Microchemical Journal最新文献_第8页

Recent progress in noble metal-based single-atom nanozymes for biomedical applications 用于生物医学应用的贵金属基单原子纳米酶的最新进展

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2024-09-21 DOI: 10.1016/j.microc.2024.111731

Nanozymes are nanomaterials with enzymatic properties designed to overcome the inherent limitations of natural enzymes. They can mimic various enzyme activities with durability, low cost, and tunable catalytic properties. With the rapid development of nanotechnology, biotechnology, catalytic science, and computational chemistry, remarkable progress has been made in the research of nanozymes. However, compared with natural enzymes, the reported nanozymes possess lower catalytic activity, which limits their further application. Single-atom nanozymes (SAzymes) are a new type of nanozymes that have been widely explored in recent years. With their maximum active site density and well-defined coordination environment, SAzymes have demonstrated excellent catalytic performance, showing broad application prospects in many fields such as biosensing, antibacterial, and tumor therapy. Furthermore, the well-defined atomic coordination and electron configuration of SAzymes provide strong support to reveal the complex mechanism of their catalytic reaction. Particularly, the noble metal-based SAzymes precisely synthesized on the atomic scale, inherit the advantages of SAzymes, while combining the excellent electronic conductivity, high stability and good catalytic activity of noble metals. This effectively reduces the production cost and gives the noble metal-based SAzymes a great potential for practical applications. In this review, we focus on the recent advances of noble metal-based SAzymes in biomedicine and discuss their underlying challenges as well as future prospects.

纳米酶是一种具有酶特性的纳米材料，旨在克服天然酶的固有局限性。它们可以模拟各种酶的活性，具有耐久性、低成本和可调的催化特性。随着纳米技术、生物技术、催化科学和计算化学的快速发展，纳米酶的研究取得了显著进展。然而，与天然酶相比，已报道的纳米酶具有较低的催化活性，这限制了它们的进一步应用。单原子纳米酶（SAzymes）是近年来被广泛探索的一种新型纳米酶。单原子纳米酶具有最大的活性位点密度和明确的配位环境，表现出优异的催化性能，在生物传感、抗菌、肿瘤治疗等多个领域展现出广阔的应用前景。此外，SAzymes 明确的原子配位和电子构型为揭示其催化反应的复杂机理提供了有力支持。特别是在原子尺度上精确合成的贵金属基 SAzymes，在继承 SAzymes 优点的同时，兼具贵金属优异的电子传导性、高稳定性和良好的催化活性。这有效降低了生产成本，使贵金属基 SAzymes 在实际应用中大有可为。在这篇综述中，我们将重点介绍贵金属基 SAzymes 在生物医学领域的最新进展，并讨论其背后的挑战和未来前景。

{"title":"Recent progress in noble metal-based single-atom nanozymes for biomedical applications","authors":"","doi":"10.1016/j.microc.2024.111731","DOIUrl":"10.1016/j.microc.2024.111731","url":null,"abstract":"<div><div>Nanozymes are nanomaterials with enzymatic properties designed to overcome the inherent limitations of natural enzymes. They can mimic various enzyme activities with durability, low cost, and tunable catalytic properties. With the rapid development of nanotechnology, biotechnology, catalytic science, and computational chemistry, remarkable progress has been made in the research of nanozymes. However, compared with natural enzymes, the reported nanozymes possess lower catalytic activity, which limits their further application. Single-atom nanozymes (SAzymes) are a new type of nanozymes that have been widely explored in recent years. With their maximum active site density and well-defined coordination environment, SAzymes have demonstrated excellent catalytic performance, showing broad application prospects in many fields such as biosensing, antibacterial, and tumor therapy. Furthermore, the well-defined atomic coordination and electron configuration of SAzymes provide strong support to reveal the complex mechanism of their catalytic reaction. Particularly, the noble metal-based SAzymes precisely synthesized on the atomic scale, inherit the advantages of SAzymes, while combining the excellent electronic conductivity, high stability and good catalytic activity of noble metals. This effectively reduces the production cost and gives the noble metal-based SAzymes a great potential for practical applications. In this review, we focus on the recent advances of noble metal-based SAzymes in biomedicine and discuss their underlying challenges as well as future prospects.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":null,"pages":null},"PeriodicalIF":4.9,"publicationDate":"2024-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142310628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fluorescence-based determination of tobramycin using a portable smartphone-based device 使用基于智能手机的便携式设备，基于荧光测定妥布霉素

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2024-09-20 DOI: 10.1016/j.microc.2024.111713

The study presents the development of a robust portable smartphone-based device for determining tobramycin in drugs and urine by a fluorescence-based method. As existing analytical methods for the determination of tobramycin often require complex equipment and time-consuming procedures, the proposed approach offers simplicity, portability and real-time monitoring capabilities. The spectrofluorimetric method is based on the reaction of tobramycin and fluorescamine in an alkaline medium. The measurement of analytical signals was enabled with a specially designed device integrating a 3D printed adapter, LEDs, a macro lens, an Arduino-based microcontroller, and a smartphone. The traditional spectrofluorimetry was used as a reference method. Preliminary and optimisation studies were conducted to select the most suitable experimental conditions (e.g. reagent concentration and reaction time) as well as instrumental parameters (e.g. selection of the appropriate RGB model component, ISO, and white balance). The performance of the method was validated, and then verified against conventional spectrofluorimetry, showing comparable accuracy and precision. The method implemented in a smartphone-based device showed a linear range of 0.10–1.00 mg L^-1 with a detection limit of 0.03 mg L^-1. Synthetic samples were tested to verify analytical usability, followed by analysis of pharmaceutical and urine samples. The method showed good precision (CV<3.4 %) and recovery values (92.3–114.1 %), consistent with spectrofluorimetric results (CV<5.9 %, recovery 92.3–119.8 %). Comparison with other methods highlighted the simplicity and efficiency of the developed approach. The research reveals that it is competitive with existing well-established methods. The proposed device offers reliable results with low reagent and sample consumption, making it suitable for on-the-go analysis in non-laboratory conditions.

本研究介绍了一种基于智能手机的强大便携式设备的开发情况，该设备采用基于荧光的方法测定药物和尿液中的妥布霉素。现有的妥布霉素分析方法通常需要复杂的设备和耗时的程序，而本研究提出的方法则具有简便、便携和实时监测的特点。光谱荧光法是基于妥布霉素和荧光胺在碱性介质中的反应。分析信号的测量由一个专门设计的装置实现，该装置集成了 3D 打印适配器、LED、微距镜头、基于 Arduino 的微控制器和智能手机。传统的光谱荧光测定法被用作参考方法。进行了初步和优化研究，以选择最合适的实验条件（如试剂浓度和反应时间）以及仪器参数（如选择合适的 RGB 模型组件、ISO 和白平衡）。对该方法的性能进行了验证，然后与传统的光谱荧光测定法进行了比较，结果表明其准确度和精确度相当。该方法在智能手机设备中的线性范围为 0.10-1.00 mg L-1，检测限为 0.03 mg L-1。对合成样品进行了测试，以验证分析的可用性，随后对药物和尿液样品进行了分析。该方法显示出良好的精密度（CV<3.4 %）和回收率（92.3-114.1 %），与光谱荧光法的结果（CV<5.9 %，回收率 92.3-119.8%）一致。与其他方法的比较凸显了所开发方法的简便性和高效性。研究表明，该方法与现有的成熟方法相比具有竞争力。所提议的装置试剂和样品消耗量低，结果可靠，适合在非实验室条件下进行随用随测的分析。

{"title":"Fluorescence-based determination of tobramycin using a portable smartphone-based device","authors":"","doi":"10.1016/j.microc.2024.111713","DOIUrl":"10.1016/j.microc.2024.111713","url":null,"abstract":"<div><div>The study presents the development of a robust portable smartphone-based device for determining tobramycin in drugs and urine by a fluorescence-based method. As existing analytical methods for the determination of tobramycin often require complex equipment and time-consuming procedures, the proposed approach offers simplicity, portability and real-time monitoring capabilities. The spectrofluorimetric method is based on the reaction of tobramycin and fluorescamine in an alkaline medium. The measurement of analytical signals was enabled with a specially designed device integrating a 3D printed adapter, LEDs, a macro lens, an Arduino-based microcontroller, and asmartphone. The traditional spectrofluorimetry was used as a reference method. Preliminary and optimisation studies were conducted to select the most suitable experimental conditions (e.g. reagent concentration and reaction time) as well as instrumental parameters (e.g. selection of the appropriate RGB model component, ISO, and white balance). The performance of the method was validated, and then verified against conventional spectrofluorimetry, showing comparable accuracy and precision. The method implemented in a smartphone-based device showed a linear range of 0.10–1.00 mgL<sup>-1</sup> with a detection limit of 0.03 mgL<sup>-1</sup>. Synthetic samples were tested to verify analytical usability, followed by analysis of pharmaceutical and urine samples. The method showed good precision (CV<3.4 %) and recovery values (92.3–114.1 %), consistent with spectrofluorimetric results (CV<5.9 %, recovery 92.3–119.8 %). Comparison with other methods highlighted the simplicity and efficiency of the developed approach. The research reveals that it is competitive with existing well-established methods. The proposed device offers reliable results with low reagent and sample consumption, making itsuitable for on-the-go analysis in non-laboratory conditions.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":null,"pages":null},"PeriodicalIF":4.9,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142318511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enzyme-responsive aptasensing based on the ATP aptamer and benzothiazole-coumarin G-quadruplex fluorescent probe for label-free detection of adenosine deaminase activity and its inhibitor 基于 ATP aptamer 和苯并噻唑-香豆素 G-四倍体荧光探针的酶响应式适配传感技术，用于无标记检测腺苷脱氨酶活性及其抑制剂

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2024-09-20 DOI: 10.1016/j.microc.2024.111714

Adenosine deaminase (ADA) is a vital enzyme for regulating the biological process in humans and its abnormal expression level is related to various severe diseases. Nevertheless, conventional approaches for detecting ADA activity are hindered by the drawbacks of complexity and limited detection performances. To address these challenges, we here designed an ADA-responsive aptasensing strategy based on a synthesized benzothiazole-coumarin G-quadruplex (G4) fluorescent probe, which can bind to the G-rich ATP aptamer (G-APA) and form the high-contrast fluorescent complex (S1/G-APA). While the fluorescence of S1/G-APA could be quenched by ATP competition due to the allosteric process, the deamination in ATP catalyzed by ADA would recover the fluorescence of S1/G-APA and allow a facile, turn-on, label-free, and selective detection of ADA activity. This proposed method exhibits favorable detection performances for ADA activity analysis with a wide detection linear range varied from 1 to 100 U/L and a detection limit of 0.82 U/L. Meanwhile, it can be applied for ADA inhibitor (DCF) evaluation and provide a turn-off and sensitive detection performances. Inspired by these detection efficacies, the serum samples spiked with ADA and DCF have been investigated to present the satisfied recovery rates and detection reliability. Collectively, this method will provide a promising way for portable and efficient ADA activity quantification and evaluating the inhibition efficiencies of ADA inhibitors, which hold the potential in promoting ADA-related sensing and pharmaceutical research.

腺苷脱氨酶（ADA）是调节人体生物过程的一种重要酶，其异常表达水平与各种严重疾病有关。然而，检测 ADA 活性的传统方法存在复杂性和检测性能有限等缺点。为了解决这些难题，我们在此设计了一种基于合成的苯并噻唑-香豆素 G-四重链（G4）荧光探针的 ADA 响应型适配体传感策略，该探针可与富含 G 的 ATP 适配体（G-APA）结合并形成高对比度的荧光复合物（S1/G-APA）。由于异构过程，S1/G-APA 的荧光会被 ATP 竞争淬灭，而 ADA 催化的 ATP 脱氨会恢复 S1/G-APA 的荧光，从而实现对 ADA 活性的简便、开启、无标记和选择性检测。该方法的检测线性范围为1-100 U/L，检出限为0.82 U/L。同时，该方法还可用于 ADA 抑制剂（DCF）的评价，并具有关断和灵敏的检测性能。在此基础上，对添加了ADA和DCF的血清样品进行了检测，结果表明回收率和检测可靠性均令人满意。总之，该方法将为便携、高效的 ADA 活性定量和 ADA 抑制剂的抑制效率评估提供一种可行的方法，有望促进 ADA 相关的传感和药物研究。

{"title":"Enzyme-responsive aptasensing based on the ATP aptamer and benzothiazole-coumarin G-quadruplex fluorescent probe for label-free detection of adenosine deaminase activity and its inhibitor","authors":"","doi":"10.1016/j.microc.2024.111714","DOIUrl":"10.1016/j.microc.2024.111714","url":null,"abstract":"<div><div>Adenosine deaminase (ADA) is a vital enzyme for regulating the biological process in humans and its abnormal expression level is related to various severe diseases. Nevertheless, conventional approaches for detecting ADA activity are hindered by the drawbacks of complexity and limited detection performances. To address these challenges, we here designed an ADA-responsive aptasensing strategy based on a synthesized benzothiazole-coumarin G-quadruplex (G4) fluorescent probe, which can bind to the G-rich ATP aptamer (G-APA) and form the high-contrast fluorescent complex (S1/G-APA). While the fluorescence of S1/G-APA could be quenched by ATP competition due to the allosteric process, the deamination in ATP catalyzed by ADA would recover the fluorescence of S1/G-APA and allow a facile, turn-on, label-free, and selective detection of ADA activity. This proposed method exhibits favorable detection performances for ADA activity analysis with a wide detection linear range varied from 1 to 100 U/L and a detection limit of 0.82 U/L. Meanwhile, it can be applied for ADA inhibitor (DCF) evaluation and provide a turn-off and sensitive detection performances. Inspired by these detection efficacies, the serum samples spiked with ADA and DCF have been investigated to present the satisfied recovery rates and detection reliability. Collectively, this method will provide a promising way for portable and efficient ADA activity quantification and evaluating the inhibition efficiencies of ADA inhibitors, which hold the potential in promoting ADA-related sensing and pharmaceutical research.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":null,"pages":null},"PeriodicalIF":4.9,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142319270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Real time monitoring of heavy metal adulteration in biodiesel using Arduino UNO platform@A promising multi-purpose stimuli-responsive azomethine based chemoreceptor for hierarchical tri-ionic sensing 利用 Arduino UNO 平台实时监测生物柴油中的重金属掺假@一种用于分层三离子传感的前景广阔的多用途刺激响应型偶氮金属化学感受器

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2024-09-20 DOI: 10.1016/j.microc.2024.111739

The presence of metallic adulteration even in very trace level may lead to deleterious impact on the biodiesel quality and ultimately may be responsible for the dropping down of system efficiency, which necessitates the trace level recognition of heavy metal adulteration from biodiesel. In this context, herein using an azomethine functionalized chromogenic chemoreceptor, 1-((E)-(4-hydroxyphenylimino) methyl) napthalen-2-ol (HMN) has been reported for the selective chemodosimetric recognition of Cu²⁺ with a lower detection threshold of 7.8 ppb via distinct chromogenic variation of HMN. In addition to this, it has also exhibited selective and reversible naked eye chromogenic sensing behaviour towards F⁻ (LOD=160 ppb) and Al³⁺ (LOD=48 ppb) at very trace level, which is quite lower than the WHO permissible limit. Distinct chromogenic recognition of F⁻ by HMN proceeds via strong intermolecular hydrogen bonding mediated improved intramolecular charge transfer. The spectroscopic response of HMN in alternate presence and absence of the targeted analytes made it suitable to formulate AND-NOT-XNOR-NAND-OR gate based ‘Castle-like’ complicated logic circuitry. In-vitro cell imaging study using A549, human lung carcinoma cell line attests intracellular recognition capability of HMN, demonstrating its effectual bio-medicinal applications. Interestingly, inspired by the capability of HMN towards recognition of copper from biodiesel specimen, an RGB-assisted device comprised of TCS color sensor and an Arduino UNO 8-bit microcontroller has been developed for real-time quantitative analysis of the biodiesel adulteration. Going one step further, utilization of lab-on-a-box based prototype to monitor the concentration-dependent chromogenic fluctuation via RGB analysis is undoubtedly beneficial for the determination of copper adulteration in biodiesel sample. The presently developed chemoreceptor can thus be regarded as a valuable addition in the field of supramolecular chemistry as well as a significant initiative towards regular monitoring of the biodiesel quality control parameters as a part of sustainable environment.

金属掺杂物的存在，即使是非常痕量的掺杂物，也可能会对生物柴油的质量产生有害影响，并最终导致系统效率下降，因此有必要从生物柴油中识别痕量的重金属掺杂物。在此背景下，有报告称使用偶氮甲基官能化色原化学感受器 1-((E)-(4-羟基苯基亚氨基)甲基)萘-2-醇（HMN），通过 HMN 的独特色原变化，以较低的 7.8 ppb 检测阈值选择性识别 Cu2+。此外，它还对痕量水平的 F-（LOD=160ppb）和 Al3+（LOD=48ppb）表现出选择性和可逆性的肉眼显色传感行为，这远低于世界卫生组织的允许限值。HMN 通过强分子间氢键介导的分子内电荷转移对 F- 进行独特的显色识别。HMN 在目标分析物存在和不存在时的光谱响应使其适合于配制基于 "类城堡 "复杂逻辑电路的 AND-NOT-XNOR-NAND-OR 门。使用 A549 人肺癌细胞系进行的体外细胞成像研究证明了 HMN 的细胞内识别能力，证明了其生物医学应用的有效性。有趣的是，受 HMN 识别生物柴油样本中铜的能力的启发，我们开发了一种由 TCS 颜色传感器和 Arduino UNO 8 位微控制器组成的 RGB 辅助设备，用于生物柴油掺假的实时定量分析。更进一步说，利用基于盒上实验室的原型，通过 RGB 分析来监测随浓度变化的色度波动，无疑有利于测定生物柴油样品中的铜掺假情况。因此，目前开发的化学感受器可被视为超分子化学领域的宝贵补充，也是定期监测生物柴油质量控制参数的重要举措，是可持续环境的一部分。

{"title":"Real time monitoring of heavy metal adulteration in biodiesel using Arduino UNO platform@A promising multi-purpose stimuli-responsive azomethine based chemoreceptor for hierarchical tri-ionic sensing","authors":"","doi":"10.1016/j.microc.2024.111739","DOIUrl":"10.1016/j.microc.2024.111739","url":null,"abstract":"<div><div>The presence of metallic adulteration even in very trace level may lead to deleterious impact on the biodiesel quality and ultimately may be responsible for the dropping down of system efficiency, which necessitates the trace level recognition of heavy metal adulteration from biodiesel. In this context, herein using an azomethine functionalized chromogenic chemoreceptor, 1-((E)-(4-hydroxyphenylimino) methyl) napthalen-2-ol (<strong>HMN</strong>) has been reported for the selective chemodosimetric recognition of Cu<sup>2+</sup> with a lower detection threshold of 7.8 ppb <em>via</em> distinct chromogenic variation of <strong>HMN</strong>. In addition to this, it has also exhibited selective and reversible naked eye chromogenic sensing behaviour towards F<sup>−</sup> (LOD=160 ppb) and Al<sup>3+</sup> (LOD=48 ppb) at very trace level, which is quite lower than the WHO permissible limit. Distinct chromogenic recognition of F<sup>−</sup> by <strong>HMN</strong> proceeds <em>via</em> strong intermolecular hydrogen bonding mediated improved intramolecular charge transfer. The spectroscopic response of <strong>HMN</strong> in alternate presence and absence of the targeted analytes made it suitable to formulate AND-NOT-XNOR-NAND-OR gate based ‘<em>Castle-like</em>’ complicated logic circuitry. <em>In-vitro</em> cell imaging study using A549, human lung carcinoma cell line attests intracellular recognition capability of <strong>HMN</strong>, demonstrating its effectual bio-medicinal applications. Interestingly, inspired by the capability of <strong>HMN</strong> towards recognition of copper from biodiesel specimen, an RGB-assisted device comprised of TCS color sensor and an Arduino UNO 8-bit microcontroller has been developed for real-time quantitative analysis of the biodiesel adulteration. Going one step further, utilization of lab-on-a-box based prototype to monitor the concentration-dependent chromogenic fluctuation <em>via</em> RGB analysis is undoubtedly beneficial for the determination of copper adulteration in biodiesel sample. The presently developed chemoreceptor can thus be regarded as a valuable addition in the field of supramolecular chemistry as well as a significant initiative towards regular monitoring of the biodiesel quality control parameters as a part of sustainable environment.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":null,"pages":null},"PeriodicalIF":4.9,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142322199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Peptide-based fluorescent probe with large Stokes shift for specific sequential detection of copper (II) ions and histidine: Smartphone device, real samples, test strips and bioimaging applications 基于多肽的荧光探针具有大斯托克斯位移，可用于特异性连续检测铜 (II) 离子和组氨酸：智能手机设备、真实样品、试纸和生物成像应用

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2024-09-20 DOI: 10.1016/j.microc.2024.111728

A novel and facile fluorescent probe, DNH, was developed based on a dipeptide backbone (Asn-His-NH₂) labelled with a dansyl fluorophore. DNH exhibited significant advantages in detecting Cu²⁺ based on the paramagnetic quenching mechanism, including satisfactory water solubility (100 % aqueous medium), a large Stokes shift (230 nm), excellent anti-interference capability (no interference), a low detection limit (19.6 nM), rapid response time (within 30 s) and a wide pH range (7–12). Employing a displacement approach, the DNH-Cu²⁺ ensemble showed strong fluorescence enhancement upon recognition of _L-His with outstanding selectivity, and the detection limit for the DNH-Cu²⁺ ensemble with respect to _L-His was calculated at 22.8 nM. Notably, DNH demonstrated favourable reversibility for at least seven cycles with minor changes in fluorescence intensity when Cu²⁺ and _L-His were added alternately. Moreover, DNH exhibited excellent low cytotoxicity and good biocompatibility, and was utilised as an outstanding probe for the sequential determination of Cu²⁺ and _L-His in living cells and zebrafish. DNH was successfully used to determine Cu²⁺ and _L-His in three actual water samples with good recovery and accuracy. In addition, DNH was applied to filter paper to develop visual test strips for the rapid and high-efficiency analysis of Cu²⁺ and _L-His. Furthermore, the semi-quantitative visual monitoring of Cu²⁺ and _L-His was achieved by developing a smartphone colour recogniser into a portable analytical device, achieving low detection limits of 0.49 μM and 0.63 μM, respectively. Given these attributes, DNH presents itself as a promising peptide-based fluorescent probe, showcasing considerable potential for real-time visual monitoring of Cu²⁺ and _L-His in various environmental and biological settings.

基于丹酰荧光团标记的二肽骨架（Asn-His-NH2），开发出了一种新型简便的荧光探针 DNH。基于顺磁淬灭机制，DNH 在检测 Cu2+ 方面表现出明显的优势，包括令人满意的水溶性（100% 水介质）、较大的斯托克斯位移（230 nm）、出色的抗干扰能力（无干扰）、较低的检测限（19.6 nM）、快速响应时间（30 秒内）和较宽的 pH 值范围（7-12）。采用置换方法，DNH-Cu2+ 组合在识别 L-His 时显示出强烈的荧光增强，具有出色的选择性，DNH-Cu2+ 组合对 L-His 的检测限为 22.8 nM。值得注意的是，当交替添加 Cu2+ 和 L-His 时，DNH 显示出良好的可逆性，至少可持续七个周期，且荧光强度变化不大。此外，DNH 还具有极低的细胞毒性和良好的生物相容性，可作为一种出色的探针，用于在活细胞和斑马鱼中连续测定 Cu2+ 和 L-His。DNH 成功用于测定三种实际水样中的 Cu2+ 和 L-His，回收率高，准确度好。此外，还将 DNH 应用于滤纸，开发出了快速高效分析 Cu2+ 和 L-His 的可视试纸。此外，通过将智能手机的颜色识别器开发成便携式分析设备，实现了对 Cu2+ 和 L-His 的半定量视觉监测，检出限分别低至 0.49 μM 和 0.63 μM。鉴于这些特性，DNH 是一种很有前途的肽基荧光探针，在各种环境和生物环境中对 Cu2+ 和 L-His 进行实时可视监测方面具有很大的潜力。

{"title":"Peptide-based fluorescent probe with large Stokes shift for specific sequential detection of copper (II) ions and histidine: Smartphone device, real samples, test strips and bioimaging applications","authors":"","doi":"10.1016/j.microc.2024.111728","DOIUrl":"10.1016/j.microc.2024.111728","url":null,"abstract":"<div><div>A novel and facile fluorescent probe, <strong>DNH</strong>, was developed based on a dipeptide backbone (Asn-His-NH<sub>2</sub>) labelled with a dansyl fluorophore. <strong>DNH</strong> exhibited significant advantages in detecting Cu<sup>2+</sup> based on the paramagnetic quenching mechanism, including satisfactory water solubility (100 % aqueous medium), a large Stokes shift (230 nm), excellent anti-interference capability (no interference), a low detection limit (19.6 nM), rapid response time (within 30 s) and a wide pH range (7–12). Employing a displacement approach, the <strong>DNH</strong>-Cu<sup>2+</sup> ensemble showed strong fluorescence enhancement upon recognition of <sub>L</sub>-His with outstanding selectivity, and the detection limit for the <strong>DNH</strong>-Cu<sup>2+</sup> ensemble with respect to <sub>L</sub>-His was calculated at 22.8 nM. Notably, <strong>DNH</strong> demonstrated favourable reversibility for at least seven cycles with minor changes in fluorescence intensity when Cu<sup>2+</sup> and <sub>L</sub>-His were added alternately. Moreover, <strong>DNH</strong> exhibited excellent low cytotoxicity and good biocompatibility, and was utilised as an outstanding probe for the sequential determination of Cu<sup>2+</sup> and <sub>L</sub>-His in living cells and zebrafish. <strong>DNH</strong> was successfully used to determine Cu<sup>2+</sup> and <sub>L</sub>-His in three actual water samples with good recovery and accuracy. In addition, <strong>DNH</strong> was applied to filter paper to develop visual test strips for the rapid and high-efficiency analysis of Cu<sup>2+</sup> and <sub>L</sub>-His. Furthermore, the semi-quantitative visual monitoring of Cu<sup>2+</sup> and <sub>L</sub>-His was achieved by developing a smartphone colour recogniser into a portable analytical device, achieving low detection limits of 0.49 μM and 0.63 μM, respectively. Given these attributes, <strong>DNH</strong> presents itself as a promising peptide-based fluorescent probe, showcasing considerable potential for real-time visual monitoring of Cu<sup>2+</sup> and <sub>L</sub>-His in various environmental and biological settings.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":null,"pages":null},"PeriodicalIF":4.9,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142310712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A focused review on organic electrochemical transistors: A potential futuristic technological application in microelectronics 聚焦有机电化学晶体管：微电子学中一种潜在的未来技术应用

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2024-09-20 DOI: 10.1016/j.microc.2024.111737

Background

An organic electrochemical transistor (OECT) is an important device in an area of the art in bioelectronics that can convert ionic and biological inputs into electronic outputs with its aqueous environment. The OECTs use a combination of electronic and ionic charges to leverage the mixed conduction properties of materials within their channel. As bioelectronics applications become popular in OECTs, there is a need to standardize the material channel mixed insulation characteristics, however there is some variation in conditions. This review provides an in-depth formulation of OECTs, including their selection mechanisms, use of organic materials, fabrication methods, and applications to bioelectronics devices. Moreover, it offers a critical assessment of OECT’s research and development in the future.

Results

The review is organized as follows: First, we provide an overview of the construction and operation of an OECT device in section 2. Next, in section 3, we describe how OECT behavior differs from conventional metal oxide semiconductor field effect transistor (MOSFET), and we discuss how electronic charge transport in the channel by Bernard’s Model. In section 4, we discuss several materials for the transient behavior of OECTs. In section 5, we deal with the recent advances in OECT device fabrication. Then, in section 6, we consider the current applications of OECTs in the fields of biosensing, circuits and logic, neuromorphic computing, and prosthetic and human–machine interfaces. In section 7, we describe limitations concerning the OECT device with expert suggestions to address these limitations. Finally, in section 8, we close the Review with a brief conclusion.

Significance

A significant advancement has been made in this field through the development of innovative approaches, such as spray coating for uniform channel deposition and screen printing for large-scale production. The full potential of OECTs in revolutionizing bioelectronics and healthcare applications will be realized only through collaborative efforts between researchers, engineers, and industry stakeholders.

背景有机电化学晶体管（OECT）是生物电子学技术领域的一个重要装置，它能利用水环境将离子和生物输入转化为电子输出。OECT 结合使用电子和离子电荷，充分利用通道内材料的混合传导特性。随着生物电子学应用在 OECTs 中的普及，有必要对材料通道的混合绝缘特性进行标准化，但条件存在一定差异。本综述深入阐述了 OECTs，包括其选择机制、有机材料的使用、制造方法以及在生物电子器件中的应用。此外，它还对 OECT 未来的研究和发展进行了批判性评估：首先，我们在第 2 部分概述了 OECT 设备的构造和运行。接下来，在第 3 节中，我们介绍了 OECT 行为与传统金属氧化物半导体场效应晶体管 (MOSFET) 的不同之处，并通过伯纳德模型讨论了电子电荷如何在沟道中传输。在第 4 节中，我们讨论了 OECT 瞬态行为的几种材料。在第 5 节中，我们讨论了 OECT 器件制造的最新进展。然后，在第 6 节中，我们探讨了 OECT 目前在生物传感、电路和逻辑、神经形态计算以及假肢和人机界面等领域的应用。在第 7 节中，我们介绍了 OECT 设备的局限性，并提出了解决这些局限性的专家建议。最后，在第 8 节中，我们以简短的结论结束本综述。重要意义通过开发创新方法，如用于均匀沟道沉积的喷涂和用于大规模生产的丝网印刷，该领域已取得重大进展。只有通过研究人员、工程师和行业利益相关者的通力合作，才能充分发挥 OECTs 在革新生物电子学和医疗保健应用方面的潜力。

{"title":"A focused review on organic electrochemical transistors: A potential futuristic technological application in microelectronics","authors":"","doi":"10.1016/j.microc.2024.111737","DOIUrl":"10.1016/j.microc.2024.111737","url":null,"abstract":"<div><h3>Background</h3><div>An organic electrochemical transistor (OECT) is an important device in an area of the art in bioelectronics that can convert ionic and biological inputs into electronic outputs with its aqueous environment. The OECTs use a combination of electronic and ionic charges to leverage the mixed conduction properties of materials within their channel. As bioelectronics applications become popular in OECTs, there is a need to standardize the material channel mixed insulation characteristics, however there is some variation in conditions. This review provides an in-depth formulation of OECTs, including their selection mechanisms, use of organic materials, fabrication methods, and applications to bioelectronics devices. Moreover, it offers a critical assessment of OECT’s research and development in the future.</div></div><div><h3>Results</h3><div>The review is organized as follows: First, we provide an overview of the construction and operation of an OECT device in section 2. Next, in section 3, we describe how OECT behavior differs from conventional metal oxide semiconductor field effect transistor (MOSFET), and we discuss how electronic charge transport in the channel by Bernard’s Model. In section 4, we discuss several materials for the transient behavior of OECTs. In section 5, we deal with the recent advances in OECT device fabrication. Then, in section 6, we consider the current applications of OECTs in the fields of biosensing, circuits and logic, neuromorphic computing, and prosthetic and human–machine interfaces. In section 7, we describe limitations concerning the OECT device with expert suggestions to address these limitations. Finally, in section 8, we close the Review with a brief conclusion.</div></div><div><h3>Significance</h3><div>A significant advancement has been made in this field through the development of innovative approaches, such as spray coating for uniform channel deposition and screen printing for large-scale production. The full potential of OECTs in revolutionizing bioelectronics and healthcare applications will be realized only through collaborative efforts between researchers, engineers, and industry stakeholders.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":null,"pages":null},"PeriodicalIF":4.9,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142317836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Selective separation of polyphenols with a novel azo porous organic polymer: Ultrahigh adsorption capacity and superfast adsorption rate 利用新型偶氮多孔有机聚合物选择性分离多酚：超高吸附容量和超快吸附速率

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2024-09-20 DOI: 10.1016/j.microc.2024.111696

For the first time, a novel porous organic polymer (POP), namely Cin-POP, was constructed under normal temperature and pressure using less toxic basic fuchsin and natural cinnamic acid. Cin-POP was synthesized through the diazo-coupling reaction between the diazonium salts of basic fuchsin and cinnamic acid. The FT-IR spectra and XPS analysis prove the successful construction of the Cin-POP, and the BET analysis demonstrates the high-surface-area (166.42–202.56 m² g⁻¹) and suitable pore sizes (11.00–18.63 nm). Owing to the advantages of porous structures, abundant aromatic groups, good thermal stability, and good dispersity, Cin-POP exhibits remarkable adsorption rates and capacities towards polyphenols, including procyanidine and rutin. Especially for procyanidine, the extraction efficiency can arrive at nearly 98.1 % after 2.5 min adsorption, and the pseudo-second-order rate constant (k₂) of Cin-POP is 0.0257 g mg⁻¹ min⁻¹. The maximum adsorption capacities of Cin-POP towards procyanidine and rutin are 2500.00 mg g⁻¹ and 1814.83 mg g⁻¹, outpacing all reported adsorbents. In addition, Cin-POP can selectively adsorb polyphenol among the mixtures of polyphenols and alkaloids. More importantly, Cin-POP can be used for polyphenols separation from complex cinnamon extract. All the above advantages make Cin-POP comparable porous adsorbent for polyphenol separation.

利用毒性较低的碱性紫苏和天然肉桂酸，首次在常温常压下构建了一种新型多孔有机聚合物（POP），即 Cin-POP。Cin-POP 是通过碱性品红的重氮盐与肉桂酸的重氮偶联反应合成的。傅立叶变换红外光谱和 XPS 分析证明了 Cin-POP 的成功构建，BET 分析表明了其高比表面积（166.42-202.56 m2 g-1）和合适的孔径（11.00-18.63 nm）。由于具有多孔结构、丰富的芳香基团、良好的热稳定性和分散性等优点，Cin-POP 对包括原花青素和芦丁在内的多酚具有显著的吸附率和吸附容量。特别是对原花青素，吸附 2.5 分钟后萃取效率可达近 98.1%，Cin-POP 的假二阶速率常数（k2）为 0.0257 g mg-1 min-1。Cin-POP 对原花青素和芦丁的最大吸附容量分别为 2500.00 mg g-1 和 1814.83 mg g-1，超过了所有已报道的吸附剂。此外，Cin-POP 还能选择性地吸附多酚和生物碱混合物中的多酚。更重要的是，Cin-POP 可用于从复杂的肉桂提取物中分离多酚。所有这些优点使 Cin-POP 成为可用于多酚分离的多孔吸附剂。

{"title":"Selective separation of polyphenols with a novel azo porous organic polymer: Ultrahigh adsorption capacity and superfast adsorption rate","authors":"","doi":"10.1016/j.microc.2024.111696","DOIUrl":"10.1016/j.microc.2024.111696","url":null,"abstract":"<div><div>For the first time, a novel porous organic polymer (POP), namely Cin-POP, was constructed under normal temperature and pressure using less toxic basic fuchsin and natural cinnamic acid. Cin-POP was synthesized through the diazo-coupling reaction between the diazonium salts of basic fuchsin and cinnamic acid. The FT-IR spectra and XPS analysis prove the successful construction of the Cin-POP, and the BET analysis demonstrates the high-surface-area (166.42–202.56 m<sup>2</sup> g<sup>−1</sup>) and suitable pore sizes (11.00–18.63 nm). Owing to the advantages of porous structures, abundant aromatic groups, good thermal stability, and good dispersity, Cin-POP exhibits remarkable adsorption rates and capacities towards polyphenols, including procyanidine and rutin. Especially for procyanidine, the extraction efficiency can arrive at nearly 98.1 % after 2.5 min adsorption, and the pseudo-second-order rate constant (k<sub>2</sub>) of Cin-POP is 0.0257 g mg<sup>−1</sup> min<sup>−1</sup>. The maximum adsorption capacities of Cin-POP towards procyanidine and rutin are 2500.00 mg g<sup>−1</sup> and 1814.83 mg g<sup>−1</sup>, outpacing all reported adsorbents. In addition, Cin-POP can selectively adsorb polyphenol among the mixtures of polyphenols and alkaloids. More importantly, Cin-POP can be used for polyphenols separation from complex cinnamon extract. All the above advantages make Cin-POP comparable porous adsorbent for polyphenol separation.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":null,"pages":null},"PeriodicalIF":4.9,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142310715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hemoglobin-Capped carbon dots synthesized via microwave green approach as a biosensor for specific cholesterol detection 通过微波绿色方法合成的血红蛋白封接碳点作为特异性胆固醇检测生物传感器

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2024-09-20 DOI: 10.1016/j.microc.2024.111652

This study explores the potential of hemoglobin-capped green-synthesized carbon dots (g-CD) as a biosensor for the specific detection of cholesterol. The CDs, synthesized using an environmentally friendly and convenient microwave green approach, derived from Wood Extract of Wild Cherry Shrub and utilizing hemoglobin as a capping agent, offer promising characteristics for cholesterol detection. This green synthesis method avoids the use of toxic precursors, making the process safer and more sustainable. Additionally, microwave (MW) heating, being volumetric in nature, reduces the synthesis time and energy consumption, resulting in a uniform and unique microstructure, offering a substantial advantage over traditional methods. Through fluorescence spectroscopy, the interaction between the g-CD/Hb complex and cholesterol was investigated. The results demonstrate that the fluorescence intensity of the g-CD/Hb complex increases with the concentration of cholesterol, suggesting a potential application for cholesterol detection. Notably, the calculated limit of detection (LOD) was found to be 9.22 μM, significantly lower than previously reported sensors, indicating higher sensitivity. Moreover, the biosensor exhibits high selectivity towards cholesterol over other biomolecules commonly found in human serum. Additionally, the biosensor shows excellent performance with real human blood samples, indicating its practical utility in clinical settings. Overall, the g-CD/Hb biosensor presents a promising approach for the specific detection of cholesterol, offering potential applications in biomedical research and clinical diagnostics. The key innovations include the use of a green and rapid synthesis method, improved detection limit, and validation of performance in real samples, distinguishing this sensor from previous research.

本研究探讨了血红蛋白封端的绿色合成碳点（g-CD）作为生物传感器特异性检测胆固醇的潜力。这种碳点采用环保、便捷的微波绿色方法合成，提取自野生樱桃树的木质提取物，并利用血红蛋白作为封盖剂，具有检测胆固醇的良好特性。这种绿色合成方法避免了有毒前体的使用，使工艺更安全、更可持续。此外，微波（MW）加热具有容积性质，可减少合成时间和能耗，从而获得均匀独特的微观结构，与传统方法相比具有很大优势。通过荧光光谱，研究了 g-CD/Hb 复合物与胆固醇之间的相互作用。结果表明，g-CD/Hb 复合物的荧光强度随胆固醇浓度的增加而增加，这表明它有可能应用于胆固醇检测。值得注意的是，计算得出的检测限（LOD）为 9.22 μM，明显低于之前报道的传感器，这表明该传感器具有更高的灵敏度。此外，该生物传感器对胆固醇的选择性高于人血清中常见的其他生物大分子。此外，该生物传感器在真实人体血液样本中表现出卓越的性能，表明它在临床环境中具有实用性。总之，g-CD/Hb 生物传感器为胆固醇的特异性检测提供了一种前景广阔的方法，有望应用于生物医学研究和临床诊断。该传感器的主要创新点包括采用绿色快速合成方法、提高检测限以及在真实样本中进行性能验证，从而使其有别于以往的研究。

{"title":"Hemoglobin-Capped carbon dots synthesized via microwave green approach as a biosensor for specific cholesterol detection","authors":"","doi":"10.1016/j.microc.2024.111652","DOIUrl":"10.1016/j.microc.2024.111652","url":null,"abstract":"<div><div>This study explores the potential of hemoglobin-capped green-synthesized carbon dots (g-CD) as a biosensor for the specific detection of cholesterol. The CDs, synthesized using an environmentally friendly and convenient microwave green approach, derived from Wood Extract of Wild Cherry Shrub and utilizing hemoglobin as a capping agent, offer promising characteristics for cholesterol detection. This green synthesis method avoids the use of toxic precursors, making the process safer and more sustainable. Additionally, microwave (MW) heating, being volumetric in nature, reduces the synthesis time and energy consumption, resulting in a uniform and unique microstructure, offering a substantial advantage over traditional methods. Through fluorescence spectroscopy, the interaction between the g-CD/Hb complex and cholesterol was investigated. The results demonstrate that the fluorescence intensity of the g-CD/Hb complex increases with the concentration of cholesterol, suggesting a potential application for cholesterol detection. Notably, the calculated limit of detection (LOD) was found to be 9.22 μM, significantly lower than previously reported sensors, indicating higher sensitivity. Moreover, the biosensor exhibits high selectivity towards cholesterol over other biomolecules commonly found in human serum. Additionally, the biosensor shows excellent performance with real human blood samples, indicating its practical utility in clinical settings. Overall, the g-CD/Hb biosensor presents a promising approach for the specific detection of cholesterol, offering potential applications in biomedical research and clinical diagnostics. The key innovations include the use of a green and rapid synthesis method, improved detection limit, and validation of performance in real samples, distinguishing this sensor from previous research.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":null,"pages":null},"PeriodicalIF":4.9,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142310600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

AuNP@Self-assembled 4H-Chromene-Enhanced colorimetric detection of ganciclovir using smartphone Technology: Portable solutions for healthcare 利用智能手机技术对AuNP@自组装的4H-Chromene-增强型更昔洛韦进行比色检测：便携式医疗解决方案

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2024-09-20 DOI: 10.1016/j.microc.2024.111734

Any substance, radiation, or radionuclide in our environment that promotes carcinogenesis is a cancerogen. In the current work, we modified the 4H-chromene derivative by covering its surface with a shell of gold nanoparticles. Interestingly, ganciclovir (GCV) formed a bridge between the Nap-group of 4H-chromene and gold nanoparticles to display surface charge reduction properties in AuNP@Self-assembled 4H-Chromene. The surface charge of AuNPs is reduced as a result of the electron transfer mechanism brought on by the creation of the Nap-GCV-AuNP link, which leads to agglomeration. This led to the development of a dual-mode colorimetric sensor for GCV that incorporates solution form and portable sensor strip. The probe showed a linear range of 0–74 nM under ideal circumstances, with a detection limit of 4 nM in solution and 3 nM on paper strip detection. An evaluation of the practical utility in human serum using a portable visual read-out produced satisfactory findings, with a recovery percentage of 102.1 % and a standard deviation of ± 4 %. Surprisingly, this is the first work on the colorimetric detection of Ganciclovir that we are aware of.

在我们的环境中，任何促进致癌的物质、辐射或放射性核素都是致癌物质。在目前的工作中，我们通过在 4H-chromene 衍生物的表面覆盖一层金纳米粒子壳对其进行了改性。有趣的是，更昔洛韦（Ganciclovir，GCV）在 4H-Chromene的Nap基团和金纳米粒子之间形成了桥梁，从而在AuNP@Self-assembled 4H-Chromene中显示出表面电荷还原特性。由于 Nap-GCV-AuNP 链接产生的电子转移机制，AuNPs 的表面电荷减少，从而导致团聚。因此，我们开发出了一种双模式的 GCV 比色传感器，既有溶液形式，又有便携式传感器条。该探针在理想情况下的线性范围为 0-74 nM，溶液检测限为 4 nM，纸条检测限为 3 nM。使用便携式可视读数器对人体血清中的实际应用进行评估后，结果令人满意，回收率为 102.1%，标准偏差为 ± 4%。令人惊讶的是，这是我们所知的第一项关于更昔洛韦比色检测的工作。

{"title":"AuNP@Self-assembled 4H-Chromene-Enhanced colorimetric detection of ganciclovir using smartphone Technology: Portable solutions for healthcare","authors":"","doi":"10.1016/j.microc.2024.111734","DOIUrl":"10.1016/j.microc.2024.111734","url":null,"abstract":"<div><div>Any substance, radiation, or radionuclide in our environment that promotes carcinogenesis is a cancerogen. In the current work, we modified the <em>4H</em>-chromene derivative by covering its surface with a shell of gold nanoparticles. Interestingly, ganciclovir (GCV) formed a bridge between the Nap-group of <em>4H</em>-chromene and gold nanoparticles to display surface charge reduction properties in <strong>AuNP@Self-assembled <em>4H</em>-Chromene</strong>. The surface charge of AuNPs is reduced as a result of the electron transfer mechanism brought on by the creation of the Nap-GCV-AuNP link, which leads to agglomeration. This led to the development of a dual-mode colorimetric sensor for GCV that incorporates solution form and portable sensor strip. The probe showed a linear range of 0–74 nM under ideal circumstances, with a detection limit of 4 nM in solution and 3 nM on paper strip detection. An evaluation of the practical utility in human serum using a portable visual read-out produced satisfactory findings, with a recovery percentage of 102.1 % and a standard deviation of ± 4 %. Surprisingly, this is the first work on the colorimetric detection of Ganciclovir that we are aware of.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":null,"pages":null},"PeriodicalIF":4.9,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142310711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Real-time detection of cerebral edema in mice based on low-field nuclear magnetic resonance 基于低场核磁共振的小鼠脑水肿实时检测

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2024-09-20 DOI: 10.1016/j.microc.2024.111738

Cerebral edema is a secondary symptom of several conditions, including stroke, liver, failure, and severe craniocerebral injury. This study aimed to propose a Low-Field Nuclear Magnetic Resonance (LF-NMR) method for rapid and nondestructive measurement of brain water content and water phase state evaluating animal brain edema models. The water transverse relaxation time (T₂) distribution in brain tissues was ascertained by measuring the T₂ spectrograms of mouse brain tissues following various drying durations, in accordance with the shifting law of T₂ peaks. A methodological study was carried out for the determination of water content in mouse brain tissue by the LF-NMR Peak Area (LF-NMR-PA) method using CuSO₄ solution as standard solution. This method was studied in comparison with the wet/dry weight method and the Partial Least Squares Regression (PLSR) prediction method in normal and MCAO model mice, and further applied to the study of mannitol treatment of cerebral edema to validate the precision and accuracy of the hydration measurements as well as the method’s impact on subsequent experiments of cerebral infarct area measurement. In the T₂ spectra of mouse brain tissues, the separate peaks T₂₁ (0–10 ms), T₂₂ (10–100 ms), and T₂₃ (100–1000 ms) correspond to fat and bound water, water, and free water in brain tissue, respectively. The LF-NMR-PA method had good specificity, linearity, precision, stability, reproducibility, and recovery. The accuracy and range of use of the LF-NMR-PA method were better than those of the PLSR method, and its results were not significantly different from those of the wet/dry weight method. However, the short measurement time of the LF-NMR-PA method ensured the integrity of the tissues, and the determination of the moisture by this method did not have a significant effect on the subsequent determination of the area of cerebral infarction, which ensured the consistency of the experimental results.

脑水肿是中风、肝功能衰竭和严重颅脑损伤等多种疾病的继发症状。本研究旨在提出一种低场核磁共振（LF-NMR）方法，用于快速、无损地测量动物脑水肿模型的脑水含量和水相状态。根据 T2 峰移动规律，通过测量小鼠脑组织在不同干燥持续时间后的 T2 频谱图，确定了脑组织中水的横向弛豫时间（T2）分布。以 CuSO4 溶液为标准溶液，采用低频核磁共振峰面积（LF-NMR-PA）法测定了小鼠脑组织中的水分含量。该方法在正常小鼠和 MCAO 模型小鼠中与干湿重量法和部分最小二乘法回归预测法进行了比较研究，并进一步应用于甘露醇治疗脑水肿的研究，以验证水合测量的精确性和准确性以及该方法对后续脑梗塞面积测量实验的影响。在小鼠脑组织的 T2 光谱中，独立的峰 T21（0-10 ms）、T22（10-100 ms）和 T23（100-1000 ms）分别对应脑组织中的脂肪和结合水、水和自由水。LF-NMR-PA 方法具有良好的特异性、线性、精密度、稳定性、重现性和回收率。LF-NMR-PA 法的准确度和使用范围均优于 PLSR 法，其结果与湿重/干重法没有显著差异。但是，LF-NMR-PA 法测量时间短，保证了组织的完整性，而且该方法测定水分对后续测定脑梗死面积没有明显影响，保证了实验结果的一致性。

{"title":"Real-time detection of cerebral edema in mice based on low-field nuclear magnetic resonance","authors":"","doi":"10.1016/j.microc.2024.111738","DOIUrl":"10.1016/j.microc.2024.111738","url":null,"abstract":"<div><div>Cerebral edema is a secondary symptom of several conditions, including stroke, liver, failure, and severe craniocerebral injury. This study aimed to propose a Low-Field Nuclear Magnetic Resonance (LF-NMR) method for rapid and nondestructive measurement of brain water content and water phase state evaluating animal brain edema models. The water transverse relaxation time (<em>T<sub>2</sub></em>) distribution in brain tissues was ascertained by measuring the <em>T<sub>2</sub></em> spectrograms of mouse brain tissues following various drying durations, in accordance with the shifting law of <em>T<sub>2</sub></em> peaks. A methodological study was carried out for the determination of water content in mouse brain tissue by the LF-NMR Peak Area (LF-NMR-PA) method using CuSO<sub>4</sub> solution as standard solution. This method was studied in comparison with the wet/dry weight method and the Partial Least Squares Regression (PLSR) prediction method in normal and MCAO model mice, and further applied to the study of mannitol treatment of cerebral edema to validate the precision and accuracy of the hydration measurements as well as the method’s impact on subsequent experiments of cerebral infarct area measurement. In the <em>T<sub>2</sub></em> spectra of mouse brain tissues, the separate peaks <em>T<sub>21</sub></em> (0–10 ms), <em>T<sub>22</sub></em> (10–100 ms), and <em>T<sub>23</sub></em> (100–1000 ms) correspond to fat and bound water, water, and free water in brain tissue, respectively. The LF-NMR-PA method had good specificity, linearity, precision, stability, reproducibility, and recovery. The accuracy and range of use of the LF-NMR-PA method were better than those of the PLSR method, and its results were not significantly different from those of the wet/dry weight method. However, the short measurement time of the LF-NMR-PA method ensured the integrity of the tissues, and the determination of the moisture by this method did not have a significant effect on the subsequent determination of the area of cerebral infarction, which ensured the consistency of the experimental results.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":null,"pages":null},"PeriodicalIF":4.9,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142318509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0