Ecological Genetics and Genomics最新文献_第5页

The growth and biochemical traits including nutrient contents of fourteen commercial almond (Prunus sp.) genotypes affected by drought stress 干旱胁迫对14个商品杏仁（Prunus sp.）基因型生长及营养成分生化性状的影响

Q3 Agricultural and Biological Sciences

Ecological Genetics and Genomics

Pub Date : 2025-10-01 DOI: 10.1016/j.egg.2025.100410

Esmaeil Safavi Bakhtiari , Mehrab Yadegari , Asghar Mousavi , Bijan Haghighati

Due to its health and economic significance, almond (Prunus sp.) is largely grown in the arid and semi-arid areas. Drought stress affecting morphological and physiological characteristics of 14 young almond genotypes, during middle- and late--growth stages, was investigated in the field. The experiments (2020 and 2021) were split plot on the basis of randomized complete block design (RCBD) with three replicates investigating irrigation at 70 and 30 % of field moisture capacity (main factor) and 14 genotypes of almond vegetative rootstock (sub-factor) grafted on GN rootstock. Almond genotypes responses were different to drought stress. Although control treatment resulted in the highest height, stem diameter and leaf area of genotypes, drought stress significantly reduced growth of almond trees and increased leaf abscission. Considerable variations were observed among almond genotypes in terms of H₂O₂, and nutrient contents, during growing season in both years. Stressed Mamaei genotype with the least growth rate had the highest H₂O₂ content (19.99 μmol g⁻¹ FW). Plant nutrient uptake (excluding K and Fe) decreased in stressed genotypes. N content of GN genotype was the highest by control (3.54 %) and stress (2.52 %) treatments. Growth stage stress significantly affected plant growth and nutrient uptake. The highest Fe content was obtained in stressed GN genotype, and stressed Shahrood 13 and Shahrood 7 genotypes had the lowest Fe contents in both growth stages. Planting the most tolerant almond genotypes along with K fertilization may be one of the most effective methods to enhance almond growth and physiology in drought stressed conditions.

由于其健康和经济意义，杏仁（Prunus sp.）主要生长在干旱和半干旱地区。在田间研究了干旱胁迫对14个幼杏仁基因型生长中后期形态生理特性的影响。试验（2020年和2021年）采用随机完全区组设计（RCBD），采用3个重复，分别以70%和30%的田间水分灌溉（主因子）和14个基因型的杏仁营养砧木（子因子）嫁接GN砧木。杏仁基因型对干旱胁迫的响应不同。虽然对照处理的杏树株高、茎粗和叶面积最高，但干旱胁迫显著降低了杏树的生长，增加了叶片脱落。在两个年份的生长季节，杏仁基因型在H2O2和养分含量方面存在较大差异。胁迫马氏菌基因型的H2O2含量最高，为19.99 μmol g−1 FW，生长速率最低。胁迫基因型的植株养分吸收（不包括钾和铁）降低。GN基因型氮素含量以对照（3.54%）和胁迫（2.52%）处理最高。生育期胁迫对植物生长和养分吸收有显著影响。在两个生育期，胁迫GN基因型铁含量最高，胁迫Shahrood 13和Shahrood 7基因型铁含量最低。在干旱胁迫条件下，种植最耐受性最强的杏仁基因型并配以钾肥可能是促进杏仁生长和生理机能的最有效方法之一。

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引用次数: 0

Analysis of microbiomes in irrigation water, soil and lettuce from community farms in the Phoenix metro area 凤凰城城区社区农场灌溉用水、土壤和生菜微生物组分析

Q3 Agricultural and Biological Sciences

Ecological Genetics and Genomics

Pub Date : 2025-10-01 DOI: 10.1016/j.egg.2025.100409

Saul Lopez-Salas , Theresa Morales , Theodore M. Ransome , Marianne Smith , Sudipta Biswas

This research compares the bacterial profile in soil, irrigation water, and lettuce from farms located in food deserts (FD; low income and limited access to grocery stores), and non-food deserts (n-FD; high income and easier food access) in the Phoenix metropolitan area. It was hypothesized that farms from FD areas would have high coliform bacteria in soil, irrigation water, and lettuce compared to n-FD farms and appropriate controls. Serially diluted soil samples were cultured on agar plates for bacterial growth. Meanwhile, water samples were tested for coliform bacteria, nitrate, phosphate, temperature, pH levels, and were plated on petri film to identify any coliform presence. DNA extracted from lettuce, sequenced on the Illumina Nextseq2000 Sequencing System was used to create the 16S rRNA sequencing data. Soil results indicated that FD had more total coliform bacteria and high E. coli compared to n-FD and controls. Only some of the water samples from FD showed a statistically significant presence of coliform bacteria in irrigation water. The lettuce microbial profile revealed that FD had a 90% abundance of cyanobacteria and 4–8% abundance in proteobacteria like Pseudomonas and Enterobacteriales like E. coli. n-FD farms showed a predominance of facultative anaerobes from family Marinilabiliaceae and 2% abundance of Proteobacteria like Pseudomonas, Acinetobacter, and Staphylococcus. Phylogenetic tree mapping, revealed the presence of certain pathogenic bacteria like Pseudomonas in all the samples. Further research is needed to identify the source of bacterial contamination in the soil and to develop strategies to control their spread in fresh produce.

这项研究比较了凤凰城大都市区位于食物沙漠（FD；低收入和进入杂货店的机会有限）和非食物沙漠（n-FD；高收入和更容易获得食物）的农场的土壤、灌溉水和生菜中的细菌特征。据推测，与非FD农场和适当的对照相比，FD地区的农场在土壤、灌溉水和生菜中有较高的大肠菌群。连续稀释的土壤样品在琼脂板上培养细菌生长。同时，对水样进行了大肠菌群细菌、硝酸盐、磷酸盐、温度、pH值的检测，并将水样镀在培养皿上以确定大肠菌群的存在。从生菜中提取DNA，在Illumina Nextseq2000测序系统上测序，用于创建16S rRNA测序数据。土壤结果表明，与n-FD和对照相比，FD有更多的总大肠菌群和高大肠杆菌。只有FD的部分水样显示灌溉水中大肠菌群的存在具有统计学意义。生菜微生物谱显示，FD的蓝藻菌丰度为90%，假单胞菌等变形菌和大肠杆菌等肠杆菌丰度为4-8%。n-FD养殖场显示，Marinilabiliaceae家族的兼性厌氧菌占主导地位，假单胞菌、不动杆菌和葡萄球菌等变形菌的丰度为2%。系统发育树图谱显示，所有样品中均存在假单胞菌等致病菌。需要进一步的研究来确定土壤中细菌污染的来源，并制定策略来控制它们在新鲜农产品中的传播。

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引用次数: 0

Engineering crop genomes: A review of editing tool evolution, applications, and future trajectories 工程作物基因组：编辑工具进化、应用和未来轨迹的综述

Q3 Agricultural and Biological Sciences

Ecological Genetics and Genomics

Pub Date : 2025-09-26 DOI: 10.1016/j.egg.2025.100408

Mandakini Kabi , Suvalaxmi Palei , Snehasish Routray , Islavath Suresh Naik , Mahipal Singh Kesawat , Vidadala Rajendra , Rajneesh Kumar , Mehdi Rahimi

Crop improvement requires precise modification of genotypes to enhance their usefulness for human need that encompasses a broad range of techniques. It started with conventional plant breeding techniques like domestication, selection, hybridization breeding, mutation breeding, and passes through molecular breeding. These techniques have pros and cons in terms of their procedure, application, and outcome. Therefore, efficient desirable manipulation of genotypes can achieve through genome editing techniques that includes inactivation of target gene, replacement or addition of new gene with new function to the genome. In the recent past, emergence of new approach of genome editing enables researcher to manipulate gene. It uses artificial nucleases to modify genome rapidly which allow reverse genetics, genome engineering and targeted transgene integration in to one experiment in an efficient, precise and predictable manner. Tools used for genome editing are, programmable sequence specific nucleases which includes Mega nucleases, ZFN, TALEN and CRISPR-cas9 and base editor. Use of this genome editing tools to get desirable characters has been reported in many crops. This review paper has elaborately focused on genome editing tools and desirable modification of characters for crop improvements.

作物改良需要对基因型进行精确的修改，以提高其对人类需求的有用性，这包括广泛的技术范围。它从传统的植物育种技术开始，如驯化、选择、杂交育种、突变育种，然后通过分子育种。这些技术在程序、应用和结果方面各有利弊。因此，通过基因组编辑技术，包括靶基因的失活、在基因组中替换或添加具有新功能的新基因，可以实现对基因型的有效操纵。近年来，基因组编辑新方法的出现使研究人员能够操纵基因。它利用人工核酸酶快速修饰基因组，使反向遗传学、基因组工程和靶向转基因整合以高效、精确和可预测的方式在一次实验中实现。用于基因组编辑的工具是可编程序列特异性核酸酶，包括Mega核酸酶，ZFN， TALEN和CRISPR-cas9和碱基编辑器。利用这种基因组编辑工具获得理想的性状已在许多作物中得到报道。这篇综述文章详细介绍了基因组编辑工具和作物改良所需的性状修改。

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引用次数: 0

Evaluation of yield traits and essential oil yield of Istanbul oregano (Origanum vulgare subsp. hirtum) genotypes across two Mediterranean environments 伊斯坦布尔牛至产量性状及挥发油产量评价。hirtum)基因型跨越两个地中海环境

Q3 Agricultural and Biological Sciences

Ecological Genetics and Genomics

Pub Date : 2025-09-13 DOI: 10.1016/j.egg.2025.100407

Olcay Arabacı , Emine Bayram , Çiğdem Sönmez , Gülsüm Boztaş , Nazlıcan Ataman , Elif Büyükkayhan

Origanum vulgare subsp. hirtum (Istanbul oregano) is a commercially valuable aromatic and medicinal plant widely used in the food, pharmaceutical, and cosmetic industries due to its high essential oil content and bioactive properties. This study evaluated the agronomic performance of 22 Istanbul oregano genotypes, including commercial varieties and selected clones, across two Mediterranean environments (Aydın and İzmir) over two years (2021–2022). Significant genotype × environment interactions were detected for plant height, fresh herb yield (FHY), drug herb yield (DHY), drug leaf yield (DLY), and essential oil yield (EOY) (p < 0.01). Average FHY ranged from 2.39 t ha⁻¹ in 2021 to 21.97 and 8.32 t ha⁻¹ in the first and second cuts of 2022, respectively. DHY increased from 0.75 to 1.01 t ha⁻¹ in 2021 to 6.50–5.81 t ha⁻¹ in 2022, while DLY peaked at 15.87 t ha⁻¹ during the first cut of 2022. Inter-annual climatic variation strongly influenced EOY. In the first cut of 2022, means reached 503.5 L ha⁻¹ in Aydın and 259.7 L ha⁻¹ in İzmir, 15- to 8-fold higher than the overall mean of 2021 (32.3 L ha⁻¹). However, summer drought and heat stress sharply reduced second cut yields, particularly in Aydın, where EOY declined by about 75 %. Genotypes such as C-417 and A-420 exhibited high plasticity and stability, while the commercial variety Tınmaz performed well only under favorable conditions. Genotypes combining stability and high yield across environments represent promising candidates for breeding and sustainable oregano production under Mediterranean climates.

土一枝亚种。伊斯坦布尔牛至是一种具有商业价值的芳香和药用植物，由于其高精油含量和生物活性特性，被广泛用于食品、制药和化妆品行业。本研究评估了22种伊斯坦布尔牛至基因型（包括商业品种和精选无性系）在两种地中海环境（Aydın和İzmir）中两年（2021-2022年）的农艺表现。株高、鲜草产量（FHY）、中药材产量（DHY）、药叶产量（DLY）和精油产量（EOY）的基因型与环境的交互作用显著（p < 0.01）。平均FHY范围从2021年的2.39 t / hm2到2022年第一次和第二次削减的21.97和8.32 t / hm2。DHY从2021年的0.75 - 1.01 t / ha - 1增加到2022年的6.50-5.81 t / ha - 1，而在2022年的第一次削减期间，DLY达到了15.87 t / ha - 1的峰值。年际气候变化强烈影响EOY。在2022年的第一次削减中，Aydın的平均值达到503.5 L ha - 1， İzmir的平均值达到259.7 L ha - 1，比2021年的总体平均值（32.3 L ha - 1）高出15至8倍。然而，夏季干旱和高温胁迫大大降低了二次减产产量，特别是Aydın， EOY下降了约75%。C-417和A-420等基因型表现出较高的可塑性和稳定性，而商品品种Tınmaz只有在有利条件下才能表现良好。在地中海气候条件下，结合稳定性和高产的基因型是培育和可持续牛至生产的有希望的候选者。

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引用次数: 0

Genome-wide identification and characterization of Dof gene family in sesame 芝麻Dof基因家族的全基因组鉴定与特征分析

Q3 Agricultural and Biological Sciences

Ecological Genetics and Genomics

Pub Date : 2025-09-08 DOI: 10.1016/j.egg.2025.100404

Md Nahid Hasan , Khandker Shazia Afrin , A.T.M. Majharul Mannan , Tasmina Islam Simi , Md Abdur Rahim

The plant-specific TF family ‘Dof’ comprises a highly conserved ‘Dof domain’, which is the DNA binding domain with one ‘Zn²⁺’ finger. It plays a crucial role in plant responses to environmental stimuli affecting growth and development. The Dof genes have been analyzed in many plants, including Arabidopsis, but have not yet been well studied in sesame on a genome-wide scale. In the present study, we reported for the first time 32 Dof genes in sesame, which are located across 12 linkage groups (LGs). The SiDof genes comprised one to five exons and zero to four introns, which is comparable to Dof genes in other species. Of 32 SiDof genes, about 71.88 % were intronless. The SiDof genes were classified into six groups and exhibited evolutionary relationships with other species. Group III contained the highest number of SiDof genes (seven), while the lowest number was in group V (three). There was a robust protein-protein interaction between NAC071 and two Dof proteins, namely DOF5.3 and DOF5.6. Total 1668 ‘cis-regulatory elements’ were identified in the promoter of 32 SiDof genes. Among these, 733 were linked to the stress responses, 562 to the light responses, and 373 to the phytohormone response. The accessible transcriptome (‘RNA-seq’) data-based expression profiles have shown that SiDof genes might be associated with various abiotic stresses, including drought (SiDof1) and salinity (SiDof32) in sesame. Nonetheless, further investigation on the functionality analysis of candidate SiDof genes is required.

植物特异性TF家族‘ Dof ’包含一个高度保守的‘ Dof结构域’，这是一个‘ Zn2+ ’指的DNA结合结构域。它在植物对影响生长发育的环境刺激的响应中起着至关重要的作用。Dof基因已经在包括拟南芥在内的许多植物中进行了分析，但尚未在全基因组范围内对芝麻进行了很好的研究。在本研究中，我们首次报道了芝麻中32个Dof基因，这些基因分布在12个连锁群（LGs）中。SiDof基因由1 ~ 5个外显子和0 ~ 4个内含子组成，这与其他物种的Dof基因相似。32个SiDof基因中，约71.88%为无内含子基因。SiDof基因被划分为6类，并显示出与其他物种的进化关系。ⅲ组SiDof基因数量最多（7个），V组最少（3个）。NAC071与DOF5.3和DOF5.6两种Dof蛋白之间存在较强的蛋白相互作用。在32个SiDof基因的启动子中共鉴定出1668个顺式调控元件。其中，733个与应激反应有关，562个与光照反应有关，373个与植物激素反应有关。基于转录组（' RNA-seq '）数据的表达谱显示，SiDof基因可能与芝麻中的干旱（SiDof1）和盐度（SiDof32）等多种非生物胁迫有关。尽管如此，候选SiDof基因的功能分析仍需进一步研究。

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引用次数: 0

Morphogenesis modulation of Rhizopus oryzae by peptone enhances lipase production during submerged fermentation 蛋白胨对米根霉形态发生的调节促进了深层发酵过程中脂肪酶的产生

Q3 Agricultural and Biological Sciences

Ecological Genetics and Genomics

Pub Date : 2025-09-05 DOI: 10.1016/j.egg.2025.100406

Ayda Torchi

Lipases are industrially important enzymes with broad applications in food, pharmaceuticals, and biofuels. However, their large-scale production remains challenging. This study aimed to enhance lipase productivity by modulating Rhizopus oryzae morphogenesis through the supplementation of different peptone sources under submerged fermentation. Distinct cellular macromorphologies was found to strongly influence growth performance and lipase productivity. Lipase production was enhanced six –fold compared to previous studies, with a maximum of 492 IU/mL recorded after 76h. Subsequent molecular analysis underscored the crucial role of key regulatory proteins in understanding the production systems of R.oryzae and how its metabolism adapts to various nitrogen sources. These findings offer a promising approach to employ innovative morphological engineering techniques to precisely adjust the morphological development of industrially relevant fungi.

脂肪酶是工业上重要的酶，在食品、制药和生物燃料中有着广泛的应用。然而，它们的大规模生产仍然具有挑战性。本研究旨在通过在深层发酵条件下添加不同蛋白胨源，调节米根霉的形态发生，从而提高其脂肪酶产量。发现不同的细胞大形态强烈影响生长性能和脂肪酶产量。与之前的研究相比，脂肪酶的产量提高了6倍，76h后最高达到492 IU/mL。随后的分子分析强调了关键调节蛋白在理解r.m oryzae生产系统及其代谢如何适应各种氮源方面的关键作用。这些发现为采用创新的形态工程技术来精确调整工业相关真菌的形态发展提供了一种有希望的方法。

引用次数: 0

Growth and antioxidant enzymes responses of Aegilops tauschii ecotypes to drought stress: Identifying tolerant genotypes 黄颡鱼生态型对干旱胁迫的生长和抗氧化酶响应：耐受性基因型的鉴定

Q3 Agricultural and Biological Sciences

Ecological Genetics and Genomics

Pub Date : 2025-09-04 DOI: 10.1016/j.egg.2025.100405

Elnaz Nozari , Rasool Asghari-Zakaria , Nasser Zare , Parisa Sheikhzadeh

Drought is a significant environmental challenge that restricts crop growth and development. Aegilops tauschii, the source of the DD genome (2n = 2x = 14), is an important genetic resource for enhancing drought tolerance in wheat. This study investigated 15 native ecotypes of Ae. tauschii under three levels of water availability: non-stress (100 % field capacity), moderate stress (60 % FC), and severe stress (30 % FC). We evaluated various traits, including shoot dry weight (SDW), tiller number (TN), plant length (PL), dry root weight (DRW), and root volume (RV), along with activities of peroxidase (POD) and polyphenol oxidase (PPO) to assess drought tolerance. Significant variability in drought responses was observed; growth metrics declined under water stress, especially during severe conditions. POD and PPO activities increased notably during drought, indicating differences between control and stressed plants. Based on Membership Function Value (MFV), the ecotype E6 showed the highest drought tolerance (MFV >0.83), and E8 placed in second order (MFV >0.70), while E1, E3, and E12 were the most drought-sensitive (MFV <0.30). Other ecotypes exhibited moderate to varying levels of tolerance. Based on the heatmap and cluster analysis, the 15 Ae. tauschii ecotypes were grouped into four main clusters according to their responses to drought stress. These results highlight E6 and E8 as promising candidates for improving drought tolerance in wheat, due to their stable growth, high antioxidant enzyme activity, and consistent ranking across both MFV and clustering analyses.

干旱是制约作物生长发育的重大环境挑战。作为DD基因组（2n = 2x = 14）的来源，陶氏Aegilops tauschii是提高小麦抗旱性的重要遗传资源。本研究调查了15个本地生态型伊蚊。tauschii在三个水可用性水平下：非胁迫（100%田间容量），中等胁迫（60% FC）和严重胁迫（30% FC）。通过对茎部干重（SDW）、分蘖数（TN）、株长（PL）、干根重（DRW）、根体积（RV）、过氧化物酶（POD）和多酚氧化酶（PPO）活性等性状的评价来评价植物的抗旱性。观察到干旱反应的显著变化；生长指标在水分胁迫下下降，特别是在恶劣条件下。POD和PPO活性在干旱期间显著升高，表明对照和胁迫植株之间存在差异。隶属函数值（Membership Function Value， MFV）显示，E6的耐旱性最高（MFV >0.83）， E8次之（MFV >0.70）， E1、E3和E12对干旱最敏感（MFV <0.30）。其他生态型表现出中等到不同程度的耐受性。基于热图和聚类分析，15个Ae。根据不同生态型对干旱胁迫的响应，将黄家菜生态型划分为4个主要类群。这些结果表明，由于E6和E8生长稳定，抗氧化酶活性高，并且在MFV和聚类分析中排名一致，因此它们是提高小麦耐旱性的有希望的候选者。

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引用次数: 0

Metagenomic profiling reveals microbial diversity and MicroTrait bioelement signatures in Ethiopian soda lakes 宏基因组分析揭示了埃塞俄比亚苏打湖微生物多样性和微特征生物元素特征

Q3 Agricultural and Biological Sciences

Ecological Genetics and Genomics

Pub Date : 2025-09-03 DOI: 10.1016/j.egg.2025.100403

Gessesse Kebede Bekele , Ebrahim M. Abda , Abu Feyisa Meka , Ermias Sissay Balcha , Zuriash Mamo Nademo , Fassil Assefa Tuji , Mesfin Tafesse Gemeda

Lake Chitu and Shala, two Ethiopian soda lakes, are polyextreme environments characterized by high salinity and alkalinity. These conditions support diverse haloalkaliphilic microbial communities that regulate critical biogeochemical cycles. However, their MicroTrait bioelement profiles remain poorly characterized. In this study, shotgun metagenomic sequencing was employed to investigate taxonomic composition and bioelement-associated functional genes derived from MicroTrait analysis in Lakes Chitu and Shala, providing insights into microbial contributions to biogeochemical cycling. Environmental samples were collected, and DNA was extracted and sequenced to generate comprehensive metagenomic datasets. Taxonomic annotation revealed substantial phylogenetic diversity in both lakes. Bacteria dominated the communities (89 % in Chitu, 92 % in Shala), with Archaea present in lower abundances (4 % in Chitu, 0.8 % in Shala), alongside minor fractions of eukaryotes and viruses. The presence of unclassified and unassigned clades suggests potentially novel microbial lineages. Dominant bacterial phyla included Pseudomonadota, Actinomycetota, and Gemmatimonadota, while Euryarchaeota and Nitrososphaerota were the main archaeal groups. MicroTrait-based comparative metagenomic profiling using Annotated-Metagenome-Assemblies (AMAs) revealed both shared and distinct elemental cycling potentials in microbial communities from Lake Chitu and Shala. While Chitu exhibited higher gene hits for nitrogen, carbon fixation, and methane metabolism, Shala showed greater functional capacity in oxygen respiration, sulfur oxidation, C₁ metabolism, and trace element detoxification, highlighting ecosystem-specific biogeochemical specializations. These findings reveal distinct MicroTrait bioelement gene abundance profiles, uncovering metabolic strategies and genetic potential in these extreme ecosystems. This underscores the importance of linking microbial diversity to functional traits and highlights potential biotechnological applications.

埃塞俄比亚的奇图湖和沙拉湖是高盐度、高碱度的多极端环境。这些条件支持调节关键生物地球化学循环的不同的嗜盐碱性微生物群落。然而，它们的微性状生物元素特征仍然很差。本研究采用霰弹枪宏基因组测序技术，对赤图湖和沙拉湖微生物的分类组成和生物元素相关功能基因进行了分析，为微生物在生物地球化学循环中的作用提供了新的思路。收集环境样本，提取DNA并测序，生成全面的宏基因组数据集。分类学注释显示两个湖泊具有丰富的系统发育多样性。细菌在群落中占主导地位（Chitu占89%，Shala占92%），古细菌的丰度较低（Chitu占4%，Shala占0.8%），真核生物和病毒也占少数。未分类和未分配分支的存在表明可能存在新的微生物谱系。优势菌门包括假单胞菌门、放线菌门和双胞菌门，而Euryarchaeota和亚硝基球藻门是主要的古细菌类群。基于微性状的宏基因组比较分析揭示了赤图湖和沙拉湖微生物群落中元素循环电位的共性和差异性。赤兔在氮、碳固定和甲烷代谢方面表现出更高的基因击中，而沙拉在氧呼吸、硫氧化、C1代谢和微量元素解毒方面表现出更大的功能能力，突出了生态系统特异性的生物地球化学特化。这些发现揭示了不同的MicroTrait生物元素基因丰度谱，揭示了这些极端生态系统的代谢策略和遗传潜力。这强调了将微生物多样性与功能性状联系起来的重要性，并突出了潜在的生物技术应用。

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引用次数: 0

Taxonomic identification of stingless bees (Hymenoptera: Apidae: Meliponini) from selected locations of Tanzania using DNA barcoding 利用DNA条形码技术鉴定坦桑尼亚部分地区无刺蜜蜂（膜翅目：蜂科：Meliponini）的分类

Q3 Agricultural and Biological Sciences

Ecological Genetics and Genomics

Pub Date : 2025-09-03 DOI: 10.1016/j.egg.2025.100402

Christopher Alphonce Mduda , Faraja Samwel Makwinja , Juma Mahmud Hussein

Accurate taxonomic identification of stingless bees is critical for their conservation and sustainable management, yet morphological methods are often limited by species complexity and lack of expertise. This study employed DNA barcoding targeting a 650 bp fragment of the mitochondrial cytochrome oxidase I (COI) gene to identify stingless bee specimens collected from 19 locations across mainland Tanzania. A total of 28 specimens from wild colonies were analyzed, yielding reliable species-level identification (>97 % similarity to reference sequences in the BOLD database) for 53.6 % of the samples. Identified species included Plebeina armata, Hypotrigona gribodoi, Axestotrigona ferruginea, and Dactylurina schmidti. Specimens with barcode similarity between 95 and 97 % were assigned to the genus Axestotrigona, with their taxonomic status remaining unresolved. Despite reliable identification, we observed significant morphological diversity among P. armata and H. gribodoi specimens, with average within-group genetic distances of 3.5 and 4.1 %, respectively. Phylogenetic analysis corroborated these identifications and revealed potential cryptic speciation and genetic structuring consistent with geographical locations. The findings underscore the utility of DNA barcoding to complement traditional taxonomic approaches, and highlight the underexplored diversity of Afrotropical stingless bees. Further molecular and morphological studies are recommended to clarify species boundaries within the genus Axestotrigona, resolve taxonomic discrepancies in Afrotropical stingless bees, and improve regional biodiversity assessments.

准确的无刺蜜蜂分类鉴定对其保护和可持续管理至关重要，但形态学方法往往受到物种复杂性和缺乏专业知识的限制。本研究采用DNA条形码技术，针对线粒体细胞色素氧化酶I （COI）基因的650 bp片段，鉴定了从坦桑尼亚大陆19个地点收集的无刺蜜蜂标本。共分析了来自野生菌落的28个标本，对53.6%的样本进行了可靠的物种水平鉴定（与BOLD数据库中的参考序列相似度为97%）。已鉴定的种类包括：斑胸草属（Plebeina armata）、拟三角草属（Hypotrigona gribodoi）、铁三角草属（Axestotrigona ferruginea）和施米草属（Dactylurina schmidti）。条形码相似性在95% ~ 97%之间的标本归属于Axestotrigona属，其分类地位尚未确定。尽管鉴定可靠，但我们观察到armata和H. gribodoi的形态多样性显著，群内遗传距离平均分别为3.5%和4.1%。系统发育分析证实了这些鉴定，并揭示了潜在的隐种形成和与地理位置一致的遗传结构。这一发现强调了DNA条形码在补充传统分类学方法方面的实用性，并强调了非洲热带无刺蜜蜂尚未被充分探索的多样性。建议进一步开展分子形态学研究，以明确无刺蜂属的物种界限，解决非洲热带无刺蜂的分类差异，提高区域生物多样性评价。

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引用次数: 0

Corrigendum to “Quantitative trait loci (QTLs) mapping of seed coat colors in sesame (Sesamum indicum L.): A review” [Ecologic. Genet. Genom. 36 (2025) 100384] 芝麻（Sesamum indicum L.）种皮颜色的数量性状位点（qtl）定位综述[生态学]。麝猫。基因组。36 (2025)100384]

Q3 Agricultural and Biological Sciences

Ecological Genetics and Genomics

Pub Date : 2025-09-01 DOI: 10.1016/j.egg.2025.100389

Diriba Shanko , Mebeaselassie Andargie

引用次数: 0