Pub Date : 2024-04-24DOI: 10.1016/j.egg.2024.100251
Laxmikant Sambhaji Kokate , Sanjeev Kumar , Ananta Kumar Das , Abdul Rahim , Jowel Debnath
Understanding the basal expression kinetics of genes related to innate immunity aids effective genetic selection for immune response and how host genetics behaves with tolerance to Newcastle disease (ND) is obscure. It was hence aimed to investigate post-immunization mRNA expression kinetics of some immune response genes in White Leghorn, Aseel and Kadaknath chicken. Day-old chicks were vaccinated with ND RDF-1 strain with booster dose at 28-days of age at the experimental layer farm of ICAR-Central Avian Research Institute, Izatnagar (India) and lymphoid tissues were collected in RNAlater at 42-days of age. Total RNA was extracted and first strand cDNA was synthesized. Relative quantification of mRNA expression of IL1-β, IFN-γ, iNOS and TLR15 gene was assessed by qRT-PCR. The mRNA expressions significantly varied among the genes in spleen and thymus, and moderate expression of IFN-γ in all chicken genotypes suggested good protection against Newcastle disease virus. IL1-β-mRNA expressed at the highest level (P˂ 0.05) in spleen followed by thymus and bursa, whereas IFN-γ and iNOS expressed highly (P˂ 0.05) in thymus followed by spleen and bursa. In case of TLR15, the expression was significantly the highest in spleen followed by bursa and thymus. Kadaknath had the highest (P˂ 0.05) expression of IL1-β in thymus and that of iNOS in spleen, whereas Aseel showed the least (P > 0.05) expression than Kadaknath and White Leghorn. The study revealed the existence of wide variation in basal expression levels of immune response genes among different lymphoid tissues and lack of discernible differences in the expression between the chicken genotypes.
{"title":"Investigation of post-immunization immune response gene expression kinetics in lymphoid tissues of White Leghorn and Indian native chicken","authors":"Laxmikant Sambhaji Kokate , Sanjeev Kumar , Ananta Kumar Das , Abdul Rahim , Jowel Debnath","doi":"10.1016/j.egg.2024.100251","DOIUrl":"10.1016/j.egg.2024.100251","url":null,"abstract":"<div><p>Understanding the basal expression kinetics of genes related to innate immunity aids effective genetic selection for immune response and how host genetics behaves with tolerance to Newcastle disease (ND) is obscure. It was hence aimed to investigate post-immunization mRNA expression kinetics of some immune response genes in White Leghorn, Aseel and Kadaknath chicken. Day-old chicks were vaccinated with ND RDF-1 strain with booster dose at 28-days of age at the experimental layer farm of ICAR-Central Avian Research Institute, Izatnagar (India) and lymphoid tissues were collected in RNAlater at 42-days of age. Total RNA was extracted and first strand cDNA was synthesized. Relative quantification of mRNA expression of IL1-β, IFN-γ, iNOS and TLR15 gene was assessed by qRT-PCR. The mRNA expressions significantly varied among the genes in spleen and thymus, and moderate expression of IFN-γ in all chicken genotypes suggested good protection against Newcastle disease virus. IL1-β-mRNA expressed at the highest level (P˂ 0.05) in spleen followed by thymus and bursa, whereas IFN-γ and iNOS expressed highly (P˂ 0.05) in thymus followed by spleen and bursa. In case of TLR15, the expression was significantly the highest in spleen followed by bursa and thymus. Kadaknath had the highest (P˂ 0.05) expression of IL1-β in thymus and that of iNOS in spleen, whereas Aseel showed the least (P > 0.05) expression than Kadaknath and White Leghorn. The study revealed the existence of wide variation in basal expression levels of immune response genes among different lymphoid tissues and lack of discernible differences in the expression between the chicken genotypes.</p></div>","PeriodicalId":37938,"journal":{"name":"Ecological Genetics and Genomics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140782120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-24DOI: 10.1016/j.egg.2024.100254
Esther Anokye, Ebenezer Obeng-Bio, Abraham Akpertey, Moses Kwame Aidoo, Margaret Saka Aduama-Larbi, Francis Kwame Padi
Within the cocoa germplasm used for variety development in West Africa, knowledge of varieties that are suitable as rootstocks to promote growth and precocity of clones is lacking. Six cocoa varieties were used to generate 36 clones for evaluation. For each of the six scions selected as ortets from previous progeny trials, the corresponding seed-derived family was regenerated through manual pollinations. The scions were grafted in a full diallel fashion on each of the six seed-derived family, and field evaluated in four replications following a randomized complete block design. Also, seed-derived varieties were evaluated in the nursery for vigour characteristics, and under similar conditions as the 36 clones in the field. Genotype heterozygosity at 72 SNP loci was significantly correlated with vigour characteristics of the six varieties. Seed-derived varieties of high vigour benefited clone performance for vigour, and dry bean yield when used as rootstocks but not as scion. The maximum photochemical efficiency of photosystem II (Fv/Fm) was different among seed-derived varieties and clones in the dry season but not the rainy season. Among clones, higher Fv/Fm values were associated with higher-vigour rootstock varieties. Diallel grafting model enabled partitioning of total variance into several sub-components. The variance due to specific compatibility ability was not significant for any of the measured traits. Decomposition of the reciprocal variance revealed that significant reciprocal effects were mainly due to significant maternal (rootstock) effects. The key finding is that, for any scion, the most precocious clone is obtained by grafting on high vigour rootstock varieties.
在西非用于品种开发的可可种质中,缺乏适合作为砧木以促进克隆体生长和早熟的品种的知识。我们利用六个可可品种培育出 36 个克隆进行评估。对于从以前的后代试验中选出作为砧木的六个接穗,都通过人工授粉再生出了相应的种源家族。接穗以全对生方式嫁接在六个种子衍生系列上,并按照随机完全区组设计在四个重复中进行田间评估。此外,还在苗圃中对种子衍生品种的活力特征进行了评估,并在与 36 个克隆品种相似的条件下进行了田间评估。72 个 SNP 位点的基因型杂合度与六个品种的活力特征有显著相关性。当用作砧木而非接穗时,种子衍生的高活力品种有利于克隆的活力表现和干豆产量。种子衍生品种和克隆品种的光系统 II 最大光化学效率(Fv/Fm)在旱季不同,而在雨季不同。在克隆品种中,Fv/Fm 值越高的砧木品种活力越强。通过 Diallel 嫁接模型,可将总变异分为几个子成分。对于任何测得的性状而言,由特定相容性引起的变异都不显著。互变分解显示,显著的互变效应主要是由于显著的母本(砧木)效应造成的。主要发现是,对于任何接穗,通过嫁接在高活性砧木品种上都能获得最早熟的克隆。
{"title":"Rootstock influences on growth and bearing precocity in juvenile cocoa","authors":"Esther Anokye, Ebenezer Obeng-Bio, Abraham Akpertey, Moses Kwame Aidoo, Margaret Saka Aduama-Larbi, Francis Kwame Padi","doi":"10.1016/j.egg.2024.100254","DOIUrl":"10.1016/j.egg.2024.100254","url":null,"abstract":"<div><p>Within the cocoa germplasm used for variety development in West Africa, knowledge of varieties that are suitable as rootstocks to promote growth and precocity of clones is lacking. Six cocoa varieties were used to generate 36 clones for evaluation. For each of the six scions selected as ortets from previous progeny trials, the corresponding seed-derived family was regenerated through manual pollinations. The scions were grafted in a full diallel fashion on each of the six seed-derived family, and field evaluated in four replications following a randomized complete block design. Also, seed-derived varieties were evaluated in the nursery for vigour characteristics, and under similar conditions as the 36 clones in the field. Genotype heterozygosity at 72 SNP loci was significantly correlated with vigour characteristics of the six varieties. Seed-derived varieties of high vigour benefited clone performance for vigour, and dry bean yield when used as rootstocks but not as scion. The maximum photochemical efficiency of photosystem II (Fv/Fm) was different among seed-derived varieties and clones in the dry season but not the rainy season. Among clones, higher Fv/Fm values were associated with higher-vigour rootstock varieties. Diallel grafting model enabled partitioning of total variance into several sub-components. The variance due to specific compatibility ability was not significant for any of the measured traits. Decomposition of the reciprocal variance revealed that significant reciprocal effects were mainly due to significant maternal (rootstock) effects. The key finding is that, for any scion, the most precocious clone is obtained by grafting on high vigour rootstock varieties.</p></div>","PeriodicalId":37938,"journal":{"name":"Ecological Genetics and Genomics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140781796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-23DOI: 10.1016/j.egg.2024.100248
A. Kumar, A.K. Gupta, R.K. Lal
The bulk of senna plant components are used to make herbal medications to treat infections and other ailments. Except for grading, more than 80% of dried leaves are exported from India. The current study examined the genetic variability, correlations, and character contributions of 25 senna accessions. The amplitude of the GCV was larger than that of the PCV, showing that the selected factors interact genetically. X1 had substantial and positive connections with X4, X5, X6, X8, X11, and X13, while X2 had significant and positive links with X7, X10, X11, X12, X13, and X14. The characteristic X11 made the largest direct contribution to the X14 attribute. In X1 and X13, the indirect effect was greater. Fresh and dry leaf weight had high heritability and medium GA over the mean, respectively, whereas pod weight had high heritability and GA over the mean, at 93.62 and 94.93%. The mean, heritability, and GA over the mean of genotypes Gen 1, 2, 3, 4, and 25 were selected for high yield in the most economically significant traits. As a result, the senna genotypes mentioned above may be effectively exploited.
{"title":"Recent advances in the genetic parameters and prospects of cultivation of the climate-smart herb senna (Senna alexandrina Mill.): A significant industrial medicinal crop","authors":"A. Kumar, A.K. Gupta, R.K. Lal","doi":"10.1016/j.egg.2024.100248","DOIUrl":"https://doi.org/10.1016/j.egg.2024.100248","url":null,"abstract":"<div><p>The bulk of senna plant components are used to make herbal medications to treat infections and other ailments. Except for grading, more than 80% of dried leaves are exported from India. The current study examined the genetic variability, correlations, and character contributions of 25 senna accessions. The amplitude of the GCV was larger than that of the PCV, showing that the selected factors interact genetically. X1 had substantial and positive connections with X4, X5, X6, X8, X11, and X13, while X2 had significant and positive links with X7, X10, X11, X12, X13, and X14. The characteristic X11 made the largest direct contribution to the X14 attribute. In X1 and X13, the indirect effect was greater. Fresh and dry leaf weight had high heritability and medium GA over the mean, respectively, whereas pod weight had high heritability and GA over the mean, at 93.62 and 94.93%. The mean, heritability, and GA over the mean of genotypes Gen 1, 2, 3, 4, and 25 were selected for high yield in the most economically significant traits. As a result, the senna genotypes mentioned above may be effectively exploited.</p></div>","PeriodicalId":37938,"journal":{"name":"Ecological Genetics and Genomics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140646609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-22DOI: 10.1016/j.egg.2024.100245
Muhammad Nawaz , Irfanullah Khan , Farman Ullah Dawar , Ibni Amin Khalil , Zahid Hussain , Jan Saad , Syed Majid Rasheed , Khan Rahamdad
Aphid infestations pose a significant threat to wheat production worldwide, leading to substantial yield losses. Understanding the variation in wheat genotype response to aphids’ infestation across different sowing dates is crucial for effective insect pest management approaches. In this study, we investigated the relative resistance of various wheat genotypes to aphid infestations under varying sowing dates over multiple growing seasons 2019–20 and 2020–21 at Cereal Crops Research Institute, Pirsabak, Nowshera, Pakistan. The objectives were to assess the impact of sowing date on aphid infestations and to identify wheat genotypes that exhibit consistent resistance to aphids. The results revealed significant variations in aphid infestations across different sowing dates, with early-sown (October) wheat genotypes; Khaista-17 and PR-123 having 5.53 and 5.22 aphids/tiller, respectively, experiencing higher aphid pressure among all sowing dates, while lower aphid infestation 1.91 and 2.30 aphids/tiller observed on PR-125 and PR-127 respectively in late-sown (January). In contrast, Khaista-17 and PR-126 sown in October attained higher yield (4041 and 3639 kg ha−1), while the same genotypes attained 448 and 441 kg ha−1 respectively, that late-sown at January 2019–20. Similarly, these genotypes, Khaista-17 and PR-131 with 4.44 and 4.31 aphids/tiller not only sustained lower aphid populations but also demonstrated minimal yield loss due to aphid damage during 2020–21. This research highlights the importance of considering sowing date as a critical factor in wheat-aphid interactions. Furthermore, this study offers valuable insights for pest management strategies aimed at mitigating aphid-related yield losses and improve overall wheat production sustainability.
{"title":"Comparative resistance of wheat genotypes to aphid infestation under varied sowing dates","authors":"Muhammad Nawaz , Irfanullah Khan , Farman Ullah Dawar , Ibni Amin Khalil , Zahid Hussain , Jan Saad , Syed Majid Rasheed , Khan Rahamdad","doi":"10.1016/j.egg.2024.100245","DOIUrl":"https://doi.org/10.1016/j.egg.2024.100245","url":null,"abstract":"<div><p>Aphid infestations pose a significant threat to wheat production worldwide, leading to substantial yield losses. Understanding the variation in wheat genotype response to aphids’ infestation across different sowing dates is crucial for effective insect pest management approaches. In this study, we investigated the relative resistance of various wheat genotypes to aphid infestations under varying sowing dates over multiple growing seasons 2019–20 and 2020–21 at Cereal Crops Research Institute, Pirsabak, Nowshera, Pakistan. The objectives were to assess the impact of sowing date on aphid infestations and to identify wheat genotypes that exhibit consistent resistance to aphids. The results revealed significant variations in aphid infestations across different sowing dates, with early-sown (October) wheat genotypes; Khaista-17 and PR-123 having 5.53 and 5.22 aphids/tiller, respectively, experiencing higher aphid pressure among all sowing dates, while lower aphid infestation 1.91 and 2.30 aphids/tiller observed on PR-125 and PR-127 respectively in late-sown (January). In contrast, Khaista-17 and PR-126 sown in October attained higher yield (4041 and 3639 kg ha<sup>−1</sup>), while the same genotypes attained 448 and 441 kg ha<sup>−1</sup> respectively, that late-sown at January 2019–20. Similarly, these genotypes, Khaista-17 and PR-131 with 4.44 and 4.31 aphids/tiller not only sustained lower aphid populations but also demonstrated minimal yield loss due to aphid damage during 2020–21. This research highlights the importance of considering sowing date as a critical factor in wheat-aphid interactions. Furthermore, this study offers valuable insights for pest management strategies aimed at mitigating aphid-related yield losses and improve overall wheat production sustainability.</p></div>","PeriodicalId":37938,"journal":{"name":"Ecological Genetics and Genomics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140646608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The conservation of animal genetic resources guarantees the continual existence of diversified nature and thereby ensures the achievement of valuable economic, ecological, social, and cultural demands. Animal genetic resources can be conserved in their natural environments (in-situ conservation) and outside their natural habitats (ex-situ conservation) through different techniques such as zoo, captive breeding, aquarium, and in gene banks by collecting and preserving their germplasm. Reproductive technologies and cryopreservation of genetic materials play a pivotal role in preserving genetic diversity, supporting breeding programs, contributing to biodiversity conservation, adapting to changing environments, and fostering advancements in agriculture and animal husbandry. Its importance extends to ensuring food security, resilience in the face of climate change, and economic opportunities for communities. The collection and cryopreservation of genetic materials, including sperm, oocytes, embryos, and other biological materials capable of generating new offspring, have critical importance in supporting in-situ conservation efforts of threatened animals and the rehabilitation of extinct species and breeds. Advancements in reproductive technologies such as artificial insemination, in-vitro fertilization, embryo transfer, and nuclear transfer have greatly promoted efficiencies in animal reproduction and serve as a significant tool for domestic and wildlife animal conservation, particularly those threatened with extinction. Cryopreservation of germplasm and the application of reproductive technologies possess great success in breeding improvements and genetic resource conservation strategies, increasing the possibility of population rehabilitation. Good achievements have been observed in reconstituting animal populations in different parts of the world, while successful attempts have also been recorded in recovering extinct animals from cryopreserved genetic materials. In Ethiopia, reproductive technologies, such as cryopreservation and artificial insemination, are limited to the collection and distribution of bovine semen for genetic improvement and/or restocking activity. However, other reproductive technologies, including in vitro fertilization, ovulation induction, and reproductive surgeries, are not yet fully implemented due to various limitations. Initiative efforts are started in semen collection from indigenous cattle breeds, horses, and the endangered Ethiopian wolf for the conservation of the animal species or breed. Therefore, animal conservation strategies should adopt and benefit from advances in animal reproduction and cryopreservation technologies. Furthermore, proper emphasis and attention should be given to the development and use of cryopreservation and reproductive technologies in the conservation of animal genetic resources. Therefore, the purpose of this review is to emphasize the commonly utilized reproductive technologies
{"title":"The Role of Reproductive Technologies and Cryopreservation of Genetic Materials in the Conservation of Animal Genetic Resources","authors":"Aweke Engdawork, Teklewold Belayhun, Tesfalem Aseged","doi":"10.1016/j.egg.2024.100250","DOIUrl":"https://doi.org/10.1016/j.egg.2024.100250","url":null,"abstract":"<div><p>The conservation of animal genetic resources guarantees the continual existence of diversified nature and thereby ensures the achievement of valuable economic, ecological, social, and cultural demands. Animal genetic resources can be conserved in their natural environments (in-situ conservation) and outside their natural habitats (ex-situ conservation) through different techniques such as zoo, captive breeding, aquarium, and in gene banks by collecting and preserving their germplasm. Reproductive technologies and cryopreservation of genetic materials play a pivotal role in preserving genetic diversity, supporting breeding programs, contributing to biodiversity conservation, adapting to changing environments, and fostering advancements in agriculture and animal husbandry. Its importance extends to ensuring food security, resilience in the face of climate change, and economic opportunities for communities. The collection and cryopreservation of genetic materials, including sperm, oocytes, embryos, and other biological materials capable of generating new offspring, have critical importance in supporting in-situ conservation efforts of threatened animals and the rehabilitation of extinct species and breeds. Advancements in reproductive technologies such as artificial insemination, in-vitro fertilization, embryo transfer, and nuclear transfer have greatly promoted efficiencies in animal reproduction and serve as a significant tool for domestic and wildlife animal conservation, particularly those threatened with extinction. Cryopreservation of germplasm and the application of reproductive technologies possess great success in breeding improvements and genetic resource conservation strategies, increasing the possibility of population rehabilitation. Good achievements have been observed in reconstituting animal populations in different parts of the world, while successful attempts have also been recorded in recovering extinct animals from cryopreserved genetic materials. In Ethiopia, reproductive technologies, such as cryopreservation and artificial insemination, are limited to the collection and distribution of bovine semen for genetic improvement and/or restocking activity. However, other reproductive technologies, including in vitro fertilization, ovulation induction, and reproductive surgeries, are not yet fully implemented due to various limitations. Initiative efforts are started in semen collection from indigenous cattle breeds, horses, and the endangered Ethiopian wolf for the conservation of the animal species or breed. Therefore, animal conservation strategies should adopt and benefit from advances in animal reproduction and cryopreservation technologies. Furthermore, proper emphasis and attention should be given to the development and use of cryopreservation and reproductive technologies in the conservation of animal genetic resources. Therefore, the purpose of this review is to emphasize the commonly utilized reproductive technologies","PeriodicalId":37938,"journal":{"name":"Ecological Genetics and Genomics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140646882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Basil leaves are used to make a tea that promises to assist with vomiting, rheumatic pain, and loose motions. It includes a high concentration of antioxidants, which may increase longevity and health. The current study aims to assess the consistency, stability, and recommendations for producing essential oils from commercial genotypes and cultivars. To investigate genotype stability, field experiments were conducted in a semi-arid tropical climate in an RBD and replicated three times over three years in 2017, 2018, and 2019, at the CIMAP, RC, Hyderabad, south India. Each of the thirteen economic variables exhibited substantial diversity. In a three-year experiment, the stability of 20 basil genotypes was studied. There have been very few studies on the genotype and environmental interactions, stability, and diversity of basil. Nonetheless, no simultaneous assessments, stability, or reliability analyses have been performed in a multi-environment essential oil yield study on basil. According to the AMMI model, genotypes OC-6 and 8 for oil yield and OC-6 and 10 for linalool (%) were the most adaptable and stable varieties because they could withstand a wide range of environmental conditions. This leads to the conclusion that the most stable genotypes for linalool content (%) and essential oil production are OC-6, OC-8, and OC-10, all of which provide excellent yields. As a result, it is suggested that these genotypes and cultivars be commercialized.
{"title":"Stability pattern and simultaneous selection for the stable essential oil-yielding genotypes of basil","authors":"Ashish Kumar , R.K. Lal , Anil Kumar Gupta , Niranjan Kumar Arigari , C.S. Chanotiya","doi":"10.1016/j.egg.2024.100246","DOIUrl":"https://doi.org/10.1016/j.egg.2024.100246","url":null,"abstract":"<div><p>Basil leaves are used to make a tea that promises to assist with vomiting, rheumatic pain, and loose motions. It includes a high concentration of antioxidants, which may increase longevity and health. The current study aims to assess the consistency, stability, and recommendations for producing essential oils from commercial genotypes and cultivars. To investigate genotype stability, field experiments were conducted in a semi-arid tropical climate in an RBD and replicated three times over three years in 2017, 2018, and 2019, at the CIMAP, RC, Hyderabad, south India. Each of the thirteen economic variables exhibited substantial diversity. In a three-year experiment, the stability of 20 basil genotypes was studied. There have been very few studies on the genotype and environmental interactions, stability, and diversity of basil. Nonetheless, no simultaneous assessments, stability, or reliability analyses have been performed in a multi-environment essential oil yield study on basil. According to the AMMI model, genotypes OC-6 and 8 for oil yield and OC-6 and 10 for linalool (%) were the most adaptable and stable varieties because they could withstand a wide range of environmental conditions. This leads to the conclusion that the most stable genotypes for linalool content (%) and essential oil production are OC-6, OC-8, and OC-10, all of which provide excellent yields. As a result, it is suggested that these genotypes and cultivars be commercialized.</p></div>","PeriodicalId":37938,"journal":{"name":"Ecological Genetics and Genomics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140619306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Electrolyzed water is expected to have a sterilizing effect on bacteria and viruses, which makes it useful for hygiene management, such as cleaning dishes or medical equipment. Electrolyzed water treatment may affect the cellular structure of microbes, leading to DNA leakage or degradation. Here, we conducted a next-generation sequencing (NGS) analysis of bacterial diversity in washing solution to elucidate the impact of cleaning food surfaces with electrolyzed water. Modified primers were used to evaluate the differences between the existing primers used to obtain NGS sequences and through statistical analysis. The NGS reads obtained using the modified primers tended to reveal more Enterobacteriaceae sequences, and fewer mitochondrial sequences than did those obtained with conventional primers. An UniFrac distance analysis and Nonmetric multidimensional scaling (NMDS) revealed that the results obtained with the modified primer set made the differences between samples more distinct. Real-time polymerase chain reaction (PCR) using the template DNA obtained from an electrolyzed water or sodium hypochlorite solution wash revealed that some of the DNA could not be amplified using PCR of the 16S rRNA genes suggesting that the DNA fragmentation had occurred due to the electrolyzed water treatment. Our results revealed that the bacterial species removed from the food surfaces varied depending on the washing treatment. Electrolyzed water treatment may be advantageous for removing Enterobacteriaceae from leafy vegetables.
电解水可望对细菌和病毒产生杀菌作用,因此可用于卫生管理,如清洗餐具或医疗设备。电解水处理可能会影响微生物的细胞结构,导致 DNA 泄漏或降解。在此,我们对清洗液中的细菌多样性进行了新一代测序(NGS)分析,以阐明用电解水清洗食品表面的影响。我们使用改良引物来评估现有引物之间的差异,并通过统计分析获得 NGS 序列。与使用传统引物获得的序列相比,使用改良引物获得的 NGS 读数倾向于显示更多的肠杆菌科序列和更少的线粒体序列。UniFrac 距离分析和非度量多维标度(NMDS)显示,使用改良引物组获得的结果使样本之间的差异更加明显。使用从电解水或次氯酸钠溶液洗涤液中获得的模板 DNA 进行实时聚合酶链反应(PCR)发现,部分 DNA 无法通过 16S rRNA 基因的 PCR 扩增,这表明电解水处理导致了 DNA 断裂。我们的研究结果表明,从食物表面清除的细菌种类因清洗处理方法而异。电解水处理可能有利于去除叶菜中的肠杆菌科细菌。
{"title":"Impact of electrolyzed water treatment on bacterial communities in food washing processes","authors":"Akifumi Hosoda , Yuka Ito , Takaaki Kojima , Yki Ogata , Minami Haga , Yu Akimoto , Miki Shirasawa , Michiru Kishimoto","doi":"10.1016/j.egg.2024.100244","DOIUrl":"https://doi.org/10.1016/j.egg.2024.100244","url":null,"abstract":"<div><p>Electrolyzed water is expected to have a sterilizing effect on bacteria and viruses, which makes it useful for hygiene management, such as cleaning dishes or medical equipment. Electrolyzed water treatment may affect the cellular structure of microbes, leading to DNA leakage or degradation. Here, we conducted a next-generation sequencing (NGS) analysis of bacterial diversity in washing solution to elucidate the impact of cleaning food surfaces with electrolyzed water. Modified primers were used to evaluate the differences between the existing primers used to obtain NGS sequences and through statistical analysis. The NGS reads obtained using the modified primers tended to reveal more Enterobacteriaceae sequences, and fewer mitochondrial sequences than did those obtained with conventional primers. An UniFrac distance analysis and Nonmetric multidimensional scaling (NMDS) revealed that the results obtained with the modified primer set made the differences between samples more distinct. Real-time polymerase chain reaction (PCR) using the template DNA obtained from an electrolyzed water or sodium hypochlorite solution wash revealed that some of the DNA could not be amplified using PCR of the 16S rRNA genes suggesting that the DNA fragmentation had occurred due to the electrolyzed water treatment. Our results revealed that the bacterial species removed from the food surfaces varied depending on the washing treatment. Electrolyzed water treatment may be advantageous for removing Enterobacteriaceae from leafy vegetables.</p></div>","PeriodicalId":37938,"journal":{"name":"Ecological Genetics and Genomics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140619307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-15DOI: 10.1016/j.egg.2024.100247
Sushil Kumar Patel , R.K. Lal , J.C. Benjamin , V.R. Singh , C.S. Chanotiya , D. Bisht , A. Kumar
Chile has been used as a spice because of its flavor and zest, which complement the minerals, vitamins, and other elements it contains when cooked. The objective of the current study was to determine how P. fluorescens and S. marcescens promote plant growth in Chili plants. The PGPR's phosphate solubilizing, IAA, NH3, HCN production, and antifungal activities were ascertained using in vitro experiments. During the study period 2020–2022, the experiment was conducted using a completely randomized block design, with three replications of the three treatments for each microorganism, as follows: 1) P. fluorescens: T0 (control, unsterile soil), T1 (P. fluorescens in rice husk), and T2 (P. fluorescens in sawdust); 2) S. marcescens: T0 (control, unsterile soil), T1 (S. marcescens in rice husk), and T2 (S. marcescens in sawdust) with no fertilizer. After seeding, the chili plant's parameters were measured at 15, 30, 45, 60, and 75 days and compared to the control using four traits: plant height, root length, shoot length, and the number of leaves per plant. The following bio-formulation, employing T0, enhanced plant height growth after seeding: In rice husk, Days 75 (B2 × D5 × T2) = 21.20 for S. marcescens > Days 75 (B1 × D5 × T2) = 20.83 for P. fluorescens > Days 60 (B1 × D4 × T2) = 19.17 for P. fluorescens Days 75 (B2 × D5 × T0) for S. marcescens in control, days 60 (B1 × D4 × T1 (16.43 cm) for P. fluorescens in sawdust, and days 75 (B1 × D5 × T0) for P. fluorescens in control. The root length (cm) for P. fluorescens in sawdust is 7.23 days (B1 × D5 × T2), which is more than P. marcescens in sawdust (days 60 B1 × D4 × T2), P. fluorescens in control (days 75 (B1 × D5 × T0) = 6.60 cm), and S. marcescens in control (days 75 (B2 × D5 × T0) = 6.60 cm), etc. Rice husk and sawdust were used to create the bio-formulations. The maximum plant growth was achieved at 75 days when S. marcescens was grown in a sawdust medium, including sawdust as a carbon source and carboxymethyl cellulose as a carrier source. When the different compositions of the bio-formulation were tested on chili plants, it was found that P. fluorescens and S. marcescens in sawdust bio-formulation were more effective.
智利因其味道鲜美、风味独特而被用作香料,在烹饪时与所含的矿物质、维生素和其他元素相得益彰。本研究的目的是确定 P. fluorescens 和 S. marcescens 如何促进辣椒植物的生长。通过体外实验确定了 PGPR 的磷酸盐溶解、IAA、NH3、HCN 生产和抗真菌活性。在 2020-2022 年研究期间,实验采用完全随机区组设计,每种微生物的三个处理有三次重复,具体如下:1) P. fluorescens:T0(对照组,无菌土壤)、T1(稻壳中的荧光蛋白)和 T2(锯屑中的荧光蛋白);2)S. marcescens:T0(对照组,无菌土壤)、T1(稻壳中的 S.marcescens)和 T2(锯屑中的 S.marcescens),不施肥。播种后,分别在 15 天、30 天、45 天、60 天和 75 天测量辣椒植株的各项参数,并通过植株高度、根长、芽长和每株叶片数这四个性状与对照进行比较。以下生物制剂采用 T0,提高了播种后的株高生长:在稻壳中,S. marcescens 的第 75 天(B2 × D5 × T2)= 21.20;P. fluorescens 的第 75 天(B1 × D5 × T2)= 20.83;P. marcescens 的第 60 天(B1 × D4 × T2)= 19.17。对照组中 S. marcescens 的第 75 天(B2 × D5 × T0),锯屑中 P. fluorescens 的第 60 天(B1 × D4 × T1)(16.43 厘米),以及对照组中 P. fluorescens 的第 75 天(B1 × D5 × T0)。锯末中的 P. fluorescens 的根长(厘米)为 7.23 天(B1 × D5 × T2),比锯末中的 P. marcescens(60 天 B1 × D4 × T2)、对照中的 P. fluorescens(75 天(B1 × D5 × T0)= 6.60 厘米)和对照中的 S. marcescens(75 天(B2 × D5 × T0)= 6.60 厘米)等要长。稻壳和锯末被用来制作生物配方。当 S. marcescens 在锯屑培养基(包括作为碳源的锯屑和作为载体源的羧甲基纤维素)中生长 75 天时,植物生长达到最大值。在辣椒植株上测试生物制剂的不同成分时发现,锯屑生物制剂中的 P. fluorescens 和 S. marcescens 效果更好。
{"title":"Plant-growth stimulating bacteria × environments/bio-formulations interactions on the growth-promoting activities of Serratia marcescens and Pseudomonas fluorescens on chili","authors":"Sushil Kumar Patel , R.K. Lal , J.C. Benjamin , V.R. Singh , C.S. Chanotiya , D. Bisht , A. Kumar","doi":"10.1016/j.egg.2024.100247","DOIUrl":"https://doi.org/10.1016/j.egg.2024.100247","url":null,"abstract":"<div><p>Chile has been used as a spice because of its flavor and zest, which complement the minerals, vitamins, and other elements it contains when cooked. The objective of the current study was to determine how <em>P. fluorescens</em> and <em>S. marcescens</em> promote plant growth in Chili plants. The PGPR's phosphate solubilizing, IAA, NH3, HCN production, and antifungal activities were ascertained using in vitro experiments. During the study period 2020–2022, the experiment was conducted using a completely randomized block design, with three replications of the three treatments for each microorganism, as follows: 1) <em>P. fluorescens</em>: T0 (control, unsterile soil), T1 (<em>P. fluorescens</em> in rice husk), and T2 (<em>P. fluorescens</em> in sawdust); 2) <em>S. marcescens</em>: T0 (control, unsterile soil), T1 (<em>S. marcescens</em> in rice husk), and T2 (<em>S. marcescens</em> in sawdust) with no fertilizer. After seeding, the chili plant's parameters were measured at 15, 30, 45, 60, and 75 days and compared to the control using four traits: plant height, root length, shoot length, and the number of leaves per plant. The following bio-formulation, employing T0, enhanced plant height growth after seeding: In rice husk, Days 75 (B2 × D5 × T2) = 21.20 for <em>S. marcescens</em> > Days 75 (B1 × D5 × T2) = 20.83 for <em>P. fluorescens</em> > Days 60 (B1 × D4 × T2) = 19.17 for <em>P. fluorescens</em> Days 75 (B2 × D5 × T0) for <em>S. marcescens</em> in control, days 60 (B1 × D4 × T1 (16.43 cm) for <em>P. fluorescens</em> in sawdust, and days 75 (B1 × D5 × T0) for <em>P. fluorescens</em> in control. The root length (cm) for <em>P. fluorescens</em> in sawdust is 7.23 days (B1 × D5 × T2), which is more than <em>P. marcescens</em> in sawdust (days 60 B1 × D4 × T2), <em>P. fluorescens</em> in control (days 75 (B1 × D5 × T0) = 6.60 cm), and <em>S. marcescens</em> in control (days 75 (B2 × D5 × T0) = 6.60 cm), etc. Rice husk and sawdust were used to create the bio-formulations. The maximum plant growth was achieved at 75 days when <em>S. marcescens</em> was grown in a sawdust medium, including sawdust as a carbon source and carboxymethyl cellulose as a carrier source. When the different compositions of the bio-formulation were tested on chili plants, it was found that <em>P. fluorescens</em> and <em>S. marcescens</em> in sawdust bio-formulation were more effective.</p></div>","PeriodicalId":37938,"journal":{"name":"Ecological Genetics and Genomics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140558025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Reproductive traits of sheep are under the control of both the environment and genetics. Moreover, most of these traits have low heritability, are sex-limited, and expressed later in the animal’s life, which makes it difficult to quantify in young animals and to apply direct selection to the traits. This calls for the need to identify and include the genetic bases of fertility traits in selection decisions to enhance the performance of fertility traits in sheep breeding programs. Therefore, several polymorphisms in many genes associated with fertility traits have been reported in several sheep breeds worldwide. The bone morphogenetic protein 15 (BMP15), bone morphogenetic protein receptor 1B (BMPR1B), and growth differentiation factor 9 (GDF9) genes are the major fecundity genes investigated in several sheep breeds studied. Polymorphisms on these genes have shown significant associations with fertility traits such as ovulation rate and litter size and have been used in selection programs. However, research findings focusing on candidate genes affecting fertility traits in indigenous sheep breeds from developing countries are lacking. This review has shown that the genes and the polymorphisms on the genes are variable between and within breeds of the sheep explored thus far, which calls for further efforts to examine sheep breeds in developing countries.
{"title":"A review on candidate genes associated with sheep fertility traits: Implications for genetic improvement of indigenous sheep breeds in developing countries","authors":"Mezgebu Getaneh , Mengistie Taye , Kefyalew Alemayehu , Aynalem Haile , Tesfaye Getachew , Workneh Ayalew","doi":"10.1016/j.egg.2024.100243","DOIUrl":"https://doi.org/10.1016/j.egg.2024.100243","url":null,"abstract":"<div><p>Reproductive traits of sheep are under the control of both the environment and genetics. Moreover, most of these traits have low heritability, are sex-limited, and expressed later in the animal’s life, which makes it difficult to quantify in young animals and to apply direct selection to the traits. This calls for the need to identify and include the genetic bases of fertility traits in selection decisions to enhance the performance of fertility traits in sheep breeding programs. Therefore, several polymorphisms in many genes associated with fertility traits have been reported in several sheep breeds worldwide. The bone morphogenetic protein 15 (BMP15), bone morphogenetic protein receptor 1B (BMPR1B), and growth differentiation factor 9 (GDF9) genes are the major fecundity genes investigated in several sheep breeds studied. Polymorphisms on these genes have shown significant associations with fertility traits such as ovulation rate and litter size and have been used in selection programs. However, research findings focusing on candidate genes affecting fertility traits in indigenous sheep breeds from developing countries are lacking. This review has shown that the genes and the polymorphisms on the genes are variable between and within breeds of the sheep explored thus far, which calls for further efforts to examine sheep breeds in developing countries.</p></div>","PeriodicalId":37938,"journal":{"name":"Ecological Genetics and Genomics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140557558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-28DOI: 10.1016/j.egg.2024.100239
Ashish Kumar , Anil Kumar Gupta , Saba Siddiqui , A.C. Jnanesha , R.K. Lal , Mohammad Haris Siddiqui
The relationship between genotype and season significantly hinders the development of stable genotype selection and improved genotypes for senna breeding programs. This study aimed to assess stable, high-yielding senna genotypes under South Indian conditions. Twenty-five different senna genotypes were tested for yield stability using a randomized, completely-block design. The additive main effects and multiplicative interaction (AMMI) model was used to investigate the relationships between genotype (G), environment (E), and genotype × environment. Based on the findings, the three genotypes—GEN 1, GEN 9, and GEN 18—might be recommended for large-scale production. Stable and high-yielding genotypes should be the main genetic resources used in senna-based cropping season breeding programs.
{"title":"Reliable and consistent genotype selection for leaf biomass and natural sennosides yield across multi-environments in Senna (Senna alexandrina Mill)","authors":"Ashish Kumar , Anil Kumar Gupta , Saba Siddiqui , A.C. Jnanesha , R.K. Lal , Mohammad Haris Siddiqui","doi":"10.1016/j.egg.2024.100239","DOIUrl":"https://doi.org/10.1016/j.egg.2024.100239","url":null,"abstract":"<div><p>The relationship between genotype and season significantly hinders the development of stable genotype selection and improved genotypes for senna breeding programs. This study aimed to assess stable, high-yielding senna genotypes under South Indian conditions. Twenty-five different senna genotypes were tested for yield stability using a randomized, completely-block design. The additive main effects and multiplicative interaction (AMMI) model was used to investigate the relationships between genotype (G), environment (E), and genotype × environment. Based on the findings, the three genotypes—GEN 1, GEN 9, and GEN 18—might be recommended for large-scale production. Stable and high-yielding genotypes should be the main genetic resources used in senna-based cropping season breeding programs.</p></div>","PeriodicalId":37938,"journal":{"name":"Ecological Genetics and Genomics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140343816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}