Microchemical Journal最新文献_第7页

Adhesive-emitted odorants detection using an electronic nose: Unraveling algorithm applicability with controlled dataset characteristics

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2025-03-02 DOI: 10.1016/j.microc.2025.113220

Chen Qu , Zhuoran Zhang , Ning Liu , Zixuan Zhao , Zhongbao Guo , Jinhua Liu , Jiemin Liu , Chuandong Wu

Electronic nose technology is becoming increasingly important in pollution monitoring; however, the inappropriate selection of pattern recognition algorithms may lead to performance decreases. In this study, an electronic nose was developed for the qualitative classification of adhesives and quantitative detection of adhesive-emitted odorant concentrations. Meanwhile, the applicability of commonly used pattern recognition algorithms (support vector regression, partial least squares regression, artificial neural network (ANN), random forest regression (RFR), ridge regression, and Lasso regression) to datasets with controlled volumes and interference intensities was investigated by comparing their quantitative performance. In qualitative analysis, the support vector machine with polynomial nonlinear kernel and random forest achieved 100% accurate classification of 11 adhesive samples. In quantitative analysis, RFR demonstrated good generalization ability and interference insensitivity, with a mean absolute percentage error (MAPE) below 3% in the large-volume strongly interfering dataset and less than 25% in the small-volume strongly interfering dataset. While ANN showed certain data volume dependence and interference sensitivity. For dataset with small volume and weak interference, algorithms with simple structures could achieve accurate quantification (MAPE of 10%). Moreover, the algorithm applicability was validated on homologous datasets and the effectiveness of cluster analysis to remove outliers was discussed.

{"title":"Adhesive-emitted odorants detection using an electronic nose: Unraveling algorithm applicability with controlled dataset characteristics","authors":"Chen Qu , Zhuoran Zhang , Ning Liu , Zixuan Zhao , Zhongbao Guo , Jinhua Liu , Jiemin Liu , Chuandong Wu","doi":"10.1016/j.microc.2025.113220","DOIUrl":"10.1016/j.microc.2025.113220","url":null,"abstract":"<div><div>Electronic nose technology is becoming increasingly important in pollution monitoring; however, the inappropriate selection of pattern recognition algorithms may lead to performance decreases. In this study, an electronic nose was developed for the qualitative classification of adhesives and quantitative detection of adhesive-emitted odorant concentrations. Meanwhile, the applicability of commonly used pattern recognition algorithms (support vector regression, partial least squares regression, artificial neural network (ANN), random forest regression (RFR), ridge regression, and Lasso regression) to datasets with controlled volumes and interference intensities was investigated by comparing their quantitative performance. In qualitative analysis, the support vector machine with polynomial nonlinear kernel and random forest achieved 100% accurate classification of 11 adhesive samples. In quantitative analysis, RFR demonstrated good generalization ability and interference insensitivity, with a mean absolute percentage error (MAPE) below 3% in the large-volume strongly interfering dataset and less than 25% in the small-volume strongly interfering dataset. While ANN showed certain data volume dependence and interference sensitivity. For dataset with small volume and weak interference, algorithms with simple structures could achieve accurate quantification (MAPE of 10%). Moreover, the algorithm applicability was validated on homologous datasets and the effectiveness of cluster analysis to remove outliers was discussed.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"212 ","pages":"Article 113220"},"PeriodicalIF":4.9,"publicationDate":"2025-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143547961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of an origami paper-based electrochemical sensor using N-doped graphene for simultaneous detection of Cd(II), Pb(II), and Hg(II) in water

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2025-03-02 DOI: 10.1016/j.microc.2025.113223

Jiaqi Wei , Linzhe Wang , Jingfang Hu , Wensong Wei , Yangchun Yang , Yu Song , Yansheng Li , Guowei Gao

Heavy metals expose great hazards to the ecological environment and public health due to their high toxicity and non-biodegradability. In this work, a low-cost, portable origami electrochemical microfluidic paper-based analytical device (EµPAD) was developed using nitrogen-doped graphene (NG) for simultaneous detection of Cd(II), Pb(II), and Hg(II) in water. The origami structure functions as a valve between sample introduction and detection, reducing sample volume and enhancing operation convenience. Electrochemical linear scanning voltammetry was employed for the first time in the in-situ synthesis of NG on paper-based electrode for sensing heavy metal ions. With its large specific surface area and numerous active sites, NG-modified electrodes exhibited excellent analytical performance for simultaneous determination of Cd(II), Pb(II), and Hg(II). The EµPAD sensor exhibited a wide linear response ranging from 5 to 100 µg/L, and low detection limits (Cd(II): 0.5698 µg/L, Pb(II): 0.4024 µg/L, Hg(II): 0.2565 µg/L,), which were well below the drinking water standards. Additionally, the EµPAD has been successfully applied to real water samples, achieving recoveries of 96.4 % ∼106.2 % with RSDs below 7 %.

{"title":"Development of an origami paper-based electrochemical sensor using N-doped graphene for simultaneous detection of Cd(II), Pb(II), and Hg(II) in water","authors":"Jiaqi Wei , Linzhe Wang , Jingfang Hu , Wensong Wei , Yangchun Yang , Yu Song , Yansheng Li , Guowei Gao","doi":"10.1016/j.microc.2025.113223","DOIUrl":"10.1016/j.microc.2025.113223","url":null,"abstract":"<div><div>Heavy metals expose great hazards to the ecological environment and public health due to their high toxicity and non-biodegradability. In this work, a low-cost, portable origami electrochemical microfluidic paper-based analytical device (EµPAD) was developed using nitrogen-doped graphene (NG) for simultaneous detection of Cd(II), Pb(II), and Hg(II) in water. The origami structure functions as a valve between sample introduction and detection, reducing sample volume and enhancing operation convenience. Electrochemical linear scanning voltammetry was employed for the first time in the in-situ synthesis of NG on paper-based electrode for sensing heavy metal ions. With its large specific surface area and numerous active sites, NG-modified electrodes exhibited excellent analytical performance for simultaneous determination of Cd(II), Pb(II), and Hg(II). The EµPAD sensor exhibited a wide linear response ranging from 5 to 100 µg/L, and low detection limits (Cd(II): 0.5698 µg/L, Pb(II): 0.4024 µg/L, Hg(II): 0.2565 µg/L,), which were well below the drinking water standards. Additionally, the EµPAD has been successfully applied to real water samples, achieving recoveries of 96.4 % ∼106.2 % with RSDs below 7 %.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"212 ","pages":"Article 113223"},"PeriodicalIF":4.9,"publicationDate":"2025-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143562398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ratiometric fluorescent detection of heparan sulfate in human plasma and serum using peptide-based fluorescent probes

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2025-03-02 DOI: 10.1016/j.microc.2025.113222

Sumita Subedi, Kishor Khadka, Myeong-geun Park, Inae Jeon, Moodong Cho, Keun-Hyeung Lee

The level of heparan sulfate (HS) in human blood acts as a biomarker for several diseases, making the development of sensitive and selective detection methods increasingly important. Until now, there has been no ratiometric fluorescent detection method for sensing HS in blood samples. In this study, we report a novel ratiometric fluorescent detection method for HS in blood samples using two peptide-based fluorescent probes: probe 1, containing six Arg residues, and probe 2, containing two Arg residues and four Lys residues. Both probes, excited with visible light at 430 nm, exhibited ratiometric fluorescence responses to HS in human plasma solutions. However, probe 1 exhibited a highly sensitive ratiometric response to HS at nanomolar concentrations (0–300 nM) in 50 % human plasma samples, whereas probe 2 exhibited ratiometric responses at nanomolar concentrations (0–800 nM) in plasma samples containing up to 30 % human plasma. Probe 1 also showed a highly selective ratiometric response to HS over heparin and other biological competitors in human plasma samples. The detection limit of probe 1 was determined to be 14.23 nM in 50 % human plasma samples. Structural analysis of the developed fluorescent probes revealed that the presence of six guanidine groups, rather than amino groups, significantly enhances HS detection in blood samples by minimizing non-specific binding to biomolecules. This work represents the first approach to quantifying HS levels in human serum and plasma samples using ratiometric fluorescence techniques, providing a promising tool for disease biomarker identification and clinical diagnostics.

{"title":"Ratiometric fluorescent detection of heparan sulfate in human plasma and serum using peptide-based fluorescent probes","authors":"Sumita Subedi, Kishor Khadka, Myeong-geun Park, Inae Jeon, Moodong Cho, Keun-Hyeung Lee","doi":"10.1016/j.microc.2025.113222","DOIUrl":"10.1016/j.microc.2025.113222","url":null,"abstract":"<div><div>The level of heparan sulfate (HS) in human blood acts as a biomarker for several diseases, making the development of sensitive and selective detection methods increasingly important. Until now, there has been no ratiometric fluorescent detection method for sensing HS in blood samples. In this study, we report a novel ratiometric fluorescent detection method for HS in blood samples using two peptide-based fluorescent probes: probe <strong>1</strong>, containing six Arg residues, and probe <strong>2</strong>, containing two Arg residues and four Lys residues. Both probes, excited with visible light at 430 nm, exhibited ratiometric fluorescence responses to HS in human plasma solutions. However, probe <strong>1</strong> exhibited a highly sensitive ratiometric response to HS at nanomolar concentrations (0–300 nM) in 50 % human plasma samples, whereas probe <strong>2</strong> exhibited ratiometric responses at nanomolar concentrations (0–800 nM) in plasma samples containing up to 30 % human plasma. Probe <strong>1</strong> also showed a highly selective ratiometric response to HS over heparin and other biological competitors in human plasma samples. The detection limit of probe <strong>1</strong> was determined to be 14.23 nM in 50 % human plasma samples. Structural analysis of the developed fluorescent probes revealed that the presence of six guanidine groups, rather than amino groups, significantly enhances HS detection in blood samples by minimizing non-specific binding to biomolecules. This work represents the first approach to quantifying HS levels in human serum and plasma samples using ratiometric fluorescence techniques, providing a promising tool for disease biomarker identification and clinical diagnostics.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"212 ","pages":"Article 113222"},"PeriodicalIF":4.9,"publicationDate":"2025-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143561915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pyridine-substituted cyanostilbene macrocycle: A “turn-on” fluorescence sensor for pesticide bromoxynil octanoate

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2025-03-02 DOI: 10.1016/j.microc.2025.113221

Pan He , Sining Zheng , Yun Li , Hongyu Guo , Fafu Yang

Pesticide bromoxynil octanoate is widely used in agriculture but possesses the potential toxicity to both animals and humans. The development of facile and on-site detection method for bromoxynil octanoate is extremely expected. In this work, the first example of fluorescent sensor for on-site examining bromoxynil octanoate in aqueous media was reported based on a pyridine-substituted cyanostilbene macrocycle (PSCM). This fluorescent sensor exhibited strong “turn-on” orange fluorescence on sensing bromoxynil octanoate with high selectivity and sensitivity among a wide range of ions and pesticides. The limitation of detection is 2.1 × 10⁻⁸ M. The study on binding mechanism suggested that bromoxynil octanoate is located in the cavity of PSCM based on π-π and hydrophobic interactions. The application experiments indicated that this fluorescent sensor has the excellent sensing behavior on test papers, four real agriculture samples and simulated water samples, implying the good application potential on facile, simple and on-site detecting bromoxynil octanoate in real environments.

{"title":"Pyridine-substituted cyanostilbene macrocycle: A “turn-on” fluorescence sensor for pesticide bromoxynil octanoate","authors":"Pan He , Sining Zheng , Yun Li , Hongyu Guo , Fafu Yang","doi":"10.1016/j.microc.2025.113221","DOIUrl":"10.1016/j.microc.2025.113221","url":null,"abstract":"<div><div>Pesticide bromoxynil octanoate is widely used in agriculture but possesses the potential toxicity to both animals and humans. The development of facile and on-site detection method for bromoxynil octanoate is extremely expected. In this work, the first example of fluorescent sensor for on-site examining bromoxynil octanoate in aqueous media was reported based on a pyridine-substituted cyanostilbene macrocycle (<strong>PSCM</strong>). This fluorescent sensor exhibited strong “turn-on” orange fluorescence on sensing bromoxynil octanoate with high selectivity and sensitivity among a wide range of ions and pesticides. The limitation of detection is 2.1 × 10<sup>−8</sup> M. The study on binding mechanism suggested that bromoxynil octanoate is located in the cavity of <strong>PSCM</strong> based on π-π and hydrophobic interactions. The application experiments indicated that this fluorescent sensor has the excellent sensing behavior on test papers, four real agriculture samples and simulated water samples, implying the good application potential on facile, simple and on-site detecting bromoxynil octanoate in real environments.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"212 ","pages":"Article 113221"},"PeriodicalIF":4.9,"publicationDate":"2025-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143534158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synthesis of Multi-Metal Oxoanion-Modified silver on renewable Potato-Starch based carbon microspheres via a ‘Ternary synergistic Strategy’ for sensitive adrenaline detection

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2025-03-01 DOI: 10.1016/j.microc.2025.113196

Zhuoxian Tang , Lin Hao , Tingyu Zhang , Hongyuan Yan , Yufan Zhang

This study introduces an innovative strategy for synthesizing low-cost, renewable carbon microspheres derived from potato starch. Uniformly dispersed potato-starch-based carbon microspheres (PCMs) were fabricated via a catalyst-free hydrothermal method, followed by calcination. Polyoxometalates (POMs) acted as both reducing and bridging agents, enabling the uniform deposition of silver nanoparticles (Ag NPs) on the PCM surface. The Ag NPs exhibited remarkable catalytic activity and a high specific surface area, effectively lowering the oxidation potential of adrenaline molecules and accelerating electron transfer, thereby enhancing detection sensitivity. As a biocarbon material, PCMs possess a high specific surface area and a porous structure, facilitating the adsorption and distribution of adrenaline molecules and thereby improving sensor performance. The synthesized ternary nanocomposite, Ag@POM/PCMs, exhibited synergistic catalytic behavior arising from the combined effects of catalytically active Ag NPs, electron-transfer-promoting POMs, and porous PCMs with a large surface area. This unique synergy significantly enhanced electrocatalytic performance, as demonstrated by high sensitivity, a broad linear detection range (0.46–1626.64 μM), and a low detection limit (0.238 μM, S/N = 3). Moreover, the material exhibited excellent stability, reproducibility, and anti-interference properties, achieving recovery rates of 99.2 % to 101.9 % in human serum samples.

{"title":"Synthesis of Multi-Metal Oxoanion-Modified silver on renewable Potato-Starch based carbon microspheres via a ‘Ternary synergistic Strategy’ for sensitive adrenaline detection","authors":"Zhuoxian Tang , Lin Hao , Tingyu Zhang , Hongyuan Yan , Yufan Zhang","doi":"10.1016/j.microc.2025.113196","DOIUrl":"10.1016/j.microc.2025.113196","url":null,"abstract":"<div><div>This study introduces an innovative strategy for synthesizing low-cost, renewable carbon microspheres derived from potato starch. Uniformly dispersed potato-starch-based carbon microspheres (PCMs) were fabricated via a catalyst-free hydrothermal method, followed by calcination. Polyoxometalates (POMs) acted as both reducing and bridging agents, enabling the uniform deposition of silver nanoparticles (Ag NPs) on the PCM surface. The Ag NPs exhibited remarkable catalytic activity and a high specific surface area, effectively lowering the oxidation potential of adrenaline molecules and accelerating electron transfer, thereby enhancing detection sensitivity. As a biocarbon material, PCMs possess a high specific surface area and a porous structure, facilitating the adsorption and distribution of adrenaline molecules and thereby improving sensor performance. The synthesized ternary nanocomposite, Ag@POM/PCMs, exhibited synergistic catalytic behavior arising from the combined effects of catalytically active Ag NPs, electron-transfer-promoting POMs, and porous PCMs with a large surface area. This unique synergy significantly enhanced electrocatalytic performance, as demonstrated by high sensitivity, a broad linear detection range (0.46–1626.64 μM), and a low detection limit (0.238 μM, S/N = 3). Moreover, the material exhibited excellent stability, reproducibility, and anti-interference properties, achieving recovery rates of 99.2 % to 101.9 % in human serum samples.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"212 ","pages":"Article 113196"},"PeriodicalIF":4.9,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143534156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Robust SPE-UHPLC-MS/MS method for determination of multiple categories of antibiotics with broad polarity range in surface water

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2025-03-01 DOI: 10.1016/j.microc.2025.113198

Xinyuan Pei , Nan Zhang , Yuting Chen , Shiyu Miao , Huiyang Fu , Qingqing Zhu , Zhengbo Dai , Yi Chi , Ligang Hu , Rong Jin , Chunyang Liao

Antibiotics, as a typical class of emerging contaminants in the environment, are frequently detected at trace concentrations in various water samples. The chemical properties of antibiotics exhibit significant diversity, encompassing variations in molecular structure, amphoteric behaviour, and polarity. These differences present substantial challenges in developing universal detection methods for comprehensive monitoring of antibiotic contaminants. Therefore, it is imperative to develop more targeted, efficient, and sensitive analytical techniques. In this study, we develop a solid-phase extraction coupled with ultra-performance liquid chromatography-tandem mass spectrometry (SPE-UHPLC-MS/MS) method. This method enables the simultaneous determination of 69 antibiotics divided into seven categories (tetracyclines, quinolones, sulfonamides, β-lactams, macrolides, lincosamides, and chloramphenicols) in surface water. The method achieved limits of detection (LOQs) ranging from 0.002 to 1.71 ng/L. The standard curves of the target compounds exhibited excellent linearity, with correlation coefficients (r²) > 0.995, and most spiked recoveries fell within the range of 40.4–149 %, with relative standard deviations (RSDs) below 15 %. Using this method, 19 surface water samples from Qiantang River Basin, Zhejiang Province, China were analyzed, with 29 out of 69 analytes being detected (the total concentrations ranging from 5.69 to 131 ng/L). This method provides a reliable and robust analytical approach for the comprehensive monitoring of antibiotic contaminants in environmental water, offering critical insights for pollution control and water quality management efforts.

{"title":"Robust SPE-UHPLC-MS/MS method for determination of multiple categories of antibiotics with broad polarity range in surface water","authors":"Xinyuan Pei , Nan Zhang , Yuting Chen , Shiyu Miao , Huiyang Fu , Qingqing Zhu , Zhengbo Dai , Yi Chi , Ligang Hu , Rong Jin , Chunyang Liao","doi":"10.1016/j.microc.2025.113198","DOIUrl":"10.1016/j.microc.2025.113198","url":null,"abstract":"<div><div>Antibiotics, as a typical class of emerging contaminants in the environment, are frequently detected at trace concentrations in various water samples. The chemical properties of antibiotics exhibit significant diversity, encompassing variations in molecular structure, amphoteric behaviour, and polarity. These differences present substantial challenges in developing universal detection methods for comprehensive monitoring of antibiotic contaminants. Therefore, it is imperative to develop more targeted, efficient, and sensitive analytical techniques. In this study, we develop a solid-phase extraction coupled with ultra-performance liquid chromatography-tandem mass spectrometry (SPE-UHPLC-MS/MS) method. This method enables the simultaneous determination of 69 antibiotics divided into seven categories (tetracyclines, quinolones, sulfonamides, β-lactams, macrolides, lincosamides, and chloramphenicols) in surface water. The method achieved limits of detection (LOQs) ranging from 0.002 to 1.71 ng/L. The standard curves of the target compounds exhibited excellent linearity, with correlation coefficients (r<sup>2</sup>) > 0.995, and most spiked recoveries fell within the range of 40.4–149 %, with relative standard deviations (RSDs) below 15 %. Using this method, 19 surface water samples from Qiantang River Basin, Zhejiang Province, China were analyzed, with 29 out of 69 analytes being detected (the total concentrations ranging from 5.69 to 131 ng/L). This method provides a reliable and robust analytical approach for the comprehensive monitoring of antibiotic contaminants in environmental water, offering critical insights for pollution control and water quality management efforts.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"212 ","pages":"Article 113198"},"PeriodicalIF":4.9,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143547890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PVDF membrane immobilized acetylcholinesterase-based fluorescence biosensor for chlorpyrifos detection

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2025-03-01 DOI: 10.1016/j.microc.2025.113197

Jia Liu , Lin Li , Xin-Yue Chen , Juan Chen

Trace residues of chlorpyrifos (organophosphorus pesticides) pose significant environmental hazards due to their acute insecticidal toxicity. Chlorpyrifos inhibits acetylcholinesterase (AChE), which heightens the risks of neurological disorders. The implications of these interactions underscore the urgent need for detection strategies to mitigate the risks associated with chlorpyrifos exposure. Considering the inherently fragile nature of AChE, a novel composite membrane (PVDF/PDA/PEI) was introduced to immobilize AChE for its excellent simplicity, portability and ease of separation. And a ratiometric fluorescence biosensor based on photoluminescent carbon dots emitting at 468 nm and cobalt oxyhydroxide nanosheets emitting at 568 nm were constructed. The resulting fluorescence ratio at F₄₆₈/F₅₆₈ was served as a ratiometric fluorescence signal for AChE assay and chlorpyrifos detection, with a detection limit of 0.103 ng/mL. This study facilitates the precise monitoring of chlorpyrifos levels in real samples, providing a reliable method to detect even trace amounts of this widely used chlorpyrifos. By offering a balance between the activity and stability of fragile AChE, facilitating the utilization of PVDF/PDA/PEI immobilized AChE in the fabrication of a ratiometric fluorescence sensor for the purpose of chlorpyrifos detection. This advancement not only improves detection sensitivity but also contributes significantly to environmental safety and public health monitoring.

{"title":"PVDF membrane immobilized acetylcholinesterase-based fluorescence biosensor for chlorpyrifos detection","authors":"Jia Liu , Lin Li , Xin-Yue Chen , Juan Chen","doi":"10.1016/j.microc.2025.113197","DOIUrl":"10.1016/j.microc.2025.113197","url":null,"abstract":"<div><div>Trace residues of chlorpyrifos (organophosphorus pesticides) pose significant environmental hazards due to their acute insecticidal toxicity. Chlorpyrifos inhibits acetylcholinesterase (AChE), which heightens the risks of neurological disorders. The implications of these interactions underscore the urgent need for detection strategies to mitigate the risks associated with chlorpyrifos exposure. Considering the inherently fragile nature of AChE, a novel composite membrane (PVDF/PDA/PEI) was introduced to immobilize AChE for its excellent simplicity, portability and ease of separation. And a ratiometric fluorescence biosensor based on photoluminescent carbon dots emitting at 468 nm and cobalt oxyhydroxide nanosheets emitting at 568 nm were constructed. The resulting fluorescence ratio at F<sub>468</sub>/F<sub>568</sub> was served as a ratiometric fluorescence signal for AChE assay and chlorpyrifos detection, with a detection limit of 0.103 ng/mL. This study facilitates the precise monitoring of chlorpyrifos levels in real samples, providing a reliable method to detect even trace amounts of this widely used chlorpyrifos. By offering a balance between the activity and stability of fragile AChE, facilitating the utilization of PVDF/PDA/PEI immobilized AChE in the fabrication of a ratiometric fluorescence sensor for the purpose of chlorpyrifos detection. This advancement not only improves detection sensitivity but also contributes significantly to environmental safety and public health monitoring.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"212 ","pages":"Article 113197"},"PeriodicalIF":4.9,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143547820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficient detection and validation of 4-Methoxyphenol chemical based on binary oxides intercalated ZnO-CdO nanocomposite by electrochemical approach 基于二元氧化物插层 ZnO-CdO 纳米复合材料的电化学方法对 4-甲氧基苯酚化学物质的高效检测和验证

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2025-03-01 DOI: 10.1016/j.microc.2025.113171

Umer Shahzad , Hadi M. Marwani , Muhammad Fazle Rabbee , Maha G. Batterjee , Jehan Y. Al-Humaidi , Zahoor Ahmad , Mohammad Mizanur Rahman Khan , Mohammed M. Rahman

In this approach, nanoscale binary doped materials was prepared by solid state method as Zinc oxide intercalated Cadmium oxide nanocomposites (ZnO-CdO NCs) and then practically implemented for the validation and electrochemical analyses using linear sweep voltammetry (LSV) and cyclic voltametric (CV) methods. Out of all the substances that were causing interferences, the constructed sensor probe showed selectivity towards 4-methoxyphenol (4-MP) due to the molecular interaction onto the intercalated surfaces of ZnO-CdO NCs. The chemical sensor exhibited enhanced electrochemical characteristics, such as higher sensitivity, a wide linear dynamic range (LDR), lower limit of detection (LOD), and good stability in detecting the specific compound 4-MP. For their optical, elemental, functional, morphological, and structural properties, the calcined ZnO-CdO NCs were analyzed using FTIR, UV, FESEM, EDS, XPS, TEM, and XRD techniques, respectively. In sensor fabrication, a flat GCE was constructed by applying a thin layer of NCs using a 5 % Nafion binder as a chemical coating agent. The calibration graph exhibited a linear relationship with a high coefficient of determination (r² = 0.9680) throughout a broad range of 4-MP concentrations (0.22–7.5 µM). The sensitivity and limit of detection (LOD) were determined to be 1.5189 μAmM⁻¹ cm⁻² and 0.01091 µM respectively. The synthesis of ZnO-CdO NCs by solid-state method is a promising approach for developing a phenolic sensor in a broad scale by electrochemical approach. The 4-MP sensor has demonstrated a promising performance using ZnO-CdO NCs by LSV and CV methods. This significantly impacts the identification of hazardous phenolic chemicals by electrochemical methods in the environmental and healthcare sectors, hence ensuring safety.

{"title":"Efficient detection and validation of 4-Methoxyphenol chemical based on binary oxides intercalated ZnO-CdO nanocomposite by electrochemical approach","authors":"Umer Shahzad , Hadi M. Marwani , Muhammad Fazle Rabbee , Maha G. Batterjee , Jehan Y. Al-Humaidi , Zahoor Ahmad , Mohammad Mizanur Rahman Khan , Mohammed M. Rahman","doi":"10.1016/j.microc.2025.113171","DOIUrl":"10.1016/j.microc.2025.113171","url":null,"abstract":"<div><div>In this approach, nanoscale binary doped materials was prepared by solid state method as Zinc oxide intercalated Cadmium oxide nanocomposites (ZnO-CdO NCs) and then practically implemented for the validation and electrochemical analyses using linear sweep voltammetry (LSV) and cyclic voltametric (CV) methods. Out of all the substances that were causing interferences, the constructed sensor probe showed selectivity towards 4-methoxyphenol (4-MP) due to the molecular interaction onto the intercalated surfaces of ZnO-CdO NCs. The chemical sensor exhibited enhanced electrochemical characteristics, such as higher sensitivity, a wide linear dynamic range (LDR), lower limit of detection (LOD), and good stability in detecting the specific compound 4-MP. For their optical, elemental, functional, morphological, and structural properties, the calcined ZnO-CdO NCs were analyzed using FTIR, UV, FESEM, EDS, XPS, TEM, and XRD techniques, respectively. In sensor fabrication, a flat GCE was constructed by applying a thin layer of NCs using a 5 % Nafion binder as a chemical coating agent. The calibration graph exhibited a linear relationship with a high coefficient of determination (r<sup>2</sup> = 0.9680) throughout a broad range of 4-MP concentrations (0.22–7.5 µM). The sensitivity and limit of detection (LOD) were determined to be 1.5189 μAmM<sup>−1</sup> cm<sup>−2</sup> and 0.01091 µM respectively. The synthesis of ZnO-CdO NCs by solid-state method is a promising approach for developing a phenolic sensor in a broad scale by electrochemical approach. The 4-MP sensor has demonstrated a promising performance using ZnO-CdO NCs by LSV and CV methods. This significantly impacts the identification of hazardous phenolic chemicals by electrochemical methods in the environmental and healthcare sectors, hence ensuring safety.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"212 ","pages":"Article 113171"},"PeriodicalIF":4.9,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143561912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sr-doped MnO2 with enhanced dual-enzyme mimetic activity for colorimetric detection of glutathione and 2,4-dichlorophenol 掺锶二氧化锰具有增强的双酶模拟活性，可用于谷胱甘肽和 2,4-二氯苯酚的比色检测

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2025-03-01 DOI: 10.1016/j.microc.2025.113166

Wei Shi , Xueyi Wang , Zhouping Yang , Jun Yang , Xiaoying Fu , Xianxiang Wang

The development of simple and rapid colorimetric methods for monitoring food nutrition and safety is essential for sustaining life and promoting health. Nanozymes, which exhibit high stability and multi-enzyme activity, hold significant promise for widespread applications in colorimetric analysis. In this work, we successfully synthesized strontium-doped MnO₂ nanoflowers (Sr-MnO₂ NFs) that exhibit both oxidase-like and laccase-like activities. The oxidase activity and laccase activity of the Sr-MnO₂ NFs were 2.12 and 3 times greater than those of MnO₂ nanorods (MnO₂ NRs), respectively. Oxygen vacancies (Ov) and O₂⁻ play a key role in oxidase-like and laccase-like enzymes. Leveraging the excellent catalytic properties of these activities, we developed a simple, fast and highly sensitive colorimetric detection method for glutathione (GSH) and 2,4-dichlorophenol (2,4-DP), achieving low detection limits of 0.46 μM and 0.992 μM, respectively. Furthermore, the proposed method has been successfully applied to the determination of GSH and 2,4-DP in food samples.

{"title":"Sr-doped MnO2 with enhanced dual-enzyme mimetic activity for colorimetric detection of glutathione and 2,4-dichlorophenol","authors":"Wei Shi , Xueyi Wang , Zhouping Yang , Jun Yang , Xiaoying Fu , Xianxiang Wang","doi":"10.1016/j.microc.2025.113166","DOIUrl":"10.1016/j.microc.2025.113166","url":null,"abstract":"<div><div>The development of simple and rapid colorimetric methods for monitoring food nutrition and safety is essential for sustaining life and promoting health. Nanozymes, which exhibit high stability and multi-enzyme activity, hold significant promise for widespread applications in colorimetric analysis. In this work, we successfully synthesized strontium-doped MnO<sub>2</sub> nanoflowers (Sr-MnO<sub>2</sub> NFs) that exhibit both oxidase-like and laccase-like activities. The oxidase activity and laccase activity of the Sr-MnO<sub>2</sub> NFs were 2.12 and 3 times greater than those of MnO<sub>2</sub> nanorods (MnO<sub>2</sub> NRs), respectively. Oxygen vacancies (Ov) and O<sub>2</sub><sup><img>−</sup> play a key role in oxidase-like and laccase-like enzymes. Leveraging the excellent catalytic properties of these activities, we developed a simple, fast and highly sensitive colorimetric detection method for glutathione (GSH) and 2,4-dichlorophenol (2,4-DP), achieving low detection limits of 0.46 μM and 0.992 μM, respectively. Furthermore, the proposed method has been successfully applied to the determination of GSH and 2,4-DP in food samples.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"212 ","pages":"Article 113166"},"PeriodicalIF":4.9,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143561914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

NiWO4 with oxygen vacancy induced Au nanoparticle signal amplification for electrochemical detection of swine fever virus

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2025-03-01 DOI: 10.1016/j.microc.2025.113207

Shiyu Jiang , Xiaohong Fu , Min Yang , Jiageng Li , Guowei Deng , Shuyong Shang , Ruibo Qin , Qiang Zhang

Classical swine fever virus (CSFV) represents a significant threat to the pig industry, capable of causing devastating infectious diseases.. It is an urgent need to identify and quantify viruses with a rapid, sensitive and accurate technology. Integration of nanotechnology will allow the development of microdevices combined with electrochemical technology for efficient virus detection. Here, the electrochemical characteristics of MWCNTs-decorated Au-NiWO₄ are studied, and an ideal nanomaterial is constructed for immunosensor of CSFV antigen identifying based on steric effects about oxygen vacancy coupling CSFV enhanced reactant adsorption. The developed immunosensor detects CSFV over a range of 2 × 10^-13μg·mL⁻¹-8 × 10^-9μg·mL⁻¹, and with a detection limit of 1.58 × 10^-13 μg·mL⁻¹. In addition, AuNPs/NiWO₄/Ć shows an excellent linear relationship at the scanning speed of 10 ∼ 100 mv/s, and the relative standard deviation (RSD) was found to be only 0.60 %. The synergistic integration of MWCNTs/GO, AuNPs and electroactive Au-NiWO₄ provides superior signal response when used for the quantification of CSFV from CSFV antigen samples, suggesting the application prospects of this nanomaterial in detection.

{"title":"NiWO4 with oxygen vacancy induced Au nanoparticle signal amplification for electrochemical detection of swine fever virus","authors":"Shiyu Jiang , Xiaohong Fu , Min Yang , Jiageng Li , Guowei Deng , Shuyong Shang , Ruibo Qin , Qiang Zhang","doi":"10.1016/j.microc.2025.113207","DOIUrl":"10.1016/j.microc.2025.113207","url":null,"abstract":"<div><div>Classical swine fever virus (CSFV) represents a significant threat to the pig industry, capable of causing devastating infectious diseases.. It is an urgent need to identify and quantify viruses with a rapid, sensitive and accurate technology. Integration of nanotechnology will allow the development of microdevices combined with electrochemical technology for efficient virus detection. Here, the electrochemical characteristics of MWCNTs-decorated Au-NiWO<sub>4</sub> are studied, and an ideal nanomaterial is constructed for immunosensor of CSFV antigen identifying based on steric effects about oxygen vacancy coupling CSFV enhanced reactant adsorption. The developed immunosensor detects CSFV over a range of 2 × 10<sup>-13</sup>μg·mL<sup>−1</sup>-8 × 10<sup>-9</sup>μg·mL<sup>−1</sup>, and with a detection limit of 1.58 × 10<sup>-13</sup> μg·mL<sup>−1</sup>. In addition, AuNPs/NiWO<sub>4</sub>/Ć shows an excellent linear relationship at the scanning speed of 10 ∼ 100 mv/s, and the relative standard deviation (RSD) was found to be only 0.60 %. The synergistic integration of MWCNTs/GO, AuNPs and electroactive Au-NiWO<sub>4</sub> provides superior signal response when used for the quantification of CSFV from CSFV antigen samples, suggesting the application prospects of this nanomaterial in detection.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"212 ","pages":"Article 113207"},"PeriodicalIF":4.9,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143548560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0