Microchemical Journal最新文献

{"title":"Real time monitoring of heavy metal adulteration in biodiesel using Arduino UNO platform@A promising multi-purpose stimuli-responsive azomethine based chemoreceptor for hierarchical tri-ionic sensing","authors":"Rajeshwari Pal ,&nbsp;Riyanka Das ,&nbsp;Adwitiya Pal ,&nbsp;Bishwajit Singh Kapoor ,&nbsp;Krishnendu Kundu ,&nbsp;Arunabha Thakur ,&nbsp;Sudit Sekhar Mukhopadhyay ,&nbsp;Priyabrata Banerjee","doi":"10.1016/j.microc.2024.111739","DOIUrl":"10.1016/j.microc.2024.111739","url":null,"abstract":"<div><div>The presence of metallic adulteration even in very trace level may lead to deleterious impact on the biodiesel quality and ultimately may be responsible for the dropping down of system efficiency, which necessitates the trace level recognition of heavy metal adulteration from biodiesel. In this context, herein using an azomethine functionalized chromogenic chemoreceptor, 1-((E)-(4-hydroxyphenylimino) methyl) napthalen-2-ol (<strong>HMN</strong>) has been reported for the selective chemodosimetric recognition of Cu²⁺ with a lower detection threshold of 7.8 ppb <em>via</em> distinct chromogenic variation of <strong>HMN</strong>. In addition to this, it has also exhibited selective and reversible naked eye chromogenic sensing behaviour towards F⁻ (LOD=160 ppb) and Al³⁺ (LOD=48 ppb) at very trace level, which is quite lower than the WHO permissible limit. Distinct chromogenic recognition of F⁻ by <strong>HMN</strong> proceeds <em>via</em> strong intermolecular hydrogen bonding mediated improved intramolecular charge transfer. The spectroscopic response of <strong>HMN</strong> in alternate presence and absence of the targeted analytes made it suitable to formulate AND-NOT-XNOR-NAND-OR gate based ‘<em>Castle-like</em>’ complicated logic circuitry. <em>In-vitro</em> cell imaging study using A549, human lung carcinoma cell line attests intracellular recognition capability of <strong>HMN</strong>, demonstrating its effectual bio-medicinal applications. Interestingly, inspired by the capability of <strong>HMN</strong> towards recognition of copper from biodiesel specimen, an RGB-assisted device comprised of TCS color sensor and an Arduino UNO 8-bit microcontroller has been developed for real-time quantitative analysis of the biodiesel adulteration. Going one step further, utilization of lab-on-a-box based prototype to monitor the concentration-dependent chromogenic fluctuation <em>via</em> RGB analysis is undoubtedly beneficial for the determination of copper adulteration in biodiesel sample. The presently developed chemoreceptor can thus be regarded as a valuable addition in the field of supramolecular chemistry as well as a significant initiative towards regular monitoring of the biodiesel quality control parameters as a part of sustainable environment.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"207 ","pages":"Article 111739"},"PeriodicalIF":4.9,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142322199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Peptide-based fluorescent probe with large Stokes shift for specific sequential detection of copper (II) ions and histidine: Smartphone device, real samples, test strips and bioimaging applications","authors":"Peng Wang ,&nbsp;Xinlin Cao ,&nbsp;Shirui Xue ,&nbsp;Zhijie Wang ,&nbsp;Yi Zhou ,&nbsp;Jiang Wu","doi":"10.1016/j.microc.2024.111728","DOIUrl":"10.1016/j.microc.2024.111728","url":null,"abstract":"<div><div>A novel and facile fluorescent probe, <strong>DNH</strong>, was developed based on a dipeptide backbone (Asn-His-NH₂) labelled with a dansyl fluorophore. <strong>DNH</strong> exhibited significant advantages in detecting Cu²⁺ based on the paramagnetic quenching mechanism, including satisfactory water solubility (100 % aqueous medium), a large Stokes shift (230 nm), excellent anti-interference capability (no interference), a low detection limit (19.6 nM), rapid response time (within 30 s) and a wide pH range (7–12). Employing a displacement approach, the <strong>DNH</strong>-Cu²⁺ ensemble showed strong fluorescence enhancement upon recognition of _L-His with outstanding selectivity, and the detection limit for the <strong>DNH</strong>-Cu²⁺ ensemble with respect to _L-His was calculated at 22.8 nM. Notably, <strong>DNH</strong> demonstrated favourable reversibility for at least seven cycles with minor changes in fluorescence intensity when Cu²⁺ and _L-His were added alternately. Moreover, <strong>DNH</strong> exhibited excellent low cytotoxicity and good biocompatibility, and was utilised as an outstanding probe for the sequential determination of Cu²⁺ and _L-His in living cells and zebrafish. <strong>DNH</strong> was successfully used to determine Cu²⁺ and _L-His in three actual water samples with good recovery and accuracy. In addition, <strong>DNH</strong> was applied to filter paper to develop visual test strips for the rapid and high-efficiency analysis of Cu²⁺ and _L-His. Furthermore, the semi-quantitative visual monitoring of Cu²⁺ and _L-His was achieved by developing a smartphone colour recogniser into a portable analytical device, achieving low detection limits of 0.49 μM and 0.63 μM, respectively. Given these attributes, <strong>DNH</strong> presents itself as a promising peptide-based fluorescent probe, showcasing considerable potential for real-time visual monitoring of Cu²⁺ and _L-His in various environmental and biological settings.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"207 ","pages":"Article 111728"},"PeriodicalIF":4.9,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142310712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A focused review on organic electrochemical transistors: A potential futuristic technological application in microelectronics","authors":"Ahsan Raza ,&nbsp;Umar Farooq ,&nbsp;Khalida Naseem ,&nbsp;Sarfaraz Alam ,&nbsp;Mohammad Ehtisham Khan ,&nbsp;Akbar Mohammad ,&nbsp;Waleed Zakri ,&nbsp;Muhammad Yasir Khan","doi":"10.1016/j.microc.2024.111737","DOIUrl":"10.1016/j.microc.2024.111737","url":null,"abstract":"<div><h3>Background</h3><div>An organic electrochemical transistor (OECT) is an important device in an area of the art in bioelectronics that can convert ionic and biological inputs into electronic outputs with its aqueous environment. The OECTs use a combination of electronic and ionic charges to leverage the mixed conduction properties of materials within their channel. As bioelectronics applications become popular in OECTs, there is a need to standardize the material channel mixed insulation characteristics, however there is some variation in conditions. This review provides an in-depth formulation of OECTs, including their selection mechanisms, use of organic materials, fabrication methods, and applications to bioelectronics devices. Moreover, it offers a critical assessment of OECT’s research and development in the future.</div></div><div><h3>Results</h3><div>The review is organized as follows: First, we provide an overview of the construction and operation of an OECT device in section 2. Next, in section 3, we describe how OECT behavior differs from conventional metal oxide semiconductor field effect transistor (MOSFET), and we discuss how electronic charge transport in the channel by Bernard’s Model. In section 4, we discuss several materials for the transient behavior of OECTs. In section 5, we deal with the recent advances in OECT device fabrication. Then, in section 6, we consider the current applications of OECTs in the fields of biosensing, circuits and logic, neuromorphic computing, and prosthetic and human–machine interfaces. In section 7, we describe limitations concerning the OECT device with expert suggestions to address these limitations. Finally, in section 8, we close the Review with a brief conclusion.</div></div><div><h3>Significance</h3><div>A significant advancement has been made in this field through the development of innovative approaches, such as spray coating for uniform channel deposition and screen printing for large-scale production. The full potential of OECTs in revolutionizing bioelectronics and healthcare applications will be realized only through collaborative efforts between researchers, engineers, and industry stakeholders.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"207 ","pages":"Article 111737"},"PeriodicalIF":4.9,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142317836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"AuNP@Self-assembled 4H-Chromene-Enhanced colorimetric detection of ganciclovir using smartphone Technology: Portable solutions for healthcare","authors":"Geetika Bhardwaj ,&nbsp;Randeep Kaur ,&nbsp;Sanjeev Saini ,&nbsp;Narinder Singh ,&nbsp;Navneet Kaur","doi":"10.1016/j.microc.2024.111734","DOIUrl":"10.1016/j.microc.2024.111734","url":null,"abstract":"<div><div>Any substance, radiation, or radionuclide in our environment that promotes carcinogenesis is a cancerogen. In the current work, we modified the <em>4H</em>-chromene derivative by covering its surface with a shell of gold nanoparticles. Interestingly, ganciclovir (GCV) formed a bridge between the Nap-group of <em>4H</em>-chromene and gold nanoparticles to display surface charge reduction properties in <strong>AuNP@Self-assembled <em>4H</em>-Chromene</strong>. The surface charge of AuNPs is reduced as a result of the electron transfer mechanism brought on by the creation of the Nap-GCV-AuNP link, which leads to agglomeration. This led to the development of a dual-mode colorimetric sensor for GCV that incorporates solution form and portable sensor strip. The probe showed a linear range of 0–74 nM under ideal circumstances, with a detection limit of 4 nM in solution and 3 nM on paper strip detection. An evaluation of the practical utility in human serum using a portable visual read-out produced satisfactory findings, with a recovery percentage of 102.1 % and a standard deviation of ± 4 %. Surprisingly, this is the first work on the colorimetric detection of Ganciclovir that we are aware of.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"207 ","pages":"Article 111734"},"PeriodicalIF":4.9,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142310711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Selective separation of polyphenols with a novel azo porous organic polymer: Ultrahigh adsorption capacity and superfast adsorption rate","authors":"Xian Cheng ,&nbsp;Miao-Miao Long ,&nbsp;Sheng-Nan Li ,&nbsp;Peng-Fei Li ,&nbsp;Pan-Pan Li ,&nbsp;Liang-Wu Bi ,&nbsp;Yu-Xiang Chen","doi":"10.1016/j.microc.2024.111696","DOIUrl":"10.1016/j.microc.2024.111696","url":null,"abstract":"<div><div>For the first time, a novel porous organic polymer (POP), namely Cin-POP, was constructed under normal temperature and pressure using less toxic basic fuchsin and natural cinnamic acid. Cin-POP was synthesized through the diazo-coupling reaction between the diazonium salts of basic fuchsin and cinnamic acid. The FT-IR spectra and XPS analysis prove the successful construction of the Cin-POP, and the BET analysis demonstrates the high-surface-area (166.42–202.56 m² g⁻¹) and suitable pore sizes (11.00–18.63 nm). Owing to the advantages of porous structures, abundant aromatic groups, good thermal stability, and good dispersity, Cin-POP exhibits remarkable adsorption rates and capacities towards polyphenols, including procyanidine and rutin. Especially for procyanidine, the extraction efficiency can arrive at nearly 98.1 % after 2.5 min adsorption, and the pseudo-second-order rate constant (k₂) of Cin-POP is 0.0257 g mg⁻¹ min⁻¹. The maximum adsorption capacities of Cin-POP towards procyanidine and rutin are 2500.00 mg g⁻¹ and 1814.83 mg g⁻¹, outpacing all reported adsorbents. In addition, Cin-POP can selectively adsorb polyphenol among the mixtures of polyphenols and alkaloids. More importantly, Cin-POP can be used for polyphenols separation from complex cinnamon extract. All the above advantages make Cin-POP comparable porous adsorbent for polyphenol separation.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"207 ","pages":"Article 111696"},"PeriodicalIF":4.9,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142310715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hemoglobin-Capped carbon dots synthesized via microwave green approach as a biosensor for specific cholesterol detection","authors":"Nahid Shahabadi ,&nbsp;Kobra Omidfar ,&nbsp;Saba Zendehcheshm","doi":"10.1016/j.microc.2024.111652","DOIUrl":"10.1016/j.microc.2024.111652","url":null,"abstract":"<div><div>This study explores the potential of hemoglobin-capped green-synthesized carbon dots (g-CD) as a biosensor for the specific detection of cholesterol. The CDs, synthesized using an environmentally friendly and convenient microwave green approach, derived from Wood Extract of Wild Cherry Shrub and utilizing hemoglobin as a capping agent, offer promising characteristics for cholesterol detection. This green synthesis method avoids the use of toxic precursors, making the process safer and more sustainable. Additionally, microwave (MW) heating, being volumetric in nature, reduces the synthesis time and energy consumption, resulting in a uniform and unique microstructure, offering a substantial advantage over traditional methods. Through fluorescence spectroscopy, the interaction between the g-CD/Hb complex and cholesterol was investigated. The results demonstrate that the fluorescence intensity of the g-CD/Hb complex increases with the concentration of cholesterol, suggesting a potential application for cholesterol detection. Notably, the calculated limit of detection (LOD) was found to be 9.22 μM, significantly lower than previously reported sensors, indicating higher sensitivity. Moreover, the biosensor exhibits high selectivity towards cholesterol over other biomolecules commonly found in human serum. Additionally, the biosensor shows excellent performance with real human blood samples, indicating its practical utility in clinical settings. Overall, the g-CD/Hb biosensor presents a promising approach for the specific detection of cholesterol, offering potential applications in biomedical research and clinical diagnostics. The key innovations include the use of a green and rapid synthesis method, improved detection limit, and validation of performance in real samples, distinguishing this sensor from previous research.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"207 ","pages":"Article 111652"},"PeriodicalIF":4.9,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142310600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Real-time detection of cerebral edema in mice based on low-field nuclear magnetic resonance","authors":"Yu He ,&nbsp;Xiaoyong Rao ,&nbsp;Yang Zhan ,&nbsp;Qing Tao ,&nbsp;Yan He ,&nbsp;Xiaoming Fu ,&nbsp;Yan Zeng ,&nbsp;Xiaojian Luo ,&nbsp;Haowei Lu ,&nbsp;Wei Liu","doi":"10.1016/j.microc.2024.111738","DOIUrl":"10.1016/j.microc.2024.111738","url":null,"abstract":"<div><div>Cerebral edema is a secondary symptom of several conditions, including stroke, liver, failure, and severe craniocerebral injury. This study aimed to propose a Low-Field Nuclear Magnetic Resonance (LF-NMR) method for rapid and nondestructive measurement of brain water content and water phase state evaluating animal brain edema models. The water transverse relaxation time (<em>T₂</em>) distribution in brain tissues was ascertained by measuring the <em>T₂</em> spectrograms of mouse brain tissues following various drying durations, in accordance with the shifting law of <em>T₂</em> peaks. A methodological study was carried out for the determination of water content in mouse brain tissue by the LF-NMR Peak Area (LF-NMR-PA) method using CuSO₄ solution as standard solution. This method was studied in comparison with the wet/dry weight method and the Partial Least Squares Regression (PLSR) prediction method in normal and MCAO model mice, and further applied to the study of mannitol treatment of cerebral edema to validate the precision and accuracy of the hydration measurements as well as the method’s impact on subsequent experiments of cerebral infarct area measurement. In the <em>T₂</em> spectra of mouse brain tissues, the separate peaks <em>T₂₁</em> (0–10 ms), <em>T₂₂</em> (10–100 ms), and <em>T₂₃</em> (100–1000 ms) correspond to fat and bound water, water, and free water in brain tissue, respectively. The LF-NMR-PA method had good specificity, linearity, precision, stability, reproducibility, and recovery. The accuracy and range of use of the LF-NMR-PA method were better than those of the PLSR method, and its results were not significantly different from those of the wet/dry weight method. However, the short measurement time of the LF-NMR-PA method ensured the integrity of the tissues, and the determination of the moisture by this method did not have a significant effect on the subsequent determination of the area of cerebral infarction, which ensured the consistency of the experimental results.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"207 ","pages":"Article 111738"},"PeriodicalIF":4.9,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142318509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"On-line preconcentration techniques for hydrophobic compounds in capillary electrokinetic chromatography: A review","authors":"Michał Pieckowski,&nbsp;Ilona Olędzka,&nbsp;Tomasz Bączek,&nbsp;Piotr Kowalski","doi":"10.1016/j.microc.2024.111693","DOIUrl":"10.1016/j.microc.2024.111693","url":null,"abstract":"<div><div>Capillary electrophoresis (CE) is a competitive analytical technique to widely used liquid chromatography. It is characterized by low consumption of reagents and organic solvents, as well as relatively short analysis time, which translates into low operating costs and environmental friendliness. Unfortunately, compared to liquid chromatography, the achieved concentration limits of detection (LOD) are significantly higher when using spectrophotometric detection. However, the development of <em>on-line</em> preconcentration techniques has overcome this issue for compounds with hydrophilic characteristics. Nonetheless, for hydrophobic compounds, despite the dynamically evolving separation modes and new pseudostationary phases, there is still a need for new signal enhancement methods. Toxic or pharmacologically active substances present in biological and environmental samples at ultra-trace levels often include hydrophobic compounds, occurring at nanomolar or even picomolar concentrations. Therefore, it is justified to seek new solutions to lower the limits of detection, which will expand knowledge in the field of electromigration techniques for the analysis of hydrophobic compounds. This review summarizes the current achievements in the analysis of hydrophobic compounds using <em>on-line</em> preconcentration techniques in combination with capillary electrokinetic chromatography (CEKC), where separation buffer acts as a pseudostationary phase with which analytes interact.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"207 ","pages":"Article 111693"},"PeriodicalIF":4.9,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142248265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fan assisted extraction and low pressure chromatographic system with a phenyl monolithic column for amperometric determination of volatile α-dicarbonyl compounds in coffee brews","authors":"Alexandra Rangel Silva,&nbsp;Paulo J. Almeida,&nbsp;João Rodrigo Santos","doi":"10.1016/j.microc.2024.111689","DOIUrl":"10.1016/j.microc.2024.111689","url":null,"abstract":"<div><div>In this work, a low pressure chromatographic system featuring a single 0.5 cm length phenyl monolithic column and an amperometric detector was developed to determine volatile ⍺-dicarbonyl compounds in coffee brews. The analytical strategy relied on the extraction of the volatile ⍺-dicarbonyl compounds and their derivatization with <em>o</em>-phenylenediamine, prior to the determination of the resulting quinoxaline derivative compounds in the designed chromatographic system. The studies of the mobile phase composition and electrochemical conditions are presented. The extraction of the volatile ⍺-dicarbonyl compounds was performed resourcing to a recent approach based on the fan assisted extraction system. This system performs the full evaporation technique and allowed the quantitative recovery (&gt;85 %) of diacetyl and 2,3-pentanedione from the coffee brew based on the following experimental conditions: sample volume 30 µL, extraction period 15 min, acceptor solution (water, acetonitrile, 90:10, v/v) volume 400 µL, and sample temperature 50 °C. The developed method showed no matrix effect for different coffee brews and the quantification of the studied analytes can be performed through the external calibration method. Detection limits of 3.6 × 10⁻⁶ mol/L for diacetyl and 12.9 × 10⁻⁶ mol/L for 2,3-pentanedione were obtained. Sample analysis rate was of 5 h⁻¹ and the acetonitrile consumption was of ca. 450 µL per chromatographic run.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"207 ","pages":"Article 111689"},"PeriodicalIF":4.9,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0026265X24018010/pdfft?md5=ff19c16823ef9288f736b4319043eb09&pid=1-s2.0-S0026265X24018010-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142310607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electropolymerized InZnSe@PtAg quantum dots@molecularly imprinted polymer on screen-printed carbon electrodes for the ultrasensitive detection of NS1 dengue virus protein with smartphone-based sensing in saliva","authors":"Kirstie Isla Gray,&nbsp;Oluwasesan Adegoke","doi":"10.1016/j.microc.2024.111704","DOIUrl":"10.1016/j.microc.2024.111704","url":null,"abstract":"<div><div>According to the World Health Organization (WHO), an estimated 100–400 million cases of dengue virus (DENV) infections are recorded annually with half of the global population being at risk of infection. Currently, there is no known treatment for DENV; however, early, rapid and accurate (sensitive and selective) detection can help to alleviate fatality rates. This work reports on the novel development of a point-of-care electrochemical biosensor for DENV using nanostructured InZnSe@PtAg quantum dots (QDs)-molecularly imprinted polymer (MIP) with smartphone-based detection functionality. Highly conductive InZnSe@PtAg QDs were newly synthesized in the presence of metal precursors, organic surfactants and ligands and surface capped with glutathione (GSH) using a ligand exchange reaction. PtAg was used as an electroactive shell layer on the InZnSe QDs core surface to increase the QDs conductivity. The GSH-InZnSe@PtAg QDs were drop-casted onto screen-printed carbon electrodes (SPCEs) and electropolymerized using cyclic voltammetry (CV) in the presence of o-phenylenediamine and the template DENV. The robust electropolymerization process allowed the overcoating of the MIP layer on the QDs/SPCE, where specific DENV size and shape cavities were created. Under optimal experimental conditions, DENV was rapidly, selectively and ultra-sensitively detected. Using differential pulse voltammetry (DPV), quantitative rebinding of DENV on the MIP@QDs/SPCE surface led to a steady decrease of the anodic peak current and a limit of detection of 1.36 pg/mL was obtained for DENV detection. Using a hand-held smartphone-based potentiostat, DENV was successfully detected in human saliva with satisfactory analytic recoveries.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"207 ","pages":"Article 111704"},"PeriodicalIF":4.9,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0026265X24018162/pdfft?md5=c519d407a1fef5f55d8f8c7abe50de9e&pid=1-s2.0-S0026265X24018162-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142310713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}