicotiana tabacum</em> L. seed oil is rich in unsaturated fatty acids and has potential applications in functional or nutritional foods. The present study aimed to investigate the protective effects of <em>Nicotiana tabacum</em> L. seed oil (NC89 and BS4) against D-galactose (D-gal) -induced brain damage in aging mice and the potential molecular mechanisms. Combined with GC/MS component analysis and network pharmacology prediction, the aging related PI3K/Akt/Nrf2 pathway was found for further verification. Histopathological results showed that NC89 and BS4 could significantly improve the cellular damage of liver, brain, kidney and heart tissues in D-gal-induced aging mice. NC89 and BS4 exerted antioxidant capacity by increasing the activities of SOD, CAT, GSH-Px and T-AOC in serum, liver and brain tissues, and reducing the accumulation of MDA in plasma. In addition, NC89 and BS4 also reduced the contents of serum TNF-α and IL-6, showing an anti-inflammatory effect. Notably, NC89 and BS4 protect cranial nerves by increasing AChe activity in the brain. Western blotting results showed that NC89 and BS4 increased the ratios of p-PI3K/PI3K and p-Akt/Akt, as well as the protein expression of Nrf2, NQO1 and HO-1 in brain tissues. Therefore, the potential protective mechanism of NC89 and BS4 on the liver and brain may be related to the activation of the PI3K/Akt/Nrf2 pathway and the inhibition of inflammatory responses. This study provides new insights into the biological activities of NC89 and BS4.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"124 ","pages":"Article 106628"},"PeriodicalIF":3.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143159502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-01DOI: 10.1016/j.jff.2024.106654
Shi Qiu , Sifan Guo , Zhibo Wang , Ying Cai , Dandan Xie , Wanying Sun , Aihua Zhang
Metabolic abnormalities lead to the dysfunction of metabolic pathways and metabolite accumulation or deficiency which is well-recognized hallmarks of diseases. Unique metabolic needs of cells highlight the potential for fruitful drug and target discovery. Astragaloside IV (ASIV) is a representative component of traditional herbs Astragalus membranaceus which belongs to medicinal food homology, but the molecular targets regulating cellular amino acid (AA) metabolism of the therapeutic effect are still unclear. Here, we integrated metabolomics and proteomics approach with pattern recognition to exploring potential targets and AA metabolic characteristics of ASIV on diabetic db/db mouse and Min6 cell lines. Intracellular metabolites and the dysregulated proteins were detected and analyzed by liquid chromatography/mass spectrometry/mass spectrometry and parallel reaction monitoring assays. According to the correlation matrix of the dysregulated metabolites/proteins interactions, we found that Pdxk was ranked as top target signature that was closely related to AA metabolism. Mechanistically, ASIV inhibits Pdxk by suppression of the vitamin B6 pathway, thereby ameliorating AA metabolism disorders. Intriguingly, ASIV affected the metabolite production of the vitamin B6 metabolism, especially had a significantly downregulated expression of 4-pyridoxic acid and pyridoxal 5′-phosphate, leading to the suppression of AA metabolism pathway. It also provides evidence that integrated metabolomics and proteomics approach reveal ASIV inhibits Pdxk as a potential therapeutic target by regulating AA metabolic homeostasis for diabetes treatment, and laying a new paradigm for diabetes treatment. In short, the integrated metabolomics and proteomics approach could reveal ASIV inhibits Pdxk as a potential target by regulating AA metabolic homeostasis and laying a new paradigm for precision treatment of future diseases.
{"title":"Integrated metabolomics and proteomics revealed astragaloside IV inhibiting Pdxk-mediated vitamin B6 metabolism based on liquid chromatography-tandem mass spectrometry combined with pattern recognition and correlation analysis","authors":"Shi Qiu , Sifan Guo , Zhibo Wang , Ying Cai , Dandan Xie , Wanying Sun , Aihua Zhang","doi":"10.1016/j.jff.2024.106654","DOIUrl":"10.1016/j.jff.2024.106654","url":null,"abstract":"<div><div>Metabolic abnormalities lead to the dysfunction of metabolic pathways and metabolite accumulation or deficiency which is well-recognized hallmarks of diseases. Unique metabolic needs of cells highlight the potential for fruitful drug and target discovery. Astragaloside IV (ASIV) is a representative component of traditional herbs <em>Astragalus membranaceus</em> which belongs to medicinal food homology, but the molecular targets regulating cellular amino acid (AA) metabolism of the therapeutic effect are still unclear. Here, we integrated metabolomics and proteomics approach with pattern recognition to exploring potential targets and AA metabolic characteristics of ASIV on diabetic db/db mouse and Min6 cell lines. Intracellular metabolites and the dysregulated proteins were detected and analyzed by liquid chromatography/mass spectrometry/mass spectrometry and parallel reaction monitoring assays. According to the correlation matrix of the dysregulated metabolites/proteins interactions, we found that Pdxk was ranked as top target signature that was closely related to AA metabolism. Mechanistically, ASIV inhibits Pdxk by suppression of the vitamin B6 pathway, thereby ameliorating AA metabolism disorders. Intriguingly, ASIV affected the metabolite production of the vitamin B6 metabolism, especially had a significantly downregulated expression of 4-pyridoxic acid and pyridoxal 5′-phosphate, leading to the suppression of AA metabolism pathway. It also provides evidence that integrated metabolomics and proteomics approach reveal ASIV inhibits Pdxk as a potential therapeutic target by regulating AA metabolic homeostasis for diabetes treatment, and laying a new paradigm for diabetes treatment. In short, the integrated metabolomics and proteomics approach could reveal ASIV inhibits Pdxk as a potential target by regulating AA metabolic homeostasis and laying a new paradigm for precision treatment of future diseases.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"124 ","pages":"Article 106654"},"PeriodicalIF":3.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143160775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-01DOI: 10.1016/j.jff.2024.106623
Jiahao Ying , Shijie Li , Anna Ying , Yindong Ding , Xiaoyin Zhu , Jiahao Lin , Kehe Yu
Exploring the therapeutic effects and underlying mechanisms of Apigenin (Api) in managing osteoarthritis (OA) was the primary focus of this research. In this research, X-ray and Safranin-O staining were employed to evaluate the imageology and histomorphology differences in the knee joints of mice in vivo. Techniques such as Western blot analysis, Enzyme-linked Immunosorbent Assay (ELISA), and immunofluorescence staining were applied to examine protein level changes in vitro experiments. In vitro assays indicated that Api significantly reduces the levels of pro-inflammatory mediators and prevents the degradation of the ECM. By promoting SIRT1/mTOR pathway-mediated mitophagy, Api shields chondrocytes from pyroptosis. Further, animal studies demonstrated that Api effectively alleviates the progression of OA by enhancing mitophagy, inhibiting chondrocyte pyroptosis and ECM degradation. Consequently, Api is recognized as an effective and promising therapeutic candidate for the management and treatment of OA.
{"title":"Apigenin suppresses osteoarthritis progression by inhibiting pyroptosis via SIRT1/mTOR pathway-mediated mitophagy","authors":"Jiahao Ying , Shijie Li , Anna Ying , Yindong Ding , Xiaoyin Zhu , Jiahao Lin , Kehe Yu","doi":"10.1016/j.jff.2024.106623","DOIUrl":"10.1016/j.jff.2024.106623","url":null,"abstract":"<div><div>Exploring the therapeutic effects and underlying mechanisms of Apigenin (Api) in managing osteoarthritis (OA) was the primary focus of this research. In this research, X-ray and Safranin-O staining were employed to evaluate the imageology and histomorphology differences in the knee joints of mice in vivo. Techniques such as Western blot analysis, Enzyme-linked Immunosorbent Assay (ELISA), and immunofluorescence staining were applied to examine protein level changes in vitro experiments. In vitro assays indicated that Api significantly reduces the levels of pro-inflammatory mediators and prevents the degradation of the ECM. By promoting SIRT1/mTOR pathway-mediated mitophagy, Api shields chondrocytes from pyroptosis. Further, animal studies demonstrated that Api effectively alleviates the progression of OA by enhancing mitophagy, inhibiting chondrocyte pyroptosis and ECM degradation. Consequently, Api is recognized as an effective and promising therapeutic candidate for the management and treatment of OA.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"124 ","pages":"Article 106623"},"PeriodicalIF":3.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143160235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-01DOI: 10.1016/j.jff.2024.106608
Dawei Cheng , Xinyu Huang , Caihong Zhang , Ben Shao , Xueling Li , Meiqing Li
The aim of the present study was to examine the antihypertensive effect of black soybean peptides (BSP) and investigate the role of these peptides in ameliorating hypertension-induced intestinal damage and modulating gut microbiota. The findings demonstrated that BSP could significantly reduce the blood pressure of mice with diet-induced hypertension (P < 0.001). Simultaneously, BSP effectively inhibited the production of pro-inflammatory factors (IL-17 and TNF-α) and increased the levels of an anti-inflammatory factor (IL-10) in the colon of mouse models of hypertension. Additionally, BSP up-regulated the gene expression of intestinal barrier factors ZO-1, Occludin, and MUC-2, and alleviated the hypertension-induced inflammatory infiltration of colon tissue. Furthermore, the peptides increased the levels of short-chain fatty acids (SCFAs) in the feces and up-regulated the protein expression of the SCFA receptor GPR41/43. The analysis of intestinal microbes showed that BSP could modulate the richness and diversity of the intestinal microbiota in mouse models of hypertension, increase the abundance of beneficial bacteria, decrease the abundance of harmful bacteria, and promote the homeostasis of the imbalanced intestinal microbiota. Notably, BSP exerted a significant protective effect against L-NAME-induced hypertension in mice, demonstrating good outcomes with respect to blood pressure regulation, intestinal protection, and intestinal microbiota balance. Therefore, BSP may ameliorate the harm caused by hypertension and could aid in the prevention of hypertension as a dietary intervention.
{"title":"Impact of black soybean peptides on intestinal barrier function and gut microbiota in hypertensive mice","authors":"Dawei Cheng , Xinyu Huang , Caihong Zhang , Ben Shao , Xueling Li , Meiqing Li","doi":"10.1016/j.jff.2024.106608","DOIUrl":"10.1016/j.jff.2024.106608","url":null,"abstract":"<div><div>The aim of the present study was to examine the antihypertensive effect of black soybean peptides (BSP) and investigate the role of these peptides in ameliorating hypertension-induced intestinal damage and modulating gut microbiota. The findings demonstrated that BSP could significantly reduce the blood pressure of mice with diet-induced hypertension (<em>P</em> < 0.001). Simultaneously, BSP effectively inhibited the production of pro-inflammatory factors (IL-17 and TNF-α) and increased the levels of an anti-inflammatory factor (IL-10) in the colon of mouse models of hypertension. Additionally, BSP up-regulated the gene expression of intestinal barrier factors <em>ZO-1</em>, <em>Occludin</em>, and <em>MUC-2</em>, and alleviated the hypertension-induced inflammatory infiltration of colon tissue. Furthermore, the peptides increased the levels of short-chain fatty acids (SCFAs) in the feces and up-regulated the protein expression of the SCFA receptor GPR41/43. The analysis of intestinal microbes showed that BSP could modulate the richness and diversity of the intestinal microbiota in mouse models of hypertension, increase the abundance of beneficial bacteria, decrease the abundance of harmful bacteria, and promote the homeostasis of the imbalanced intestinal microbiota. Notably, BSP exerted a significant protective effect against L-NAME-induced hypertension in mice, demonstrating good outcomes with respect to blood pressure regulation, intestinal protection, and intestinal microbiota balance. Therefore, BSP may ameliorate the harm caused by hypertension and could aid in the prevention of hypertension as a dietary intervention.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"123 ","pages":"Article 106608"},"PeriodicalIF":3.8,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142745877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-01DOI: 10.1016/j.jff.2024.106613
Yao Ge , Wenhui Ye , Yue Wang , Aiqin Ma , Man Wang , Qingshan Chen , Xiaoyan Huo , Chunxi Liu , Yujie Shi , Biao Liu , Wei Chen
This study investigated the effects of a nutritional milk composition containing red ginseng extract (MCCRG) on the skin barrier function and immunity of middle-aged women. Fifty women (aged, 25 to 45) consumed MCCRG orally for 56 days. Evaluations of skin phenotype, facial bacterial microbiome, immune system function, and clinical diagnoses were conducted. The results indicated that MCCRG significantly improved skin hydration, firmness, elasticity, and glossiness. Furthermore, MCCRG also reduced TEWL and wrinkles and enhanced skin barrier integrity. Additionally, high IgG levels and improved CD4+/CD8+ ratios due to MCCRG consumption increased facial microbiota diversity and immune function. Clinical diagnoses provided supplementary evidence for the efficacy of MCCRG. The findings of this study demonstrated that MCCRG, through nutritional support, enhanced both skin barrier and immune system functions. This study offers substantial evidence of the innovative therapeutic effects of nutritional support on skin barrier damage and immune function.
{"title":"Effects of milk powder fortified with red ginseng extracts on the skin barrier function and immunity of middle-aged women","authors":"Yao Ge , Wenhui Ye , Yue Wang , Aiqin Ma , Man Wang , Qingshan Chen , Xiaoyan Huo , Chunxi Liu , Yujie Shi , Biao Liu , Wei Chen","doi":"10.1016/j.jff.2024.106613","DOIUrl":"10.1016/j.jff.2024.106613","url":null,"abstract":"<div><div>This study investigated the effects of a nutritional milk composition containing red ginseng extract (MCCRG) on the skin barrier function and immunity of middle-aged women. Fifty women (aged, 25 to 45) consumed MCCRG orally for 56 days. Evaluations of skin phenotype, facial bacterial microbiome, immune system function, and clinical diagnoses were conducted. The results indicated that MCCRG significantly improved skin hydration, firmness, elasticity, and glossiness. Furthermore, MCCRG also reduced TEWL and wrinkles and enhanced skin barrier integrity. Additionally, high IgG levels and improved CD4+/CD8+ ratios due to MCCRG consumption increased facial microbiota diversity and immune function. Clinical diagnoses provided supplementary evidence for the efficacy of MCCRG. The findings of this study demonstrated that MCCRG, through nutritional support, enhanced both skin barrier and immune system functions. This study offers substantial evidence of the innovative therapeutic effects of nutritional support on skin barrier damage and immune function.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"123 ","pages":"Article 106613"},"PeriodicalIF":3.8,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142746203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-01DOI: 10.1016/j.jff.2024.106625
Zhipeng Zheng , Ruoting Hou , Shaoling Lin , Natthida Sriboonvorakul , Jiamiao Hu
Up-to-date, studies have underscored pivotal roles of dietary interventions in modulating immune function. However, a comprehensive bibliometric analysis and synthesis of their findings seems to be absent. To fill this knowledge gap, the relevant publications from 2002 to 2024 were screened out and analyzed using bibliometric quantitative methods. The results illustrate China led in publication volume (76 publications) over the past two decades, with Ocean University of China and Changhu Xue being leading contributors; while Food & Function featured the highest number of articles in this field. Furthermore, the analysis also identified the following research trends, including development of research models and methodologies for evaluating immunity enhancement, increasing number of bioactivities with identified immunoenhancing properties, and the exploration of their underlying mechanisms. Taken together, this comprehensive bibliometric analysis may delineate the thematic trends in this area and offer useful references for identifying novel dietary approaches to augment immunity in future.
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