首页 > 最新文献

BDJ Open最新文献

英文 中文
Detection of viable oral bacteria of the patient on the surgical mask of dentists. 在牙医的外科口罩上检测病人口腔中的存活细菌。
IF 3 Q2 Dentistry Pub Date : 2024-01-16 DOI: 10.1038/s41405-023-00182-4
Madline Priska Gund, Jusef Naim, Janina Lang, Matthias Hannig, Barbara Gärtner, Alexander Halfmann, Gabor Boros, Stefan Rupf

Introduction and aim: Bioaerosols contaminate the personal protective equipment (PPE), especially masks. The PPE harbors microorganisms from various sources. However, no previous studies have investigated the specific sources of bacteria found on used masks and their correlation with those from the treated patient.

Setting, design, material and methods: Intraoral samples from the patient were collected prior to dental aerosol-producing treatments using a nylon flock fiber swab. After treatment, the practitioner's mask was imprinted onto agar plates.

Main outcome methods: Following cultivation, colony forming units were counted and identified using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). After the samples were analyzed, the intraoral samples as well as the mask samples were assessed for the presence of identical species, which were subsequently quantified.

Results: 126 treatments were included. One species match occurred most frequently (26.2%), followed by two (11.9%%) and three or more (3.97%). In the intraoral samples, Neisseria subflava occurred most often, within mask samples Staphylococcus epidermidis were detected most. Staphylococcus aureus could be cultivated three times more often in intraoral samples than on the mask.

Discussion and conclusion: Oral microorganisms originating from the patient's oral cavity can be found on the outside of masks. When using PPE during treatments, it should therefore always be in mind that potentially pathogenic microorganisms may land on the mask becoming a source of for itself.

简介和目的:生物气溶胶会污染个人防护设备(PPE),尤其是口罩。个人防护设备上的微生物来源多种多样。然而,以前没有研究调查过在使用过的口罩上发现的细菌的具体来源及其与接受治疗的患者口罩上细菌的相关性:在牙科气溶胶治疗前,使用尼龙纤维拭子收集患者口腔内的样本。治疗后,将医生的口罩印在琼脂平板上:培养后,使用基质辅助激光解吸/电离飞行时间质谱法(MALDI-TOF MS)对菌落形成单位进行计数和鉴定。分析样本后,评估口腔内样本和口罩样本中是否存在相同菌种,随后对其进行量化:结果:共纳入 126 种治疗方法。最常见的是一个物种匹配(26.2%),其次是两个(11.9%)和三个或三个以上(3.97%)。在口腔内样本中,最常出现的是亚弗拉伐奈瑟菌,而在口罩样本中,表皮葡萄球菌的检出率最高。口腔内样本中金黄色葡萄球菌的培养率是口罩上金黄色葡萄球菌培养率的三倍:讨论和结论:口罩外部可以发现来自患者口腔的口腔微生物。因此,在治疗过程中使用个人防护设备时,应始终牢记潜在的致病微生物可能会落在口罩上,成为口罩本身的病源。
{"title":"Detection of viable oral bacteria of the patient on the surgical mask of dentists.","authors":"Madline Priska Gund, Jusef Naim, Janina Lang, Matthias Hannig, Barbara Gärtner, Alexander Halfmann, Gabor Boros, Stefan Rupf","doi":"10.1038/s41405-023-00182-4","DOIUrl":"10.1038/s41405-023-00182-4","url":null,"abstract":"<p><strong>Introduction and aim: </strong>Bioaerosols contaminate the personal protective equipment (PPE), especially masks. The PPE harbors microorganisms from various sources. However, no previous studies have investigated the specific sources of bacteria found on used masks and their correlation with those from the treated patient.</p><p><strong>Setting, design, material and methods: </strong>Intraoral samples from the patient were collected prior to dental aerosol-producing treatments using a nylon flock fiber swab. After treatment, the practitioner's mask was imprinted onto agar plates.</p><p><strong>Main outcome methods: </strong>Following cultivation, colony forming units were counted and identified using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). After the samples were analyzed, the intraoral samples as well as the mask samples were assessed for the presence of identical species, which were subsequently quantified.</p><p><strong>Results: </strong>126 treatments were included. One species match occurred most frequently (26.2%), followed by two (11.9%%) and three or more (3.97%). In the intraoral samples, Neisseria subflava occurred most often, within mask samples Staphylococcus epidermidis were detected most. Staphylococcus aureus could be cultivated three times more often in intraoral samples than on the mask.</p><p><strong>Discussion and conclusion: </strong>Oral microorganisms originating from the patient's oral cavity can be found on the outside of masks. When using PPE during treatments, it should therefore always be in mind that potentially pathogenic microorganisms may land on the mask becoming a source of for itself.</p>","PeriodicalId":36997,"journal":{"name":"BDJ Open","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10791691/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139479422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Distress and well-being in dentists: performance of a screening tool for assessment. 牙医的压力和幸福感:筛查评估工具的性能。
IF 3 Q2 Dentistry Pub Date : 2024-01-16 DOI: 10.1038/s41405-024-00185-9
Subha Giri, Colin P West, Tait Shanafelt, Daniel Satele, Liselotte N Dyrbye

Objectives: Dentists' well-being is being challenged today by many factors. However, effective screening tools to assess their distress and well-being are yet to be validated. The present study aims to evaluate the ability of the Well-Being Index (WBI) to identify distress and stratify dentists' well-being and their likelihood for adverse professional consequences.

Method and materials: A convenience sample of dentists completed a web-based 9-item WBI survey along with other instruments that measured quality of life (QOL), fatigue, burnout, and questions about suicidal ideation, recent dental error, and intent to leave their current job.

Results: A total of 597 dentists completed the survey. The overall mean WBI score was 2.3. The mean WBI score was significantly greater in dentists with low QOL than among dentists without low QOL (4.1 vs 1.6, p < 0.001). Dentists with extreme fatigue, burnout, and suicidal ideation had significantly higher mean WBI score than those without distress (all p < 0.001). WBI score stratified the dentists' likelihood of reporting a recent dental error and intent to leave their current job.

Conclusion: The WBI may be a useful screening tool to assess well-being among dentists and identify those in distress and at risk for adverse professional consequences.

目的:如今,牙科医生的幸福感正受到多种因素的挑战。然而,评估他们的困扰和幸福感的有效筛查工具尚未得到验证。本研究旨在评估幸福指数(WBI)识别困扰的能力,并对牙医的幸福感及其职业不良后果的可能性进行分层:方便抽样的牙科医生完成了一项基于网络的 9 项 WBI 调查,以及其他测量生活质量 (QOL)、疲劳、职业倦怠的工具,还有关于自杀意念、近期牙科错误和离职意向的问题:共有 597 名牙医完成了调查。WBI总平均分为2.3分。低质量生活水平牙医的 WBI 平均得分明显高于无低质量生活水平的牙医(4.1 vs 1.6,P 结论:WBI 可作为一种有用的筛查工具:WBI 可能是一种有用的筛查工具,可用于评估牙医的健康状况,并识别处于困境和面临不良职业后果风险的牙医。
{"title":"Distress and well-being in dentists: performance of a screening tool for assessment.","authors":"Subha Giri, Colin P West, Tait Shanafelt, Daniel Satele, Liselotte N Dyrbye","doi":"10.1038/s41405-024-00185-9","DOIUrl":"10.1038/s41405-024-00185-9","url":null,"abstract":"<p><strong>Objectives: </strong>Dentists' well-being is being challenged today by many factors. However, effective screening tools to assess their distress and well-being are yet to be validated. The present study aims to evaluate the ability of the Well-Being Index (WBI) to identify distress and stratify dentists' well-being and their likelihood for adverse professional consequences.</p><p><strong>Method and materials: </strong>A convenience sample of dentists completed a web-based 9-item WBI survey along with other instruments that measured quality of life (QOL), fatigue, burnout, and questions about suicidal ideation, recent dental error, and intent to leave their current job.</p><p><strong>Results: </strong>A total of 597 dentists completed the survey. The overall mean WBI score was 2.3. The mean WBI score was significantly greater in dentists with low QOL than among dentists without low QOL (4.1 vs 1.6, p < 0.001). Dentists with extreme fatigue, burnout, and suicidal ideation had significantly higher mean WBI score than those without distress (all p < 0.001). WBI score stratified the dentists' likelihood of reporting a recent dental error and intent to leave their current job.</p><p><strong>Conclusion: </strong>The WBI may be a useful screening tool to assess well-being among dentists and identify those in distress and at risk for adverse professional consequences.</p>","PeriodicalId":36997,"journal":{"name":"BDJ Open","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10791682/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139479423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The efficacy of various irrigation techniques on the removal of double antibiotic paste from simulated immature roots and the amount of apically extruded debris. 各种灌洗技术对去除模拟未成熟牙根上的双抗生素糊剂和根尖挤出碎屑量的效果。
IF 3 Q2 Dentistry Pub Date : 2024-01-05 DOI: 10.1038/s41405-023-00183-3
Shaimaa Nasr El-Din, Reham Hassan

Objective: This study evaluated the effect of the XP-Endo Finisher (XPF), passive ultrasonic irrigation (PUI) and conventional irrigation using side-vented needle (SVN) on the amount of apically extruded debris and canal cleanliness following the removal of double antibiotic paste (DAP) from immature root canal models.

Material and methods: Forty-eight extracted mandibular premolars were drilled using peeso drills to simulate immature apices. The canals were filled with DAP and were randomly assigned into 3 groups according to the DAP removal method: XPF, PUI, and SVN (n = 16). The amount of extruded debris was assessed with an analytical balance then roots were split longitudinally and imaged using stereomicroscope to evaluate the residual medicament. Data were statistically analyzed using Kruskal-Wallis and Dunn's test. Spearman's correlation coefficient was used to determine significant correlation between extruded debris and the residual DAP scores.

Results: There was no significant difference between debris extrusion values for all groups (P value 0.237). For canal cleanliness, the amount of remaining DAP was significantly lower in the XPF and PUI compared to SVN (P value < 0.001). A non-significant positive (direct) correlation was found between the amounts of apically extruded debris and residual DAP scores (P value 0.087).

Conclusion: XPF and PUI were associated with better canal cleanliness during removal of DAP, no difference could be found between the three irrigation techniques regarding the debris extrusion.

研究目的本研究评估了 XP-Endo Finisher (XPF)、被动超声波冲洗 (PUI) 和使用侧进针 (SVN) 的传统冲洗对未成熟根管模型去除双抗生素糊剂 (DAP) 后根尖挤出碎屑量和根管清洁度的影响:使用peeso钻模拟未成熟根尖,对48颗拔出的下颌前磨牙进行钻孔。用 DAP 填充根管,并根据去除 DAP 的方法随机分为 3 组:XPF组、PUI组和SVN组(n = 16)。用分析天平评估挤出的碎屑量,然后纵向劈开牙根,用立体显微镜成像以评估残留药物。数据采用 Kruskal-Wallis 和 Dunn 检验进行统计分析。斯皮尔曼相关系数用于确定挤出碎屑与残留 DAP 评分之间的显著相关性:各组的碎屑挤出量无明显差异(P 值为 0.237)。在牙道清洁度方面,XPF 和 PUI 组的残余 DAP 量明显低于 SVN 组(P 值 结论:XPF 和 PUI 组的残余 DAP 量明显高于 SVN 组(P 值):在去除 DAP 的过程中,XPF 和 PUI 的牙道清洁度更好,但三种灌洗技术在碎屑挤出方面没有差异。
{"title":"The efficacy of various irrigation techniques on the removal of double antibiotic paste from simulated immature roots and the amount of apically extruded debris.","authors":"Shaimaa Nasr El-Din, Reham Hassan","doi":"10.1038/s41405-023-00183-3","DOIUrl":"10.1038/s41405-023-00183-3","url":null,"abstract":"<p><strong>Objective: </strong>This study evaluated the effect of the XP-Endo Finisher (XPF), passive ultrasonic irrigation (PUI) and conventional irrigation using side-vented needle (SVN) on the amount of apically extruded debris and canal cleanliness following the removal of double antibiotic paste (DAP) from immature root canal models.</p><p><strong>Material and methods: </strong>Forty-eight extracted mandibular premolars were drilled using peeso drills to simulate immature apices. The canals were filled with DAP and were randomly assigned into 3 groups according to the DAP removal method: XPF, PUI, and SVN (n = 16). The amount of extruded debris was assessed with an analytical balance then roots were split longitudinally and imaged using stereomicroscope to evaluate the residual medicament. Data were statistically analyzed using Kruskal-Wallis and Dunn's test. Spearman's correlation coefficient was used to determine significant correlation between extruded debris and the residual DAP scores.</p><p><strong>Results: </strong>There was no significant difference between debris extrusion values for all groups (P value 0.237). For canal cleanliness, the amount of remaining DAP was significantly lower in the XPF and PUI compared to SVN (P value < 0.001). A non-significant positive (direct) correlation was found between the amounts of apically extruded debris and residual DAP scores (P value 0.087).</p><p><strong>Conclusion: </strong>XPF and PUI were associated with better canal cleanliness during removal of DAP, no difference could be found between the three irrigation techniques regarding the debris extrusion.</p>","PeriodicalId":36997,"journal":{"name":"BDJ Open","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10770069/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139106769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Shear bond strength of a RMGIC for orthodontic bracket bonding to enamel. 用于正畸托槽与珐琅质粘接的 RMGIC 的剪切粘接强度。
IF 3 Q2 Dentistry Pub Date : 2024-01-02 DOI: 10.1038/s41405-023-00181-5
Maureen Boudrot, Philippe François, Sarah Abdel-Gawad, Jean-Pierre Attal, Claire-Adeline Dantagnan

Objective: To evaluate the shear bond strength (SBS) of a restorative resin-modified glass ionomer cement (RMGIC) for orthodontic bracket bonding.

Materials and methods: One hundred twenty-one human teeth were randomly divided into 11 groups (n = 11) according to the surface treatment applied (H3PO4 ± Transbond Plus (TSEP) or Scotchbond Universal (SU)), and the adhesive used (Riva LC HV (RIVA), Fuji Ortho (FUJI), and Transbond XT (TXT)). For each sample, a metal button was bonded. SBS tests were performed at 1 week and debonded specimens were observed for failure modes determination. One-way ANOVA followed by Tukey's post hoc test was used to compare SBS differences and Fisher's exact test to analyze the failure modes (p < 0.05).

Results: TSEP + FUJI and H3PO4 + SU + TXT showed the highest SBS values while H3PO4 + TSEP + RIVA showed the lowest value. Cohesive failure and mixed failure were found in the groups with SU and TXT and adhesive failure in the other groups.

Discussion/conclusions: The bonding of orthodontic attachments to enamel could be performed with any of the three materials studied. The use of a universal adhesive in the bonding protocol could optimize the adhesion values. Clinical studies would be needed to confirm the results obtained.

摘要评估用于正畸托槽粘接的修复用树脂改性玻璃离子粘结剂(RMGIC)的剪切粘结强度(SBS):根据表面处理(H3PO4 ± Transbond Plus (TSEP) 或 Scotchbond Universal (SU))和使用的粘合剂(Riva LC HV (RIVA)、Fuji Ortho (FUJI) 和 Transbond XT (TXT)),将 121 颗人类牙齿随机分为 11 组(n = 11)。每个样品都粘接了一个金属钮扣。1 周后进行 SBS 测试,观察脱粘试样以确定失效模式。采用单因子方差分析和 Tukey 后检验比较 SBS 差异,采用费雪精确检验分析失效模式(P 结果:TSEP + FUJI 和 H3PO4 + SU + TXT 的 SBS 值最高,而 H3PO4 + TSEP + RIVA 的 SBS 值最低。在含有 SU 和 TXT 的组别中发现了内聚失效和混合失效,而在其他组别中发现了粘接失效:正畸附着体与牙釉质的粘接可以使用所研究的三种材料中的任何一种。在粘接方案中使用通用粘合剂可以优化粘接值。需要进行临床研究来证实所获得的结果。
{"title":"Shear bond strength of a RMGIC for orthodontic bracket bonding to enamel.","authors":"Maureen Boudrot, Philippe François, Sarah Abdel-Gawad, Jean-Pierre Attal, Claire-Adeline Dantagnan","doi":"10.1038/s41405-023-00181-5","DOIUrl":"10.1038/s41405-023-00181-5","url":null,"abstract":"<p><strong>Objective: </strong>To evaluate the shear bond strength (SBS) of a restorative resin-modified glass ionomer cement (RMGIC) for orthodontic bracket bonding.</p><p><strong>Materials and methods: </strong>One hundred twenty-one human teeth were randomly divided into 11 groups (n = 11) according to the surface treatment applied (H<sub>3</sub>PO<sub>4</sub> ± Transbond Plus (TSEP) or Scotchbond Universal (SU)), and the adhesive used (Riva LC HV (RIVA), Fuji Ortho (FUJI), and Transbond XT (TXT)). For each sample, a metal button was bonded. SBS tests were performed at 1 week and debonded specimens were observed for failure modes determination. One-way ANOVA followed by Tukey's post hoc test was used to compare SBS differences and Fisher's exact test to analyze the failure modes (p < 0.05).</p><p><strong>Results: </strong>TSEP + FUJI and H<sub>3</sub>PO<sub>4</sub> + SU + TXT showed the highest SBS values while H<sub>3</sub>PO<sub>4</sub> + TSEP + RIVA showed the lowest value. Cohesive failure and mixed failure were found in the groups with SU and TXT and adhesive failure in the other groups.</p><p><strong>Discussion/conclusions: </strong>The bonding of orthodontic attachments to enamel could be performed with any of the three materials studied. The use of a universal adhesive in the bonding protocol could optimize the adhesion values. Clinical studies would be needed to confirm the results obtained.</p>","PeriodicalId":36997,"journal":{"name":"BDJ Open","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10762087/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139088922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
In vitro shear bond strength over zirconia and titanium alloy and degree of conversion of extraoral compared to intraoral self-adhesive resin cements 与口内自粘树脂水门汀相比,口外自粘树脂水门汀对氧化锆和钛合金的体外剪切粘接强度以及转化程度
IF 3 Q2 Dentistry Pub Date : 2023-12-09 DOI: 10.1038/s41405-023-00178-0
Vincent Fouquet, Claire-Adeline Dantagnan, Sarah Abdel-Gawad, Elisabeth Dursun, Jean-Pierre Attal, Philippe François

Objective

Evaluation of the Shear bond strength over zirconia and titanium alloy and degree of conversion of extraoral compared to intraoral self-adhesive resin cements.

Materials and methods

Nine bonding protocols were carried out on zirconia 4Y-TZP and titanium alloy (Ti-6Al-4V). Seven resin cement (one extraoral and six intraoral) were tested in the shear bond strength test and the degree of conversion measurements.

Results

The significantly highest value was obtained for Monobond Plus + Multilink Hybrid Abutment, the extraoral resin cement for both titanium alloy (35.1 MPa) and zirconia (32.9 MPa). For each resin, significantly higher DC values were obtained for the dual-cure mode compared with the self-cure mode. Regardless of the cure mode, Nexus Universal reached the highest DC (78.4%).

Discussion/Conclusions

In this study, the extraoral self-curing resin cement showed the higher bond strength values on zirconia and titanium alloy when associated with a universal primer. Some intraoral dual-cure resin cements showed closed performances when used with universal primers. There is no direct correlation between the degree of conversion of the resin cement and the shear bond strength obtained on the prosthetic materials tested.

材料和方法在氧化锆 4Y-TZP 和钛合金(Ti-6Al-4V)上进行了九种粘接试验。结果Monobond Plus + Multilink Hybrid基台是口外树脂粘结剂,对钛合金(35.1 MPa)和氧化锆(32.9 MPa)的粘结力值明显最高。对于每种树脂,双固化模式的 DC 值都明显高于自固化模式。讨论/结论 在这项研究中,口外自固化树脂水门汀与通用底漆配合使用时,在氧化锆和钛合金上显示出更高的粘结强度值。一些口内双固化树脂水门汀在与通用底漆一起使用时表现出封闭性能。在测试的修复材料上,树脂水门汀的转化程度与获得的剪切粘接强度之间没有直接关系。
{"title":"In vitro shear bond strength over zirconia and titanium alloy and degree of conversion of extraoral compared to intraoral self-adhesive resin cements","authors":"Vincent Fouquet, Claire-Adeline Dantagnan, Sarah Abdel-Gawad, Elisabeth Dursun, Jean-Pierre Attal, Philippe François","doi":"10.1038/s41405-023-00178-0","DOIUrl":"https://doi.org/10.1038/s41405-023-00178-0","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Objective</h3><p>Evaluation of the Shear bond strength over zirconia and titanium alloy and degree of conversion of extraoral compared to intraoral self-adhesive resin cements.</p><h3 data-test=\"abstract-sub-heading\">Materials and methods</h3><p>Nine bonding protocols were carried out on zirconia 4Y-TZP and titanium alloy (Ti-6Al-4V). Seven resin cement (one extraoral and six intraoral) were tested in the shear bond strength test and the degree of conversion measurements.</p><h3 data-test=\"abstract-sub-heading\">Results</h3><p>The significantly highest value was obtained for Monobond Plus + Multilink Hybrid Abutment, the extraoral resin cement for both titanium alloy (35.1 MPa) and zirconia (32.9 MPa). For each resin, significantly higher DC values were obtained for the dual-cure mode compared with the self-cure mode. Regardless of the cure mode, Nexus Universal reached the highest DC (78.4%).</p><h3 data-test=\"abstract-sub-heading\">Discussion/Conclusions</h3><p>In this study, the extraoral self-curing resin cement showed the higher bond strength values on zirconia and titanium alloy when associated with a universal primer. Some intraoral dual-cure resin cements showed closed performances when used with universal primers. There is no direct correlation between the degree of conversion of the resin cement and the shear bond strength obtained on the prosthetic materials tested.</p>","PeriodicalId":36997,"journal":{"name":"BDJ Open","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2023-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138567652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Mechanical and chemical surface treatment enhances bond strength between zirconia and orthodontic brackets: an in vitro study 机械和化学表面处理可增强氧化锆与正畸托槽之间的粘接强度:体外研究
IF 3 Q2 Dentistry Pub Date : 2023-12-08 DOI: 10.1038/s41405-023-00180-6
Nareudee Limpuangthip, Atikom Surintanasarn, Ploylada Vitavaspan

Objectives

To assess the shear bond strength (SBS) between metal orthodontic brackets and zirconia after receiving different mechanical and chemical surface treatments, and different types of resin adhesive. The failure mode of each treatment protocol was also evaluated.

Materials and methods

The present in vitro experimental study consisted of six surface treatment protocols with two different resin adhesives. One-hundred and forty-four rectangular-shaped 3 mol% yttrium-stabilized tetragonal zirconia polycrystal blocks were milled, sintered, and embedded in acrylic resin. They were randomly divided into three mechanical (none, air abrasion, and bur grinding) and two chemical surface treatment conditions (no primer and Z-primer). The specimens were divided into two groups according to the resin adhesive received: self-cured (RelyX U200) and light-cured adhesives (Transbond XT). The SBS between the metal bracket and zirconia was tested using a universal testing machine (1-mm/min crosshead speed), and the failure mode was evaluated. Differences in SBS and failure mode were analyzed using Welch ANOVA followed by post-hoc comparison and Fisher’s Exact test, respectively.

Results

Bur grinding produced the highest SBS, followed by air abrasion. Z-primer application typically provided a higher SBS regardless of resin adhesive used (p < 0.001). Without primer application, RelyX U200 provided a higher SBS than Transbond XT (p < 0.001). After grinding, using Z-primer and RelyX U200 resulted in a higher SBS than no primer and using Transbond XT (p < 0.001). Adhesive failure at the zirconia–adhesive interface occurred only when Transbond XT was applied without bur grinding, and when using Transbond XT after grinding, but no Z-primer application.

Conclusion

Bur grinding combined with applying an MDP-containing primer and resin adhesive enhances the SBS between zirconia and metal orthodontic brackets.

目的 评估金属正畸托槽和氧化锆在接受不同的机械和化学表面处理以及不同类型的树脂粘合剂后的剪切粘接强度(SBS)。材料和方法本体外实验研究包括六种表面处理方案和两种不同的树脂粘合剂。将 144 块 3 mol% 钇稳定四角氧化锆多晶体矩形块研磨、烧结并嵌入丙烯酸树脂中。这些试样被随机分为三种机械处理条件(无、气磨和毛刺研磨)和两种化学表面处理条件(无底漆和 Z-底漆)。试样根据所使用的树脂粘合剂分为两组:自固化粘合剂(RelyX U200)和光固化粘合剂(Transbond XT)。使用万能试验机(十字头速度为 1 毫米/分钟)测试金属支架和氧化锆之间的 SBS,并评估失效模式。采用韦尔奇方差分析(Welch ANOVA)和费雪精确检验(Fisher's Exact Test)分别分析了 SBS 和失效模式的差异。无论使用哪种树脂粘合剂,使用 Z 型底漆通常都能产生更高的 SBS(p <0.001)。在不使用底胶的情况下,RelyX U200 的 SBS 比 Transbond XT 高(p < 0.001)。打磨后,使用 Z-primer 和 RelyX U200 的 SBS 要高于不使用底胶和使用 Transbond XT 的 SBS(p < 0.001)。只有在使用 Transbond XT 时未进行毛刺研磨,以及在研磨后使用 Transbond XT 但未使用 Z-primer 时,氧化锆-粘合剂界面才会发生粘合失效。
{"title":"Mechanical and chemical surface treatment enhances bond strength between zirconia and orthodontic brackets: an in vitro study","authors":"Nareudee Limpuangthip, Atikom Surintanasarn, Ploylada Vitavaspan","doi":"10.1038/s41405-023-00180-6","DOIUrl":"https://doi.org/10.1038/s41405-023-00180-6","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Objectives</h3><p>To assess the shear bond strength (SBS) between metal orthodontic brackets and zirconia after receiving different mechanical and chemical surface treatments, and different types of resin adhesive. The failure mode of each treatment protocol was also evaluated.</p><h3 data-test=\"abstract-sub-heading\">Materials and methods</h3><p>The present in vitro experimental study consisted of six surface treatment protocols with two different resin adhesives. One-hundred and forty-four rectangular-shaped 3 mol% yttrium-stabilized tetragonal zirconia polycrystal blocks were milled, sintered, and embedded in acrylic resin. They were randomly divided into three mechanical (none, air abrasion, and bur grinding) and two chemical surface treatment conditions (no primer and Z-primer). The specimens were divided into two groups according to the resin adhesive received: self-cured (RelyX U200) and light-cured adhesives (Transbond XT). The SBS between the metal bracket and zirconia was tested using a universal testing machine (1-mm/min crosshead speed), and the failure mode was evaluated. Differences in SBS and failure mode were analyzed using Welch ANOVA followed by post-hoc comparison and Fisher’s Exact test, respectively.</p><h3 data-test=\"abstract-sub-heading\">Results</h3><p>Bur grinding produced the highest SBS, followed by air abrasion. Z-primer application typically provided a higher SBS regardless of resin adhesive used (<i>p</i> &lt; 0.001). Without primer application, RelyX U200 provided a higher SBS than Transbond XT (<i>p</i> &lt; 0.001). After grinding, using Z-primer and RelyX U200 resulted in a higher SBS than no primer and using Transbond XT (<i>p</i> &lt; 0.001). Adhesive failure at the zirconia–adhesive interface occurred only when Transbond XT was applied without bur grinding, and when using Transbond XT after grinding, but no Z-primer application.</p><h3 data-test=\"abstract-sub-heading\">Conclusion</h3><p>Bur grinding combined with applying an MDP-containing primer and resin adhesive enhances the SBS between zirconia and metal orthodontic brackets.</p>","PeriodicalId":36997,"journal":{"name":"BDJ Open","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2023-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138553080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Reconciling duty: a theory and typology of professionalism. 调和责任:专业主义的理论与类型。
IF 3 Q2 Dentistry Pub Date : 2023-12-04 DOI: 10.1038/s41405-023-00172-6
Andrew Trathen, Sasha Scambler, Jennifer E Gallagher

Background: Professionalism is expected of health professionals and advocated by professional regulators in the United Kingdom (UK). Concepts of professionalism have evolved in sociological discourse and its meaning for dentistry is unclear. It is, none-the-less, considered a core domain of dental education and professional practice by the United Kingdom regulator, the General Dental Council. This paper reports the sense-making process, or social process, of professionalism in practice within England.

Aim: To explore the research question 'What does dental professionalism mean in practice?

Methods: Taking a constructivist grounded theory approach, involving purposive and theoretical sampling, 24 dental professionals were recruited to participate in this qualitative study. In-depth, semi-structured interviews were conducted by one interviewer (AT). Interviews were recorded, transcribed verbatim, and analysed leading to the development of a theory grounded in the data.

Results: A focus on the social-professional constructs used by participants to make sense of their experiences, resulted in a grounded theory where Reconciling Duty emerged as the core category. This represents a process of meeting professional duties to different parties that are often mutually exclusive. It is comprised of three supporting categories: Applying order to the system, where individuals attempt to identify what constitutes professional attitudes and behaviours, Rationalising what is fair, where individuals make judgements on how the conflict between duties should be resolved, and finally Responding to the System, where individuals attempt to actualise these desired resolutions in the context of the complex social system in which they practice. Three dentist archetypes (typologies) emerged, which involved a personal (Type 1), patient (Type 2), or a societal (Type 3) compromise.

Conclusion: Professionalism can be conceptualised as process of reconciling multiple, competing, legitimate duties to different parties, in seeking a fair solution. Once this has been identified, individuals need to work within the complex system of dentistry to make their identified outcome a reality. The findings suggest that using the theory of Reconciling Duty helps us to engage with the meaning that the participants drew from the term 'professionalism', and anchors it in the lived, everyday professional experiences and challenges faced. A novel typology is proposed, commensurate with calls for a systems approach to the topic.

背景:专业精神是对卫生专业人员的期望,也是英国专业监管机构所提倡的。专业主义的概念已经在社会学话语中发展,其对牙科的意义尚不清楚。尽管如此,它还是被英国监管机构牙科总委员会认为是牙科教育和专业实践的核心领域。本文报道了专业主义在英国实践中的意义形成过程或社会过程。目的:探讨“牙科专业精神在实践中的意义”这一研究问题。方法:采用建构主义扎根理论方法,采用目的性抽样和理论抽样的方法,对24名牙科专业人员进行定性研究。深度、半结构化访谈由一位采访者(AT)进行。采访被记录下来,逐字抄录,并进行分析,从而形成基于数据的理论。结果:对参与者用来理解他们的经历的社会专业结构的关注,产生了一个扎根的理论,其中调和责任成为核心类别。这代表了一个履行专业职责的过程,而这些职责往往是相互排斥的。它由三个支持类别组成:将秩序应用于系统,个人试图确定什么构成专业态度和行为;合理化什么是公平的,个人对如何解决职责之间的冲突做出判断;最后对系统做出反应,个人试图在他们实践的复杂社会系统中实现这些期望的解决方案。出现了三种牙医原型(类型学),涉及个人(类型1),患者(类型2)或社会(类型3)妥协。结论:职业化可以被定义为在寻求公平解决方案的过程中,协调对不同当事人的多种、相互竞争的、合法的责任。一旦确定了这一点,个人需要在复杂的牙科系统内工作,使他们确定的结果成为现实。研究结果表明,使用调和责任理论有助于我们理解参与者从“专业”一词中汲取的含义,并将其锚定在生活中,日常的专业经验和面临的挑战中。提出了一种新的类型学,与对该主题的系统方法的要求相称。
{"title":"Reconciling duty: a theory and typology of professionalism.","authors":"Andrew Trathen, Sasha Scambler, Jennifer E Gallagher","doi":"10.1038/s41405-023-00172-6","DOIUrl":"10.1038/s41405-023-00172-6","url":null,"abstract":"<p><strong>Background: </strong>Professionalism is expected of health professionals and advocated by professional regulators in the United Kingdom (UK). Concepts of professionalism have evolved in sociological discourse and its meaning for dentistry is unclear. It is, none-the-less, considered a core domain of dental education and professional practice by the United Kingdom regulator, the General Dental Council. This paper reports the sense-making process, or social process, of professionalism in practice within England.</p><p><strong>Aim: </strong>To explore the research question 'What does dental professionalism mean in practice?</p><p><strong>Methods: </strong>Taking a constructivist grounded theory approach, involving purposive and theoretical sampling, 24 dental professionals were recruited to participate in this qualitative study. In-depth, semi-structured interviews were conducted by one interviewer (AT). Interviews were recorded, transcribed verbatim, and analysed leading to the development of a theory grounded in the data.</p><p><strong>Results: </strong>A focus on the social-professional constructs used by participants to make sense of their experiences, resulted in a grounded theory where Reconciling Duty emerged as the core category. This represents a process of meeting professional duties to different parties that are often mutually exclusive. It is comprised of three supporting categories: Applying order to the system, where individuals attempt to identify what constitutes professional attitudes and behaviours, Rationalising what is fair, where individuals make judgements on how the conflict between duties should be resolved, and finally Responding to the System, where individuals attempt to actualise these desired resolutions in the context of the complex social system in which they practice. Three dentist archetypes (typologies) emerged, which involved a personal (Type 1), patient (Type 2), or a societal (Type 3) compromise.</p><p><strong>Conclusion: </strong>Professionalism can be conceptualised as process of reconciling multiple, competing, legitimate duties to different parties, in seeking a fair solution. Once this has been identified, individuals need to work within the complex system of dentistry to make their identified outcome a reality. The findings suggest that using the theory of Reconciling Duty helps us to engage with the meaning that the participants drew from the term 'professionalism', and anchors it in the lived, everyday professional experiences and challenges faced. A novel typology is proposed, commensurate with calls for a systems approach to the topic.</p>","PeriodicalId":36997,"journal":{"name":"BDJ Open","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2023-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10696065/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138483184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Influence of endodontic motors on the behaviour of root canal shaping instruments: an in vitro comparative study. 根管马达对根管成形器械的影响:一项体外比较研究。
IF 3 Q2 Dentistry Pub Date : 2023-11-29 DOI: 10.1038/s41405-023-00179-z
Clémentine Reynette, Renaud Giess, Jeanne Davril, Jean-Marc Martrette, Éric Mortier, Rémy Balthazard, Marin Vincent

Aims: The endodontic literature reports a lot of comparative study on endodontic instruments, concerning as well their geometry, instrumental dynamics, material, mechanical behavior or heat treatment. However, to our knowledge, no study has focused on the influence of endodontic motors on the shaping abilities of endodontic instruments. Thus, the aim of this study was to analyze the influence of the endodontic motors on root canal shaping instruments.

Method: Dual Move (MICRO-MEGA, Besançon, France), Canal Pro CL2i (COLTENE, Alstätten, Suisse), Canal Pro Jeni Motor (COLTENE, Alstätten, Suisse), Ai Motor (WOODPECKER, Guilin, China), Wave One motor (VDW, Postfach, Munich) and Smart A (WOODPECKER, Guilin, China) were pre-clinically compared in continuous rotation and reciprocating motion on a traction/compression bench using resin blocks. Canal shaping in continuous rotation and reciprocating motion were performed with One Curve and One RECI instruments (MICRO-MEGA, Besançon, France), respectively. The penetration/removal forces, making it possible to objectify the cutting effect and screwing effect of the instruments during root canal shaping, were analyzed.

Results: The results showed (i) that endodontic motors influence the mechanical behavior of endodontic instruments, (ii) that the influence of the motors is essentially felt during reciprocating motion and (iii) that the reciprocating angles influence the mechanical behavior of endodontic instruments.

Conclusion: Only endodontic instruments are widely studied in literature while endodontic motors have a direct influence on root canal treatment. This study analyzes the influence of the endodontic motors on root canal shaping instruments. This study tends to demonstrate that Jeni Motor could optimize the mechanical behavior of endodontic instruments.

目的:牙髓学文献报道了大量的牙髓器械的比较研究,包括它们的几何形状、仪器动力学、材料、力学行为或热处理。然而,据我们所知,还没有研究集中在牙髓马达对牙髓器械成型能力的影响。因此,本研究的目的是分析根管马达对根管成形器械的影响。方法:Dual Move (MICRO-MEGA, besanon,法国)、Canal Pro CL2i (COLTENE, Alstätten,瑞士)、Canal Pro Jeni Motor (COLTENE, Alstätten,瑞士)、Ai Motor (WOODPECKER,桂林,中国)、Wave One Motor (VDW, Postfach,慕尼黑)和Smart A (WOODPECKER,桂林,中国)在使用树脂块的牵引/压缩台上进行连续旋转和往复运动的临床前比较。分别使用One Curve和One RECI器械(MICRO-MEGA, besanon, France)进行连续旋转和往复运动的根管整形。分析了根管成形过程中器械的穿透/移除力,使器械的切割效果和旋紧效果客观化。结果:结果表明:(1)牙髓马达影响牙髓器械的力学行为;(2)牙髓马达的影响主要体现在往复运动中;(3)往复角度影响牙髓器械的力学行为。结论:文献中广泛研究的只有根管器械,而根管马达对根管治疗有直接影响。本研究分析了根管马达对根管成形器械的影响。本研究倾向于证明Jeni马达可以优化根管器械的力学行为。
{"title":"Influence of endodontic motors on the behaviour of root canal shaping instruments: an in vitro comparative study.","authors":"Clémentine Reynette, Renaud Giess, Jeanne Davril, Jean-Marc Martrette, Éric Mortier, Rémy Balthazard, Marin Vincent","doi":"10.1038/s41405-023-00179-z","DOIUrl":"https://doi.org/10.1038/s41405-023-00179-z","url":null,"abstract":"<p><strong>Aims: </strong>The endodontic literature reports a lot of comparative study on endodontic instruments, concerning as well their geometry, instrumental dynamics, material, mechanical behavior or heat treatment. However, to our knowledge, no study has focused on the influence of endodontic motors on the shaping abilities of endodontic instruments. Thus, the aim of this study was to analyze the influence of the endodontic motors on root canal shaping instruments.</p><p><strong>Method: </strong>Dual Move (MICRO-MEGA, Besançon, France), Canal Pro CL2i (COLTENE, Alstätten, Suisse), Canal Pro Jeni Motor (COLTENE, Alstätten, Suisse), Ai Motor (WOODPECKER, Guilin, China), Wave One motor (VDW, Postfach, Munich) and Smart A (WOODPECKER, Guilin, China) were pre-clinically compared in continuous rotation and reciprocating motion on a traction/compression bench using resin blocks. Canal shaping in continuous rotation and reciprocating motion were performed with One Curve and One RECI instruments (MICRO-MEGA, Besançon, France), respectively. The penetration/removal forces, making it possible to objectify the cutting effect and screwing effect of the instruments during root canal shaping, were analyzed.</p><p><strong>Results: </strong>The results showed (i) that endodontic motors influence the mechanical behavior of endodontic instruments, (ii) that the influence of the motors is essentially felt during reciprocating motion and (iii) that the reciprocating angles influence the mechanical behavior of endodontic instruments.</p><p><strong>Conclusion: </strong>Only endodontic instruments are widely studied in literature while endodontic motors have a direct influence on root canal treatment. This study analyzes the influence of the endodontic motors on root canal shaping instruments. This study tends to demonstrate that Jeni Motor could optimize the mechanical behavior of endodontic instruments.</p>","PeriodicalId":36997,"journal":{"name":"BDJ Open","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2023-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10687023/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138463229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
3D-printed TCP-HA scaffolds delivering MicroRNA-302a-3p improve bone regeneration in a mouse calvarial model. 传递MicroRNA-302a-3p的3d打印TCP-HA支架改善小鼠颅骨模型的骨再生。
IF 3 Q2 Dentistry Pub Date : 2023-11-24 DOI: 10.1038/s41405-023-00177-1
Pirawish Limlawan, Numpon Insin, Laurine Marger, Mélanie Freudenreich, Stéphane Durual, Anjalee Vacharaksa

Objective: To demonstrate hydroxyapatite nanoparticles modified with cationic functional molecules. 3-aminopropyltriethoxysilane (HA-NPs-APTES) carrying microRNA-302a-3p (miR) in the 3D-printed tricalcium phosphate/Hydroxyapatite (TCP/HA) scaffold can increase healing of the critical-sized bone defect.

Materials and methods: 3D-printed TCP/HA were modified with HA-NPs-APTES by two methods (M1, M2). The dispersion of particles was visualized by fluorescent microscopy. Biocompatibility of the scaffolds was tested by alizarin assay. Delivery of miR to the cells and osteogenic gene expression were evaluated by qPCR. After selecting best method (M2), scaffolds, scaffolds+HA-NPs-APTES with or without miR were implanted in 4 mm mouse calvarium defect (n = 4 per group). After 2,4 and 6 weeks, bone regeneration were evaluated by microCT and histology sections.

Results: Both M1 and M2 scaffolds were biocompatible with cell adhesion on its surface. M2 scaffold showed significant increase of miR, suggesting successful delivery, resulted in downregulation of its target mRNA COUP-TFII, and upregulation of RUNX2 mRNA. Calvarium defect with M2 scaffold also showed significantly higher BV/TV and higher number of filled spaces at all time points. Histomorphometry demonstrated new bone formed at the center of the HA-NPs-APTES-miR scaffold earlier than controls.

Conclusion: TCP/HA scaffold modified with HA-NPs-APTES facilitated delivery of miR and enhanced bone regeneration.

目的:制备阳离子功能分子修饰的羟基磷灰石纳米粒。在3d打印的磷酸三钙/羟基磷灰石(TCP/HA)支架中携带microRNA-302a-3p (miR)的3-氨基丙基三乙氧基硅烷(HA- nps - aptes)可以促进临界尺寸骨缺损的愈合。材料与方法:采用HA- nps - aptes两种方法(M1, M2)对3d打印的TCP/HA进行修饰。用荧光显微镜观察颗粒的分散情况。采用茜素法检测支架的生物相容性。通过qPCR检测miR在细胞中的表达和成骨基因的表达。选择最佳方法(M2)后,将支架、支架+HA-NPs-APTES(含或不含miR)植入4 mm小鼠颅骨缺损(每组n = 4)。2周、4周和6周后,通过显微ct和组织学切片评估骨再生情况。结果:M1和M2支架表面细胞粘附,具有良好的生物相容性。M2支架miR明显升高,提示递送成功,导致其靶mRNA COUP-TFII下调,RUNX2 mRNA上调。在所有时间点,M2支架的颅骨缺损也显示出更高的BV/TV和更多的填充空间。组织形态学测定显示,HA-NPs-APTES-miR支架中心的新骨形成时间比对照组早。结论:HA- nps - aptes修饰的TCP/HA支架促进miR的传递,增强骨再生。
{"title":"3D-printed TCP-HA scaffolds delivering MicroRNA-302a-3p improve bone regeneration in a mouse calvarial model.","authors":"Pirawish Limlawan, Numpon Insin, Laurine Marger, Mélanie Freudenreich, Stéphane Durual, Anjalee Vacharaksa","doi":"10.1038/s41405-023-00177-1","DOIUrl":"10.1038/s41405-023-00177-1","url":null,"abstract":"<p><strong>Objective: </strong>To demonstrate hydroxyapatite nanoparticles modified with cationic functional molecules. 3-aminopropyltriethoxysilane (HA-NPs-APTES) carrying microRNA-302a-3p (miR) in the 3D-printed tricalcium phosphate/Hydroxyapatite (TCP/HA) scaffold can increase healing of the critical-sized bone defect.</p><p><strong>Materials and methods: </strong>3D-printed TCP/HA were modified with HA-NPs-APTES by two methods (M1, M2). The dispersion of particles was visualized by fluorescent microscopy. Biocompatibility of the scaffolds was tested by alizarin assay. Delivery of miR to the cells and osteogenic gene expression were evaluated by qPCR. After selecting best method (M2), scaffolds, scaffolds+HA-NPs-APTES with or without miR were implanted in 4 mm mouse calvarium defect (n = 4 per group). After 2,4 and 6 weeks, bone regeneration were evaluated by microCT and histology sections.</p><p><strong>Results: </strong>Both M1 and M2 scaffolds were biocompatible with cell adhesion on its surface. M2 scaffold showed significant increase of miR, suggesting successful delivery, resulted in downregulation of its target mRNA COUP-TFII, and upregulation of RUNX2 mRNA. Calvarium defect with M2 scaffold also showed significantly higher BV/TV and higher number of filled spaces at all time points. Histomorphometry demonstrated new bone formed at the center of the HA-NPs-APTES-miR scaffold earlier than controls.</p><p><strong>Conclusion: </strong>TCP/HA scaffold modified with HA-NPs-APTES facilitated delivery of miR and enhanced bone regeneration.</p>","PeriodicalId":36997,"journal":{"name":"BDJ Open","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2023-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10673873/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138435211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Soft tissue expansion using self-inflating osmotic hydrogel expanders prior to bone augmentation: healing and complications. Evidence-based review. 骨增强前使用自充气渗透性水凝胶扩张器进行软组织扩张:愈合和并发症。基于证据的审查。
IF 3 Q2 Dentistry Pub Date : 2023-11-11 DOI: 10.1038/s41405-023-00175-3
Adam Gade Ellesøe, Rawand Shado, Ines Novo Pereira, David Madruga, Haidar Hassan

Aim: This review aims to assess complication rates, soft tissue gain, and bone gain associated with the use of self-inflating osmotic hydrogel tissue expanders (SOHTEs) for soft tissue expansion (STE).

Methods: A comprehensive search on Pubmed and Google Scholar databases was conducted to identify human studies using SOHTEs for STE; last searched in March 2023. Expansion phase details and expander variables were documented. Complication rates, soft tissue gain, and bone gain reported in each study were also recorded. The inclusion criteria encompassed human studies ranging from evidence levels II-IV (Oxford Centre for Evidence-Based Medicine Levels of Evidence), without specific date limits. For assessing bias in randomized controlled trials (RCTs), a Risk of Bias tool was employed. The synthesised results were presented through tables, sunburst plots, and bar charts.

Results: A total of 13 studies were identified, comprising 4 RCTs, 1 cohort study, and 8 case-series. Employment of SOHTEs yielded an overall complication rate of 17% (24/140 sites), with expander perforation accounting for 9.3% (13/140) of the sites. Specific complication rates included dehiscence (1.4%, 2/140 sites), paraesthesia (1.4%, 2/140 sites), and infection (1.4%, 2/140 sites). All randomized controlled trials (RCTs) were categorised at Level II. The remaining investigations primarily consisted of Level IV case-series lacking controls. All studies demonstrated some concerns towards bias.

Conclusion: STE studies using SOHTEs exhibit a reduction in complications associated with bone augmentation in scenarios of inadequate soft tissue coverage. Preliminary evidence suggests potential benefits even in cases with sufficient soft tissue. Adherence to procedural precautions may reduce the risk of expander perforations, further diminishing complications. Subsequent studies should incorporate individual patient and expander variables in their reports to explore the impact of expansion phases on complication rates, as well as bone and soft tissue augmentation.

目的:本综述旨在评估与使用自充气渗透性水凝胶组织扩张器(SOHTEs)进行软组织扩张(STE)相关的并发症发生率、软组织增加和骨增加。方法:对Pubmed和谷歌Scholar数据库进行全面检索,以确定使用SOHTEs进行STE的人类研究;最后一次搜索是在2023年3月。扩展阶段的细节和扩展器变量被记录下来。同时记录每项研究报告的并发症发生率、软组织增重和骨增重。纳入标准包括从证据等级II-IV(牛津循证医学中心证据等级)的人类研究,没有具体的日期限制。为了评估随机对照试验(rct)的偏倚,使用了偏倚风险工具。综合结果通过表格、日蚀图和柱状图呈现。结果:共纳入13项研究,包括4项随机对照试验、1项队列研究和8项病例系列。使用sohte的总并发症发生率为17%(24/140个部位),其中扩张器穿孔占9.3%(13/140)。具体并发症发生率包括裂开(1.4%,2/140个位点)、感觉异常(1.4%,2/140个位点)和感染(1.4%,2/140个位点)。所有随机对照试验(rct)均被分类为II级。其余的调查主要包括缺乏控制的四级病例系列。所有的研究都表明了对偏见的一些担忧。结论:在软组织覆盖不足的情况下,使用SOHTEs的STE研究显示与骨增强相关的并发症减少。初步证据表明,即使在软组织充足的情况下也有潜在的益处。遵守手术预防措施可以降低扩张器穿孔的风险,进一步减少并发症。后续研究应在报告中纳入个体患者和扩张器变量,以探讨扩张阶段对并发症发生率以及骨和软组织扩张的影响。
{"title":"Soft tissue expansion using self-inflating osmotic hydrogel expanders prior to bone augmentation: healing and complications. Evidence-based review.","authors":"Adam Gade Ellesøe, Rawand Shado, Ines Novo Pereira, David Madruga, Haidar Hassan","doi":"10.1038/s41405-023-00175-3","DOIUrl":"10.1038/s41405-023-00175-3","url":null,"abstract":"<p><strong>Aim: </strong>This review aims to assess complication rates, soft tissue gain, and bone gain associated with the use of self-inflating osmotic hydrogel tissue expanders (SOHTEs) for soft tissue expansion (STE).</p><p><strong>Methods: </strong>A comprehensive search on Pubmed and Google Scholar databases was conducted to identify human studies using SOHTEs for STE; last searched in March 2023. Expansion phase details and expander variables were documented. Complication rates, soft tissue gain, and bone gain reported in each study were also recorded. The inclusion criteria encompassed human studies ranging from evidence levels II-IV (Oxford Centre for Evidence-Based Medicine Levels of Evidence), without specific date limits. For assessing bias in randomized controlled trials (RCTs), a Risk of Bias tool was employed. The synthesised results were presented through tables, sunburst plots, and bar charts.</p><p><strong>Results: </strong>A total of 13 studies were identified, comprising 4 RCTs, 1 cohort study, and 8 case-series. Employment of SOHTEs yielded an overall complication rate of 17% (24/140 sites), with expander perforation accounting for 9.3% (13/140) of the sites. Specific complication rates included dehiscence (1.4%, 2/140 sites), paraesthesia (1.4%, 2/140 sites), and infection (1.4%, 2/140 sites). All randomized controlled trials (RCTs) were categorised at Level II. The remaining investigations primarily consisted of Level IV case-series lacking controls. All studies demonstrated some concerns towards bias.</p><p><strong>Conclusion: </strong>STE studies using SOHTEs exhibit a reduction in complications associated with bone augmentation in scenarios of inadequate soft tissue coverage. Preliminary evidence suggests potential benefits even in cases with sufficient soft tissue. Adherence to procedural precautions may reduce the risk of expander perforations, further diminishing complications. Subsequent studies should incorporate individual patient and expander variables in their reports to explore the impact of expansion phases on complication rates, as well as bone and soft tissue augmentation.</p>","PeriodicalId":36997,"journal":{"name":"BDJ Open","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2023-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10640575/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89719879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
BDJ Open
全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1