Pub Date : 1900-01-01DOI: 10.12938/BIFIDUS1982.6.2_55
Charuay Ekataksin, Y. Inagaki, M. Higaki, R. Nakaya
The fecal bacterial flora of three healthy Thai residents in Japan were determined . Two were short-term or long-term vegetarians and one was a mixed-diet omnivore . The total bacterial counts of all these subjects were significantly lower than those of the average Japanese. The total counts and the numbers of anaerobic bacteria in the vegetarians were constant, whereas a fluctuation between the omnivore's two samplings was observed, accompanied with a decrease in bacteroidaceae , eubacteria, bifidobacteria, enterococci, and veillonellae, and an increase in Clostridium perfringens , lactobacilli, and staphylococci.
{"title":"Fecal Flora of Thai Residents in Japan; Comparison between Vegetarians and an Omnivore","authors":"Charuay Ekataksin, Y. Inagaki, M. Higaki, R. Nakaya","doi":"10.12938/BIFIDUS1982.6.2_55","DOIUrl":"https://doi.org/10.12938/BIFIDUS1982.6.2_55","url":null,"abstract":"The fecal bacterial flora of three healthy Thai residents in Japan were determined . Two were short-term or long-term vegetarians and one was a mixed-diet omnivore . The total bacterial counts of all these subjects were significantly lower than those of the average Japanese. The total counts and the numbers of anaerobic bacteria in the vegetarians were constant, whereas a fluctuation between the omnivore's two samplings was observed, accompanied with a decrease in bacteroidaceae , eubacteria, bifidobacteria, enterococci, and veillonellae, and an increase in Clostridium perfringens , lactobacilli, and staphylococci.","PeriodicalId":414713,"journal":{"name":"Bifidobacteria and Microflora","volume":"70 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127353748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.12938/BIFIDUS1982.10.2_131
T. Chida, N. Okamura, R. Nakaya, Y. Benno, T. Mitsuoka
The in vitro activity of selected antibiotics were determined for the strains of Bacteroides vulgatus and B. fragilis isolated from the feces and bowel tissue specimens of patients with ulcerative colitis (UC) and from the feces of healthy adults by an agar dilution method. Significant differences in resistance rates to ampicillin, tetracycline, and macrolide-lincosamide were observed between the strains of B. vulgatus, but not B. fragilis, isolated from the patients with UC and those from the healthy adults.
{"title":"Comparison of Antimicrobial Susceptibility of the Bacteroides vulgatus and B. fragilis Strains Isolated from Feces or Bowel Tissue Specimens of Patients with Ulcerative Colitis and Those from Feces of Healthy Adults","authors":"T. Chida, N. Okamura, R. Nakaya, Y. Benno, T. Mitsuoka","doi":"10.12938/BIFIDUS1982.10.2_131","DOIUrl":"https://doi.org/10.12938/BIFIDUS1982.10.2_131","url":null,"abstract":"The in vitro activity of selected antibiotics were determined for the strains of Bacteroides vulgatus and B. fragilis isolated from the feces and bowel tissue specimens of patients with ulcerative colitis (UC) and from the feces of healthy adults by an agar dilution method. Significant differences in resistance rates to ampicillin, tetracycline, and macrolide-lincosamide were observed between the strains of B. vulgatus, but not B. fragilis, isolated from the patients with UC and those from the healthy adults.","PeriodicalId":414713,"journal":{"name":"Bifidobacteria and Microflora","volume":"11 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114174415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.12938/BIFIDUS1982.9.2_87
I. Suzuki, K. Hashimoto, T. Yadomae
The preparation of cells of Peyer's patches (PP) from mice and some immunological activities of the cells were examined. The PP cells were obtained with high yield (> 1 •~ 107/CDF1 mouse) and viability (>97%), when the PP were cut and teased gently with two glasses in RPMI 1640 medium containing 5% heat-inactivated calf serum. The PP cells responded well to mitogens, and particularly the responses to a T cell mitogen, concanavalin A (Con A), were at similar levels to those of spleen cells. When the cells were stimulated with alloantigens in mixed lymphocyte reaction, significant response was observed. The PP cells cultured with lipopolysaccharide (LPS) exhibited polyclonal B cell responses (antigen-nonspecific increase in the number of antibody-producing cells) . Furthermore, the levels of anti sheep erythrocytes (SRBC) plaque-forming cells (PFC) in PP cell cultures increased significantly when the PP cells were cultured with SRBC in vitro. However, the PP cells did not show the natural killer (NK) activity. These results showed that the PP cells obtained by the physical method described here contained functionable T and B lymphocytes and accessory cells.
{"title":"Rapid Preparation of Functional Murine Peyer's Patch Cells","authors":"I. Suzuki, K. Hashimoto, T. Yadomae","doi":"10.12938/BIFIDUS1982.9.2_87","DOIUrl":"https://doi.org/10.12938/BIFIDUS1982.9.2_87","url":null,"abstract":"The preparation of cells of Peyer's patches (PP) from mice and some immunological activities of the cells were examined. The PP cells were obtained with high yield (> 1 •~ 107/CDF1 mouse) and viability (>97%), when the PP were cut and teased gently with two glasses in RPMI 1640 medium containing 5% heat-inactivated calf serum. The PP cells responded well to mitogens, and particularly the responses to a T cell mitogen, concanavalin A (Con A), were at similar levels to those of spleen cells. When the cells were stimulated with alloantigens in mixed lymphocyte reaction, significant response was observed. The PP cells cultured with lipopolysaccharide (LPS) exhibited polyclonal B cell responses (antigen-nonspecific increase in the number of antibody-producing cells) . Furthermore, the levels of anti sheep erythrocytes (SRBC) plaque-forming cells (PFC) in PP cell cultures increased significantly when the PP cells were cultured with SRBC in vitro. However, the PP cells did not show the natural killer (NK) activity. These results showed that the PP cells obtained by the physical method described here contained functionable T and B lymphocytes and accessory cells.","PeriodicalId":414713,"journal":{"name":"Bifidobacteria and Microflora","volume":"86 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124956108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.12938/BIFIDUS1982.7.1_19
T. Miwatani, M. Kohda, T. Honda
Two important steps are involved in the pathogenesis of bacterial enteric pathogens. All the enteric pathogens, which enter the gastrointestinal tract through the mouth with food or water, colonize a certain part of the intestine. This important step is called colonization (4, 9) . For example, Vibrio cholerae and Vibrio parahaemolyticus colonize the mucosa of the upper jejunum, whereas Shigella colonizes the ileocaecum and colon. This event (colonization) is mediated by the production of specific surface antigens called "colonization factors" and specific receptors for the factors. The reason for the differences between the symptoms produced by V. cholerae O1 and V. parahaemolyticus, both of which colonize a similar region of the small intestine, is that they produce different toxins. Thus the second important step in the pathogenesis of bacterial enteric pathogens is the production of toxins and/or invasion.
{"title":"Pathogenic Mechanisms of Bacterial Enteropathogens","authors":"T. Miwatani, M. Kohda, T. Honda","doi":"10.12938/BIFIDUS1982.7.1_19","DOIUrl":"https://doi.org/10.12938/BIFIDUS1982.7.1_19","url":null,"abstract":"Two important steps are involved in the pathogenesis of bacterial enteric pathogens. All the enteric pathogens, which enter the gastrointestinal tract through the mouth with food or water, colonize a certain part of the intestine. This important step is called colonization (4, 9) . For example, Vibrio cholerae and Vibrio parahaemolyticus colonize the mucosa of the upper jejunum, whereas Shigella colonizes the ileocaecum and colon. This event (colonization) is mediated by the production of specific surface antigens called \"colonization factors\" and specific receptors for the factors. The reason for the differences between the symptoms produced by V. cholerae O1 and V. parahaemolyticus, both of which colonize a similar region of the small intestine, is that they produce different toxins. Thus the second important step in the pathogenesis of bacterial enteric pathogens is the production of toxins and/or invasion.","PeriodicalId":414713,"journal":{"name":"Bifidobacteria and Microflora","volume":"7 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129332709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.12938/BIFIDUS1982.10.2_113
D. Hoover, D. B. Hughes
Bifidobacteria have been known and studied throughout the 20th century. The early work originated at the Pasteur Institute. Since that time basic and applied research on bifidobacteria have been done worldwide, with a portion carried out in the U.S. Presently Japan dominates the study and development of bifidobacteria for human use. This article examines some of the possible reasons for the minor role of the U.S. in the study of bifidobacteria and development of bifidobacteria-amended products, with a discussion of future trends in this area.
{"title":"Current Status and Future Trends of Bifidobacteria-related Research and Products in the USA","authors":"D. Hoover, D. B. Hughes","doi":"10.12938/BIFIDUS1982.10.2_113","DOIUrl":"https://doi.org/10.12938/BIFIDUS1982.10.2_113","url":null,"abstract":"Bifidobacteria have been known and studied throughout the 20th century. The early work originated at the Pasteur Institute. Since that time basic and applied research on bifidobacteria have been done worldwide, with a portion carried out in the U.S. Presently Japan dominates the study and development of bifidobacteria for human use. This article examines some of the possible reasons for the minor role of the U.S. in the study of bifidobacteria and development of bifidobacteria-amended products, with a discussion of future trends in this area.","PeriodicalId":414713,"journal":{"name":"Bifidobacteria and Microflora","volume":"25 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129059032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.12938/BIFIDUS1982.4.1_23
M. Yamashita, M. Hatano, Hajime Nakamura, R. Murakami, M. Matsuo, T. Matsuo, Yasushi Kumon
For the purpose of investigating the effects of Biofermin-R (BF-R) on the bacterial flora, BF-R was administered in combination with antibiotics , and the fecal flora of children treated with antibiotics alone was compared with that of children treated with both BF-R and antibiotics. Three types of effects were investigated: 1) the inhibitory effect on antibiotic-induced changes of the bacterial flora in patients without diarrhea , 2) the bacterial flora-maintaining and normalizing effect in patients with gastrointestinal symptoms, and 3) the process of normalizing in the fecal bacterial flora of mice administered with antibiotics. The results indicated that the concurrent use of BF-R and an antibiotic inhibited the changes of the intestinal flora that usually occur during antibiotic therapy alone, by preventing a decrease in Bifidobacterium, and restored disturbed flora to normal.
为了研究生物铁蛋白- r (BF-R)对细菌菌群的影响，我们将BF-R与抗生素联合使用，并比较单独使用抗生素的儿童与同时使用BF-R和抗生素的儿童的粪便菌群。研究了三种作用:1)抗生素对非腹泻患者细菌菌群变化的抑制作用;2)胃肠道症状患者细菌菌群维持和正常化作用;3)抗生素给药小鼠粪便细菌菌群的正常化过程。结果表明，BF-R与抗生素同时使用，通过防止双歧杆菌的减少，抑制了抗生素单独治疗时肠道菌群的变化，使受干扰的菌群恢复正常。
{"title":"Effects of Biofermin-R Administered in Combination with Antibiotics on the Fecal Flora","authors":"M. Yamashita, M. Hatano, Hajime Nakamura, R. Murakami, M. Matsuo, T. Matsuo, Yasushi Kumon","doi":"10.12938/BIFIDUS1982.4.1_23","DOIUrl":"https://doi.org/10.12938/BIFIDUS1982.4.1_23","url":null,"abstract":"For the purpose of investigating the effects of Biofermin-R (BF-R) on the bacterial flora, BF-R was administered in combination with antibiotics , and the fecal flora of children treated with antibiotics alone was compared with that of children treated with both BF-R and antibiotics. Three types of effects were investigated: 1) the inhibitory effect on antibiotic-induced changes of the bacterial flora in patients without diarrhea , 2) the bacterial flora-maintaining and normalizing effect in patients with gastrointestinal symptoms, and 3) the process of normalizing in the fecal bacterial flora of mice administered with antibiotics. The results indicated that the concurrent use of BF-R and an antibiotic inhibited the changes of the intestinal flora that usually occur during antibiotic therapy alone, by preventing a decrease in Bifidobacterium, and restored disturbed flora to normal.","PeriodicalId":414713,"journal":{"name":"Bifidobacteria and Microflora","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131263807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.12938/BIFIDUS1982.6.2_59
Y. Benno, K. Endo, N. Shiragami, K. Sayama, Tomotari Mitsuokai
The effects of four-week raffinose intake (15 g/day) on the fecal microflora and fecal properties were studied in healthy human volunteers. The significantly increasing numbers of Bifidobacterium spp. were observed during the raffinose intake, whereas the numbers of lecithinase-negative Clostridium spp. and bacteroidaceae during the intake were significantly lower than those before and after the intake. The percentage of Bifidobacterium spp. was increased from 11.6-15.5% of the total to 58.2-80.1% of the total during the intake. The fecal pH values during the feeding were lower than those before and after the intake.
{"title":"Effects of Raffinose Intake on Human Fecal Microflora","authors":"Y. Benno, K. Endo, N. Shiragami, K. Sayama, Tomotari Mitsuokai","doi":"10.12938/BIFIDUS1982.6.2_59","DOIUrl":"https://doi.org/10.12938/BIFIDUS1982.6.2_59","url":null,"abstract":"The effects of four-week raffinose intake (15 g/day) on the fecal microflora and fecal properties were studied in healthy human volunteers. The significantly increasing numbers of Bifidobacterium spp. were observed during the raffinose intake, whereas the numbers of lecithinase-negative Clostridium spp. and bacteroidaceae during the intake were significantly lower than those before and after the intake. The percentage of Bifidobacterium spp. was increased from 11.6-15.5% of the total to 58.2-80.1% of the total during the intake. The fecal pH values during the feeding were lower than those before and after the intake.","PeriodicalId":414713,"journal":{"name":"Bifidobacteria and Microflora","volume":"65 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134139018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.12938/BIFIDUS1982.11.1_25
T. Yaeshima, T. Fujisawa, T. Mitsuoka
Seventy-six strains of Bifidobacterium adolescentis and phenotypically similar Bifidobacterium species, of which 56 strains were isolated from the feces of human adults in our laboratory and 20 strains were obtained from DSM and ATCC as type strains or reference strains, were studied by carbohydrate fermentation patterns, DNA base compositions and DNA/DNA homologies. On the basis of DNA/DNA homology they were divided into six species: B. adolescentis, B. pseudocatenulatum, B. catenulatum, B. angulatum, B. dentium and B. longum. The strains of B. angulatum and B. dentium were not detected in 56 strains isolated from the feces of human adults. B. catenulatum could be distinguished from other four species, except for B. longum, by the inability to ferment glycogen and mannose. The G+C contents of DNAs of B. pseudocatenulatum and B. catenulatum (57.1•}0.7 mol% and 56.5•}1.0 mol%, respectively) were lower than that of B. adolescentis (59.8•}0.9 mol%). It was difficult to differentiate B. adolescentis biovar b and B. dentium, and B. adolescentis biovar c and B. pseudocatenulatum on the basis of carbohydrate fermentation pattern.
{"title":"Bifidobacterium Species Expressing Phenotypical Similarity to Bifidobacterium adolescentis Isolated from the Feces of Human Adults","authors":"T. Yaeshima, T. Fujisawa, T. Mitsuoka","doi":"10.12938/BIFIDUS1982.11.1_25","DOIUrl":"https://doi.org/10.12938/BIFIDUS1982.11.1_25","url":null,"abstract":"Seventy-six strains of Bifidobacterium adolescentis and phenotypically similar Bifidobacterium species, of which 56 strains were isolated from the feces of human adults in our laboratory and 20 strains were obtained from DSM and ATCC as type strains or reference strains, were studied by carbohydrate fermentation patterns, DNA base compositions and DNA/DNA homologies. On the basis of DNA/DNA homology they were divided into six species: B. adolescentis, B. pseudocatenulatum, B. catenulatum, B. angulatum, B. dentium and B. longum. The strains of B. angulatum and B. dentium were not detected in 56 strains isolated from the feces of human adults. B. catenulatum could be distinguished from other four species, except for B. longum, by the inability to ferment glycogen and mannose. The G+C contents of DNAs of B. pseudocatenulatum and B. catenulatum (57.1•}0.7 mol% and 56.5•}1.0 mol%, respectively) were lower than that of B. adolescentis (59.8•}0.9 mol%). It was difficult to differentiate B. adolescentis biovar b and B. dentium, and B. adolescentis biovar c and B. pseudocatenulatum on the basis of carbohydrate fermentation pattern.","PeriodicalId":414713,"journal":{"name":"Bifidobacteria and Microflora","volume":"11 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129653598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.12938/BIFIDUS1982.10.1_19
S. Yamazaki, S. Tsuyuki, H. Akashiba, H. Kamimura, M. Kimura, T. Kawashima, K. Ueda
Various investigators have discussed floral microorganisms influence on a host's immune system (4, 6, 9). Most of these statements come from investigations comparing responses of germfree animals and flora-bearing ones. The present authors attempted to analyze the effect of an indigenous floral organisms on the host under a more simplified condition. Bifidobacterium longum (BL) was given per os to germfree mice (GF) of BALB/c background and various aspects of immune responses to the associated organism were investigated first; antibody (mainly IgA) and cell-mediated immune (CMI) responses to BL, translocations of BL into the internal organs, and shift of lymphocyte subsets in the lymphoid organs were found following per os administration of BL. Some activities of BL on in vitro cultured lymphocytes were also found. Additionally, it was shown that the BL-monoassociated mouse (GB) exhibits beneficial effects in respect to specific immune response and non-specific antibacterial defence reactions to unrelated antigenic substances. These non-specific effects were seen on lethal activity of Escherichia coliendotoxin, IgA production to cholera toxin (CT), and induction of oral tolerance to unrelated antigens.
{"title":"Immune Response of Bifidobacterium-Monoassociated Mice","authors":"S. Yamazaki, S. Tsuyuki, H. Akashiba, H. Kamimura, M. Kimura, T. Kawashima, K. Ueda","doi":"10.12938/BIFIDUS1982.10.1_19","DOIUrl":"https://doi.org/10.12938/BIFIDUS1982.10.1_19","url":null,"abstract":"Various investigators have discussed floral microorganisms influence on a host's immune system (4, 6, 9). Most of these statements come from investigations comparing responses of germfree animals and flora-bearing ones. The present authors attempted to analyze the effect of an indigenous floral organisms on the host under a more simplified condition. Bifidobacterium longum (BL) was given per os to germfree mice (GF) of BALB/c background and various aspects of immune responses to the associated organism were investigated first; antibody (mainly IgA) and cell-mediated immune (CMI) responses to BL, translocations of BL into the internal organs, and shift of lymphocyte subsets in the lymphoid organs were found following per os administration of BL. Some activities of BL on in vitro cultured lymphocytes were also found. Additionally, it was shown that the BL-monoassociated mouse (GB) exhibits beneficial effects in respect to specific immune response and non-specific antibacterial defence reactions to unrelated antigenic substances. These non-specific effects were seen on lethal activity of Escherichia coliendotoxin, IgA production to cholera toxin (CT), and induction of oral tolerance to unrelated antigens.","PeriodicalId":414713,"journal":{"name":"Bifidobacteria and Microflora","volume":"25 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122564824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.12938/BIFIDUS1982.13.1_1
K. Hirayama, M. Mishima, S. Kawamura, K. Itoh, E. Takahashi, T. Mitsuoka
Human-flora-associated (HFA) mice produced by oral inoculation with human fecal suspension to germfree mice were examined on the changes of the composition of fecal flora when they were given high-meat (HM) and high-bran (HB) diet and fructooligosaccharides (FOS) . The composition of fecal flora of HFA mice was similar to that of the inoculated human feces. Number of enterobacteriaceae significantly increased with HM diet and decreased with HB diet to the level with basal diet. Bacteroidaceae, clostridia and streptococci decreased their numbers with HB diet, while the number of bifidobacteria in HFA mice fed HB diet was significantly higher than those fed HM or basal diet. The rate of bifidobacteria to total bacteria increased and the numbers of bacteroidaceae and enterobacteriaceae tended to decrease in HFA mice administered FOS solution, but the differences were not statistically significant. These results indicate that the changes of floral composition by different dietary components can be observed more obviously in HFA mice than in humans.
{"title":"Effects of dietary supplements on the composition of fecal flora of human-flora-associated (HFA) mice","authors":"K. Hirayama, M. Mishima, S. Kawamura, K. Itoh, E. Takahashi, T. Mitsuoka","doi":"10.12938/BIFIDUS1982.13.1_1","DOIUrl":"https://doi.org/10.12938/BIFIDUS1982.13.1_1","url":null,"abstract":"Human-flora-associated (HFA) mice produced by oral inoculation with human fecal suspension to germfree mice were examined on the changes of the composition of fecal flora when they were given high-meat (HM) and high-bran (HB) diet and fructooligosaccharides (FOS) . The composition of fecal flora of HFA mice was similar to that of the inoculated human feces. Number of enterobacteriaceae significantly increased with HM diet and decreased with HB diet to the level with basal diet. Bacteroidaceae, clostridia and streptococci decreased their numbers with HB diet, while the number of bifidobacteria in HFA mice fed HB diet was significantly higher than those fed HM or basal diet. The rate of bifidobacteria to total bacteria increased and the numbers of bacteroidaceae and enterobacteriaceae tended to decrease in HFA mice administered FOS solution, but the differences were not statistically significant. These results indicate that the changes of floral composition by different dietary components can be observed more obviously in HFA mice than in humans.","PeriodicalId":414713,"journal":{"name":"Bifidobacteria and Microflora","volume":"40 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122675997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：