Microchemical Journal最新文献_第7页

Early detection for carbohydrate antigen-19-9 based on surface enhanced Raman spectroscopy aptamer sensor 基于表面增强拉曼光谱适配体传感器的碳水化合物抗原-19-9 早期检测技术

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2024-09-22 DOI: 10.1016/j.microc.2024.111750

Ji Xia , Yan Li , Yue Xin , Luyao Kang , Dan Lu

Background

Incipient screening for cervical cancer is crucial for treatment and improving prognosis. In this work, SERS based aptamer sensor (aptasensor) has been developed and applied to high-sensitivity detection of cervical cancer tumor marker (Carbohydrate antigen19-9 (CA19-9)) with a microporous chip, which was used as surface enhanced Raman scattering (SERS) analysis platform.

Methods

The aptamer of CA19-9 was immobilized on the gold nanodumbbell as a capture probe and the gold nanobipyramid containing complementary aptamer chains of the signal molecule Cy5 was modified as a signal probe. SERS aptasensor was constructed by combining signal probes and capture probes. It caused signal molecule Cy5 were sandwiched between the signal probes and capture probes; the constructed SERS aptasensor generated a strong SERS signal of Cy5. Based on the strong specificity of the aptamer, the CA19-9 aptamer binds specifically to CA19-9 when CA19-9 exist in the detection environment. The signal probe was released, resulting in a decrease in the SERS signal of the detection environment.

Results

Under the optimal detection conditions, the detection limit of the aptasensor was calculated to be 1.16 × 10⁻³ U/mL and limit of quantification is equal to 3.87 × 10⁻³ U/mL. In addition, a standard cervical cancer bearing mouse model was constructed and the SERS spectra of the serum of mice at different stages were measured. The CA19-9 content in the serum of mice at different stages was calculated. Compared with the results of enzyme-linked immunosorbent assay (ELISA), relative error (RE) values (2.54 %, −6.093 %, −4.922 %, and 3.04 %) of the CA19-9 concentrations detected by the two methods on the day of 0, 7, 14 and 28.

Conclusion

The proposed SERS aptasensor provides a new and reliable platform for early cervical cancer screening, and its low RE value proves that it will be a promising CA19-9 detection method.

背景宫颈癌的早期筛查对于治疗和改善预后至关重要。本研究开发了基于 SERS 的适配体传感器（aptasensor），并将其应用于宫颈癌肿瘤标志物（碳水化合物抗原 19-9 (CA19-9)）的高灵敏度检测，其微孔芯片被用作表面增强拉曼散射（SERS）分析平台。方法将 CA19-9 的适配体固定在金纳米哑铃上作为捕获探针，将含有信号分子 Cy5 的互补适配体链的金纳米双锥体修饰成信号探针。通过信号探针和捕获探针的组合，构建了 SERS 准传感器。信号分子Cy5被夹在信号探针和捕获探针之间，构建的SERS适配传感器产生了强烈的Cy5 SERS信号。基于适配体的强特异性，当CA19-9存在于检测环境中时，CA19-9适配体会与CA19-9发生特异性结合。结果在最佳检测条件下，计算出该适配体的检测限为 1.16 × 10-3 U/mL，定量限为 3.87 × 10-3 U/mL。此外，还构建了标准宫颈癌小鼠模型，并测定了不同阶段小鼠血清的 SERS 光谱。计算了不同阶段小鼠血清中 CA19-9 的含量。与酶联免疫吸附试验（ELISA）的结果相比，两种方法在0、7、14和28天检测到的CA19-9浓度的相对误差（RE）值分别为2.54%、-6.093%、-4.922%和3.04%。结论：所提出的SERS灵敏传感器为早期宫颈癌筛查提供了一个新的、可靠的平台，其较低的RE值证明它将是一种有前途的CA19-9检测方法。

{"title":"Early detection for carbohydrate antigen-19-9 based on surface enhanced Raman spectroscopy aptamer sensor","authors":"Ji Xia , Yan Li , Yue Xin , Luyao Kang , Dan Lu","doi":"10.1016/j.microc.2024.111750","DOIUrl":"10.1016/j.microc.2024.111750","url":null,"abstract":"<div><h3>Background</h3><div>Incipient screening for cervical cancer is crucial for treatment and improving prognosis. In this work, SERS based aptamer sensor (aptasensor) has been developed and applied to high-sensitivity detection of cervical cancer tumor marker (Carbohydrate antigen19-9 (CA19-9)) with a microporous chip, which was used as surface enhanced Raman scattering (SERS) analysis platform.</div></div><div><h3>Methods</h3><div>The aptamer of CA19-9 was immobilized on the gold nanodumbbell as a capture probe and the gold nanobipyramid containing complementary aptamer chains of the signal molecule Cy5 was modified as a signal probe. SERS aptasensor was constructed by combining signal probes and capture probes. It caused signal molecule Cy5 were sandwiched between the signal probes and capture probes; the constructed SERS aptasensor generated a strong SERS signal of Cy5. Based on the strong specificity of the aptamer, the CA19-9 aptamer binds specifically to CA19-9 when CA19-9 exist in the detection environment. The signal probe was released, resulting in a decrease in the SERS signal of the detection environment.</div></div><div><h3>Results</h3><div>Under the optimal detection conditions, the detection limit of the aptasensor was calculated to be 1.16 × 10<sup>−3</sup> U/mL and limit of quantification is equal to 3.87 × 10<sup>−3</sup> U/mL. In addition, a standard cervical cancer bearing mouse model was constructed and the SERS spectra of the serum of mice at different stages were measured. The CA19-9 content in the serum of mice at different stages was calculated. Compared with the results of enzyme-linked immunosorbent assay (ELISA), relative error (RE) values (2.54 %, −6.093 %, −4.922 %, and 3.04 %) of the CA19-9 concentrations detected by the two methods on the day of 0, 7, 14 and 28.</div></div><div><h3>Conclusion</h3><div>The proposed SERS aptasensor provides a new and reliable platform for early cervical cancer screening, and its low RE value proves that it will be a promising CA19-9 detection method.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"207 ","pages":"Article 111750"},"PeriodicalIF":4.9,"publicationDate":"2024-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142315002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Determination of proteins in hypoxic Müller glia cells by single-cell ICP-MS: The use of a Rh-DNA intercalator for enhanced cell detection 利用单细胞 ICP-MS 测定缺氧 Müller 胶质细胞中的蛋白质：使用 Rh-DNA 中间体增强细胞检测能力

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2024-09-22 DOI: 10.1016/j.microc.2024.111745

Alicia Villa-Vázquez , Paula Menero-Valdés , Lydia Álvarez , Héctor González-Iglesias , Beatriz Fernández , Rosario Pereiro

Single cell − inductively coupled plasma-mass spectrometry (sc-ICP-MS) enables the identification and quantification of elements and biomolecules within individual cells. Target proteins can be analyzed by sc-ICP-MS using metal nanoclusters (MNCs)-based immunoprobes, facilitating the detection of low amounts of proteins in each cell. However, for the full implementation of sc-ICP-MS, an effective strategy to enhance the detection of cellular events is required. In this work, the measurement of endogenous elements and two external tags (ruthenium red and Rh-DNA intercalator) was investigated for cell detection. As a proof of concept, the sequential determination of two proteins (hypoxia inducible factor-1α and vascular endothelial growth factor; HIF-1α and VEGF, respectively) in individual human Müller glia cells (MIO-M1) was pursued. Specific antibodies against HIF-1α and VEGF were labelled with AuNCs and IrNCs, respectively. The results demonstrated the advantages of using Rh-DNA intercalator for the detection and discrimination of cellular events by sc-ICP-MS for quadrupole (Q) mass spectrometers. Additionally, applying this analytical method to cultured MIO-M1 cells under hypoxic and to normoxic conditions showcased the ability of sc-ICP-QMS to study hypoxia-induced changes in the cell-to-cell distributions of HIF-1α and VEGF levels.

单细胞电感耦合等离子体质谱法（sc-ICP-MS）可对单个细胞内的元素和生物大分子进行鉴定和定量。利用基于金属纳米簇（MNCs）的免疫探针，sc-ICP-MS 可以分析目标蛋白质，从而有助于检测每个细胞中的低量蛋白质。然而，要全面实施 sc-ICP-MS，还需要一种有效的策略来加强对细胞事件的检测。在这项工作中，研究了如何测量内源元素和两种外部标记（钌红和 Rh-DNA 中间体）以进行细胞检测。作为概念验证，研究人员对单个人类 Müller 胶质细胞（MIO-M1）中的两种蛋白质（缺氧诱导因子-1α 和血管内皮生长因子；分别为 HIF-1α 和 VEGF）进行了连续测定。针对 HIF-1α 和 VEGF 的特异性抗体分别用 AuNCs 和 IrNCs 标记。结果表明，在四极杆（Q）质谱仪的 sc-ICP-MS 中使用 Rh-DNA 中间体检测和区分细胞事件具有优势。此外，将这种分析方法应用于缺氧和正常缺氧条件下培养的 MIO-M1 细胞，展示了 sc-ICP-QMS 研究缺氧诱导的细胞间 HIF-1α 和 VEGF 水平分布变化的能力。

{"title":"Determination of proteins in hypoxic Müller glia cells by single-cell ICP-MS: The use of a Rh-DNA intercalator for enhanced cell detection","authors":"Alicia Villa-Vázquez , Paula Menero-Valdés , Lydia Álvarez , Héctor González-Iglesias , Beatriz Fernández , Rosario Pereiro","doi":"10.1016/j.microc.2024.111745","DOIUrl":"10.1016/j.microc.2024.111745","url":null,"abstract":"<div><div>Single cell − inductively coupled plasma-mass spectrometry (sc-ICP-MS) enables the identification and quantification of elements and biomolecules within individual cells. Target proteins can be analyzed by sc-ICP-MS using metal nanoclusters (MNCs)-based immunoprobes, facilitating the detection of low amounts of proteins in each cell. However, for the full implementation of sc-ICP-MS, an effective strategy to enhance the detection of cellular events is required. In this work, the measurement of endogenous elements and two external tags (ruthenium red and Rh-DNA intercalator) was investigated for cell detection. As a proof of concept, the sequential determination of two proteins (hypoxia inducible factor-1α and vascular endothelial growth factor; HIF-1α and VEGF, respectively) in individual human Müller glia cells (MIO-M1) was pursued. Specific antibodies against HIF-1α and VEGF were labelled with AuNCs and IrNCs, respectively. The results demonstrated the advantages of using Rh-DNA intercalator for the detection and discrimination of cellular events by sc-ICP-MS for quadrupole (Q) mass spectrometers. Additionally, applying this analytical method to cultured MIO-M1 cells under hypoxic and to normoxic conditions showcased the ability of sc-ICP-QMS to study hypoxia-induced changes in the cell-to-cell distributions of HIF-1α and VEGF levels.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"207 ","pages":"Article 111745"},"PeriodicalIF":4.9,"publicationDate":"2024-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142318507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A highly sensitive sandwich-type electrochemical sensor for detection glypican-3 based on H-rGO-CMC@Pt NPs and aptamers 基于 H-rGO-CMC@Pt NPs 和适配体的高灵敏度夹心型电化学传感器，用于检测 glypican-3

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2024-09-22 DOI: 10.1016/j.microc.2024.111747

Xiaohong Tan , Wenzhan Li , Wei He , Tingting Yu , Bingbing Wan , Yong Huang , Jintao Liang , Guiyin Li

Glypican-3 (GPC3), which is a surface heparan sulfate proteoglycan, has been widely known the ideal biomarker for diagnosis and treatment of hepatocellular carcinoma (HCC). Accurate and sensitive detection of serum GPC3 level is an important and challenging task. In this paper, a sandwich electrochemical aptasensor was designed to specifically detect serum GPC3 level through aptamer-target-aptamer recognition. A GPC3 aptamer (GPC3_Apt) immobilized on the surface of gold nanoparticles@reduced graphene oxide modified screen-printed electrode (Au NPs@rGO/SPCE) was used as the capture probe. Hemin-reduced graphene oxide-carboxymethyl chitosan@platinum nanoparticles (H-rGO-CMC@Pt NPs), with good mimicking peroxidases activity, were labeled with amination GPC3 aptamer, which was employed as the signal probe. Once the target GPC3 was anchored on the surface of GPC3_Apt/Au NPs@rGO/SPCE, the H-rGO-CMC@Pt NPs-GPC3_Apt was further specifically bound to the GPC3, forming the aptamer-target-aptamer sandwich structure, which can catalyze the oxidation of hydroquinone (HQ) to benzoquinone (BQ) and lead the oxidation current of HQ recorded by DPV to changing. The linear relationship between the current signal of oxidation of HQ and GPC3 concentration range of 0.0001–3.0 µg/mL and 3.0–60.0 µg/mL, with a low detection limit (LOD) of 0.0685 ng/mL. Additionally, the electrochemical aptasensor was successfully applied for the detection of GPC3 in human serum samples with the recovery of 99.95–104.06 % and relative standard deviation (RSD) of 1.31–5.22 %. Thus, the sandwich aptasensor could provide a new strategy for detection of serum GPC3 level and improve the early diagnosis rate of HCC.

Glypican-3（GPC3）是一种表面硫酸肝素蛋白多糖，已被广泛认为是诊断和治疗肝细胞癌（HCC）的理想生物标志物。准确而灵敏地检测血清 GPC3 水平是一项重要而具有挑战性的任务。本文设计了一种夹心电化学适配体传感器，通过适配体-目标-适配体的识别来特异性检测血清 GPC3 水平。GPC3 aptamer（GPC3Apt）被固定在金纳米粒子@还原氧化石墨烯修饰的丝网印刷电极（Au NPs@rGO/SPCE）表面作为捕获探针。血红素还原氧化石墨烯-羧甲基壳聚糖@铂纳米粒子（H-rGO-CMC@Pt NPs）具有良好的模拟过氧化物酶活性，其上标记了氨基化 GPC3 合酶，作为信号探针。目标 GPC3 被锚定在 GPC3Apt/Au NPs@rGO/SPCE 表面后，H-rGO-CMC@Pt NPs-GPC3Apt 进一步与 GPC3 特异性结合，形成aptamer-目标-aptamer 的三明治结构，从而催化对苯二酚（HQ）氧化成苯醌（BQ），并导致 DPV 记录的 HQ 氧化电流发生变化。HQ 氧化电流信号与 GPC3 浓度的线性关系范围分别为 0.0001-3.0 µg/mL 和 3.0-60.0 µg/mL，低检测限为 0.0685 ng/mL。此外，该电化学适配传感器还成功用于检测人血清样品中的 GPC3，回收率为 99.95-104.06%，相对标准偏差（RSD）为 1.31-5.22%。因此，夹心式灵敏传感器可为血清 GPC3 水平的检测提供一种新策略，并提高 HCC 的早期诊断率。

{"title":"A highly sensitive sandwich-type electrochemical sensor for detection glypican-3 based on H-rGO-CMC@Pt NPs and aptamers","authors":"Xiaohong Tan , Wenzhan Li , Wei He , Tingting Yu , Bingbing Wan , Yong Huang , Jintao Liang , Guiyin Li","doi":"10.1016/j.microc.2024.111747","DOIUrl":"10.1016/j.microc.2024.111747","url":null,"abstract":"<div><div>Glypican-3 (GPC3), which is a surface heparan sulfate proteoglycan, has been widely known the ideal biomarker for diagnosis and treatment of hepatocellular carcinoma (HCC). Accurate and sensitive detection of serum GPC3 level is an important and challenging task. In this paper, a sandwich electrochemical aptasensor was designed to specifically detect serum GPC3 level through aptamer-target-aptamer recognition. A GPC3 aptamer (GPC3<sub>Apt</sub>) immobilized on the surface of gold nanoparticles@reduced graphene oxide modified screen-printed electrode (Au NPs@rGO/SPCE) was used as the capture probe. Hemin-reduced graphene oxide-carboxymethyl chitosan@platinum nanoparticles (H-rGO-CMC@Pt NPs), with good mimicking peroxidases activity, were labeled with amination GPC3 aptamer, which was employed as the signal probe. Once the target GPC3 was anchored on the surface of GPC3<sub>Apt</sub>/Au NPs@rGO/SPCE, the H-rGO-CMC@Pt NPs-GPC3<sub>Apt</sub> was further specifically bound to the GPC3, forming the aptamer-target-aptamer sandwich structure, which can catalyze the oxidation of hydroquinone (HQ) to benzoquinone (BQ) and lead the oxidation current of HQ recorded by DPV to changing. The linear relationship between the current signal of oxidation of HQ and GPC3 concentration range of 0.0001–3.0 µg/mL and 3.0–60.0 µg/mL, with a low detection limit (LOD) of 0.0685 ng/mL. Additionally, the electrochemical aptasensor was successfully applied for the detection of GPC3 in human serum samples with the recovery of 99.95–104.06 % and relative standard deviation (RSD) of 1.31–5.22 %. Thus, the sandwich aptasensor could provide a new strategy for detection of serum GPC3 level and improve the early diagnosis rate of HCC.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"207 ","pages":"Article 111747"},"PeriodicalIF":4.9,"publicationDate":"2024-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142314998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nanozymes in environmental remediation: A bibliometric and comprehensive review of their oxidoreductase-mimicking capabilities 环境修复中的纳米酶：关于氧化还原酶模拟能力的文献计量和综合评述

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2024-09-21 DOI: 10.1016/j.microc.2024.111748

Vasundra Nagendran , Louella Concepta Goveas , Ramesh Vinayagam , Thivaharan Varadavenkatesan , Raja Selvaraj

Nanozymes mimic enzyme-like properties and possess unique characteristics inherent to nanomaterials, combining catalytic functionality with distinct nanostructure features. This class of emerging materials has garnered significant attention for their potential applications in environmental remediation. A bibliometric analysis was conducted to map research trends and identify key areas of focus in this rapidly evolving field. This review comprehensively examines the types and catalytic mechanisms of oxidoreductase nanozymes, and their diverse environmental applications with specific attention to detection and degradation of various pollutants such as dyes, pesticides, antibiotics and toxic ions. The intricate mechanisms of oxidoreductases mimicked by nanozymes and their roles in catalysing reactions for environmental remediation are discussed. Furthermore, their versatility and efficacy in addressing environmental challenges are elaborated with recent literature. Through a thorough analysis of recent advancements and case studies, this review provides valuable insights into the potential of nanozymes as efficient tools for sustainable environmental management.

纳米酶模仿酶的特性，具有纳米材料固有的独特特征，将催化功能与独特的纳米结构特征相结合。这一类新兴材料因其在环境修复方面的潜在应用而备受关注。我们进行了文献计量分析，以绘制研究趋势图，并确定这一快速发展领域的重点领域。本综述全面研究了氧化还原酶纳米分子的类型和催化机制，以及它们在环境中的各种应用，特别关注各种污染物（如染料、杀虫剂、抗生素和有毒离子）的检测和降解。讨论了纳米酶模仿氧化还原酶的复杂机制及其在环境修复催化反应中的作用。此外，还结合最新文献阐述了它们在应对环境挑战方面的多功能性和功效。通过对最新进展和案例研究的透彻分析，本综述为了解纳米微生物作为可持续环境管理有效工具的潜力提供了宝贵的见解。

{"title":"Nanozymes in environmental remediation: A bibliometric and comprehensive review of their oxidoreductase-mimicking capabilities","authors":"Vasundra Nagendran , Louella Concepta Goveas , Ramesh Vinayagam , Thivaharan Varadavenkatesan , Raja Selvaraj","doi":"10.1016/j.microc.2024.111748","DOIUrl":"10.1016/j.microc.2024.111748","url":null,"abstract":"<div><div>Nanozymes mimic enzyme-like properties and possess unique characteristics inherent to nanomaterials, combining catalytic functionality with distinct nanostructure features. This class of emerging materials has garnered significant attention for their potential applications in environmental remediation. A bibliometric analysis was conducted to map research trends and identify key areas of focus in this rapidly evolving field. This review comprehensively examines the types and catalytic mechanisms of oxidoreductase nanozymes, and their diverse environmental applications with specific attention to detection and degradation of various pollutants such as dyes, pesticides, antibiotics and toxic ions. The intricate mechanisms of oxidoreductases mimicked by nanozymes and their roles in catalysing reactions for environmental remediation are discussed. Furthermore, their versatility and efficacy in addressing environmental challenges are elaborated with recent literature. Through a thorough analysis of recent advancements and case studies, this review provides valuable insights into the potential of nanozymes as efficient tools for sustainable environmental management.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"207 ","pages":"Article 111748"},"PeriodicalIF":4.9,"publicationDate":"2024-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142322198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

3D-printing and MATLAB assisted ratiometric-sensing platform for visual on-site sensing of tetracycline using color conversion strategy 三维打印和 MATLAB 辅助比率测量传感平台，利用色彩转换策略实现四环素的视觉现场传感

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2024-09-21 DOI: 10.1016/j.microc.2024.111730

Ruimei Zhang , Dong Zeng , Yuxin Guo , Jiawei Huang , Ruizhi Wen , Hongchang Li

In this research, a ratiometric-sensing platform (ZIF-8/CDs) that combines non-fluorescence metal–organic frameworks (ZIF-8) with blue-green fluorescent carbon dots (CDs) has been developed for the determination of tetracycline (TC) with high sensitivity and selectivity in real samples. For this purpose, ZIF-8 with polyhedral structure was synthesized, which could form aggregation-induced emission (AIE) effect through electrostatic, hydrogen bonding, π-π stacking, and coordination interactions in the presence of TC, leading to enhanced intensity with a bright yellow fluorescence at 516 nm, acting as a response signal. However, the fluorescence intensity of blue-green fluorescent CDs maintained practically constant at 466 nm, serving as a reference signal. Furthermore, we integrate MATLAB algorithm and 3D-printing technology with the ZIF-8/CDs complex into a ratiometric-sensing platform for semi-quantitative detection of TC, in which 3D-printing technology is responsible for engineering an integrated dark chamber to achieve the fluorescence images of real samples, whereas MATLAB with the image processing functions and data processing capabilities utilizes to develop a mini program for calculating the RGB values of the obtained images. Consequently, the ratiometric-sensing platform in a simple, rapid, and economic way without any complicated process and expensive instruments could be used for visual/onsite detection of TC in real samples.

本研究开发了一种将非荧光金属有机框架（ZIF-8）与蓝绿荧光碳点（CD）相结合的比率传感平台（ZIF-8/CDs），用于实际样品中四环素（TC）的高灵敏度和高选择性测定。为此，合成了具有多面体结构的 ZIF-8，在 TC 存在的情况下，ZIF-8 可通过静电、氢键、π-π 堆积和配位相互作用形成聚集诱导发射（AIE）效应，从而在 516 纳米波长处增强发出亮黄色荧光，作为响应信号。然而，蓝绿荧光 CD 在 466 纳米波长处的荧光强度几乎保持不变，可作为参考信号。此外，我们还将 MATLAB 算法和三维打印技术与 ZIF-8/CDs 复合物集成到一个半定量检测 TC 的比率感应平台中，其中三维打印技术负责设计一个集成暗室来实现真实样品的荧光图像，而具有图像处理功能和数据处理能力的 MATLAB 则用于开发一个小型程序来计算所获得图像的 RGB 值。因此，无需任何复杂的过程和昂贵的仪器，就能以简单、快速和经济的方式利用比率测量传感平台对真实样品中的 TC 进行视觉/现场检测。

{"title":"3D-printing and MATLAB assisted ratiometric-sensing platform for visual on-site sensing of tetracycline using color conversion strategy","authors":"Ruimei Zhang , Dong Zeng , Yuxin Guo , Jiawei Huang , Ruizhi Wen , Hongchang Li","doi":"10.1016/j.microc.2024.111730","DOIUrl":"10.1016/j.microc.2024.111730","url":null,"abstract":"<div><div>In this research, a ratiometric-sensing platform (ZIF-8/CDs) that combines non-fluorescence metal–organic frameworks (ZIF-8) with blue-green fluorescent carbon dots (CDs) has been developed for the determination of tetracycline (TC) with high sensitivity and selectivity in real samples. For this purpose, ZIF-8 with polyhedral structure was synthesized, which could form aggregation-induced emission (AIE) effect through electrostatic, hydrogen bonding, π-π stacking, and coordination interactions in the presence of TC, leading to enhanced intensity with a bright yellow fluorescence at 516 nm, acting as a response signal. However, the fluorescence intensity of blue-green fluorescent CDs maintained practically constant at 466 nm, serving as a reference signal. Furthermore, we integrate MATLAB algorithm and 3D-printing technology with the ZIF-8/CDs complex into a ratiometric-sensing platform for semi-quantitative detection of TC, in which 3D-printing technology is responsible for engineering an integrated dark chamber to achieve the fluorescence images of real samples, whereas MATLAB with the image processing functions and data processing capabilities utilizes to develop a mini program for calculating the RGB values of the obtained images. Consequently, the ratiometric-sensing platform in a simple, rapid, and economic way without any complicated process and expensive instruments could be used for visual/onsite detection of TC in real samples.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"207 ","pages":"Article 111730"},"PeriodicalIF":4.9,"publicationDate":"2024-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142317845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A cutting edge potentiometric determination of baricitinib in synthetic wastewater and in tablet dosage form using modified carbon paste ion-selective membrane 利用改性碳浆离子选择膜测定合成废水和片剂中巴利替尼的尖端电位测定法

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2024-09-21 DOI: 10.1016/j.microc.2024.111727

Heba T. Elbalkiny , Mona S. Elshahed , Dalia Mohamed , Azza A. Ashour , Rasha Th. El-Eryan

After the recent hit of COVID-19, many drugs were successively administered to save patients’ lives and those drugs reached aquatic sources, one of those drugs was Baricitinib. Driven by the current situation, a potentiometric technique using an ion-selective membrane (ISM) recipe drop cast on a modified carbon paste electrode was optimized for the superior determination of Baricitinib (BAR). The molecular docking was customized to optimize the proper ionophores incorporated in the ISM, revealing that beta-cyclodextrin (β-CD) is the most compatible ionophore with the studied drug. A multivariate optimization experimental design was employed to optimize the best experimental condition for the analytical method. To this end; dual nanoparticles (multi-walled carbon nanotubes and copper oxide nanoparticles) were incorporated in the carbon paste electrode, and the ISM recipe was enriched with β-CD and cation-exchanger (phosphotungstic acid) in a polyvinylchloride matrix plasticized with dibutyl phthalate. A Nernstian slope equal to 19.97 mV/decade with a linearity range of 7.99 × 10⁻⁷–1.00 × 10⁻³ M was obtained. The validated sensor exhibited good recovery when used to determine the studied drug in synthetic wastewater and tablet dosage form. The greenness of the method was evaluated using the Complementary Green Analytical Procedure Index and Blue Applicability Grade Index.

最近，COVID-19 一炮打响后，许多药物被陆续用于挽救病人的生命，这些药物流入了水产来源，巴利昔替尼就是其中之一。在这一现状的推动下，为了更好地测定巴利替尼（BAR），我们优化了离子选择膜（ISM）配方，并将其滴在改性碳浆电极上。通过分子对接优化了 ISM 中适当的离子载体，发现β-环糊精（β-CD）是与所研究药物最兼容的离子载体。为了优化分析方法的最佳实验条件，采用了多元优化实验设计。为此，在碳浆电极中加入了双纳米粒子（多壁碳纳米管和纳米氧化铜），并在用邻苯二甲酸二丁酯塑化的聚氯乙烯基质中添加了 β-CD 和阳离子交换剂（磷钨酸）的 ISM 配方。得到的 Nernstian 斜率等于 19.97 mV/decade，线性范围为 7.99 × 10-7-1.00 × 10-3 M。在测定合成废水和片剂中的药物时，经验证的传感器具有良好的回收率。该方法的绿色性采用补充绿色分析程序指数和蓝色适用等级指数进行评估。

{"title":"A cutting edge potentiometric determination of baricitinib in synthetic wastewater and in tablet dosage form using modified carbon paste ion-selective membrane","authors":"Heba T. Elbalkiny , Mona S. Elshahed , Dalia Mohamed , Azza A. Ashour , Rasha Th. El-Eryan","doi":"10.1016/j.microc.2024.111727","DOIUrl":"10.1016/j.microc.2024.111727","url":null,"abstract":"<div><div>After the recent hit of COVID-19, many drugs were successively administered to save patients’ lives and those drugs reached aquatic sources, one of those drugs was Baricitinib. Driven by the current situation, a potentiometric technique using an ion-selective membrane (ISM) recipe drop cast on a modified carbon paste electrode was optimized for the superior determination of Baricitinib (BAR). The molecular docking was customized to optimize the proper ionophores incorporated in the ISM, revealing that beta-cyclodextrin (β-CD) is the most compatible ionophore with the studied drug. A multivariate optimization experimental design was employed to optimize the best experimental condition for the analytical method. To this end; dual nanoparticles (multi-walled carbon nanotubes and copper oxide nanoparticles) were incorporated in the carbon paste electrode, and the ISM recipe was enriched with β-CD and cation-exchanger (phosphotungstic acid) in a polyvinylchloride matrix plasticized with dibutyl phthalate. A Nernstian slope equal to 19.97 mV/decade with a linearity range of 7.99 × 10<sup>−7</sup>–1.00 × 10<sup>−3</sup> M was obtained. The validated sensor exhibited good recovery when used to determine the studied drug in synthetic wastewater and tablet dosage form. The greenness of the method was evaluated using the Complementary Green Analytical Procedure Index and Blue Applicability Grade Index.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"207 ","pages":"Article 111727"},"PeriodicalIF":4.9,"publicationDate":"2024-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142357861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fluorescent sensing platform based on two-dimensional layered double hydroxides and exonuclease I for DNA and microRNA detection 基于二维层状双氢氧化物和外切酶 I 的用于 DNA 和 microRNA 检测的荧光传感平台

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2024-09-21 DOI: 10.1016/j.microc.2024.111740

Yuanyi Wu , Yan Yang , Shihao Yang , Yang Ju , Yang Jiang , Yumei Xiao , Jialin He , Tian Liu , Leyao Tang , Kaiyong Wang , Yi Yang

Interfacial and interlayer interactions between layered double hydroxides (LDHs) and nucleic acids offer LDHs great potential to absorb and detect nucleic acids. Herein, we demonstrate a simple, rapid, and versatile fluorescent sensing platform based on two-dimensional LDHs and exonuclease I (Exo I) for the selective analysis of DNA and microRNA. ZnNiAl-LDHs were utilized as quenchers for FAM labelled on single-stranded DNA (ssDNA) probes via dynamic quenching with high fluorescence quenching ability. The quenching mechanism was explored by means of spectral analysis, characterization experiments, DNA adsorption and desorption studies, zeta potential analysis, and molecular dynamics simulations. The driving forces for the adsorption of LDHs toward fluorophore-labelled nucleic acids were confirmed to be electrostatic interaction, van der Waals force, and anion exchange for the first time. In order to distinguish the formed target/probe duplexes from FAM-ssDNA probes, Exo I was introduced to specifically hydrolyze FAM-ssDNA probes. The fabricated fluorescent sensing platform for nucleic acids detection shows a low detection limit of 0.08 nM. Finally, this sensor was effectively utilized for the detection of DNA and microRNA in actual biological and environmental samples, achieving recoveries ranging from 96.4 % to 105 %, indicating high accuracy. This work proposed a promising sensing platform for sequence-specific nucleic acids detection, and provides a new perspective on two-dimensional LDHs as structural supports and biomolecular reservoirs for biomedical applications.

层状双氢氧化物（LDHs）与核酸之间的界面和层间相互作用为 LDHs 吸收和检测核酸提供了巨大的潜力。在此，我们展示了一种基于二维 LDHs 和外切酶 I（Exo I）的简单、快速、多功能荧光传感平台，用于选择性分析 DNA 和 microRNA。ZnNiAl-LDHs 被用作单链 DNA（ssDNA）探针上 FAM 标记的淬灭剂，通过高荧光淬灭能力进行动态淬灭。通过光谱分析、表征实验、DNA 吸附和解吸研究、zeta 电位分析和分子动力学模拟，对淬灭机制进行了探索。首次证实了 LDHs 对荧光团标记核酸的吸附驱动力是静电作用、范德华力和阴离子交换。为了从 FAM-ssDNA 探针中区分已形成的目标/探针双链体，引入了 Exo I 来特异性水解 FAM-ssDNA 探针。所制作的核酸检测荧光传感平台的检测限低至 0.08 nM。最后，该传感器被有效地用于检测实际生物和环境样品中的 DNA 和 microRNA，回收率从 96.4 % 到 105 % 不等，表明其准确性很高。这项工作为序列特异性核酸检测提出了一个前景广阔的传感平台，并为二维 LDHs 作为生物医学应用的结构支撑和生物分子库提供了一个新的视角。

{"title":"Fluorescent sensing platform based on two-dimensional layered double hydroxides and exonuclease I for DNA and microRNA detection","authors":"Yuanyi Wu , Yan Yang , Shihao Yang , Yang Ju , Yang Jiang , Yumei Xiao , Jialin He , Tian Liu , Leyao Tang , Kaiyong Wang , Yi Yang","doi":"10.1016/j.microc.2024.111740","DOIUrl":"10.1016/j.microc.2024.111740","url":null,"abstract":"<div><div>Interfacial and interlayer interactions between layered double hydroxides (LDHs) and nucleic acids offer LDHs great potential to absorb and detect nucleic acids. Herein, we demonstrate a simple, rapid, and versatile fluorescent sensing platform based on two-dimensional LDHs and exonuclease I (Exo I) for the selective analysis of DNA and microRNA. ZnNiAl-LDHs were utilized as quenchers for FAM labelled on single-stranded DNA (ssDNA) probes via dynamic quenching with high fluorescence quenching ability. The quenching mechanism was explored by means of spectral analysis, characterization experiments, DNA adsorption and desorption studies, zeta potential analysis, and molecular dynamics simulations. The driving forces for the adsorption of LDHs toward fluorophore-labelled nucleic acids were confirmed to be electrostatic interaction, van der Waals force, and anion exchange for the first time. In order to distinguish the formed target/probe duplexes from FAM-ssDNA probes, Exo I was introduced to specifically hydrolyze FAM-ssDNA probes. The fabricated fluorescent sensing platform for nucleic acids detection shows a low detection limit of 0.08 nM. Finally, this sensor was effectively utilized for the detection of DNA and microRNA in actual biological and environmental samples, achieving recoveries ranging from 96.4 % to 105 %, indicating high accuracy. This work proposed a promising sensing platform for sequence-specific nucleic acids detection, and provides a new perspective on two-dimensional LDHs as structural supports and biomolecular reservoirs for biomedical applications.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"207 ","pages":"Article 111740"},"PeriodicalIF":4.9,"publicationDate":"2024-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142317844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Potential of high-throughput FIA-MS/MS and LC-MS/MS polyphenolic profiling to assess tea authenticity. Application to tea adulterations with chicory 高通量 FIA-MS/MS 和 LC-MS/MS 多酚分析评估茶叶真伪的潜力。在掺入菊苣的茶叶中的应用

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2024-09-21 DOI: 10.1016/j.microc.2024.111723

Thom Romers , Sònia Sentellas , Javier Saurina , Oscar Núñez

Tea can be found among the beverages more susceptible to fraudulent practices because of its high worldwide consumption and the increases on prices for some specific varieties due to climate change and geopolitical instability. Tea adulteration with other plants, such as chicory, is a common practice to gain an illicit profit. Polyphenols are abundant bioactive substances in tea, determining its quality and health function. In addition, they can be employed as markers to address authentication issues. The present contribution assesses the potential of polyphenolic profiling by high-throughput FIA-MS/MS and LC-MS/MS methodologies for tea authenticity. One hundred tea samples belonging to different varieties (green, black, red, oolong, and white teas) and 20 chicory samples were analyzed with both methodologies after a simple brewing process to profile fifty-five polyphenols belonging to different families. The resulting chemical descriptors were used to address tea classification and authentication by partial least-squares-discriminant analysis (PLS-DA). An excellent classification performance by PLS-DA was accomplished, with sensitivity and specificity values for FIA-MS/MS higher than 90% and 88.9%, respectively, and for LC-MS/MS higher than 85% and 86%, respectively. Good accuracy was also attained, with calibration errors below 10.5 and 14.5% for FIA-MS/MS and LC-MS/MS, respectively. Overall, FIA-MS/MS showed a better performance than LC-MS/MS, with the additional advantage of shorter analysis time as no chromatographic separation was required. The capability of phenolics to quantify tea adulterations with chicory was also assessed by partial least squares (PLS) regression, with prediction errors below 10.9 and 14.8% for FIA-MS/MS and LC-MS/MS, respectively, in the determination of adulterant levels. Thus, both methodologies demonstrated to be feasible for assessing tea authentication issues.

茶叶是最容易受到欺诈行为影响的饮料之一，因为它在全世界的消费量很高，而且由于气候变化和地缘政治不稳定，一些特定品种的茶叶价格上涨。在茶叶中掺杂菊苣等其他植物是获取非法利润的常见做法。茶多酚是茶叶中丰富的生物活性物质，决定着茶叶的品质和保健功能。此外，茶多酚还可用作解决鉴定问题的标记。本文通过高通量 FIA-MS/MS 和 LC-MS/MS 方法评估了茶叶真伪的多酚分析潜力。在经过简单的冲泡过程后，采用这两种方法分析了 100 个不同品种的茶叶样品（绿茶、红茶、红茶、乌龙茶和白茶）和 20 个菊苣样品，以确定属于不同系列的 55 种茶多酚。得出的化学描述符被用于通过偏最小二乘判别分析（PLS-DA）对茶叶进行分类和鉴定。PLS-DA 的分类效果极佳，FIA-MS/MS 的灵敏度和特异度分别高于 90% 和 88.9%，LC-MS/MS 的灵敏度和特异度分别高于 85% 和 86%。FIA-MS/MS 和 LC-MS/MS 的校准误差分别低于 10.5%和 14.5%，准确度也很高。总体而言，FIA-MS/MS 的性能优于 LC-MS/MS，而且由于无需色谱分离，分析时间更短。通过偏最小二乘法（PLS）回归评估了酚类物质定量掺杂菊苣的茶叶的能力，在确定掺杂物水平时，FIA-MS/MS 和 LC-MS/MS 的预测误差分别低于 10.9% 和 14.8%。因此，这两种方法在评估茶叶鉴别问题上都是可行的。

{"title":"Potential of high-throughput FIA-MS/MS and LC-MS/MS polyphenolic profiling to assess tea authenticity. Application to tea adulterations with chicory","authors":"Thom Romers , Sònia Sentellas , Javier Saurina , Oscar Núñez","doi":"10.1016/j.microc.2024.111723","DOIUrl":"10.1016/j.microc.2024.111723","url":null,"abstract":"<div><div>Tea can be found among the beverages more susceptible to fraudulent practices because of its high worldwide consumption and the increases on prices for some specific varieties due to climate change and geopolitical instability. Tea adulteration with other plants, such as chicory, is a common practice to gain an illicit profit. Polyphenols are abundant bioactive substances in tea, determining its quality and health function. In addition, they can be employed as markers to address authentication issues. The present contribution assesses the potential of polyphenolic profiling by high-throughput FIA-MS/MS and LC-MS/MS methodologies for tea authenticity. One hundred tea samples belonging to different varieties (green, black, red, oolong, and white teas) and 20 chicory samples were analyzed with both methodologies after a simple brewing process to profile fifty-five polyphenols belonging to different families. The resulting chemical descriptors were used to address tea classification and authentication by partial least-squares-discriminant analysis (PLS-DA). An excellent classification performance by PLS-DA was accomplished, with sensitivity and specificity values for FIA-MS/MS higher than 90% and 88.9%, respectively, and for LC-MS/MS higher than 85% and 86%, respectively. Good accuracy was also attained, with calibration errors below 10.5 and 14.5% for FIA-MS/MS and LC-MS/MS, respectively. Overall, FIA-MS/MS showed a better performance than LC-MS/MS, with the additional advantage of shorter analysis time as no chromatographic separation was required. The capability of phenolics to quantify tea adulterations with chicory was also assessed by partial least squares (PLS) regression, with prediction errors below 10.9 and 14.8% for FIA-MS/MS and LC-MS/MS, respectively, in the determination of adulterant levels. Thus, both methodologies demonstrated to be feasible for assessing tea authentication issues.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"207 ","pages":"Article 111723"},"PeriodicalIF":4.9,"publicationDate":"2024-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0026265X24018356/pdfft?md5=7fec1c6224a25afd2c9751d5ce03ded3&pid=1-s2.0-S0026265X24018356-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142315094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Two-pot synthesis of polypyrrole‐coated rubber foam for the extraction of carbendazim residue in orange juice 用于提取橙汁中多菌灵残留物的聚吡咯涂层橡胶泡沫的双锅合成法

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2024-09-21 DOI: 10.1016/j.microc.2024.111712

Teerayanee Chaipet , Suwatchanee Maneeratanachot , Nussana Lehman , Ekwipoo Kalkornsurapranee , Wing Cheung Mak , Proespichaya Kanatharana , Panote Thavarungkul , Chongdee Thammakhet-Buranachai

Natural rubber foam was coated with polypyrrole (PPy) via a rapid two-pot synthesis. This PPy-coated rubber foam was used as sorbent for the solid-phase extraction of carbendazim in fresh orange juice. The extracted carbendazim was quantified by high performance liquid chromatography with diode array detection (HPLC-DAD). Under the optimal conditions, the method exhibited linearity in the range of 25–1000 µg kg⁻¹ with a limit of detection (LOD) of 24.1 µg kg⁻¹ and a limit of quantification (LOQ) of 80.5 µg kg⁻¹. Good reproducibility (RSD < 2.5 %, n = 6) and good reusability (RSD < 3.8 %, n = 10) were achieved. The sorbent was applied to extract carbendazim in fresh orange juice. Accuracy was excellent and recoveries were attained from 81.6 % to 104.8 %. The found carbendazim in real samples ranged from 40.0 µg kg⁻¹ to 101.1 µg kg⁻¹. The developed sorbent can be produced in large quantities at a reasonable cost and utilized as an environmentally friendly sorbent for the extraction of pesticides and other trace organic substances.

通过快速双锅合成法在天然橡胶泡沫上涂覆聚吡咯（PPy）。这种涂有聚吡咯的橡胶泡沫被用作吸附剂，用于固相萃取新鲜橙汁中的多菌灵。提取的多菌灵采用高效液相色谱-二极管阵列检测法（HPLC-DAD）进行定量。在最佳条件下，该方法的线性范围为 25-1000 µg kg-1，检出限（LOD）为 24.1 µg kg-1，定量限（LOQ）为 80.5 µg kg-1。重现性良好（RSD < 2.5 %，n = 6），重复使用性良好（RSD < 3.8 %，n = 10）。该吸附剂用于提取新鲜橙汁中的多菌灵。准确度极高，回收率在 81.6 % 到 104.8 % 之间。实际样品中的多菌灵含量为 40.0 µg kg-1 至 101.1 µg kg-1。所开发的吸附剂可以以合理的成本大量生产，并可作为一种环保型吸附剂用于提取农药和其他痕量有机物质。

{"title":"Two-pot synthesis of polypyrrole‐coated rubber foam for the extraction of carbendazim residue in orange juice","authors":"Teerayanee Chaipet , Suwatchanee Maneeratanachot , Nussana Lehman , Ekwipoo Kalkornsurapranee , Wing Cheung Mak , Proespichaya Kanatharana , Panote Thavarungkul , Chongdee Thammakhet-Buranachai","doi":"10.1016/j.microc.2024.111712","DOIUrl":"10.1016/j.microc.2024.111712","url":null,"abstract":"<div><div>Natural rubber foam was coated with polypyrrole (PPy) via a rapid two-pot synthesis. This PPy-coated rubber foam was used as sorbent for the solid-phase extraction of carbendazim in fresh orange juice. The extracted carbendazim was quantified by high performance liquid chromatography with diode array detection (HPLC-DAD). Under the optimal conditions, the method exhibited linearity in the range of 25–1000 µg kg<sup>−1</sup> with a limit of detection (LOD) of 24.1 µg kg<sup>−1</sup> and a limit of quantification (LOQ) of 80.5 µg kg<sup>−1</sup>. Good reproducibility (RSD < 2.5 %, n = 6) and good reusability (RSD < 3.8 %, n = 10) were achieved. The sorbent was applied to extract carbendazim in fresh orange juice. Accuracy was excellent and recoveries were attained from 81.6 % to 104.8 %. The found carbendazim in real samples ranged from 40.0 µg kg<sup>−1</sup> to 101.1 µg kg<sup>−1</sup>. The developed sorbent can be produced in large quantities at a reasonable cost and utilized as an environmentally friendly sorbent for the extraction of pesticides and other trace organic substances.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"207 ","pages":"Article 111712"},"PeriodicalIF":4.9,"publicationDate":"2024-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142311085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effect of energy modality on tissue identification from surgical smoke by differential ion mobility spectrometry 能量模式对差分离子迁移谱法识别手术烟雾中组织的影响

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2024-09-21 DOI: 10.1016/j.microc.2024.111733

Patrik Sioris , Meri Mäkelä , Anton Kontunen , Markus Karjalainen , Antti Vehkaoja , Niku Oksala , Antti Roine

Surgical smoke analysis offers a way to provide assisting information to the surgeon intraoperatively, which can be potentially used to assess cancer tumor margins in surgical oncology and alert the operator of accidental organ injuries caused by electrosurgical (ES) instruments. Surgical smoke content is affected by the energy instrument it is produced by. Classification of surgical smoke by differential ion mobility spectrometry (DMS) was evaluated with 6 porcine tissue types and 5 energy instruments. Instruments consisted of mono- and bipolar instruments, ultrasonic shears and a –blade. Machine learning was used to classify tissues by training binary classifier linear discriminant analysis (LDA) to distinguish a marked tissue class from the rest. The greatest binary classification accuracies were obtained with the monopolar instruments and the lowest with the bipolar instrument, 93.5 % and 77.5 % respectively. The analysis of surgical smoke with DMS is possible with a variety of energy instruments, however with varying performance. This implies that DMS based tissue identification is generalizable across different surgical instruments and surgical specialties.

手术烟雾分析提供了一种在术中向外科医生提供辅助信息的方法，可用于评估肿瘤外科手术中的肿瘤边缘，并提醒操作者注意电外科（ES）器械造成的意外器官损伤。手术烟雾的含量受其产生的能量仪器的影响。通过差分离子迁移谱法（DMS）对 6 种猪组织类型和 5 种能量仪器进行了手术烟雾分类评估。仪器包括单极和双极仪器、超声波剪和刀片。通过训练二元分类器线性判别分析 (LDA)，使用机器学习对组织进行分类，以区分有标记的组织类别和其他类别。单极器械的二元分类准确率最高，双极器械最低，分别为 93.5 % 和 77.5 %。使用 DMS 分析手术烟雾可以使用多种能量仪器，但性能各不相同。这意味着基于 DMS 的组织识别可适用于不同的手术器械和手术专业。

{"title":"The effect of energy modality on tissue identification from surgical smoke by differential ion mobility spectrometry","authors":"Patrik Sioris , Meri Mäkelä , Anton Kontunen , Markus Karjalainen , Antti Vehkaoja , Niku Oksala , Antti Roine","doi":"10.1016/j.microc.2024.111733","DOIUrl":"10.1016/j.microc.2024.111733","url":null,"abstract":"<div><div>Surgical smoke analysis offers a way to provide assisting information to the surgeon intraoperatively, which can be potentially used to assess cancer tumor margins in surgical oncology and alert the operator of accidental organ injuries caused by electrosurgical (ES) instruments. Surgical smoke content is affected by the energy instrument it is produced by. Classification of surgical smoke by differential ion mobility spectrometry (DMS) was evaluated with 6 porcine tissue types and 5 energy instruments. Instruments consisted of mono- and bipolar instruments, ultrasonic shears and a –blade. Machine learning was used to classify tissues by training binary classifier linear discriminant analysis (LDA) to distinguish a marked tissue class from the rest. The greatest binary classification accuracies were obtained with the monopolar instruments and the lowest with the bipolar instrument, 93.5 % and 77.5 % respectively. The analysis of surgical smoke with DMS is possible with a variety of energy instruments, however with varying performance. This implies that DMS based tissue identification is generalizable across different surgical instruments and surgical specialties.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"207 ","pages":"Article 111733"},"PeriodicalIF":4.9,"publicationDate":"2024-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0026265X24018459/pdfft?md5=7b047d8b0257ec6cbded8f7d55647ee7&pid=1-s2.0-S0026265X24018459-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142310608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0