Ecological Genetics and Genomics最新文献_第8页

Characterization of metagenomic diversity of soil sediment from Tattapani hot spring located in Chhattisgarh (India) and application of thermophilic bacteria for agrowaste decomposition 印度恰蒂斯加尔邦塔塔帕尼温泉土壤沉积物宏基因组多样性特征及嗜热细菌在农业废弃物分解中的应用

Q3 Agricultural and Biological Sciences

Ecological Genetics and Genomics

Pub Date : 2025-09-01 Epub Date: 2025-07-03 DOI: 10.1016/j.egg.2025.100383

Balram Sahu , Deeppal , Kishan Kumar Raj , Vinay Kumar , Tapas Chowdhury , Ravindra Soni

The current investigation emphasizes on the characterization of bacterial diversity of the Tatapani hot spring in the Balrampur district of Chhattisgarh, India. The isolation, characterization thermophilic bacteria for waste decomposition. Using the clustered OTUs, a total of 56,142 OTUs were identified, of which 81 % were classified under the kingdom Bacteria, while 19 % were assigned to unknown species at the phylum level. Of these, 36 % of OTUs were classified as Proteobacteria and 13 % as Firmicutes. Among the genera revealed from metagenomic analysis, the most abundant was Azospirillum. Simultaneously, culture dependent study revealed several extracellular enzyme producer bacterial isolates present in the samples collected from Tatapani hot spring. Subsequently, on the basis screening results, two bacterial isolates, AMB-T-5W and AMB-T-6S, were selected for 16S rRNA gene sequencing and have similarity with genus Bacillus flexus. These isolated strains exhibited significant extracellular enzyme activities, including amylase, cellulase, lipase, protease, and xylanase. Remarkably, the isolates demonstrated the ability to produce these enzymes. The isolates were tested for the biodegradation of rice, wheat, and corn straw as decomposing agents, and growth parameters were analyzed under spinach biomass return, with and without the inclusion of the decomposition agent at varying time intervals. The findings suggest that these thermophilic bacteria and their enzymes hold considerable potential for waste decomposition and industrial applications.

目前的研究重点是印度恰蒂斯加尔邦Balrampur地区Tatapani温泉的细菌多样性特征。垃圾分解嗜热细菌的分离、表征。利用聚类otu，共鉴定出56142个otu，其中81%归属于细菌界，19%归属于门水平的未知物种。其中，36%的otu被归类为变形菌门，13%被归类为厚壁菌门。在宏基因组分析中发现的属中，数量最多的属是偶氮螺旋藻。同时，培养依赖性研究发现，从塔塔帕尼温泉采集的样品中分离出了几种胞外酶产生菌。随后，在筛选结果的基础上，筛选出与弯曲芽孢杆菌属具有相似性的AMB-T-5W和AMB-T-6S两株分离菌进行16S rRNA基因测序。这些分离菌株表现出显著的胞外酶活性，包括淀粉酶、纤维素酶、脂肪酶、蛋白酶和木聚糖酶。值得注意的是，分离物显示出产生这些酶的能力。以水稻、小麦和玉米秸秆为分解剂对分离菌株进行了生物降解试验，并在不同时间间隔添加和不添加分解剂的情况下，对菠菜生物量归还条件下的生长参数进行了分析。研究结果表明，这些嗜热细菌及其酶在废物分解和工业应用方面具有相当大的潜力。

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引用次数: 0

Molecular barcoding of yellow-lipped sea krait, Laticauda colubrina (Schneider, 1799) from the Andaman Islands, India 印度安达曼群岛黄唇海蛇，Laticauda colubrina （Schneider, 1799）的分子条形码

Q3 Agricultural and Biological Sciences

Ecological Genetics and Genomics

Pub Date : 2025-09-01 Epub Date: 2025-06-27 DOI: 10.1016/j.egg.2025.100381

Jessica Barman , Samson Rokkarukala , Anwesh Maile , S. Rajendra , R. Mohanraju

The present study details the taxonomic identification and DNA barcoding of a sea krait species, Laticauda colubrina (Schneider, 1799), from the Andaman Islands, India. Sea kraits, belonging to the genus Laticauda, are semiaquatic and venomous elapid marine reptiles found in coastal environments. Even though the Laticauda population are abundant in Andaman and Nicobar Archipelago, molecular studies have not yet been undertaken on the Andaman and Nicobar Islands as well as Indian waters. In the current study, the species was identified through morphological taxonomic characters, and mitochondrial cytochrome c oxidase I (COI) gene analysis was followed to confirm the sea krait species (GenBank ID: OQ888166.1) which resulted in a 99 % coverage and 97.44 % identity with mitochondrial cytochrome oxidase subunit 1 (COI) gene of Laticauda colubrina voucher USNM: Herp:577712 (GenBank ID: MH274247.1).

本文对印度安达曼群岛的一种海蛇Laticauda colubrina （Schneider, 1799）进行了分类鉴定和DNA条形码分析。海鳞蟹属，是一种半水生、有毒的海生爬行动物，生活在沿海环境中。尽管拉蒂库达种群在安达曼和尼科巴群岛丰富，但尚未对安达曼和尼科巴群岛以及印度水域进行分子研究。本研究通过形态分类特征对该物种进行鉴定，并对其进行线粒体细胞色素c氧化酶I （COI）基因分析，对其进行鉴定（GenBank ID: OQ888166.1），结果表明其与Laticauda colubrina voucher USNM: Herp:577712 （GenBank ID: MH274247.1）的线粒体细胞色素氧化酶亚基1 （COI）基因的同源性为97.44%，覆盖率为99%。

引用次数: 0

From wild to domesticated: exploring selection signatures caused by domestication in Carica papaya 从野生到驯化：探索番木瓜驯化引起的选择特征

Q3 Agricultural and Biological Sciences

Ecological Genetics and Genomics

Pub Date : 2025-09-01 Epub Date: 2025-05-23 DOI: 10.1016/j.egg.2025.100367

Mónica I. Jiménez-Rojas , Pedro J. Ruiz-Gil , Mauricio Heredia-Pech , Mariana Chávez-Pesqueira

During plant domestication, artificial selection acts on traits to meet human needs, interacting with other evolutionary forces and shaping genetic diversity across the genome. However, its impact on perennial fruit crops like Carica papaya remains poorly understood. In this study, we investigated genomic regions under selection during papaya domestication using 2710 single nucleotide polymorphisms (SNPs) across 350 accessions—229 wild and 121 domesticated. Genetic structure was assessed through hierarchical clustering and three statistical methods (FST-based Fdist2, BayeScan, and XP-CLR) were applied to detect selection signatures. The results revealed two clearly differentiated genetic groups corresponding to wild and domesticated papaya. Eighteen loci showed strong evidence of selection, with six containing annotated genes linked to traits influenced by domestication. These genes were primarily associated with fruit traits, plant development, and fruit development. The findings align with expected patterns of selection under domestication, where traits beneficial for cultivation and yield are preferentially retained. This study not only advances the understanding of how domestication shapes the papaya genome but also identifies candidate genes that can serve as valuable targets for breeding programs. Moreover, the genetic differentiation observed reinforces the importance of conserving wild papaya populations as reservoirs of genetic diversity for future crop improvement.

在植物驯化过程中，人工选择作用于性状以满足人类的需要，与其他进化力量相互作用并形成整个基因组的遗传多样性。然而，它对番木瓜等多年生水果作物的影响仍然知之甚少。在这项研究中，我们利用350个品种（229个野生品种和121个驯化品种）的2710个单核苷酸多态性（SNPs）研究了番木瓜驯化过程中选择的基因组区域。通过分层聚类评估遗传结构，并采用三种统计方法（基于fst的Fdist2、BayeScan和XP-CLR）检测选择特征。结果表明，野生木瓜和驯化木瓜有明显的遗传群差异。18个位点显示出强烈的选择证据，其中6个位点包含与驯化影响的性状相关的注释基因。这些基因主要与果实性状、植物发育和果实发育有关。这一发现与驯化下的预期选择模式相一致，即有利于栽培和产量的性状被优先保留。这项研究不仅促进了对驯化如何塑造木瓜基因组的理解，而且还确定了可以作为育种计划有价值目标的候选基因。此外，观察到的遗传分化强化了保护野生木瓜群体作为遗传多样性储存库对未来作物改良的重要性。

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引用次数: 0

Molecular phylogeny of genus Nothopegia Blume nom.cons. (Anacardiaceae) in Central Western Ghats Karnataka, based on the ITS, rbcL and trnLF markers 褐藻属的分子系统发育。基于ITS， rbcL和trnLF标记，在卡纳塔克邦中西部高塞山脉（Ghats Karnataka）中发现（Anacardiaceae）

Q3 Agricultural and Biological Sciences

Ecological Genetics and Genomics

Pub Date : 2025-09-01 Epub Date: 2025-06-21 DOI: 10.1016/j.egg.2025.100379

Venkatesh Jugal , H.C. Shrishail , P Shivakumar Singh , Kamal Shah , Nagendra Singh Chauhan

Nothopegia Blume nom.cons genus is an Anacardiaceae family, a small dioecious tree and shrub, Four species can be located within the Central Western Ghats in Karnataka. These species are difficult to differentiate based solely on morphological traits because of their almost identical characteristics and endemic position in the area. Using a variety of techniques, individuals attempt to understand the genus' taxonomic difficulty. The current research used DNA information to explain the genus systematic evolutionary phylogeny. Also, we have to use certain primers for molecular phylogeny characterization, Four Nothopegia species sequence result, twenty one ingroup taxa and two outgroup species query sequences were retrieved from NCBI. For molecular studies purposes Freshly obtained leaf samples were used to isolate whole genomic DNA by the CTAB method, This DNA was then amplified and sequenced using several primer combinations like sequences of ITS, rbcL and trnLF, to compare the phylogenetic results the sequence of each taxon was first Aligned by Mega version11.0.10 and create consensus sequences by using Sequencher v5.2 software. The aligned sequences will undergo BLAST analysis at the NCBI. Subsequently, the consensus sequences will be submitted to the NCBI. Maximum parsimony analysis was carried out by PAUP v 4.0a169. The combining sequence of ITS, rbcL, and trnLF primers, Analysis of Maximum likelihood in raxmlGUI version 2.0, and Bayesian analysis was drawn using MrBayes v3.2.7a software, edited sequenced It was found that When building a tree of phylogeny, the data proved quite beneficial. A clear phylogeny of the genus may be constructed by adding different species from different regions.

nothoopegia Blume nomo .cons属是一种小的雌雄异株乔木和灌木，分布在卡纳塔克邦的中西部高止山脉。由于这些物种几乎相同的特征和在该地区的特有位置，因此很难仅根据形态特征进行区分。使用各种技术，个体试图理解属的分类困难。目前的研究利用DNA信息来解释属的系统进化系统。在NCBI中检索到4个nothoopegia物种序列结果、21个群内分类群和2个群外物种查询序列。在分子研究方面，利用新鲜获得的叶片样品，用CTAB法分离出全基因组DNA，然后用ITS、rbcL和trnLF等引物组合进行扩增和测序，比较各分类单元的系统发育结果，首先用Mega version11.0.10进行序列比对，然后用Sequencher v5.2软件建立一致序列。比对后的序列将在NCBI进行BLAST分析。随后，将一致序列提交给NCBI。最大简约性分析采用PAUP v 4.0a169进行。利用MrBayes v3.2.7a软件绘制ITS、rbcL和trnLF引物的组合序列，在raxmlGUI version 2.0中进行最大似然分析，并进行贝叶斯分析，编辑测序后发现，在构建系统发育树时，数据是非常有益的。通过添加来自不同地区的不同物种，可以构建出该属的清晰系统发育。

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引用次数: 0

Butternut pumpkin-powdery mildew disease interaction as influenced by sowing type and date 播型和播期对冬南瓜白粉病互作的影响

Q3 Agricultural and Biological Sciences

Ecological Genetics and Genomics

Pub Date : 2025-09-01 Epub Date: 2025-05-27 DOI: 10.1016/j.egg.2025.100375

Hilma Sabet , Hossein Ali Asadi-Gharneh , Mehdi Nasr-Esfahani

Butternut pumpkin (Cucurbita pepo L.) is an economically important vegetable and summer crop. The seeds have high nutritional value due to their edible oil and protein content with a high medicinal value. Butternut pumpkin is attacked by several fungal diseases one of which is powdery mildew (PM) caused by Podosphaera xanthii. In this study, we analyzed the effect of sowing type and date on 14 local butternut pumpkin genotypes for resistance against powdery mildew. The two different sowing types were greenhouse vs. open field and the two sowing dates were first week of May vs. 3rd week of May 2021–22. The results showed that powdery mildew severity percentage (PMSP) was 42 % in the field #1 sown in the first week of May compared to the field #2 with 48 % severity sown two weeks later than the field #1 indicating 6 % increase in PMSP significantly. On the other hand, PMSP severity in the greenhouse was 55.2 % which was 8–13 % greater than those occurring in the field. There was also a wide variation in disease severity among the genotypes. The highest PMSP was recorded in” Orumiyeh1” (84.8 %) followed by “Maragheh3” (82 %), and the lowest ones were in “Maragheh1” (9.4 %) and “Shahreza” (6.1 %) genotypes. In conclusion, we found that the ideal conditions for cultivating butternut pumpkin with the least PMSP include sowing during the first week of May in field #1 and the use of resistant cultivars such as “Maragheh1” and “Shahreza” as a part of an integrated pest management program.

冬南瓜（Cucurbita pepo L.）是一种重要的经济蔬菜和夏季作物。由于其食用油和蛋白质含量高，具有很高的药用价值，具有很高的营养价值。冬南瓜受到几种真菌病害的侵袭，其中一种病害是由黄足霉引起的白粉病。本研究分析了播型和播期对14个地方冬南瓜基因型抗白粉病的影响。两种播种类型分别为温室和露天，播种日期分别为2021 - 2022年5月第一周和5月第3周。结果表明，5月第一周播种的1号田比2号田的白粉病严重程度百分比（PMSP）为42%，比1号田晚两周播种的白粉病严重程度百分比为48%，表明PMSP显著增加了6%。温室PMSP严重程度为55.2%，比田间高8 ~ 13个百分点。基因型之间的疾病严重程度也有很大差异。PMSP最高的是“Orumiyeh1”（84.8%），其次是“Maragheh3”（82%），最低的是“Maragheh1”（9.4%）和“Shahreza”（6.1%）。综上所述，我们发现栽培PMSP最低的冬南瓜的理想条件是在5月的第一周播种在1号田，并使用抗病品种如“Maragheh1”和“Shahreza”作为害虫综合治理计划的一部分。

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引用次数: 0

Applying trnL-trnF and ITS loci for species identification of agarwood: A study from Assam, India 应用trnL-trnF和ITS位点鉴定沉香属植物：印度阿萨姆邦的研究

Q3 Agricultural and Biological Sciences

Ecological Genetics and Genomics

Pub Date : 2025-09-01 Epub Date: 2025-07-24 DOI: 10.1016/j.egg.2025.100391

Barasha Sarma, Ankur Das, Sofia Banu

Aquilaria species are renowned for producing agarwood, a dark, fragrant resin with significant medicinal and economic value. Due to high market demand, overexploitation, and limited natural availability, the prevalence of counterfeit agarwood products has increased, highlighting the need for accurate species identification to ensure product authenticity and support conservation efforts. Traditional morphological methods are often inadequate for reliable species-level discrimination, particularly in semi-processed or traded materials. In this study, DNA was extracted from 20 commercial Aquilaria wood log samples, and two genomic loci, viz., trnL-trnF (chloroplast) and ITS (nuclear), were PCR-amplified and analysed. Sequence identity was assessed via BLASTn, and phylogenetic relationships were reconstructed using MEGA X. While the ITS region showed limited taxonomic resolution due to high sequence similarity among A. agallochum, A. yunnanensis, and A. malaccensis, the trnL-trnF locus consistently identified most samples as A. agallochum, demonstrating superior species-level resolving power. However, ambiguous cases suggest that single-locus markers may not always be conclusive. These findings underscore the value of trnL-trnF as a practical and reliable molecular marker for agarwood authentication, while also pointing potential of full chloroplast genome strategies for more definitive species identification across the Aquilaria genus.

沉香属以生产沉香木而闻名，沉香木是一种具有重要药用和经济价值的深色芳香树脂。由于市场需求高、过度开发和自然资源有限，假冒沉香木产品的流行有所增加，这突出了对准确物种鉴定的需求，以确保产品的真实性并支持保护工作。传统的形态学方法往往不足以进行可靠的物种水平的区分，特别是在半成品或交易材料中。本研究从20份商业沉香原木样品中提取DNA，对trnL-trnF（叶绿体）和ITS（核）两个基因组位点进行pcr扩增和分析。利用BLASTn进行序列一致性评估，并用MEGA x重建系统发育关系。ITS区域由于沉香、云南沉香和malaccensis之间的序列高度相似，其分类分辨率有限，而trnL-trnF位点一致地将大多数样本识别为沉香，显示出优越的物种水平分辨率。然而，模棱两可的病例表明，单位点标记可能并不总是决定性的。这些发现强调了trnL-trnF作为沉香鉴定实用可靠的分子标记的价值，同时也指出了全叶绿体基因组策略在沉香属更明确的物种鉴定中的潜力。

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引用次数: 0

Functional metagenomics: A potential tool for mining of biomolecules from environmental samples 功能宏基因组学：从环境样品中挖掘生物分子的潜在工具

Q3 Agricultural and Biological Sciences

Ecological Genetics and Genomics

Pub Date : 2025-09-01 Epub Date: 2025-05-23 DOI: 10.1016/j.egg.2025.100369

Vivek Kumar , Anjali Singh , Vijay Shankar Singh , Rajan Chaurasia , Mahesh Rao , Pramod Kumar Sahu , Durgesh Kumar Jaiswal

Functional metagenomics is emerging as a transformative tool for discovering biomolecules from environmental samples, offering unprecedented insights into microbial diversity and functionality. This review explores the potential of functional metagenomics to uncover novel enzymes, antibiotics, and therapeutic compounds by analyzing environmental DNA (eDNA) without the constraints of traditional culture-based methods. The vast microbial biomass in diverse ecosystems, from soil to extreme habitats, harbors a wealth of genetic resources that can be harnessed for biotechnological applications. Researchers can use modern sequencing techniques and functional screening approaches to find and characterize genes with advantageous features, such as bioremediation capacities and industrially relevant enzymes. Despite challenges in DNA extraction and host expression systems, integrating functional metagenomics with multi-omics approaches promises to enhance our understanding of microbial interactions and facilitate the development of eco-friendly bioprocesses. This review underscores the importance of functional metagenomics in expanding our repertoire of biomolecules and highlights its potential to significantly impact biotechnology and environmental sustainability.

功能宏基因组学正在成为从环境样本中发现生物分子的变革性工具，为微生物多样性和功能提供了前所未有的见解。这篇综述探讨了功能宏基因组学的潜力，通过分析环境DNA （eDNA）发现新的酶，抗生素和治疗化合物，而不受传统的基于培养的方法的限制。从土壤到极端生境的各种生态系统中，蕴藏着丰富的遗传资源，可用于生物技术应用。研究人员可以使用现代测序技术和功能筛选方法来发现和表征具有优势特征的基因，例如生物修复能力和工业相关的酶。尽管在DNA提取和宿主表达系统方面存在挑战，但将功能宏基因组学与多组学方法相结合有望增强我们对微生物相互作用的理解，并促进生态友好生物过程的发展。这篇综述强调了功能宏基因组学在扩大我们的生物分子库方面的重要性，并强调了它对生物技术和环境可持续性的重大影响。

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引用次数: 0

Ecological and genomic perspectives on fungal plastic biodegradation: Research progress, opportunities and challenges 真菌塑料生物降解的生态学和基因组学观点：研究进展、机遇和挑战

Q3 Agricultural and Biological Sciences

Ecological Genetics and Genomics

Pub Date : 2025-09-01 Epub Date: 2025-06-11 DOI: 10.1016/j.egg.2025.100378

Aubrey Dickson Chigwada, Memory Tekere, Henry Joseph Oduor Ogola

Plastic pollution has emerged as a pervasive global threat to ecosystems, with rising production and ineffective waste management accelerating environmental accumulation. As sustainable bioremediation strategies become increasingly vital, fungi have gained attention for their capacity to degrade recalcitrant plastics in natural and anthropogenically impacted environments. Fungal taxa isolated from diverse ecological niches, such as landfills, marine sediments, insect guts, mulch films, and herbivore dung, have can colonize plastic surfaces, form biofilms, and enzymatically depolymerize synthetic polymers. Key enzymes include hydrolases (cutinases, esterases, and lipases) and oxidoreductases (laccases, peroxidases, and peroxygenases). These enzymes cleave plastic polymers into smaller units (monomers), which are further mineralized into CO₂ and CH₄. Recent advances in ecological genomics and systems biology have revealed the genetic architectures, regulatory pathways, and enzyme diversity underpinning fungal plastic degradation. Omics-driven investigations, such as metagenomics, transcriptomics, and comparative genomics, have identified lineage-specific adaptations and functional genes involved in plastic catabolism. Despite this progress, fungal degradation still faces constraints. These include slow enzyme kinetics, incomplete metabolic conversion, and challenges in scaling up bioprocesses. This review integrates ecological and genomic insights into fungal plastic biodegradation, highlighting species diversity, enzymatic mechanisms, and functional pathways. It also identifies research gaps, such as the need for optimization, engineered microbial consortia, and pilot-scale and real-world environmental applications of fungal plastic degradation systems. Finally, the review discusses emerging innovations, including gene editing, enzyme stabilization, and scalable fungal bioreactors, as promising directions for translating fungal plastic degradation into real-world environmental solutions.

塑料污染已成为全球生态系统普遍面临的威胁，不断增长的产量和无效的废物管理加速了环境积累。随着可持续的生物修复策略变得越来越重要，真菌因其在自然和人为影响环境中降解难降解塑料的能力而受到关注。从不同的生态位中分离出来的真菌分类群，如垃圾填埋场、海洋沉积物、昆虫肠道、地膜和食草动物粪便，可以在塑料表面定植，形成生物膜，并通过酶解聚合合成聚合物。关键酶包括水解酶（角质酶、酯酶和脂肪酶）和氧化还原酶（漆酶、过氧化物酶和过氧酶）。这些酶将塑料聚合物分解成更小的单元（单体），这些单体进一步矿化成二氧化碳和甲烷。生态基因组学和系统生物学的最新进展揭示了真菌塑料降解的遗传结构、调控途径和酶多样性。基因组学驱动的研究，如宏基因组学、转录组学和比较基因组学，已经确定了与塑料分解代谢有关的谱系特异性适应和功能基因。尽管取得了这些进展，但真菌降解仍然面临限制。这些包括缓慢的酶动力学，不完全的代谢转化，以及扩大生物过程的挑战。本文综述了真菌塑料生物降解的生态学和基因组学见解，突出了物种多样性，酶机制和功能途径。它还确定了研究空白，例如对优化的需求，工程微生物联盟，真菌塑料降解系统的中试规模和实际环境应用。最后，综述讨论了新兴的创新，包括基因编辑，酶稳定和可扩展的真菌生物反应器，作为将真菌塑料降解转化为现实环境解决方案的有希望的方向。

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引用次数: 0

GGE-biplot investigation of climate-smart Senna (Cassia angustifolia Vahl.) genotypes' agronomic performance, biomass, and yield stability across years/environments 气候智能型塞纳（Cassia angustifolia Vahl.）基因型农艺性能、生物量和产量稳定性的ge -双标图研究

Q3 Agricultural and Biological Sciences

Ecological Genetics and Genomics

Pub Date : 2025-09-01 Epub Date: 2025-07-25 DOI: 10.1016/j.egg.2025.100390

Ashish Kumar , R.K. Lal , Ramesh Kumar Srivastava , S. Venugopal , A.C. Jnanesha , A. Niranjana Kumar , Anil Kumar Gupta

Senna is a popular medicinal herb used in almost all healthcare systems as a laxative. The primary issue this research addresses is the significant yield variability in Senna (Cassia angustifolia) across different environments, which hinders the identification and adoption of genotypes with consistent performance. This instability arises from strong genotype × environment interactions, making it difficult for breeders to select robust, high-yielding lines suitable for diverse agro-ecological zones. To overcome this challenge, our study employed AMMI (Additive Main Effects and Multiplicative Interaction) and GGE biplot analyses across multi-environment trials to assess yield performance, adaptability, and stability of various genotypes. These advanced statistical tools allowed us to separate main effects from interactions and visually interpret genotype responses across locations. The biplot depicts the which-won-where trend in various situations, illustrating the adaptability of the lines. PC1 and PC2's x and y axes explained 80.30 % and 9.3 % of the total variation (89.60 %), and 61.0 % and 21.30 % (82.30 %). The six environments were categorized, and then appropriate genotypes were chosen for each. Our findings identified genotypes SEN-1, 10, and 12 as the most stable and high-yielding across the tested environments. These genotypes demonstrated minimal interaction effects and superior adaptability, indicating their potential for widespread cultivation and inclusion in future breeding programs to improve Senna production. Therefore, this research not only fills a critical gap in the stability analysis of Senna but also provides concrete recommendations for genotype deployment in varied Indian agro-climates for large-scale production.

番泻草是一种流行的草药，几乎在所有的医疗保健系统中用作泻药。本研究解决的主要问题是塞纳（决明子）在不同环境下的显著产量变异，这阻碍了具有一致性能的基因型的鉴定和采用。这种不稳定性源于基因型与环境的强烈相互作用，使得育种者难以选择适合不同农业生态区的健壮高产品系。为了克服这一挑战，我们的研究采用了AMMI （Additive Main Effects and multiplative Interaction）和GGE双图分析，在多环境试验中评估了不同基因型的产量性能、适应性和稳定性。这些先进的统计工具使我们能够从相互作用中分离出主要影响，并直观地解释不同地点的基因型反应。双标图描绘了在各种情况下哪个赢了哪里的趋势，说明了线的适应性。PC1和PC2的x轴和y轴分别解释了总变异的80.30%和9.3%(89.60%)，61.0%和21.30%（82.30%）。对6种环境进行分类，选择相应的基因型。我们的研究结果确定SEN-1、10和12基因型在测试环境中是最稳定和高产的。这些基因型表现出最小的相互作用效应和优越的适应性，表明它们具有广泛种植和纳入未来育种计划以提高番泻草产量的潜力。因此，本研究不仅填补了番泻稳定性分析的关键空白，而且为在印度不同农业气候条件下进行基因型配置以实现大规模生产提供了具体建议。

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引用次数: 0

Microbiome of different gut compartments of banana pseudostem weevil Odoiporus longicollis (Coleoptera: Curculionidae) offers an understanding of site-specific diversity and metabolism: Whole-metagenome shotgun sequencing approach 香蕉假茎象Odoiporus longicollis不同肠道区室的微生物组（鞘翅目：curculionidis科）提供了位点特异性多样性和代谢的理解：全宏基因组鸟枪法测序方法

Q3 Agricultural and Biological Sciences

Ecological Genetics and Genomics

Pub Date : 2025-09-01 Epub Date: 2025-08-23 DOI: 10.1016/j.egg.2025.100397

Sreeramulu Bhuvaragavan , Kannan Sruthi , Akshaya Panigrahi , Sundaram Janarthanan

The pseudostem weevil Odoiporus longicollis, a major pest of banana confers serious crop losses. The cellulose- and terpene-rich diet requires a high reliance on gut microbiome for nutrition. While Puranik et al. (2024) explored the whole gut bacteriome (16S) of this pest, spatial distribution of microbiome across gut sections remained uncharacterized. Here we present the region-specific microbiome of foregut, midgut and hindgut of O. longicollis, encompassing bacteria, fungi and other eukaryotes. Among bacteria, Firmicutes (Bacilli) predominated in the foregut while Proteobacteria (Gammaproteobacteria) dominated midgut and hindgut. Erysipelotrichia was notably higher in the hindgut. The Enterobacteriaceae (Klebsiella, Enterobacter) subjugated all sections with differential distribution of Raoultella, Citrobacter and Escherichia. Lactobacillaceae (Leuconostoc, Lactiplantibacillus) were prominent in foregut while Streptococcaceae (Lactococcus) dominated the mid- and hindgut. Notably, the primary endosymbiont Candidatus Nardonella was restricted to the foregut. Fungal phyla Ascomycota and Basidiomycota were abundant. Debaryomycetaceae (Scheffersomyces, Millerozyma, Candida) dominated the foregut and hindgut, whereas Saccharomycodaceae (Hanseniaspora, Yarrowia) characterized the midgut. Other eukaryotes i.e., Alveolata, Apicomplexa, Oomycota, Discoba, Amoebozoa, Chloroplast-Stramenopile (CS) clade, Euglenozoa, Heterolobosea and Perkinsozoa were distributed differentially. The hindgut emerged as the species-rich and taxonomically diverse region. Fungal species were rich in foregut and midgut, and diverse in hindgut and foregut. Gene profiling revealed distinct patterns in carbohydrate metabolism, terpene degradation and nitrogen cycle, uncovering dependent and independent modes of cellulolysis in O. longicollis. The findings improved the understanding of the spatial dynamics and functional potential of the O. longicollis gut microbiome, offering implications for microbiome-based pest control strategies.

假茎象鼻虫是香蕉的主要害虫，造成严重的作物损失。富含纤维素和萜烯的饮食需要高度依赖肠道微生物群提供营养。虽然Puranik等人（2024）探索了这种害虫的整个肠道细菌群（16S），但微生物群在肠道各部分的空间分布仍未表征。在这里，我们介绍了O. longicollis的前肠、中肠和后肠的区域特异性微生物组，包括细菌、真菌和其他真核生物。细菌中，厚壁菌门（Bacilli）在前肠中占优势，而变形菌门（Gammaproteobacteria）在中肠和后肠中占优势。后肠丹毒毛菌明显增多。肠杆菌科（克雷伯氏菌、肠杆菌）在各菌群中占主导地位，Raoultella、Citrobacter和Escherichia分布不同。乳酸菌科（Leuconostoc, Lactiplantibacillus）以前肠为主，链球菌科（Lactococcus）以中、后肠为主。值得注意的是，主要的内共生候选者Nardonella仅限于前肠。真菌门子囊菌门和担子菌门丰富。Debaryomycetaceae （Scheffersomyces, Millerozyma, Candida）主要分布在前肠和后肠，而Saccharomycodaceae （Hanseniaspora, Yarrowia）主要分布在中肠。其他真核生物如肺泡藻、顶复合体、卵菌藻、盘藻藻、阿米巴虫、叶绿体-层桩（CS）分支、裸藻、异藻藻和珀金虫等分布差异较大。后肠是物种丰富和分类多样化的区域。前肠和中肠真菌种类丰富，后肠和前肠真菌种类多样。基因图谱揭示了其碳水化合物代谢、萜烯降解和氮循环的不同模式，揭示了其纤维素分解的依赖和独立模式。研究结果提高了人们对长结肠弧菌肠道微生物群空间动态和功能潜力的认识，为基于微生物群的害虫防治策略提供了参考。

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