Ecological Genetics and Genomics最新文献_第7页

Genetic diversity analysis in Cordia dichotoma from Himalayan foothills and plains of north India using ISSR markers 利用ISSR标记分析喜马拉雅山麓和印度北部平原科迪亚的遗传多样性

Q3 Agricultural and Biological Sciences

Ecological Genetics and Genomics

Pub Date : 2025-09-01 Epub Date: 2025-06-28 DOI: 10.1016/j.egg.2025.100380

Atul Chamyal , Navpreet Parmar , Shubham Chauhan , Nishant Chauhan , Rakesh Kumar , Pitamber Dutt Sharma , Vikas Sharma

Cordia dichotoma is an important member of the family Boraginaceae. Its fruits, leaves and flowers are edible and consumed by many communities in India. It is an underutilized plant that exhibits many health benefits and is used as therapeutic diets. However, the natural populations of this plant species have decreased drastically. In this research work, germplasms of C. dichotoma have been explored for genetic diversity and population structure using Inter-Simple Sequence Repeat (ISSR) markers. Sixty accessions of this species were collected from the Himalayan foothills of Himachal Pradesh and plains of Punjab, and characterized using ISSR markers. In total, 10 ISSR primers amplified 72 polymorphic fragments, with an average of 7.2 fragments. The size of the amplified fragments ranged from 250 to 2000 bp. The primer UBC-810 amplified a minimum of 4 fragments, while UBC-807 amplified the maximum (12) fragments. The PIC values ranged from 0.32 (UBC-848) to 0.49 (UBC-808, UBC-812, UBC-834), with an average of 0.44. The marker index was lowest (1.82) for primer UBC-810 and highest (5.11) for primer UBC-807, with a mean value of 3.17. Nei's gene diversity (0.268) and Shannon's diversity index (0.399) were highest in the Hoshiarpur population. Genetic structure revealed the presence of three gene pools within the studied accessions and Dendrogram showed clear two groups. The results of the present study indicated that selected accessions such as K8, H4, H7, H10, M21 and M62 can be used for breeding, conservation and improvement of C. dichotoma germplasm for sustainable utilization of this underutilized and neglected species.

Cordia dichotoma是Boraginaceae科的重要成员。它的果实、叶子和花在印度的许多社区都是可食用的。它是一种未被充分利用的植物，显示出许多健康益处，并被用作治疗性饮食。然而，这种植物的自然种群数量急剧减少。本研究利用ISSR （Inter-Simple Sequence Repeat，简单序列重复）标记，对双歧花种质资源的遗传多样性和群体结构进行了研究。在喜马偕尔邦喜马拉雅山麓和旁遮普邦平原采集了60份本种，利用ISSR标记对其进行了鉴定。10条ISSR引物共扩增出72条多态性片段，平均扩增7.2条。扩增片段的大小在250 ~ 2000 bp之间。引物UBC-810扩增最少4个片段，而UBC-807扩增最多12个片段。PIC值为0.32 (UBC-848) ~ 0.49 (UBC-808、UBC-812、UBC-834)，平均值为0.44。引物UBC-810的标记指数最低（1.82），引物UBC-807的标记指数最高（5.11），平均值为3.17。Hoshiarpur群体的Nei’s基因多样性指数和Shannon’s多样性指数最高，分别为0.268和0.399。遗传结构显示出3个基因库，树形图显示出明显的2个类群。本研究结果表明，K8、H4、H7、H10、M21和M62等选育材料可用于二裂木种质资源的培育、保护和改良，使二裂木种质资源得到可持续利用。

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引用次数: 0

Evaluation of genetic variability in neem (Azadirachta indica) germplasm for azadirachtin yield across peninsular and central India 印楝（印楝）种质对印楝素产量的遗传变异评价

Q3 Agricultural and Biological Sciences

Ecological Genetics and Genomics

Pub Date : 2025-09-01 Epub Date: 2025-05-23 DOI: 10.1016/j.egg.2025.100371

K. Rajarajan, Shreishtha Singh, A. Arunachalam, A.K. Handa

Azadirachtin, produced from neem, is a broad-spectrum, eco-friendly biopesticide properties. Enhancing neem genotypes for higher azadirachtin content can significantly improve the sustainability and efficiency of biopesticide production. This study evaluated 90 neem germplasms for seed morphological and biochemical traits to assess genetic variability and identify superior genotypes for azadirachtin improvement. A randomized block design (RBD) was employed for plantation. Traits such as seed length, seed breadth, seed kernel ratio, oil content, and azadirachtin concentration were measured. Substantial variation was observed across traits, indicating a broad genetic base. Seed length (10.17–17.10 mm), seed width (5.14–7.50 mm), and 100-seed weight (8.89–20.43 g) showed wide ranges. Seed kernel ratio ranged from 1.50 to 4.50, oil content from 11 % to 48 %, and azadirachtin concentration (AZC) from 148.85 to 2850.14 mg/kg, highlighting significant variability. The south-eastern (SE) population exhibited superior seed traits and AZC compared to others. Correlation analysis showed moderate positive associations between seed traits (length, width, and weight) and AZC, suggesting potential for indirect selection. Path analysis confirmed the direct contribution of seed width and weight to AZC. High heritability and genetic advance in AZC and oil content indicate additive gene action and suitability for selection. Principal component analysis identified seed length, width, and weight as primary contributors to variation (56.94 %). Five elite accessions (VKAF-75, VKAF-59, VKAF-58, VKAF-53, and VKAF-139) with high azadirachtin yield were identified, suitable for breeding. The findings offers the importance of seed traits and the potential of SE germplasms as donor parents for future breeding programs.

印楝素是从印度楝树中提取的，是一种广谱、环保的生物农药。通过改良印楝基因型，提高印楝素含量，可显著提高生物农药生产的可持续性和效率。本研究对90份印楝种子形态和生化性状进行了评价，以评估遗传变异，并鉴定出改良印楝素的优良基因型。人工林采用随机区组设计（RBD）。测定了种子长度、种子宽度、籽粒比、含油量、印楝素浓度等性状。在性状上观察到大量的变异，表明有广泛的遗传基础。种子长（10.17 ~ 17.10 mm）、种子宽（5.14 ~ 7.50 mm）、百粒重（8.89 ~ 20.43 g）变化幅度较大。籽粒比为1.50 ~ 4.50，含油量为11% ~ 48%，印楝素浓度（AZC）为148.85 ~ 2850.14 mg/kg，差异显著。东南居群的种子性状和AZC均优于其他居群。相关分析表明，种子性状（长、宽、重）与AZC呈中等正相关，表明存在间接选择的可能。通径分析证实了种子宽和种子重对AZC的直接贡献。AZC和含油量的高遗传率和遗传先进性表明加性基因的作用和选择的适宜性。主成分分析发现种子长度、宽度和重量是变异的主要影响因子（56.94%）。鉴定出5个优良的印楝素高产材料（VKAF-75、VKAF-59、VKAF-58、VKAF-53和VKAF-139），适合育种。这一发现提供了种子性状的重要性和SE种质作为供体亲本在未来育种计划中的潜力。

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引用次数: 0

Multivariate analyses, heritability, and genotype environment interaction of dekoko (pisum sativum Var. abyssinicum) germplasms in tigray, northern Ethiopia 埃塞俄比亚北部tigray地区dekoko （pisum sativum Var. abyssinicum）种质资源的多变量分析、遗传力和基因型环境互作

Q3 Agricultural and Biological Sciences

Ecological Genetics and Genomics

Pub Date : 2025-09-01 Epub Date: 2025-05-28 DOI: 10.1016/j.egg.2025.100374

Gebru Equar Gebremichael , Yemane Tsehaye Baryatsion , Fetien Abay Abera , Yemane G.Egziabher , Desta Berhe Sbhatu , Genet Atsbeha

Dekoko (Pisum sativum var. abyssinicum) is native to Ethiopia and is known for its high nutritional value and unique flavor. Investigating the genetic variability, heritability, and genotype-environment interaction of Dekoko is essential for understanding its potential for crop improvement and adaptation to changing environmental conditions. Field-based phenotyping of 17 agro-morphological traits was used to assess the extent of genetic variability, heritability and genotype-environment interaction in 120 Dekoko germplasms. The experiment was laid at alpha lattice design with two replications, at Habes, Atsela, Bolonta, and H. burda experimental sites in two growing seasons. As a result, substantial genetic variation in phenological, vegetative, and yield-related traits was observed in the studied germplasms. High heritability was observed for days to 50 % emergence (86.69 %), seed length (85.70 %), seed width (76.67 %), and days to 50 % maturity (65.40 %). AMMI biplot models analysis revealed that the environment was the dominant factor (98.01 %), followed by genotypes (1.60 %) and genotype environment interaction (0.39). Genotypes G65, G46, and G73 exhibited stability, while G23, G33, G27, G62, G58, and G49 demonstrated adaptability across diverse environmental conditions. Additionally, GGE analysisbased on yield performance identified environmental classification and categorized Atsela_2019 Bolonta_2019, Atsela_2020 and H. Burda_2020 as favorable environments for majority of the genotypes. The results indicate that certain genotypes maintain consistent performance across different locations, emphasizing the need to select appropriate varieties based on environmental conditions to optimize grain yield potential. These findings contribute to breeding programs focused on improving yield stability and adaptability in P. sativum var. abyssinicum.

Dekoko （Pisum sativum var. abyssinicum）原产于埃塞俄比亚，以其高营养价值和独特风味而闻名。研究Dekoko的遗传变异性、遗传力和基因型-环境相互作用对于了解其作物改良和适应不断变化的环境条件的潜力至关重要。采用17个农业形态性状的田间分型方法，对120份德科科种质资源的遗传变异程度、遗传力和基因型-环境互作进行了评价。实验采用α晶格设计，在两个生长季节分别在Habes、Atsela、Bolonta和H. burda试验点进行两次重复。结果，在研究的种质中观察到物候、营养和产量相关性状的大量遗传变异。出苗期至50%（86.69%）、种子长度（85.70%）、种子宽度（76.67%）和成熟期至50%（65.40%）的遗传率较高。AMMI双图模型分析结果显示，环境因子为显性因子（98.01%），其次为基因型因子（1.60%）和基因型-环境互作因子（0.39%）。基因型G65、G46和G73表现出稳定性，而基因型G23、G33、G27、G62、G58和G49表现出对不同环境条件的适应性。此外，基于产量表现的GGE分析确定了环境分类，并将Atsela_2019 Bolonta_2019、Atsela_2020和H. Burda_2020归类为大多数基因型的有利环境。结果表明，某些基因型在不同地区保持一致的表现，强调需要根据环境条件选择合适的品种，以优化粮食产量潜力。这些发现有助于提高阿比西尼亚葡萄的产量稳定性和适应性。

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引用次数: 0

Unraveling genetic diversity in Arctium lappa L. populations from the north-west Himalaya using RAPD and ISSR marker techniques 利用RAPD和ISSR标记技术揭示西北喜马拉雅牛蒡群体的遗传多样性

Q3 Agricultural and Biological Sciences

Ecological Genetics and Genomics

Pub Date : 2025-09-01 Epub Date: 2025-05-30 DOI: 10.1016/j.egg.2025.100376

Nusrat Fayaz Bhat , Vikas Sharma , Raghbir Chand Gupta

Arctium lappa, commonly known as Burdock or Jangli Kuth, is a medicinally significant plant species. It is traditionally used in herbal medicine for its anti-inflammatory, antioxidant, and hepatoprotective properties. In the present study, genetic variation and population structure among diverse populations of A. lappa, collected from a wide range of ecological zones across the Northwest Himalaya-, were evaluated using Random Amplified Polymorphic DNA (RAPD) and Inter Simple Sequence Repeat (ISSR). A total of 28 primers (11 RAPD and 17 ISSR) were employed, yielding 230 distinct, clear, and reproducible polymorphic bands 104 from RAPD and 126 from ISSR markers indicating substantial genetic variation across the populations. The combined marker data revealed a high average percentage of polymorphism (96.94%), suggesting broad genetic heterogeneity. Additionally, the average Polymorphic Information Content (PIC) and Marker Index (MI) values were 0.31 and 2.63, respectively. Cluster analysis based on the Jaccard similarity coefficient and UPGMA grouped all accessions into three major clusters, each predominantly representing different geographic regions with respect to altitudinal ranges of Jammu & Kashmir and Uttarakhand. Bayesian STRUCTURE analysis showed the presence of two genetically distinct populations, with considerable levels of admixture, indicating ongoing gene flow and genetic exchange among geographically separated populations. These findings underscore the extensive genetic variability within A. lappa and provide a valuable genetic baseline for the identification of elite germplasm. Such information is pivotal for the formulation of region-specific conservation strategies, efficient breeding programs, and the sustainable exploitation of this underutilized yet valuable medicinal species.

牛蒡，俗称牛蒡或牛蒡，是一种重要的药用植物。由于其抗炎、抗氧化和保护肝脏的特性，传统上它被用于草药中。采用RAPD （Random Amplified Polymorphic DNA）和ISSR （Inter Simple Sequence Repeat）技术，对喜玛拉雅西北缘不同种群间的遗传变异和种群结构进行了分析。共使用28条引物（11条RAPD标记和17条ISSR标记），得到230条明显、清晰、可复制的多态性条带，其中104条来自RAPD标记，126条来自ISSR标记，表明群体间存在显著的遗传变异。组合标记数据显示较高的平均多态性百分比（96.94%），表明存在广泛的遗传异质性。多态性信息含量（PIC）和标记指数（MI）的平均值分别为0.31和2.63。基于Jaccard相似系数和UPGMA的聚类分析将所有资料分为3个主要聚类，每个聚类主要代表查谟和安邦不同的地理区域。克什米尔和北阿坎德邦。贝叶斯结构分析表明，存在两个遗传上截然不同的群体，具有相当程度的混合，表明地理上分离的群体之间正在进行基因流动和遗传交换。这些发现强调了羊腹草广泛的遗传变异，为鉴定优质种质提供了有价值的遗传基线。这些信息对于制定特定区域的保护策略，有效的育种计划以及可持续开发这一未被充分利用但有价值的药用物种至关重要。

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引用次数: 0

Assessment of wheat genotypes performance grown in gypsum soil under two irrigation water sources 两种灌溉水源下石膏土中小麦基因型性能评价

Q3 Agricultural and Biological Sciences

Ecological Genetics and Genomics

Pub Date : 2025-09-01 Epub Date: 2025-08-12 DOI: 10.1016/j.egg.2025.100395

Waleed A.T. El-Fahdawi , Nawfal A. Sabry , Mohammed Hamdan Al-Issawi , Husam Munef Obiad , Jabbar Sh E. Al-Esawi

Two factorial field experiment was carried out during the fall season of 2023 at the agricultural field of the Euphrates Basin Development Center, Anbar University. To investigate the suitability of five wheat genotypes selected for cultivation in arid and semi-arid regions (under the environmental conditions of the western region of Iraq) in gypsum soils. The first factor involved two water sources, namely Euphrates water and well water. The second factor included five wheat genotypes i.e., G-6, G-19, G-20, G-30, G-36. The considered genotypes were recently introduced to Iraq to be adopted for cultivation in arid and semi-arid areas. The treatments were randomly arranged in Randomized Complete Block Design (RCBD) with three replications. The results showed that the growth and production of wheat genotypes have negatively affected by well water. However, well water can serve as a viable alternative if the right wheat genotype is used. Among all genotypes, G-30 was prominent and showed considerable performance under the effect of both water sources (Euphrates water and well water).

二因子田间试验于2023年秋季在安巴尔大学幼发拉底河流域开发中心农田进行。研究5种小麦基因型在干旱和半干旱地区（伊拉克西部地区环境条件下）石膏土中的适宜性。第一个因素涉及两个水源，即幼发拉底河水和井水。第二个因子包括5个小麦基因型G-6、G-19、G-20、G-30、G-36。所考虑的基因型最近被引入伊拉克，用于干旱和半干旱地区的种植。采用随机完全区组设计（RCBD），随机安排3个重复。结果表明，井水对小麦基因型的生长和生产均有不利影响。然而，如果使用正确的小麦基因型，井水可以作为一种可行的替代品。在所有基因型中，G-30表现突出，在两种水源（幼发拉底河水和井水）的影响下均表现优异。

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引用次数: 0

Genetic variability among fine flavor cocoa hybrids for precocity and tolerance to cocoa swollen shoot virus disease 优良风味可可杂交种早熟及对可可肿芽病毒病耐受性的遗传变异

Q3 Agricultural and Biological Sciences

Ecological Genetics and Genomics

Pub Date : 2025-09-01 Epub Date: 2025-08-04 DOI: 10.1016/j.egg.2025.100392

Abu Mustapha Dadzie, Ebenezer Obeng-Bio, Kwabena Asare Bediako, Maame Adjoa Segbefia, Atta Ofori, Frank Owusu-Ansah, Owusu Domfeh

Cocoa (Theobroma cacao L.) contributes significantly to gross domestic product (GDP) and livelihood of over 700,000 people in Ghana. The objectives of this study were to determine (1) precocity, vigor and breeding value of five fine flavor clones across two contrasting agro-ecologies, (2) gene action modulating seven agronomic traits including cocoa swollen shoot disease (CSSD) tolerance. Thirty F₁ families were generated by crossing six superior females with five fine flavor clones in a 6 × 5 North Carolina design II (NCII). Two sets of experiments were conducted; field evaluation of 30 hybrids with two checks across Tafo and Afosu while the second experiment involved a gauze house screening of same set of hybrids for CSSD tolerance. Results indicated significant (P < 0.05) variability among the hybrids for the studied traits attributable to location and genotype. Most precocious clone with significant contribution to pod number was SGU50, while most precocious hybrids included CRG9006 × SGU50 and GU144C × SGU50. Clone GU144C had significant and positive general combining ability effect for vigor. Best surviving hybrids across locations included CRG9006 × ICS1, CRG9006 × SGU50, MAN15-2 × PLAYA-ALTA2 and MAN15-2 × SGU50. Field survival of hybrids depended on location and hybrid type.

Hybrids were sensitive to the New Juabeng strain of the cacao swollen shoot Togo B virus (CSSTBV) species. Severity of (CSSD) symptom expression was highly variable. Five hybrids, (CRG9006 × CC11, MAN15-2 × CC11, CRG9006 × PLAYA-ALTA2, PA7 × ICS1, PA150 × VENC4-4) were tolerant to CSSD. Most studied traits were conditioned by additive and non-additive gene actions, therefore, both recurrent and reciprocal recurrent selection approaches would be effective breeding strategies for improvement.

可可（Theobroma cacao L.）对加纳70多万人的国内生产总值和生计做出了重大贡献。本研究的目的是确定(1)不同农业生态条件下5个优质风味无性系的早熟性、活力和育种价值；(2)可可肿芽病（CSSD）耐受性等7个农艺性状的基因调控作用。以6 × 5北卡罗莱纳设计II （NCII）为材料，将6个优良雌性与5个优良风味无性系杂交，得到30个F1家族。进行了两组实验；现场评价了30个杂交种，在Tafo和Afosu进行了两次检查，而第二次试验涉及对同一组杂交种的CSSD耐受性进行了纱布筛选。结果显示显著(P <；所研究性状在杂交种间因位置和基因型的差异（0.05）。对荚果数贡献最大的早熟无性系为SGU50，而对荚果数贡献最大的早熟杂交种为CRG9006 × SGU50和GU144C × SGU50。无性系GU144C具有显著的正一般配合力效应。在不同地点存活最好的杂交品种包括CRG9006 × ICS1、CRG9006 × SGU50、MAN15-2 × PLAYA-ALTA2和MAN15-2 × SGU50。杂交种的田间存活率取决于地点和杂交种。杂交种对可可膨化芽多哥B病毒（CSSTBV）新品种Juabeng株敏感。CSSD症状表达的严重程度变化很大。5个杂交品种（CRG9006 × CC11、MAN15-2 × CC11、CRG9006 × PLAYA-ALTA2、PA7 × ICS1、PA150 × VENC4-4）耐CSSD。大多数研究性状受加性和非加性基因作用的制约，因此，循环选择和互惠循环选择都是有效的改良育种策略。

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引用次数: 0

Corrigendum to “Quantitative trait loci (QTLs) mapping of seed coat colors in sesame (Sesamum indicum L.): A review” [Ecologic. Genet. Genom. 36 (2025) 100384] 芝麻（Sesamum indicum L.）种皮颜色的数量性状位点（qtl）定位综述[生态学]。麝猫。基因组。36 (2025)100384]

Q3 Agricultural and Biological Sciences

Ecological Genetics and Genomics

Pub Date : 2025-09-01 Epub Date: 2025-07-19 DOI: 10.1016/j.egg.2025.100389

Diriba Shanko , Mebeaselassie Andargie

引用次数: 0

Genotype responsiveness to exogenous auxin in the stem cutting of Coleus scutellarioides 黄芩茎切对外源生长素的基因型响应

Q3 Agricultural and Biological Sciences

Ecological Genetics and Genomics

Pub Date : 2025-09-01 Epub Date: 2025-07-16 DOI: 10.1016/j.egg.2025.100387

Rahmat Budiarto , Syariful Mubarok , Raja Fatah Akbar , Moch Rosyadi Adnan , Supatida Abdullakasim

The commercial ornamental coleus (Coleus scutellarioides) is propagated through stem cuttings to maintain its colorful foliage. This study aimed to evaluate the use of exogenous auxin to enhance the growth of coleus seedlings from stem cuttings. The experiment used a split-plot design to determine the optimal auxin concentration for three commercial coleus genotypes (cv. Pink Splash, Krisna, and Kemuning). The concentrations tested were control, 5 ppm, 10 ppm, 20 ppm, and 40 ppm indole acetic acid (IAA). This study showed a 100 % survival rate in cutting after five weeks, as all cuttings successfully rooted, regardless of genotype and auxin. The most auxin-responsive genotype is cv. Kemuning, showing stable growth improvements across IAA concentrations and greater tolerance to high IAA levels. Pink Splash coleus showed strong growth at moderate auxin levels but experienced a sharp decline at higher concentrations, indicating sensitivity to auxin overdose. In opposite, Krisna coleus exhibited a limited and inconsistent response to auxin. Regardless of genotype, exogenous auxin administration at 10 and 20 ppm significantly enhanced leaf and flush growth, whereas 40 ppm suppressed it. This finding recommends the use of 10 ppm IAA to optimize coleus flush growth during stem cutting propagation.

商业观赏色堇（coleus scutellarioides）是通过茎扦插繁殖以保持其丰富多彩的叶子。本研究旨在探讨外源生长素对花菜扦插苗生长的促进作用。本实验采用裂图设计，确定了三种商业色堇基因型(cv。Pink Splash、Krisna和Kemuning)。试验浓度为对照、5 ppm、10 ppm、20 ppm和40 ppm的吲哚乙酸（IAA）。本研究表明，无论基因型和生长素如何，5周后扦插成活率为100%，所有扦插均成功生根。生长素反应最明显的基因型是cv。在不同的IAA浓度下，克穆宁表现出稳定的生长改善，对高IAA水平的耐受性更强。在中等生长素水平下，粉红水花表现出强劲的生长，但在较高浓度下急剧下降，表明对生长素过量敏感。相反，花楸对生长素的反应有限且不一致。无论基因型如何，外源生长素在10和20 ppm浓度下显著促进叶片和红叶生长，而在40 ppm浓度下则抑制其生长。这一发现建议使用10 ppm的IAA来优化茎切繁殖期间的花柱生长。

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引用次数: 0

Graphic analysis of genetic variation in morphological traits of garden cress (Lepidium sativum L.) 园菜（Lepidium sativum L.）形态性状遗传变异的图解分析

Q3 Agricultural and Biological Sciences

Ecological Genetics and Genomics

Pub Date : 2025-09-01 Epub Date: 2025-05-24 DOI: 10.1016/j.egg.2025.100368

Mehdi Mohebodini , Vahid Rahimi , Naser Sabaghnia , Mohsen Janmohammadi

This study aimed to evaluate a range of morphological traits in 64 garden cress accessions, across Ardabil and Eyvanki, Iran. The experiment was conducted using a randomized block design with three replications. To facilitate the interpretation of the data, graphical analysis was performed using the genotype-by-trait (G × T) biplot model, which explained 73 % and 76 % of the variance in Ardabil and Eyvanki, respectively. The model employed symmetrical scaling of the first two components for both genotypes and traits. The analysis revealed a positive association between seed yield and the number of seeds in the main stem pods at both locations. Additionally, strong positive correlations were observed among key yield components, including lateral branches, total pods per plant, and seeds in the lateral branch pods. Genotypic performance varied across the two environments: in Ardabil, genotype 49 (LEP-108), followed by genotype 47 (LEP-106), exhibited superior performance for most traits. In Eyvanki, genotype 10, followed by genotypes 43 (LEP-76), 48 (LEP-107), and 62 (Tabriz), performed the best across nearly all measured traits. For increasing seed yield, genotype 10 (LEP-22 from Germany) and genotype 49 (LEP-108 from Armenia) were identified as the most promising candidates, so they will enter in multi environmental trails for exploring specific positive genotype by environment interaction and probably cultivar release process. Genotype 52 showed high biomass and moderate seed yield in both locations and may be a suitable candidate for cultivar release. However, all of the studied genotypes will conserve for using in next breeding programs aiming various targets.

本研究旨在评价伊朗Ardabil和Eyvanki地区64份园衣材料的一系列形态性状。试验采用随机区组设计，设3个重复。为了便于对数据进行解释，使用基因型-性状（G × T）双图模型进行了图形分析，该模型分别解释了Ardabil和Eyvanki中73%和76%的方差。该模型对基因型和性状均采用前两个分量的对称缩放。结果表明，两个地点的种子产量与主茎荚内的种子数呈正相关。此外，侧枝、单株总荚果数和侧枝荚果数等关键产量成分之间存在显著正相关。基因型表现在两种环境中存在差异：在Ardabil中，基因型49 （LEP-108）和基因型47 （LEP-106）在大多数性状上表现优异。在Eyvanki品种中，基因型10、基因型43 （LEP-76）、基因型48 （LEP-107）和基因型62 （Tabriz）在几乎所有测定性状中表现最好。为了提高种子产量，基因型10（来自德国的LEP-22）和基因型49（来自亚美尼亚的LEP-108）被认为是最有希望的候选品种，因此它们将通过环境相互作用和可能的品种释放过程进入多环境试验，以探索特定的阳性基因型。基因型52在两个地点均表现出较高的生物量和中等的种子产量，可能是品种释放的合适候选者。然而，所有研究的基因型将被保存下来，用于针对不同目标的下一个育种计划。

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引用次数: 0

De novo plastome assembly of Cymbopogon bhutanicus Noltie, an endemic lemon grass from Bhutan, with geospatial, comparative genomic, and phylogenetic insights 不丹特有柠檬草Cymbopogon bhutanicus Noltie的重新质体组装，具有地理空间，比较基因组学和系统发育见解

Q3 Agricultural and Biological Sciences

Ecological Genetics and Genomics

Pub Date : 2025-09-01 Epub Date: 2025-05-26 DOI: 10.1016/j.egg.2025.100372

Mohan Singh Rana , Nicolas Dierckxsens , Pritesh Bhatt , Bimal K. Chetri

Cymbopogon bhutanicus Noltie, an endemic Bhutanese Poaceae, is valued for its essential oils. While morphological taxonomy offers preliminary insights, molecular data enhances species delimitation. We performed de novo chloroplast genome assembly and used remote sensing to assess environmental influences on its distribution. Geospatial analysis of C. bhutanicus habitat (27.25952°N, 91.40823°E) within a 2km buffer zone utilized Sentinel-2 imagery (2018-2023). Mean Normalized Difference Vegetation Index (NDVI), Enhanced Vegetation Index (EVI), and Soil-Adjusted Vegetation Index (SAVI) values were 0.5974, 0.3356, and 0.3300, respectively. These values indicate robust vegetation health, while observed variations (e.g., NDVI standard deviation of 0.1780) highlight localized microhabitat heterogeneity influenced by topography and moisture gradients. Field observations aligned, confirming its preference for open, well-drained, sun-exposed slopes with minimal canopy. The assembled chloroplast genome (139,701 bp; 38.46 % GC) comprises an 81,663 bp LSC, 12,510 bp SSC, and two 22,764 bp IR regions. Annotation identified 129 genes (87 protein-coding, 34 tRNA, eight rRNA). Phylogenetic analysis confirmed a strong relationship with C. citratus and C. flexuosus (100 % bootstrap). This study enhances C. bhutanicus molecular identification, phylogeny, and ecological understanding, aiding conservation and sustainable use. This study enhances molecular identification, phylogenetic placement, and ecological understanding of C. bhutanicus, contributing to conservation efforts and sustainable utilization.

Cymbopogon bhutanicus Noltie是一种不丹特有的禾科植物，因其精油而受到重视。虽然形态分类学提供了初步的见解，但分子数据增强了物种划分。我们进行了从头叶绿体基因组组装，并利用遥感技术评估了环境对其分布的影响。2018-2023年利用Sentinel-2遥感影像对2km缓冲带内（27.25952°N, 91.40823°E）不丹冷杉生境进行地理空间分析。归一化植被指数（NDVI）均值为0.5974，增强植被指数（EVI）均值为0.3356，土壤调整植被指数（SAVI）均值为0.3300。这些值表明植被健康状况良好，而观测到的变化（如NDVI标准差为0.1780）突出了受地形和湿度梯度影响的局部微生境异质性。实地观察结果一致，证实了它对开放、排水良好、阳光照射的斜坡的偏好，并且树冠最小。组装好的叶绿体基因组(139,701 bp；38.46% GC)包括81,663 bp的LSC， 12,510 bp的SSC和两个22,764 bp的IR区域。注释鉴定出129个基因（87个蛋白编码，34个tRNA， 8个rRNA）。系统发育分析证实了该菌株与柑橘和弯曲柑橘的亲缘关系（100% bootstrap）。本研究提高了对不丹冬青的分子鉴定、系统发育和生态学的认识，有助于不丹冬青的保护和可持续利用。本研究将有助于不丹冬青的分子鉴定、系统发育定位和生态学认识，为不丹冬青的保护和可持续利用做出贡献。

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