Microchemical Journal最新文献_第9页

RAA-CRISPR/Cas13a-assisted lateral flow strip for Feline herpesvirus Type I point-of-care testing

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2025-02-22 DOI: 10.1016/j.microc.2025.113135

Qin Wu , Gan Lin , Junfeng Li , Binjie Zhu , Chenxi Lei , Zheqi Zhou , Yuhe Fu , Lei Lei , Jiali Xu , Jing Xia , Lingli Jiang , Houhui Song , Changyong Cheng

Feline herpesvirus type 1 (FHV-1) is the major pathogen of feline upper respiratory tract disease (URTD) and ocular disease, accounting for approximately 50 %–75 % of URTD in susceptible cats. However, similar clinical symptoms of URTD were present in the early stages of URTD, which makes it difficult to determine the pathogens with the naked eye. For that, there are a variety of nucleic acid detection methods have been established for FHV-1 determination in past years. However, they need complicated thermal cycling, specialized instruments, long detection time, brings false results risk and etc, which is not suitable for point-of-care testing. Therefore, this study developed a new nucleic acid detection method depending on recombinase aided amplification (RAA) (37 °C, 25 min), CRISPR/Cas13a trans-cleavage (37 °C, 20 min) and lateral flow strip (room temperature, 3 min). Firstly, the recombinant plasmid DNA, crRNA, and RAA primer pairs targeting the FHV-1 UL23 gene were designed and prepared. Secondly, the nucleic acid was extracted by lysis buffer and simple centrifugation. Thirdly, FHV-1 RAA nucleic acid amplification reaction was performed followed by CRISPR/Cas13a trans-cleavage report RNA and lateral flow strip visual detection. As a result, the present assay showed high specificity towards FHV-1 without cross-reactivity with other pathogens resulted in URTD and high sensitivity with the limit of detection at 0.768 copies/μL for the FHV-1 UL23 gene. Furthermore, clinical samples application was conducted for 24 clinical swab samples collected from cats with URTD using the present assay and the reference fluorescent quantitative PCR method simultaneously. As we expected, the results of the present assay were in exceptional concordance with the reference fluorescent quantitative PCR method. Accordingly, the present RAA-CRISPR/Cas13a-assisted lateral flow strip was developed for the rapid, ultra-sensitive, simple-operation, relible, visual and point-of-care testing of FHV-1, which ensures FHV-1’s rapid diagnosis in the early stages of infection and support a appropriate treatment on time in resource-limited field.

{"title":"RAA-CRISPR/Cas13a-assisted lateral flow strip for Feline herpesvirus Type I point-of-care testing","authors":"Qin Wu , Gan Lin , Junfeng Li , Binjie Zhu , Chenxi Lei , Zheqi Zhou , Yuhe Fu , Lei Lei , Jiali Xu , Jing Xia , Lingli Jiang , Houhui Song , Changyong Cheng","doi":"10.1016/j.microc.2025.113135","DOIUrl":"10.1016/j.microc.2025.113135","url":null,"abstract":"<div><div>Feline herpesvirus type 1 (FHV-1) is the major pathogen of feline upper respiratory tract disease (URTD) and ocular disease, accounting for approximately 50 %–75 % of URTD in susceptible cats. However, similar clinical symptoms of URTD were present in the early stages of URTD, which makes it difficult to determine the pathogens with the naked eye. For that, there are a variety of nucleic acid detection methods have been established for FHV-1 determination in past years. However, they need complicated thermal cycling, specialized instruments, long detection time, brings false results risk and etc, which is not suitable for point-of-care testing. Therefore, this study developed a new nucleic acid detection method depending on recombinase aided amplification (RAA) (37 °C, 25 min), CRISPR/Cas13a <em>trans</em>-cleavage (37 °C, 20 min) and lateral flow strip (room temperature, 3 min). Firstly, the recombinant plasmid DNA, crRNA, and RAA primer pairs targeting the FHV-1 UL23 gene were designed and prepared. Secondly, the nucleic acid was extracted by lysis buffer and simple centrifugation. Thirdly, FHV-1 RAA nucleic acid amplification reaction was performed followed by CRISPR/Cas13a <em>trans</em>-cleavage report RNA and lateral flow strip visual detection. As a result, the present assay showed high specificity towards FHV-1 without cross-reactivity with other pathogens resulted in URTD and high sensitivity with the limit of detection at 0.768 copies/μL for the FHV-1 UL23 gene. Furthermore, clinical samples application was conducted for 24 clinical swab samples collected from cats with URTD using the present assay and the reference fluorescent quantitative PCR method simultaneously. As we expected, the results of the present assay were in exceptional concordance with the reference fluorescent quantitative PCR method. Accordingly, the present RAA-CRISPR/Cas13a-assisted lateral flow strip was developed for the rapid, ultra-sensitive, simple-operation, relible, visual and point-of-care testing of FHV-1, which ensures FHV-1’s rapid diagnosis in the early stages of infection and support a appropriate treatment on time in resource-limited field.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"211 ","pages":"Article 113135"},"PeriodicalIF":4.9,"publicationDate":"2025-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143487436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Circulating tumour cells isolation in Asymmetrical meandering microchannel

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2025-02-22 DOI: 10.1016/j.microc.2025.113132

Ahmed A. Ayash

A microfluidic method is presented in the current work which allows full separation of circulating tumour cells (CTCs) from the blood biopsy of cancer patients. The method is based on using a microchannel with two separation zones. The first zone is meandering and equipped with seven identical electrodes. These electrodes generate dielectrophoretic forces that affect mostly the platelets and force them to separate first in this zone. This leaves only the target cells (CTCs) with RBCs and WBCs to flow through an expanded, curved section of the channel (second zone). This unique configuration allows generating intense hydrodynamic forces that can isolate CTCs completely. A numerical model is used to give insight into the cells separation and examine the performance of the proposed channel at different operating conditions. The computational results indicated that all CTCs can be separated when operating at a blood volume flow rate ranging from 10 to 20 µl/h. The two main electrical parameters (electrode voltage and AC current frequency) are also examined. The results showed that the voltage between 15 and 30 V and frequency between 20 and 70 kHz are the best ranges to isolate pure CTCs completely without including any blood cells (i.e. 100 % both separation efficiency and purity). Further, CTCs size is changed from 12 to 26 µm to mimic the effect of different CTC types and the results demonstrate clearly that with a single design various types of cancerous cells can be handled effectively.

{"title":"Circulating tumour cells isolation in Asymmetrical meandering microchannel","authors":"Ahmed A. Ayash","doi":"10.1016/j.microc.2025.113132","DOIUrl":"10.1016/j.microc.2025.113132","url":null,"abstract":"<div><div>A microfluidic method is presented in the current work which allows full separation of circulating tumour cells (CTCs) from the blood biopsy of cancer patients. The method is based on using a microchannel with two separation zones. The first zone is meandering and equipped with seven identical electrodes. These electrodes generate dielectrophoretic forces that affect mostly the platelets and force them to separate first in this zone. This leaves only the target cells (CTCs) with RBCs and WBCs to flow through an expanded, curved section of the channel (second zone). This unique configuration allows generating intense hydrodynamic forces that can isolate CTCs completely. A numerical model is used to give insight into the cells separation and examine the performance of the proposed channel at different operating conditions. The computational results indicated that all CTCs can be separated when operating at a blood volume flow rate ranging from 10 to 20 µl/h. The two main electrical parameters (electrode voltage and AC current frequency) are also examined. The results showed that the voltage between 15 and 30 V and frequency between 20 and 70 kHz are the best ranges to isolate pure CTCs completely without including any blood cells (i.e. 100 % both separation efficiency and purity). Further, CTCs size is changed from 12 to 26 µm to mimic the effect of different CTC types and the results demonstrate clearly that with a single design various types of cancerous cells can be handled effectively.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"211 ","pages":"Article 113132"},"PeriodicalIF":4.9,"publicationDate":"2025-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143488025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A study on the dynamic changes and relationships of volatile and semi-volatile compounds in flaxseed during the roasting procedure by using untargeted GC–MS combined with advanced chemometrics

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2025-02-22 DOI: 10.1016/j.microc.2025.113130

Lu Han , Long-He Wang , Yan-Jin Wen , Xue-Juan Zhang , Ping-Ping Liu , Xiang-Dong Zhao , Qing-Xia Zheng , Guo-Bi Chai , Qi-Dong Zhang , Yong-Jie Yu , Yuanbin She

Roasting flaxseed is an important stage in producing rich aromas. At present, characterizing the dynamically changed compounds is difficult. Here, a novel untargeted strategy for accurately and comprehensively analyzing volatile compounds such as flavors and semi-volatile compounds such as sugars and amino acids was provided, which is accomplished by integrating gas chromatography–mass spectrometry (GC–MS) with our newly developed automatic data analysis software AntDAS-GCMS. Samples at different roasting stages were collected, with the online released volatile compounds and inherent semi-volatile compounds analyzed simultaneously. Raw GC–MS data files were imported into AntDAS-GCMS to automatically perform compound resolution, registration, and identification. Finally, 53 volatile compounds and 83 semi-volatile compounds were screened and identified, whose dynamic variations during roasting were revealed. The relationship between semi-volatile and volatile compounds was studied in detail. The developed strategy provided a new solution for dynamic quality control during roasting.

{"title":"A study on the dynamic changes and relationships of volatile and semi-volatile compounds in flaxseed during the roasting procedure by using untargeted GC–MS combined with advanced chemometrics","authors":"Lu Han , Long-He Wang , Yan-Jin Wen , Xue-Juan Zhang , Ping-Ping Liu , Xiang-Dong Zhao , Qing-Xia Zheng , Guo-Bi Chai , Qi-Dong Zhang , Yong-Jie Yu , Yuanbin She","doi":"10.1016/j.microc.2025.113130","DOIUrl":"10.1016/j.microc.2025.113130","url":null,"abstract":"<div><div>Roasting flaxseed is an important stage in producing rich aromas. At present, characterizing the dynamically changed compounds is difficult. Here, a novel untargeted strategy for accurately and comprehensively analyzing volatile compounds such as flavors and semi-volatile compounds such as sugars and amino acids was provided, which is accomplished by integrating gas chromatography–mass spectrometry (GC–MS) with our newly developed automatic data analysis software AntDAS-GCMS. Samples at different roasting stages were collected, with the online released volatile compounds and inherent semi-volatile compounds analyzed simultaneously. Raw GC–MS data files were imported into AntDAS-GCMS to automatically perform compound resolution, registration, and identification. Finally, 53 volatile compounds and 83 semi-volatile compounds were screened and identified, whose dynamic variations during roasting were revealed. The relationship between semi-volatile and volatile compounds was studied in detail. The developed strategy provided a new solution for dynamic quality control during roasting.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"211 ","pages":"Article 113130"},"PeriodicalIF":4.9,"publicationDate":"2025-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143488024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Research on identification of common bean seed vigor based on hyperspectral and deep learning

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2025-02-22 DOI: 10.1016/j.microc.2025.113133

Shujia Li , Laijun Sun , Xiuliang Jin , Guojun Feng , Lingyu Zhang , Hongyi Bai

Accurate, rapid and non-destructive identification of common bean seed vigor is of great significance for the planting and efficient utilization of common bean. In this study, five common bean varieties were used as research objects, and four samples with different aging levels were obtained through artificial accelerated aging. Based on the standard germination experiment, the difference in vigor between aged seeds and healthy seeds was verified. Hyperspectral data with aging time of 0d, 2d, 4d and 6d were collected respectively, and one-dimensional average spectra were extracted as modeling datasets using image processing technology. Aiming at the problem of rapid identification of common bean seed vigor, a Multi-scale Spectral Attention Residual Network (MSARN) was proposed in this study. VGG19, MoblieNet, Support Vector Machine (SVM), Random Forest (RF), K-Nearest Neighbor (KNN), and Partial Least Squares Discriminant Analysis (PLS-DA) were used to compare the performance. The results showed that compared to the traditional machine learning models, the deep learning models had better identification results without preprocessing, and MSARN had the best performance. After using the twice Successive Projections Algorithm (SPA), 40 characteristic wavelengths were extracted. The accuracy, precision, recall, and f1-score of SPA-SPA-MSARN for identifying common bean seeds of different vigor levels reached 98.75%, 98.97%, 98.80%, and 98.81%, respectively. Finally, the study applied SPA-SPA-MSARN to five single-variety common bean datasets, and the model was tested to achieve 100% accuracy in identifying vigor levels for four of the variety datasets. This study shows that hyperspectral technology combined with deep learning has great potential in identifying common bean seed vigor.

{"title":"Research on identification of common bean seed vigor based on hyperspectral and deep learning","authors":"Shujia Li , Laijun Sun , Xiuliang Jin , Guojun Feng , Lingyu Zhang , Hongyi Bai","doi":"10.1016/j.microc.2025.113133","DOIUrl":"10.1016/j.microc.2025.113133","url":null,"abstract":"<div><div>Accurate, rapid and non-destructive identification of common bean seed vigor is of great significance for the planting and efficient utilization of common bean. In this study, five common bean varieties were used as research objects, and four samples with different aging levels were obtained through artificial accelerated aging. Based on the standard germination experiment, the difference in vigor between aged seeds and healthy seeds was verified. Hyperspectral data with aging time of 0d, 2d, 4d and 6d were collected respectively, and one-dimensional average spectra were extracted as modeling datasets using image processing technology. Aiming at the problem of rapid identification of common bean seed vigor, a Multi-scale Spectral Attention Residual Network (MSARN) was proposed in this study. VGG19, MoblieNet, Support Vector Machine (SVM), Random Forest (RF), K-Nearest Neighbor (KNN), and Partial Least Squares Discriminant Analysis (PLS-DA) were used to compare the performance. The results showed that compared to the traditional machine learning models, the deep learning models had better identification results without preprocessing, and MSARN had the best performance. After using the twice Successive Projections Algorithm (SPA), 40 characteristic wavelengths were extracted. The accuracy, precision, recall, and f1-score of SPA-SPA-MSARN for identifying common bean seeds of different vigor levels reached 98.75%, 98.97%, 98.80%, and 98.81%, respectively. Finally, the study applied SPA-SPA-MSARN to five single-variety common bean datasets, and the model was tested to achieve 100% accuracy in identifying vigor levels for four of the variety datasets. This study shows that hyperspectral technology combined with deep learning has great potential in identifying common bean seed vigor.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"211 ","pages":"Article 113133"},"PeriodicalIF":4.9,"publicationDate":"2025-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143487431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A rapid and green analytical strategy to determine menin inhibitor revumenib in human blood serum via CE-DAD

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2025-02-22 DOI: 10.1016/j.microc.2025.113119

Sergio Fernández-Trujillo , Gregorio Castañeda-Peñalvo , Juana Rodríguez-Flores , Rosa del Carmen Rodríguez Martín-Doimeadios

Revumenib (formerly SNDX-5613) is a novel, potent and oral menin inhibitor employed in clinical settings because of treating acute myeloid leukemia in critically ill patients. Nevertheless, safety is still a matter of concern due to the optimal timing of administration and doses of this anticancer agent. An effective tool that allows to solve the drug dosage regimen is therapeutic drug monitoring. In that sense, simple, sensitive and accurate bioanalytical strategies are required. Here, a rapid capillary electrophoresis with diode-array detector methodology was developed and applied for the first time to detect, identify and determine revumenib in complex clinical matrices such as human blood serum in less than 5 min of analysis with a minimal sample preparation procedure based on a protein precipitation with acetonitrile. Various chemical and instrumental critical parameters were rigorously optimized for the separation process in a fused-silica capillary with a background electrolyte of phosphate buffer (50 mM, pH = 2.0) in deionized water. Adequate analytical performance parameters were obtained involving a good linearity, detection and quantification limits at ng mL⁻¹ levels, and a correct precision and accuracy. Furthermore, this approach can be characterized as an ecofriendly strategy in the bioanalysis field based on an estimated greenness evaluation via AGREEprep and AGREE metrics tool with acceptable scores. Considering the results, the present work can be used as a new medical tool and successfully applied in the therapeutic monitoring of revumenib in routine clinical laboratories but also to take appropriate decisions in future (pre)clinical trials and/or phases.

{"title":"A rapid and green analytical strategy to determine menin inhibitor revumenib in human blood serum via CE-DAD","authors":"Sergio Fernández-Trujillo , Gregorio Castañeda-Peñalvo , Juana Rodríguez-Flores , Rosa del Carmen Rodríguez Martín-Doimeadios","doi":"10.1016/j.microc.2025.113119","DOIUrl":"10.1016/j.microc.2025.113119","url":null,"abstract":"<div><div>Revumenib (formerly SNDX-5613) is a novel, potent and oral menin inhibitor employed in clinical settings because of treating acute myeloid leukemia in critically ill patients. Nevertheless, safety is still a matter of concern due to the optimal timing of administration and doses of this anticancer agent. An effective tool that allows to solve the drug dosage regimen is therapeutic drug monitoring. In that sense, simple, sensitive and accurate bioanalytical strategies are required. Here, a rapid capillary electrophoresis with diode-array detector methodology was developed and applied for the first time to detect, identify and determine revumenib in complex clinical matrices such as human blood serum in less than 5 min of analysis with a minimal sample preparation procedure based on a protein precipitation with acetonitrile. Various chemical and instrumental critical parameters were rigorously optimized for the separation process in a fused-silica capillary with a background electrolyte of phosphate buffer (50 mM, pH = 2.0) in deionized water. Adequate analytical performance parameters were obtained involving a good linearity, detection and quantification limits at ng mL<sup>−1</sup> levels, and a correct precision and accuracy. Furthermore, this approach can be characterized as an ecofriendly strategy in the bioanalysis field based on an estimated greenness evaluation via AGREEprep and AGREE metrics tool with acceptable scores. Considering the results, the present work can be used as a new medical tool and successfully applied in the therapeutic monitoring of revumenib in routine clinical laboratories but also to take appropriate decisions in future (pre)clinical trials and/or phases.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"211 ","pages":"Article 113119"},"PeriodicalIF":4.9,"publicationDate":"2025-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143509974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A novel glucose sensor based on the Cu2O/RGO decorated SWCNT buckypaper as a flexible electrode

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2025-02-22 DOI: 10.1016/j.microc.2025.113125

Hailin Shen , Yutao Shi , Pinyou Zhao , Huaren Wu , Yingjun Chen , Wei Yang , Tianxiang Zhu , Wei Zhang , Xiaohui Chen

With the rapid development of biosensing technology, there is an increasing demand in the market for electrochemical sensors that offer high sensitivity, selectivity, and good biocompatibility. Flexible electrochemical biosensors based on functionalized single-walled carbon nanotubes (SWCNTs) can achieve efficient and rapid detection of glucose in the human body. Although SWCNT films possess excellent conductivity, thermochemical stability, and flexibility. However, there are still shortcomings in catalytic activity and selectivity when applied to glucose electrochemical detection. To address this, we used graphene oxide as an intermediary between SWCNTs and Cu₂O nanoparticles to prepare Cu₂O@RGO modified SWCNTs films (Cu₂O@RGO-SWCNTs). The mixed valence state of Cu₂O and its significant double-layer capacitance effect enable it to exhibit higher activity and efficiency in glucose electrocatalytic reactions. We conducted an in-depth study of the electrochemical properties of Cu₂O@RGO-SWCNTs flexible materials and performed interference tests. Experimental results demonstrate that this electrode exhibits high electrochemical response sensitivity to glucose and a wide linear detection range, showing potential application value in the field of flexible electrochemical sensors. Under optimized conditions, the sensor exhibits a sensitivity of 193.2 μA mM⁻¹ cm⁻² for detecting low concentrations of glucose and maintains the same sensitivity of 149.2 μA mM⁻¹ cm⁻² for high concentrations of glucose. The linear range of the sensor is from 0 to 20 mM.

{"title":"A novel glucose sensor based on the Cu2O/RGO decorated SWCNT buckypaper as a flexible electrode","authors":"Hailin Shen , Yutao Shi , Pinyou Zhao , Huaren Wu , Yingjun Chen , Wei Yang , Tianxiang Zhu , Wei Zhang , Xiaohui Chen","doi":"10.1016/j.microc.2025.113125","DOIUrl":"10.1016/j.microc.2025.113125","url":null,"abstract":"<div><div>With the rapid development of biosensing technology, there is an increasing demand in the market for electrochemical sensors that offer high sensitivity, selectivity, and good biocompatibility. Flexible electrochemical biosensors based on functionalized single-walled carbon nanotubes (SWCNTs) can achieve efficient and rapid detection of glucose in the human body. Although SWCNT films possess excellent conductivity, thermochemical stability, and flexibility. However, there are still shortcomings in catalytic activity and selectivity when applied to glucose electrochemical detection. To address this, we used graphene oxide as an intermediary between SWCNTs and Cu<sub>2</sub>O nanoparticles to prepare Cu<sub>2</sub>O@RGO modified SWCNTs films (Cu<sub>2</sub>O@RGO-SWCNTs). The mixed valence state of Cu<sub>2</sub>O and its significant double-layer capacitance effect enable it to exhibit higher activity and efficiency in glucose electrocatalytic reactions. We conducted an in-depth study of the electrochemical properties of Cu<sub>2</sub>O@RGO-SWCNTs flexible materials and performed interference tests. Experimental results demonstrate that this electrode exhibits high electrochemical response sensitivity to glucose and a wide linear detection range, showing potential application value in the field of flexible electrochemical sensors. Under optimized conditions, the sensor exhibits a sensitivity of 193.2 μA mM<sup>−1</sup> cm<sup>−2</sup> for detecting low concentrations of glucose and maintains the same sensitivity of 149.2 μA mM<sup>−1</sup> cm<sup>−2</sup> for high concentrations of glucose. The linear range of the sensor is from 0 to 20 mM.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"211 ","pages":"Article 113125"},"PeriodicalIF":4.9,"publicationDate":"2025-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143510089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High selectivity, ultra-sensitivity, and ultra-distinguishability of electrochemical sensing platform for simultaneous detection of dopamine, uric acid, and ascorbic acid

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2025-02-22 DOI: 10.1016/j.microc.2025.113123

Xin Xu, Ximing Guo

Dopamine (DA), uric acid (UA), and ascorbic acid (AA), three similar electroactive biological small molecules, generally coexist in human serum, causing serious interference when detected separately. In order to realize the simultaneous detection of DA, UA, and AA, three similar electroactive biomolecules, through the electrochemical sensing platform based on 2D Cu-TCPP MOFs nanofilms, DA molecules were successfully detected and optimized the detection conditions according to our previous detection methods for UA and AA. The results displayed the perfect electrochemical response signal of DA, excellent linear correlation between current intensity and DA concentration, LODs, repeatability, and stability. The electrochemical potential (E_pa) of UA oxidation peak was intermediate between E_pa of AA and DA oxidation peak, the response current intensity of UA oxidation peak continuously decreases with increases of UA concentration. The response current intensity of DA and AA oxidation peak on both sides of UA gradually increases with the increase of DA and AA concentration. On the other hand, there is a significant difference in the oxidation potential difference between adjacent substances DA, UA, and AA (ΔE_AA-UA = 141 mV, ΔE_UA-DA = 167 mV, and ΔE_AA-DA = 308 mV), which will play a decisive role in the simultaneous detection of DA, UA, and AA based on 2D Cu-TCPP MOFs/GCE electrochemical sensing platform. Therefore, the electrochemical platform based on 2D Cu-TCPP MOFs nanofilms is more conducive to the simultaneous detection of DA, UA, and AA with high selectivity, ultra-sensitivity, and ultra-distinguishability.

{"title":"High selectivity, ultra-sensitivity, and ultra-distinguishability of electrochemical sensing platform for simultaneous detection of dopamine, uric acid, and ascorbic acid","authors":"Xin Xu, Ximing Guo","doi":"10.1016/j.microc.2025.113123","DOIUrl":"10.1016/j.microc.2025.113123","url":null,"abstract":"<div><div>Dopamine (DA), uric acid (UA), and ascorbic acid (AA), three similar electroactive biological small molecules, generally coexist in human serum, causing serious interference when detected separately. In order to realize the simultaneous detection of DA, UA, and AA, three similar electroactive biomolecules, through the electrochemical sensing platform based on 2D Cu-TCPP MOFs nanofilms, DA molecules were successfully detected and optimized the detection conditions according to our previous detection methods for UA and AA. The results displayed the perfect electrochemical response signal of DA, excellent linear correlation between current intensity and DA concentration, LODs, repeatability, and stability. The electrochemical potential (E<sub>pa</sub>) of UA oxidation peak was intermediate between E<sub>pa</sub> of AA and DA oxidation peak, the response current intensity of UA oxidation peak continuously decreases with increases of UA concentration. The response current intensity of DA and AA oxidation peak on both sides of UA gradually increases with the increase of DA and AA concentration. On the other hand, there is a significant difference in the oxidation potential difference between adjacent substances DA, UA, and AA (ΔE<sub>AA-UA</sub> = 141 mV, ΔE<sub>UA-DA</sub> = 167 mV, and ΔE<sub>AA-DA</sub> = 308 mV), which will play a decisive role in the simultaneous detection of DA, UA, and AA based on 2D Cu-TCPP MOFs/GCE electrochemical sensing platform. Therefore, the electrochemical platform based on 2D Cu-TCPP MOFs nanofilms is more conducive to the simultaneous detection of DA, UA, and AA with high selectivity, ultra-sensitivity, and ultra-distinguishability.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"212 ","pages":"Article 113123"},"PeriodicalIF":4.9,"publicationDate":"2025-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143534155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detection of Ca2+ in water samples and calcium gluconate oral liquids using gas pressure meter as portable detector

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2025-02-22 DOI: 10.1016/j.microc.2025.113116

Dali Zhuo , Xiaoyu Dong , Linghuan Li , Ronghua Zeng , Chao Tan , Hong Luo , Zhirong Zou

Calcium ion (Ca²⁺) is one of the important nutrients for humans, excessive and insufficient intake may both cause diseases, so that it is of great importance to analysis of Ca²⁺ in our daily life. In this work, a portable gas pressure meter-based method was developed for the detection of Ca²⁺ in water samples and calcium gluconate oral liquids. Ca²⁺ in solution was extracted and precipitated by the addition of Na₂C₂O₄, CaC₂O₄ precipitate was formed and then filtered from the complicated matrix through a filter membrane. The filter membrane (containing CaC₂O₄ precipitate) was then treated by H₂SO₄ solution, CaC₂O₄ precipitate was dissolved during this step and H₂C₂O₄ was formed. In a sealed bottle-in-bottle reaction device, H₂C₂O₄ reacted with acidic KMnO₄ solution to generate CO₂ gas, causing a significant gas pressure increase and it was readout by a hand-held pressure meter. A standard curve with a linear range of 1–80 mmol L⁻¹ and a limit of detection of 0.4 mmol L⁻¹ were obtained. This system was subsequently applied to the detection of Ca²⁺ in several water samples and oral liquids, recoveries of 92–110% were obtained for their spiked samples. Only a few reaction bottles and a gas pressure meter were required in this system. It is a low-cost, sensitive, rapid and easy-used analytical method for on-site determination of Ca²⁺.

{"title":"Detection of Ca2+ in water samples and calcium gluconate oral liquids using gas pressure meter as portable detector","authors":"Dali Zhuo , Xiaoyu Dong , Linghuan Li , Ronghua Zeng , Chao Tan , Hong Luo , Zhirong Zou","doi":"10.1016/j.microc.2025.113116","DOIUrl":"10.1016/j.microc.2025.113116","url":null,"abstract":"<div><div>Calcium ion (Ca<sup>2+</sup>) is one of the important nutrients for humans, excessive and insufficient intake may both cause diseases, so that it is of great importance to analysis of Ca<sup>2+</sup> in our daily life. In this work, a portable gas pressure meter-based method was developed for the detection of Ca<sup>2+</sup> in water samples and calcium gluconate oral liquids. Ca<sup>2+</sup> in solution was extracted and precipitated by the addition of Na<sub>2</sub>C<sub>2</sub>O<sub>4</sub>, CaC<sub>2</sub>O<sub>4</sub> precipitate was formed and then filtered from the complicated matrix through a filter membrane. The filter membrane (containing CaC<sub>2</sub>O<sub>4</sub> precipitate) was then treated by H<sub>2</sub>SO<sub>4</sub> solution, CaC<sub>2</sub>O<sub>4</sub> precipitate was dissolved during this step and H<sub>2</sub>C<sub>2</sub>O<sub>4</sub> was formed. In a sealed bottle-in-bottle reaction device, H<sub>2</sub>C<sub>2</sub>O<sub>4</sub> reacted with acidic KMnO<sub>4</sub> solution to generate CO<sub>2</sub> gas, causing a significant gas pressure increase and it was readout by a hand-held pressure meter. A standard curve with a linear range of 1–80 mmol L<sup>−1</sup> and a limit of detection of 0.4 mmol L<sup>−1</sup> were obtained. This system was subsequently applied to the detection of Ca<sup>2+</sup> in several water samples and oral liquids, recoveries of 92–110% were obtained for their spiked samples. Only a few reaction bottles and a gas pressure meter were required in this system. It is a low-cost, sensitive, rapid and easy-used analytical method for on-site determination of Ca<sup>2+</sup>.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"211 ","pages":"Article 113116"},"PeriodicalIF":4.9,"publicationDate":"2025-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143509975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Current trends and advances in nanozyme-enabled miRNA (bio)sensing: Classification, activity and application

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2025-02-22 DOI: 10.1016/j.microc.2025.113127

Esmaeil Babaei , Hewa Jalal Azeez , Shukur Wasman Smail , Jabar Kamal Mirza Abdalla , Muhammed Kasim Diril , Mansour Mahmoudpour , Zahra Karimzadeh

MicroRNAs (miRNAs) play a prominent role in the regulation of post-transcriptional gene expression and are extensively involved in various cellular and developmental processes. Dysregulation of miRNA levels can disrupt normal gene regulation, potentially leading to a variety of human diseases including cancer. Thus, it is of paramount importance to develop accurate, rapid, and sensitive determination techniques for miRNA analysis. Because of their more robust activity, simpler preparation procedures, lower cost, higher stability, and higher recycling efficiency in comparison with that of natural enzymes, nanozymes have recently emerged as a favorable substitute for natural enzymes for the development of biosensors designed for the analysis of miRNAs. Driven by this, significant attempts have been dedicated to the advancement of nanozyme-enabled miRNA biosensors. This review provides a thorough summary and analysis of the catalytic mechanisms, structural classifications, and functions of nanozymes in the development of biosensors. This paper emphatically presents the practical applications of nanozyme-enabled biosensors, emphasizing their advantages in terms of on-site detection capabilities, ease of operation, and cost-effectiveness, including both electrochemical and optical biosensors. Ultimately, the primary challenges and prospective future trends are examined, which may offer theoretical insights for the application of nanozyme-based biosensors in miRNA detection.

{"title":"Current trends and advances in nanozyme-enabled miRNA (bio)sensing: Classification, activity and application","authors":"Esmaeil Babaei , Hewa Jalal Azeez , Shukur Wasman Smail , Jabar Kamal Mirza Abdalla , Muhammed Kasim Diril , Mansour Mahmoudpour , Zahra Karimzadeh","doi":"10.1016/j.microc.2025.113127","DOIUrl":"10.1016/j.microc.2025.113127","url":null,"abstract":"<div><div>MicroRNAs (miRNAs) play a prominent role in the regulation of post-transcriptional gene expression and are extensively involved in various cellular and developmental processes. Dysregulation of miRNA levels can disrupt normal gene regulation, potentially leading to a variety of human diseases including cancer. Thus, it is of paramount importance to develop accurate, rapid, and sensitive determination techniques for miRNA analysis. Because of their more robust activity, simpler preparation procedures, lower cost, higher stability, and higher recycling efficiency in comparison with that of natural enzymes, nanozymes have recently emerged as a favorable substitute for natural enzymes for the development of biosensors designed for the analysis of miRNAs. Driven by this, significant attempts have been dedicated to the advancement of nanozyme-enabled miRNA biosensors. This review provides a thorough summary and analysis of the catalytic mechanisms, structural classifications, and functions of nanozymes in the development of biosensors. This paper emphatically presents the practical applications of nanozyme-enabled biosensors, emphasizing their advantages in terms of on-site detection capabilities, ease of operation, and cost-effectiveness, including both electrochemical and optical biosensors. Ultimately, the primary challenges and prospective future trends are examined, which may offer theoretical insights for the application of nanozyme-based biosensors in miRNA detection.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"211 ","pages":"Article 113127"},"PeriodicalIF":4.9,"publicationDate":"2025-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143511445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Co9S8-electrochemically reduced graphene oxide nanocomposites: A powerful electrochemical sensing platform for DA Co9S8-电化学还原氧化石墨烯纳米复合材料：用于 DA 的强大电化学传感平台

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal

Pub Date : 2025-02-22 DOI: 10.1016/j.microc.2025.113131

Peihong Deng , Nana Tang , Shuting Shi , Chuanqin Zhou , Aiting Chen , Quanguo He

In this study, a preparation of hollow nanoscale tubular polysulfide Co₉S₈ is reported, along with its use in building an electrochemical sensor that can detect dopamine (DA). Utilizing electrochemically reduced graphene oxide (ErGO) as a substrate, the Co₉S₈-ErGO/GCE sensor was made by attaching Co₉S₈ nanotubes to a glassy carbon electrode (GCE) surface. The use of ErGO in the sensor fabrication process not only improves the conductivity but also effectively prevents the aggregation of Co₉S₈. A large number of hollow Co₉S₈ nanotubes are anchored on ErGO, providing more catalytic active sites for electrode reactions. The constructed sensor shows remarkable DA detection ability, with a notable rise in peak current, thanks to the synergistic action of Co₉S₈ and ErGO. This sensor has a lower detection limit of 4.0 nM and a wide linear range of 0.01–0.1 µM, 0.1–2.0 µM, and 2.0–10 µM. It is also possible to quantify DA in actual samples using Co₉S₈-ErGO/GCE, meeting clinical requirements for reproducibility, selectivity and accuracy.

{"title":"Co9S8-electrochemically reduced graphene oxide nanocomposites: A powerful electrochemical sensing platform for DA","authors":"Peihong Deng , Nana Tang , Shuting Shi , Chuanqin Zhou , Aiting Chen , Quanguo He","doi":"10.1016/j.microc.2025.113131","DOIUrl":"10.1016/j.microc.2025.113131","url":null,"abstract":"<div><div>In this study, a preparation of hollow nanoscale tubular polysulfide Co<sub>9</sub>S<sub>8</sub> is reported, along with its use in building an electrochemical sensor that can detect dopamine (DA). Utilizing electrochemically reduced graphene oxide (ErGO) as a substrate, the Co<sub>9</sub>S<sub>8</sub>-ErGO/GCE sensor was made by attaching Co<sub>9</sub>S<sub>8</sub> nanotubes to a glassy carbon electrode (GCE) surface. The use of ErGO in the sensor fabrication process not only improves the conductivity but also effectively prevents the aggregation of Co<sub>9</sub>S<sub>8</sub>. A large number of hollow Co<sub>9</sub>S<sub>8</sub> nanotubes are anchored on ErGO, providing more catalytic active sites for electrode reactions. The constructed sensor shows remarkable DA detection ability, with a notable rise in peak current, thanks to the synergistic action of Co<sub>9</sub>S<sub>8</sub> and ErGO. This sensor has a lower detection limit of 4.0 nM and a wide linear range of 0.01–0.1 µM, 0.1–2.0 µM, and 2.0–10 µM. It is also possible to quantify DA in actual samples using Co<sub>9</sub>S<sub>8</sub>-ErGO/GCE, meeting clinical requirements for reproducibility, selectivity and accuracy.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"211 ","pages":"Article 113131"},"PeriodicalIF":4.9,"publicationDate":"2025-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143487282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0