Microchemical Journal最新文献_第9页

Nanomaterials-enabled dual/triple-mode optical sensing: recent advances (2020–2025) in multimodal analysis 纳米材料支持的双/三模光学传感：多模态分析的最新进展（2020-2025）

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2026-01-01 DOI: 10.1016/j.microc.2025.116759

Baozhen Hao , Xinyi Yao , Kai Li, Xiaoyang Lu, Xinyu Feng, Weicheng Sun, Sha Liu

Conventional single-mode optical methods suffer from low sensitivity, poor anti-interference, and high false-positive rates in complex matrices, failing trace, rapid and on-site detection needs. Multi-mode sensing emerges as a key solution by integrating signals for self-calibration and complementary analysis. Research over the past five years indicates that nanomaterials-based dual/triple-mode optical sensors generally achieve 1–3 orders of magnitude higher detection sensitivity and 10–20 % shorter detection times compared to conventional single-mode optical sensors. This significantly enhances the efficiency of trace analysis and on-site detection. Leveraging quantum size effects, plasmon resonance, and interfacial synergy, nanomaterials serve as core building blocks for efficient multimodal signal coupling and modulation. Focusing on 2020–2025 advances, the paper reviews quantum dots, metal oxide nanomaterials, metal nanomaterials, organic framework nanomaterials and composite nanomaterials in dual/triple-mode optical sensing. Based on the dual/triple-mode optical signal modulation of nanomaterials, this review summarizes the working principles and performance characteristics of various optical sensing strategies, including colorimetric-fluorescence, SERS-fluorescence, SERS-colorimetric and electrochemiluminescence-colorimetric/fluorescence. These sensing strategies combine high efficiency with high sensitivity, enabling fast and accurate detection to achieve real-time monitoring of analytes within complex biological systems. By comparing the advantages and disadvantages of different nanomaterials and their optical sensing strategies, a comprehensive overview of recent advances and current challenges is presented, and valuable insights into future research directions are provided. This review is expected to propel the development of efficient multimodal optical sensing technologies, advancing precision in biosensing, improving efficiency in environmental monitoring, and enhancing portability for point-of-care diagnostics.

传统的单模光学方法存在灵敏度低、抗干扰性差、在复杂矩阵中假阳性率高、无法跟踪、快速和现场检测等问题。多模传感通过集成信号进行自校准和互补分析而成为关键的解决方案。过去五年的研究表明，基于纳米材料的双/三模光学传感器与传统的单模光学传感器相比，检测灵敏度一般提高1-3个数量级，检测时间缩短10 - 20%。这大大提高了痕量分析和现场检测的效率。利用量子尺寸效应、等离子体共振和界面协同作用，纳米材料作为高效多模态信号耦合和调制的核心构建模块。针对2020-2025年的研究进展，综述了量子点、金属氧化物纳米材料、金属纳米材料、有机框架纳米材料和复合纳米材料在双/三模光学传感中的应用。基于纳米材料的双/三模光信号调制，综述了各种光传感策略的工作原理和性能特点，包括比色-荧光、sers -荧光、sers -比色和电化学发光-比色/荧光。这些传感策略结合了高效率和高灵敏度，能够快速准确地检测，实现对复杂生物系统中分析物的实时监测。通过比较不同纳米材料及其光学传感策略的优缺点，全面概述了其最新进展和面临的挑战，并对未来的研究方向提出了有价值的见解。本文综述有望推动高效多模态光学传感技术的发展，提高生物传感的精度，提高环境监测的效率，增强即时诊断的便携性。

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引用次数: 0

Development of Silica nanoparticles incorporated in 2D Ti3C2Tx MXene based Electrochemical Sensor for Gatifloxacin Detection 二氧化硅纳米颗粒复合二维Ti3C2Tx MXene电化学传感器的研制

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2026-01-01 DOI: 10.1016/j.microc.2025.116782

Nevil Pious , Sudip Das , Arghya Chakravorty , Aarcha Appu Mini , George Jacob , Ramesh Rajendran , T. Pazhanivel , Vimala Raghavan

The increasing environmental presence of gatifloxacin, a potent fluoroquinolone antibiotic, in environmental and pharmaceutical samples, is of growing concern due to its role in promoting antimicrobial resistance (AMR). Addressing this challenge, a robust electrochemical sensor was developed using a glassy carbon electrode (GCE) modified with a Ti₃C₂T_x MXene/SiO₂ composite. Ti₃C₂T_x MXene was synthesized via hydrofluoric acid etching of Ti₃AlC₂ MAX phase, followed by DMSO-assisted delamination, while SiO₂ nanoparticles were prepared by sol-gel method. Structural and morphological analyses using XRD and FE-SEM confirmed successful phase transformation and composite formation, with SiO₂ nanoparticles uniformly distributed over the exfoliated MXene layers. Among various compositions tested, the optimized MXene:SiO₂ ratio of 1:2 demonstrated superior electrochemical performance. Cyclic voltammetry (CV), differential pulse voltammetry (DPV), and square wave voltammetry (SWV) revealed a wide linear detection range from 1.553 μM to 2823.83 μM and a low limit of detection (LOD) of 0.62 μM (DPV). Electrochemical impedance spectroscopy (EIS) confirmed enhanced charge transfer characteristics, and mechanistic analysis suggested a diffusion-controlled, irreversible two-electron, two-proton oxidation of gatifloxacin, maximized at pH 8. The effective surface area was calculated as 0.0144 cm², with an estimated diffusion coefficient of 3.91 × 10⁻⁵ cm²/s. Validation with real samples, including eye drops and milk, yielded recovery rates of 100.7 % and 55.6 % respectively, demonstrating robust practical applicability. Compared to conventional analytical methods, this Ti₃C₂T_x/SiO₂/GCE sensor offered a cost-effective, rapid, and highly sensitive alternative for monitoring gatifloxacin in clinical and environmental settings, contributing meaningfully to AMR mitigation and environmental health monitoring.

加替沙星是一种强效氟喹诺酮类抗生素，在环境和药物样品中越来越多地存在于环境中，由于其在促进抗菌素耐药性（AMR）方面的作用而日益引起关注。为了解决这一挑战，研究人员使用Ti3C2Tx MXene/SiO2复合材料修饰的玻碳电极（GCE）开发了一种强大的电化学传感器。通过氢氟酸蚀刻Ti3AlC2 MAX相合成Ti3C2Tx MXene，然后采用dmso辅助分层，采用溶胶-凝胶法制备SiO2纳米颗粒。XRD和FE-SEM的结构和形态分析证实了SiO₂纳米颗粒在剥离的MXene层上均匀分布，相变成功，复合材料形成。在测试的各种成分中，优化后的MXene:SiO2比为1:2的材料表现出优异的电化学性能。循环伏安法（CV）、差分脉冲伏安法（DPV）和方波伏安法（SWV）的线性检测范围为1.553 ~ 2823.83 μM，低检出限（LOD）为0.62 μM （DPV）。电化学阻抗谱（EIS）证实了电荷转移特性的增强，机理分析表明加替沙星发生了扩散控制、不可逆的双电子、双质子氧化，在pH值为8时达到最大。有效表面积为0.0144 cm2，扩散系数为3.91 × 10−5 cm2/s。实际样品（包括滴眼液和牛奶）的验证回收率分别为100.7%和55.6%，具有较强的实用性。与传统的分析方法相比，该Ti3C2Tx/SiO2/GCE传感器为临床和环境环境中监测加替沙星提供了一种经济、快速、高灵敏度的替代方法，为减轻AMR和环境健康监测做出了有意义的贡献。

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引用次数: 0

Emerging microchemical sensors for real-time point-of-care detection of chronic kidney disease biomarkers 用于慢性肾脏疾病生物标志物实时检测的新兴微化学传感器

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2026-01-01 DOI: 10.1016/j.microc.2025.116751

Sudip Chattopadhyay

Chronic kidney disease (CKD) is a global health challenge characterized by a gradual loss of kidney function, often progressing to end-stage renal disease if untreated. This review explores the primary causes and stages of CKD, shedding light on its complex etiology, including diabetes, hypertension, and genetic predispositions. Biomarkers such as creatinine, cystatin C, and albumin play a crucial role in CKD diagnosis and progression monitoring. Emerging detection techniques leveraging sensor-based technologies are transforming the diagnostic landscape, offering advantages such as real-time monitoring, portability, and cost-effectiveness compared to conventional laboratory methods. Electrochemical, optical, and nanomaterial-based sensors are highlighted for their potential in detecting CKD biomarkers with high sensitivity and specificity. Additionally, this review delves into the socioeconomic implications of CKD, emphasizing the need for accessible and affordable diagnostic solutions. A critical comparison is drawn between commercial diagnostic techniques and point-of-care (POC) technologies, underscoring the growing preference for the latter in resource-limited settings. This review concludes by emphasizing the transformative impact of these innovations on early diagnosis and effective management of CKD, paving the way for improved patient outcomes and reduced healthcare burdens.

慢性肾脏疾病（CKD）是一种全球性的健康挑战，其特征是肾功能逐渐丧失，如果不及时治疗，通常会发展为终末期肾脏疾病。这篇综述探讨了慢性肾病的主要原因和分期，揭示了其复杂的病因，包括糖尿病、高血压和遗传易感性。生物标志物如肌酐、胱抑素C和白蛋白在CKD诊断和进展监测中起着至关重要的作用。利用基于传感器的新兴检测技术正在改变诊断领域，与传统的实验室方法相比，它具有实时监测、便携性和成本效益等优势。电化学、光学和纳米材料传感器在检测CKD生物标志物方面具有很高的灵敏度和特异性。此外，本综述深入探讨了慢性肾病的社会经济影响，强调需要可获得和负担得起的诊断解决方案。在商业诊断技术和即时护理（POC）技术之间进行了重要的比较，强调了在资源有限的情况下，后者越来越受到青睐。本综述最后强调了这些创新对CKD早期诊断和有效管理的变革性影响，为改善患者预后和减轻医疗负担铺平了道路。

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引用次数: 0

Perpetual progress in multidimensional chromatographic and mass spectrometric strategies for the resolution of complex matrices: challenges, innovations, and bioanalytical applications 用于复杂基质的多维色谱和质谱分析策略的持续进展：挑战、创新和生物分析应用

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2026-01-01 DOI: 10.1016/j.microc.2025.116741

Jinal Ajabiya , Sohan G. Jawarkar , Akash Rajput , Pinaki Sengupta

Liquid chromatography (LC) and Mass spectrometry (MS) are crucial techniques used for the identification, separation, and quantification of analytes. The combination of LC and MS provides an effective analytical strategy for handling complex chemical and biological matrices. Despite advancements in these technologies that enhance their analytical capabilities, traditional one-dimensional LC and MS approaches face critical limitations when analysing highly complex samples. Key challenges include the inadequate separation of closely related compounds and the detection of trace quantities, as well as the adverse effects of matrix components on quantitation accuracy. These issues become particularly pronounced in bioanalytical, nutraceutical, and pharmaceutical samples. Furthermore, metabolomics and proteomics samples possess wide heterogeneity, leading to poor reproducibility and sensitivity, compounded by ion suppression effects. The limitations of existing techniques are exacerbated by the static nature of the stationary and mobile phases, along with conventional ionisation methods in the case of MS. Recent innovations are exploring multidimensional chromatographic techniques, particularly two-dimensional high-performance liquid chromatography (2D-HPLC) and two-dimensional mass spectrometry (2D-MS), which enhance peak capacity and resolving power, effectively addressing matrix interferences. These multidimensional approaches facilitate comprehensive profiling in metabolomics, allowing for a broader dynamic range, and in proteomics, provide rapid and precise analysis of peptides and proteins. This article reviews the significant advancements in multidimensional chromatographic methods and their application in addressing the challenges posed by complex biological matrices, detailing both one-dimensional and two-dimensional methodologies in bioanalytical contexts.

液相色谱（LC）和质谱（MS）是用于鉴定、分离和定量分析的关键技术。液相色谱和质谱的结合为处理复杂的化学和生物基质提供了一种有效的分析策略。尽管这些技术的进步增强了它们的分析能力，传统的一维LC和MS方法在分析高度复杂的样品时面临着关键的限制。主要挑战包括密切相关化合物的分离不足和痕量的检测，以及基质成分对定量准确性的不利影响。这些问题在生物分析、营养和制药样品中尤为明显。此外，代谢组学和蛋白质组学样品具有广泛的异质性，导致再现性和敏感性差，加之离子抑制效应。固定相和流动相的静态特性以及ms中传统的电离方法加剧了现有技术的局限性。最近的创新正在探索多维色谱技术，特别是二维高效液相色谱（2D-HPLC）和二维质谱（2D-MS），它们增强了峰值容量和分辨率，有效地解决了基质干扰问题。这些多维方法促进了代谢组学的全面分析，允许更广泛的动态范围，而在蛋白质组学中，提供了快速和精确的肽和蛋白质分析。本文回顾了多维色谱方法的重大进展及其在解决复杂生物基质所带来的挑战方面的应用，详细介绍了生物分析背景下的一维和二维方法。

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引用次数: 0

A review of photothermal spectroscopy for gas sensing 光热光谱用于气体传感的研究进展

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2026-01-01 DOI: 10.1016/j.microc.2025.116716

Lei Zhu, Fu Wan, Yaotian Bai, Hongcheng Sun, Yongqi Wu, Weigen Chen

Ultra-sensitive trace gas detection is vital for applications in environmental protection, medical diagnosis, and condition monitoring of electrical equipment. Photothermal spectroscopy (PTS), characterised by high sensitivity, large dynamic range, and background-free operation, has become a very promising method for trace gas sensing. In this article, we review advancements in PTS gas sensing technology from two key perspectives: commonly used optical interferometers and signal enhancement methods. Primary optical interferometers discussed are Mach-Zehnder, Fabry-Pérot, Sagnac, and Fibre modal interferometers. Signal enhancement methods include cavity, fibre-optic, quartz tuning fork, and on-chip waveguide enhancement. These interferometer configurations and enhancement methods together advance highly precise photothermal detection. Finally, we outline future research directions to further improve the detection performance of PTS gas sensing technology, building on current foundations. This work lays the groundwork for developing robust, sensitive, and user-friendly gas analysis instruments in the future.

超灵敏微量气体检测在环境保护、医疗诊断、电气设备状态监测等领域具有重要的应用价值。光热光谱（PTS）具有灵敏度高、动态范围大、无背景等特点，是一种很有前途的痕量气体检测方法。本文从常用的光学干涉仪和信号增强两方面综述了PTS气敏技术的研究进展。主要讨论的光学干涉仪有Mach-Zehnder、fabry - p、Sagnac和光纤模态干涉仪。信号增强方法包括腔、光纤、石英音叉和片上波导增强。这些干涉仪配置和增强方法共同推进了高精度光热探测。最后，在现有基础上，提出了进一步提高PTS气敏技术检测性能的未来研究方向。这项工作为将来开发坚固、灵敏、用户友好的气体分析仪器奠定了基础。

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引用次数: 0

Molecular imprinting technology in detection and adsorption of environmental pollutants: Synthesis, mechanisms and applications 分子印迹技术在环境污染物检测和吸附中的应用：合成、机理和应用

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2026-01-01 DOI: 10.1016/j.microc.2025.116747

Dayong Tian , Menglei Zhao , Angzhan Li , Daping Zhang , Shaopeng Zhang , Liu Cheng , Chao Liu , Xiaolong Wang , Li Wang

Molecular imprinting has emerged as a powerful strategy for constructing functional polymeric materials with molecular-level selectivity. Molecularly imprinted polymers (MIPs) possess tailor-made recognition sites, high chemical and thermal stability, and cost-effective synthesis, making them particularly attractive for environmental remediation and pollutant monitoring. This review provides a comprehensive overview of recent advances in imprinting strategies, emphasizing progress in template design, monomer selection, and structural engineering for the removal and detection of metal ions, organic pollutants, and perfluorinated compounds. Quantitatively, adsorption capacities of modern MIPs have improved from tens to several hundred mg g⁻¹, while imprinting factors often exceed 5, reflecting enhanced binding efficiency and site uniformity. Emerging approaches, such as dual-template and dual-monomer imprinting, ionic-liquid-assisted systems, and carbonized polymer dot (CPDs)-based functional monomers, enable more homogeneous recognition cavities and improved selectivity under complex environmental conditions. Mechanistic discussions highlight the synergistic roles of electrostatic, hydrogen-bonding, and π–π interactions, as well as the influence of crosslinking chemistry on polymer morphology and performance. Collectively, these developments outline design principles for next-generation MIPs featuring higher sensitivity, reusability, and environmental compatibility, providing a roadmap for their integration into sustainable pollutant detection and purification technologies.

分子印迹已成为构建具有分子水平选择性的功能高分子材料的一种强有力的策略。分子印迹聚合物（MIPs）具有定制的识别位点、高化学和热稳定性以及高成本效益的合成，使其在环境修复和污染物监测方面特别具有吸引力。本文综述了印迹技术的最新进展，重点介绍了印迹技术在模板设计、单体选择和结构工程等方面的进展，以去除和检测金属离子、有机污染物和全氟化合物。从数量上看，现代mip的吸附能力从几十mg g−1提高到几百mg g−1，而印迹因子通常超过5，反映了增强的结合效率和位点均匀性。新兴的方法，如双模板和双单体印迹、离子液体辅助系统和基于碳化聚合物点（CPDs）的功能单体，可以在复杂的环境条件下实现更均匀的识别腔和更高的选择性。机理讨论强调静电、氢键和π -π相互作用的协同作用，以及交联化学对聚合物形态和性能的影响。总的来说，这些发展概述了下一代MIPs的设计原则，具有更高的灵敏度，可重用性和环境兼容性，为其集成到可持续的污染物检测和净化技术提供了路线图。

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引用次数: 0

Recent advances in organic fluorescence sensors for the detection of Hg2+ and bioimaging applications: a review 有机荧光传感器在Hg2+检测和生物成像中的应用进展

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2026-01-01 DOI: 10.1016/j.microc.2025.116779

Muhanad Alhujaily , Alaa Shafie

Heavy metal contamination, particularly mercury, is a significant environmental and health concern due to its toxicity and bioaccumulation in living organisms. Among various form of mercury, ionic forms (Hg²⁺) is one of the most hazardous, impacting ecosystems, aquatic life, and human health. Hence, the demand for accessible, sensitive, and efficient detection methods is essential. Fluorimetric probes have become powerful analytical tools for monitoring Hg²⁺ due to their exceptional sensitivity, selectivity, and ability to provide rapid, real-time responses. A wide range of organic fluorescent sensorssuch as rhodamine-, coumarin-, and pyrene-based systems have been developed to detect Hg²⁺ with high reliability and minimal interference from coexisting metal ions. Beyond simple detection, many of these probes have shown strong promise in fluorescence bioimaging. Their biocompatibility and responsive optical properties allow visualization of Hg²⁺ distribution and dynamics within living cells, tissues, and even whole organisms. This review provides a comprehensive overview of recent progress in organic fluorescent probes for Hg²⁺ sensing and bioimaging. It highlights key structural design principles, sensing mechanisms, and practical applications, with particular empHasis on how these probes contribute to advancing biomedical and environmental mercury research.

重金属污染，特别是汞，由于其毒性和在活生物体中的生物积累，是一个重大的环境和健康问题。在各种形式的汞中，离子形式（Hg2+）是最危险的汞之一，影响生态系统、水生生物和人类健康。因此，对方便、灵敏和有效的检测方法的需求是必不可少的。荧光探针由于其卓越的灵敏度、选择性和提供快速、实时响应的能力，已成为监测Hg2+的强大分析工具。广泛的有机荧光传感器，如罗丹明-，香豆素-和芘为基础的系统已经开发出检测Hg2+具有高可靠性和最小的干扰共存的金属离子。除了简单的检测之外，许多这些探针在荧光生物成像中显示出强大的前景。它们的生物相容性和响应光学特性允许可视化Hg2+在活细胞、组织甚至整个生物体中的分布和动态。本文综述了用于Hg2+传感和生物成像的有机荧光探针的最新进展。它强调了关键的结构设计原理、传感机制和实际应用，特别强调了这些探针如何促进生物医学和环境汞研究。

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引用次数: 0

Revolutionizing blood typing with microfluidics: Toward automated, low-cost, and point-of-care devices 用微流体技术革新血型：走向自动化、低成本和即时护理设备

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2026-01-01 DOI: 10.1016/j.microc.2025.116706

Amir Hossein Khodabandehloo, Mohamad Mahdi Khajeh, Maryam Saadatmand

Blood transfusion is known as the first and most prevalent protocol in cell therapy. Considering the importance of blood transfusion in clinical practice and the vital role of red blood cells (RBCs) in hemotransfusion, the incompatible transfusion of RBCs into a new body may lead to hemolysis and potentially fatal outcomes for recipients. Fortunately, many easy-to-interpret methods have been developed for deciphering the results of blood typing tests, including visual interpretations, to rule out any incompatible blood transfusions before transferring the donated blood. Currently, the field of microfluidics has opened fascinating opportunities to use as small volumes of reagents as possible for blood typing on various platforms such as paper, discs, chip. Moreover, microfluidic systems offer strong differentiation for detection, allowing precise results. Last but not least, with a focus on low reagent consumption and the ability to use minimal samples, such as fingertip samples, microfluidics presents an outstanding outlook for replacing conventional blood typing methods in laboratories and commercial devices without needing technician intervention. This method also holds great potential for coupling with artificial intelligence (AI) and machine learning (ML) algorithms for real-time interpretation. In this review, a comprehensive study was conducted on microfluidics-based approaches for blood typing across several presented platforms to date, considering the advantages, benefits, and drawbacks of microfluidics.

输血被认为是细胞治疗的第一个也是最普遍的方案。考虑到输血在临床实践中的重要性和红细胞在输血中的重要作用，红细胞不相容输注到一个新的身体可能导致溶血和潜在的致命后果。幸运的是，已经开发了许多易于解释的方法来破译血型测试的结果，包括视觉解释，以排除任何不相容的输血，然后再输送捐献的血液。目前，微流体领域为在各种平台（如纸张、光盘、芯片）上使用尽可能小体积的试剂进行血型检测提供了令人着迷的机会。此外，微流体系统为检测提供了强大的差异化，允许精确的结果。最后但并非最不重要的是，由于关注低试剂消耗和使用最小样本（如指尖样本）的能力，微流体在实验室和商业设备中取代传统的血型方法而不需要技术人员干预的前景很好。该方法还具有与人工智能（AI）和机器学习（ML）算法耦合进行实时解释的巨大潜力。在这篇综述中，考虑到微流体的优点、优点和缺点，对迄今为止几种现有平台上基于微流体的血型方法进行了全面的研究。

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引用次数: 0

Designing a high-performance Electrochemiluminescence sensor for 3-MCPD using molecularly imprinted polyaniline and computational interaction energy analysis 利用分子印迹聚苯胺和计算相互作用能分析设计高性能3-MCPD电化学发光传感器

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2026-01-01 DOI: 10.1016/j.microc.2025.116781

Eni Sutanti , Muhamad Abdulkadir Martoprawiro , Harmesa Harmesa , Afiten Rahmin Sanjaya , Isnaini Rahmawati , Yasuaki Einaga , Tribidasari Anggraningrum Ivandini

A highly sensitive electrochemiluminescence (ECL) sensor for 3-monochloropropane-1,2-diol (3-MCPD) was successfully developed using a [Ru(bpy)₃]²⁺/triethylamine system integrated with the molecularly imprinted polymer (MIP) technique. Polyaniline (PANI) was selected to modify the screen-printed carbon electrode (SPCE) to provide an active site that facilitates ion mobility and charge transfer in the biocompatibility interactions. The molecular interaction between PANI and 3-MCPD was evaluated using density functional theory (DFT), which revealed a favourable interaction energy, confirming the strong affinity and high selectivity of the proposed sensor toward 3-MCPD. In the ECL system, 3-MCPD efficiently quenched the luminescence of [Ru(bpy)₃]²⁺, indicating its strong interaction within the sensing mechanism. The sensor achieved a detection limit of 21.68 nM with a sensitivity of 0.22 a.u. nM⁻¹ mm⁻², confirming its high analytical performance for 3-MCPD detection. High ECL selectivity was achieved through integration with the MIP approach, enhancing target molecular recognition and offering a new pathway for designing robust sensors for food contaminant monitoring. Moreover, the integration of computational prediction with experimental validation provides a robust strategy for designing selective and sensitive detection platforms. The proposed sensor was successfully applied for 3-MCPD detection in a cooking oil matrix, demonstrating its applicability in spiked oil matrices under controlled laboratory conditions.

采用[Ru（bpy）₃]2+/三乙胺体系结合分子印迹聚合物（MIP）技术，成功研制了3-一氯丙烷-1,2-二醇（3-MCPD）高灵敏度电化学发光（ECL）传感器。选择聚苯胺（PANI）修饰丝网印刷碳电极（SPCE），以提供在生物相容性相互作用中促进离子迁移和电荷转移的活性位点。利用密度泛函理论（DFT）评价了PANI与3-MCPD之间的分子相互作用，发现了良好的相互作用能，证实了该传感器对3-MCPD具有强亲和力和高选择性。在ECL体系中，3-MCPD有效猝灭了[Ru（bpy）₃]2+的发光，表明其在传感机制中具有很强的相互作用。该传感器检测限为21.68 nM，灵敏度为0.22 a.u. nM−1 mm−2，具有较高的3-MCPD检测性能。通过与MIP方法的结合，实现了高ECL选择性，增强了靶分子识别，为设计用于食品污染物监测的鲁棒传感器提供了新的途径。此外，计算预测与实验验证的结合为设计选择性和灵敏度高的检测平台提供了可靠的策略。该传感器成功应用于食用油基质中的3-MCPD检测，证明了其在受控实验室条件下对加标油基质的适用性。

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引用次数: 0

DNA-based nanomaterials for fluorescence imaging of tumor-associated biomolecules 用于肿瘤相关生物分子荧光成像的dna纳米材料

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2026-01-01 DOI: 10.1016/j.microc.2025.116723

Wenjun Zhu , Yubai Liu , Longyu Zhao , Han Wu , Guohua Wang , Zhe Dong

Cancer is one of the major causes of death worldwide, and the early and precise detection is critical for improving the survival level of patients. Traditional imaging techniques are often inadequate due to the limitations in sensitivity, cost, and potential radiation damage. Fluorescence imaging, because of its high sensitivity, low cost, and high spatial resolution, has been widely used for the monitoring of various cancer biomarkers. Nucleic acid molecules, particularly deoxyribonucleic acid (DNA), can serve as versatile building blocks for constructing nanomaterials due to their programmability, biocompatibility, and ability to encode biological information. The integration of DNA with nanomaterials confers unique advantages such as enhanced stability, targeted delivery, and functional diversity, making them ideal for the recognition and detection of multiple tumor-related biomarkers. The DNA-based nanomaterials can be applied in cancer diagnosis, drug delivery, and bioimaging, offering new strategies for early illness detection and tailored therapeutics. This review provides a comprehensive overview of recent advancements in DNA-based nanomaterials for the fluorescence imaging of tumor-associated biomolecules. We discuss the design principles and applications of various DNA-based nanomaterials, highlighting the potential advancements in bioimaging research and cancer diagnosis. This review aims to give a summary of the development and applications in DNA-based nanomaterials in tumor-associated fluorescence imaging of biomarkers, and gives the direction of future investigation in the development of this field to help the progress of real-time tumor diagnosis and early theranostics.

癌症是世界范围内的主要死亡原因之一，早期准确的发现对提高患者的生存水平至关重要。由于灵敏度、成本和潜在的辐射损伤的限制，传统的成像技术往往是不充分的。荧光成像以其高灵敏度、低成本、高空间分辨率等优点，被广泛应用于各种癌症生物标志物的监测。核酸分子，特别是脱氧核糖核酸（DNA），由于其可编程性、生物相容性和编码生物信息的能力，可以作为构建纳米材料的通用构建块。DNA与纳米材料的整合具有独特的优势，如增强的稳定性，靶向递送和功能多样性，使其成为识别和检测多种肿瘤相关生物标志物的理想选择。基于dna的纳米材料可以应用于癌症诊断、药物传递和生物成像，为早期疾病检测和定制治疗提供了新的策略。本文综述了用于肿瘤相关生物分子荧光成像的dna纳米材料的最新进展。我们讨论了各种基于dna的纳米材料的设计原理和应用，强调了生物成像研究和癌症诊断的潜在进展。本文综述了基于dna的纳米材料在肿瘤相关生物标志物荧光成像中的研究进展和应用，并提出了该领域未来的研究方向，以帮助肿瘤实时诊断和早期治疗取得进展。

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